KR102314895B1 - Method for manufacturing rice grain size bean curd with improved nutritional, hygiene and storage properties, and rice grain size bean curd manufactured by this method - Google Patents

Method for manufacturing rice grain size bean curd with improved nutritional, hygiene and storage properties, and rice grain size bean curd manufactured by this method Download PDF

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KR102314895B1
KR102314895B1 KR1020210058235A KR20210058235A KR102314895B1 KR 102314895 B1 KR102314895 B1 KR 102314895B1 KR 1020210058235 A KR1020210058235 A KR 1020210058235A KR 20210058235 A KR20210058235 A KR 20210058235A KR 102314895 B1 KR102314895 B1 KR 102314895B1
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tofu
size
rice
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leaves
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오명석
임은주
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엠플러스에프엔씨(주)
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L11/00—Pulses, i.e. fruits of leguminous plants, for production of food; Products from legumes; Preparation or treatment thereof
    • A23L11/40—Pulse curds
    • A23L11/45—Soy bean curds, e.g. tofu
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L11/00—Pulses, i.e. fruits of leguminous plants, for production of food; Products from legumes; Preparation or treatment thereof
    • A23L11/05—Mashed or comminuted pulses or legumes; Products made therefrom
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/02—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by heating materials in packages which are progressively transported, continuously or stepwise, through the apparatus
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
    • A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
    • A23L3/3463—Organic compounds; Microorganisms; Enzymes
    • A23L3/3472—Compounds of undetermined constitution obtained from animals or plants
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/36—Freezing; Subsequent thawing; Cooling
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
    • A23P10/00—Shaping or working of foodstuffs characterised by the products
    • A23P10/20—Agglomerating; Granulating; Tabletting
    • A23P10/22—Agglomeration or granulation with pulverisation of solid particles, e.g. in a free-falling curtain
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2250/00—Food ingredients
    • A23V2250/20—Natural extracts
    • A23V2250/21—Plant extracts
    • A23V2250/2116—Flavonoids, isoflavones
    • A23V2250/21168—Quercetin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2250/00—Food ingredients
    • A23V2250/20—Natural extracts
    • A23V2250/21—Plant extracts
    • A23V2250/2136—Phytosterols, phytostanols

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Abstract

The present invention relates to a method for manufacturing tofu of a rice grain size with improved nutrient, hygiene, and storage properties and tofu of a rice grain size manufactured thereby. The method for manufacturing tofu of a rice grain size comprises: (a) a step of washing beans; (b) a step of soaking the beans in fresh water for 8-14 hours at 10-18℃; (c) a step of extracting the soaked beans and grinding the same into 50-100 mesh sizes; (d) a step of increasing an amount of the grinded beans by heating the bean at 100-120℃ for 30 minutes to 2 hours; (e) a step of passing the heated beans through a sieve of 150-300 mesh to separate pureed soybean soup and bean water; (f) a coagulation step of putting the bean water passing through the sieve in a bucket, stirring the same with a stirring device, and adding a coagulant, vitamin leaf powder, and a hydrothermal extract of kurapi and pumpkin leaves; (g) a step of molding tofu in a constant shape; (h) a step of cutting the molded tofu in a unit shape; (i) a step of grinding the cut tofu to the rice grain size; (j) a step of frying the grinded tofu of the rice grain size in a frying pan; and (k) a packing step of putting the fried tofu of the rice grain size in a packing case.

Description

영양성분, 위생성 및 보관성이 개선된 쌀알 크기의 두부 제조방법 및 이 방법으로 제조된 쌀알 크기의 두부{Method for manufacturing rice grain size bean curd with improved nutritional, hygiene and storage properties, and rice grain size bean curd manufactured by this method}A method for manufacturing rice grain size bean curd with improved nutritional, hygiene and storage properties, and rice grain size bean curd manufactured by this method manufactured by this method}

본 발명은 영양성분, 위생성 및 보관성이 개선된 쌀알 크기의 두부 제조방법 및 이 방법으로 제조된 쌀알 크기의 두부에 관한 것이다.The present invention relates to a method for producing grain-sized tofu with improved nutritional components, hygiene and storage, and to a grain-sized tofu produced by the method.

일반적으로 콩은 단백질 35∼40% 지방 15∼20% 탄수화물 30% 가량으로 구성되어 있으며, 식이섬유, 비타민, 무기질 등이 들어 있는 영양식품으로, 20세기 들어 동서양을 막론하고 생산과 이용 면에서 세계 최고의 웰빙 식품으로 부상하였으며, 21세기에도 여전히 주목받는 밀레니엄 식품 중 하나이다.In general, soybeans are composed of 35-40% protein, 15-20% fat, and 30% carbohydrate. As a nutritious food containing dietary fiber, vitamins, and minerals, in the 20th century, both East and West, It has emerged as the best well-being food and is one of the millennial foods that are still attracting attention in the 21st century.

콩에 들어 있는 단백질의 양은 농작물 중에서 최고이고, 구성 아미노산의 종류도 육류에 비해 손색이 없어, 육류 대용식품으로 많이 이용되고 있으며, 특히 우리나라에서는 콩 제품으로 콩나물, 두부, 된장 등을 많이 먹는다.The amount of protein contained in soybeans is the highest among agricultural crops, and the type of amino acids is comparable to meat, so it is widely used as a meat substitute.

그 중 두부는 콩 속에 들어 있는 단백질을 추출하여 무기염류로 응고시킨 식품으로, 콩제품 가운데 가장 대중적인 가공품임과 동시에 양질의 식물성 단백질이 풍부한 식품이다.Among them, tofu is a food obtained by extracting the protein contained in soybeans and coagulating it with inorganic salts. It is the most popular processed product among soybean products and is rich in high-quality vegetable protein.

콩은 약 40％의 단백질을 함유하고 있어서 영양이 우수하나 소화율이 좋지 않아 볶거나 쪄서 먹을 경우에도 소화율은 50∼70%에 지나지 않는데 비해, 두부는 콩에 들어 있는 단백질의 93％ 이상, 탄수화물의 85％ 이상, 지방의 95％ 이상, 비타민의 50∼60% 이상을 함유하면서 소화율이 95％나 되므로 건강식품에 많이 사용되고 있다.Soybeans contain about 40% protein, so they have excellent nutrition, but their digestibility is poor, so even when eaten fried or steamed, the digestibility is only 50-70%. It contains more than 85%, more than 95% of fat, and more than 50-60% of vitamins, and has a digestibility of 95%, so it is widely used in health food.

최근에는 '웰빙 문화'가 자리잡으면서 아침식사나 간식용으로 간편하게 즐길 수 있는 떠먹는 두부가 다양하게 출시되고 있는데, 떠먹는 두부는 국, 찌개용 또는 반찬으로 떠올리던 기존 두부와 달리 간식이나 디저트로 깔끔하게 떠먹을 수 있도록 출시된 제품이다.Recently, with the establishment of a 'well-being culture', various types of tofu that can be easily enjoyed for breakfast or snack are being released. It is a product that has been released so that you can eat it cleanly.

이와 같이, 다양한 두부가 개발 및 공급되고 있으나, 남녀노소 모두에게 더 많은 선택을 받기 위해서는 영양성분, 위생성 및 보관성 등에서 더 많은 개선이 요구되고 있다. As such, a variety of tofu has been developed and supplied, but in order to receive more choices from men and women of all ages, more improvements are required in nutritional components, hygiene, and storage.

즉, 두부는 풍부한 단백질을 공급할 수 있으나, 이와 더불어 인체에 유익한 비타민 등의 성분을 함께 공급할 수 있다면 더 완벽한 웰빙 식품이 될 수 있을 것으로 예상된다. 또한, 두부는 공기 중에서 쉽게 색이 변하며 세균 등에 오염되기 쉬운 외관을 가지므로, 항균 기능에 의해 위생성을 강화시키면 더 많은 수요를 유도할 수 있을 것으로 예상된다. 또한, 두부는 저장 기간이 짧으므로, 저장 기간을 늘릴 수 있다면 더 많은 소비를 창출할 수 있을 것으로 예상된다.In other words, tofu can supply abundant protein, but it is expected that it will be a more perfect well-being food if it can also supply ingredients such as vitamins beneficial to the human body. In addition, since tofu easily changes color in the air and has an appearance that is easy to be contaminated with bacteria, etc., it is expected that more demand will be induced if hygiene is strengthened by the antibacterial function. In addition, since tofu has a short storage period, it is expected that more consumption can be created if the storage period can be extended.

대한민국 공개특허공보 제10-2016-0047372호Republic of Korea Patent Publication No. 10-2016-0047372

본 발명은 종래기술의 상기와 같은 문제점을 해결하기 위하여 안출된 것으로서, 영양성분, 위생성 및 보관성이 개선된 쌀알 크기의 두부 제조방법 및 이 방법으로 제조된 쌀알 크기의 두부를 제공하는 것을 목적으로 한다.The present invention has been devised to solve the above problems of the prior art, and it is an object of the present invention to provide a method for producing grain-sized tofu with improved nutritional components, sanitation and storage, and a method for preparing tofu of grain size of rice produced by this method. do.

본 발명은 the present invention

(a) 콩을 세척하는 단계;(a) washing the beans;

(b) 콩을 담수시키고, 섭씨 10~18℃로 유지시키면서 8~14시간 동안 불리는 단계;(b) soaking the soybeans for 8-14 hours while keeping the temperature at 10-18 ° C.;

(c) 불린콩을 건져서 50~100메시의 크기로 분쇄하는 단계;(c) removing the soaked beans and pulverizing them to a size of 50 to 100 mesh;

(d) 분쇄된 콩을 100~120℃로 30분~2시간 동안 가열하여 증자시키는 단계;(d) heating the pulverized soybeans at 100-120° C. for 30 minutes to 2 hours to steam;

(e) 가열되어 공급되는 콩을 150~300메시의 채에 통과시키면서 비지와 콩물을 분리시키는 단계;(e) passing the heated and supplied beans through a sieve of 150 to 300 mesh to separate okara and soybean water;

(f) 상기 채를 통과한 콩물을 버킷에 넣고, 교반기로 교반시키면서 응고제, 비타민 나뭇잎 분말, 및 쿠라피아잎 및 호박잎 열수추출물을 첨가하는 응고단계;(f) a coagulation step of adding a coagulant, vitamin leaf powder, and hot water extract of curaffia leaf and pumpkin leaf into a bucket of soybean water passed through the sieve, and stirring with a stirrer;

(g) 두부를 일정한 모양으로 성형하는 단계;(g) forming the tofu into a certain shape;

(h) 성형된 두부를 단위 모양으로 절단하는 단계;(h) cutting the molded tofu into a unit shape;

(i) 절단된 두부를 쌀알 크기로 파쇄하는 단계;(i) crushing the cut tofu to the size of rice grains;

(j) 파쇄된 쌀알 크기의 두부를 프라이팬에 볶는 단계; 및(j) frying tofu the size of crushed rice grains in a frying pan; and

(k) 볶은 쌀알 크기의 두부를 포장케이스에 담는 포장단계;를 포함하는 영양성분, 위생성 및 보관성이 개선된 쌀알 크기의 두부 제조방법을 제공한다.(k) a packaging step of putting the roasted grain-sized tofu in a packaging case; provides a method for producing tofu of the size of grains of rice with improved nutrition, hygiene and storage, including.

또한, 본 발명은Also, the present invention

상기 제조방법에 의해 제조된 영양성분, 위생성 및 보관성이 개선된 쌀알 크기의 두부를 제공한다.It provides a grain-sized tofu with improved nutritional components, hygiene and storage properties prepared by the above manufacturing method.

본 발명의 두부의 제조방법에 의하면, 영양성분, 위생성 및 보관성이 개선된 쌀알 크기의 두부를 손쉽게 고품질로 제조하는 것이 가능하다.According to the manufacturing method of the tofu of the present invention, it is possible to easily produce high-quality tofu of the size of a grain of rice with improved nutrition, hygiene and storage properties.

구체적으로 본 발명의 쌀알 크기의 두부의 제조방법에 의해 제조된 쌀알 크기의 두부는 비타민 나뭇잎 분말을 포함하므로, 단백질 이외에 퀘세틴, 캄페롤, 이소람네틴 등의 성분이 추가로 함유되며, 비타민E 및 플라보노이드 성분 함량이 강화되어 건강에 유익한 효능을 제공한다. Specifically, since the grain-sized tofu produced by the method for manufacturing rice-sized tofu of the present invention contains vitamin leaf powder, ingredients such as quercetin, camperol, and isoramnetin are additionally contained in addition to protein, and vitamin E and flavonoid component content is fortified to provide beneficial effects on health.

또한, 쿠라피아잎 및 호박잎 열수추출물을 포함함으로써, 영향성분이 강화되며, 항균 기능이 강화되어 우수한 위생성 및 보관성을 제공한다.In addition, by including the hot water extract of curaffia leaf and pumpkin leaf, the influence component is strengthened, and the antibacterial function is strengthened to provide excellent hygiene and storage.

도 1은 본 발명 쌀알 크기의 두부의 제조방법을 나타낸 플로 차트이다.
도 2는 본 발명 쌀알 크기의 두부 제조방법에 의해 제조된 쌀알 크기의 두부를 나타낸 도면이다.1 is a flowchart showing a method for manufacturing tofu of the size of rice grains of the present invention.
Figure 2 is a view showing the tofu of the grain size of rice prepared by the method for manufacturing the tofu of the present invention.

이하, 본 발명의 실시예와 도면을 참조하여 본 발명을 상세히 설명한다. 이들 실시예는 오로지 본 발명을 보다 구체적으로 설명하기 위해 예시적으로 제시한 것일 뿐, 본 발명의 범위가 이들 실시예에 의해 제한되지 않는다는 것은 당업계에서 통상의 지식을 가지는 자에 있어서 자명할 것이다.Hereinafter, the present invention will be described in detail with reference to embodiments and drawings of the present invention. It will be apparent to those of ordinary skill in the art that these examples are only presented by way of example to explain the present invention in more detail, and that the scope of the present invention is not limited by these examples. .

또한, 달리 정의하지 않는 한, 본 명세서에서 사용되는 모든 기술적 및 과학적 용어는 본 발명이 속하는 기술 분야의 숙련자에 의해 통상적으로 이해되는 바와 동일한 의미를 가지며, 상충되는 경우에는, 정의를 포함하는 본 명세서의 기재가 우선할 것이다.Further, unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs, and in case of conflict, this specification, including definitions description will take precedence.

도면에서 제안된 발명을 명확하게 설명하기 위해서 설명과 관계없는 부분은 생략하였으며, 명세서 전체를 통하여 유사한 부분에 대해서는 유사한 도면 부호를 붙였다. 그리고, 어떤 부분이 어떤 구성 요소를 "포함"한다고 할 때, 이는 특별히 반대되는 기재가 없는 한 다른 구성 요소를 제외하는 것이 아니라 다른 구성 요소를 더 포함할 수 있는 것을 의미한다. 또한, 명세서에서 기술한 "부"란, 특정 기능을 수행하는 하나의 단위 또는 블록을 의미한다.In order to clearly explain the invention proposed in the drawings, parts irrelevant to the description are omitted, and similar reference numerals are attached to similar parts throughout the specification. And, when a part "includes" a certain component, this means that other components may be further included, rather than excluding other components, unless otherwise stated. In addition, "unit" described in the specification means one unit or block that performs a specific function.

각 단계들에 있어 식별부호(제1, 제2, 등)는 설명의 편의를 위하여 사용되는 것으로 식별부호는 각 단계들의 순서를 설명하는 것이 아니며, 각 단계들은 문맥상 명백하게 특정 순서를 기재하지 않는 이상 명기된 순서와 다르게 실시될 수 있다. 즉, 각 단계들은 명기된 순서와 동일하게 실시될 수도 있고 실질적으로 동시에 실시될 수도 있으며 반대의 순서대로 실시될 수도 있다. 또한, 제1, 제2등의 용어는 다양한 구성요소들을 설명하는데 사용될 수 있지만, 상기 구성요소들은 상기 용어들에 의해 한정되어서는 안 된다. 상기 용어들은 하나의 구성요소를 다른 구성요소로부터 구별하는 목적으로만 사용된다.In each step, the identification number (first, second, etc.) is used for convenience of description, and the identification number does not describe the order of each step, and each step does not clearly describe a specific order in context. It may be performed differently from the order specified above. That is, each step may be performed in the same order as specified, may be performed substantially simultaneously, or may be performed in the reverse order. Also, terms such as first, second, etc. may be used to describe various elements, but the elements should not be limited by the terms. The above terms are used only for the purpose of distinguishing one component from another.

이하에서 본 발명을 자세하게 설명한다. Hereinafter, the present invention will be described in detail.

본 발명은 the present invention

(a) 콩을 세척하는 단계;(a) washing the beans;

(b) 콩을 담수시키고, 섭씨 10~18℃로 유지시키면서 8~14시간 동안 불리는 단계;(b) soaking the soybeans for 8-14 hours while keeping the temperature at 10-18 ° C.;

(c) 불린콩을 건져서 50~100메시의 크기로 분쇄하는 단계;(c) removing the soaked beans and pulverizing them to a size of 50 to 100 mesh;

(d) 분쇄된 콩을 100~120℃로 30분~2시간 동안 가열하여 증자시키는 단계;(d) heating the pulverized soybeans at 100-120° C. for 30 minutes to 2 hours to steam;

(e) 가열되어 공급되는 콩을 150~300메시의 채에 통과시키면서 비지와 콩물을 분리시키는 단계;(e) passing the heated and supplied beans through a sieve of 150 to 300 mesh to separate okara and soybean water;

(f) 상기 채를 통과한 콩물을 버킷에 넣고, 교반기로 교반시키면서 응고제, 비타민 나뭇잎 분말, 및 쿠라피아잎 및 호박잎 열수추출물을 첨가하는 응고단계;(f) a coagulation step of adding a coagulant, vitamin leaf powder, and hot water extract of curaffia leaf and pumpkin leaf into a bucket of soybean water passed through the sieve, and stirring with a stirrer;

(g) 두부를 일정한 모양으로 성형하는 단계;(g) forming the tofu into a certain shape;

(h) 성형된 두부를 단위 모양으로 절단하는 단계;(h) cutting the molded tofu into a unit shape;

(i) 절단된 두부를 쌀알 크기의 두부로 파쇄하는 단계;(i) crushing the cut tofu into tofu the size of a grain of rice;

(j) 파쇄된 쌀알 크기의 두부를 프라이팬에 볶는 단계; 및(j) frying tofu the size of crushed rice grains in a frying pan; and

(k) 볶은 쌀알 크기의 두부를 포장케이스에 담는 포장단계;를 포함하는 영양성분, 위생성 및 보관성이 개선된 쌀알 크기의 두부 제조방법에 관한 것이다.(k) a packaging step of putting the roasted grain-sized tofu in a packaging case; relates to a method for manufacturing tofu of the size of grains of rice with improved nutrition, hygiene and storage, including.

본 발명의 쌀알 크기의 두부 제조방법은 The method for producing tofu of the size of a grain of rice of the present invention is

(l) 상기 포장케이스에 담긴 쌀알 크기의 두부를 82~88℃온도의 살균수를 30~35분 동안 통과시키면서 살균시키는 단계;를 더 포함할 수 있다. (l) sterilizing the tofu of the size of a grain of rice contained in the packaging case while passing sterilizing water at a temperature of 82 to 88° C. for 30 to 35 minutes; may further include.

또한, 본 발명의 쌀알 크기의 두부 제조방법은 In addition, the method for producing tofu of the size of a grain of rice of the present invention is

(m) 상기 살균단계를 마친 포장케이스에 담긴 쌀알 크기의 두부를 섭씨 5~10℃의 온도로 30~35분 동안 1차 냉각시키고, 섭씨1~8℃의 온도로 60~80분 동안 2차 냉각시키는 냉각단계; 및(m) The tofu of the size of a grain of rice contained in the packaging case after the sterilization step is first cooled for 30 to 35 minutes at a temperature of 5 to 10 ° C., and secondly for 60 to 80 minutes at a temperature of 1 to 8 ° C. cooling step of cooling; and

(n) 상기 냉각단계를 마친 쌀알 크기의 두부에 포함되는 철 또는 비철을 검사하는 단계;를 더 포함할 수 있다. (n) the step of inspecting the iron or non-ferrous contained in the tofu of the size of a grain of rice after the cooling step; may further include.

본 발명의 일 실시형태에 있어서,In one embodiment of the present invention,

상기 비타민 나뭇잎 분말은 콩물 100 중량부에 대하여 0.2 내지 5 중량부로 포함될 수 있으며, 더욱 바람직하게는 0.3 내지 3 중량부로 포함될 수 있다. 상술한 범위로 비타민 나뭇잎 분말이 포함되는 경우, 쌀알 크기의 두부의 기본적인 성상 및 맛을 유지하면서도 비타민 나뭇잎에 포함된 분말퀘세틴, 캄페롤, 이소람네틴 성분으로 인하여 종래의 두부와 비교하여 더 많은 양의 비타민E 및 플라보노이드 등의 성분을 포함시킬 수 있다. 퀘세틴, 캄페롤 및 이소람네틴으로 이루어지는 플라보노이드 성분은, 일반적인 종래의 두부에서는 검출되지 않으며, 비타민 나뭇잎 분말이 0.2 중량부 포함되는 두부에서도 검출되지 않았지만, 비타민 나뭇잎 분말이 0.3 중량부 포함되는 쌀알 크기의 두부에서는 약 0.13mg/g정도 검출된다.The vitamin leaf powder may be included in an amount of 0.2 to 5 parts by weight based on 100 parts by weight of soybean water, more preferably 0.3 to 3 parts by weight. When the vitamin leaf powder is included in the above-mentioned range, it maintains the basic properties and taste of tofu the size of a grain of rice, but due to the powdered quercetin, camperol, and isoramnetin components contained in the vitamin leaves, more than the conventional tofu It may contain ingredients such as vitamin E and flavonoids in an amount. The flavonoid component consisting of quercetin, camphorol and isoramnetin was not detected in conventional tofu, and was not detected even in tofu containing 0.2 parts by weight of vitamin leaf powder, but the size of a grain of rice containing 0.3 parts by weight of vitamin leaf powder It is detected about 0.13 mg/g in the tofu of

비타민E는 일반 두부에서 약0.8mg/g 정도 검출되는 반면에, 비타민 나뭇잎 분말이 0.2 중량부 포함되는 경우에는 1.31mg/g 검출되었으며, 비타민 나뭇잎 분말이 0.3 중량부 포함되는 경우에는 1.38mg/g 검출된다. On the other hand, vitamin E was detected at about 0.8 mg/g in general tofu, 1.31 mg/g was detected when 0.2 parts by weight of vitamin leaf powder was included, and 1.38 mg/g when vitamin leaf powder was included in 0.3 parts by weight. detected.

본 발명의 일 실시형태에 있어서, 상기 쿠라피아잎 및 호박잎 열수추출물은 콩물 100 중량부에 대하여 1 내지 10 중량부로 포함될 수 있으며, 더욱 바람직하게는 2 내지 7 중량%로 포함될 수 있다. 상술한 범위로 열수추출물이 포함되는 경우, 쌀알 크기의 두부의 기본적인 성상 및 맛을 유지하면서도 두부에 유효한 항균 특성을 부여할 수 있다.In one embodiment of the present invention, the hot water extract of curaffia leaves and pumpkin leaves may be included in an amount of 1 to 10 parts by weight based on 100 parts by weight of soybean water, more preferably 2 to 7 parts by weight. When the hot water extract is included in the above range, it is possible to impart effective antibacterial properties to the tofu while maintaining the basic properties and taste of tofu the size of a grain of rice.

또한, 본 발명의 일 실시형태에 있어서, 상기 쿠라피아잎 및 호박잎 열수추출물은 쿠라피아잎 열수추출물 및 호박잎 열수추출물을 6:4 내지 9:1의 중량비로 혼합한 것일 수 있다. 그러나 이에 한정되는 것은 아니며, 쿠라피아잎 및 호박잎을 혼합하고, 여기에 물을 넣고 끓여서 제조할수 도 있다.Further, in one embodiment of the present invention, the hot water extract of curaffia leaves and pumpkin leaves may be a mixture of hot water extracts of curaffia leaves and hot water extracts of pumpkin leaves in a weight ratio of 6:4 to 9:1. However, the present invention is not limited thereto, and it may be prepared by mixing curaffia leaves and pumpkin leaves, adding water thereto, and boiling.

상기 쿠라피아잎 열수추출물 또는 호박잎 열수추출물은 세절한 쿠라피아잎 또는 호박잎에 대하여 2 내지 4배 중량의 물을 넣고, 전체부피가 1/1.8 내지 1/2.5이 될 때까지 끓이고, 여과하여 구라피아잎 또는 호박잎을 제거하고, 여과액을 다시 1/1.8 내지 1/2.5이 될 때까지 농축하여 제조할 수 있다.The hot water extract of Curapia leaves or hot water extracts of pumpkin leaves is added with 2 to 4 times the weight of water to the chopped curaffia leaves or pumpkin leaves, boiled until the total volume is 1/1.8 to 1/2.5, filtered, and filtered It can be prepared by removing the leaves or pumpkin leaves, and concentrating the filtrate until it becomes 1/1.8 to 1/2.5 again.

상기 쿠라피아(Krapia)는 과명이 마편초과이며, 학명이 Phyla nodiflora L로서, H. Kuramochi에 의해 일본에서 자생하는 필라 노디 플로라와 필라 카넨스를 교해하여 육종한 식물로 토양의 피복력과 월동력이 뛰어나고, 지면에 낮게 깔려 자라는 식물로 알려져 있다. 본 발명자들은 상기 쿠라피아잎의 열수추출물의 항균 활성을 연구한 바, 매우 우수한 항균 활성을 확인하였다.The Krapia is a phyla nodiflora L, whose scientific name is Phyla nodiflora L, and is a plant bred by H. Kuramochi by cultivating the phyla nodi flora and phyla canens, which are native to Japan, and has excellent soil coverage and wintering power. , is known as a plant that grows low on the ground. The present inventors studied the antibacterial activity of the hot water extract of the curaffia leaf, and confirmed very excellent antibacterial activity.

호박잎은 식이섬유소와 비타민이 풍부하고 칼로리는 낮아, 다이어트와 피부미용에 좋으며, 베타카로틴 성분이 함유되어 암을 예방하며, 루테인 성분이 들어있어 눈의 피로를 완화해주며, 특히 칼륨도 풍부해서 혈관을 깨끗하게 해주고 콜레스테롤 수치를 낮추는 것으로 알려져 있다. Pumpkin leaves are rich in dietary fiber and vitamins, low in calories, good for diet and skin care, contain beta-carotene to prevent cancer, and lutein to relieve eye fatigue. It is known to cleanse and lower cholesterol levels.

또한, 항산화 효능도 있어, 노화 방지는 물론 성인병을 예방하며, 피로 해소와 구강 건강에 좋으며, 손발이 차거나 빈혈이 있는 사람에게 좋은 것으로 알려져 있다. In addition, it has an antioxidant effect, prevents aging as well as adult diseases, relieves fatigue and is good for oral health, and is known to be good for people with cold hands and feet or anemia.

본 발명자들은 상기와 같은 호박잎의 효능에 주목하여 연구하던 중 호박잎 열수추출물이 우수한 항균 활성을 갖는 것을 발견하여 본 발명을 완성하였다. The present inventors have completed the present invention by discovering that the hot water extract of pumpkin leaves has excellent antibacterial activity while paying attention to the efficacy of pumpkin leaves as described above.

본 발명의 두부의 제조방법에 있어서, 상기에서 기술된 특징들을 제외하고는 이 분야에 공지된 방법을 제한없이 투여하여 두부를 제조할 수 있다. 예를 들어, 본 발명자들이 선출원한 대한민국 등록특허 10-1732182에 기재된 제조방법은 본 발명에 그대로 적용될 수 있다. In the method for producing tofu of the present invention, except for the features described above, the method known in the art may be administered without limitation to manufacture tofu. For example, the manufacturing method described in Korean Patent Registration No. 10-1732182 previously applied by the present inventors can be directly applied to the present invention.

이하에서, 실시예를 통하여 본 발명을 보다 상세히 설명한다. 그러나, 하기의 실시예는 본 발명을 더욱 구체적으로 설명하기 위한 것으로서, 본 발명의 범위가 하기의 실시예에 의하여 한정되는 것은 아니다. 하기의 실시예는 본 발명의 범위 내에서 당업자에 의해 적절히 수정, 변경될 수 있다. Hereinafter, the present invention will be described in more detail through examples. However, the following examples are provided to illustrate the present invention in more detail, and the scope of the present invention is not limited by the following examples. The following examples can be appropriately modified and changed by those skilled in the art within the scope of the present invention.

제조예 1: 쿠라피아잎 열수추출물의 제조Preparation Example 1: Preparation of hot water extract of curaffia leaves

세절한 쿠라피아(Kurapia)잎 1 kg 및 물 3 리터를 추출용기에 넣고, 전체부피가 2 리터가 될 때까지 끓여주었다. 다음으로 여과하여 추출액을 분리한 후, 다시 부피가 1/2이 될 때까지 농축하여 최종적으로 쿠라피아 열수추출물을 제조하였다.1 kg of chopped Kurapia leaves and 3 liters of water were placed in an extraction container, and boiled until the total volume was 2 liters. Next, the extract was separated by filtration, and then concentrated again until the volume was reduced to 1/2 to finally prepare a hot water extract of Curapia.

제조예 2: 호박잎 열수추출물의 제조Preparation Example 2: Preparation of hot water extract of pumpkin leaves

세절한 호박잎 1 kg 및 물 3 리터를 추출용기에 넣고, 전체부피가 2 리터가 될 때까지 끓여주었다. 다음으로 여과하여 추출액을 분리한 후, 다시 부피가 1/2이 될 때까지 농축하여 호박잎 열수추출물을 제조하였다.1 kg of chopped pumpkin leaves and 3 liters of water were placed in an extraction container, and boiled until the total volume was 2 liters. Next, the extract was separated by filtration, and then concentrated again until the volume was reduced to 1/2 to prepare a hot water extract of pumpkin leaves.

제조예 3: 쿠라피아잎 및 호박잎 열수추출물의 제조Preparation Example 3: Preparation of hot-water extracts from curaffia leaves and pumpkin leaves

상기 제조예 1에서 제조된 쿠라피아잎 열수추출물 및 제조예 2에서 제조된 호박잎 열수추출무을 7:3으로 혼합하여 쿠라피아잎 및 호박잎 열수추출물을 제조하였다.The hot water extract of curaffia leaves prepared in Preparation Example 1 and the hot water extract of pumpkin leaves prepared in Preparation Example 2 were mixed at a ratio of 7:3 to prepare hot water extracts of curaffia leaves and pumpkin leaves.

실시예 1: 쌀알 크기의 두부의 제조Example 1: Preparation of tofu the size of a grain of rice

콩을 수납하고 세척수를 분사하여 콩에 혼합된 이물질을 제거하면서 세척하였다. 세척된 콩을 담수시키고, 섭씨 10~18℃로 유지시키면서 10시간 동안 불렸다. 다음으로 불린 콩을 건져서 50~100메시로 분쇄하였다. 다음으로 분쇄된 콩을 100~120℃로 1시간 동안 가열하여 증자시키고, 150~300메시의 채에 통과시키면서 비지와 콩물을 분리시켰다. 상기 콩물을 버킷에 넣고, 교반기로 교반시키면서 응고제, 비타민 나뭇잎 분말(콩물 100 중량부에 대하여 1 중량부로 첨가), 및 상기 제조예 3에서 제조한 쿠라피아잎 및 호박잎 열수추출물(콩물 100 중량부에 대하여 3 중량부로 첨가)을 첨가하고, 응고시켰다. 다음으로 두부를 일정한 모양으로 성형하고, 성형된 두부를 단위 모양으로 절단하였다. 다음으로 절단된 두부를 파쇄기를 이용하여 쌀알 크기로 파쇄한 다음 파쇄된 쌀알 크기의 두부를 가스레인지 약불에서 5분 동안 프라이팬에 볶아 60-80%까지 수분을 제거한 후 볶은 쌀알 크기의 두부를 포장케이스에 담아 포장하였다.Soybeans were housed and washing water was sprayed to remove foreign substances mixed with beans. The washed soybeans were freshwater and soaked for 10 hours while maintaining the temperature at 10-18°C. Next, the soaked beans were removed and crushed into 50~100 mesh. Next, the crushed soybeans were heated to 100-120° C. for 1 hour to steam, and passed through a 150-300 mesh sieve to separate okara and soybean water. Put the soybean water in a bucket, and while stirring with a stirrer, a coagulant, vitamin leaf powder (added in 1 part by weight based on 100 parts by weight of soybean water), and hot water extracts of curaffia leaves and pumpkin leaves prepared in Preparation Example 3 (in 100 parts by weight of soybean water) 3 parts by weight) was added and solidified. Next, the tofu was molded into a certain shape, and the molded tofu was cut into a unit shape. Next, shred the cut tofu to the size of a grain of rice using a shredder, and then fry the tofu the size of a grain of rice in a frying pan over low heat on a gas stove for 5 minutes to remove 60-80% of the moisture. packed in.

이후, 상기 포장케이스에 담긴 쌀알 크기의 두부를 82~88℃온도의 살균수를 섭씨 30~35분 동안 통과시키면서 살균시킨 후, 포장케이스에 담긴 쌀알 크기의 두부를 섭씨 5~10℃의 온도로 30~35분 동안 1차 냉각시키고, 섭씨 1~8℃의 온도로 60~80분 동안 2차 냉각시켰다. 다음으로 쌀알 크기의 두부에 포함되는 철 또는 비철을 검사하고, 냉장시켜 보관하였다.Thereafter, the tofu of the size of a grain of rice contained in the packaging case was sterilized while passing sterilizing water at a temperature of 82 to 88° C. for 30 to 35 minutes, and then the tofu the size of a grain of rice contained in the packaging case was heated to a temperature of 5 to 10° C. First cooled for 30 to 35 minutes, and secondarily cooled for 60 to 80 minutes at a temperature of 1 to 8 ° C. Next, ferrous or non-ferrous contained in tofu the size of a grain of rice was tested and stored refrigerated.

실시예 2: 쌀알 크기의 두부의 제조Example 2: Preparation of tofu the size of a grain of rice

실시예 1과 동일한 방법으로 제조된 쌀알 크기의 두부를 프라이팬에 볶을 때 생란의 난백과 난황을 혼합한 혼합물(쌀알 크기의 두부 100 중량부에 대하여 10 내지 20 중량부로 첨가)을 넣어 실시예 1과 동일한 조건으로 볶은 후 포장 및 검사한 다음 냉장시켜 보관하였다.When the tofu of the size of a grain of rice prepared in the same way as in Example 1 is fried in a frying pan, a mixture of raw egg white and egg yolk (10 to 20 parts by weight is added based on 100 parts by weight of tofu the size of a grain of rice) is added to Example 1 After roasting under the same conditions, packaging and inspection were performed, and then stored refrigerated.

실시예 3: 쌀알 크기의 두부의 제조Example 3: Preparation of tofu the size of a grain of rice

콩을 수납하고 세척수를 분사하여 콩에 혼합된 이물질을 제거하면서 세척하였다. 세척된 콩을 담수시키고, 섭씨 10~18℃로 유지시키면서 10시간 동안 불렸다. 다음으로 불린 콩을 건져서 50~100메시로 분쇄하였다. 다음으로 분쇄된 콩을 100~120℃로 1시간 동안 가열하여 증자시키고, 150~300메시의 채에 통과시키면서 비지와 콩물을 분리시켰다. 상기 콩물을 버킷에 넣고, 교반기로 교반시키면서 황산칼슘이나 글루코노델타락톤 등의 순두부용 응고제, 비타민 나뭇잎 분말(콩물 100 중량부에 대하여 1 중량부로 첨가), 및 상기 제조예 3에서 제조한 쿠라피아잎 및 호박잎 열수추출물(콩물 100 중량부에 대하여 3 중량부로 첨가)을 첨가하고, 응고시켰다. 다음으로 응고된 순두부를 파쇄기를 이용하여 쌀알 크기로 파쇄한 다음, 파쇄된 쌀알 크기의 두부와 한천 분말(쌀알 크기의 두부 100 중량부에 대하여 10 내지 20 중량부로 첨가) 및 생란의 난백과 난황을 혼합한 혼합물(쌀알 크기의 두부 100 중량부에 대하여 10 내지 20 중량부로 첨가)을 혼합하여 가스레인지 약불에서 5분 동안 프라이팬에 볶아 60-80%까지 수분을 제거한 후 볶은 쌀알 크기의 두부를 포장케이스에 담아 포장하였다. 이 후 과정은 실시예 1과 동일한 조건으로 살균 냉각한 다음 철 또는 비철 검사 후 냉장시켜 보관하였다.Soybeans were housed and washing water was sprayed to remove foreign substances mixed with beans. The washed soybeans were freshwater and soaked for 10 hours while maintaining the temperature at 10-18°C. Next, the soaked beans were removed and crushed into 50~100 mesh. Next, the crushed soybeans were heated to 100-120° C. for 1 hour to steam, and passed through a 150-300 mesh sieve to separate okara and soybean water. Put the soybean water in a bucket, and while stirring with a stirrer, a coagulant for soft tofu such as calcium sulfate or glucono deltalactone, vitamin leaf powder (added in 1 part by weight based on 100 parts by weight of soybean water), and the curaffia prepared in Preparation Example 3 Hot water extracts of leaves and pumpkin leaves (added in 3 parts by weight based on 100 parts by weight of soybean water) were added and coagulated. Next, the solidified soft tofu is crushed to the size of a grain of rice using a crusher, and then crushed tofu and agar powder (added in 10 to 20 parts by weight based on 100 parts by weight of tofu the size of a grain of rice) and egg white and yolk of raw eggs Mix the mixed mixture (added in 10 to 20 parts by weight based on 100 parts by weight of tofu the size of a grain of rice) and fry in a frying pan over low heat on a gas stove for 5 minutes to remove moisture to 60-80% packed in. After this process, it was sterilized and cooled under the same conditions as in Example 1, and then refrigerated and stored after testing for ferrous or non-ferrous iron.

비교예 1: 쌀알 크기의 두부의 제조Comparative Example 1: Preparation of tofu the size of a grain of rice

상기 실시예 1에서 쿠라피아잎 및 호박잎 열수추출물을 첨가하지 않은 것을 제외하고는 실시예 1과 동일한 방법으로 쌀알 크기의 두부를 제조하였다. Tofu having the size of a grain of rice was prepared in the same manner as in Example 1, except that hot water extracts of curaffia leaves and pumpkin leaves were not added in Example 1.

시험예 1. 쿠라피아잎 및 호박잎 열수추출물의 안전성 확인Test Example 1. Confirmation of safety of hot-water extracts from curaffia leaves and pumpkin leaves

실험동물은 4주령 Sprague-Dawley종 수컷 흰쥐(Central Lab animal, Seoul, Korea)를 환경이 조절된 사육실(기온 20℃ 습도 55%, 12시간 dark/light cycle)에서 펠렛형 고형사료(Jeil animal feed Co., Daejeon, Korea)로 2주간 사육하여 평균 체중이 200g 정도로 성장한 6주령의 쥐를 사용하여 상기 쿠라피아잎 열수추출물(제조예 1 제조), 호박잎 열수추출물(제조예 2 제조), 및 쿠라피아잎 및 호박잎 열수추출물(제조예 3 제조)의 독성테스트를 실시하였다.The experimental animal was a 4-week-old Sprague-Dawley breed male rat (Central Lab animal, Seoul, Korea) in an environment-controlled breeding room (temperature 20℃, humidity 55%, 12 hours dark/light cycle) with pellet-type solid feed (Jeil animal feed). Co., Daejeon, Korea), using 6-week-old rats having grown to about 200 g in average body weight for 2 weeks, the hot water extract of Curapia leaves (Preparation Example 1), Hot water extract of pumpkin leaves (Preparation Example 2), and Kura Toxicity tests were performed on hot water extracts of pia leaves and pumpkin leaves (prepared in Preparation Example 3).

구체적으로, 상기 4주령의 쥐 각각 5 마리씩에게 상기 제조예 1, 제조예 2, 및 제조예 3의 열수추출물을 1일 30ml씩을 펠렛형 고형사료(Jeil animal feed Co., Daejeon, Korea)와 함께 4주간 급여하면서 독성을 평가하였다.Specifically, 30 ml of the hot water extract of Preparation Example 1, Preparation Example 2, and Preparation Example 3 was given to 5 rats each of the 4 weeks old each day with pellet-type solid feed (Jeil animal feed Co., Daejeon, Korea). Toxicity was evaluated while feeding for 4 weeks.

상기 독성평가결과, 실험에 참여한 쥐 중 한 마리도 폐사하지 않고, 10마리 모두 4주간 건강을 유지한 것으로 확인되었다.As a result of the toxicity evaluation, it was confirmed that none of the rats participating in the experiment died, and all 10 rats remained healthy for 4 weeks.

시험예 2: 항균활성 측정Test Example 2: Measurement of antibacterial activity

쿠라피아잎 열수추출물(제조예 1 제조); 호박잎 열수추출물(제조예 2 제조); 및 쿠라피아잎 및 호박잎 열수추출물(7:3, 제조예 3 제조)의 항균 활성을 다음과 같은 방법으로 평가하였다.Curapia leaf hot water extract (prepared in Preparation Example 1); Pumpkin leaf hot water extract (prepared in Preparation Example 2); And the antibacterial activity of the hot water extracts of curaffia leaves and pumpkin leaves (7:3, prepared in Preparation Example 3) were evaluated in the following way.

대표적인 식중독균(Bacillus cereus, Bacillus subtilis, Campylobacter jejuni, Escherichia coli, Escherichia coli O-157, Listeria monocytogenes, Pseudomonas aeruginosa, Staphylococcus aureus, Salmonella enteritidis, Salmonella thypimurium, Vibrio parahaemolyticus, Yersinia enterocolitica)을 사용하였다.Representative food poisoning bacteria (Bacillus cereus, Bacillus subtilis, Campylobacter jejuni, Escherichia coli, Escherichia coli O-157, Listeria monocytogenes, Pseudomonas aeruginosa, Staphylococcus aureus, Salmonella enteritidis, Salmonella thypimurium, Vibrio parahaemolyticus) were used.

상기 세균류를 멸균된 TSB 배지 10㎖에 배양물 50㎕를 접종하여 30℃에서 24±1 시간 계대배양하였다. 이 배양물의 농도는 microplate reader 흡수파장 655㎚에서 Abs 0.6±0.1이 유지되었다. 계대배양 한 실험균을 멸균증류수로 10-3배로 희석하여 원판확산법을 실시하였다. 즉, 희석균주를 멸균면봉에 묻혀 4㎜ 두께의 평판배지에 확산 도말하고 멸균된 8㎜(두께 1㎜) 여지를 올려 상기 제조예 1 내지 3의 추출물을 각각 50㎕를 주입하였다. 20분 정도 충분히 흡입시킨 후 30에서 24±1 시간 배양하고 생육환을 확인하였다. The bacteria were inoculated with 50 μl of the culture in 10 ml of sterile TSB medium and subcultured at 30° C. for 24±1 hours. The concentration of this culture was maintained at an Abs 0.6±0.1 at an absorption wavelength of 655 nm in a microplate reader. The subcultured test bacteria were diluted 10 -3 times with sterile distilled water, and the disc diffusion method was performed. That is, the diluted strain was put on a sterile cotton swab and spread spread on a 4 mm thick plate medium, and a sterilized 8 mm (thickness 1 mm) paper was raised and 50 μl of each of the extracts of Preparation Examples 1 to 3 was injected. After sufficient inhalation for about 20 minutes, culture was performed at 30°C for 24±1 hours, and growth rings were confirmed.

상기 제조예 1 내지 3의 추출물의 항균활성은 여지를 포함한 투명대의 크기를 측정하여 평가하고, 그 결과를 하기 표 1에 나타내었다.The antibacterial activity of the extracts of Preparation Examples 1 to 3 was evaluated by measuring the size of the translucency including filtrate, and the results are shown in Table 1 below.

Figure 112021052246679-pat00001
Figure 112021052246679-pat00001

㈜ a: ATCC American Type Culture Collection, KCTC 한국생명공학연구원 생물자원센터; KCCM (사)한국종균협회Co., Ltd. a : ATCC American Type Culture Collection, KCTC Korea Research Institute of Bioscience and Biotechnology Biological Resources Center; KCCM Korea Sprout Association

b: 투명대의 크기(직경㎜) b : size of transparent band (diameter mm)

상기 표 1에서 확인되는 바와 같이, 제조예 1에서 제조한 쿠라피아잎 열수추출물 및 제조예 2에서 제조한 호박잎 추출물은 모두 전체 세균류들에 대하여 우수한 항균활성을 나타냈다.As can be seen in Table 1, the hot water extract of curaffia leaf prepared in Preparation Example 1 and the pumpkin leaf extract prepared in Preparation Example 2 all showed excellent antibacterial activity against all bacteria.

특히, 제조예 3에서 제조된 쿠라피아잎 및 호박잎 열수추출물은 각각의 추출물보다 훨씬 뛰어난 항균활성을 나타냈다. In particular, the hot-water extracts of curaffia leaves and pumpkin leaves prepared in Preparation Example 3 showed much superior antibacterial activity than the respective extracts.

시험예 3: 쌀알 크기의 두부의 저장성 평가Test Example 3: Evaluation of storage properties of tofu the size of a grain of rice

실시예 1에서 제조된 쌀알 크기의 두부 및 비교예 1에서 제조된 쌀알 크기의 두부를, 섭씨 25도에서 습도가 70%인 조건으로 보관하면서, 곰팡이에 의한 부패가 발생하는 시간을 측정하고, 그 결과를 하기 표 2에 나타내었다.While the grain-sized tofu prepared in Example 1 and the grain-sized tofu prepared in Comparative Example 1 were stored under a condition of 70% humidity at 25 degrees Celsius, the time for decay due to mold was measured, and the The results are shown in Table 2 below.

Figure 112021088687385-pat00005
Figure 112021088687385-pat00005

상기 표 2에 나타낸 바와 같이, 항균 물질을 포함하지 않은 비교예 1의 쌀알 크기의 두부는 5일 후에 곰팡이의 발생이 확인되었다. 그러나, 쿠라피아잎 및 호박잎 열수추출물을 포함한 실시예 1 쌀알 크기의 두부의 경우 13일까지 곰팡이의 발생이 억제되어, 항균성 부여에 의해 저장성이 매우 현저하게 향상된 것을 확인할 수 있었다.As shown in Table 2, the tofu having the size of a grain of rice in Comparative Example 1, which did not contain an antibacterial substance, was confirmed to be moldy after 5 days. However, in the case of the tofu of the size of rice grains of Example 1 containing the hot water extracts of curaffia leaves and pumpkin leaves, the occurrence of mold was suppressed until 13 days, and it was confirmed that the storage properties were very remarkably improved by imparting antibacterial properties.

Claims (4)

(a) 콩을 세척하는 단계;
(b) 콩을 담수시키고, 섭씨 10~18℃로 유지시키면서 8~14시간 동안 불리는 단계;
(c) 불린콩을 건져서 50~100메시의 크기로 분쇄하는 단계;
(d) 분쇄된 콩을 100~120℃로 30분~2시간 동안 가열하여 증자시키는 단계;
(e) 가열되어 공급되는 콩을 150~300메시의 채에 통과시키면서 비지와 콩물을 분리시키는 단계;
(f) 상기 채를 통과한 콩물을 버킷에 넣고, 교반기로 교반시키면서 응고제, 비타민 나뭇잎 분말, 및 쿠라피아잎 및 호박잎 열수추출물을 첨가하는 응고단계;
(g) 두부를 일정한 모양으로 성형하는 단계;
(h) 성형된 두부를 단위 모양으로 절단하는 단계;
(i) 절단된 두부를 쌀알 크기로 파쇄하는 단계;
(j) 파쇄된 쌀알 크기의 두부를 프라이팬에 볶는 단계; 및
(k) 볶은 쌀알 크기의 두부를 포장케이스에 담는 포장단계;를 포함하고,
상기 비타민 나뭇잎 분말은 콩물 100 중량부에 대하여 0.2 내지 5 중량부로 포함되며, 상기 쿠라피아잎 및 호박잎 열수추출물은 콩물 100 중량부에 대하여 1 내지 10 중량부로 포함되고, 상기 쿠라피아잎 및 호박잎 열수추출물은 쿠라피아잎 열수추출물 및 호박잎 열수추출물을 6:4 내지 9:1의 중량비로 혼합하여 제조되며,
상기 쿠라피아잎 열수추출물 또는 호박잎 열수추출물은 세절한 쿠라피아잎 또는 호박잎에 대하여 2 내지 4배 중량의 물을 넣고, 전체부피가 1/1.8 내지 1/2.5이 될 때까지 끓이고, 여과하여 쿠라피아잎 또는 호박잎을 제거하고, 여과액을 다시 1/1.8 내지 1/2.5이 될 때까지 농축하여 제조된 영양성분, 위생성 및 보관성이 개선된 쌀알 크기의 두부 제조방법.(a) washing the beans;
(b) soaking the soybeans for 8-14 hours while maintaining the temperature at 10-18 ° C.;
(c) removing the soaked beans and pulverizing them to a size of 50 to 100 mesh;
(d) heating the pulverized soybeans at 100-120° C. for 30 minutes to 2 hours to steam;
(E) passing the heated and supplied beans through a sieve of 150 to 300 mesh to separate okara and soybean water;
(f) a coagulation step of adding a coagulant, vitamin leaf powder, and hot water extract of curaffia leaf and pumpkin leaf into a bucket of soybean water passed through the sieve, and stirring with a stirrer;
(g) forming the tofu into a certain shape;
(h) cutting the molded tofu into a unit shape;
(i) crushing the cut tofu to the size of rice grains;
(j) frying tofu the size of crushed rice grains in a frying pan; and
(k) a packaging step of putting tofu the size of roasted rice grains in a packaging case;
The vitamin leaf powder is included in an amount of 0.2 to 5 parts by weight based on 100 parts by weight of soybean water, and the curaffia leaf and pumpkin leaf hot water extract is included in 1 to 10 parts by weight based on 100 parts by weight of the soybean water, and the curaffia leaf and pumpkin leaf hot water extract It is prepared by mixing the hot water extract of Curaffia leaf and the hot water extract of pumpkin leaf in a weight ratio of 6:4 to 9:1,
The hot water extract of Curapia leaves or hot water extract of pumpkin leaves is added with 2 to 4 times the weight of water with respect to the chopped Curapia leaves or pumpkin leaves, boiled until the total volume is 1/1.8 to 1/2.5, filtered, and filtered. A method for producing tofu in the size of grains of rice with improved nutritional components, hygiene and storage, prepared by removing leaves or pumpkin leaves and concentrating the filtrate until it becomes 1/1.8 to 1/2.5 again. 제1항에 있어서,
(l) 상기 포장케이스에 담긴 쌀알 크기의 두부를 82~88℃온도의 살균수를 30~35분 동안 통과시키면서 살균시키는 단계;를 더 포함하는 영양성분, 위생성 및 보관성이 개선된 쌀알 크기의 두부 제조방법.According to claim 1,
(l) sterilizing the rice grain-sized tofu contained in the packaging case while passing sterilizing water at a temperature of 82 to 88° C. for 30 to 35 minutes; Tofu manufacturing method. 제2항에 있어서,
(m) 상기 살균단계를 마친 포장케이스에 담긴 쌀알 크기의 두부를 섭씨 5~10℃의 온도로 30~35분 동안 1차 냉각시키고, 섭씨1~8℃의 온도로 60~80분 동안 2차 냉각시키는 냉각단계; 및
(n) 상기 냉각단계를 마친 쌀알 크기의 두부에 포함되는 철 또는 비철을 검사하는 단계;를 더 포함하는 것을 특징으로 하는 영양성분, 위생성 및 보관성이 개선된 쌀알 크기의 두부 제조방법.3. The method of claim 2,
(m) The tofu of the size of a grain of rice contained in the packaging case after the sterilization step is first cooled for 30 to 35 minutes at a temperature of 5 to 10 ° C, and a second time for 60 to 80 minutes at a temperature of 1 to 8 ° C. cooling step of cooling; and
(n) examining the iron or non-ferrous contained in the tofu of the size of a grain of rice after the cooling step; A method for producing tofu of the size of a grain of rice with improved nutrition, hygiene and storage, characterized in that it further comprises. 제1항의 방법으로 제조된 영양성분, 위생성 및 보관성이 개선된 쌀알 크기의 두부.Tofu in the size of a grain of rice with improved nutrition, hygiene, and storage properties manufactured by the method of claim 1.
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