KR20010008993A - Functional beverage containing submerged culture broth of Paecilomyces sp. and process for preparation thereof - Google Patents
Functional beverage containing submerged culture broth of Paecilomyces sp. and process for preparation thereof Download PDFInfo
- Publication number
- KR20010008993A KR20010008993A KR1019990027106A KR19990027106A KR20010008993A KR 20010008993 A KR20010008993 A KR 20010008993A KR 1019990027106 A KR1019990027106 A KR 1019990027106A KR 19990027106 A KR19990027106 A KR 19990027106A KR 20010008993 A KR20010008993 A KR 20010008993A
- Authority
- KR
- South Korea
- Prior art keywords
- liquid culture
- snow
- drink
- mycelia
- added
- Prior art date
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/38—Other non-alcoholic beverages
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
- A23V2200/324—Foods, ingredients or supplements having a functional effect on health having an effect on the immune system
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
- A23V2200/326—Foods, ingredients or supplements having a functional effect on health having effect on cardiovascular health
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
- A23V2200/33—High-energy foods and drinks, sports drinks
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2250/00—Food ingredients
- A23V2250/20—Natural extracts
- A23V2250/208—Fungi extracts
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2300/00—Processes
- A23V2300/50—Concentrating, enriching or enhancing in functional factors
Landscapes
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Non-Alcoholic Beverages (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
Description
본 발명은 눈꽃동충하초(Paecilomyces sp.) 액체배양물을 함유한 기능성 음료 및 그 제조방법에 관한 것이다. 더욱 상세하게는, 눈꽃동충하초를 액체배양하여 얻은 균사체와 고분자다당체를 각종 식품첨가제와 함께 적당량 배합한 인체 생리활성 증강용 기능성 음료 및 그 제조방법에 관한 것이다.The present invention relates to a functional beverage containing Paecilomyces sp. Liquid culture and a method for producing the same. More specifically, the present invention relates to a functional drink for enhancing the human physiological activity, and a method for producing the same, in which an appropriate amount of the mycelium and the polymer polysaccharide obtained by liquid culture of the snow whim fungus is mixed with various food additives.
동충하초 균사체는 여러 가지 생리활성 기능이 입증되면서 부터 한약제제로 이용되어 왔다. 그러나 동충하초를 대량으로 배양하여 이러한 의약적 기능성을 광범위하게 이용하기 위해서는 대량 배양기술이 필요한데, 주로 균사체 고체배양기술에 관한 연구가 활발하게 진행되어 왔다.Cordyceps mycelia have been used as herbal medicines since various bioactive functions have been demonstrated. However, in order to cultivate Cordyceps sinensis in a large amount and to use such medicinal functions extensively, mass culture technology is required, and research on mycelium solid culture technology has been actively conducted.
본 발명자들은 눈꽃동충하초 균사체를 액체배양할 경우, 배양기간중에 생리활성 기능이 우수한 고분자다당체가 생산되고, 이 물질을 동충하초 균사체와 함께 이용할 경우 균사체 또는 고분자다당체 단독으로 사용하는 경우에 버금가는 인체 생리활성 증강효과를 발견하고 기능성 음료를 제조하므로써 본 발명을 완성하였다. 따라서 본 발명의 목적은 액체배양하여 얻은 눈꽃동충하초 균사체와 고분자 다당체를 함유하는 인체 생리활성 증강용 기능성 음료를 제공함에 있다. 본 발명의 다른 목적은 상기 인체 생리활성 증강용 기능성 음료의 제조방법을 제공함에 있다.The inventors of the present invention, when the liquid mycelial mycelia, liquid polysaccharides with excellent physiological activity during the cultivation period is produced, and when using the same with the Cordyceps mycelium mycelium body or biomolecular activity equivalent to the case of using the mycelium or polysaccharide alone The present invention has been completed by discovering the enhancing effect and preparing the functional beverage. Therefore, an object of the present invention is to provide a functional drink for enhancing the human body's physiological activity, which contains the snow fungus mycelia and polymer polysaccharide obtained by liquid culture. Another object of the present invention to provide a method for producing a functional drink for enhancing the human body physiological activity.
본 발명의 상기 목적은 눈꽃동충하초를 최적 조건에서 액체배양하여 균사체와 고분자다당체를 대량 생산하고 생산한 균사체와 고분자다당체를 감미제, 산미제, 생약제제, 비타민류 및 무기염 등과 적당히 배합하여 기능성 음료를 제조한 후 이 기능성 음료의 면역활성, 혈중콜레스테롤 저하효과, 혈당저하효과 및 운동력 증강효과를 조사하므로써 달성하였다.The above object of the present invention is to mass-produce mycelium and polymer polysaccharides by liquid culture of the snow whim fungus in optimum conditions and to produce a functional drink by properly blending the produced mycelium and polymer polysaccharide with sweeteners, acidulants, herbal preparations, vitamins and inorganic salts. After preparation, this function was achieved by investigating the immune activity, blood cholesterol lowering effect, hypoglycemic effect and exercise enhancing effect.
이하 본 발명의 구성 및 작용을 설명한다.Hereinafter, the configuration and operation of the present invention.
도 1은 본 발명 눈꽃동충하초 액체배양물 함유 기능성 음료에 첨가되는 눈꽃동충하초 액체배양 과정을 나타낸다.Figure 1 shows the process of growing a snow cell Cordyceps liquefied liquid added to the functional beverage containing snowflake Cordyceps liquefied invention of the present invention.
본 발명은 경북농촌진흥원에서 분양 받은 눈꽃동충하초를 합성배지, 폐당밀, 옥수수 추출잔류물(corn steep liquor), 전분가수분해물(시럽), 감자추출물, 설탕 등의 식용가능한 산업용 배지를 사용하여 균사체 및 고분자다당체를 최대수율로 생산하는 액체배양 단계; 상기 균사체 및 고분자다당체를 감미제, 산미제, 생약제제, 비타민류 및 염, 아미노산, 무기물, 철염류, 식이성 섬유, 천연과즙, 착향제, 안정화제, 카페인, 보존제 및 영양원 등과 혼합하여 기능성 음료를 제조하는 단계; 제조한 상기 기능성음료의 인체 면역증강, 혈당 및 혈중지질 강하, 항고혈압, 운동력증강 등의 효과를 조사하는 단계로 구성된다.The present invention is a mycelium and the mycelia by using an edible industrial medium, such as synthetic medium, waste molasses, corn steep liquor, starch hydrolyzate (syrup), potato extract, sugar, etc. Liquid culture step of producing a polymer polysaccharide in maximum yield; Functional beverages are mixed with the mycelium and polysaccharides with sweeteners, acidulants, herbal preparations, vitamins and salts, amino acids, minerals, iron salts, dietary fiber, natural juices, flavoring agents, stabilizers, caffeine, preservatives and nutrients. Manufacturing step; Comprising the steps of investigating the effects of the human immune enhancement, blood glucose and blood lipid lowering, antihypertensive, exercise strength of the functional beverage prepared.
본 발명에서 사용한 균주는 경북농촌진흥원에서 분양 받았으며, 4℃에서 매 2개월마다 계대배양하여 보존하였다.The strain used in the present invention was distributed at Gyeongbuk Rural Development Administration, and preserved by subcultured every 2 months at 4 ° C.
본 발명에서 비타민류는 니코틴산아마이드, 비타민 A, B, C, D, E, 엽산을 단독 또는 2종 이상 배합하여 사용하였다. 아미노산류는 L-글리신, L-라이신, DL-메치오닌, L-발린, 비오틴, L-시스테인, L-시스틴, L-트립토판, L-트레오닌, L-페닐알라닌을 단독 또는 2종 이상 배합하여 사용하였다. 무기물은 칼슘염으로 구연산칼슘, 탄산칼슘, 판토텐산칼슘, 글루콘산칼슘, 식물성칼슘, 동물성칼슘 등을 단독 또는 2종 이상 배합하여 사용하였다. 철염류는 젖산철, 구연산철을 단독 또는 2종 이상 배합하여 사용하였다. 영양원은 벌꿀 또는 로얄제리를 사용하였다. 식이성 섬유는 알로에, 치커리 등의 각종 유용식물의 추출물, 잔탄검 등의 각종 검류, 덱스트린, 용해성 섬유 등을 단독 또는 2종 이상 배합하여 사용하였다. 생약제제는 구기자, 오가피, 백출, 백작약, 녹곽, 홍삼, 인삼, 음양곽, 우황, 황정을 단독 또는 2종 이상 배합하여 사용하였다. 천연과즙류는 파인애플, 레몬, 감귤, 오렌지, 사과, 배, 그레이프후르츠, 살구, 딸기, 복숭아, 멜론, 구아바, 레몬, 자두를 단독 또는 2종 이상 배합하여 사용하였다. 착향제는 혼합과일향, 사과향, 요구르트향, 드링크향을 단독 또는 2종 이상 배합하여 사용하였다. 감미제류는 정백당, 포도당, 과당, 프락토올리고당, 이소말토올리고당, 말토올리고당, 키토산올리고당, 키토올리고당, 대두올리고당, 자일로올리고당, 이성화당(고과당), 전화당, 아스파탐, 스테비오사이드, 솔비톨, 만니톨 중 단독 또는 2종 이상 배합하여 사용하였다. 산미제는 구연산, DL-사과산, 호박산을 단독 또는 2종 이상 배합하여 사용하였다. 안정화제는 소르베이트류 중 폴리소르베이트 20, 폴리소르베이트 40, 폴리소르베이트 80을 단독 또는 2종 이상 배합하여 사용하였고 보존제류는 안식향산 및 그의 유도체, 데히드로초산 및 그의 염류, 소르빈산 및 그의 염류을 단독 또는 2종 이상 배합하여 사용하였다.In the present invention, vitamins were used alone or in combination of two or more kinds of nicotinic acid amide, vitamins A, B, C, D, E, folic acid. As amino acids, L-glycine, L-lysine, DL-methionine, L-valine, biotin, L-cysteine, L-cystine, L-tryptophan, L-threonine, and L-phenylalanine were used alone or in combination. . As the inorganic salt, calcium citrate, calcium carbonate, calcium pantothenate, calcium gluconate, vegetable calcium, animal calcium, and the like were used alone or in combination of two or more thereof. Iron salts were used alone or in combination of two or more kinds of iron lactate and iron citrate. Nutritional sources used honey or royal jelly. The dietary fiber was used alone or in combination of two or more kinds of extracts of various useful plants such as aloe and chicory, various gums such as xanthan gum, dextrin, soluble fiber and the like. The herbal preparations were used alone or in combination of two or more of Gugija, Ogapi, Baekchul, Baekjak, Green, Red Ginseng, Ginseng, Yin-yang Kwak, Uhwang, Hwangjeong. Natural juices were used alone or in combination of pineapple, lemon, citrus, orange, apple, pear, grapefruit, apricot, strawberry, peach, melon, guava, lemon, plum. Flavoring agents were used alone or in combination of two or more mixed fruit flavor, apple flavor, yogurt flavor, drink flavor. Sweeteners include white sugar, glucose, fructose, fructooligosaccharide, isomaltooligosaccharide, malto oligosaccharide, chitosan oligosaccharide, chito oligosaccharide, soy oligosaccharide, xylo oligosaccharide, isosugar (high fructose), invert sugar, aspartame, stevioside, sorbitol, It was used individually or in mixture of 2 or more types of mannitol. Acidifiers were used alone or in combination of two or more citric acid, DL- apple acid, succinic acid. Stabilizers were used alone or in combination of two or more polysorbate 20, polysorbate 40, polysorbate 80 in the sorbate, and preservatives are benzoic acid and its derivatives, dehydroacetic acid and its salts, sorbic acid and salts thereof It used individually or in mixture of 2 or more types.
이하 본 발명의 구체적인 방법을 실시예를 들어 상세히 설명하고자 하지만 본 발명의 권리범위는 이들 실시예에만 한정되는 것은 아니다.Hereinafter, the specific method of the present invention will be described in detail with reference to Examples, but the scope of the present invention is not limited only to these Examples.
실시예 1: 눈꽃동충하초 액체배양에 의한 균사체 및 고분자다당체의 생산Example 1: Production of Mycelia and Polysaccharides by Liquid Culture of Snow Cordyceps
본 실시예에서는 균사체성장 및 고분자다당체 생산을 위해 갈락토스 1g/L, 설탕 9g/L, 자일로스 1g/L, 포도당 9g/L, 효모추출물 0.5g/L, 감자전분배지 2g/L, NH4H2PO40.5g/L, DL-세린 0.5g/L, KH2PO41g/L, CaCl20.6g/L, MgSO4·7H2O, MnSO4·5H2O 및 FeSO4·7H2O 각각 0.02g/L, 티아민염산 0.001g/L으로 조성된 초기 pH 4.5인 합성배지; 폐당밀 1~10 % W/V, 콘스팁즙(corn steep liquor) 1~10 % W/V를 함유하는 식용이 가능한 산업용 배지; 전분가수분해물(시럽) 1~10 % W/V; 감자추출물 1~10 % W/V; 설탕 1~10 % W/V을 단독 또는 2종 이상 배합한 배지에서 눈꽃동충하초 균사체를 액체배양한 후 이 액체배양액을 교반배양조상의 배지 200 mL를 함유하는 500 mL 플라스크에 담아 통기속도 200mL/분, 온도 20℃ 및 pH 6.0인 5리터 발효기내로 무균적으로 접종하고 상기와 동일한 조건에서 12일 동안 배양하였다. 배양물을 10447g로 20분 동안 원심분리하여 균사체 14 g/L을 얻었고 상등액을 다시 에탄올 침전(에탄올: 배양액=4:1)시킨 후 다시 10447g로 20분간 원심분리하여 상등액을 제외한 고분자다당체 6 g/L을 얻었다.In this example, 1 g / L of galactose, 9 g / L of sugar, 1 g / L of glucose, 9 g / L of glucose, 0.5 g / L of yeast extract, 2 g / L of potato starch medium, NH 4 H for mycelial growth and polysaccharide production 2 PO 4 0.5 g / L, DL-serine 0.5 g / L, KH 2 PO 4 1 g / L, CaCl 2 0.6 g / L, MgSO 4 7H 2 O, MnSO 4 5H 2 O and FeSO 4 7H 2 A synthetic medium having an initial pH of 4.5 consisting of 0.02 g / L each and 0.001 g / L thiamine hydrochloride; Edible industrial medium containing 1-10% W / V of molasses, corn steep liquor 1-10% W / V; Starch hydrolyzate (syrup) 1-10% W / V; Potato extract 1-10% W / V; A liquid culture of Snow Cordyceps fungus mycelium in a medium containing 1-10% W / V of sugar alone or in a mixture of two or more kinds, and then the liquid culture solution was placed in a 500 mL flask containing 200 mL of medium in a stirred culture vessel, and the aeration rate was 200 mL / min. , Aseptically inoculated into a 5 liter fermenter with a temperature of 20 ° C. and pH 6.0 and incubated for 12 days under the same conditions as above. The culture was centrifuged at 10447 g for 20 minutes to obtain 14 g / L of mycelium. The supernatant was ethanol precipitated again (ethanol: culture solution = 4: 1), and then centrifuged at 10447 g for 20 minutes to give 6 g / of polysaccharide except the supernatant. L was obtained.
실시예 2: 눈꽃동충하초 균사체와 고분자다당체 함유 기능성 음료의 제조Example 2: Preparation of functional beverage containing snow Cordyceps mycelia and polymer polysaccharide
상기 실시예 1에서 얻은 눈꽃동충하초 균사체와 고분자다당체 모두를 포함하는 눈꽃동충하초 액체배양액을 열정제수 또는 막여과수 1L에 1 ~ 100 g을 녹인 후 식품첨가물로 니코틴산아마이드 0.001 ~ 5중량%, 엽산 0.01 ~ 5중량%, 아스파라긴산 0.01 ~ 5중량%, 구연산 칼슘 0.01 ~ 1.0중량%, 벌꿀 0.01 ~ 3중량%, 덱스트린 0.01 ~ 3중량%, 인삼엑기스 0.01 ~ 10중량%, 사과 0.01 ~ 5중량%, 사과향 0.05 ~ 1중량%, 프락토올리고당 0.1 ~ 10중량%, 구연산 0.05 ~ 5중량%, 폴리소르베이트 0.01 ~ 3.0중량%, 안식향산 0.05 ~ 1중량%을 첨가하여 식품첨가물이 전체 조성비율에 0.28 ~ 55중량% 범위가 되도록 하였다. 이때, 눈꽃동충하초 균사체와 고분자다당체는 전체 조성비율에 대해 0.01 ~ 30중량%이고 바람직하게는 0.1 ~ 10중량%이었다.After dissolving 1 ~ 100 g of Snow Cultivated Mycorrhizal Mycelia and polymer polysaccharide obtained in Example 1 in 1L of Passion Water or Membrane Filtrate, 0.001-5 wt% of Niacinamide, Folic Acid 0.01-5 Weight%, Aspartic acid 0.01 to 5%, Citrate 0.01 to 1.0%, Honey 0.01 to 3%, Dextrin 0.01 to 3%, Ginseng extract 0.01 to 10%, Apple 0.01 to 5%, Apple flavor 0.05 to 1% by weight, fructooligosaccharide 0.1 to 10% by weight, citric acid 0.05 to 5% by weight, polysorbate 0.01 to 3.0% by weight, benzoic acid 0.05 to 1% by weight, food additives 0.28 ~ 55% by weight Range. At this time, the Snow Cordyceps mycelia and the polymer polysaccharide were 0.01 to 30% by weight and preferably 0.1 to 10% by weight relative to the total composition ratio.
실시예 3: 눈꽃동충하초 균사체 함유의 기능성 음료 제조Example 3 Preparation of Functional Drink Containing Snow Cordyceps Mycelia
실시예 1에서 눈꽃동충하초를 액체 배양하여 얻은 균사체만을 함유하는 기능성 음료를 실시예 2와 동일한 방법에 의해 제조하였다. 즉, 실시예 1에서 눈꽃동충하초 액체 배양물을 10447g로 20분 동안 원심분리하여 얻은 균사체를 열정제수 또는 막여과수 1L에 1 ~ 100 g을 녹인 후 식품첨가물로 니코틴산아마이드, 엽산, 아스파라긴산, 구연산 칼슘, 벌꿀, 덱스트린, 인삼, 사과, 사과향, 프락토올리고당, 구연산, 폴리소르베이트, 안식향산을 상기 실시예 2와 동일한 양으로 첨가하였다. 이때, 눈꽃동충하초 균사체는 실시예 2와 동일하게 전체 조성비율에 대해 0.01 ~ 30중량%이고 바람직하게는 0.1 ~ 10중량%이었다.In Example 1, a functional beverage containing only mycelia obtained by liquid cultivation of Snow Clover, was prepared in the same manner as in Example 2. That is, in Example 1, 1 to 100 g of the mycelia obtained by centrifuging the snow fungus Cultivated liquid culture for 10 minutes at 10447 g was dissolved in 1 L of Passion or Membrane Fructus, and as a food additive, nicotinic acid amide, folic acid, aspartic acid, calcium citrate, Honey, dextrin, ginseng, apple, apple flavor, fructooligosaccharide, citric acid, polysorbate, benzoic acid were added in the same amount as in Example 2. At this time, the snow Cordyceps sinensis mycelium was 0.01 to 30% by weight and preferably 0.1 to 10% by weight relative to the total composition ratio as in Example 2.
실시예 4: 눈꽃동충하초 고분자다당체 함유의 기능성 음료의 제조Example 4 Preparation of Functional Beverage Containing Snowmobile Cordyceps Polysaccharide
실시예 1에서 눈꽃동충하초 액체배양물을 원심분리한 후 그 상등액을 다시 원심분리하여 얻은 고분자다당체를 함유하는 기능성 음료를 실시예 2와 동일한 방법에 의해 제조하였다. 즉, 실시예 1에서 눈꽃동충하초 액체 배양물을 10447g로 20분 동안 원심분리하여 얻은 상등액을 다시 에탄올 침전(에탄올: 배양액=4:1)시킨 후 10447g로 20분간 원심분리하여 얻은 고분자다당체를 열정제수 또는 막여과수 1L에 1 ~ 100 g을 녹인 후 식품첨가물로 니코틴산아마이드, 엽산, 아스파라긴산, 구연산 칼슘, 벌꿀, 덱스트린, 인삼, 사과, 사과향, 프락토올리고당, 구연산, 폴리소르베이트, 안식향산을 실시예 2와 동일한 양으로 첨가하였다. 이때, 눈꽃동충하초 고분자다당체는 실시예 2와 동일하게 전체 조성비율에 대해 0.01 ~ 30중량%이며 바람직하게는 0.1 ~ 10중량%이었다.In Example 1, a functional beverage containing the polymer polysaccharide obtained by centrifuging the Snow Caterpillar vinegar liquid culture and centrifugation of the supernatant was prepared in the same manner as in Example 2. In other words, in Example 1, the supernatant obtained by centrifuging the Snow Cultivated Cultivated Liquid culture for 20 minutes at 10447 g was subjected to ethanol precipitation (ethanol: culture solution = 4: 1), and then the polymer polysaccharide obtained by centrifugation at 10447 g for 20 minutes was used. Or melt 1 to 100 g in 1 L membrane filtration water and then add nicotinic acid amide, folic acid, aspartic acid, calcium citrate, honey, dextrin, ginseng, apple, apple flavor, fructooligosaccharide, citric acid, polysorbate, benzoic acid as food additives Example 2 It was added in the same amount as. At this time, the snow Cordyceps sinensis polysaccharide is 0.01 to 30% by weight and preferably 0.1 to 10% by weight relative to the total composition ratio as in Example 2.
실시예 5: 눈꽃동충하초균사체와 고분자다당체 함유 음료의 기능성평가Example 5: Functional Evaluation of Snow Cordyceps Mycelia and Polymer Polysaccharide-Containing Beverages
본 실시예에서는 실시예 2에서 제조한 눈꽃동충하초 균사체와 고분자다당체 모두 함유하는 기능성 음료의 주요 기능성을 평가하였다. 기능성평가로 면역활성 측정은 항보체활성 측정방법인 Mayer법으로 측정하였다. 즉, NHS (normal human serum), GVB++(gelatin veronal buffer saline pH 7.2) 와 시료를 각각 50㎕씩 혼합하여 37℃에서 30분간 반응시킨 후, 반응액에 GVB++를 350㎕씩 첨가하고 이를 10배에서 160배까지 연속희석하였다. 여기에 750㎕의 GVB++와 양의 감작혈구 (IgM haemolysin sensitized sheep erythrocyte, 108cell/mL)를 250mL씩 가하여 1시간 동안 반응시킨 후, PBS (phosphate buffer saline, pH7.4)를 2.5mL씩 가하여 원심분리한 후 상등액의 흡광도를 412nm에서 측정하였다. 항보체 활성은 ITCH50, 즉 총보체 용혈 저지율 (inhibition of 50% total complement hemolysis)로 나타냈다. 시료의 정확한 활성을 검증하기 위해 역가를 알고있는 항보체다당 CAP-O (ITCH50= 80%) 을 표준물로 계산하였다. 계산방법은 하기와 같다.In this example, the main functionality of the functional beverage containing both the Snow Cordyceps mycelia and the polymer polysaccharide prepared in Example 2 was evaluated. Immune activity was measured by the Mayer method. That is, 50 μl of NHS (normal human serum), GVB ++ (gelatin veronal buffer saline pH 7.2) and the sample were mixed and reacted at 37 ° C. for 30 minutes, and then 350 μl of GVB ++ was added to the reaction solution. This was serially diluted from 10 to 160 times. 250 mL of 750 μl GVB ++ and positive sensitized blood cells (IgM haemolysin sensitized sheep erythrocyte, 10 8 cell / mL) were added thereto and reacted for 1 hour, followed by 2.5 mL of PBS (phosphate buffer saline, pH7.4). After centrifugation, the absorbance of the supernatant was measured at 412 nm. Anti-complement activity was indicated by ITCH 50 , the inhibition of 50% total complement hemolysis. In order to verify the exact activity of the sample, anti-complementary polysaccharide CAP-O (ITCH 50 = 80%) with known titer was calculated as a standard. The calculation method is as follows.
혈중콜레스테롤 저하효과 측정은 지방을 과량 섭취시켜 고지혈증이 유발된 흰쥐에 2주간 샘플을 경구투여한 후, 실험동물을 12시간동안 절식시켜 에테르로 마취하고 개복하여 간조직을 회수하고, 혈액을 복부 동맥에서 채취하였다. 혈액은 원심분리하여 혈장 분리 후 실험에 사용하였다. 총 콜레스테롤 함량은 효소법을 이용한 키트(AM 202-K)를 사용하여 측정하였다. 혈당저하효과 측정은 5주령의 웅성 흰쥐를 구입하여 1 주간 고형 사료(삼양사료)로 적응시킨 후 사육상에 한 마리씩 넣어 4주간 AIN-76 식이로 사육하였다. 사육실은 온도 20℃, 습도 50%를 유지하며 12시간 간격으로 점등 및 소등하였다. 물은 증류수를 사용하였으며 증류수 및 식이는 자의대로 섭취하도록하며 실험 기간동안 식이 섭취량은 2일에 한 번씩, 체중 증가량은 3일에 한 번씩 측정하였다. 흰쥐는 각 군당 10 마리씩의 편성하며 먼저 평균 체중이 비슷한 것끼리 분류한 후 각군의 평균 체중이 비슷할 수 있도록 무작위로 분류하였다. 당뇨의 유발은 췌장의 β-cell 만을 특이적으로 파괴하여 인슐린 분비량을 낮춤으로써 고혈당을 일으킨다고 알려진 스트렙토조토신(streptozotocin;STZ )(Sigma chemical Co., 50mg/kg BW/0.01M citrate buffer, pH 4.5)을 1회 실험동물에 근육주사하여 실험적으로 IDDM 형태의 당뇨를 유발시켰다. STZ 주사 후 24시간 동안 절식시킨 뒤 뇨당을 측정하여 당량이 300mg/dl 이상인 것 만을 당뇨가 유발된 것으로 판정하여 사용하였다. STZ로 당뇨 유발이 판명된 흰쥐에 시료을 체중(kg)당 50∼100㎎의 투여로 2주간 매일 경구 투여하며, 식이를 섭취하기전 공복시에 행함으로써 시료 섭취 효율을 증가시켰다. 당뇨를 유발시키지 않은 정상 대조군은 당뇨 유발군과 동일한 스트레스를 주기 위해 같은 양의 생리 식염수를 주사하였다. 마찬가지로 시료를 경구투여하지 않는 당뇨 유발 대조군과 정상 대조군 모두에게 생리 식염수를 경구 투여하여 동일한 스트레스를 주었다. 2주간 시료를 경구투여한 뒤 실험동물을 12시간동안 절식시킨 뒤 에테르로 마취시킨 다음 개복하여 복부 동맥에서 혈액을 채취하였다. 혈중 당의 농도는 글루코스로 부터 글루코스 옥시다제의 작용에 의해 과산화 수소의 발생을 원리로 이용한 글루코스 옥시다제 법에 따라 조제된 일본의 Eiken사의 kit(GLZYME)을 이용하여 측정하였다. 운동력 증강효과 측정은 수영 한계측정방법에 의하였다. 마우스의 수영장치는 내부 90×45×45㎝(L×W×H)의 아크릴제품으로 수영장의 밑바닥에서 38㎝의 높이까지 물을 채우고, 펌프(C-P60H, 日立製作所, 日本)와 유량계(FC-A20, 日本 東京 Hitachi 연구소)을 장착하여 사용하였다. 수온은 수영장의 밑바닥에 설치한 가열기에 의해 34℃로 조절하였다. 물의 유출구는 비닐제품의 파이프에 정밀하게 일직선상에 구멍을 뚫고, 물 전체에 영향을 주도록 만들었다. 그리고 파이프를 물 표면에서 2∼3㎝ 아래에 설치하여 물의 흐름 방향을 일정하게 유지하도록 하며, 물의 유속은 수차형유속계(SPC-형, 日本 三光精密工業)로 수영장 표면에서 유속을 측정하였다. 실험 기간동안 수영장의 유량은 8L/min (pool 중앙부의 표면유속은 약 166㎝/sec)으로 설정하였다. 마우스를 수영장에 입수시켜 수영을 개시하였으며 계속된 수영으로 마우스가 피로해지면 수영장 후부로 가는 것을 확인하였다. 따라서 수영장후부에 판을 경사지게 설치하고, 여기에는 물이 밑으로 내려 가도록 설치되어 있어 피로한 마우스는 수영장 후부에서 물의 흐름에 의해 물 속으로 내려가고 마우스가 물 속에서 7초 이상 머무르게 되면 운동능력이 다했다고 판단하여 마우스를 수영장에서 꺼내고 시간을 기록하였다. 이 수영 시간으로 마우스의 운동능력을 나타냈다. 수영이 끝난 마우스는 물기를 닦고, 몸을 말려서 사육 케이스에 넣었다. 그리고 수영 후 휴식기간을 48시간을 주었다가 이와같은 실험을 반복하였다. 실험결과는 표 1에 나타낸 바와 같으며 무첨가음료는 동충하초 액체배양액의 균사체와 고분자다당체를 첨가하지 않은 대조구이며, 첨가음료 A는 동충하초 액체배양액 균사체와 고분자다당체가 100g/L, 첨가음료 B는 75g/L, 첨가음료 C는 50g/L을 첨가한 것이다. 면역활성에 있어서, 첨가음료 A, B, C가 무첨가 음료에 비해 유의적으로 높게 나타났으며 특히 첨가음료 A가 높았다. 혈중콜레스테롤 저하효과도 무첨가 음료에 비해 첨가음료 A, B, C가 우수하였으며 특히 첨가음료 B가 가장 우수하였다. 혈당저하효과도 무첨가 음료에 비해 첨가음료 A, B, C가 우수하였으며 특히 첨가음료 C가 가장 우수하였다. 운동력 증강효과 역시 무첨가 음료보다 첨가음료 A, B, C가 높았으며 특히 첨가음료 A가 높았다.In order to measure the effect of lowering cholesterol in the blood, the rat was ingested with hyperlipidemia for 2 weeks after oral administration of hyperlipidemia, followed by fasting the experimental animals for 12 hours, anesthetizing with ether, and recovering the liver tissue. Was taken from. Blood was centrifuged and used for experiments after plasma separation. Total cholesterol content was measured using the kit using enzyme method (AM 202-K). To measure the hypoglycemic effect, 5 week-old male rats were purchased and adapted to solid feed (Samyang Feed) for 1 week, and then fed to AIN-76 diet for 4 weeks. The breeding room was lit and turned off every 12 hours while maintaining a temperature of 20 ° C. and a humidity of 50%. Distilled water was used for water, and distilled water and diet were taken at will. During the experiment, dietary intake was measured every 2 days and weight gain was measured every 3 days. Rats were divided into 10 groups in each group. First, groups with similar average weights were classified, and then randomly classified so that the average weight of each group was similar. Diabetes induced streptozotocin (STZ) (Sigma chemical Co., 50mg / kg BW / 0.01M citrate buffer, pH), which is known to cause hyperglycemia by specifically destroying β-cells in the pancreas and lowering insulin secretion. 4.5) was injected intramuscularly into experimental animals to experimentally induce diabetes in the form of IDDM. After fasting for 24 hours after STZ injection, urine glucose was measured, and the equivalent weight of 300 mg / dl or more was determined to be diabetes induced. The rats proved to be diabetic with STZ were orally administered to the rats at a dose of 50-100 mg / kg for two weeks, and the sample intake efficiency was increased by fasting before the diet. The normal control group that did not cause diabetes was injected with the same amount of saline to give the same stress as the diabetic group. Likewise, the same stress was given by oral administration of physiological saline to both the diabetic induction control group and the normal control group that did not orally administer the sample. After oral administration of the sample for 2 weeks, the animals were fasted for 12 hours, anesthetized with ether, and then opened, and blood was collected from the abdominal arteries. Blood glucose levels were measured using a Japanese Eiken kit (GLZYME) prepared by the glucose oxidase method using the hydrogen peroxide as a principle by the action of glucose oxidase from glucose. The improvement of exercise power was measured by the swimming limit measurement method. The swimming pool of the mouse is an acrylic product of 90 × 45 × 45 cm (L × W × H), filled with water up to 38 cm from the bottom of the pool, pump (C-P60H, 日本) and flow meter (FC). -A20, Japan Hitachi Institute) was used. The water temperature was adjusted to 34 degreeC by the heater installed in the bottom of a swimming pool. The water outlet made a precise, straight hole in the pipe of the vinyl product, affecting the entire water. And the pipe is installed 2 to 3 cm below the surface of the water to maintain a constant flow direction of water, the flow rate of the water was measured by the aberration type flow meter (SPC-type, Nippon Kogyo Co., Ltd.) at the surface of the pool. During the test period, the flow rate of the pool was set at 8 L / min (surface flow velocity at the center of the pool was about 166 cm / sec). The mice were introduced to the pool to start swimming, and when the mice became tired after the continuous swimming, they were confirmed to go to the rear of the pool. Therefore, the board is inclined at the rear of the swimming pool, and the water is installed downward so that the tired mouse descends into the water by the flow of water at the rear of the swimming pool, and if the mouse stays in the water for more than seven seconds, the athletic ability is achieved. The mouse was taken out of the pool and time was recorded. This swimming time showed the mouse's athletic ability. After swimming, the mice were moistened, dried and placed in a breeding case. After 48 hours of rest and swimming, the experiment was repeated. The experimental results are shown in Table 1, and the additive-free beverage was a control group without added mycelia and polymer polysaccharides of Cordyceps sinensis liquid culture medium, and the added beverage A was 100g / L of Cordyceps liquor liquid culture mycelium and polymer polysaccharide and 75g / L of the beverage B added. L and the added beverage C added 50g / L. In the immunological activity, the beverages A, B, and C were significantly higher than those of the non-added beverages, and in particular, the beverage A was high. The blood cholesterol lowering effect was also superior to the beverages A, B, and C compared to the non-added beverages, especially the beverage B was the best. Hypoglycemic effect was also superior to the beverages A, B, and C compared to the non-added beverages, especially the beverage C was the best. Also, the effect of enhancing the exercise power was higher in the beverages A, B, and C than in the non-added beverages, and in particular, the beverage A was higher.
실시예 6: 눈꽃동충하초균사체 함유 음료의 기능성평가Example 6: Functional evaluation of a beverage containing Snow Cordyceps sinensis mycelia
실시예 3에서 제조한 눈꽃동충하초 액체배양으로부터 생산된 균사체를 함유하는 기능성 음료의 주요 기능성을 상기 실시예 5와 동일한 방법에 의해 평가하였다. 실험결과, 표 2에 나타낸 바와 같으며 여기서 무첨가음료는 눈꽃동충하초 균사체를 첨가하지 않은 대조구이며, 첨가음료 A는 동충하초 균사체 동결건조물 6g/L, 첨가음료 B는 4g/L, 첨가음료 C는 2g/L을 함유시킨 것이다. 표 2에서 보는 바와 같이 면역활성은 무첨가음료에 비해 첨가음료 A, B, C가 모두 높았으며 특히 첨가음료 A가 높았고 혈중콜레스테롤 저하효과, 혈당저하효과 및 운동력 증강효과에서도 모두 무첨가음료에 비해 첨가음료 A, B, C가 우수하였으며 특히 첨가음료 A가 가장 높았다.The main functionality of the functional beverage containing the mycelium produced from the Snow Caterpillar vinegar liquid culture prepared in Example 3 was evaluated by the same method as in Example 5. Experimental results, as shown in Table 2, wherein the additive-free beverage is a control group without adding the snow fungus mycelial, Additive drink A is Cordyceps mycelium lyophilisate 6g / L, Additive drink B is 4g / L, Additive drink C is 2g / It contains L. As shown in Table 2, Immune activity was higher in all of the added beverages A, B, and C compared to the non-added beverages. Especially, the added beverage A was higher, and the blood cholesterol lowering effect, blood sugar lowering effect, and exercise enhancing effect were all higher than the additive-free beverage. A, B and C were excellent, especially the added beverage A was the highest.
실시예 7: 눈꽃동충하초 고분자 다당체 함유 음료의 기능성 평가Example 7: Functional Evaluation of Snowflake Cordyceps Polymer Polysaccharide-Containing Beverages
실시예 4에서 제조한 동충하초 액체배양으로부터 생산된 고분자다당체를 첨가하여 제조한 기능성 음료의 주요 기능성을 상기 실시예 5와 동일한 방법으로 평가하였으며 결과를 표 3에 나타냈다. 무첨가음료는 동충하초 액체배양액에 함유된 동결건조 고분자 다당체를 첨가하지 않은 대조구이며, 첨가음료 A는 동충하초 액체배양액에 함유된 동결건조 고분자 다당체 6g/L, 첨가음료 B는 4g/L, 첨가음료 C는 2g/L를 각각 첨가하여 제조한 기능성 음료이다. 표 3에 나타낸 바와 같이 면역활성은 무첨가음료에 비해 첨가음료 A, B, C가 모두 높았으며 특히 첨가음료 A가 높았고 혈중콜레스테롤 저하효과, 혈당저하효과 및 운동력 증강효과에서도 모두 무첨가음료에 비해 첨가음료 A, B, C가 우수하였으며 특히 첨가음료 A가 가장 높았다.The main functionality of the functional beverage prepared by adding the polymer polysaccharide produced from the Cordyceps sinensis liquid culture prepared in Example 4 was evaluated in the same manner as in Example 5, and the results are shown in Table 3. The additive-free beverage is a control group without addition of the lyophilized polymer polysaccharide contained in the Cordyceps liquor culture medium.Additional drink A is 6g / L of the lyophilized polymer polysaccharide contained in the Cordyceps liquor liquid culture medium, 4g / L of the beverage B added, and beverage C is A functional beverage prepared by adding 2 g / L, respectively. As shown in Table 3, Immune activity was higher in all of the added beverages A, B, and C than in the non-added beverages, and in particular, the added beverage A was high, and in addition, in the blood cholesterol lowering effect, hypoglycemic effect, and exercise enhancing effect, all the additive beverages were added. A, B and C were excellent, especially the added beverage A was the highest.
상기 실시예를 통하여 설명한 바와 같이 눈꽃동충하초 균사체와 고분자다당체를 모두 또는 그 중 어느하나를 포함하는 액체배양물과 식품첨가물을 열정제수 및 막여과수에 적정량 배합하여 제조한 본 발명 눈꽃동충하초 기능성 음료는 인체면역증강, 혈중콜레스테롤저하, 혈당저하, 운동력증강과 같은 인체 생리활성을 증강시키는 뛰어난 효과가 있으므로 건강식품 산업상 매우 유용한 발명인 것이다.Snowflake Cordyceps functional beverage of the present invention prepared by mixing a suitable amount of liquid culture and food additives containing all or any one of the Snow Cordyceps fungus mycelium and the polymer polysaccharide as described above in the present invention It is a very useful invention in the health food industry because it has an excellent effect of enhancing the body's physiological activities such as immune enhancement, blood cholesterol lowering, blood sugar lowering, exercise strengthening.
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| KR1019990027106A KR100347267B1 (en) | 1999-07-06 | 1999-07-06 | Functional beverage containing submerged culture broth of Paecilomyces sp. and process for preparation thereof |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20030013218A (en) * | 2001-08-06 | 2003-02-14 | 김홍남 | Cordyceps sinensis cultivation technology using mycelial activation method and fruiting body extract healthy drink using the raw material |
| KR20030013219A (en) * | 2001-08-06 | 2003-02-14 | 김홍남 | Development of Cordyceps and Cultivation Technology and Manufacturing Technology Using Microbial Fermentation Medium and Fruiting Body Extracts |
| WO2003040348A1 (en) * | 2001-10-19 | 2003-05-15 | Mushtech, Inc. | Schimizuomyces sp. sjm, the method of culture thereof and the composition for plant growth promoting including culture solution thereof |
| KR20050014237A (en) * | 2003-07-30 | 2005-02-07 | 학교법인 영광학원 | Functional Soju containing submerged culture broth of mushrooms and process for preparation thereof |
| KR100505217B1 (en) * | 2002-03-30 | 2005-07-29 | 문창현 | Compositions comprising Isaria sinclairii as an effective component |
| KR100559998B1 (en) * | 2005-04-30 | 2006-03-15 | 문창현 | Food compositions comprising Isaria sinclairii as an effective component |
| KR100695507B1 (en) * | 2001-01-15 | 2007-03-15 | 가부시키가이샤 시마노 | Cooler box |
| KR100754821B1 (en) * | 2006-03-13 | 2007-09-03 | 학교법인 영광학원 | Method of mass production for mycelia and extracellular polysaccharides of the same cordyceps sphecocephala |
| WO2008080937A1 (en) * | 2006-12-28 | 2008-07-10 | Basf Se | 2-substituted pyrimidines i in therapy |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100391181B1 (en) * | 2000-12-29 | 2003-07-12 | 신화제약 (주) | The manufacturing method of exopolysaccharide by liquid culturing of Paecilomyces tenuipes |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR19980082143A (en) * | 1998-08-28 | 1998-11-25 | 조성태 | Cordyceps mycelium and fruiting body forming method and the difference using the cordyceps formed by the method |
-
1999
- 1999-07-06 KR KR1019990027106A patent/KR100347267B1/en not_active IP Right Cessation
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100695507B1 (en) * | 2001-01-15 | 2007-03-15 | 가부시키가이샤 시마노 | Cooler box |
| KR20030013218A (en) * | 2001-08-06 | 2003-02-14 | 김홍남 | Cordyceps sinensis cultivation technology using mycelial activation method and fruiting body extract healthy drink using the raw material |
| KR20030013219A (en) * | 2001-08-06 | 2003-02-14 | 김홍남 | Development of Cordyceps and Cultivation Technology and Manufacturing Technology Using Microbial Fermentation Medium and Fruiting Body Extracts |
| WO2003040348A1 (en) * | 2001-10-19 | 2003-05-15 | Mushtech, Inc. | Schimizuomyces sp. sjm, the method of culture thereof and the composition for plant growth promoting including culture solution thereof |
| KR100505217B1 (en) * | 2002-03-30 | 2005-07-29 | 문창현 | Compositions comprising Isaria sinclairii as an effective component |
| KR20050014237A (en) * | 2003-07-30 | 2005-02-07 | 학교법인 영광학원 | Functional Soju containing submerged culture broth of mushrooms and process for preparation thereof |
| KR100559998B1 (en) * | 2005-04-30 | 2006-03-15 | 문창현 | Food compositions comprising Isaria sinclairii as an effective component |
| KR100754821B1 (en) * | 2006-03-13 | 2007-09-03 | 학교법인 영광학원 | Method of mass production for mycelia and extracellular polysaccharides of the same cordyceps sphecocephala |
| WO2008080937A1 (en) * | 2006-12-28 | 2008-07-10 | Basf Se | 2-substituted pyrimidines i in therapy |
Also Published As
| Publication number | Publication date |
|---|---|
| KR100347267B1 (en) | 2002-11-16 |
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