KR102639762B1 - Plant nutrient composition using clover extract - Google Patents
Plant nutrient composition using clover extract Download PDFInfo
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- KR102639762B1 KR102639762B1 KR1020230086259A KR20230086259A KR102639762B1 KR 102639762 B1 KR102639762 B1 KR 102639762B1 KR 1020230086259 A KR1020230086259 A KR 1020230086259A KR 20230086259 A KR20230086259 A KR 20230086259A KR 102639762 B1 KR102639762 B1 KR 102639762B1
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- clover extract
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/20—Fabaceae or Leguminosae [Pea or Legume family], e.g. pea, lentil, soybean, clover, acacia, honey locust, derris or millettia
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
- A01N37/46—N-acyl derivatives
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N55/00—Biocides, pest repellants or attractants, or plant growth regulators, containing organic compounds containing elements other than carbon, hydrogen, halogen, oxygen, nitrogen and sulfur
- A01N55/02—Biocides, pest repellants or attractants, or plant growth regulators, containing organic compounds containing elements other than carbon, hydrogen, halogen, oxygen, nitrogen and sulfur containing metal atoms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P21/00—Plant growth regulators
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N2300/00—Combinations or mixtures of active ingredients covered by classes A01N27/00 - A01N65/48 with other active or formulation relevant ingredients, e.g. specific carrier materials or surfactants, covered by classes A01N25/00 - A01N65/48
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Environmental Sciences (AREA)
- Plant Pathology (AREA)
- Wood Science & Technology (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Dentistry (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Biotechnology (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Botany (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
본 발명은 토끼풀 추출물을 이용한 식물영양제 조성물에 관한 것으로, 더욱 상세하게는 토끼풀 추출물이 40 내지 60 중량% 함유되어 이루어진다.
상기와 같이 토끼풀 추출물이 함유된 식물영양제는 다량의 아미노산과 질소 고정 박테리아가 함유된 토끼풀 추출물로 인해 대기 중에 있는 질소 분자를 식물이 이용할 수 있는 질화 화합물로 바꿔주기 때문에, 우수한 식물성장 효과를 나타낸다.The present invention relates to a plant nutritional composition using a clover extract, and more specifically, it contains 40 to 60% by weight of a clover extract.
As described above, plant nutrients containing clover extract show excellent plant growth effects because the clover extract, which contains a large amount of amino acids and nitrogen-fixing bacteria, changes nitrogen molecules in the air into nitrifying compounds that plants can use.
Description
본 발명은 토끼풀 추출물을 이용한 식물영양제 조성물에 관한 것으로, 더욱 상세하게는 다량의 아미노산과 질소 고정 박테리아가 함유된 토끼풀 추출물로 인해 대기 중에 있는 질소 분자를 식물이 이용할 수 있는 질화 화합물로 바꿔주기 때문에, 우수한 식물성장 효과를 나타내는 토끼풀 추출물을 이용한 식물영양제 조성물에 관한 것이다.The present invention relates to a plant nutritional composition using a clover extract. More specifically, the clover extract, which contains a large amount of amino acids and nitrogen-fixing bacteria, changes nitrogen molecules in the air into nitrifying compounds that plants can use, It relates to a plant nutritional composition using clover extract that exhibits excellent plant growth effects.
천연 자원으로부터 식물생장에 도움을 주는 식물영양제의 개발은 전문가들도 충분한 잠재력을 인정하지만, 유효추출물을 경제적이고 저렴하게 추출할 수 있는 방법도출이 어렵다. 그럼에도 많은 연구가 지속적으로 이루어지고 있는 실정이다.Experts recognize that the development of plant nutrients that help plant growth from natural resources has ample potential, but it is difficult to derive a method to extract effective extracts economically and inexpensively. Nevertheless, many studies are continuously being conducted.
우선 식물계 바이오매스 자원은 잠재력이 무한한 유기자원임과 동시에 천연 무공해 자원이며, 자연환경의 범주에서 자연적인 순환과 반복에 의해 생산되는 자원이다.First of all, plant-based biomass resources are organic resources with infinite potential and at the same time are natural, pollution-free resources, and are produced through natural circulation and repetition within the natural environment.
그러나, 식물계 바이오매스 자원은 경제적인 측면에서 운반 및 고부가가치가 낮기에 대부분 자연 물질 순환에 의해 분해된다. 그러나 식물계 바이오매스 자원은 거대한 탄소 집합체로서 식물에 필요한 모든 영양원을 보유하고 있기에 식물영양제로서의 잠재력을 가지고 있다.However, because plant-based biomass resources have low transport and added value in economic terms, they are mostly decomposed through natural material circulation. However, plant-based biomass resources are huge carbon aggregates and contain all the nutrients necessary for plants, so they have the potential as plant nutrients.
한편, 토끼풀은 잔디밭이나 밭두렁에 토끼 꼬리처럼 생긴 꽃이 피는 식물로, 하트 모양의 작은 잎이 세 장씩 모여 난다. 토끼풀은 '클로버'라는 영어 이름으로 많이 알려져 있는데, 일반적으로 세 장의 작은잎을 가진 겹잎을 내지만, 매우 낮은 확률로 유전적 돌연변이가 발생해 네 장 이상의 잎을 내기도 합니다. 네 장의 작은 잎을 가진 토끼풀 잎을 발견한 사람에게는 행운이 찾아온다는 속설은 유명하다.Meanwhile, clover is a plant that blooms on lawns or ridges with flowers shaped like a rabbit's tail, and has three small heart-shaped leaves. Shamrock is widely known by its English name 'clover'. It generally produces compound leaves with three small leaves, but there is a very low probability that a genetic mutation may occur, causing it to produce four or more leaves. The popular belief is that good luck will come to anyone who finds a clover leaf with four small leaves.
토끼풀이 자라는 토양은 질소 고정 박테리아가 만들어내는 질소 화합물로 인해 비옥해지는 특성이 있기 때문에 밭작물을 기를 때 함께 기르거나 농사를 쉬는 시기에 밭에 길러 토양을 비옥하게 하는 용도로 재배되고 있다.Because the soil in which clover grows is fertile due to the nitrogen compounds produced by nitrogen-fixing bacteria, it is grown together with field crops or grown in fields during the off season to fertilize the soil.
그러나, 종래에는 토끼풀 추출물을 화장품 등에 활용했을 뿐, 상기의 효과를 나타내는 토끼풀을 이용하여 식물영양제로 활용하는 기술은 검색되지 않았다.However, in the past, clover extract was only used in cosmetics, etc., and no technology was found for using clover extract as a plant nutrient that exhibits the above-mentioned effects.
본 발명의 목적은 다량의 아미노산과 질소 고정 박테리아가 함유된 토끼풀 추출물로 인해 대기 중에 있는 질소 분자를 식물이 이용할 수 있는 질화 화합물로 바꿔주기 때문에, 우수한 식물성장 효과를 나타내는 토끼풀 추출물을 이용한 식물영양제 조성물을 제공하는 것이다.The object of the present invention is to provide a plant nutritional composition using a clover extract that exhibits excellent plant growth effects because the clover extract, which contains a large amount of amino acids and nitrogen-fixing bacteria, changes nitrogen molecules in the air into nitrifying compounds that plants can use. is to provide.
본 발명의 다른 목적은 유기 킬레이트 셀레늄과 아미노산발효부산물액으로 이루어진 그룹에서 선택된 하나 이상이 더 함유되어 식물성장 효과가 더욱 향상될 뿐만 아니라, 친환경적인 식물영양제 조성물을 제공하는 것이다.Another object of the present invention is to provide an environmentally friendly plant nutrient composition that not only further improves the plant growth effect by further containing one or more selected from the group consisting of organic chelated selenium and amino acid fermentation by-product liquid.
본 발명의 목적은 토끼풀 추출물이 40 내지 60 중량% 함유되는 것을 특징으로 하는 토끼풀 추출물을 이용한 식물영양제 조성물을 제공함에 의해 달성된다.The object of the present invention is achieved by providing a plant nutritional composition using a clover extract, characterized in that it contains 40 to 60% by weight of the clover extract.
본 발명의 바람직한 특징에 따르면, 상기 토끼풀 추출물은 토끼풀 건조분말 100 중량부에 질량농도가 65 내지 75%인 에탄올 800 내지 1200 중량부를 혼합하고 75 내지 85℃의 온도에서 10 내지 15시간 동안 환류추출하여 제조되는 것으로 한다.According to a preferred feature of the present invention, the clover extract is obtained by mixing 800 to 1,200 parts by weight of ethanol having a mass concentration of 65 to 75% with 100 parts by weight of dried clover powder and extracting under reflux for 10 to 15 hours at a temperature of 75 to 85 ° C. It is assumed to be manufactured.
본 발명의 더 바람직한 특징에 따르면, 상기 토끼풀 추출물을 이용한 식물영양제 조성물에는 상기 토끼풀 추출물 100 중량부 대비 유기 킬레이트 셀레늄 5 내지 10 중량부가 더 함유되는 것으로 한다.According to a more preferred feature of the present invention, the plant nutritional composition using the clover extract contains 5 to 10 parts by weight of organic chelated selenium based on 100 parts by weight of the clover extract.
본 발명의 더욱 바람직한 특징에 따르면, 상기 유기 킬레이트 셀레늄은 질량농도가 20 내지 50%인 EDTA 수용액 100 중량부에 수산화칼륨 2 내지 15 중량부와 산화셀레늄 2 내지 10 중량부를 차례로 투입하고 반응시켜 제조되는 것으로 한다.According to a more preferred feature of the present invention, the organic chelated selenium is prepared by sequentially adding 2 to 15 parts by weight of potassium hydroxide and 2 to 10 parts by weight of selenium oxide to 100 parts by weight of an EDTA aqueous solution having a mass concentration of 20 to 50% and reacting. Let's do it.
본 발명의 더욱 더 바람직한 특징에 따르면, 상기 토끼풀 추출물을 이용한 식물영양제 조성물에는 상기 토끼풀 추출물 100 중량부 대비 아미노산발효부산물액 10 내지 20 중량부가 더 함유되는 것으로 한다.According to an even more preferred feature of the present invention, the plant nutritional composition using the clover extract contains 10 to 20 parts by weight of amino acid fermentation by-product liquid compared to 100 parts by weight of the clover extract.
본 발명의 더욱 더 바람직한 특징에 따르면, 상기 아미노산발효부산물액은 아미노산으로서 라이신(lysine)의 생산과정 중에 얻어지는 라이신발효부산물액 또는 글루타민산(glutamic acid)의 생산과정 중에 얻어지는 글루타민산발효부산물액으로 이루어지는 것으로 한다.According to an even more preferred feature of the present invention, the amino acid fermentation by-product liquid is composed of a lysine fermentation by-product liquid obtained during the production process of lysine as an amino acid or a glutamic acid fermentation by-product liquid obtained during the production process of glutamic acid. .
본 발명에 따른 토끼풀 추출물을 이용한 식물영양제 조성물은 다량의 아미노산과 질소 고정 박테리아가 함유된 토끼풀 추출물로 인해 대기 중에 있는 질소 분자를 식물이 이용할 수 있는 질화 화합물로 바꿔주기 때문에, 우수한 식물성장 효과를 나타내는 식물영양제 조성물을 제공하는 탁월한 효과를 나타낸다.The plant nutritional composition using the clover extract according to the present invention exhibits excellent plant growth effects because the clover extract containing a large amount of amino acids and nitrogen-fixing bacteria changes nitrogen molecules in the air into nitrifying compounds that plants can use. It shows excellent effectiveness in providing plant nutritional compositions.
또한, 유기 킬레이트 셀레늄과 아미노산발효부산물액으로 이루어진 그룹에서 선택된 하나 이상이 더 함유되어 식물성장 효과가 더욱 향상될 뿐만 아니라, 친환경적인 식물영양제 조성물을 제공하는 탁월한 효과를 나타낸다.In addition, the addition of one or more selected from the group consisting of organic chelated selenium and amino acid fermentation by-product liquid not only further improves the plant growth effect, but also provides an excellent effect of providing an eco-friendly plant nutrient composition.
이하에는, 본 발명의 바람직한 실시예와 각 성분의 물성을 상세하게 설명하되, 이는 본 발명이 속하는 기술분야에서 통상의 지식을 가진 자가 발명을 용이하게 실시할 수 있을 정도로 상세하게 설명하기 위한 것이지, 이로 인해 본 발명의 기술적인 사상 및 범주가 한정되는 것을 의미하지는 않는다.Below, preferred embodiments of the present invention and the physical properties of each component are described in detail, but are intended to be described in detail so that a person skilled in the art can easily carry out the invention. This does not mean that the technical idea and scope of the present invention are limited.
본 발명에 따른 토끼풀 추출물을 이용한 식물영양제 조성물은 토끼풀 추출물이 함유되며, 토끼풀 추출물이 40 내지 60 중량% 함유되는 것이 바람직하며, 토끼풀 추출물을 제외한 나머지 잔부는 정제수로 이루어지는 것이 바람직하다.The plant nutritional composition using the clover extract according to the present invention contains the clover extract, preferably 40 to 60% by weight of the clover extract, and the remainder excluding the clover extract is preferably made of purified water.
상기 토끼풀 추출물은 토끼풀 건조분말 100 중량부에 질량농도가 65 내지 75%인 에탄올 800 내지 1200 중량부를 혼합하고 75 내지 85℃의 온도에서 10 내지 15시간 동안 환류추출하여 제조되는 것이 바람직하다.The clover extract is preferably prepared by mixing 800 to 1,200 parts by weight of ethanol with a mass concentration of 65 to 75% with 100 parts by weight of dried clover powder and performing reflux extraction at a temperature of 75 to 85 ° C. for 10 to 15 hours.
토끼풀은 콩이나 아까시나무와 함께 콩과(科)에 속하는 식물로, 다른 콩과 식물들과 마찬가지로 뿌리에 '질소 고정 박테리아'가 살고 있는데, 토끼풀 뿌리에서 함께 사는 질소 고정 박테리아는 대기 중에 있는 질소 분자를 식물이 이용할 수 있는 형태의 질소 화합물로 바꿔 토끼풀이 이용할 수 있게 도와준다. 그 대신 이 박테리아는 토끼풀 뿌리에서 토끼풀이 합성한 아미노산(단백질의 재료가 되는 물질)을 받아 사용는 '공생 관계'다. 토끼풀이 가축 사료나 건초로 쓰이는 건, 단백질 함량이 높기 때문인데, 질소 고정 박테리아가 제공하는 풍부한 질소가 토끼풀 안에서 단백질로 합성되기 때문이다.Clover is a plant that belongs to the legume family along with beans and locust. Like other legumes, it has 'nitrogen-fixing bacteria' living in its roots. The nitrogen-fixing bacteria that live together in the roots of the clover are molecules of nitrogen in the air. It helps clover to use nitrogen compounds by converting them into nitrogen compounds that plants can use. Instead, this bacterium has a 'symbiotic relationship' in which it receives and uses the amino acids (substances that make up proteins) synthesized by the clover from the roots of the clover. The reason why clover is used as livestock feed or hay is because it has a high protein content, and the abundant nitrogen provided by nitrogen-fixing bacteria is synthesized into protein within the clover.
따라서, 상기의 다량의 아미노산과 질소 고정 박테리아가 함유된 토끼풀 추출물로 인해 대기 중에 있는 질소 분자를 식물이 이용할 수 있는 질화 화합물로 바꿔주기 때문에, 우수한 식물성장 효과를 나타내는 식물영양제 조성물을 제공할 수 있게 된다.Therefore, the clover extract containing a large amount of amino acids and nitrogen-fixing bacteria changes nitrogen molecules in the air into nitrifying compounds that plants can use, making it possible to provide a plant nutrient composition that exhibits excellent plant growth effects. do.
상기 토끼풀 추출물의 함량이 40 중량% 미만이면 상기의 효과가 미미하며, 상기 토끼풀 추출물의 함량이 60 중량%를 초과하게 되면 상기의 효과는 크게 향상되지 않으면서 제조비용을 지나치게 증가시키기 때문에 바람직하지 못하다.If the content of the clover extract is less than 40% by weight, the effect is minimal, and if the content of the clover extract exceeds 60% by weight, the effect is not significantly improved and the manufacturing cost is excessively increased, which is not desirable. .
또한, 본 발명에 따른 토끼풀 추출물을 이용한 식물영양제 조성물에는 상기 토끼풀 추출물 100 중량부 대비 유기 킬레이트 셀레늄 5 내지 10 중량부가 더 함유될 수도 있는데, 상기 유기 킬레이트 셀레늄은 질량농도가 20 내지 50%인 EDTA 수용액 100 중량부에 수산화칼륨 2 내지 15 중량부와 산화셀레늄 2 내지 10 중량부를 차례로 투입하고 반응시켜 제조되는 것이 바람직하다.In addition, the phytonutrient composition using the clover extract according to the present invention may further contain 5 to 10 parts by weight of organic chelated selenium compared to 100 parts by weight of the clover extract, and the organic chelated selenium is an EDTA aqueous solution with a mass concentration of 20 to 50%. It is preferably prepared by sequentially adding 2 to 15 parts by weight of potassium hydroxide and 2 to 10 parts by weight of selenium oxide to 100 parts by weight and reacting.
상기의 과정을 통해 제조되는 유기 킬레이트 셀레늄은 일반 셀레늄에 비해 식물에 흡수되는 효과가 30% 이상 향상되어 적은양으로도 우수한 식물성장 효과를 나타내는 식물영양제가 제공될 수 있도록 하는 역할을 한다.Organic chelated selenium manufactured through the above process has an improved absorption effect by plants by more than 30% compared to regular selenium, thereby providing a plant nutrient that exhibits excellent plant growth effects even in small amounts.
상기 유기 킬레이트 셀레늄의 함량이 5 중량부 미만이면 상기의 효과가 미미하며, 상기 유기 킬레이트 셀레늄의 함량이 10 중량부를 초과하게 되면 상기의 효과는 크게 향상되지 않으면서 상대적으로 토끼풀 추출물의 함량이 지나치게 줄어들고 식물영양제 조성물의 제조비용을 지나치게 증가시키기 때문에 바람직하지 못하다.If the content of the organic chelated selenium is less than 5 parts by weight, the effect is minimal, and if the content of the organic chelated selenium exceeds 10 parts by weight, the effect is not significantly improved and the content of the clover extract is relatively reduced excessively. This is undesirable because it excessively increases the manufacturing cost of the plant nutritional composition.
또한, 본 발명에 따른 토끼풀 추출물을 이용한 식물영양제 조성물에는 상기 토끼풀 추출물 100 중량부 대비 아미노산발효부산물액 10 내지 20 중량부가 더 함유될 수도 있는데, 상기 아미노산발효부산물액은 아미노산으로서 라이신(lysine)의 생산과정 중에 얻어지는 라이신발효부산물액 또는 글루타민산(glutamic acid)의 생산과정 중에 얻어지는 글루타민산발효부산물액으로 이루어지는 것이 바람직하다.In addition, the plant nutritional composition using the clover extract according to the present invention may contain an additional 10 to 20 parts by weight of amino acid fermentation by-product liquid compared to 100 parts by weight of the clover extract, and the amino acid fermentation by-product liquid is used for the production of lysine as an amino acid. It is preferably composed of a lysine fermentation by-product liquid obtained during the process or a glutamic acid fermentation by-product liquid obtained during the production process of glutamic acid.
상기의 아미노산발효부산물액은 식물성장에 필요한 질소, 인 및 칼륨 등이 모두 함유되어 있을 뿐만 아니라, 각종 비타민이 다량 함유되어 식물의 야간성장을 촉진하며, 착과 및 비대의 효과를 나타내어 과실의 착색과 당도를 증진시킬 뿐만 아니라, 저장성과 비타민의 함량을 증가시키며 이상기후에서도 건전한 성장이 이루어질 수 있도록 하는 역할을 한다.[농업진흥청 국립농업과학원 유기농정보센터 홈페이지 참조]The amino acid fermentation by-product liquid not only contains all the nitrogen, phosphorus, and potassium necessary for plant growth, but also contains a large amount of various vitamins, promoting night-time growth of plants, and has the effect of fruit set and enlargement, causing fruit coloring and growth. It not only improves sugar content, but also increases storability and vitamin content, and plays a role in ensuring healthy growth even in abnormal climates. [Refer to the Organic Information Center website of the National Institute of Agricultural Science, Agricultural Promotion Agency]
일 예로 CMS(condensed molasses solubles)의 탈염 및 농축을 통해 얻어지는 라이신발효부산물액의 조성비를 분석하여 아래 표 1에 나타내었다.As an example, the composition ratio of the lysine fermentation by-product liquid obtained through desalting and concentration of CMS (condensed molasses solubles) was analyzed and is shown in Table 1 below.
<표 1><Table 1>
이때, 상기 조단백(crude protein)은 아스팔틱산(aspartic Acid), 트레오닌(threonine), 세린(serine), 글루타민산(glutamic acid), 프로틴(protein), 글리신(glycine), 알라닌(alanine), 발린(valine), 아이소루신(isoleucine), 류신(leucine), 타이로닌(thyronine), 페닐알라닌(phenylalanine), 히스티딘(histidine), 라이신(lysine), 알지닌(arginine) 중 적어도 하나를 포함하고, 상기 조섬유(crude fiber)는 셀루로오즈(cellulose), 리그닌(lignin) 중 적어도 하나를 포함하며, 상기 조회분(crude ash)은 질소(N), 칼슘(Ca), 인(P), 나트륨(Na), 칼륨(K), 마그네슘(Mg), 염소(Cl), 황(S) 중 적어도 하나를 포함하고, 상기 기타 유기물은 비타민 B1, B2, B6, B12, 니코틴산(nicotinic acid), 판토텐산(pantothenic acid), 비오틴(biotin) 및 콜린(choline) 중 적어도 하나를 포함한다.At this time, the crude protein contains aspartic acid, threonine, serine, glutamic acid, protein, glycine, alanine, and valine ( Contains at least one of valine, isoleucine, leucine, thyronine, phenylalanine, histidine, lysine, and arginine, and the crude fiber ( Crude fiber contains at least one of cellulose and lignin, and the crude ash contains nitrogen (N), calcium (Ca), phosphorus (P), sodium (Na), It contains at least one of potassium (K), magnesium (Mg), chlorine (Cl), and sulfur (S), and the other organic substances include vitamins B 1 , B 2 , B 6 , B 12 , nicotinic acid, and pantothenic acid. Contains at least one of (pantothenic acid), biotin, and choline.
상기 아미노산발효부산물액의 함량이 10 중량부 미만이면 상기의 효과가 미미하며, 상기 아미노산발효부산물액의 함량이 20 중량부를 초과하게 되면 상기의 효과는 크게 향상되지 않으면서 상대적으로 토끼풀 추출물의 함량이 지나치게 줄어들기 때문에 바람직하지 못하다.If the content of the amino acid fermentation by-product liquid is less than 10 parts by weight, the above effect is minimal, and if the content of the amino acid fermentation by-product liquid exceeds 20 parts by weight, the above effect is not significantly improved and the content of the clover extract is relatively high. This is undesirable because it decreases excessively.
이하에서는, 본 발명에 따른 토끼풀 추출물을 이용한 식물영양제 조성물의 제조방법 및 그 제조방법으로 제조된 식물영양제 조성물의 물성을 실시예를 들어 설명하기로 한다.Hereinafter, the method for producing a plant nutritional composition using the clover extract according to the present invention and the physical properties of the plant nutritional composition prepared by the manufacturing method will be described using examples.
<제조예 1> 토끼풀 추출물의 제조<Preparation Example 1> Preparation of clover extract
토끼풀 건조분말 100 중량부에 질량농도가 70%인 에탄올 1000 중량부를 혼합하고 80℃의 온도에서 12시간 동안 환류추출하여 토끼풀 추출물을 제조하였다.A clover extract was prepared by mixing 1000 parts by weight of ethanol with a mass concentration of 70% with 100 parts by weight of dried clover powder and performing reflux extraction for 12 hours at a temperature of 80°C.
<제조예 2> 유기 킬레이트 셀레늄의 제조<Preparation Example 2> Preparation of organic chelate selenium
정제수 70g에 EDTA(ethylendiaminetetraacetic acid) 20g을 투입 후 교반하여 제조된 EDTA 수용액에 수산화칼륨 15g을 서서히 투입하면서 2시간 이상 교반하여 1차 반응 진행하였으며, 1차 반응이 완료(맑은 용액의 상태로 약간의 발열 반응이 발생)되며, 반응액에 SeO2(Selenium oxide) 75g을 서서히 투입하고 12시간 이상 반응시켜 유기 킬레이트 셀레늄을 제조하였다.The first reaction was carried out by slowly adding 15 g of potassium hydroxide to the EDTA aqueous solution prepared by adding 20 g of EDTA (ethylendiaminetetraacetic acid) to 70 g of purified water and stirring for more than 2 hours. The first reaction was completed (a clear solution with a slight odor). An exothermic reaction occurred), and 75 g of SeO 2 (Selenium oxide) was slowly added to the reaction solution and reacted for more than 12 hours to prepare organic chelate selenium.
<실시예 1><Example 1>
상기 제조예 1을 통해 제조된 토끼풀 추출물과 정제수를 1:1의 중량부로 혼합하여 토끼풀 추출물을 이용한 식물영양제 조성물을 제조하였다.A plant nutritional composition using the clover extract prepared in Preparation Example 1 was prepared by mixing the clover extract prepared in Preparation Example 1 and purified water in a ratio of 1:1 by weight.
<실시예 2><Example 2>
상기 실시예 1과 동일하게 진행하되, 상기 실시예 1을 통해 제조된 토끼풀 추출물을 이용한 식물영양제 조성물에 함유된 토끼풀 추출물 100 중량부 대비 상기 제조예 2를 통해 제조된 유기 킬레이트 셀레늄 8 중량부를 더 혼합하여 토끼풀 추출물을 이용한 식물영양제 조성물을 제조하였다.Proceed in the same manner as in Example 1, except that 8 parts by weight of organic chelate selenium prepared in Preparation Example 2 was further mixed with 100 parts by weight of the clover extract contained in the plant nutritional composition using the clover extract prepared in Example 1. A plant nutritional composition using clover extract was prepared.
<실시예 3><Example 3>
상기 실시예 1과 동일하게 진행하되, 상기 실시예 1을 통해 제조된 토끼풀 추출물을 이용한 식물영양제 조성물에 함유된 토끼풀 추출물 100 중량부 대비 아미노산으로서 라이신(lysine)의 생산과정 중에 얻어지는 라이신발효부산물액("KG AGRO" 사(社)의 상품명 "CMS 액") 15 중량부를 더 혼합하여 토끼풀 추출물을 이용한 식물영양제 조성물을 제조하였다.Proceed in the same manner as in Example 1, except that lysine fermentation by-product liquid (lysine) obtained during the production of lysine as an amino acid relative to 100 parts by weight of the clover extract contained in the plant nutritional composition using the clover extract prepared in Example 1 ( A plant nutritional composition using clover extract was prepared by further mixing 15 parts by weight of "KG AGRO" (trade name "CMS liquid").
<실시예 4><Example 4>
상기 실시예 1과 동일하게 진행하되, 상기 실시예 1을 통해 제조된 토끼풀 추출물을 이용한 식물영양제 조성물에 함유된 토끼풀 추출물 100 중량부 대비 상기 제조예 2를 통해 제조된 유기 킬레이트 셀레늄 8 중량부 및 아미노산으로서 라이신(lysine)의 생산과정 중에 얻어지는 라이신발효부산물액("KG AGRO" 사(社)의 상품명 "CMS 액") 15 중량부를 더 혼합하여 토끼풀 추출물을 이용한 식물영양제 조성물을 제조하였다.Proceed in the same manner as in Example 1, except that 8 parts by weight of organic chelated selenium prepared in Preparation Example 2 and amino acids are added to 100 parts by weight of the clover extract contained in the plant nutritional composition using the clover extract prepared in Example 1. A plant nutritional composition using the clover extract was prepared by further mixing 15 parts by weight of the lysine fermentation by-product liquid obtained during the production of lysine (trade name "CMS liquid" of "KG AGRO").
<비교예 1><Comparative Example 1>
포도 식물영양제 조성물{(주)대유 사, 상품번호:8268210885}.Grape plant nutritional composition {Dayyu Co., Ltd., product number: 8268210885}.
상기 실시예 1 내지 4 및 비교예 1의 식물영양제 조성물의 식물성장 효과(칼슘 함량)를 측정하여 아래 표 2에 나타내었다.The plant growth effects (calcium content) of the plant nutritional compositions of Examples 1 to 4 and Comparative Example 1 were measured and shown in Table 2 below.
{단, 식물성장 효과는 캠벨얼리 종의 포도를 재배하는 과정으로 측정하였으며, 실시예 1 내지 4 및 비교예 1의 식물영양제 조성물을 물에 500배 희석한 후, 개화 7~21일 후에 각각 엽면시비하였다. 이때 시비량은 12ℓ/300평으로 하였다.}{However, the plant growth effect was measured in the process of cultivating Campbell's Early grapes, and the plant nutrient compositions of Examples 1 to 4 and Comparative Example 1 were diluted 500 times in water and then foliar fertilized 7 to 21 days after flowering. did. At this time, the fertilization amount was 12 liters/300 pyeong.}
<표 2><Table 2>
상기 표 1에 나타낸 것처럼, 본 발명의 실시예 1 내지 4를 통해 제조된 식물영양제 조성물을 엽면시비하여 제조된 포도는 비교예 1의 식물영양제 조성물을 엽면시비하여 재배한 포도에 비해 과피에 칼슘함량이 월등하게 향상되는 것을 알 수 있다.As shown in Table 1, grapes produced by foliar application of the plant nutrient composition prepared in Examples 1 to 4 of the present invention have a higher calcium content in the skin than grapes grown by foliar application of the plant nutrient composition of Comparative Example 1. You can see that this has improved significantly.
또한, 상기 실시예 1 내지 4 및 비교예 1의 식물영양제 조성물의 식물성장 효과(당도, 경도)를 측정하여 아래 표 3에 나타내었다.In addition, the plant growth effects (sugar content, hardness) of the plant nutritional compositions of Examples 1 to 4 and Comparative Example 1 were measured and shown in Table 3 below.
{이때, 당도는 굴절당도계(일본 ATAGO사, 모델명:PAL-2를 이용하여 측정하였으며, 경도는 과일경도측정기(Feruit Hardness Tester, cat No 9300, Japan)를 이용하여 측정하였다.}{At this time, the sugar content was measured using a refractometer (ATAGO, Japan, model name: PAL-2), and the hardness was measured using a fruit hardness tester (cat No 9300, Japan).}
<표 3> <Table 3>
상기 표 3에 나타낸 것처럼, 본 발명의 실시예 1 내지 4를 통해 제조된 식물영양제 조성물을 엽면시비하여 재배한 포되는 비교예 1의 식물영양제 조성물을 엽면시비하여 제조된 포도에 비해 당도 및 경도가 매우 향상되는 것을 알 수 있다.As shown in Table 3, grapes grown by foliar application of the plant nutrient composition prepared in Examples 1 to 4 of the present invention had lower sugar content and hardness than grapes prepared by foliar application of the plant nutrient composition of Comparative Example 1. You can see that it is greatly improved.
또한, 상기 실시예 1 내지 4 및 비교예 1의 식물영양제 조성물의 식물성장 효과(포도의 크기)를 측정하여 아래 표 4에 나타내었다.In addition, the plant growth effect (grape size) of the plant nutritional compositions of Examples 1 to 4 and Comparative Example 1 was measured and shown in Table 4 below.
{이때, 포도의 크기는 과중, 과장, 과폭, 과립수 및 과립중을 측정하는 방법을 이용하였다.}{At this time, the size of the grapes was measured by measuring fruit weight, exaggeration, fruit width, number of granules, and weight of granules.}
<표 4><Table 4>
상기 표 4에 나타낸 것처럼, 본 발명의 실시예 1 내지 4를 통해 제조된 식물영양제 조성물을 엽면시비하여 재배된 포도는 비교예 1의 식물영양제 조성물을 엽면시비하여 제조된 포도에 비해 포도의 과중, 과장, 과폭, 과립수 및 과립중이 모두 향상되는 것을 알 수 있다.As shown in Table 4, grapes grown by foliar application of the plant nutrient composition prepared in Examples 1 to 4 of the present invention have a higher weight compared to grapes prepared by foliar application of the plant nutrient composition of Comparative Example 1. It can be seen that exaggeration, exaggeration, granule number, and granule weight are all improved.
따라서, 본 발명에 따른 토끼풀 추출물을 이용한 식물영양제 조성물은 다량의 아미노산과 질소 고정 박테리아가 함유된 토끼풀 추출물로 인해 대기 중에 있는 질소 분자를 식물이 이용할 수 있는 질화 화합물로 바꿔주기 때문에, 우수한 식물성장 효과를 나타내는 식물영양제 조성물을 제공한다.Therefore, the plant nutritional composition using the clover extract according to the present invention has an excellent plant growth effect because the clover extract containing a large amount of amino acids and nitrogen-fixing bacteria changes nitrogen molecules in the atmosphere into nitrifying compounds that plants can use. Provides a plant nutritional composition representing.
또한, 유기 킬레이트 셀레늄과 아미노산발효부산물액으로 이루어진 그룹에서 선택된 하나 이상이 더 함유되어 식물성장 효과가 더욱 향상될 뿐만 아니라, 친환경적인 식물영양제 조성물을 제공한다.In addition, by further containing one or more selected from the group consisting of organic chelated selenium and amino acid fermentation by-product solution, not only does the plant growth effect further improve, but it also provides an eco-friendly plant nutrient composition.
Claims (6)
상기 토끼풀 추출물은 토끼풀 건조분말 100 중량부에 질량농도가 65 내지 75%인 에탄올 800 내지 1200 중량부를 혼합하고 75 내지 85℃의 온도에서 10 내지 15시간 동안 환류추출하여 제조되고,
상기 토끼풀 추출물 100 중량부 대비 유기 킬레이트 셀레늄 5 내지 10 중량부가 더 함유되는 것을 특징으로 하는 토끼풀 추출물을 이용한 식물영양제 조성물.
A plant nutritional composition containing 40 to 60% by weight of clover extract,
The clover extract is prepared by mixing 800 to 1,200 parts by weight of ethanol with a mass concentration of 65 to 75% with 100 parts by weight of dried clover powder and performing reflux extraction for 10 to 15 hours at a temperature of 75 to 85 ° C.
A plant nutritional composition using a clover extract, characterized in that it contains an additional 5 to 10 parts by weight of organic chelated selenium compared to 100 parts by weight of the clover extract.
상기 유기 킬레이트 셀레늄은 질량농도가 20 내지 50%인 EDTA 수용액 100 중량부에 수산화칼륨 2 내지 15 중량부와 산화셀레늄 2 내지 10 중량부를 차례로 투입하고 반응시켜 제조되는 것을 특징으로 하는 토끼풀 추출물을 이용한 식물영양제 조성물.
In claim 1,
The organic chelated selenium is a plant using clover extract, characterized in that it is prepared by sequentially adding 2 to 15 parts by weight of potassium hydroxide and 2 to 10 parts by weight of selenium oxide to 100 parts by weight of an EDTA aqueous solution with a mass concentration of 20 to 50% and reacting. Nutrient composition.
상기 토끼풀 추출물을 이용한 식물영양제 조성물에는 상기 토끼풀 추출물 100 중량부 대비 아미노산발효부산물액 10 내지 20 중량부가 더 함유되는 것을 특징으로 하는 토끼풀 추출물을 이용한 식물영양제 조성물.
In claim 1,
A plant nutritional composition using the clover extract, characterized in that it contains 10 to 20 parts by weight of amino acid fermentation by-product liquid compared to 100 parts by weight of the clover extract.
상기 아미노산발효부산물액은 아미노산으로서 라이신(lysine)의 생산과정 중에 얻어지는 라이신발효부산물액 또는 글루타민산(glutamic acid)의 생산과정 중에 얻어지는 글루타민산발효부산물액으로 이루어지는 것을 특징으로 하는 토끼풀 추출물을 이용한 식물영양제 조성물.In claim 5,
The amino acid fermentation by-product liquid is a lysine fermentation by-product liquid obtained during the production process of lysine as an amino acid, or a glutamic acid fermentation by-product liquid obtained during the production process of glutamic acid. A plant nutritional composition using a clover extract.
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