KR102576621B1 - Manufacturing method of Fluid Fertilizer Composition promoting Plant Growth - Google Patents

Manufacturing method of Fluid Fertilizer Composition promoting Plant Growth Download PDF

Info

Publication number
KR102576621B1
KR102576621B1 KR1020230018991A KR20230018991A KR102576621B1 KR 102576621 B1 KR102576621 B1 KR 102576621B1 KR 1020230018991 A KR1020230018991 A KR 1020230018991A KR 20230018991 A KR20230018991 A KR 20230018991A KR 102576621 B1 KR102576621 B1 KR 102576621B1
Authority
KR
South Korea
Prior art keywords
weight
parts
extract
hours
leaves
Prior art date
Application number
KR1020230018991A
Other languages
Korean (ko)
Inventor
신은미
김원철
Original Assignee
주식회사 바이오숨
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 주식회사 바이오숨 filed Critical 주식회사 바이오숨
Priority to KR1020230018991A priority Critical patent/KR102576621B1/en
Application granted granted Critical
Publication of KR102576621B1 publication Critical patent/KR102576621B1/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/20Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00Fertilisers characterised by their form
    • C05G5/20Liquid fertilisers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Pest Control & Pesticides (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

본 발명은 식물 생장 촉진용 액상비료조성물의 제조방법에 관한 것으로, 물 100중량부에 녹차잎 10중량부, 은행잎 10중량부, 쑥잎 10중량부 및 뽕잎 10중량부를 가하고 100℃에서 10시간 동안 가열한 후 여과하여 제1 추출액을 수득하는 단계(단계 1); 상기 제1 추출액 60중량%, 말굽버섯 20중량%, 솔잎 10중량% 및 Bacillus firmus bsoom-001 균주(미생물 기탁번호 KCTS 14844BP) 10중량%를 혼합한 후 20~25℃에서 10시간 동안 발효시켜 제1 발효물을 수득하는 단계(단계 2); 물 100중량부에 개똥쑥 10중량부, 질경이 10중량부, 인동초 10중량부 및 모링가잎 10중량부를 가하고 100℃에서 10시간 동안 가열한 후 여과하여 제2 추출액을 수득하는 단계(단계 3); 상기 제2 추출액 60중량%, 느타리버섯 20중량%, 연잎 10중량% 및 Bacillus oryzisoli bsoom-002 균주(미생물 기탁번호 KCTS 14845BP) 10중량%를 혼합한 후 15~20℃에서 10시간 동안 발효시켜 제2 발효물을 수득하는 단계(단계 4); 및 상기 제1 발효물 40중량%, 상기 제2 발효물 40중량% 및 액상효소 20중량부를 가하고 25~30℃에서 10일 동안 발효시켜 식물 생장 촉진용 액상비료조성물을 제조하는 단계(단계 5);를 포함하며, 생육이 우수할 뿐만 아니라 열매의 저장성이 우수한 장점이 있으며, 내한성 및 당도 향상 효과가 우수한 장점이 있다. The present invention relates to a method for producing a liquid fertilizer composition for promoting plant growth, which involves adding 10 parts by weight of green tea leaves, 10 parts by weight of ginkgo leaves, 10 parts by weight of mugwort leaves, and 10 parts by weight of mulberry leaves to 100 parts by weight of water and heating at 100°C for 10 hours. filtering to obtain a first extract (step 1); 60% by weight of the first extract, 20% by weight of horseshoe mushrooms, 10% by weight of pine needles, and 10% by weight of Bacillus firmus bsoom-001 strain (microorganism accession number KCTS 14844BP) were mixed and fermented at 20-25°C for 10 hours. 1 obtaining fermented product (step 2); Adding 10 parts by weight of mugwort, 10 parts by weight of plantain, 10 parts by weight of honeysuckle, and 10 parts by weight of moringa leaves to 100 parts by weight of water, heating at 100°C for 10 hours, and then filtering to obtain a second extract (step 3) ; 60% by weight of the second extract, 20% by weight of oyster mushroom, 10% by weight of lotus leaf, and 10% by weight of Bacillus oryzisoli bsoom-002 strain (microorganism accession number KCTS 14845BP) were mixed and fermented at 15-20°C for 10 hours. 2 obtaining fermented product (step 4); And adding 40% by weight of the first fermentation product, 40% by weight of the second fermentation product, and 20 parts by weight of liquid enzyme and fermenting at 25-30°C for 10 days to prepare a liquid fertilizer composition for promoting plant growth (step 5). It has the advantage of not only excellent growth, but also excellent storage of the fruit, and excellent cold resistance and sugar content improvement effects.

Description

식물 생장 촉진용 액상비료조성물의 제조방법{Manufacturing method of Fluid Fertilizer Composition promoting Plant Growth}{Manufacturing method of Fluid Fertilizer Composition promoting Plant Growth}

본 발명은 식물 생장 촉진용 액상비료조성물의 제조방법에 관한 것으로, 보다 상세하게는 생육이 우수할 뿐만 아니라 열매의 저장성이 우수하며, 내한성 및 당도 향상 효과가 우수한, 식물 생장 촉진용 액상비료조성물의 제조방법에 관한 것이다.The present invention relates to a method of manufacturing a liquid fertilizer composition for plant growth promotion, and more specifically, to a liquid fertilizer composition for plant growth promotion that not only has excellent growth, but also has excellent fruit storage, and has excellent cold resistance and sugar content improvement effects. It is about manufacturing method.

최근 들어 우리나라는 이른 봄 개화기의 급격한 기온하강으로 일교차가 커지고, 여름철 장마기의 불규칙적인 강우와 폭염 등 이상기후로 인하여 농작물 재배시 예측할 수 없는 환경들이 자주 생기게 된다.Recently, in Korea, the daily temperature difference increases due to the rapid drop in temperature during the early spring flowering season, and abnormal climates such as irregular rainfall and heat waves during the summer rainy season often create unpredictable environments when growing crops.

일반적으로 작물은 일시적인 저온과 고온이라도 생육이 거의 멈추게 되고 때문에 작물생육에 피해가 발생하게 된다. 사과, 배, 복숭아 등 과수는 봄에 꽃이 핀 후 수정이 이루어져야 열매가 생기게 되는데 이 시기에 아침 온도가 급격히 하락하거나 낮의 온도가 급격히 올라갈 경우 암술이 온도 차이에 의한 스트레스를 받아 기능을 나타내지 못하여 인공으로 꽃가루 접종을 하여도 수정이 안 되어 착과율이 급격히 저하된다. 반대로, 하우스나 온실의 경우 여름철 온도가 갑자기 올라가는 경우가 생기는데, 농작물은 온도가 올라가면 기공을 통하여 호흡을 많이 하므로 체내의 수분 감소는 물론 호흡에 의한 저장 에너지 소비로 생육과 과실의 발육에 지장을 주게 된다.In general, crop growth almost stops even at temporary low or high temperatures, causing damage to crop growth. Fruit trees such as apples, pears, and peaches must be fertilized after flowering in the spring to produce fruit. If the morning temperature drops sharply or the daytime temperature rises sharply during this period, the pistil is stressed by the temperature difference and cannot function. Even if pollen is artificially inoculated, fertilization does not occur and the fruit set rate rapidly decreases. Conversely, in the case of houses or greenhouses, the temperature may rise suddenly in the summer. Crops respire more through stomata when the temperature rises, which not only reduces moisture in the body but also hinders growth and fruit development due to the consumption of stored energy through respiration. do.

대한민국등록특허공보 제10-1565035호(2015.11.02.)에는 신규한 식물 성장촉진용 액체비료 및 그 제조방법이 개시되어 있다.Republic of Korea Patent Publication No. 10-1565035 (2015.11.02.) discloses a novel liquid fertilizer for plant growth promotion and its manufacturing method.

상기 신규한 식물 성장촉진용 액체비료는 식물의 성장이 촉진되는 장점이 있지만, 열매의 저장성이 미흡한 단점이 있다. The novel liquid fertilizer for plant growth promotion has the advantage of promoting plant growth, but has the disadvantage of insufficient fruit storage.

KRKR 10-1565035 10-1565035 B1B1 2015.11.02.2015.11.02.

본 발명의 목적은 생육이 우수할 뿐만 아니라 열매의 저장성이 우수한, 식물 생장 촉진용 액상비료조성물의 제조방법을 제공하는 것이다. The purpose of the present invention is to provide a method for producing a liquid fertilizer composition for promoting plant growth that not only has excellent growth but also has excellent fruit storage properties.

본 발명의 다른 목적은 내한성 및 당도 향상 효과가 우수한, 식물 생장 촉진용 액상비료조성물의 제조방법을 제공하는 것이다. Another object of the present invention is to provide a method for producing a liquid fertilizer composition for promoting plant growth that has excellent cold resistance and sugar content improvement effects.

상기 목적을 달성하기 위하여 본 발명은 다음과 같은 수단을 제공한다.To achieve the above object, the present invention provides the following means.

본 발명은, 물 100중량부에 녹차잎 10중량부, 은행잎 10중량부, 쑥잎 10중량부 및 뽕잎 10중량부를 가하고 100℃에서 10시간 동안 가열한 후 여과하여 제1 추출액을 수득하는 단계(단계 1); 상기 제1 추출액 60중량%, 말굽버섯 20중량%, 솔잎 10중량% 및 Bacillus firmus bsoom-001 균주(미생물 기탁번호 KCTS 14844BP) 10중량%를 혼합한 후 20~25℃에서 10시간 동안 발효시켜 제1 발효물을 수득하는 단계(단계 2); 물 100중량부에 개똥쑥 10중량부, 질경이 10중량부, 인동초 10중량부 및 모링가잎 10중량부를 가하고 100℃에서 10시간 동안 가열한 후 여과하여 제2 추출액을 수득하는 단계(단계 3); 상기 제2 추출액 60중량%, 느타리버섯 20중량%, 연잎 10중량% 및 Bacillus oryzisoli bsoom-002 균주(미생물 기탁번호 KCTS 14845BP) 10중량%를 혼합한 후 15~20℃에서 10시간 동안 발효시켜 제2 발효물을 수득하는 단계(단계 4); 및 상기 제1 발효물 40중량%, 상기 제2 발효물 40중량% 및 액상효소 20중량부를 가하고 25~30℃에서 10일 동안 발효시켜 식물 생장 촉진용 액상비료조성물을 제조하는 단계(단계 5); 를 포함하는, 식물 생장 촉진용 액상비료조성물의 제조방법을 제공한다.The present invention involves adding 10 parts by weight of green tea leaves, 10 parts by weight of ginkgo leaves, 10 parts by weight of mugwort leaves, and 10 parts by weight of mulberry leaves to 100 parts by weight of water, heating at 100°C for 10 hours, and then filtering to obtain the first extract (step) One); 60% by weight of the first extract, 20% by weight of horseshoe mushrooms, 10% by weight of pine needles, and 10% by weight of Bacillus firmus bsoom-001 strain (microorganism accession number KCTS 14844BP) were mixed and fermented at 20-25°C for 10 hours. 1 obtaining fermented product (step 2); Adding 10 parts by weight of mugwort, 10 parts by weight of plantain, 10 parts by weight of honeysuckle, and 10 parts by weight of moringa leaves to 100 parts by weight of water, heating at 100°C for 10 hours, and then filtering to obtain a second extract (step 3) ; 60% by weight of the second extract, 20% by weight of oyster mushroom, 10% by weight of lotus leaf, and 10% by weight of Bacillus oryzisoli bsoom-002 strain (microorganism accession number KCTS 14845BP) were mixed and fermented at 15-20°C for 10 hours. 2 obtaining fermented product (step 4); And adding 40% by weight of the first fermentation product, 40% by weight of the second fermentation product, and 20 parts by weight of liquid enzyme and fermenting at 25-30°C for 10 days to prepare a liquid fertilizer composition for promoting plant growth (step 5). ; It provides a method for producing a liquid fertilizer composition for promoting plant growth, comprising a.

상기 단계 5에서 상기 액상효소는, 황토지장수 100중량부에 인동꽃봉오리 10중량부, 치커리뿌리 10중량부, 화이트클로버 10중량부, Bacillus firmus bsoom-001 균주(미생물 기탁번호 KCTS 14844BP) 1중량부 및 Bacillus oryzisoli bsoom-002 균주(미생물 기탁번호 KCTS 14845BP) 1중량부를 혼합하고 20~25℃에서 360일 동안 발효시킨 후 여과하여 제조하며, 상기 황토지장수는 바이오세라믹스톤정제수 100중량부에 황토 10중량부 및 생강 5중량부를 3시간 동안 교반 혼합한 후 10시간 동안 침지시켜 제조하며, 상기 바이오세라믹스톤정제수는 물 100중량부에 바이오세라믹스톤 30중량부를 넣고 48시간 동안 방치하여 제조하며, 상기 바이오세라믹스톤은 물 60중량%, 일라이트 20중량%, 펄라이트 10중량% 및 백토 10중량%을 혼련한 후 1,300℃의 온도에서 2시간 동안 소성시켜 제조한다.In step 5, the liquid enzyme is 100 parts by weight of loess, 10 parts by weight of honeysuckle bud, 10 parts by weight of chicory root, 10 parts by weight of white clover, and 1 part by weight of Bacillus firmus bsoom-001 strain (microorganism accession number KCTS 14844BP). and 1 part by weight of Bacillus oryzisoli bsoom-002 strain (microorganism accession number KCTS 14845BP), fermented at 20-25°C for 360 days, and then filtered. It is prepared by stirring and mixing 5 parts by weight of ginger and 5 parts by weight for 3 hours and then soaking for 10 hours. The bioceramic stone purified water is prepared by adding 30 parts by weight of bioceramic stone to 100 parts by weight of water and leaving for 48 hours. The stone is manufactured by mixing 60% by weight of water, 20% by weight of illite, 10% by weight of perlite, and 10% by weight of white clay and then firing at a temperature of 1,300°C for 2 hours.

상기 식물 생장 촉진용 액상비료조성물 100중량부에 내한성 향상제 1중량부를 추가적으로 혼합하되, 상기 내한성 향상제는 해홍나물추출액 50중량%, 나문재추출액 30중량% 및 칠면초추출액 20중량%를 포함하며, 상기 해홍나물추출액은, 해홍나물 1중량부에 90%(v/v) 에탄올 10~15중량부를 첨가하여 교반한 후 환류추출기를 사용하여 80℃에서 4시간 동안 환류 추출한 후 여과하여 제조하며, 상기 나문재추출액은, 나문재 1중량부에 60%(v/v) 에탄올 10~15중량부를 첨가하여 순환추출기를 이용하여 70℃에서 10시간 동안 추출한 후 여과하여 제조하며, 상기 칠면초추출액은 칠면초 100중량부 및 정제수 500중량부를 초음파추출기에 넣고 30℃에서 1시간 동안 추출한 후에 여과하여 제조한다. 1 part by weight of a cold resistance improver is additionally mixed with 100 parts by weight of the liquid fertilizer composition for plant growth promotion, wherein the cold resistance improver includes 50% by weight of Haehongnamul extract, 30% by weight of Namunjae extract, and 20% by weight of Chilmyeoncho extract, and the Haehongnamul extract The extract is prepared by adding 10 to 15 parts by weight of 90% (v/v) ethanol to 1 part by weight of Haehongnamul, stirring, refluxing and extracting at 80°C for 4 hours using a reflux extractor, and then filtering. , It is prepared by adding 10 to 15 parts by weight of 60% (v/v) ethanol to 1 part by weight of Namunjae, extracting it at 70°C for 10 hours using a circulation extractor, and then filtering. The extract is prepared by mixing 100 parts by weight of Chilmyeoncho and 500 parts by weight of purified water. It is prepared by placing a weight portion in an ultrasonic extractor, extracting it at 30°C for 1 hour, and then filtering it.

상기 식물 생장 촉진용 액상비료조성물 100중량부에 당도 향상제 1중량부를 추가적으로 혼합하되, 상기 당도 향상제는 산호초발효액 70중량%, 토탄 20중량%, 클로렐라 5중량% 및 동애등에 5중량%를 포함하며, 상기 산호초발효액의 제조방법은, 산호초 100중량부에 정제수 800중량부를 가하고 100℃에서 20분 동안 가열한 후 여과하여 산호초추출액을 수득하는 단계; 및 상기 산호초추출액 100중량부에 블루베리발효액 30중량부를 가하고 20℃에서 40시간 동안 발효시킨 후 여과하는 단계; 를 포함하며, 상기 블루베리 발효액은 블루베리 60중량% 및 이소말토올리고당 40중량%를 혼합한 후 25℃에서 7일 동안 발효시켜 제조한다.1 part by weight of a sugar content improver is additionally mixed with 100 parts by weight of the liquid fertilizer composition for promoting plant growth, wherein the sugar content improver includes 70% by weight of coral reef fermentation liquid, 20% by weight of peat, 5% by weight of chlorella, and 5% by weight of black soldier soldier, The method for producing the coral reef fermentation liquid includes adding 800 parts by weight of purified water to 100 parts by weight of coral reef, heating at 100° C. for 20 minutes, and then filtering to obtain a coral reef extract; And adding 30 parts by weight of blueberry fermentation liquid to 100 parts by weight of the coral reef extract, fermenting at 20°C for 40 hours, and then filtering; It includes, and the blueberry fermentation broth is prepared by mixing 60% by weight of blueberries and 40% by weight of isomaltooligosaccharide and fermenting at 25°C for 7 days.

본 발명에 따른 식물 생장 촉진용 액상비료조성물의 제조방법은 생육이 우수할 뿐만 아니라 열매의 저장성이 우수한 장점이 있다. The method for producing a liquid fertilizer composition for promoting plant growth according to the present invention has the advantage of not only excellent growth but also excellent storage of fruits.

또한, 본 발명의 식물 생장 촉진용 액상비료조성물의 제조방법은 내한성 및 당도 향상 효과가 우수한 장점이 있다. In addition, the method for producing a liquid fertilizer composition for promoting plant growth of the present invention has the advantage of excellent cold resistance and sugar content improvement effects.

도 1은 실시예 1을 딸기에 엽면시비하여 재배하고, 수확 후 8일 경과 후의 딸기 사진이다.
도 2는 비교에 1을 딸기에 엽면시비하여 재배하고, 수확 후 8일 경과 후의 딸기 사진이다.
Figure 1 is a photograph of strawberries grown by foliar application of Example 1 and 8 days after harvest.
Figure 2 is a photo of strawberries grown by foliar application of No. 1 to strawberries for comparison, 8 days after harvest.

이하, 본 발명을 상세히 설명하면 다음과 같다.Hereinafter, the present invention will be described in detail as follows.

먼저, 본 발명에 따른 식물 생장 촉진용 액상비료조성물의 제조방법을 설명한다.First, a method for producing a liquid fertilizer composition for promoting plant growth according to the present invention will be described.

본 발명의 식물 생장 촉진용 액상비료조성물의 제조방법은,The method for producing the liquid fertilizer composition for plant growth promotion of the present invention is,

물 100중량부에 녹차잎 10중량부, 은행잎 10중량부, 쑥잎 10중량부 및 뽕잎 10중량부를 가하고 100℃에서 10시간 동안 가열한 후 여과하여 제1 추출액을 수득하는 단계(단계 1); Adding 10 parts by weight of green tea leaves, 10 parts by weight of ginkgo leaves, 10 parts by weight of mugwort leaves, and 10 parts by weight of mulberry leaves to 100 parts by weight of water, heating at 100°C for 10 hours, and then filtering to obtain a first extract (step 1);

상기 제1 추출액 60중량%, 말굽버섯 20중량%, 솔잎 10중량% 및 Bacillus firmus bsoom-001 균주(미생물 기탁번호 KCTS 14844BP) 10중량%를 혼합한 후 20~25℃에서 10시간 동안 발효시켜 제1 발효물을 수득하는 단계(단계 2); 60% by weight of the first extract, 20% by weight of horseshoe mushrooms, 10% by weight of pine needles, and 10% by weight of Bacillus firmus bsoom-001 strain (microorganism accession number KCTS 14844BP) were mixed and fermented at 20-25°C for 10 hours. 1 Obtaining fermented product (step 2);

물 100중량부에 개똥쑥 10중량부, 질경이 10중량부, 인동초 10중량부 및 모링가잎 10중량부를 가하고 100℃에서 10시간 동안 가열한 후 여과하여 제2 추출액을 수득하는 단계(단계 3); Adding 10 parts by weight of mugwort, 10 parts by weight of plantain, 10 parts by weight of honeysuckle, and 10 parts by weight of moringa leaves to 100 parts by weight of water, heating at 100°C for 10 hours, and then filtering to obtain a second extract (step 3) ;

상기 제2 추출액 60중량%, 느타리버섯 20중량%, 연잎 10중량% 및 Bacillus oryzisoli bsoom-002 균주(미생물 기탁번호 KCTS 14845BP) 10중량%를 혼합한 후 15~20℃에서 10시간 동안 발효시켜 제2 발효물을 수득하는 단계(단계 4); 및60% by weight of the second extract, 20% by weight of oyster mushroom, 10% by weight of lotus leaf, and 10% by weight of Bacillus oryzisoli bsoom-002 strain (microorganism accession number KCTS 14845BP) were mixed and fermented at 15-20°C for 10 hours. 2 Obtaining fermented product (step 4); and

상기 제1 발효물 40중량%, 상기 제2 발효물 40중량% 및 액상효소 20중량부를 가하고 25~30℃에서 10일 동안 발효시켜 식물 생장 촉진용 액상비료조성물을 제조하는 단계(단계 5);Preparing a liquid fertilizer composition for promoting plant growth by adding 40% by weight of the first fermentation product, 40% by weight of the second fermentation product, and 20 parts by weight of liquid enzyme and fermenting at 25 to 30° C. for 10 days (step 5);

를 포함한다.Includes.

상기 녹차잎은 차나무과에 속하는 상록 소교목인 차나무의 잎을 의미하는 것으로, 상기 차나무의 어린잎을 따서 찌거나 열을 가해 효소의 작용을 억제시키고 말려서 녹차 또는 엽차로 복용하고 있다. 녹차잎에는 카페인, 탄닌, 카테킨(catechin), 비타민, 아미노산, 질소화합물, 엽록소, 탄수화물, 유기산, 무기염류 등이 들어있어 전세계에 걸쳐 기호품으로 널리 복용되며, 또한 녹차에 함유된 카페인은 강심작용, 근육수축작용, 피로 회복, 이뇨작용, 각성작용을 하며, 타닌에 의해 중금속이나 알칼로이드와 같은 독극물 및 단백질의 침전작용을 하므로 해독효과가 있고, 비타민에 의해 신진대사를 촉진시키며 심장, 혈관, 위장 등의 정상적 기능을 유지하는 역할을 한다.The green tea leaves refer to the leaves of the tea tree, an evergreen small tree belonging to the Camellia family. Young leaves of the tea tree are picked, steamed or heated to inhibit the action of enzymes, dried, and taken as green tea or leaf tea. Green tea leaves contain caffeine, tannin, catechin, vitamins, amino acids, nitrogen compounds, chlorophyll, carbohydrates, organic acids, inorganic salts, etc., and are widely taken as a luxury product throughout the world. Additionally, the caffeine contained in green tea has a cardiotonic effect. It has muscle contraction, fatigue recovery, diuretic, and awakening effects. It has a detoxifying effect by precipitating proteins and poisons such as heavy metals and alkaloids through tannin. It promotes metabolism through vitamins and promotes metabolism in the heart, blood vessels, stomach, etc. It plays a role in maintaining the normal function of

상기 은행은 공손수, 행자목이라 하며 잎의 모양이 오리발을 닮았다 하여 압각수라고도 한다. 나무껍질은 회색으로 두껍고 코르크질이며 균열이 생긴다. 가지는 긴 가지와 짧은 가지의 2종류가 있다. 잎은 대부분의 겉씨식물이 침엽인 것과는 달리 은행나무의 잎은 부채꼴이며 중앙에서 2개로 갈라지지만 갈라지지 않는 것과 2개 이상 갈라지는 것 등이 있다. 잎맥은 2개씩 갈라진다. 긴 가지에 달리는 잎은 뭉쳐나고 짧은 가지에서는 총생한다.The above-mentioned ginkgo is called Gongsun tree, Haengja tree, and because the shape of the leaf resembles a duck's foot, it is also called Apgak tree. The bark is gray, thick, corky, and cracked. There are two types of branches: long branches and short branches. Unlike the needle leaves of most gymnosperms, the leaves of ginkgo trees are fan-shaped and split into two in the center, but there are some that are not split and those that are split into two or more. The leaf veins are divided into two. Leaves on long branches grow in clumps, and on short branches they grow in groups.

상기 쑥(Artemisia, Mugwort)은 우리나라 전국 각지의 산과 들에 흔하게 자라며 번식력이 강한 국화과의 다년생 초본이다. 쑥은 음식에 넣어 특유의 맛과 함께 색과 향을 좋게 할 뿐 아니라 미네랄이 풍부하여 알칼리성 식품이며, 클로로필에 의한 항암작용, 혈액순환을 좋게 하여 훈증에 사용되고, 지혈작용, 혈압강하작용, 항알레르기 작용과 함께 항균작용을 갖는 것으로 알려져 있다The above-mentioned mugwort (Artemisia, Mugwort) is a perennial herb of the Asteraceae family that commonly grows in mountains and fields throughout Korea and has strong reproductive capacity. Mugwort is added to food to not only improve its color and aroma along with its unique taste, but is also rich in minerals, making it an alkaline food. It has anti-cancer properties due to chlorophyll, improves blood circulation, is used for fumigation, and has hemostatic, blood pressure-lowering, and anti-allergy properties. It is known to have an antibacterial effect as well as an antibacterial effect.

상기 뽕잎(Mulberry leaves)은 신농본초경, 본초강목, 일본의 오처경, 끽다양생기 및 동의보감에 의하면 각기병, 부종, 당뇨, 탈항 등 약용식물로서의 효과가 기록되어있다. 뽕잎에는 녹차보다 많은 미네랄이 함유되어 있고, 기능성 성분인 루틴(rutin), 쿠에르체틴(quercetin), 쿠에르치트린(quercitrin), 이소린(isoquercitrin) 등과 같은 플라보노이드(flavonoid)와 스테로이드(steroid), 아미노산(amino acid), 비타민(vitamin) 등이 함유되어 있다. 또한, 중추신경계의 억제성 신경전달물질로 혈압상승억제, 식욕 및 포만감 조절 등 중요한 역할을 하는 감마아미노뷰테릴산(γ-aminobutyric acid, GABA)가 비교적 풍부하며, 특히, 알파 글루코시다아제(α-glucosidase)의 활성을 저해하여 당의 흡수를 감소시켜 혈당을 저하시키는 항과혈당(anti-hyperglycemic) 활성이 우수한 1-디옥시노지리마이신(1-deoxynojirimycin, 1-DNJ)과 질소 함유당(N-containing sugar)이 다량으로 함유된 대표적인 천연물이다.The mulberry leaves have been recorded to have medicinal plant effects such as beriberi, edema, diabetes, and prolapse according to Shennong Herbal Medicine, Materia Medica, Japanese Oh Cheo-gyeong, Kkeukda Yangsaengki, and Donguibogam. Mulberry leaves contain more minerals than green tea, and contain functional ingredients such as flavonoids such as rutin, quercetin, quercitrin, and isoquercitrin, and steroids. It contains amino acids and vitamins. In addition, it is relatively rich in γ-aminobutyric acid (GABA), which is an inhibitory neurotransmitter in the central nervous system and plays an important role in suppressing the rise in blood pressure and controlling appetite and satiety. In particular, alpha glucosidase (α- 1-deoxynojirimycin (1-DNJ), which has excellent anti-hyperglycemic activity and nitrogen-containing sugar (N- It is a representative natural product containing a large amount of sugar.

상기 말굽버섯은 구멍장이버섯과(Polyporaceae) 말굽버섯속(Fomes)에 속하는 말굽모양의 매우 단단한 껍질을 가진 버섯으로 민간요법에서는 항종양, 항균, 항산화, 해열, 이뇨 등의 효과로 사용되고 있다. 최근의 연구들은 말굽버섯이 당뇨 개선, 면역조절, 항염증 및 항통증, 항암, 항바이러스 등의 효능을 가지고 있는 것으로 조사되고 있다. The horseshoe mushroom is a mushroom with a horseshoe-shaped, very hard shell belonging to the Fomes genus of the Polyporaceae family. It is used in folk medicine for its antitumor, antibacterial, antioxidant, antipyretic, and diuretic effects. Recent studies have shown that horseshoe mushrooms have effects such as diabetes improvement, immune regulation, anti-inflammatory and anti-pain, anti-cancer, and anti-viral properties.

상기 솔잎은 소나무의 잎을 말하는데, 다량의 아스코르빈산, 비타민 A, B 및 K, 플라보노이드, 안토시안, 탄수화물, 철분, 각종 아미노산이 함유되어 있어 항암, 빈혈, 위궤양 등에 효능이 있는 것으로 알려져 있다. 또한, 피톤치드(phytoncide)는 살균 효과를 가지며, 신진대사 작용을 원활하게 하여 피로 회복에 효과가 있는 것으로 알려져 있다.The pine needles refer to the leaves of the pine tree. They contain a large amount of ascorbic acid, vitamins A, B, and K, flavonoids, anthocyanins, carbohydrates, iron, and various amino acids, and are known to be effective in anticancer, anemia, and stomach ulcers. In addition, phytoncide is known to have a sterilizing effect and is effective in relieving fatigue by facilitating metabolism.

상기 개똥쑥은 쌍떡잎식물 초롱꽃목 국화과의 한해살이 풀로 잔잎쑥, 개땅쑥이라고도 한다. 풀 전체에 털이 없고 특이한 냄새가 난다. 줄기는 녹색으로 가지가 많이 갈라진다. 잎은 어긋나고 2~3회 가늘게 깃꼴로 깊게 갈라진다. 길이 4~7 cm로 모양은 바소꼴이고 겉에 잔털과 선점이 있다. 꽃은 6~9월에 녹황색으로 피며, 작은 두상화가 이삭처럼 달려서 전체가 원추꽃차례를 이룬다. 두상화는 공 모양이고 지름 1.5 cm이다. 총포 조각은 털이 없고 2~3줄로 배열하며, 외포 조각은 긴 타원형으로 녹색이다. 열매는 수과로 길이 약 0.7 mm이다. 한방에서는 발열 감기, 학질, 소아경기, 소화불량, 이질 등의 치료에 사용하는 것으로 알려져 있다.The above artemisia is an annual herb of the dicotyledonous plant Campanula order Asteraceae and is also called artemisia and artemisia. The entire grass is hairless and has a peculiar smell. The stem is green and has many branches. The leaves are alternate and deeply split 2-3 times in a thin, pinnate shape. It is 4-7 cm long, has a lanceolate shape, and has fine hairs and dots on the outside. The flowers bloom in greenish-yellow color from June to September, and the small capillaries grow like spikes, forming a panicle. The capitate is ball-shaped and has a diameter of 1.5 cm. The involucral pieces are hairless and arranged in 2-3 rows, and the outer involucral pieces are long oval and green. The fruit is an achene and is about 0.7 mm long. In oriental medicine, it is known to be used to treat fever, colds, illness, childhood dysentery, indigestion, and dysentery.

상기 질경이(Great plantain)는 질경이과에 속한 여러해살이 풀로 풀밭, 길가에서 흔히 볼 수 있다. 질경이는 무기질, 단백질, 비타민, 당분 등이 다량 함유되어 있어 봄과 여름에 어린 순을 채취하여 다양한 민간식품으로 취식하기도 하며, 씨를 볶아 차로 끓여 먹기도 한다.The great plantain is a perennial grass belonging to the plantain family and is commonly seen in grasslands and roadsides. Plantain contains a large amount of minerals, proteins, vitamins, sugar, etc., so the young shoots are collected in spring and summer and eaten as a variety of folk foods. The seeds are also roasted and boiled into tea.

상기 인동(Lonicera japonica)은 쌍떡잎식물 인동과의 반상록 덩굴식물로 줄기는 적갈색으로 오른쪽으로 감고 올라가고 어린가지는 황갈색의 털이 많고 속이 비어있다. 잎은 마주나고 장타원형으로 예두, 원저이다. 잎자루에는 털이 있고 잎에는 털이 없어지거나 뒷면 일부에만 남는다. 가장자리가 밋밋하지만 뿌리 쪽의 잎은 결각이 나타나기도 한다. 잎겨드랑이에서 입술 모양의 흰색 꽃이 2개씩 피고 꽃받침은 털이 없으며 화관은 끝이 5개로 갈라지고 그 중 1개가 깊게 갈라져 뒤로 말린다. 수술은 5개, 암술은 1개가 있다. 개화시기는 6~7월이다. 둥근 열매는 9~10월에 검은색으로 익는다. 꽃의 색이 흰색에서 노란색으로 변하기 때문에 금은화라고도 한다.The Lonicera japonica is a semi-evergreen vine of the dicotyledonous Lonicera family. The stem is reddish-brown and wraps to the right, and the young branches are yellow-brown and hairy and hollow. The leaves are opposite, oblong, acute, and round-bottomed. There are hairs on the petioles, and the hairs on the leaves disappear or remain only on the back part. The edges are plain, but the leaves near the root sometimes have notches. Two lip-shaped white flowers bloom in each leaf axil, the calyx is hairless, and the corolla is split into five ends, one of which is deeply split and curls backwards. There are 5 stamens and 1 pistil. The flowering period is June to July. The round fruit ripens black in September and October. It is also called honeysuckle because the color of the flower changes from white to yellow.

상기 모링가는 높이가 5~10 m에 이르는 나무로 아시아, 아프리카, 아라비아 등에서 재배된다. 단백질과 비타민이 풍부해 영양가가 높고, 의약적으로 과혈당증과 항염증, 항암 등 다양한 약리작용을 가지고 있으며, 모링가의 꽃, 뿌리, 씨, 잎, 열매에는 다른 식물들에 비해 비타민, 플라보노이드, 아미노산 등과 같은 phytochemical이 풍부한 것으로 알려져 있다. 모링가의 다양한 부위들 중 잎은 β-카로틴, 단백질, 비타민 C, 칼슘 등이 풍부해 항산화제로 이용되며, 피부 염증이나 피부 상처 치유에 효과가 있다는 보고가 있다. 또한 모링가 씨 추출물은 flavonoid와 isothiocyanates, glucosinolates, thiocarbarnates와 같은 생리활성물질을 포함하고 있으며, 간 기능 보호 효과나 암세포의 세푸자살과 증식억제효과 등이 보고되면서 모링가 잎의 우수성이 밝혀진바 있다.The Moringa is a tree reaching 5 to 10 m in height and is cultivated in Asia, Africa, Arabia, etc. It has high nutritional value as it is rich in protein and vitamins, and has various medicinal effects such as hyperglycemia, anti-inflammatory, and anti-cancer. Moringa's flowers, roots, seeds, leaves, and fruits contain vitamins, flavonoids, and amino acids compared to other plants. It is known to be rich in phytochemicals such as Among the various parts of Moringa, the leaves are rich in β-carotene, protein, vitamin C, and calcium, so they are used as antioxidants, and there are reports that they are effective in healing skin inflammation and skin wounds. In addition, Moringa seed extract contains biologically active substances such as flavonoids, isothiocyanates, glucosinolates, and thiocarbarnates, and the superiority of Moringa leaves has been revealed as it has been reported to have effects on protecting liver function and inhibiting apoptosis and proliferation of cancer cells.

상기 느타리버섯(Pleurotus ostreatus)은 주름버섯목 느타리과에 속한다. 버섯은 굴(oyster) 모양으로 생긴 넓은 5-25 cm의 갓을 가졌다. 흰색 부터 회색까지, 또는 짙은 갈색의 색상이다. 참나무나 너도밤나무 같은 활엽수의 고목, 그루터기에 군생하며, 봄에서 가을까지 자라는데, 특히 늦가을에 많이 발생한다. 느타리버섯엔 베타글루칸 등이 풍부해 면역 기능을 높여 암세포 증식을 억제한다. 또한, 느타리버섯은 비타민D2의 모체인 에르고스테롤을 많이 함유하고 있다. 고혈압과 동맥경화의 예방 및 치료에 효과가 뛰어나며 항암치료에도 효과적이다.The oyster mushroom ( Pleurotus ostreatus ) belongs to the Pleurotus ostreatus family. The mushroom has a 5-25 cm wide cap shaped like an oyster. Color ranges from white to gray or dark brown. It grows in groups on old trees and stumps of broad-leaved trees such as oak and beech, and grows from spring to fall, especially in late fall. Oyster mushrooms are rich in beta-glucan, which boosts immunity and suppresses cancer cell proliferation. In addition, oyster mushroom contains a lot of ergosterol, the parent of vitamin D2. It is effective in preventing and treating high blood pressure and arteriosclerosis, and is also effective in chemotherapy.

상기 연은 전국의 연못과 습지에 심어 기르는 여러해살이 풀이다. 세계적으로는 동아시아, 남아시아, 오스트레일리아, 북아메리카 등지에 분포한다. 뿌리줄기는 굵고, 속에 많은 구멍이 있으며, 땅속에서 옆으로 뻗는다. 뿌리 줄기의 마디에서 수염뿌리와 잎이 나온다. 잎은 둥근 방패 모양, 지름 30~90cm, 가운데가 오목하며, 가장자리가 밋밋하다. 잎자루는 길이 1~2m다. 꽃은 7~8월에 물위로 솟은 꽃대 끝에 한 개씩 달리며, 붉은색, 분홍색, 흰색, 지름 10~25cm다. 낮에만 3~4일 동안 계속 핀다. 꽃받침은 4~5장, 녹색, 일찍 떨어진다. 꽃잎은 16~24장, 도란형이다. 수술은 400여 개이고, 꽃밥은 노란색이다. 꽃턱은 해면질, 고깔 모양이며, 겉이 납작하다. 열매는 견과를 닮았으며, 꽃턱의 구멍에 한 개씩 달리고, 타원형, 검게 익는다. 관상용으로 재배하고, 뿌리를 식용으로 흔히 쓰고 뿌리를 얻기 위해 재배한다. 꽃잎과 잎을 차로 만들어 마시기도 하고, 잎을 이용해 찜 요리를 할 때 이용하기도 한다.The lotus is a perennial herb grown in ponds and wetlands across the country. Worldwide, it is distributed in East Asia, South Asia, Australia, and North America. The rhizome is thick, has many holes inside, and extends sideways in the ground. Beard roots and leaves emerge from the nodes of the rhizome. The leaves are round shield-shaped, 30 to 90 cm in diameter, concave in the middle, and have smooth edges. The petiole is 1~2m long. Flowers grow one by one at the end of flower stalks rising above the water from July to August, and are red, pink, white, and 10 to 25 cm in diameter. It blooms only during the day for 3-4 days. There are 4-5 calyxes, green, and fall off early. There are 16 to 24 petals and are obovate. There are about 400 stamens, and the anthers are yellow. The flower ridge is spongy, cone-shaped, and has a flat surface. The fruit resembles a nut, hangs one by one in the hole of the flower stamen, is oval in shape, and ripens black. It is cultivated for ornamental purposes, and the roots are commonly used for food and are cultivated for the roots. The petals and leaves can be made into tea and drunk, and the leaves can also be used when making steamed dishes.

상기 단계 5에서 상기 액상효소는, In step 5, the liquid enzyme is,

황토지장수 100중량부에 인동꽃봉오리 10중량부, 치커리뿌리 10중량부, 화이트클로버 10중량부, Bacillus firmus bsoom-001 균주(미생물 기탁번호 KCTS 14844BP) 1중량부 및 Bacillus oryzisoli bsoom-002 균주(미생물 기탁번호 KCTS 14845BP) 1중량부를 혼합하고 20~25℃에서 360일 동안 발효시킨 후 여과하여 제조한다.For 100 parts by weight of loess, 10 parts by weight of honeysuckle buds, 10 parts by weight of chicory root, 10 parts by weight of white clover, 1 part by weight of Bacillus firmus bsoom-001 strain (microorganism accession number KCTS 14844BP), and Bacillus oryzisoli bsoom-002 strain (microorganism) It is prepared by mixing 1 part by weight (Accession number KCTS 14845BP), fermenting at 20-25°C for 360 days, and then filtering.

상기 황토지장수는 바이오세라믹스톤정제수 100중량부에 황토 10중량부 및 생강 5중량부를 3시간 동안 교반 혼합한 후 10시간 동안 침지시켜 제조한다.The red clay longevity water is prepared by stirring and mixing 10 parts by weight of red clay and 5 parts by weight of ginger in 100 parts by weight of bioceramic stone purified water for 3 hours and then immersing for 10 hours.

상기 황토는 표면이 넓은 벌집형태를 구조로 그 기공 안에 인체에 유익한 원적외선 및 음이온을 저장하고 있으며, 철, 인, 망간, 마그네슘, 황음모 등의 광물, 특히 규소를 많이 함유하고 있어 뼈, 근육, 혈관, 치아, 손톱과 같은 조직을 튼튼하게 해주며, 카탈라아제, 디페놀옥시타아제, 시카라제, 프로테아제 등 수억 마리의 미생물을 포함하고 있어 오염 정화 및 해독제로서 유용한 것이다.The red clay has a honeycomb-shaped structure with a wide surface and stores far-infrared rays and negative ions that are beneficial to the human body within its pores. It contains a lot of minerals such as iron, phosphorus, manganese, magnesium, and sulfur, especially silicon, so it can be used to protect bones, muscles, and bones. It strengthens tissues such as blood vessels, teeth, and nails, and contains hundreds of millions of microorganisms such as catalase, diphenoloxytase, cicolase, and protease, making it useful as a pollution purifier and detoxifier.

상기 생강(Zingiber officinale Roscoe)은 생강과(Zingiberaceae) 식물에 속하는 진지버(Zingiber)속 식물로서, 동남아시아 등지에 광범위하게 분포되어 있으며 민간요법에 따른 약재로 이용되어 왔다. 생강의 성분으로는 전분이 전체의 40~60%를 차지하고, 방향 신미 성분, 수지 단백질, 섬유소, 펜토산, 무기질 등이 있으며, 생강의 매운맛은 진저론(zingeron), 진저롤(gingerol), 쇼가올(shogaol), 디히드로진저롤(dihydrogingerol), 방향성분은 시트랄(citral), 캄펜(camphene) 등 40여 종이 알려져 있다. The ginger (Zingiber officinale Roscoe) is a plant of the genus Zingiber belonging to the Zingiberaceae family of plants, and is widely distributed in Southeast Asia and other countries and has been used as a medicinal herb in folk medicine. Ginger's ingredients include starch, which accounts for 40-60% of the total, aromatic and sour ingredients, resinous proteins, fiber, pentosan, and minerals. The spicy taste of ginger is made up of zingeron, gingerol, and shoga. About 40 species are known, including shogaol, dihydrogingerol, and the aromatic components citral and camphene.

상기 바이오세라믹스톤정제수는 물 100중량부에 바이오세라믹스톤 30중량부를 넣고 48시간 동안 방치하여 제조한다.The bioceramic stone purified water is prepared by adding 30 parts by weight of bioceramic stone to 100 parts by weight of water and leaving it for 48 hours.

상기 바이오세라믹스톤은 물 60중량%, 일라이트 20중량%, 펄라이트 10중량% 및 백토 10중량%을 혼련한 후 1,300℃의 온도에서 2시간 동안 소성시켜 제조한다.The bioceramic stone is manufactured by mixing 60% by weight of water, 20% by weight of illite, 10% by weight of perlite, and 10% by weight of white clay and then firing at a temperature of 1,300°C for 2 hours.

상기 인동꽃봉오리는 금은화라고 하며, 인동과 (Caprifoliaceae)에 속하는 Lonicera japonica Thunb.의 꽃봉오리 또는 막 피기 시작한 꽃을 건조한 약재로 각종 전통 한약 처방에 이용되고 있으며, 체열 조절, 해열, 해독 및 항염 효과가 뛰어난 것으로 알려져 있다.The above-mentioned honeysuckle flower bud is called honeysuckle, and the dried flower buds or flowers that have just begun to bloom of Lonicera japonica Thunb., which belongs to the Caprifoliaceae family, are dried medicinal materials and are used in various traditional herbal medicine prescriptions. They have body heat regulation, antipyretic, detoxification, and anti-inflammatory effects. is known to be excellent.

상기 치커리(Cichorium intybus)는 쌍떡잎식물로 국화과(Asteraceae)에 속하는 다년생초식물이다. 뿌리는 다육질이고 길며, 줄기는 높이가 50~150 cm이고 단단하며 가지가 갈라지고 털이 있다. 뿌리에서 나온 잎은 아래쪽을 향하고 깃꼴로 갈라진다. 갈라진 조각은 밑 부분이 점차 좁아지고 날개와 같은 잎자루가 있으며, 끝에 달린 조각은 크고 옆에 달린 조각은 삼각형이다. 줄기에 달린 잎은 바소꼴의 달걀 모양 또는 바소꼴이고 가장자리가 밋밋하며 뒷면에 털이 있다. 꽃은 7~9월에 하늘색으로 피고 줄기 윗부분 잎겨드랑이와 줄기 끝에 설상화가 두상꽃차례를 이루며 달린다. 총포는 원기둥 모양이고 총포 조각은 2개로 갈라진다. 품종에 따라 흰색 또는 엷은 붉은 색의 꽃도 있다. 치커리는 간장 개선과 시력 회복에 효과가 알려져 있어, 치커리 섭취를 늘릴 경우 장내세균 중 유익한 세균으로 알려진 비피더스균(Bifidobacteria)과 락토바실러스균(Lactobacillus)의 균수가 크게 증가한다고 보고되고 있다.The chicory ( Cichorium intybus ) is a dicotyledonous perennial plant belonging to the Asteraceae family. The root is fleshy and long, and the stem is 50-150 cm high, hard, branched, and hairy. The leaves from the root face downward and are divided into feathers. The split pieces gradually become narrower at the bottom and have wing-like petioles, and the pieces attached to the end are large and the pieces attached to the sides are triangular. The leaves attached to the stem are lancet-shaped egg-shaped or lancet-shaped, the edges are flat, and the back side is hairy. Flowers bloom in sky blue from July to September, and lingual flowers form a capitate inflorescence at the axilla of the upper part of the stem and at the end of the stem. The gun is cylindrical and the gun piece is split into two. Some varieties have white or pale red flowers. Chicory is known to be effective in improving the liver and restoring eyesight, and it has been reported that increasing chicory intake significantly increases the number of Bifidobacteria and Lactobacillus , known as beneficial bacteria among intestinal bacteria.

상기 화이트클로버(Trifolium repense L.)는 단년 내지 다년생 두과 목초로서 직근성이며, 뿌리가 짧고 매우 적은 밑동줄기를 가지고 있다. 포복경 이상부위에서 생산이 가능하고 뿌리마디에서 독립 개체로 번식한다. 일반적으로 각 잎마다 잎 중간 부위에 "V"자 형의 흰 무늬가 있는 것이 특징이다. 꽃은 특별히 흰색이기 때문에 일반적으로 화이트 클로버라고 부르게 되었다. 이러한 화이트 클로버는 약초로 쓰이는 것은 물론 건강식품으로도 활용되고, 잎뿐만 아니라 꽃까지 식용으로 사용할 수 있는 아주 귀중한 산야초이다. 꽃은 튀겨 먹으면 좋고 잎과 함께 샐러드를 만들어 먹으면 상큼한 맛을 즐길 수 있다. 또한 건강식으로 훌륭한 재료가 되는 화이트 클로버를 녹즙과 생식용으로 사용하고 있다. The white clover (Trifolium repense L.) is an annual to perennial leguminous herb that has taproots and has short roots and very few stumps. It can be produced in abnormal parts of the prostrate and reproduces as an independent individual at the root node. In general, each leaf is characterized by a "V" shaped white pattern in the middle of the leaf. Because the flowers are particularly white, they are commonly called white clover. This white clover is not only used as a medicinal herb but also as a health food, and is a very valuable wild and wild herb in which not only the leaves but also the flowers can be used for food. The flowers are good when fried, and you can enjoy a refreshing taste when you make a salad with the leaves. In addition, white clover, which is an excellent ingredient for healthy food, is used for green juice and raw food.

본 발명의 식물 생장 촉진용 액상비료조성물의 제조방법은,The method for producing the liquid fertilizer composition for plant growth promotion of the present invention is,

식물 생장 촉진용 액상비료조성물 100중량부에 내한성 향상제 1중량부를 혼합하는 단계를 추가할 수 있다.A step of mixing 1 part by weight of a cold resistance improver with 100 parts by weight of the liquid fertilizer composition for promoting plant growth can be added.

본 발명은 내한성 향상제를 추가적으로 포함하므로써 내한성을 더욱 향상시킬 수 있는 장점이 있다.The present invention has the advantage of further improving cold resistance by additionally including a cold resistance improving agent.

상기 내한성 향상제는 해홍나물추출액 50중량%, 나문재추출액 30중량% 및 칠면초추출액 20중량%를 포함한다.The cold resistance improver includes 50% by weight of Haehongnamul extract, 30% by weight of Namunjae extract, and 20% by weight of Chilmyeoncho extract.

상기 해홍나물추출액은, 해홍나물 1중량부에 90%(v/v) 에탄올 10~15중량부를 첨가하여 교반한 후 환류추출기를 사용하여 80℃에서 4시간 동안 환류 추출한 후 여과하여 제조한다.The Haehongnamul extract is prepared by adding 10 to 15 parts by weight of 90% (v/v) ethanol to 1 part by weight of Haehongnamul, stirring, refluxing and extracting at 80°C for 4 hours using a reflux extractor, and then filtering.

상기 나문재추출액은, 나문재 1중량부에 60%(v/v) 에탄올 10~15중량부를 첨가하여 순환추출기를 이용하여 70℃에서 10시간 동안 추출한 후 여과하여 제조한다.The Namunjae extract is prepared by adding 10 to 15 parts by weight of 60% (v/v) ethanol to 1 part by weight of Namunjae, extracting it at 70°C for 10 hours using a circular extractor, and then filtering.

상기 칠면초추출액은 칠면초 100중량부 및 정제수 500중량부를 초음파추출기에 넣고 30℃에서 1시간 동안 추출한 후에 여과하여 제조한다. The Chilmyeoncho extract is prepared by placing 100 parts by weight of Chilmyeoncho and 500 parts by weight of purified water in an ultrasonic extractor, extracting at 30°C for 1 hour, and then filtering.

본 발명의 식물 생장 촉진용 액상비료조성물의 제조방법은,The method for producing the liquid fertilizer composition for plant growth promotion of the present invention is,

식물 생장 촉진용 액상비료조성물 100중량부에 당도 향상제 1중량부를 혼합하는 단계를 추가할 수 있다.A step of mixing 1 part by weight of a sugar content improver with 100 parts by weight of the liquid fertilizer composition for promoting plant growth can be added.

본 발명은 당도 향상제를 추가적으로 포함하므로써 열매의 당도를 더욱 향상시킬 수 있는 장점이 있다.The present invention has the advantage of further improving the sugar content of the fruit by additionally including a sugar content improver.

상기 당도 향상제는 산호초발효액 70중량%, 토탄 20중량%, 클로렐라 5중량% 및 동애등에 5중량%를 포함한다.The sugar content improver includes 70% by weight of coral reef fermentation liquid, 20% by weight of peat, 5% by weight of chlorella, and 5% by weight of black soldier fly.

상기 산호초발효액의 제조방법은,The method for producing the coral reef fermentation liquid is,

산호초 100중량부에 정제수 800중량부를 가하고 100℃에서 20분 동안 가열한 후 여과하여 산호초추출액을 수득하는 단계; 및Adding 800 parts by weight of purified water to 100 parts by weight of coral reef, heating at 100°C for 20 minutes, and then filtering to obtain a coral reef extract; and

상기 산호초추출액 100중량부에 블루베리발효액 30중량부를 가하고 20℃에서 40시간 동안 발효시킨 후 여과하는 단계;Adding 30 parts by weight of blueberry fermentation liquid to 100 parts by weight of the coral reef extract, fermenting at 20°C for 40 hours, and then filtering;

를 포함한다.Includes.

상기 블루베리 발효액은 블루베리 60중량% 및 이소말토올리고당 40중량%를 혼합한 후 25℃에서 7일 동안 발효시켜 제조한다. The blueberry fermentation broth is prepared by mixing 60% by weight of blueberries and 40% by weight of isomaltooligosaccharide and fermenting at 25°C for 7 days.

상기 토탄(peat)은 생물의 유체가 불완전 분해된 물질이 퇴적된 것으로, 생성시기에 따라 성분의 차이가 있으며 천연 유기질이다. 토탄은 흡습, 흡수, 흡취성이 좋아서 토지개량제, 미생물활동촉진제 등의 농업 용도와 탈취제, 방부제 등의 의약용, 가정 및 공업용 연료, 비료, 사료제조원료로 쓰일 수 있으며, 특히 식물 생육 활성제의 성분으로서 사용되기에 적합하다. 또한 토탄은 식물에게 필요한 다양한 양분을 포함하고 있으며, 약산성으로 항균성을 가져 박테리아 등의 미생물 번식 억제에도 좋은 효과를 갖는다.The peat is a deposit of incompletely decomposed biological fluids, has different composition depending on the time of formation, and is a natural organic material. Peat has good moisture absorption, absorption, and absorptive properties, so it can be used for agricultural purposes such as land improvement agents and microbial activity promoters, for medicinal purposes such as deodorants and preservatives, and as a raw material for domestic and industrial fuel, fertilizer, and feed manufacturing. In particular, it is an ingredient in plant growth activators. It is suitable to be used as. In addition, peat contains various nutrients necessary for plants, and is slightly acidic, so it has antibacterial properties and is effective in suppressing the growth of microorganisms such as bacteria.

상기 클로렐라(chlorella)는 담수, 해수, 공기, 토양 등 다양한 생태계에서 생존하며 엽록소를 가지고 있어 햇빛으로부터 광합성에 필요한 에너지를 얻고 이산화탄소와 같은 무기물로부터 탄소원을 얻어 독립적으로 성장이 가능한 생물을 일컫는다. 클로렐라는 편모가 없어 운동력이 없고, 세포는 1개의 핵과 컵 모양의 엽록체로 이루어지며, 성장한 세포는 특이한 세포 분열을 통해 빠르게 증식하고, 보통 4개의 딸세포가 내생포자를 형성하고 있으며, 피레노이도사(Pyrenoidosa), 불가리스(Bulgaris), 엘립소이데아(Ellipsoidea) 등 현재 약 10여종이 알려져 있다. 이러한 클로렐라는 5대 영양소를 비롯한 40여 가지의 영양소를 모두 함유하고 있을 뿐만 아니라, 광합성 능력도 우수하여 엽록소가 일반 채소류보다 10배나 많고, 현대인에게 결핍되어 있는 미네랄(mineral), 비타민 등이 다량 함유되어 있어 신체 영양의 균형을 맞추어 주고, 면역력 강화, 중금속 배출, 다이옥신 흡수 억제 등에 대한 뛰어난 효과가 있다. 게다가, 클로렐라는 알칼리성 식품으로서 육류나 곡류 등의 과다섭취로 인한 산성 체질을 알칼리성 체질로 변화시켜 주어 체내의 이온 밸런스의 균형을 유지해 주는 유용한 기능을 한다.The chlorella refers to an organism that survives in various ecosystems such as freshwater, seawater, air, and soil, and has chlorophyll, so it obtains the energy needed for photosynthesis from sunlight and obtains carbon sources from inorganic substances such as carbon dioxide and can grow independently. Chlorella has no flagella, so it has no motile power. The cell consists of one nucleus and a cup-shaped chloroplast. The grown cell proliferates rapidly through unique cell division, and usually four daughter cells form endospores. Pyrenoidosa (pyrenoidosa) About 10 species are currently known, including Pyrenoidosa, Bulgaris, and Ellipsoidea. Chlorella not only contains all 40 nutrients, including the five major nutrients, but also has excellent photosynthetic ability, so it contains 10 times more chlorophyll than ordinary vegetables, and contains a large amount of minerals and vitamins that modern people are deficient in. It balances the body's nutrition and has excellent effects on strengthening immunity, excreting heavy metals, and inhibiting dioxin absorption. In addition, chlorella is an alkaline food and has a useful function in maintaining the ion balance in the body by changing the acidic constitution caused by excessive intake of meat or grains into an alkaline constitution.

상기 동애등에는 절지동물 곤충강 파리목 동애등에과에 속하는 곤충이다. 동애등에와 같은 파리의 유충은 아르기닌, 메티오닌, 라이신 등과 같은 필수아미노산의 좋은 공급원으로 알려져 있는데 이러한 아미노산 함량은 일반적으로 대두박보다 높고 어분과 같은 수준으로 알려져 있다. The black soldier fly is an insect belonging to the arthropod insect class Diptera and the black soldier fly family. Fly larvae such as black soldier flies are known to be a good source of essential amino acids such as arginine, methionine, and lysine, and the content of these amino acids is generally known to be higher than that of soybean meal and at the same level as fish meal.

본 발명에 따른 식물 생장 촉진용 액상비료조성물의 제조방법은 생육이 우수할 뿐만 아니라 열매의 저장성이 우수한 장점이 있다. The method for producing a liquid fertilizer composition for promoting plant growth according to the present invention has the advantage of not only excellent growth but also excellent storage of fruits.

또한, 본 발명의 식물 생장 촉진용 액상비료조성물의 제조방법은 내한성 및 당도 향상 효과가 우수한 장점이 있다. In addition, the method for producing a liquid fertilizer composition for promoting plant growth of the present invention has the advantage of excellent cold resistance and sugar content improvement effects.

이하, 실시 예를 통하여 본 발명의 구성 및 효과를 더욱 상세히 설명하고자 한다. 이들 실시 예는 오로지 본 발명을 예시하기 위한 것일 뿐 본 발명의 범위가 이들 실시 예에 의해 제한되는 것은 아니다. Hereinafter, the configuration and effects of the present invention will be described in more detail through examples. These examples are only for illustrating the present invention and the scope of the present invention is not limited by these examples.

물 100중량부에 녹차잎 10중량부, 은행잎 10중량부, 쑥잎 10중량부 및 뽕잎 10중량부를 가하고 100℃에서 10시간 동안 가열한 후 여과하여 제1 추출액을 수득하였다. 상기 제1 추출액 60중량%, 말굽버섯 20중량%, 솔잎 10중량% 및 Bacillus firmus bsoom-001 균주(미생물 기탁번호 KCTS 14844BP) 10중량%를 혼합한 후 25℃에서 10시간 동안 발효시켜 제1 발효물을 수득하였다. 물 100중량부에 개똥쑥 10중량부, 질경이 10중량부, 인동초 10중량부 및 모링가잎 10중량부를 가하고 100℃에서 10시간 동안 가열한 후 여과하여 제2 추출액을 수득하였다. 상기 제2 추출액 60중량%, 느타리버섯 20중량%, 연잎 10중량% 및 Bacillus oryzisoli bsoom-002 균주(미생물 기탁번호 KCTS 14845BP) 10중량%를 혼합한 후 15~20℃에서 10시간 동안 발효시켜 제2 발효물을 수득하였다. 상기 제1 발효물 40중량%, 상기 제2 발효물 40중량% 및 액상효소 20중량부를 가하고 30℃에서 10일 동안 발효시켜 식물 생장 촉진용 액상비료조성물을 제조하였다. 10 parts by weight of green tea leaves, 10 parts by weight of ginkgo leaves, 10 parts by weight of mugwort leaves, and 10 parts by weight of mulberry leaves were added to 100 parts by weight of water, heated at 100°C for 10 hours, and filtered to obtain the first extract. 60% by weight of the first extract, 20% by weight of horseshoe mushrooms, 10% by weight of pine needles, and 10% by weight of Bacillus firmus bsoom-001 strain (microorganism accession number KCTS 14844BP) were mixed and then fermented at 25°C for 10 hours to perform the first fermentation. water was obtained. 10 parts by weight of mugwort, 10 parts by weight of plantain, 10 parts by weight of honeysuckle, and 10 parts by weight of moringa leaves were added to 100 parts by weight of water, heated at 100°C for 10 hours, and filtered to obtain a second extract. 60% by weight of the second extract, 20% by weight of oyster mushroom, 10% by weight of lotus leaf, and 10% by weight of Bacillus oryzisoli bsoom-002 strain (microorganism accession number KCTS 14845BP) were mixed and fermented at 15-20°C for 10 hours. 2 fermented products were obtained. 40% by weight of the first fermentation product, 40% by weight of the second fermentation product, and 20 parts by weight of liquid enzyme were added and fermented at 30° C. for 10 days to prepare a liquid fertilizer composition for promoting plant growth.

상기 액상효소는 황토지장수 100중량부에 인동꽃봉오리 10중량부, 치커리뿌리 10중량부, 화이트클로버 10중량부, Bacillus firmus bsoom-001 균주(미생물 기탁번호 KCTS 14844BP) 1중량부 및 Bacillus oryzisoli bsoom-002 균주(미생물 기탁번호 KCTS 14845BP) 1중량부를 혼합하고 25℃에서 360일 동안 발효시킨 후 여과하여 제조하였다. 상기 황토지장수는 바이오세라믹스톤정제수 100중량부에 황토 10중량부 및 생강 5중량부를 3시간 동안 교반 혼합한 후 10시간 동안 침지시켜 제조하였다. 상기 바이오세라믹스톤정제수는 물 100중량부에 바이오세라믹스톤 30중량부를 넣고 48시간 동안 방치하여 제조하였다. 상기 바이오세라믹스톤은 물 60중량%, 일라이트 20중량%, 펄라이트 10중량% 및 백토 10중량%을 혼련한 후 1,300℃의 온도에서 2시간 동안 소성시켜 제조하였다.The liquid enzyme contains 10 parts by weight of honeysuckle flower buds, 10 parts by weight of chicory root, 10 parts by weight of white clover, 1 part by weight of Bacillus firmus bsoom-001 strain (microorganism accession number KCTS 14844BP), and Bacillus oryzisoli bsoom-000 parts by weight of loess water. It was prepared by mixing 1 part by weight of strain 002 (microorganism accession number KCTS 14845BP), fermenting at 25°C for 360 days, and then filtering. The red clay longevity water was prepared by stirring and mixing 10 parts by weight of red clay and 5 parts by weight of ginger in 100 parts by weight of bioceramic stone purified water for 3 hours and then immersing for 10 hours. The bioceramic stone purified water was prepared by adding 30 parts by weight of bioceramic stone to 100 parts by weight of water and leaving it for 48 hours. The bioceramic stone was manufactured by mixing 60% by weight of water, 20% by weight of illite, 10% by weight of perlite, and 10% by weight of white clay and then firing at a temperature of 1,300°C for 2 hours.

[비교예 1][Comparative Example 1]

감태, 천초 및 파래를 각각 물로 세척하여 이물질을 제거한 후, 상온에서 건조하였다. 농도가 1.0×108 cfu/g인 락토코커스 락티스 및 유당을 1:99의 중량비로 혼합한 후 건조하였다. 건조된 감태, 천초 및 파래를 중량비 1:1:1로 혼합한 해초 20 중량부, 락토코커스 락티스 혼합물 0.5 중량부, 당밀 10 중량부 및 물 69.5 중량부를 교반기에 넣어 혼합한 후, pH가 4 이하가 될 때까지 상온에서 20일 동안 발효시킨 뒤 정제하여 해초 발효물을 제조하였다. 토탄추출물 5 중량부, 상기 제조된 해초 발효물 25 중량부 및 물 70 중량부를 혼합하여 식물성장촉진제를 제조하였다. 상기 토탄 추출물은 해저 600m 정도에서 채취한 부식토인 해저 퇴적물에서 추출한 것을 사용하였다.Gamtae, seaweed, and green seaweed were each washed with water to remove foreign substances, and then dried at room temperature. Lactococcus lactis and lactose having a concentration of 1.0×10 8 cfu/g were mixed in a weight ratio of 1:99 and then dried. 20 parts by weight of seaweed mixed with dried Ecklonia cava, seaweed and green seaweed in a weight ratio of 1:1:1, 0.5 parts by weight of Lactococcus lactis mixture, 10 parts by weight of molasses and 69.5 parts by weight of water were mixed in a stirrer and the pH was 4. After fermenting at room temperature for 20 days until the following, a fermented seaweed was prepared by purification. A plant growth promoter was prepared by mixing 5 parts by weight of peat extract, 25 parts by weight of the seaweed fermentation product prepared above, and 70 parts by weight of water. The peat extract was used that was extracted from seabed sediments, which are humic soils collected from about 600 m below sea level.

[실험예 1][Experimental Example 1]

실시예 1의 2000배 희석액 및 비교예 1의 2000배 희석액을 딸기에 2회 엽면시비하였다. 딸기 수확후 8일 경과 후의 사진을 도 1(실시예 1) 및 도 2(비교예 1)에 나타내었다.The 2000-fold dilution of Example 1 and the 2000-fold dilution of Comparative Example 1 were applied to strawberries twice as foliar applications. Photographs taken 8 days after harvesting strawberries are shown in Figure 1 (Example 1) and Figure 2 (Comparative Example 1).

도 1 및 도 2를 대비하면, 비교예 1을 엽면시비한 딸기는 8일 경과 후에 딸기 씨가 튀어 나오는데 비하여 실시예 1을 엽면시비한 딸기는 8일 경과 후에도 원형을 유지하는 것을 알 수 있다.Comparing Figures 1 and 2, it can be seen that the strawberry seeds popped out after 8 days of foliar application of Comparative Example 1, while the strawberry seeds of the strawberry foliar application of Example 1 maintained their original shape even after 8 days.

[실험예 2][Experimental Example 2]

실시예 1의 500배 희석액 및 비교예 1의 500배 희석액을 마에 1회 살포하였다. 그 결과 마의 크기 및 무게를 표 1에 나타내었다.The 500-fold dilution of Example 1 and the 500-fold dilution of Comparative Example 1 were sprayed on the hemp once. As a result, the size and weight of the yam are shown in Table 1.

크기size 무게weight 실시예 1Example 1 48㎝48cm 1987g1987g 비교예 1Comparative Example 1 36㎝36cm 1692g1692g

표 1에 의하면, 실시예 1의 500배 희석액이 살포된 마는 비교예 1의 500배 희석액이 살포된 마에 비하여 크기 및 무게가 성장한 것을 알 수 있다.According to Table 1, it can be seen that the hemp sprayed with the 500-fold dilution of Example 1 grew in size and weight compared to the hemp sprayed with the 500-fold dilution of Comparative Example 1.

실시예 1에서 제조한 식물 생장 촉진용 액상비료조성물 100중량부에 내한성 향상제 1중량부를 혼합하였다.1 part by weight of a cold resistance improver was mixed with 100 parts by weight of the liquid fertilizer composition for plant growth promotion prepared in Example 1.

상기 내한성 향상제는 해홍나물추출액 50중량%, 나문재추출액 30중량% 및 칠면초추출액 20중량%를 혼합하여 제조하였다. 상기 해홍나물추출액은, 해홍나물 1중량부에 90%(v/v) 에탄올 10중량부를 첨가하여 교반한 후 환류추출기를 사용하여 80℃에서 4시간 동안 환류 추출한 후 여과하여 제조하였다. 상기 나문재추출액은, 나문재 1중량부에 60%(v/v) 에탄올 10중량부를 첨가하여 순환추출기를 이용하여 70℃에서 10시간 동안 추출한 후 여과하여 제조하였다. 상기 칠면초추출액은 칠면초 100중량부 및 정제수 500중량부를 초음파추출기에 넣고 30℃에서 1시간 동안 추출한 후에 여과하여 제조하였다. The cold resistance improver was prepared by mixing 50% by weight of Haehongnamul extract, 30% by weight of Namunjae extract, and 20% by weight of Chilmyeoncho extract. The Haehongnamul extract was prepared by adding 10 parts by weight of 90% (v/v) ethanol to 1 part by weight of Haehongnamul, stirring, then refluxing and extracting at 80°C for 4 hours using a reflux extractor, and then filtering. The Namunjae extract was prepared by adding 10 parts by weight of 60% (v/v) ethanol to 1 part by weight of Namunjae, extracting it at 70°C for 10 hours using a circular extractor, and then filtering. The Chilmyeoncho extract was prepared by placing 100 parts by weight of Chilmyeoncho and 500 parts by weight of purified water in an ultrasonic extractor, extracting at 30°C for 1 hour, and then filtering.

[실험예 3][Experimental Example 3]

실시예 1, 실시예 2 및 비교예 1의 조성물을 고추 유묘에 1회 엽면 처리하고, 5℃ 저온 조건의 생장 챔버(Growth chamber)에서 5일 동안 방치한 후, 다시 1회 재처리하고 저온 상태를 유지하면서 방치하였다. 이때, 총 10일 동안 저온 상태를 유지하면서 동일한 수돗물을 1일 1회 관수하고 방치하였다. 상기 처리 조건은 저온조건에서 수분이 투입되면 토양 내 온도는 더욱 더 낮아지므로 식물이 스트레스를 더 많이 받게 된다The compositions of Example 1, Example 2, and Comparative Example 1 were treated once on the foliage of pepper seedlings, left in a growth chamber at a low temperature of 5°C for 5 days, and then retreated once again and grown at a low temperature. was maintained and left unattended. At this time, the same tap water was applied once a day while maintaining low temperature for a total of 10 days and left to stand. The above treatment conditions are such that when moisture is added under low temperature conditions, the temperature in the soil becomes even lower, so the plants experience more stress.

구분division 생엽율(%)Fresh leaf rate (%) 수분 함량(%)Moisture content (%) 비교예 1Comparative Example 1 10.110.1 100100 실시예 1Example 1 60.160.1 117117 실시예 2Example 2 65.965.9 128128

표 2에 의하면, 실시예 1 및 실시예 2의 식물 생장 촉진용 액상비료조성물은 비교예 1의 식물성장촉진제에 비하여 저온기에도 생육이 우수한 것을 알 수 있다.According to Table 2, it can be seen that the liquid fertilizer composition for plant growth promotion of Examples 1 and 2 has excellent growth even at low temperatures compared to the plant growth promoter of Comparative Example 1.

실시예 1에서 제조한 식물 생장 촉진용 액상비료조성물 100중량부에 당도 향상제 1중량부를 혼합하였다.1 part by weight of a sugar content improver was mixed with 100 parts by weight of the liquid fertilizer composition for plant growth promotion prepared in Example 1.

상기 당도 향상제는 산호초발효액 70중량%, 토탄 20중량%, 클로렐라 5중량% 및 동애등에 5중량%를 혼합하여 제조하였다. 상기 산호초발효액은, 산호초 100중량부에 정제수 800중량부를 가하고 100℃에서 20분 동안 가열한 후 여과하여 산호초추출액을 수득하고, 상기 산호초추출액 100중량부에 블루베리발효액 30중량부를 가하고 20℃에서 40시간 동안 발효시킨 후 여과하여 제조하였다. 상기 블루베리 발효액은 블루베리 60중량% 및 이소말토올리고당 40중량%를 혼합한 후 25℃에서 7일 동안 발효시켜 제조하였다. The sweetness improver was prepared by mixing 70% by weight of coral reef fermentation liquid, 20% by weight of peat, 5% by weight of chlorella, and 5% by weight of black soldier fly. The coral reef fermentation liquid was obtained by adding 800 parts by weight of purified water to 100 parts by weight of the coral reef, heating at 100°C for 20 minutes, and then filtering to obtain a coral reef extract. 30 parts by weight of the blueberry fermentation liquid was added to 100 parts by weight of the coral reef extract, and 40% by weight at 20°C. It was prepared by fermenting for a period of time and then filtering. The blueberry fermentation broth was prepared by mixing 60% by weight of blueberries and 40% by weight of isomaltooligosaccharide and fermenting at 25°C for 7 days.

[실험예 4][Experimental Example 4]

참외(품종명: 오복)을 대상으로 실시예 1, 실시예 3 및 비교예 1의 조성물에 대한 당도 향상 효과를 관찰하였다. 참외는 비닐하우스에 정식 후 각 실험 조건별로 살포시설을 이용하여 10일 간격으로 3회에 걸쳐 실시예 1, 실시예 3 및 비교예 1의 조성물을 살포하였고 30일 경과 시점까지 각 실험군에 1㎡ 토양에 대해 0.1 ~ 3g/㎡ 범위의 비율로 같은 양을 각 조건의 하우스에 살포 공급하였다. 과의 개수 및 과의 당도를 측정하여 결과를 표 3에 나타냈다. The effect of improving the sweetness of the compositions of Example 1, Example 3, and Comparative Example 1 was observed on melon (variety name: Obok). After the melons were planted in the greenhouse, the compositions of Examples 1, 3, and Comparative Example 1 were sprayed three times at 10-day intervals using spray facilities for each experimental condition, and 1 m2 of water was applied to each experimental group until 30 days had passed. The same amount of soil was sprayed and supplied to the houses under each condition at a rate ranging from 0.1 to 3 g/m2. The number of fruits and the sugar content of the fruits were measured and the results are shown in Table 3.

구분division 과의 개수(개)Number of families (units) 과의 당도(Brix)Sugar content (Brix) 비교예 1Comparative Example 1 1818 1414 실시예 1Example 1 2121 1616 실시예 3Example 3 2222 1818

표 3에 의하면, 실시예 1 및 실시예 3의 식물 생장 촉진용 액상비료조성물은 비교예 1의 식물성장촉진제에 비하여 당도 향상 효과가 우수한 것을 알 수 있다.According to Table 3, it can be seen that the liquid fertilizer compositions for plant growth promotion of Examples 1 and 3 have a superior sugar content improvement effect compared to the plant growth promoter of Comparative Example 1.

한국생명공학연구원 생물자원센터Korea Research Institute of Bioscience and Biotechnology Biological Resources Center KCTC14844BPKCTC14844BP 2022010620220106 한국생명공학연구원 생물자원센터Korea Research Institute of Bioscience and Biotechnology Biological Resources Center KCTC14845BPKCTC14845BP 2022010620220106

Claims (4)

물 100중량부에 녹차잎 10중량부, 은행잎 10중량부, 쑥잎 10중량부 및 뽕잎 10중량부를 가하고 100℃에서 10시간 동안 가열한 후 여과하여 제1 추출액을 수득하는 단계(단계 1);
상기 제1 추출액 60중량%, 말굽버섯 20중량%, 솔잎 10중량% 및 Bacillus firmus bsoom-001 균주(미생물 기탁번호 KCTC 14844BP) 10중량%를 혼합한 후 20~25℃에서 10시간 동안 발효시켜 제1 발효물을 수득하는 단계(단계 2);
물 100중량부에 개똥쑥 10중량부, 질경이 10중량부, 인동초 10중량부 및 모링가잎 10중량부를 가하고 100℃에서 10시간 동안 가열한 후 여과하여 제2 추출액을 수득하는 단계(단계 3);
상기 제2 추출액 60중량%, 느타리버섯 20중량%, 연잎 10중량% 및 Bacillus oryzisoli bsoom-002 균주(미생물 기탁번호 KCTC 14845BP) 10중량%를 혼합한 후 15~20℃에서 10시간 동안 발효시켜 제2 발효물을 수득하는 단계(단계 4); 및
상기 제1 발효물 40중량%, 상기 제2 발효물 40중량% 및 액상효소 20중량부를 가하고 25~30℃에서 10일 동안 발효시켜 식물 생장 촉진용 액상비료조성물을 제조하는 단계(단계 5);
를 포함하되,
상기 단계 5에서 상기 액상효소는,
황토지장수 100중량부에 인동꽃봉오리 10중량부, 치커리뿌리 10중량부, 화이트클로버 10중량부, Bacillus firmus bsoom-001 균주(미생물 기탁번호 KCTC 14844BP) 1중량부 및 Bacillus oryzisoli bsoom-002 균주(미생물 기탁번호 KCTC 14845BP) 1중량부를 혼합하고 20~25℃에서 360일 동안 발효시킨 후 여과하여 제조하며,
상기 황토지장수는 바이오세라믹스톤정제수 100중량부에 황토 10중량부 및 생강 5중량부를 3시간 동안 교반 혼합한 후 10시간 동안 침지시켜 제조하며,
상기 바이오세라믹스톤정제수는 물 100중량부에 바이오세라믹스톤 30중량부를 넣고 48시간 동안 방치하여 제조하며,
상기 바이오세라믹스톤은 물 60중량%, 일라이트 20중량%, 펄라이트 10중량% 및 백토 10중량%을 혼련한 후 1,300℃의 온도에서 2시간 동안 소성시켜 제조하는,
식물 생장 촉진용 액상비료조성물의 제조방법.
Adding 10 parts by weight of green tea leaves, 10 parts by weight of ginkgo leaves, 10 parts by weight of mugwort leaves, and 10 parts by weight of mulberry leaves to 100 parts by weight of water, heating at 100°C for 10 hours, and then filtering to obtain a first extract (step 1);
60% by weight of the first extract, 20% by weight of horseshoe mushrooms, 10% by weight of pine needles, and 10% by weight of Bacillus firmus bsoom-001 strain (microorganism accession number KCTC 14844BP) were mixed and fermented at 20-25°C for 10 hours. 1 obtaining fermented product (step 2);
Adding 10 parts by weight of mugwort, 10 parts by weight of plantain, 10 parts by weight of honeysuckle, and 10 parts by weight of moringa leaves to 100 parts by weight of water, heating at 100°C for 10 hours, and then filtering to obtain a second extract (step 3) ;
60% by weight of the second extract, 20% by weight of oyster mushroom, 10% by weight of lotus leaf, and 10% by weight of Bacillus oryzisoli bsoom-002 strain (microorganism accession number KCTC 14845BP) were mixed and fermented at 15-20°C for 10 hours. 2 obtaining fermented product (step 4); and
Preparing a liquid fertilizer composition for promoting plant growth by adding 40% by weight of the first fermentation product, 40% by weight of the second fermentation product, and 20 parts by weight of liquid enzyme and fermenting at 25 to 30° C. for 10 days (step 5);
Including,
In step 5, the liquid enzyme,
For 100 parts by weight of loess, 10 parts by weight of honeysuckle buds, 10 parts by weight of chicory root, 10 parts by weight of white clover, 1 part by weight of Bacillus firmus bsoom-001 strain (microorganism accession number KCTC 14844BP), and Bacillus oryzisoli bsoom-002 strain (microorganism) It is manufactured by mixing 1 part by weight (Accession number KCTC 14845BP), fermenting at 20~25℃ for 360 days, and then filtering.
The red clay longevity water is manufactured by stirring and mixing 10 parts by weight of red clay and 5 parts by weight of ginger in 100 parts by weight of bioceramic stone purified water for 3 hours and then immersing for 10 hours,
The bioceramic stone purified water is prepared by adding 30 parts by weight of bioceramic stone to 100 parts by weight of water and leaving it for 48 hours.
The bioceramic stone is manufactured by mixing 60% by weight of water, 20% by weight of illite, 10% by weight of perlite, and 10% by weight of white clay and then fired at a temperature of 1,300°C for 2 hours.
Method for producing a liquid fertilizer composition for promoting plant growth.
삭제delete 제 1항에 있어서
상기 식물 생장 촉진용 액상비료조성물 100중량부에 내한성 향상제 1중량부를 추가적으로 혼합하되,
상기 내한성 향상제는 해홍나물추출액 50중량%, 나문재추출액 30중량% 및 칠면초추출액 20중량%를 포함하며,
상기 해홍나물추출액은, 해홍나물 1중량부에 90%(v/v) 에탄올 10~15중량부를 첨가하여 교반한 후 환류추출기를 사용하여 80℃에서 4시간 동안 환류 추출한 후 여과하여 제조하며,
상기 나문재추출액은, 나문재 1중량부에 60%(v/v) 에탄올 10~15중량부를 첨가하여 순환추출기를 이용하여 70℃에서 10시간 동안 추출한 후 여과하여 제조하며,
상기 칠면초추출액은 칠면초 100중량부 및 정제수 500중량부를 초음파추출기에 넣고 30℃에서 1시간 동안 추출한 후에 여과하여 제조하는,
식물 생장 촉진용 액상비료조성물의 제조방법.
According to claim 1
1 part by weight of a cold resistance improver is additionally mixed with 100 parts by weight of the liquid fertilizer composition for promoting plant growth,
The cold resistance improver includes 50% by weight of Haehongnamul extract, 30% by weight of Namunjae extract, and 20% by weight of Chilmyeoncho extract,
The Haehongnamul extract is prepared by adding 10 to 15 parts by weight of 90% (v/v) ethanol to 1 part by weight of Haehongnamul, stirring, refluxing and extracting at 80°C for 4 hours using a reflux extractor, and then filtering,
The Namunjae extract is prepared by adding 10 to 15 parts by weight of 60% (v/v) ethanol to 1 part by weight of Namunjae, extracting it at 70°C for 10 hours using a circular extractor, and then filtering,
The Chilmyeoncho extract is prepared by placing 100 parts by weight of Chilmyeoncho and 500 parts by weight of purified water in an ultrasonic extractor, extracting at 30°C for 1 hour, and then filtering.
Method for producing a liquid fertilizer composition for promoting plant growth.
제 1항에 있어서,
상기 식물 생장 촉진용 액상비료조성물 100중량부에 당도 향상제 1중량부를 추가적으로 혼합하되,
상기 당도 향상제는 산호초발효액 70중량%, 토탄 20중량%, 클로렐라 5중량% 및 동애등에 5중량%를 포함하며,
상기 산호초발효액의 제조방법은,
산호초 100중량부에 정제수 800중량부를 가하고 100℃에서 20분 동안 가열한 후 여과하여 산호초추출액을 수득하는 단계; 및
상기 산호초추출액 100중량부에 블루베리발효액 30중량부를 가하고 20℃에서 40시간 동안 발효시킨 후 여과하는 단계;
를 포함하며,
상기 블루베리 발효액은 블루베리 60중량% 및 이소말토올리고당 40중량%를 혼합한 후 25℃에서 7일 동안 발효시켜 제조하는,
식물 생장 촉진용 액상비료조성물의 제조방법.
According to claim 1,
1 part by weight of the sugar content improver is additionally mixed with 100 parts by weight of the liquid fertilizer composition for promoting plant growth,
The sugar content improver includes 70% by weight of coral reef fermented liquid, 20% by weight of peat, 5% by weight of chlorella, and 5% by weight of Dongae,
The method for producing the coral reef fermentation liquid,
Adding 800 parts by weight of purified water to 100 parts by weight of coral reef, heating at 100°C for 20 minutes, and then filtering to obtain a coral reef extract; and
Adding 30 parts by weight of blueberry fermentation liquid to 100 parts by weight of the coral reef extract, fermenting at 20°C for 40 hours, and then filtering;
Including,
The blueberry fermentation broth is prepared by mixing 60% by weight of blueberries and 40% by weight of isomaltooligosaccharide and fermenting at 25°C for 7 days.
Method for producing a liquid fertilizer composition for promoting plant growth.
KR1020230018991A 2023-02-13 2023-02-13 Manufacturing method of Fluid Fertilizer Composition promoting Plant Growth KR102576621B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1020230018991A KR102576621B1 (en) 2023-02-13 2023-02-13 Manufacturing method of Fluid Fertilizer Composition promoting Plant Growth

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1020230018991A KR102576621B1 (en) 2023-02-13 2023-02-13 Manufacturing method of Fluid Fertilizer Composition promoting Plant Growth

Publications (1)

Publication Number Publication Date
KR102576621B1 true KR102576621B1 (en) 2023-09-08

Family

ID=88018312

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020230018991A KR102576621B1 (en) 2023-02-13 2023-02-13 Manufacturing method of Fluid Fertilizer Composition promoting Plant Growth

Country Status (1)

Country Link
KR (1) KR102576621B1 (en)

Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20050080811A (en) * 2004-02-11 2005-08-18 주식회사 넬바이오텍 Method for decrease odor and accelation decomposition from animal manure and its liquid fertilizer tank
JP2010208893A (en) * 2009-03-10 2010-09-24 Koichi Seki Organic fertilizer and method of manufacturing organic fertilizer
KR20110012399A (en) * 2009-07-30 2011-02-09 양동흠 The salicomia herbaceal vegetable and the cultivation method for salicomia herbaceal
JP2013099394A (en) * 2011-11-08 2013-05-23 Fujiwara Sangyo Kk Temporary toilet
KR20140127940A (en) * 2013-04-25 2014-11-05 박연노 Offensive reduction sprinkler
KR101565035B1 (en) 2013-11-14 2015-11-02 한경철 Novel liquid fertilizer for promotion the growth of plant, manufacturing method thereof and cultivation method using the same
KR20160062885A (en) * 2014-11-26 2016-06-03 김성동 Composition for Facilitating of Mold or Actinomycetes Activity and Method for Eliminating or Decreasing of a Bad Smell
KR20180086963A (en) * 2017-01-24 2018-08-01 왕한호 A toilet bowl
KR20190056774A (en) * 2017-11-17 2019-05-27 유한회사 축산냄새연구소 Composition comprising Saccharomyces, Bacillus, and Lactobacillus for removing a bad smell and method for removing a bad smell using the same
KR102424401B1 (en) * 2022-02-16 2022-07-22 주식회사 바이오숨 Manufacturing method of Composition for Sewage Odor reduction and Organic Material Reduction of Water Treatment Plant and Composition manufactured by same and Treatment Method of Sewage using therof
KR20220114347A (en) * 2021-02-08 2022-08-17 이문우 Liquid fertilizer manufacturing method of organic natural hormones for plant activation and promoting plant growth

Patent Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20050080811A (en) * 2004-02-11 2005-08-18 주식회사 넬바이오텍 Method for decrease odor and accelation decomposition from animal manure and its liquid fertilizer tank
JP2010208893A (en) * 2009-03-10 2010-09-24 Koichi Seki Organic fertilizer and method of manufacturing organic fertilizer
KR20110012399A (en) * 2009-07-30 2011-02-09 양동흠 The salicomia herbaceal vegetable and the cultivation method for salicomia herbaceal
JP2013099394A (en) * 2011-11-08 2013-05-23 Fujiwara Sangyo Kk Temporary toilet
KR20140127940A (en) * 2013-04-25 2014-11-05 박연노 Offensive reduction sprinkler
KR101565035B1 (en) 2013-11-14 2015-11-02 한경철 Novel liquid fertilizer for promotion the growth of plant, manufacturing method thereof and cultivation method using the same
KR20160062885A (en) * 2014-11-26 2016-06-03 김성동 Composition for Facilitating of Mold or Actinomycetes Activity and Method for Eliminating or Decreasing of a Bad Smell
KR20180086963A (en) * 2017-01-24 2018-08-01 왕한호 A toilet bowl
KR20190056774A (en) * 2017-11-17 2019-05-27 유한회사 축산냄새연구소 Composition comprising Saccharomyces, Bacillus, and Lactobacillus for removing a bad smell and method for removing a bad smell using the same
KR20220114347A (en) * 2021-02-08 2022-08-17 이문우 Liquid fertilizer manufacturing method of organic natural hormones for plant activation and promoting plant growth
KR102424401B1 (en) * 2022-02-16 2022-07-22 주식회사 바이오숨 Manufacturing method of Composition for Sewage Odor reduction and Organic Material Reduction of Water Treatment Plant and Composition manufactured by same and Treatment Method of Sewage using therof

Similar Documents

Publication Publication Date Title
KR20140112778A (en) Method for producing breathing fermented solution of wild plants
Kumar et al. Cactus pear: Cultivation and uses
CN104472727A (en) Making process for Guanyin bean curd
KR101926153B1 (en) Method for producing Makgeolli using fermented solution of wild plants
KR101400021B1 (en) The method for cultivation various mushrooms using natural substances
Bashir et al. Moringa oleifera, a potential miracle tree; a review
KR101024429B1 (en) Composition for feed for poultry comprising oriental herb
KR20130139121A (en) Growing method of artemisia annua and manufacturing method of tea
KR19990015959A (en) How to grow bean sprouts using herbal extracts
KR102070788B1 (en) Nutritional Supplements for Preventing Cold Weather Damage and Manufacturing method thereof
KR100768471B1 (en) Manufacturing method of beverage extract using pine cone and pine cone
KR102576621B1 (en) Manufacturing method of Fluid Fertilizer Composition promoting Plant Growth
Deans et al. Artemisia dracunculus
KR101324466B1 (en) Manufacturing method of functional edible oil
KR102305141B1 (en) Manufacturing method of instant food salad and instant food salad manufactured by using the same
KR101998801B1 (en) Plant Nutrient Composition comprising Shell Extract, Coral reef Extract and Microbial Agent and Manufacturing method thereof
CN110627573A (en) Method for preparing special fertilizer for improving soil hardening by using tea milli as raw material
KR102016609B1 (en) Nutritional Supplements for Grape Cultivation comprising Coffee Resudual Product, Charcoal Powder and Microbial Agent and Manufacturing method thereof
CN110692426A (en) Making method of toona sinensis tea
KR101799815B1 (en) Manufacturing method of Dolsan mustard Kimchi containing Saposhnikovia divaricata Schiskin and mustard Kimchi thereof
KR101613841B1 (en) The low sodium soybean taste containing Phellinus linteus and the manufacturing method of it
KR102415159B1 (en) Blended Honey Manufacturing System
CN107568271A (en) Plant extracts with disease-prevention and insecticide and raising nutrient content of plant
KR102006969B1 (en) Nutritional Supplements for Apple Cultivation comprising Shell Extract, Egg Shell Powder and Microbial Agent and Manufacturing method thereof
Rahim et al. Collection and conservation of Aroids in Bangladesh Agriculture University Germplasm Centre (GPC)

Legal Events

Date Code Title Description
E701 Decision to grant or registration of patent right
GRNT Written decision to grant