KR102398406B1 - Novel deep sea water equisetum hyemale extract and the preparation method of the same - Google Patents
Novel deep sea water equisetum hyemale extract and the preparation method of the same Download PDFInfo
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Abstract
본 출원+은 항산화, 항주름, 항염 및 항균 기능이 증진된 신규한 해양심층수 속새 추출물의 제조방법 및 그 추출물에 관한 것이다.The present application + relates to a method for producing a novel deep-sea water prickly pear extract with enhanced antioxidant, anti-wrinkle, anti-inflammatory and antibacterial functions, and an extract thereof.
Description
본 출원은 항산화, 항주름, 항염 및 항균 기능이 증진된 신규한 해양심층수 속새 추출물 및 그 제조방법에 관한 것이다.[0001] The present application relates to a novel deep-sea water prickly pear extract with enhanced antioxidant, anti-wrinkle, anti-inflammatory and antibacterial functions, and a method for preparing the same.
속새 (Equisetum hyemale)는 속새과의 상록성 양치식물로, 관다발을 가지고 있으나, 꽃은 피우지 않는다. 줄기는 비어있고, 마디가 나있고, 전체가 녹색으로 엽록체가 있어, 광합성을 할 수 있다. 모양이 작은 대나무 같은 형태이고, 속초시 지명의 유래와 관련이 있는 식물로, 울산바위 전설과 연계되여, 뷰티소재로서의 스토리가 있는 식물이다. 본 기술에서는 팽화 기술의 적용을 통하여, 추출율과 생리기능성을 높이고, 또 해양심층수를 활용하여, 추출물의 용해도와 물성을 개선하고, 해양심층수의 기능성과의 시너지 효과를 가지는 조성물을 제공하고자 한다.Equisetum hyemale (Equisetum hyemale) is an evergreen fern in the family Equisetum, which has vascular bundles but does not produce flowers. Stems are hollow, segmented, green and have chloroplasts, allowing photosynthesis. It has a small bamboo-like shape and is related to the origin of the place name of Sokcho. It is a plant with a story as a beauty material in connection with the legend of Ulsanbawi Rock. In this technology, it is intended to provide a composition having a synergistic effect with the functionality of the deep sea water, improving the solubility and physical properties of the extract by increasing the extraction rate and physiological functionality through the application of the swelling technology, and utilizing deep sea water.
기존 기술은, 속새의 팽화기술에 의해 항산화도와 추출율을 높일 수 있었으나, 이때 전분을 첨가하여 일반 팽화 가공처리의 경우에는 해양심층수 원수에 의한 추출율이 오히려 떨어지는 문제점이 있었다. 따라서 본 발명에서는 전분을 첨가하는 속새의 팽화공정 대신에 전분을 첨가하지 않고, 속새를 해양심층수를 뿌려 팝핑한 후 일정량의 에탄올을 첨가한 해양심층수로 가열환류 추출하는 신규한 속새 추출 방법에 관한 것으로 이를 통하여, 팽화 팝핑에 의한 항산화도 증가효과와 해양심층수원수(DW)에 의한 추출율 증가 효과를 기대할수 있었다. 따라서 팽화 팝핑공정에서, 약간의 해양심층수 원수를 흩뿌려 준 다음 팽화한 후, 해양심층수 원수에의한 추출효과를 증진시킬수 있으며, 여기에 다시 해양심층수에 &0% 농도의 주정1 ∼ 50% 를 추가로 더 첨가하여 가열 환류 추출함으로써 항산화도와 기능성이 증진된 신규한 해양심층수 속새 추출물을 제조할 수 있음을 확인하였다.In the existing technology, the degree of antioxidant and extraction rate could be increased by the swelling technology of sagebrush, but in the case of general swelling processing by adding starch at this time, there was a problem that the extraction rate by deep sea water raw water was rather decreased. Therefore, in the present invention, instead of adding starch, instead of adding starch, instead of adding starch, deep-sea water is sprayed and popped, followed by heating and reflux extraction with deep-sea water to which a certain amount of ethanol has been added. Through this, it was possible to expect the effect of increasing the antioxidant level by the swelling popping and increasing the extraction rate by the deep sea water (DW). Therefore, in the expansion popping process, after sprinkling some deep sea water raw water and swelling it, the extraction effect by the deep sea water raw water can be enhanced. It was confirmed that a novel deep-sea water extract with improved antioxidant and functional properties could be prepared by heating and reflux extraction by further adding
종래 공지된 선행기술문헌으로는 대한민국 등록특허 제 1017750730000 가 있으나, 이는 속새 추출물과 식물 발효액을 혼합하여 발효시킨 식물 혼합 속새 발효액을 유효성분으로 하는 항산화용 조성물 및 그의 제조방법에 관한 것이며, 또 대한민국 등록특허 제 1016125010000 가 있으나 이는 속새, 산약, 토사자 및 선모 추출물을 유효성분으로 포함하는 남성의 생식기능 개선용 조성물에 관한 것이다. 따라서 상기 문헌 어디에도 피부 항산화, 항주름, 항염 및 항균 기능이 뛰어난 해양심층수 속새 추출물과 그 제조방법에 대하여는 암시되거나 개시된 연구는 전무하다.As a prior art document known in the prior art, there is Korean Patent Registration No. 1017750730000, which relates to an antioxidant composition using, as an active ingredient, a plant mixed fermented fermented fermented plant fermented fermented plant extract and plant fermented broth, and a method for preparing the same, and is also registered in the Republic of Korea. Although there is a patent No. 1016125010000, it relates to a composition for improving male reproductive function, which includes an extract of Soksaae, Sanyak, Tosaja and Peony hair as active ingredients. Therefore, there are no studies suggested or disclosed on the deep sea water serrata extract and its preparation method, which are excellent in skin antioxidant, anti-wrinkle, anti-inflammatory and antibacterial functions, nowhere in the literature.
이러한 배경 하에, 본 발명자들은 신규한 해양심층수 속새 추출물을 개발하기 위하여 예의 연구 노력한 결과 피부 항산화 및 다기능성을 갖는 보관성이 증진된 특성이 있는 해양심층수 속새 추출물을 발명함으로써 본 출원을 완성하였다.Under this background, the present inventors completed the present application by inventing an extract of deep-sea water serrata having improved storability with skin antioxidant and multifunctional properties as a result of intensive research efforts to develop a novel deep-sea serrata extract.
삭제delete
본 출원의 목적은 지금까지 공지된 것과 다른 신규한 해양심층수를 이용한 속새 추출물의 제조방법을 제공하는 것이다.It is an object of the present application to provide a method for preparing an extract of serrata by using a novel deep sea water different from that known so far.
본 출원의 다른 목적은 상기 방법으로 제조된 기능성이 재고된 신규한 해양심층수 속새 추출물을 제공하는 것이다.Another object of the present application is to provide a novel deep-sea water prickly pear extract with reconsidered functionality prepared by the above method.
본 출원의 다른 목적은 상기 신규한 해양심층수 속새 추출물을 유효성분으로 함유하는 항산화 등 다기능성 화장료 조성물 및 그 화장료를 제공하는 것이다.Another object of the present application is to provide a multifunctional cosmetic composition such as an antioxidant and a cosmetic comprising the novel deep-sea water serrata extract as an active ingredient.
본 출원의 다른 목적 및 이점은 첨부한 청구범위 및 도면과 함께 하기의 상세한 설명에 의해 보다 명확해질 것이다. 본 명세서에 기재되지 않은 내용은 본 출원의 기술 분야 또는 유사 분야에서 숙련된 자이면 충분히 인식하고 유추할 수 있는 것이므로 그 설명을 생략한다.Other objects and advantages of the present application will become more apparent from the following detailed description in conjunction with the appended claims and drawings. Content not described in this specification will be omitted because it can be sufficiently recognized and inferred by those skilled in the technical field or similar field of the present application.
이하, 본 출원 내용에 대하여 구체적으로 설명하면 다음과 같다. 본 출원의 상기 목적은 건조된 속새 시료에 해양심층수를 뿌려 팽화기에 내부온도 180℃ 에서 7분간 팝핑된 속새 팽화물을 수득하고, 상기 수득된 속새 팽화물에 농도 70% 의 주정 1 ∼ 50%(w/w)를 추가 더 첨가한 해양심층수로 가열 환류 추출하는 것을 포함하여 이루어진다. 한편, 본 출원에서 개시한 일 실시 양태의 설명 및 실시 형태는 공통된 사항에 대하여 다른 양태의 설명 및 실시 형태에도 적용될 수 있다. 또한, 본 출원에서 개시된 다양한 요소들의 모든 조합이 본 출원의 범주에 속한다. 더불어, 하기 기술된 구체적인 서술에 의하여 본 출원의 범주가 제한된다고 볼 수 없다.Hereinafter, the content of the present application will be described in detail as follows. The above object of the present application is to spray the dried seaweed sample with deep-sea water to obtain expanded horseradish popped at an internal temperature of 180° C. for 7 minutes in an expander, and 1 to 50% of alcohol at a concentration of 70% ( w / w) is made including heating and reflux extraction with additionally added deep sea water. On the other hand, descriptions and embodiments of one embodiment disclosed in the present application may be applied to descriptions and embodiments of other aspects with respect to common matters. Also, all combinations of the various elements disclosed in this application are within the scope of this application. In addition, it cannot be seen that the scope of the present application is limited by the detailed description described below.
본 출원의 목적을 달성하기 위하여 일 양태로서 신규한 해양심층수 속새 추출물의 제조방법을 제공한다. In order to achieve the object of the present application, as an aspect, there is provided a method for preparing a novel deep-sea water prickly pear extract.
본 출원은 다른 양태로서 신규한 다기능성 해양심층수 속새 추출물을 제공한다.As another aspect, the present application provides a novel multifunctional deep-sea water prickly pear extract.
본 출원은 다른 양태로서 해양심층수 속새 추출물을 유효성분으로 함유하는 항산화 및 다기능성 화장료 조성물을 제공한다As another aspect, the present application provides an antioxidant and multifunctional cosmetic composition containing an extract of deep-sea water serrata as an active ingredient.
본 출원은 다른 양태로서 상기 화장료 조성물이 화장수, 로션, 크림 및 에센스 중에서 선택되는 어느 하나의 제형인 것이 특징인 항산화 및 다기능성 화장료를 제공한다. As another aspect, the present application provides an antioxidant and multifunctional cosmetic, wherein the cosmetic composition is a formulation selected from a lotion, a lotion, a cream, and an essence.
상기 화장료 조성물은 화장수, 로션, 용액, 현탁액, 유탁액, 페이스트, 겔, 크림, 로션, 에센스, 샴푸, 계면활성제 함유 클렌징, 토닉, 스케일링 등으로 제형화 될 수 있으나, 이에 한정되는 것은 아니다. 보다 구체적으로는, 유연 화장수, 영양 화장수, 영양 유액, 영양 크림, 마사지 크림, 에센스, 아이크림, 클렌징크림, 클렌징 폼, 클렌징워터, 팩, 헤어 에센스, 린스 등의 제형으로 제조될 수 있다. The cosmetic composition may be formulated as a lotion, lotion, solution, suspension, emulsion, paste, gel, cream, lotion, essence, shampoo, surfactant-containing cleansing, tonic, scaling, etc., but is not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, a nourishing lotion, a nourishing emulsion, a nourishing cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a hair essence, a rinse, and the like.
본 출원은 다기능성 신규한 해양심층수 속새 추출물과 그 제조방법을 제공하는 효과가 있고, 상기 다기능성 속새 추출물은 항산화 및 항주름, 항염, 항균 등 다기능성 효능이 뛰어난 항산화 및 다기능성 화장료 조성물로 이용될 수 있는 뛰어난 효과가 있다.The present application has the effect of providing a novel multifunctional deep-sea water serrata extract and a method for producing the same, and the multi-functional locust extract is used as an antioxidant and multi-functional cosmetic composition with excellent multi-functional effects such as antioxidant, anti-wrinkle, anti-inflammatory, and antibacterial. There is a great effect that can be.
도 1a는 본 발명에 따른 팝핑 전 속새의 사진도(SS-b)이다.
도 1b는 본 발명에 따른 팝핑 후 속새의 사진도(SS-a)이다.
도 2는 본 발명에 따른 팝핑 속새의 냉침 추출물의 사진도이다.
도 3a는 본 발명에 따른 속새 추출물의 항산화 기능을 나타내는 그래프이다. (SS-b, 팝핑 전; SS-a, 팝핑 후)
도 3b는 본 발명에 따른 속새 추출물의 비타민 측정을 나타내는 그래프이다.
도 4는 본 발명에 따른 팝핑 전 속새 추출물의 HPLC 크로마토그램을 나타내는 그래프이다.
도 5는 본 발명에 따른 팝핑 후 속새 추출물의 HPLC 크로마토그램을 나타내는 그래프이다.
도 6a는 본 발명에 따른 팽화 전 전분을 첨가한 속새 팽화물의 사진도이다.
도 6b는 본 발명에 따른 팽화 후 전분을 첨가한 속새 팽화물의 사진도이다.
도 7a는 본 발명에 따른 심층수원수로 추출 후 전분을 첨가하여 팽화한 속새 추출물의 사진도이다.(좌/ 팽화 전; 우/팽화 후)
도 7b는 본 발명에 따른 심층수농축수로 추출 후 전분을 첨가하여 팽화한 속새 추출물의 사진도이다.(좌/ 팽화 전; 우/팽화 후)
도 7c는 본 발명에 따른 증류수로 추출 후 전분을 첨가하여 팽화한 속새 추출물의 사진도이다.(좌/ 팽화 전; 우/팽화 후)
도 7d는 본 발명에 따른 에탄올로 추출 후 전분을 첨가하여 팽화한 속새 추출물의 사진도이다.(좌/ 팽화 전; 우/팽화 후)
도 8a는 본 발명에 따른 심층수원수로 추출 후 전분을 첨가하여 팽화한 속새 추출물의 ABTS 라디컬 소거능을 측정한 사진도이다.
도 8b는 본 발명에 따른 심층수농축수로 추출 후 전분을 첨가하여 팽화한 속새 추출물의 ABTS 라디컬 소거능을 측정한 사진도이다.
도 8c는 본 발명에 따른 증류수로 추출 후 전분을 첨가하여 팽화한 속새 추출물의 ABTS 라디컬 소거능을 측정한 사진도이다.
도 8d는 본 발명에 따른 에탄올로 추출 후 전분을 첨가하여 팽화한 속새 추출물의 ABTS 라디컬 소거능을 측정한 사진도이다.
도 9a는 본 발명에 따른 심층수원수로 추출 후 전분을 첨가하여 팽화한 속새 추출물의 ABTS 라디컬 소거능을 측정한 그래프이다.
도 9b는 본 발명에 따른 심층수농축수로 추출 후 전분을 첨가하여 팽화한 속새 추출물의 ABTS 라디컬 소거능을 측정한 그래프이다.
도 9c는 본 발명에 따른 증류수로 추출 후 전분을 첨가하여 팽화한 속새 추출물의 ABTS 라디컬 소거능을 측정한 그래프이다.
도 9d는 본 발명에 따른 에탄올로 추출 후 전분을 첨가하여 팽화한 속새 추출물의 ABTS 라디컬 소거능을 측정한 그래프이다.Figure 1a is a photograph (SS-b) of the popping before popping according to the present invention.
Figure 1b is a photographic view (SS-a) of the prickly pear after popping according to the present invention.
2 is a photographic diagram of the extract of the cold-chim from popping sagebrush according to the present invention.
Figure 3a is a graph showing the antioxidant function of the extract according to the present invention. (SS-b, before popping; SS-a, after popping)
Figure 3b is a graph showing the vitamin measurement of the extract according to the present invention.
Figure 4 is a graph showing the HPLC chromatogram of the extract before popping according to the present invention.
Figure 5 is a graph showing the HPLC chromatogram of the extract after popping according to the present invention.
Figure 6a is a photographic view of the expanded product of the present invention to which starch was added before expansion.
Figure 6b is a photographic view of the expanded product of the present invention to which starch is added after expansion.
Figure 7a is a photograph of an extract of serrata by adding starch after extraction with deep water source according to the present invention. (Left/Before swelling; Right/After swelling)
Figure 7b is a photograph of the extract of serrata by adding starch after extraction with deep water concentrated water according to the present invention. (Left/Before swelling; Right/After swelling)
Figure 7c is a photograph of an extract of serrata by adding starch after extraction with distilled water according to the present invention. (Left/Before swelling; Right/After swelling)
7d is a photograph of an extract of serrata after extracting with ethanol according to the present invention and then swelling by adding starch. (Left/Before swelling; Right/After swelling)
FIG. 8a is a photograph showing the ABTS radical scavenging ability of an extract of serrata swelled by adding starch after extraction with deep water source according to the present invention.
FIG. 8b is a photograph showing the ABTS radical scavenging ability of the serrata extract expanded by adding starch after extraction with deep water concentrated water according to the present invention.
FIG. 8c is a photograph showing the ABTS radical scavenging ability of an extract of serrata blotted by adding starch after extraction with distilled water according to the present invention.
FIG. 8d is a photograph showing the ABTS radical scavenging ability of an extract of S. serrata, which was expanded by adding starch after extraction with ethanol according to the present invention.
Figure 9a is a graph measuring the ABTS radical scavenging ability of the extract expanded by adding starch after extraction with deep water source according to the present invention.
Figure 9b is a graph measuring the ABTS radical scavenging ability of the extract expanded by adding starch after extraction with deep water concentrated water according to the present invention.
FIG. 9c is a graph measuring the ABTS radical scavenging ability of an extract of serrata, which was expanded by adding starch after extraction with distilled water according to the present invention.
FIG. 9d is a graph measuring the ABTS radical scavenging ability of an extract of serrata blotted with starch after extraction with ethanol according to the present invention.
이하, 본 출원에 따른 실시예를 통하여 본 발명을 보다 상세히 설명하고자 한다. 다만 본 출원의 하기 실시예는 본 출원의 일 예시에 불과하다. 이들 실시예는 본 발명을 보다 구체적으로 설명하기 위한 것으로서, 첨부된 청구항에 제시된 본 발명의 범위가 이들 실시예에 의해 제한되지 않는다는 것은 본 발명이 속하는 기술분야에서 통상의 지식을 가진 자에게 있어 명백할 것이다.Hereinafter, the present invention will be described in more detail through examples according to the present application. However, the following examples of the present application are merely examples of the present application. These examples are for explaining the present invention in more detail, and it is clear to those of ordinary skill in the art that the scope of the present invention presented in the appended claims is not limited by these examples. something to do.
실시예 1. 속새 팝핑물의 제조방법Example 1. Manufacturing method of popping material
절단되고 건조된 속새를 준비한 후 전기식 재래식 뻥튀기 기계를 70℃로 세팅으로 예열(기계 내부온도 약 280℃)한 후, 50℃ 세팅을 하고 내부를 식혔다. 이때 기계 내부온도는 가장 바람직하기는 180℃ 이었다. 다음, 속새를 넣고 가장 바람직하게는 7분간 다시 가열한 후, 순간적으로 뚜껑을 열고 시료를 급속 팝핑하여 본 발명 속새 팝핑물(이하, 팽화물이라 함)을 제조하였고 팝핑 후 속새와 팝핑 전 속새는 각각 도 1a와 도 1b에 나타내었다.After preparing the cut and dried scallops, preheat the electric conventional frying machine at 70°C (the machine internal temperature is about 280°C), then set it to 50°C and cool the inside. At this time, the internal temperature of the machine was most preferably 180°C. Next, after putting the sagebrush and most preferably after heating again for 7 minutes, the cap is opened instantaneously and the sample is rapidly popped to prepare the present invention poppy (hereinafter referred to as the expanded product). 1A and 1B, respectively.
실시예 2. 전분을 첨가한 속새 팽화물의 제조방법Example 2. Method for preparing the expanded product of starch added
팽화기를 110℃로 세팅 한 후 30분간 예열한 후, 밀가루 100g을 물과 반죽하여 시료를 넣기 전 투입하여 사출구로 나올 때까지 확인한 다음, 그 후 소량의 현미가루를 투입하여 팽화가 되는지 확인하였다. 다음, 현미가루 900g과 절단된 속새100g을 섞어 팽화기에 투입하고 동일하게 설정된 조건에서 팽화시켜 전분 물질이 첨가된 속새 팽화물을 얻었다.After setting the expander at 110 ° C. and preheating for 30 minutes, knead 100 g of wheat flour with water, put it in before adding the sample, and check it until it comes out of the ejection port, and then add a small amount of brown rice powder to check whether swelling occurs. Next, 900 g of brown rice flour and 100 g of cut soybean paste were mixed, put into a expander, and expanded under the same set conditions to obtain a starch material-added expanded soybean paste.
실시예 3. 해양심층수 속새 팝핑물의 제조방법Example 3. Method for producing deep-sea water prickly pear popping water
상기 실시예 1과 달리 속새 팽화물을 제조하기 위하여 팝핑시 해양심층수 원수와 농축수를 각각 일정량의 주정과 함께 각각 뿌려주고 추출율 및 특성을 확인하였다.Unlike Example 1 above, in order to prepare a safflower, raw water and concentrated water were respectively sprayed with a certain amount of alcohol during popping, and the extraction rate and characteristics were checked.
실험예 1. 팝핑한 시료의 성분 분석 및 항산화도 분석Experimental Example 1. Component analysis and antioxidant level analysis of popped samples
우선 팝핑하지 않은 시료와 팝핑한 시료 2g 또는 3g을 각각 넣고 20배의 70% 에탄올을 용매로 하여 17시간 동안 냉침 추출(도 2)한 다음 whatman No.1 filter paper로 여과하여 에탄올 추출물의 여액을 수득하였다. First, add 2g or 3g of unpopped and popped samples, respectively, and extract with 20
상기 속새 추출물의 여액의 성분분석은 HPLC와 ABTS 라디칼 소거능을 동시에 측정할 수 있는 online HPLC-ABTS를 이용하였다. 사용한 Column은 YMC-Triart C18을 이용하였고 이동상은 0.1% formic acid가 들어간 Acetonitrile과 Water를 이용하였으며, ABTS 시약은 3mM ABTS와 2.4mM potassium persulfate를 넣고 암소에서 16시간 동안 반응한 후 사용하였다. 추출물의 여액은 Rotary evaporator를 이용하여 농축 한 후 성분분석을 진행하였다. HPLC의 반응 조건은 다음의 표 1과 같다.For the component analysis of the filtrate of the extract, HPLC and online HPLC-ABTS, which can simultaneously measure the ABTS radical scavenging ability, were used. The column used was YMC-Triart C18, the mobile phase was acetonitrile containing 0.1% formic acid and water, and the ABTS reagent was 3mM ABTS and 2.4mM potassium persulfate, followed by reaction in a dark place for 16 hours. The filtrate of the extract was concentrated using a rotary evaporator, and then component analysis was performed. The reaction conditions of HPLC are shown in Table 1 below.
상기 추출물 여액의 농축물의 항산화도 분석은 DPPH 라디칼 소거능 측정법 (Blois MS., 1958, Nature, 181:1199-1200)을 이용하였다. 즉, 일정농도의 시료에 0.2mM DPPH 용액을 넣고 혼합 한 후 암소에서 30분간 반응 후 517nm에서 흡광도 측정하였다. DPPH 라디칼 소거능(DPPH radical scavenging activity, %)은 하기 식으로 계산하였으며, 3회 반복 실험하여 얻은 결과를 평균과 표준편차로 나타내었다.Analysis of the antioxidant level of the concentrate of the extract filtrate was performed using a DPPH radical scavenging method (Blois MS., 1958, Nature, 181:1199-1200). That is, 0.2 mM DPPH solution was added to a sample of a certain concentration, mixed, and then reacted in a dark place for 30 minutes, and absorbance was measured at 517 nm. DPPH radical scavenging activity (%) was calculated by the following formula, and the results obtained by repeating the experiment three times are expressed as the average and standard deviation.
상기 방법에 따라 팝핑 전(SS-b)과 팝핑 후(SS-a)의 속새 추출물의 항산화도가 측정되었고 그 결과는 도 3a와 도 3b, 표 2에 나타나 있다. 또한, 표 3에 비타민 C 의 측정결과가 기재되어 있다.According to the above method, the antioxidant levels of the extract before (SS-b) and after (SS-a) popping were measured, and the results are shown in FIGS. 3A and 3B and Table 2. In addition, Table 3 describes the measurement results of vitamin C.
또한, 팝핑 전과 후의 속새 추출물의 HPLC 크로마토그램은 도 4 및 도 5a 및 도 5b에 나타나 있다.In addition, HPLC chromatograms of the extracts of Sokki before and after popping are shown in FIGS. 4 and 5A and 5B .
실험예 2. 전분을 첨가하여 팽화한 속새추출물의 항산화도 분석Experimental Example 2. Analysis of the antioxidant level of serrata extract expanded by adding starch
전분을 첨가한 속새 팽화물은 도 6 및 도 7 에 나타나 있다.The cornstarch expanded product with the addition of starch is shown in FIGS. 6 and 7 .
항산화도를 분석하기 위하여, 7mM ABTS와 2.4mM potassium persulfate를 녹인 후 암소에서 16시간 반응한 ABTS시약을 25배 희석하여 사용하였고, ABTS시약과 추출물을 넣고 혼합, 암소에서 7분간 반응 시킨 후 734nm에서 흡광도 측정하였다. ABTS 라디컬 소거능은 다음의 식으로 계산하였다. 각 추출물은 도 8a 내지 도 8d에 나타나 있다.To analyze the antioxidant level, after dissolving 7mM ABTS and 2.4mM potassium persulfate, the ABTS reagent reacted for 16 hours in the dark was diluted 25-fold, and the ABTS reagent and the extract were mixed and reacted in the dark for 7 minutes. Absorbance was measured. ABTS radical scavenging ability was calculated by the following equation. Each extract is shown in Figures 8A-8D.
실험결과, DSW에서는 팽화 후 시료의 ABTS 라디칼 소거능이 더 높고, 2DSW에서는 팽화 전 시료가 높은 것이 확인되었다 (도 9a 및 도 9b, 표 4 및 표 5).As a result of the experiment, it was confirmed that the ABTS radical scavenging ability of the sample after expansion was higher in DSW, and that the sample before expansion was high in 2DSW ( FIGS. 9A and 9B , Tables 4 and 5).
또한, DW에서는 팽화 전 시료가 ABTS라디칼 소거능이 더 높고, DW에 1 ∼50%(w/w) EtOH(70% 농도) 추가 첨가한 실험구에서는 팽화 후 시료의 상기 라디칼 소거능이 더 높은 것으로 확인되었다 (도 9c 및 도 9d, 표 6 및 표 7).In addition, in DW, the sample before swelling had higher ABTS radical scavenging ability, and in the experimental group in which 1-50% (w/w) EtOH (70% concentration) was added to DW, it was confirmed that the radical scavenging ability of the sample after swelling was higher (Fig. 9c and 9d, Table 6 and Table 7).
이하, 본 발명을 사용하기에 바람직하고 다양한 화장품 제형을 개시하지만 본 발명의 권리범위는 개시된 제형에 제한되지 않음은 물론이다.Hereinafter, various cosmetic formulations preferred for use in the present invention are disclosed, but it should be understood that the scope of the present invention is not limited to the disclosed formulations.
제조예1. 화장수Preparation Example 1. toilet water
본 발명에 따른 해양심층수 속새 추출물이 10% 함유된 화장수를 하기 표4과 같이 제조하였다.A lotion containing 10% of the deep-sea deep-sea water extract according to the present invention was prepared as shown in Table 4 below.
제조예2. 로션Preparation Example 2. Lotion
본 발명에 따른 해양심층수 속새 추출물이 10% 함유된 로션을 하기 표 9와 같이 제조하였다.A lotion containing 10% of the deep-sea serrata extract according to the present invention was prepared as shown in Table 9 below.
제조예3. 크림Preparation Example 3. cream
본 발명에 따른 해양심층수 속새 추출물이 10% 함유된 크림을 하기 표 10과 같이 제조하였다.A cream containing 10% of the deep-sea water serrata extract according to the present invention was prepared as shown in Table 10 below.
제조예4. 에센스 제조Preparation Example 4. Essence Manufacturing
본 발명에 따른 해양심층수 속새 추출물이 10% 함유된 에센스를 하기 표 11와 같이 제조하였다.An essence containing 10% of the deep-sea water serrata extract according to the present invention was prepared as shown in Table 11 below.
이상의 설명으로부터, 본 출원이 속하는 기술분야의 당업자는 본 출원이 그 기술적 사상이나 필수적 특징을 변경하지 않고서 다른 구체적인 형태로 실시될 수 있다는 것을 이해할 수 있을 것이다. 이와 관련하여, 이상에서 기술한 실시예들은 모든 면에서 예시적인 것이며 한정적인 것이 아닌 것으로 이해해야만 한다. 본 출원의 범위는 상기 상세한 설명보다는 후술하는 특허 청구범위의 의미 및 범위 그리고 그 등가 개념으로부터 도출되는 모든 변경 또는 변형된 형태가 본 출원의 범위에 포함되는 것으로 해석되어야 한다.From the above description, those skilled in the art to which the present application pertains will be able to understand that the present application may be embodied in other specific forms without changing the technical spirit or essential characteristics thereof. In this regard, it should be understood that the embodiments described above are illustrative in all respects and not restrictive. The scope of the present application should be construed as including all changes or modifications derived from the meaning and scope of the claims to be described later rather than the above detailed description and their equivalent concepts to be included in the scope of the present application.
Claims (6)
상기 단계에서 수득된 속새 팽화물에 농도 70%의 주정 1 ∼ 50%(w/w)를 추가로 더 첨가한 해양심층수로 가열 환류 추출하는 단계로 이루어진 것이 특징인 해양심층수 속새 추출물의 제조방법.
Sprinkling deep sea water on the dried sagebrush sample to obtain popped prunus spp. for 7 minutes at an internal temperature of 180° C. of the expander;
A method for producing an extract of deep-sea water, characterized in that it comprises the step of heating and refluxing the deep-sea water in which 1-50% (w/w) of alcohol having a concentration of 70% is further added to the expanded horseradish obtained in the above step.
An extract from deep-sea water produced by the method of claim 1 .
An antioxidant functional cosmetic composition comprising the extract of claim 4 as an active ingredient.
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