KR20010018668A - A cosmetic composition containing Cinnamomi Cortex extracts - Google Patents
A cosmetic composition containing Cinnamomi Cortex extracts Download PDFInfo
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- KR20010018668A KR20010018668A KR1019990034707A KR19990034707A KR20010018668A KR 20010018668 A KR20010018668 A KR 20010018668A KR 1019990034707 A KR1019990034707 A KR 1019990034707A KR 19990034707 A KR19990034707 A KR 19990034707A KR 20010018668 A KR20010018668 A KR 20010018668A
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- A—HUMAN NECESSITIES
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A61Q1/00—Make-up preparations; Body powders; Preparations for removing make-up
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q5/00—Preparations for care of the hair
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Abstract
Description
본 발명은 녹나무과(Lauraceae) 식물인 육계(Cinnanmonum cassia Blume)의 수피 추출물인 육계피(Cinnamomi Cortex) 추출물을 함유하는 화장료 조성물에 관한 것이다.The present invention relates to a cosmetic composition containing an extract of Cinnamon (Cinnamomi Cortex) which is a bark extract of Cinnanmonum cassia Blume, which is a Lauraceae plant.
화장품관련 분야에서 피부의 노화를 억제하기 위하여 극복하여야 할 과제중 자유라디칼(Free radical)에 의한 산화적 스트레스(Oxidative stress)는 가장 중요한 문제이다. 자유라디칼에 의한 손상은 인체 내에서 광범위하게 발생하여 노화 발생의 주 기작으로 인식되고 있다.In the cosmetic field, oxidative stress caused by free radicals is the most important problem to overcome in order to suppress skin aging. Damage caused by free radicals is widely recognized in the human body and is recognized as the main mechanism of aging.
자유라디칼은 하나 이상의 짝을 이루지 못한 전자를 가지고 있는 원소 또는 분자로서 다른 분자로부터 전자를 뺏어 안정화하려는 특성을 지니고 있다. 자유라디칼의 수명은 수천분의 일초정도로 매우 짧으며, 불안정하여 매우 공격적인 성질을 나타낸다.Free radicals are elements or molecules with one or more unpaired electrons and have the property of stabilizing electrons from other molecules. The lifetime of free radicals is very short (a few thousandths of a second), unstable and very aggressive.
자유라디칼은 생체내에서 자연적으로 발생하는 물질인데 자연발생된 라디칼은 생체내의 라디칼에 대한 시스템과의 균형적인 작용에 의해 유해한 반응없이 해소된다. 자유라디칼의 생체내 발생경로는 미토콘드리아(Mitochondria)의 전자전달체계(Electron Transport System), 대식작용(Phagocytosis), 해독작용(Detoxification) 및 프로스타글란딘 대사작용(Prostagrandin metabolism) 등이 있다. 또한 공해물질, 흡연, 약물복용, 자외선 등에 의한 외부요인의 병리적인 현상에 의해서도 라디칼은 생성되는데 특히 이들 라디칼에 의한 산화적 스트레스는 생체내의 방어 시스템을 약화시켜 세포내의 산화-환원 균형을 파괴시켜 피부의 노화, 구체적으로는 광노화를 촉진시킨다.Free radicals are substances that occur naturally in vivo, and naturally occurring radicals are resolved without harmful reactions by a balanced action with the system on radicals in vivo. In vivo developmental pathways of free radicals include the Mitochondria Electron Transport System, Phagocytosis, Detoxification and Prostagrandin metabolism. In addition, radicals are produced by pathological phenomena of external factors such as pollutants, smoking, drug use, and ultraviolet rays. Aging, specifically photoaging.
생체내에서 생성되는 라디칼로 대표적인 히드록실 라디칼(Hydroxyl radical), 슈퍼옥사이드 라디칼(Superoxide radical) 및 활성산소(Singlet oxygen) 등은 강한 반응력을 가지고 있으며, 생체의 모든 물질과 반응할수 있다. 지질(Lipid)을 공격하여 지질과산화물(Lipid peroxide)을 생성시키며, 세포막의 성질을 변화시킨다. 세포막 성질의 변화는 세포막을 통한 물질들의 전달체계의 변화로 이어져 노화를 촉진시킨다. 단백질을 공격하여 단백질을 변성시키거나, 단백질의 기능을 약화시킨다. 또한 유전정보인 DNA를 공격하여 세포를 불활성화시킨다. 결국 피부의 세포들은 라디칼에 계속적인 노출시 노화가 촉진된다.Representative hydroxyl radicals, superoxide radicals and active oxygen (Singlet oxygen), etc. are radicals generated in vivo and have a strong reaction force, and can react with all substances of the living body. Attacks lipids to produce lipid peroxides and changes the properties of cell membranes. Changes in cell membrane properties lead to changes in the delivery system of substances through the cell membrane, thereby promoting aging. Attacks proteins to denature proteins or to weaken their function. It also inactivates cells by attacking DNA, which is genetic information. Eventually, the skin's cells promote aging upon subsequent exposure to radicals.
인체에는 자유라디칼에 의한 손상을 방지하기 위한 방어 시스템을 갖추고 있지만 대기오염, 불건전한 식생활, 자외선 노출, 스트레스 및 질병들의 유해환경에 노출되어 생체내의 라디칼 소거 시스템과의 불균형으로 여러 라디칼들에 의한 산화적 스트레스(Oxidative stress)를 충분히 소거하기 어려운 형편이다. 이런 이유로 라디칼에 의한 산화적 스트레스는 피부의 노화, 태양광에 의한 화상 및 염증, 접촉성 피부염, 주름 등을 발생시킨다. 따라서 자유라디칼을 소거할 수 있는 물질은 피부에 대한 산화적 스트레스를 제거하여 피부의 노화를 방지 또는 지연할 수 있다.Although the human body has a defense system to prevent damage caused by free radicals, it is exposed to harmful environment such as air pollution, unhealthy diet, UV exposure, stress, and diseases, and is oxidized by various radicals due to imbalance with the radical scavenging system in the body. It's hard to get rid of enough stress. For this reason, oxidative stress caused by radicals causes skin aging, sunburn and inflammation caused by sunlight, contact dermatitis and wrinkles. Therefore, the substance capable of eliminating free radicals may prevent or delay aging of the skin by removing oxidative stress on the skin.
이에 본 발명자들은 식물로부터 자유라디칼 소거 물질을 개발하기 위하여 우리나라에서 자생하고 있는 생약에 대한 탐색 연구를 수행한 결과, 육계피 추출물의 자유라디칼 소거 효과가 매우 우수함을 발견하여 본 발명을 완성하게 되었다.Accordingly, the present inventors conducted a search study on herbal medicines growing in Korea in order to develop a free radical scavenging substance from plants. As a result, the inventors found that the free radical scavenging effect of cinnamon extract was very excellent, thereby completing the present invention.
따라서, 본 발명은 육계피 추출물을 함유하는 화장료 조성물을 제공하는 것을 목적으로 한다.Therefore, an object of the present invention is to provide a cosmetic composition containing a cinnamon extract.
본 발명은 육계피(Cinnamomi Cortex) 추출물을 함유하는 화장료 조성물에 관한 것이다.The present invention relates to a cosmetic composition containing the extract cinnamon (Cinnamomi Cortex).
본 발명에 따른 화장료 조성물에 특징적으로 함유되는 육계피 추출물은 녹나무과(Lauraceae) 식물인 육계(Cinnanmonum cassia Blume)의 수피로부터 추출된 것이다. 육계는 상록교목으로 긴 타원형 잎이 어긋나게 붙어 있다. 잎에는 3개의 잎줄이 활처럼 뻗어 있고, 노란색의 작은 꽃이 핀다. 수피(樹皮)는 외 표면이 회갈색이고 피공(皮孔)이 타원형이며 향기가 있고 맛이 달다. 육계의 수피는 8월 내지 10월경에 채취하여 햇볕에 말린다. 육계의 수피 즉, 육계피는 판산 또는 양갓이 안쪽으로 말린 판상으로 길이 30cm 내지 50㎝, 너비 5cm 내지 12㎝, 두께 4mm 내지 8㎜이다. 바깥면은 회갈색이고 거칠고 세포 잔주름과 옆으로 피공이 돌출되어 있다. 그리고 회백색의 반점이 군데군데 있고 안쪽면은 적색 또는 갈색이다. 꺽은 면은 과립상이고 가운데에서 백색의 석세포 층을 볼 수 있다. 횡단면을 현미경으로 관찰할 때 유세포의 막은 적색이고, 백색의 석세포 및 석세포 관층을 볼 수 있다. 육계의 질은 단단하며, 특이한 방향이 있고 맛은 맵고 달다.Cinnamon extract characteristically contained in the cosmetic composition according to the present invention is extracted from the bark of the Cinnamon (Cinnanmonum cassia Blume), which is a plant of the family Lauraceae. Broilers are evergreen arbors with long oval leaves alternately attached. The leaves have three leaf lines extending like a bow and small yellow flowers bloom. The bark is grayish brown on the outer surface, oval in the shape of pores, fragrant and sweet. Broiler bark is harvested from August to October and dried in the sun. The bark of broilers, or broiled cinnamon, is 30cm to 50cm long, 5cm to 12cm wide, and 4mm to 8mm thick in the form of a plate that is rolled inwardly or by pancakes. The outer side is grayish brown and rough, and the fine lines of the cells and the pores protrude laterally. There are several spots of grayish white spots and the inside is red or brown. The facets are granular, with a white layer of masonry cells in the middle. When the cross section is observed under a microscope, the membrane of the flow cell is red, and the white stone cells and the stone cell tube layer can be seen. The broiler's quality is hard, has an unusual aroma and tastes hot and sweet.
본 발명에 따른 화장료 조성물에 함유되는 육계피 추출물은 분말화된 육계피로부터 물, 메탄올, 메탄올 수용액, 에탄올 에탄올 수용액, 아세톤, 에틸아세테이트, 1,3-부틸렌 글리콜 또는 1,3-부틸렌글리콜 수용액, 헥산, 디에틸 에테르, 노말 프로판올, 이소 프로판올 또는 노말 부탄올을 추출용매로 사용하여 추출하고 감압농축하여 제조할 수 있다.The cinnamon extract contained in the cosmetic composition according to the present invention is water, methanol, methanol aqueous solution, ethanol ethanol aqueous solution, acetone, ethyl acetate, 1,3-butylene glycol or 1,3-butylene glycol from powdered cinnamon Aqueous solution, hexane, diethyl ether, normal propanol, iso propanol or normal butanol can be extracted using an extraction solvent and concentrated under reduced pressure.
상기 육계피 추출물은 상기 용매하에서 15℃ 내지 40℃에서 1 내지 15일간 침적시키거나 냉각콘덴서가 장착된 추출기에서 1내지 7일간 추출한 후, 감압농축하여 제조할 수 있다. 또한 추출공정 수행후 4내지 15℃에서 7 ~ 10 일간 저온숙성하는 공정을 포함할 수 있다.The cinnamon extract may be prepared by immersing at 15 ° C. to 40 ° C. under the solvent for 1 to 15 days or extracting the extract from 1 to 7 days in an extractor equipped with a cooling capacitor and then concentrating under reduced pressure. In addition, the extraction process may include a low-temperature aging process for 7 to 10 days at 4 to 15 ℃.
상기 육계피 추출물의 제조방법에 있어서, 추출용매는 육계피(Cinnamimi Cortex) 건조중량에 대하여 1배 내지 15배의 양으로 사용하는 것이 바람직하다.In the method of preparing the cinnamon extract, the extraction solvent is preferably used in an amount of 1 to 15 times the dry weight of the cinnamon (Cinnamimi Cortex).
상기와 같이 제조된 육계피 추출물은 통상의 첨가제를 가하여 통상의 방법에 의해 화장료 조성물로 제조될 수 있으며, 화장료 건조량에 대하여 0.001중량내지 10중량, 더욱 바람직하게는 1중량내지 7중량로 함유되는 것이 바람직하다.The cinnamon extract prepared as described above may be prepared in a cosmetic composition by a conventional method by adding a conventional additive, it is contained in an amount of 0.001 to 10 weight, more preferably 1 to 7 weight based on the dry amount of the cosmetic desirable.
본 발명에 따른 화장료 조성물은 유연 화장수, 밀크로션, 영양크림, 맛사지 크림, 에센스, 클렌싱 폼, 클렌싱 워터, 팩 또는 보디오일 등의 기초 화장료 형태로 제조할 수 있다.The cosmetic composition according to the present invention may be prepared in the form of a basic cosmetic such as flexible lotion, milk lotion, nourishing cream, massage cream, essence, cleansing foam, cleansing water, pack or body oil.
또한 본 발명에 따른 화장료 조성물은 화운데이션, 립스틱, 마스카라 또는 메이크업 베이스 등의 색조 화장료 형태로 제조할 수 있다.In addition, the cosmetic composition according to the present invention can be prepared in the form of color cosmetics, such as foundation, lipstick, mascara or makeup base.
또한 본 발명에 따른 화장료 조성물은 샴푸, 린스, 헤어콘디셔너 또는 헤어젤 등의 두발용 화장료 형태로 제조할 수 있다.In addition, the cosmetic composition according to the present invention can be prepared in the form of a hair cosmetic such as shampoo, conditioner, hair conditioner or hair gel.
이하 실시예, 실험예, 비교예 및 처방예를 통하여 본 발명을 더욱 상세히 설명한다. 그러나 본 발명이 이에 한정되는 것은 아니다.Hereinafter, the present invention will be described in more detail with reference to Examples, Experimental Examples, Comparative Examples, and Prescription Examples. However, the present invention is not limited thereto.
실시예 1Example 1
정제수로 세척하고 건조시킨 다음 분말화한 육계피 가루 1kg을 물 5ℓ에 넣고 20℃에서 5일간 추출한 후 300메쉬 여과포로 여과하고, 10℃에서 10일간 방치하여 저온 숙성시킨 후, 와트만 2번 여과지로 여과하였다. 이 추출물을 냉각 콘덴서가 달린 증류장치에서 60℃로 감압 농축하고 건조하여 24.1g(건조중량)을 제조하였다.After washing with purified water and drying, 1kg of powdered cinnamon powder was put in 5ℓ of water, extracted at 20 ° C for 5 days, filtered through 300 mesh filter cloth, left at 10 ° C for 10 days, and aged at low temperature. Was filtered. The extract was concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling condenser and dried to prepare 24.1 g (dry weight).
실시예 2Example 2
정제수로 세척하고 건조시킨 다음 분말화한 육계피 가루 1kg을 25에탄올 5ℓ에 넣고 냉각 콘덴서가 달린 추출기에서 3일간 추출한 후 300메쉬 여과포로 여과하고 10℃에서 10일간 방치하여 저온 숙성시킨 후 와트만 2번 여과지로 여과하였다. 이 추출물은 냉각 콘덴서가 달린 증류장치에서 60℃로 감압 농축하고 건조하여 38.6g(건조중량)을 제조하였다.After washing with purified water and drying, 1 kg of powdered cinnamon powder was placed in 5 liters of 25 ethanol, and extracted for 3 days in an extractor equipped with a cooling condenser. Filtration was carried out by filter paper. The extract was concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling condenser and dried to prepare 38.6 g (dry weight).
실시예 3∼15Examples 3-15
하기 표 1 기재의 용매를 사용하여 실시예 2의 방법으로 추출하여 하기 표 1 기재와 같이 건조중량을 제조하였다.To extract the dry weight by the method of Example 2 using a solvent described in Table 1 below as described in Table 1.
실험예 1Experimental Example 1
육계피 추출물의 자유라디칼 소거 효과Free Radical Scavenging Effect of Cinnamon Extract
1) 실험 방법1) Experiment Method
자유라디칼 소거(free radical scavenging)작용은 60μM 1,1-디페닐-2-피크릴 히드라질(1,1-diphenyl-2-picryl hydrazyl; DPPH) 에탄올 용액 2㎖에 육계피 추출물의 디메틸설폭시드(DMSO) 용액 2㎖를 가해 30분간 실온에서 반응시킨 후, 520nm에서 흡광도를 측정하여 자유라디칼 소거 효과()를 구하였다. 각 실시예 추출물의 자유라디칼 소거 효과는 하기의 공식으로 계산하였다.The free radical scavenging effect is the dimethyl sulfoxide of cinnamon extract in 2 ml of 60 μM 1,1-diphenyl-2-picryl hydrazyl (DPPH) ethanol solution. After 2 ml of (DMSO) solution was added and reacted at room temperature for 30 minutes, the absorbance was measured at 520 nm to determine the free radical scavenging effect (). The free radical scavenging effect of each example extract was calculated by the following formula.
자유라디칼 소거 효과()= 100 - [(추출물 처리후 흡광도/대조군 흡광도) × 100]Free radical scavenging effect () = 100-[(absorbance after extract treatment / control absorbance) × 100]
2) 실험 결과2) Experiment result
육계피 추출물의 자유라디칼 소거 효과는 표 2에 나타내었다. 육계피 추출물들은 대부분 높은 활성을 나타내었다.The free radical scavenging effect of the extract of Cinnamon is shown in Table 2. Cinnamon extracts showed high activity.
* 상기 값은 3회 실시 평균값임* The above value is the average of 3 runs
비교예 1Comparative Example 1
육계피 추출물은 실시예 6에서 제조한 것을 사용하였다. 육계피 추출물과 기존 소거제들과의 자유라디칼 소거 효과를 실험예 1과 같은 방법으로 측정하였으며 SC50값으로 비교한 결과를 표 3에 나타내었다. SC50은 자유라디칼을 50소거하기 위해 필요한 시료의 농도를 말한다.Cinnamon extract was prepared in Example 6. The free radical scavenging effect of the extract of cinnamon and the existing scavengers was measured in the same manner as in Experimental Example 1, and the results of comparing the SC 50 values to the SC 50 are shown in Table 3. SC 50 is the concentration of sample required to remove 50 free radicals.
육계피 추출물의 자유라디칼 소거 효과를 기존의 소거제들과 비교한 결과 육계피 추출물이 매우 높은 활성을 나타내었다.The free radical scavenging effect of the cinnamon extract was compared with the existing scavengers, and the cinnamon extract showed very high activity.
실험예 2Experimental Example 2
육계피 추출물의 과산화 수소(H2O2) 유도 세포 독성 억제 효과Inhibitory Effects of Cinnamon Extracts on Hydrogen Peroxide (H 2 O 2 ) -induced Cytotoxicity
육계피 추출물의 과산화 수소(H2O2) 유도 세포 독성에 대한 억제 효과를 마우스 유래 피부 섬유아세포(fibroblast)를 사용하여 측정하였다. 과산화 수소는 세포내에서 히드록실 라디칼을 생성하여 세포에 치명적 손상을 일으킨다. 육계피 추출물의 자유라디칼 소거 효과를 세포 수준에서 시험하기 위하여 다음과 같이 측정하였다.Inhibitory effect of the extract of cinnamon on hydrogen peroxide (H 2 O 2 ) -induced cytotoxicity was measured using mouse-derived skin fibroblasts. Hydrogen peroxide produces hydroxyl radicals in the cell, causing fatal damage to the cell. Free radical scavenging effect of cinnamon extract was measured as follows to test at the cellular level.
1) 실험 방법1) Experiment Method
육계피 추출물은 실시예 6에서 제조한 것을 사용하였다. 마우스 유래 섬유아세포(fibroblast, NIH/3T3)를 각 웰(well)에 5×104개를 96웰 플레이트에 접종하여 디엠이엠(Dulbelcco's Modified Eagle Medium, DMEM)배지 200㎕에서 24시간 배양한 후, 배지중의 최종 농도가 1mM이 되도록 희석시킨 H2O2를 10㎕ 가하고, 동시에 육계피 추출물의 1,3-부틸렌 글리콜(50v/v, 수용액)용액 최종 농도가 0, 10, 20, 50, 100㎍/㎖가 되도록 가하고 24시간 배양한 후 웰당 MTT 용액 (tetrazolium salt 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide)을 10㎕씩 첨가하고 4시간 방치 후, 배지 부분을 버렸다. 각 웰당 100㎕의 디메틸 설폭시드(DMSO)용액을 가하여 20분간 교반 후 마이크로플레이트 리더(microplate reader)로 570nm에서 흡광도를 측정하였다.Cinnamon extract was prepared in Example 6. Mouse-derived fibroblasts (fibroblast, NIH / 3T3) were inoculated into each well (5 × 10 4 ) in a 96-well plate and incubated in 200 μl of Dulbelcco's Modified Eagle Medium (DMEM) medium for 24 hours. 10 μl of H 2 O 2 diluted to a final concentration of 1 mM in the medium was added, and at the same time, the final concentration of the 1,3-butylene glycol (50v / v, aqueous solution) solution of the cinnamon extract was 0, 10, 20, 50 , Add 100 ㎍ / ㎖, incubate for 24 hours, and add 10 μl of MTT solution (tetrazolium salt 3- (4,5-Dimethylthiazol-2-yl) -2,5-diphenyl tetrazolium bromide) per well and leave for 4 hours. After that, the medium portion was discarded. 100 μl of dimethyl sulfoxide (DMSO) solution was added to each well, followed by stirring for 20 minutes, and the absorbance was measured at 570 nm using a microplate reader.
2) 실험 결과2) Experiment result
육계피 추출물의 과산화 수소(H2O2) 유도 세포독성에 대한 억제 효과를 표 4 나타내었다. 세포 독성 억제효과는 다음 공식에 의해 산출하였다.Table 4 shows the inhibitory effect on the hydrogen peroxide (H 2 O 2 ) -induced cytotoxicity of the cinnamon extract. The cytotoxic effect was calculated by the following formula.
세포독성 억제 효과()= [(추출물 처리시의 흡광도 - H2O2처리시의 흡광도) /H2O2처리시의 흡광도] × 100Cytotoxic inhibitory effect () = [(absorbance at extract treatment-absorbance at H 2 O 2 treatment) / absorbance at H 2 O 2 treatment] × 100
* 상기 값은 3회 실시 평균값임* The above value is the average of 3 runs
상기의 결과에서 나타난 것처럼 육계피 추출물은 과산화수소(H2O2) 유도 세포독성에 대한 억제를 농도 의존적으로 나타내었다. 따라서 육계피 추출물은 섬유아세포내에서 생성된 히드록실 라디칼을 소거하여 세포 독성을 억제하고 있는 것으로 나타났다.As shown in the results, the cinnamon extract showed a concentration-dependent inhibition of hydrogen peroxide (H 2 O 2 ) -induced cytotoxicity. Therefore, cinnamon extract was found to suppress cytotoxicity by scavenging hydroxyl radicals generated in fibroblasts.
실험예 3Experimental Example 3
육계피 추출물의 세포 독성Cytotoxicity of Cinnamon Extract
육계피 추출물 자체의 세포 독성을 마우스 유래 피부 섬유아세포(fibroblast, NIH/3T3)를 사용하여 측정하였다.Cytotoxicity of the cinnamon extract itself was measured using mouse-derived skin fibroblasts (NIH / 3T3).
1) 실험 방법1) Experiment Method
육계피 추출물을 실시예 6에서 제조한 것을 사용하였다.The cinnamon extract prepared in Example 6 was used.
마우스 유래 섬유아세포를 웰당 5×104개를 96웰 플레이트에 접종하여 디엠이엠(DMEM) 배지 200㎕에서 배양한 후 육계피 추출물 용액 최종 농도가 0, 50, 100, 200, 500㎍/㎖이 되도록 가하고 24시간 배양하였다. MTT용액을 10㎕씩 첨가하고 4시간 방치 후, 디메틸 설폭시드(DMSO)를 웰당 100㎕씩 가하여 20분간 교반하면서 용해시킨 다음 마이크로플레이트 리더(Microplate reader)로 570nm에서 흡광도를 측정하였다.Mouse-derived fibroblasts were inoculated with 5 × 10 4 cells per well into a 96-well plate and cultured in 200 μl of DMEM medium. The final concentration of the cinnamon extract solution was 0, 50, 100, 200, 500 μg / ml. Added and incubated for 24 hours. After 10 μl of MTT solution was added and left for 4 hours, 100 μl of dimethyl sulfoxide (DMSO) was added to the wells, dissolved for 20 minutes, and the absorbance was measured at 570 nm using a microplate reader.
2)실험 결과2) Result of experiment
육계피 추출물 자체의 세포 독성을 표 5에 나타내었다. 세포 독성은 세포 생존율로서 하기의 공식에 따라 산출하였다.The cytotoxicity of the cinnamon extract itself is shown in Table 5. Cytotoxicity was calculated according to the following formula as cell viability.
세포생존율()= (추출물 처리시의 흡광도/대조군의 흡광도) × 100Cell viability () = (absorbance at extract treatment / absorbance of control) × 100
* 상기 값은 3회 실시 평균값임* The above value is the average of 3 runs
처방예 1Prescription Example 1
육계피 추출물을 함유한 화장료 중 유연 화장수의 처방예는 다음과 같다.A prescription example of the flexible lotion in the cosmetic containing the extract of cinnamon is as follows.
처방예 2Prescription Example 2
육계피 추출물을 함유한 화장료 중 밀크 로션의 처방예는 다음과 같다.A prescription example of milk lotion among cosmetics containing cinnamon extract is as follows.
처방예 3Prescription Example 3
육계피 추출물을 함유한 화장료 중 영양 크림의 처방예는 다음과 같다.A prescription example of a nourishing cream in cosmetics containing a cinnamon extract is as follows.
처방예 4Prescription Example 4
육계피 추출물을 함유한 화장료 중 에센스의 처방예는 다음과 같다.Prescription example of the essence of cosmetics containing the extract of cinnamon is as follows.
처방예 5Prescription Example 5
육계피 추출물을 함유한 화장료 중 맛사지 크림의 처방예는 다음과 같다.A prescription example of a massage cream in cosmetics containing a cinnamon extract is as follows.
처방예 6Prescription Example 6
육계피 추출물을 함유한 화장료 중 팩 처방예는 다음과 같다.Examples of pack formulation among cosmetics containing the extract of cinnamon are as follows.
상기에서 알 수 있는 바와 같이, 본 발명에 따른 육계피 추출물은 매우 우수한 자유라디칼의 소거효과가 있을 뿐 아니라, 세포독성이 거의 없어 안전성이 우수하므로 화장료 조성물에 효과적으로 사용될 수 있다.As can be seen from the above, the cinnamon extract according to the present invention not only has a very good free radical scavenging effect, but also has little cytotoxicity, and thus can be effectively used in cosmetic compositions.
Claims (7)
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| Application Number | Priority Date | Filing Date | Title |
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| KR1019990034707A KR20010018668A (en) | 1999-08-20 | 1999-08-20 | A cosmetic composition containing Cinnamomi Cortex extracts |
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| KR1019990034707A KR20010018668A (en) | 1999-08-20 | 1999-08-20 | A cosmetic composition containing Cinnamomi Cortex extracts |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100787634B1 (en) * | 2005-12-19 | 2007-12-21 | 한불화장품주식회사 | Cosmetic composition having antiseptic activity |
| US20120070517A1 (en) * | 2009-06-04 | 2012-03-22 | Jin Auh | Pharmaceutical composition containing herbal extract for prevention or treatment of nephritis |
| KR20160011865A (en) * | 2014-07-23 | 2016-02-02 | 현대자동차주식회사 | Apparatus and Method for controlling a throttle valve |
| US10098921B2 (en) | 2014-12-23 | 2018-10-16 | Korea Institute Of Oriental Medicine | Method for treating gout and gout-induced arthritis using composition containing mixed extract of Chrysanthemum indicum and Cinnamomum cassia |
| KR20190088207A (en) * | 2018-01-18 | 2019-07-26 | 경희대학교 산학협력단 | Composition for treating hair loss and promoting hair growth |
| KR20210096733A (en) * | 2020-01-29 | 2021-08-06 | 한남대학교 산학협력단 | A shampoo composition for preventing hair loss using nanocapsule |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS57163307A (en) * | 1981-03-30 | 1982-10-07 | Pola Chem Ind Inc | Cosmetic |
| JPH045237A (en) * | 1990-04-24 | 1992-01-09 | Nonogawa Shoji Kk | Superoxide eliminant |
| JPH11106311A (en) * | 1997-07-31 | 1999-04-20 | Sansho Seiyaku Co Ltd | Hyaluronidase activity inhibitor and its use |
| JPH11171758A (en) * | 1997-12-12 | 1999-06-29 | Maruzen Seiyaku Kk | Elastase inhibitor and preparation for external use for skin |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS57163307A (en) * | 1981-03-30 | 1982-10-07 | Pola Chem Ind Inc | Cosmetic |
| JPH045237A (en) * | 1990-04-24 | 1992-01-09 | Nonogawa Shoji Kk | Superoxide eliminant |
| JPH11106311A (en) * | 1997-07-31 | 1999-04-20 | Sansho Seiyaku Co Ltd | Hyaluronidase activity inhibitor and its use |
| JPH11171758A (en) * | 1997-12-12 | 1999-06-29 | Maruzen Seiyaku Kk | Elastase inhibitor and preparation for external use for skin |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100787634B1 (en) * | 2005-12-19 | 2007-12-21 | 한불화장품주식회사 | Cosmetic composition having antiseptic activity |
| US20120070517A1 (en) * | 2009-06-04 | 2012-03-22 | Jin Auh | Pharmaceutical composition containing herbal extract for prevention or treatment of nephritis |
| US8642096B2 (en) * | 2009-06-04 | 2014-02-04 | Ahn-Gook Pharmaceutical Co., Ltd. | Pharmaceutical composition containing herbal extract for prevention or treatment of nephritis |
| KR20160011865A (en) * | 2014-07-23 | 2016-02-02 | 현대자동차주식회사 | Apparatus and Method for controlling a throttle valve |
| US10098921B2 (en) | 2014-12-23 | 2018-10-16 | Korea Institute Of Oriental Medicine | Method for treating gout and gout-induced arthritis using composition containing mixed extract of Chrysanthemum indicum and Cinnamomum cassia |
| KR20190088207A (en) * | 2018-01-18 | 2019-07-26 | 경희대학교 산학협력단 | Composition for treating hair loss and promoting hair growth |
| KR20210096733A (en) * | 2020-01-29 | 2021-08-06 | 한남대학교 산학협력단 | A shampoo composition for preventing hair loss using nanocapsule |
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