KR102548473B1 - Fullerene-indigo pulverata levis complex, a preparation method thereof and a use of the same - Google Patents

Fullerene-indigo pulverata levis complex, a preparation method thereof and a use of the same Download PDF

Info

Publication number
KR102548473B1
KR102548473B1 KR1020210053168A KR20210053168A KR102548473B1 KR 102548473 B1 KR102548473 B1 KR 102548473B1 KR 1020210053168 A KR1020210053168 A KR 1020210053168A KR 20210053168 A KR20210053168 A KR 20210053168A KR 102548473 B1 KR102548473 B1 KR 102548473B1
Authority
KR
South Korea
Prior art keywords
fullerene
complex
inflammatory
extract
cheongdae
Prior art date
Application number
KR1020210053168A
Other languages
Korean (ko)
Other versions
KR20220146198A (en
Inventor
박민희
이영란
이유정
심주영
이동현
김기윤
함재호
Original Assignee
한국세라믹기술원
주식회사 네프랩
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 한국세라믹기술원, 주식회사 네프랩 filed Critical 한국세라믹기술원
Priority to KR1020210053168A priority Critical patent/KR102548473B1/en
Publication of KR20220146198A publication Critical patent/KR20220146198A/en
Application granted granted Critical
Publication of KR102548473B1 publication Critical patent/KR102548473B1/en

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/19—Cosmetics or similar toiletry preparations characterised by the composition containing inorganic ingredients
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
    • A61K8/0241—Containing particulates characterized by their shape and/or structure
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
    • A61K8/73—Polysaccharides
    • A61K8/731—Cellulose; Quaternized cellulose derivatives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
    • A61K2800/57—Compounds covalently linked to a(n inert) carrier molecule, e.g. conjugates, pro-fragrances
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
    • A61K2800/59—Mixtures
    • A61K2800/592—Mixtures of compounds complementing their respective functions
    • A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/70—Biological properties of the composition as a whole
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/81—Preparation or application process involves irradiation
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/82—Preparation or application process involves sonication or ultrasonication

Abstract

본 발명은 풀러린 및 청대 추출물이 고분자 매트릭스에 포집된 나노입자 형태의 풀러린-청대 복합체에 관한 것으로, 상기 풀러린-청대 복합체는 분산성이 우수하여 제품의 제형화에 용이할 뿐 아니라 제품의 안정성이 우수하며, 풀러린 및 청대에 의한 항염증 효과 또한 현저하여 항염증용 소재로 이용가능하다.The present invention relates to a fullerene-green belt complex in the form of nanoparticles in which fullerene and a blueberry extract are collected in a polymer matrix, and the fullerene-green belt complex has excellent dispersibility, so it is easy to formulate a product and has excellent product stability. In addition, the anti-inflammatory effect by fullerene and cheongdae is also remarkable, so it can be used as an anti-inflammatory material.

Description

풀러린-청대 복합체, 이의 제조방법 및 이의 용도{FULLERENE-INDIGO PULVERATA LEVIS COMPLEX, A PREPARATION METHOD THEREOF AND A USE OF THE SAME}Fullerene-Chengdu complex, its preparation method and its use

본 발명은 풀러린-청대 복합체, 이의 제조방법 및 이의 용도에 관한 것으로, 더욱 구체적으로는 풀러린, 청대 추출물 및 고분자를 포함하고, 풀러린 및 청대 추출물이 고분자 매트릭스에 포집된 나노입자 형태의 풀러린-청대 복합체에 관한 것이다.The present invention relates to a fullerene-Cheondae complex, a method for preparing the same, and a use thereof, and more specifically, to a fullerene-Cheondae complex in the form of nanoparticles containing fullerene, a Cheongdae extract and a polymer, and in which the fullerene and the Cheongdae extract are collected in a polymer matrix. It is about.

염증은 상처나 질병에 반응하는 인체의 면역 반응으로, 자외선이나 활성산소, 자유라디칼 등의 산화적 스트레스 등이 염증성 인자를 활성화시켜 각종 질병 및 피부의 노화를 일으킨다. 염증을 소실시키기 위한 항염제로 이용되는 물질로는 비스테로이드계로 플루페나믹산(Flufenamic acid), 이부프로펜(Ibuprofen), 벤지다민(Benzydamine) 또는 인도메타신(Indomethacin) 등이 있고 스테로이드계통으로 프레드니솔론(Prednisolone) 또는 덱사메타손 (Dexamethasone) 등이 있으나, 이들 물질은 피부에 대한 안전성의 문제로 사용량의 제한이 있거나, 효과가 미미하여 실질적으로 염증 완화 효과를 기대할 수 없는 문제점이 있으므로, 안전하면서도 우수한 염증 효과를 나타낼 수 있는 소재의 개발이 필요하다.Inflammation is an immune response of the human body in response to wounds or diseases, and oxidative stress such as ultraviolet rays, active oxygen, and free radicals activate inflammatory factors to cause various diseases and aging of the skin. Substances used as anti-inflammatory agents to reduce inflammation include flufenamic acid, ibuprofen, benzydamine, or indomethacin in non-steroidal systems, and prednisolone in steroid systems. or dexamethasone, but these substances have a problem in that the amount of use is limited due to safety issues on the skin, or the effect is insignificant and the effect of relieving inflammation cannot be expected. material development is required.

풀러린(fullerene)은 일반적으로 60개의 탄소가 축구공 또는 구형 모양으로 결합되어 중공을 형성하고 있는 흑연이나 다이아몬드와 같은 탄소 동소체이다. 그러나, 풀러린은 상기 흑연이나 다이아몬드와는 다른 구조적 형태를 가지기 때문에 제3의 탄소 동소체로 불린다. 풀러린은 C60 내지 C80 또는 이보다 큰 탄소수(C82, C90, C94, C96)를 갖기도 한다. 풀러린은 상기와 같은 탄소 고리 배열 및 중공상의 독특한 구조 때문에 매우 단단한 성질을 가지고 있으며, 우수한 물리적 및 화학적 성질을 갖는다.Fullerene is an allotrope of carbon, such as graphite or diamond, in which 60 carbon atoms are generally bonded together in a soccer ball or sphere shape to form a hollow. However, since fullerene has a structural form different from that of graphite or diamond, it is called a third allotrope of carbon. Fullerene may have C 60 to C 80 or more carbon atoms (C 82 , C 90 , C 94 , C 96 ). Fullerene has a very hard property and excellent physical and chemical properties because of the unique structure of the carbon ring arrangement and hollow shape as described above.

풀러린은 화학적 합성을 통해서 생산할 수도 있지만, 풀러린 성분을 함유하고 있는 천연광물로부터 전기화학적방법 또는 극성용매추출방법 등을 이용하여 추출하는 천연적 합성법을 통해서도 생산할 수 있다. 그러나, 천연적 합성법을 위해 이용되는 천연광물에는 탄소, 탄소동소체, 풀러린과 같은 유기물 외에도 규소, 니켈, 철 등의 무기물을 포함하고 있어 추출 시 이러한 무기물의 일부가 풀러린과 함께 추출되어 풀러린의 순도가 떨어지는 문제점이 있다. 풀러린은 고체이기 때문에 용매에 대한 용해성이나 분산성이 매우 낮기 때문에 화장료 조성물 또는 피부외용제 조성물로서 이용하기 위해서는 풀러린 입자를 개질하거나 가용화 또는 분산하는 공정을 장시간 동안 진행해야하므로, 제조 공정적 측면에서 효율적이지 못하다. 그 뿐만 아니라 풀러린은 고체이기 때문에 풀러린이 나노 사이즈를 갖는 입자라고 하더라도 용매 내에 100% 용해되기 어려우며, 용해되었다 하더라도 일정 시간이 지나면 풀러린 입자가 침전물 형태로 변형되거나 응집되며, 이러한 침전물 및 응질물의 형성은 화장품 제형에도 악영향을 미친다. 상기 응집된 풀러린 입자는 소비자의 사용감을 저하시키고, 미관상 불쾌감을 주며, 풀러린 입자의 표면적 감소에 의해 화장품의 특정 기능성을 저하시키는 문제점이 발생한다. 따라서, 풀러린을 포함하는 화장품, 피부외용제의 안정성을 개선하기 위한 새로운 방법이 요구되고 있다.Fullerene can be produced through chemical synthesis, but it can also be produced through natural synthesis of extraction using an electrochemical method or a polar solvent extraction method from a natural mineral containing a fullerene component. However, natural minerals used for the natural synthesis method include organic substances such as carbon, carbon allotrope, and fullerene as well as inorganic substances such as silicon, nickel, and iron. There is a problem with falling. Since fullerene is a solid, its solubility or dispersibility in solvents is very low. Therefore, the process of modifying, solubilizing, or dispersing fullerene particles must be carried out for a long time in order to be used as a cosmetic composition or an external composition for skin, so it is not efficient in terms of the manufacturing process. Can not do it. In addition, since fullerene is a solid, it is difficult to dissolve 100% in a solvent even if it is a nano-sized particle, and even if it is dissolved, after a certain period of time, the fullerene particle transforms into a precipitate or aggregates. It also adversely affects cosmetic formulations. The agglomerated fullerene particles cause a problem of deteriorating the consumer's feeling of use, giving aesthetic discomfort, and deteriorating specific functionality of cosmetics due to a decrease in the surface area of the fullerene particles. Therefore, a new method for improving the stability of cosmetics and external skin preparations containing fullerene is required.

종래에 풀러린을 화장료 조성물로 이용하는 기술이 공개된 바 있다. 한국등록특허 제1633302호는 천연 플러렌을 함유한 화장료 조성물 및 그 제조방법에 관한 것으로, 광물로부터 추출된 탄소성분을 붕소 존재하여 열처리 함으로써 회(ash) 성분은 적고, 중공 구조(공극율)를 최대한 확보한 천연 플러렌을 제조하고 이를 화장료 조성물로서 이용하여 우수한 항산화성, 살균성 및 담체적 기능을 갖도록 한 것을 기술적 특징으로 한다. 그러나 상기 기술은 화장료 조성물로서 풀러린을 그대로 이용하고 있기 때문에 분산성이 낮아 제형 안정성의 문제는 여전히 존재하며, 본원발명과 같이 제형 안정성을 개선하기 위한 구성 또는 항염증 효과에 대한 구성은 전혀 개시되어 있지 않다.Conventionally, a technique of using fullerene as a cosmetic composition has been disclosed. Korean Patent Registration No. 1633302 relates to a cosmetic composition containing natural fullerene and a method for manufacturing the same. Carbon components extracted from minerals are heat-treated in the presence of boron to minimize ash and maximize hollow structure (porosity). It is technically characterized by manufacturing natural fullerene and using it as a cosmetic composition to have excellent antioxidant, bactericidal and carrier functions. However, since the technology uses fullerene as it is as a cosmetic composition, the problem of formulation stability still exists due to low dispersibility, and configurations for improving formulation stability or anti-inflammatory effects are not disclosed at all, as in the present invention. not.

한편, 청대(Indigo Pulverata Levis)는 마디풀과(여뀌과, Polygonaceae) 마디풀속(여뀌속, Polygonum)에 속한 쪽/요람(P. tinctorium) 또는 판람속(Baphicacanthus) 마람(Baphicacanthus Cusia (Nees) Bremek.)의 잎을 발효시켜 얻은 가루이다. 한방에서 예로부터 청대의 효능으로서 여러 가지가 알려져 있는데, 특히 청열해독(淸熱解毒), 살충(殺蟲), 양혈 산종(凉血散腫) 등의 효능 있는 것으로 알려져 있다. 또한 약리 작용으로 항균 작용, 항염증 작용, 항건선 활성, 항궤양성 대장염 및 항산화 작용을 하는 것으로 알려져 있다. 특히 청대는 항염증에 효과가 있는 것으로 알려져 있어 피부 염증을 개선하고 치료하기 위한 화장품, 피부외용제의 원료로 이용하기 위한 관심이 증가하고 있다. 그러나 청대와 같은 천연 원료를 유효성분으로 포함하는 천연 화장품의 경우, 온도, 빛 및 산도 등에 민감하여 보관과 개발에 한계가 있기 때문에 안정성을 확보하기 위한 기술 개발이 필요한 실정이다.On the other hand, Indigo Pulverata Levis is P. tinctorium or Baphicacanthus Cusia (Nees) Bremek. belonging to Polygonum of Polygonaceae. It is a powder obtained by fermenting the leaves of In oriental medicine, various medicinal properties have been known since ancient times, and in particular, it is known to have effects such as clearing heat and detoxification, insecticide, and yang blood acidity. It is also known to have antibacterial action, anti-inflammatory action, anti-psoriasis activity, anti-ulcerative colitis and antioxidant action as pharmacological actions. In particular, Cheongdae is known to be effective in anti-inflammatory, so interest in using it as a raw material for cosmetics and external skin preparations to improve and treat skin inflammation is increasing. However, in the case of natural cosmetics containing natural raw materials such as blueberry as an active ingredient, there is a limitation in storage and development due to sensitivity to temperature, light and acidity, so technology development to secure stability is required.

이에, 본 발명자들은 상기 종래기술들의 문제점들을 극복하기 위하여 예의 연구노력한 결과, 풀러린 및 청대 추출물이 고분자 매트릭스에 포집된 나노입자 형태의 풀러린-청대 복합체의 경우, 분산성이 우수하여 제품의 제형화에 용이할 뿐 아니라 제품의 안정성이 우수하며, 풀러린 및 청대에 의한 항염증 효과 또한 현저하여 항염증용 소재로 이용가능함을 확인하고, 본 발명을 완성하게 되었다.Accordingly, the present inventors have made intensive research efforts to overcome the problems of the prior art, and as a result, in the case of the fullerene-Cheondae complex in the form of nanoparticles in which fullerene and Cheongdae extract are collected in a polymer matrix, the dispersibility is excellent, which is useful in product formulation. It was not only easy, but also the stability of the product was excellent, and the anti-inflammatory effect by fullerene and cheongdae was also remarkable, confirming that it could be used as an anti-inflammatory material, and the present invention was completed.

KRKR 10-163330210-1633302 B1B1

따라서, 본 발명의 주된 목적은 분산성이 우수하여 제품의 제형화에 용이할 뿐 아니라 제품의 안정성이 우수하며, 풀러린 및 청대에 의한 항염증 효과 또한 현저하여 항염증용 소재로 이용가능한 풀러린-청대 복합체를 제공하는 데 있다.Therefore, the main object of the present invention is that it is easy to formulate a product due to excellent dispersibility, and the stability of the product is excellent, and the anti-inflammatory effect of fullerene and green belt is also remarkable and can be used as an anti-inflammatory material. It is to provide a complex.

본 발명의 다른 목적은 상기 풀러린-청대 복합체를 제조하기 위한 제조방법을 제공하는 데 있다.Another object of the present invention is to provide a manufacturing method for preparing the fullerene-green belt complex.

본 발명의 다른 목적은 상기 풀러린-청대 복합체를 유효성분으로 포함하는 항염증용 화장료 조성물 및 피부 외용제 조성물을 제공하는 데 있다.Another object of the present invention is to provide a cosmetic composition for anti-inflammatory and a composition for external application for skin comprising the fullerene-green complex as an active ingredient.

본 발명의 한 양태에 따르면, 본 발명은 풀러린, 청대 추출물 및 고분자를 포함하는 풀러린-청대 복합체를 제공한다.According to one aspect of the present invention, the present invention provides a fullerene-green belt complex comprising fullerene, a green belt extract, and a polymer.

본 발명에 따른 풀러린-청대 복합체에 있어서, 상기 풀러린-청대 복합체는 풀러린 및 청대 추출물이 고분자 매트릭스에 포집된 나노입자 형태인 것을 특징으로 한다.In the fullerene-green belt complex according to the present invention, the fullerene-green belt complex is characterized in that it is in the form of nanoparticles in which fullerene and blue belt extract are trapped in a polymer matrix.

풀러린과 청대 추출물은 항산화, 항균, 항염증 등 다양한 효과로 인해 화장품 또는 외용제의 원료로 이용가능한 원료이나, 풀러린의 낮은 분산성과 외부 환경에 의한 청대 추출물의 불안정성 때문에 제품화하는데 어려움이 있었다. 이에 본 발명자들은 풀러린 및 청대 추출물을 고분자 매트릭스에 포함시킨 나노입자 형태의 풀러린-청대 복합체의 경우 제품의 제형화에 용이할 뿐 아니라 제품의 안정성이 우수하며 풀러린 및 청대에 의한 항염증 효과 또한 현저함을 확인하였다.Fullerene and Cheongdae extract are raw materials that can be used as raw materials for cosmetics or external preparations due to their various effects such as antioxidant, antibacterial, and anti-inflammatory, but it is difficult to commercialize them due to the low dispersibility of fullerene and the instability of Cheongdae extract due to the external environment. Accordingly, the present inventors found that the fullerene-Cheondae complex in the form of nanoparticles containing fullerene and Cheongdae extract in a polymer matrix is not only easy to formulate a product, but also has excellent product stability, and the anti-inflammatory effect of fullerene and Cheongdae is also remarkable. confirmed.

본 발명에 따른 풀러린-청대 복합체에 있어서, 상기 풀러린은 자외선으로 산화처리된 풀러린인 것을 특징으로 한다.In the fullerene-blue complex according to the present invention, the fullerene is characterized in that it is a fullerene oxidized with ultraviolet light.

본 발명의 일 실험예에 따르면, 천연 풀러린에 UV를 조사할 경우 풀러린이 산화되어 에테르기(C-O-C)의 산소 피크를 확인할 수 있었다. 이러한 결과는 풀러린의 산화에 의해 산소가 노출됨으로써 풀러린의 물 분산성을 증가시킬 수 있음을 의미한다(실험예 1 참조).According to an experimental example of the present invention, when natural fullerene is irradiated with UV, the fullerene is oxidized and the oxygen peak of the ether group (C-O-C) can be confirmed. This result means that the water dispersibility of fullerene can be increased by exposing oxygen by oxidation of fullerene (see Experimental Example 1).

본 발명에 따른 풀러린-청대 복합체에 있어서, 상기 고분자는 풀러린 및 청대 추출물을 포집할 수 있고 분산성을 향상시킬 수 있는 어떠한 고분자도 이용 가능하며, 바람직하게는 폴리에틸렌글리콜(polyethyleneglycol; PEG), 폴리이소프로필아크릴아미드(poly N-isopropylacrylamide; PNIPAM), 폴리메틸아크릴레이트(polymethylacrylate; PMA), 폴리메틸메타아크릴레이트(polymethylmetacrylate;PMMA), 폴리아크릴아미드, 폴리아크릴릭에시드(polyacrylic acide; PAA), 폴리메타아크릴릭에시드(polymethacrylic acid; PMAA), 폴리비닐피롤리돈(polyvinylpyrrolidone; PVP), 폴리에틸렌옥사이드(PEO), 폴리비닐알코올(polyvinylalcohol; PVA), 셀룰로오스(Cellulose), 콜라겐(Collagen), 알지네이트(Alginate), 키토산(Chitosan), 및 이들의 조합으로 구성된 군으로부터 선택되는 하나 이상의 친수성 고분자, 또는 폴리에틸렌글리콜 (polyethyleneglycol; PEG), 폴리이소프로필아크릴아미드(poly Nisopropylacrylamide; PNIPAM), 폴리메틸아크릴레이트(polymethylacrylate; PMA), 폴리메틸메타아크릴레이트(polymethylmetacrylate; PMMA), 폴리아크릴아미드(polyacrylamide; PAM), 폴리아크릴릭에시드(polyacrylic acide; PAA), 폴리메타아크릴릭에시드(polymethacrylic acid; PMAA), 폴리비닐피롤리돈(poly vinylpyrrolidone; PVP), 폴리비닐알코올(polyvinylalcohol; PVA), 셀룰로오스(Cellulose), 폴리에틸렌옥사이드(PEO), 콜라겐(Collagen), 알지네이트(Alginate), 키토산(Chitosan) 및 이들의 조합으로 이루어진 군에서 선택되는 것을 포함하는 선형, 블록, 그래프트 또는 덴드리머 친수성 고분자 블록, 및 이들의 조합으로 구성된 군으로부터 선택되는 하나 이상의 양친매성 고분자인 것을 특징으로 한다.In the fullerene-green bamboo complex according to the present invention, any polymer capable of collecting fullerene and green bamboo extract and improving dispersibility can be used as the polymer, preferably polyethylene glycol (PEG), polyiso Poly N-isopropylacrylamide (PNIPAM), polymethylacrylate (PMA), polymethylmethacrylate (PMMA), polyacrylamide, polyacrylic acide (PAA), polymethacrylic Acid (polymethacrylic acid; PMAA), polyvinylpyrrolidone (PVP), polyethylene oxide (PEO), polyvinylalcohol (PVA), cellulose, collagen, alginate, chitosan (Chitosan), and at least one hydrophilic polymer selected from the group consisting of combinations thereof, or polyethylene glycol (polyethyleneglycol; PEG), poly isopropylacrylamide (PNIPAM), polymethylacrylate (polymethylacrylate; PMA), polymethylmethacrylate (PMMA), polyacrylamide (PAM), polyacrylic acide (PAA), polymethacrylic acid (PMAA), polyvinylpyrrolidone; PVP), polyvinylalcohol (PVA), cellulose, polyethylene oxide (PEO), collagen, alginate, chitosan, and combinations thereof. It is characterized in that it is at least one amphiphilic polymer selected from the group consisting of linear, block, graft or dendrimer hydrophilic polymer blocks, and combinations thereof.

본 발명에 따른 풀러린-청대 복합체는 입자크기가 360nm, 분산도가 0.16으로, 나노입자 사이즈를 갖고 분산도가 우수하므로, 화장품 또는 외용제의 소재로 이용 시 제형화에 용이하다.The fullerene-blue complex according to the present invention has a particle size of 360 nm and a degree of dispersion of 0.16, has a nanoparticle size and excellent degree of dispersion, so it is easy to formulate when used as a material for cosmetics or external preparations.

그 결과 도 3에서 확인할 수 있듯이, 입자크기와 분산도는 각각 약 360nm, 0.16 으로, 풀러린-청대 복합체가 균일한 크기로 제조되었으며, 물에서의 분산성도 우수한 것을 확인하였다.As a result, as can be seen in FIG. 3, the particle size and dispersity were about 360 nm and 0.16, respectively, and the fullerene-blue complex was prepared with a uniform size, and it was confirmed that the dispersibility in water was excellent.

본 발명의 다른 한 양태에 따르면, 본 발명은 고분자와 청대 추출물을 혼합하여 제1 용액을 준비하는 제1 단계, 천연풀러린에 자외선을 조사하여 산화시켜 산화 풀러린을 수득하는 제2 단계, 상기 제2 단계에서 제조된 산화 풀러린과 청대 추출물을 혼합하여 제2 용액을 준비하는 제3 단계, 상기 제1 용액 및 제2 용액을 혼합한 혼합물을 교반 및 초음파 처리하는 제4 단계, 및 상기 제4 단계의 혼합물을 원심분리한 후 상등액을 제거하는 단계를 포함하는 풀러린-청대 복합체의 제조방법을 제공한다.According to another aspect of the present invention, the present invention provides a first step of preparing a first solution by mixing a polymer and a blueberry extract, a second step of oxidizing natural fullerene by irradiating it with ultraviolet rays to obtain oxidized fullerene, and the second step of obtaining oxidized fullerene. A third step of preparing a second solution by mixing the oxidized fullerene prepared in the step and the Cheongdae extract, a fourth step of stirring and ultrasonicating the mixture of the first solution and the second solution, and the fourth step of Provided is a method for producing a fullerene-green complex comprising the step of centrifuging the mixture and removing the supernatant.

본 발명에 따른 풀러린-청대 복합체 추출물의 제조방법은 고분자 또는 천연풀러린과 청대 추출물을 혼합할 때, 용매를 이용하지 않고 청대 추출물을 이용하여 직접 혼합함으로써 각 성분의 유효성분이 용매에 의해 희석되어 항염증 효과가 저하되지 않을 수 있다.In the method for producing the fullerene-Cheondae complex extract according to the present invention, when mixing the polymer or natural fullerene and the Cheongdae extract, the active ingredients of each component are diluted with the solvent by directly mixing the extract without using a solvent, thereby reducing the anti-inflammatory effect. effect may not be diminished.

본 발명의 다른 한 양태에 따르면, 본 발명은 풀러린, 청대 추출물 및 고분자를 포함하는 풀러린-청대 복합체를 유효성분으로 포함하는 항염증용 화장료 조성물을 제공한다.According to another aspect of the present invention, the present invention provides an anti-inflammatory cosmetic composition comprising, as an active ingredient, a fullerene-chungdae complex including fullerene, a blueberry extract, and a polymer.

본 발명에 따른 항염증용 화장료 조성물에 있어서, 풀러린-청대 복합체는 화장료 조성물 총 중량 대비 0.01 내지 80중량%, 바람직하게는 0.1 내지 50중량% 포함되는 것을 특징으로 한다. 풀러린-청대 복합체의 함량이 0.1중량% 미만일 경우 항염증 효과가 충분히 나타나지 않을 수 있고, 50중량% 초과할 경우 포함되는 풀러린-청대 복합체 함량 대비 효과가 현저하지 못하여 경제적인 측면에서 바람직하지 못하다.In the anti-inflammatory cosmetic composition according to the present invention, the fullerene-blue complex is characterized in that it is included in an amount of 0.01 to 80% by weight, preferably 0.1 to 50% by weight, based on the total weight of the cosmetic composition. When the content of the fullerene-blue complex is less than 0.1% by weight, the anti-inflammatory effect may not be sufficiently exhibited, and when it exceeds 50% by weight, the effect compared to the content of the fullerene-blue complex is not remarkable, which is not preferable from an economic point of view.

본 발명에 따른 항염증용 화장료 조성물에 있어서, 상기 화장료 조성물은 토너, 로션, 에센스, 세럼, 크림, 비누, 클렌징폼, 클렌징로션, 클렌징크림, 클렌징워터, 바디워시, 바디로션, 샴푸, 린스, 트리트먼트, 팩, 선크림 및 파운데이션으로 구성된 군으로부터 선택된 하나의 제형으로 제형화 되는 것을 특징으로 한다.In the anti-inflammatory cosmetic composition according to the present invention, the cosmetic composition is a toner, lotion, essence, serum, cream, soap, cleansing foam, cleansing lotion, cleansing cream, cleansing water, body wash, body lotion, shampoo, rinse, It is characterized in that it is formulated into one formulation selected from the group consisting of treatment, pack, sunscreen, and foundation.

본 발명의 다른 한 양태에 따르면, 본 발명은 풀러린, 청대 추출물 및 고분자를 포함하는 풀러린-청대 복합체를 유효성분으로 포함하는 항염증용 피부외용제 조성물을 제공한다.According to another aspect of the present invention, the present invention provides an anti-inflammatory composition for external application for skin comprising, as an active ingredient, a fullerene-chungdae complex including fullerene, a blueberry extract, and a polymer.

본 발명에 따른 항염증용 피부외용제 조성물에 있어서, 풀러린-청대 복합체는 피부외용제 조성물 총 중량 대비 0.01 내지 80중량%, 바람직하게는 0.1 내지 50중량% 포함되는 것을 특징으로 한다. 풀러린-청대 복합체의 함량이 0.1중량% 미만일 경우 항염증 효과가 충분히 나타나지 않을 수 있고, 50중량% 초과할 경우 포함되는 풀러린-청대 복합체 함량 대비 효과가 현저하지 못하여 경제적인 측면에서 바람직하지 못하다.In the anti-inflammatory composition for external application for skin according to the present invention, the fullerene-green complex is characterized in that it is included in an amount of 0.01 to 80% by weight, preferably 0.1 to 50% by weight, based on the total weight of the composition for external application for skin. When the content of the fullerene-blue complex is less than 0.1% by weight, the anti-inflammatory effect may not be sufficiently exhibited, and when it exceeds 50% by weight, the effect compared to the content of the fullerene-blue complex is not remarkable, which is not preferable from an economic point of view.

본 발명에 따른 항염증용 피부외용제 조성물에 있어서, 상기 피부외용제는 외용산제, 외용정제, 외용액제, 연고제, 크림제, 겔제, 경고제, 드레싱제, 패취제, 스프레이제 및 좌제로 구성된 군으로부터 선택된 하나의 제형으로 제형화 되는 것을 특징으로 한다.In the composition for external application for skin for anti-inflammatory use according to the present invention, the external application for skin is selected from the group consisting of powders for external use, tablets for external use, liquids for external use, ointments, creams, gels, warning agents, dressings, patches, sprays and suppositories. It is characterized in that it is formulated into one dosage form.

본 발명의 일 실험예에 따르면, 본 발명에 따른 풀러린-청대 복합체의 세포 독성을 확인한 결과, 대조군 대비 낮은 농도에서도 세포 독성이 없음을 확인하였다(실험예 3 참조). 또한, 본 발명에 따른 풀러린-청대 복합체의 항염증 효과를 확인한 결과, 대조군 대비 낮은 농도에서도 현저한 항염증 효과를 나타내는 것을 확인하였다(실험예 4 참조). 이러한 결과는 풀러린 및 청대 추출물이 고분자 매트릭스에 포집된 나노입자 형태의 신규한 소재인 풀러린-청대 복합체를 항염증용 화장료 조성물 또는 피부외용제 조성물의 유효성분으로 이용가능함을 시사한다.According to one experimental example of the present invention, as a result of confirming the cytotoxicity of the fullerene-cheongdae complex according to the present invention, it was confirmed that there was no cytotoxicity even at a lower concentration than the control group (see Experimental Example 3). In addition, as a result of confirming the anti-inflammatory effect of the fullerene-cheongdae complex according to the present invention, it was confirmed that a significant anti-inflammatory effect was exhibited even at a lower concentration than the control group (see Experimental Example 4). These results suggest that the fullerene-Cheondae complex, which is a novel material in the form of nanoparticles in which fullerene and Cheongdae extract are trapped in a polymer matrix, can be used as an active ingredient in an anti-inflammatory cosmetic composition or an external skin composition.

전술한 바와 같이, 본 발명에 풀러린-청대 복합체는 분산성이 우수하여 제품의 제형화에 용이할 뿐 아니라 제품의 안정성이 우수하며, 풀러린 및 청대에 의한 항염증 효과 또한 현저하여 항염증용 화장료 조성물 및 항염증용 피부외용제 조성물로서 이용 가능하다.As described above, the fullerene-green belt complex in the present invention has excellent dispersibility, so it is easy to formulate products, and the stability of the product is excellent, and the anti-inflammatory effect of fullerene and blue belt is also remarkable, so it is an anti-inflammatory cosmetic composition. and an anti-inflammatory composition for external application for skin.

도 1은 천연 풀러린의 산화도를 확인한 결과이다.
도 2는 본 발명의 풀러린-청대 복합체의 제조 공정도 이다.
도 3은 본 발명의 풀러린-청대 복합체의 특성을 확인한 결과이다.
도 4는 본 발명의 풀러린-청대 복합체의 세포 독성을 확인한 결과이다.
도 5는 본 발명의 풀러린-청대 복합체의 항염증 효과를 확인한 결과이다.1 is a result of confirming the degree of oxidation of natural fullerene.
2 is a manufacturing process diagram of the fullerene-green belt complex of the present invention.
3 is a result of confirming the characteristics of the fullerene-blue band complex of the present invention.
4 is a result of confirming the cytotoxicity of the fullerene-blue complex of the present invention.
5 is a result confirming the anti-inflammatory effect of the fullerene-blue complex of the present invention.

이하, 실시예를 통하여 본 발명을 더욱 상세히 설명하기로 한다. 이들 실시예는 단지 본 발명을 예시하기 위한 것이므로, 본 발명의 범위가 이들 실시예에 의해 제한되는 것으로 해석되지는 않는다.Hereinafter, the present invention will be described in more detail through examples. Since these examples are intended to illustrate the present invention only, the scope of the present invention is not to be construed as being limited by these examples.

준비예 1: 천연 풀러린의 제조Preparation Example 1: Preparation of natural fullerene

šœ가이트 1kg을 10um의 입도로 분쇄한 다음, šœ가이트에 톨루엔 40ml 및 에탄올 10ml를 혼합한 유기혼합용매를 첨가하여 30분 동안 교반기로 교반하고, 110℃에서 12시간 동안 열처리하였다. 그 후, 필터를 이용하여 여과한 šœ가이트 분말에 수산화나트륨 5kg, 정제수 20L를 가해 130℃에서 150시간 동안 추출하고, 이를 물로 7~10회 세척한 후 150℃로 건조하여 천연 풀러린을 수득하였다.1 kg of gate was pulverized to a particle size of 10um, and an organic mixed solvent of 40ml of toluene and 10ml of ethanol was added to the gate, stirred with a stirrer for 30 minutes, and heat-treated at 110°C for 12 hours. Thereafter, 5 kg of sodium hydroxide and 20 L of purified water were added to the gite powder filtered using a filter, and extraction was performed at 130 ° C for 150 hours, washed with water 7 to 10 times, and then dried at 150 ° C to obtain natural fullerene. .

준비예 2: 산화 풀러린의 제조Preparation Example 2: Preparation of oxidized fullerene

준비예 1에서 제조한 천연 풀러린을 증류수를 석영 큐벳에 준비한 후, UV 블랙라이트 램프(356nm)를 이용하여 24시간 동안 UV를 조사하여 산화 풀러린을 수득하였다.After preparing the natural fullerene prepared in Preparation Example 1 in a quartz cuvette with distilled water, UV was irradiated for 24 hours using a UV blacklight lamp (356 nm) to obtain oxidized fullerene.

준비예 3: 청대 추출물의 제조Preparation Example 3: Preparation of Cheongdae extract

건조된 청대를 준비한 후, 물을 건조된 청대 부피 대비 15 내지 25배로 첨가하여 80℃에서 2시간 동안 열수 추출하였다.After preparing the dried cheongdae, water was added in an amount of 15 to 25 times the volume of the dried cheongdae, followed by hot water extraction at 80° C. for 2 hours.

실험예 1: 산화 풀러린의 산화도 확인Experimental Example 1: Confirmation of degree of oxidation of oxidized fullerene

준비예 2에서 제조한 산화 풀러린의 산화 정도를 FT-IR을 이용하여 확인하였으며, 그 결과를 도 1에 나타내었다.The degree of oxidation of the oxidized fullerene prepared in Preparation Example 2 was confirmed using FT-IR, and the results are shown in FIG.

그 결과 도 1에서 확인할 수 있듯이, 천연 풀러린이 UV에 의해 24시간 산화되어 에테르기(C-O-C)의 산소 피크(806nm, 1000nm, 1018nm)를 확인 할 수 있으며, 이는 UV조사를 통해 산화 정도가 증가함을 알 수 있다. 이와 같이 풀러린의 산화에 의한 산소 피크의 증가는 풀러린의 물에서의 분산성을 증가시킬 수 있다.As a result, as can be seen in Figure 1, natural fullerene is oxidized by UV for 24 hours, and the oxygen peaks (806 nm, 1000 nm, 1018 nm) of the ether group (C-O-C) can be confirmed, which increases the degree of oxidation through UV irradiation. can know As such, the increase in the oxygen peak due to the oxidation of fullerene may increase the dispersibility of fullerene in water.

실시예: 풀러린-청대 복합체의 제조Example: Preparation of fullerene-blue complex

하이드록시에틸셀룰로오스(Hydroxyethylcellulose)와 상기 준비예 3에서 제조한 청대 추출물을 혼합하여 1wt%의 Hydroxyethylcellulose-청대추출물 용액을 준비하였다. 별도의 용기에 준비예 2에서 제조한 산화 풀러린과 준비예 3에서 제조한 청대 추출물을 혼합하여 1wt%의 천연 풀러린-청대 추출물 용액을 준비하였다. 상기에서 준비한 Hydroxyethylcellulose-청대추출물 용액과 천연 풀러린-청대 추출물 용액을 1:1로 혼합하여 1시간 동안 교반하였다. 교반 후 분산성과 나노사이즈화를 위해 초음파 분산기(sonicator)를 이용하여 2시간 동안 10s on, 5s off의 조건으로 초음파 분산하였다. 이 후 10분동안 3000rpm 20℃의 조건으로 원심분리한 다음 상등액을 제거를 통해 이물질 제거하여 풀러린-청대 복합체를 수득하였다.Hydroxyethylcellulose was mixed with the Cheongdae extract prepared in Preparation Example 3 to prepare a 1 wt% Hydroxyethylcellulose-Cheondae extract solution. A 1 wt% natural fullerene-Cheondae extract solution was prepared by mixing the oxidized fullerene prepared in Preparation Example 2 and the Cheongdae extract prepared in Preparation Example 3 in a separate container. The Hydroxyethylcellulose-Cheondae extract solution prepared above and the natural fullerene-Cheondae extract solution were mixed at a ratio of 1:1 and stirred for 1 hour. After stirring, ultrasonic dispersion was performed under conditions of 10 s on and 5 s off for 2 hours using a sonicator for dispersibility and nano-sizing. Thereafter, centrifugation was performed at 3000 rpm and 20° C. for 10 minutes, and then foreign substances were removed by removing the supernatant to obtain a fullerene-green complex.

실험예 2: 풀러린-청대 복합체의 특성 확인Experimental Example 2: Confirmation of characteristics of fullerene-blue complex

실시예에서 제조한 풀러린-청대 복합체의 입자크기와 분산도를 나노입도 및 제타전위 분석기를 이용하여 측정하였으며, 그 결과를 도 3에 나타내었다. The particle size and dispersion of the fullerene-blue complex prepared in Examples were measured using a nanoparticle size and zeta potential analyzer, and the results are shown in FIG. 3.

그 결과 도 3에서 확인할 수 있듯이, 입자크기와 분산도는 각각 약 360nm, 0.16 으로, 풀러린-청대 복합체가 균일한 크기로 제조되었으며, 물에서의 분산성도 우수한 것을 확인하였다.As a result, as can be seen in FIG. 3, the particle size and dispersity were about 360 nm and 0.16, respectively, and the fullerene-blue complex was prepared with a uniform size, and it was confirmed that the dispersibility in water was excellent.

실험예 3: 풀러린-청대 복합체의 세포 독성 확인Experimental Example 3: Confirmation of cytotoxicity of fullerene-blue complex

Raw 264.7 cell을 배양하여 본 발명에 따른 풀러린-청대 복합체와 LPS(대조군)를 세포에 24시간 처리한 후, MTT 용액(3-(4, 5-dimethyl thiazol-2-yl)-2, 5 diphenyl-2H- tetrazolium bromide)을 넣어 2시간 배양하였다. 배양액을 완벽히 제거한 뒤 0.5% SDS, 25mM 염화수소(HCL)이 함유된 90% 이소프로판올(isopropanol) 100 μL를 첨가해 10분간 교반 후 마이크로플레이트 리더(Microplate reader) 장비를 이용해 570nm에서 흡광도를 측정하여 세포독성을 평가하였으며, 그 결과를 도 4에 나타내었다.After culturing Raw 264.7 cells and treating the cells with the fullerene-Cheongdae complex and LPS (control) for 24 hours, MTT solution (3-(4, 5-dimethyl thiazol-2-yl)-2, 5 diphenyl -2H- tetrazolium bromide) and incubated for 2 hours. After completely removing the culture medium, 100 μL of 90% isopropanol containing 0.5% SDS and 25 mM hydrogen chloride (HCL) was added and stirred for 10 minutes. was evaluated, and the results are shown in FIG. 4.

그 결과 도 4에서 확인할 수 있듯이, 대조군의 생존율 100%를 기준으로 하였을 때, 본 발명에 따른 풀러린-청대 복합체를 12.5%를 처리하였을 때는 약 15%의 생존율을 보였으나 농도가 낮아짐에 따라 세포독성이 줄어 3.125 농도에서는 약 80%의 생존율을 보여 세포독성이 없음을 확인하였다.As a result, as can be seen in FIG. 4, when the survival rate of the control group was 100%, when 12.5% of the fullerene-blue complex according to the present invention was treated, the survival rate was about 15%, but as the concentration decreased, the cytotoxicity It was confirmed that there was no cytotoxicity by showing a survival rate of about 80% at 3.125 concentration.

실험예 4: 풀러린-청대 복합체의 항염증 효과 확인Experimental Example 4: Confirmation of anti-inflammatory effect of fullerene-blue complex

Raw 264.7 cell을 96 well에 분주하여 24시간 배양한 후 상층액을 석션(suction)하였다. 상기 세포에 풀러린-청대 복합체를 독성이 나타나지 않는 농도 범위에서 농도별로 처리하였으며, 대조군으로는 무혈청 배양액(serum free media)에 LPS를 처리하여 이용하였다. 24시간 이후 상층액을 취해 그리스 시약(Griess reagent)을 넣고 NO 생성량을 마이크로플레이트 리더(Microplate reader) 장비를 이용하여 550nm에서 흡광도를 측정하여 NO 생성 저해율(NO production inhibition)을 측정하였으며, 그 결과를 도 5에 나타내었다.Raw 264.7 cells were dispensed into 96 wells, cultured for 24 hours, and the supernatant was suctioned. The cells were treated with the fullerene-Cheondae complex at each concentration within a concentration range that did not show toxicity, and as a control, serum free media was treated with LPS. After 24 hours, the supernatant was taken, Griess reagent was added, and the NO production amount was measured by measuring absorbance at 550 nm using a microplate reader to measure NO production inhibition. shown in Figure 5.

그 결과 도 5에서 확인할 수 있듯이, LPS를 처리한 Raw 264.7 cell을 대조군으로 하여 비교하였을 때, 본 발명에 따른 풀러린-청대 복합체의 항산화능은 약 40%로 유의한 효과를 보였다.As a result, as can be seen in FIG. 5, when compared with Raw 264.7 cells treated with LPS as a control, the antioxidant capacity of the fullerene-green complex according to the present invention was about 40%, showing a significant effect.

Claims (10)

고분자 매트릭스에 풀러린 및 청대 추출물이 포집된 나노입자 형태인,
풀러린, 청대 추출물 및 고분자를 포함하는 풀러린-청대 복합체.
In the form of nanoparticles in which fullerene and Cheongdae extract are trapped in a polymer matrix,
A fullerene-green bamboo complex containing fullerene, a blue bamboo extract, and a polymer.
 삭제delete 제1항에 있어서,
상기 풀러린은 자외선으로 산화처리된 풀러린인 것인, 풀러린-청대 복합체.
According to claim 1,
The fullerene is a fullerene oxidized with ultraviolet light, fullerene-blue complex.
 제1항에 있어서,
상기 고분자는 폴리에틸렌글리콜(polyethyleneglycol; PEG), 폴리이소프로필아크릴아미드(poly N-isopropylacrylamide; PNIPAM), 폴리메틸아크릴레이트(polymethylacrylate; PMA), 폴리메틸메타아크릴레이트(polymethylmetacrylate;PMMA), 폴리아크릴아미드, 폴리아크릴릭에시드(polyacrylic acide; PAA), 폴리메타아크릴릭에시드(polymethacrylic acid; PMAA), 폴리비닐피롤리돈(polyvinylpyrrolidone; PVP), 폴리에틸렌옥사이드(PEO), 폴리비닐알코올(polyvinylalcohol; PVA), 셀룰로오스(Cellulose), 콜라겐(Collagen), 알지네이트(Alginate), 키토산(Chitosan), 및 이들의 조합으로 구성된 군으로부터 선택되는 하나 이상의 친수성 고분자, 또는 폴리에틸렌글리콜 (polyethyleneglycol; PEG), 폴리이소프로필아크릴아미드(poly Nisopropylacrylamide; PNIPAM), 폴리메틸아크릴레이트(polymethylacrylate; PMA), 폴리메틸메타아크릴레이트(polymethylmetacrylate; PMMA), 폴리아크릴아미드(polyacrylamide; PAM), 폴리아크릴릭에시드(polyacrylic acide; PAA), 폴리메타아크릴릭에시드(polymethacrylic acid; PMAA), 폴리비닐피롤리돈(poly vinylpyrrolidone; PVP), 폴리비닐알코올(polyvinylalcohol; PVA), 셀룰로오스(Cellulose), 폴리에틸렌옥사이드(PEO), 콜라겐(Collagen), 알지네이트(Alginate), 키토산(Chitosan) 및 이들의 조합으로 이루어진 군에서 선택되는 것을 포함하는 선형, 블록, 그래프트 또는 덴드리머 친수성 고분자 블록, 및 이들의 조합으로 구성된 군으로부터 선택되는 하나 이상의 양친매성 고분자인 것인, 풀러린-청대 복합체.
According to claim 1,
The polymer is polyethyleneglycol (PEG), polyisopropylacrylamide (PNIPAM), polymethylacrylate (PMA), polymethylmethacrylate (PMMA), polyacrylamide, Polyacrylic acid (PAA), polymethacrylic acid (PMAA), polyvinylpyrrolidone (PVP), polyethylene oxide (PEO), polyvinylalcohol (PVA), cellulose ), at least one hydrophilic polymer selected from the group consisting of collagen, alginate, chitosan, and combinations thereof, or polyethylene glycol (PEG), poly isopropylacrylamide (poly Nisopropylacrylamide; PNIPAM), polymethylacrylate (PMA), polymethylmethacrylate (PMMA), polyacrylamide (PAM), polyacrylic acide (PAA), polymethacrylic acid PMAA), polyvinylpyrrolidone (PVP), polyvinylalcohol (PVA), cellulose, polyethylene oxide (PEO), collagen, alginate, chitosan And a linear, block, graft or dendrimer hydrophilic polymer block including one selected from the group consisting of combinations thereof, and one or more amphiphilic polymers selected from the group consisting of combinations thereof, fullerene-green belt complex.
 고분자와 청대 추출물을 혼합하여 제1 용액을 준비하는 제1 단계;
천연풀러린에 자외선을 조사하여 산화시켜 산화 풀러린을 수득하는 제2 단계;
상기 제2 단계에서 제조된 산화 풀러린과 청대 추출물을 혼합하여 제2 용액을 준비하는 제3 단계;
상기 제1 용액 및 제2 용액을 혼합한 혼합물을 교반 및 초음파 처리하는 제4 단계; 및
상기 제4 단계의 혼합물을 원심분리한 후 상등액을 제거하는 단계;를 포함하는,
고분자 매트릭스에 풀러린 및 청대 추출물이 포집된 나노입자 형태를 갖는 풀러린-청대 복합체의 제조방법.
A first step of preparing a first solution by mixing a polymer and a Cheongdae extract;
A second step of oxidizing natural fullerene by irradiating it with ultraviolet rays to obtain oxidized fullerene;
A third step of preparing a second solution by mixing the oxidized fullerene prepared in the second step with the Cheongdae extract;
A fourth step of stirring and sonicating a mixture of the first solution and the second solution; and
Including, centrifuging the mixture of the fourth step and removing the supernatant;
A method for preparing a fullerene-Cheondae complex in the form of nanoparticles in which fullerene and Cheongdae extract are trapped in a polymer matrix.
 제1항에 따른 풀러린-청대 복합체를 유효성분으로 포함하는 항염증용 화장료 조성물.
An anti-inflammatory cosmetic composition comprising the fullerene-green complex according to claim 1 as an active ingredient.
 제6항에 있어서,
상기 풀러린-청대 복합체는 화장료 조성물 총 중량 대비 0.1 내지 50중량% 포함되는 것인, 항염증용 화장료 조성물.
According to claim 6,
The fullerene-blue complex is contained in an amount of 0.1 to 50% by weight based on the total weight of the cosmetic composition, anti-inflammatory cosmetic composition.
 제6항에 있어서,
상기 화장료 조성물은 토너, 로션, 에센스, 세럼, 크림, 비누, 클렌징폼, 클렌징로션, 클렌징크림, 클렌징워터, 바디워시, 바디로션, 샴푸, 린스, 트리트먼트, 팩, 선크림 및 파운데이션으로 구성된 군으로부터 선택된 하나의 제형으로 제형화 되는 것인, 항염증용 화장료 조성물.
According to claim 6,
The cosmetic composition is from the group consisting of toner, lotion, essence, serum, cream, soap, cleansing foam, cleansing lotion, cleansing cream, cleansing water, body wash, body lotion, shampoo, rinse, treatment, pack, sunscreen and foundation Which is formulated in one selected formulation, a cosmetic composition for anti-inflammatory.
 제1항에 따른 풀러린-청대 복합체를 유효성분으로 포함하는 항염증용 피부외용제 조성물.
An anti-inflammatory composition for external application for skin comprising the fullerene-green complex according to claim 1 as an active ingredient.
 제9항에 있어서,
상기 피부외용제는 외용산제, 외용정제, 외용액제, 연고제, 크림제, 겔제, 경고제, 드레싱제, 패취제, 스프레이제 및 좌제로 구성된 군으로부터 선택된 하나의 제형으로 제형화 되는 것인, 항염증용 피부외용제 조성물.According to claim 9,
The skin external preparation is formulated into one formulation selected from the group consisting of external powders, external tablets, external solutions, ointments, creams, gels, warning agents, dressings, patches, sprays and suppositories, for anti-inflammatory use Composition for external application for skin.
KR1020210053168A 2021-04-23 2021-04-23 Fullerene-indigo pulverata levis complex, a preparation method thereof and a use of the same KR102548473B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1020210053168A KR102548473B1 (en) 2021-04-23 2021-04-23 Fullerene-indigo pulverata levis complex, a preparation method thereof and a use of the same

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1020210053168A KR102548473B1 (en) 2021-04-23 2021-04-23 Fullerene-indigo pulverata levis complex, a preparation method thereof and a use of the same

Publications (2)

Publication Number Publication Date
KR20220146198A KR20220146198A (en) 2022-11-01
KR102548473B1 true KR102548473B1 (en) 2023-06-28

Family

ID=84042283

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020210053168A KR102548473B1 (en) 2021-04-23 2021-04-23 Fullerene-indigo pulverata levis complex, a preparation method thereof and a use of the same

Country Status (1)

Country Link
KR (1) KR102548473B1 (en)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005281253A (en) * 2004-03-30 2005-10-13 Nof Corp Skin cosmetic

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE529094T1 (en) * 2003-01-27 2011-11-15 Vitamin C60 Biores Corp COMPOSITION FOR EXTERNAL USE
KR101633302B1 (en) 2013-11-01 2016-06-24 송남강 Cosmetic composition containing natural fullerene and method for manufacturing the same

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005281253A (en) * 2004-03-30 2005-10-13 Nof Corp Skin cosmetic

Also Published As

Publication number Publication date
KR20220146198A (en) 2022-11-01

Similar Documents

Publication Publication Date Title
KR102068173B1 (en) Mutifunctional bionanocomposite hydrogel, method of manufacturing thereof and use thereof
US20080226740A1 (en) Marine algal extracts comprising marine algal polysaccharides of low degree polymerizaton, and the preparation processes and uses thereof
KR20120139229A (en) Cosmetic composition for preventing skin aging comprising surface-modified gold nanoparticle by phytochemical
Nosrati et al. Cerium oxide nanoparticles: Synthesis methods and applications in wound healing
KR101144493B1 (en) Antibiotic cosmetic composition comprising alginate, fucoidan or silver-alginate having low molecular weight
Pagano et al. Development of sodium carboxymethyl cellulose based polymeric microparticles for in situ hydrogel wound dressing formation
KR20180119975A (en) Functionalized transition metal dichalcogenides and composition for antioxidation containing thereof
WO2023173671A1 (en) Oil-control composite material and preparation method therefor, and cosmetic
Manne et al. Pterocarpus marsupium Roxb. heartwood extract synthesized chitosan nanoparticles and its biomedical applications
KR100847478B1 (en) Composition for functional soap comprising gold or silver nanoparticle and extraction polymer of black nightshade as effective ingredients, and production method thereof
KR102548473B1 (en) Fullerene-indigo pulverata levis complex, a preparation method thereof and a use of the same
Di Marzio et al. Deformable surfactant vesicles loading ammonium glycyrrhizinate: characterization and in vitro permeation studies
CN116209426A (en) Anti-aging and antioxidant cosmetic composition comprising broccoli exosomes as active ingredient and functional cosmetic comprising the same
EP3936575A1 (en) Surface-treated inorganic particles, manufacturing method of the same, dispersion solution of the same, and cosmetic composition including the same
KR100551989B1 (en) Self-assembled polymeric nanoparticles containing ginsenosides and cosmetic compositions containing the nanoparticles
KR101054731B1 (en) Nanoparticles comprising finasteride, a poorly soluble hair growth and hair growth bioactive substance, and an external composition for skin containing the same
KR100967113B1 (en) Self-assembled polymeric nanoparticles containing coenzyme Q10 and skin-care compositions for exteral application, containing the nanoparticles
CN108938486B (en) Anticorrosive composite and its preparation method and application
KR101605517B1 (en) Manufacturing method for cosmetic composition for improving acne and cosmetic compostion thereof
KR101467162B1 (en) Essential Composition with Carboxy Generating Substance for Improving Blood Flow and Treating Acne and Method for Producing the Same
KR102349033B1 (en) Method for improving water-dispersibility of natural fullerene and use of natural fullerene prepared thereby
KR100876914B1 (en) Compostion for Fuctional Soap Comprising Silver Nanoparticle and Organic Germanium, and Production Method Thereof
KR102558182B1 (en) Moisturizing composition containing metal aminoclay nanoparticles and Opuntia humifusa extract
KR102316264B1 (en) Method for improving water-dispersibility of natural fullerene with amphiphilic polymers and use of natural fullerene prepared thereby
Ajmal et al. Fabrication of Silicon Dioxide (SiO2) Nanoparticles Using Wastes of Fruit’s Peel: Characterization and Biological Activities

Legal Events

Date Code Title Description
E902 Notification of reason for refusal
E701 Decision to grant or registration of patent right