KR102279773B1 - Composition for controlling stored product insect comprising Tulipalin A as effective component - Google Patents
Composition for controlling stored product insect comprising Tulipalin A as effective component Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
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Abstract
Description
본 발명은 튜리팔린 A(Tulipalin A)를 유효성분으로 함유하는 저곡해충 방제용 조성물에 관한 것이다.The present invention relates to a composition for controlling low grain pests containing tulipalin A as an active ingredient.
메틸브로마이드(methyl bromide)는 검역 및 토양 해충 방제를 위해 50여 년간 사용되어온 만능 훈증제이나, 높은 인축독성 및 신선 농산물에 처리 시 브롬화물(bromide)의 잔류로 인해 채소가 무르거나 종자의 발아가 억제되는 등의 문제점이 있다. 또한, 1989년 제정된 몬트리올 의정서에 의거 오존층 파괴물질로 지정되어 전 세계적으로 검역 등 특수한 목적을 제외하고는 사용을 금지시키고 있으며, 이에 따라 메틸브로마이드를 대체하기 위해 에틸포메이트(ethyl formate, EF)와 포스핀(phosphine)을 훈증제로 주로 사용해왔다.Methyl bromide is a universal fumigant that has been used for over 50 years for quarantine and soil pest control. There are problems such as In addition, according to the Montreal Protocol enacted in 1989, it is designated as an ozone-depleting substance and is banned worldwide except for special purposes such as quarantine. Accordingly, ethyl formate (EF) is used to replace methyl bromide. and phosphine have been mainly used as fumigants.
현재, 한국에서 사용중인 EF 훈증제 (Vapormate, BOC Ltd., Australia)는 이산화탄소(83.3%)와 에틸포메이트(16.7%)가 혼합되어 있는 형태를 하고 있다. EF는 식물 휘발성 물질로 살충 효과를 가지고 있으며, 장점은 짧은 처리 시간(3-6 시간) 및 약제 잔류문제가 없다는 것이다. 단점은 침투력이 약하며, 식물 수착이 높고, 해충을 죽이기 위하여 높은 농도가 필요하며, 인화성이 있다는 것이다.Currently, the EF fumigant used in Korea (Vapormate, BOC Ltd., Australia) is in the form of a mixture of carbon dioxide (83.3%) and ethyl formate (16.7%). EF is a plant volatile material and has an insecticidal effect, and the advantages are short treatment time (3-6 hours) and no drug residue problem. Disadvantages include poor penetration, high plant sorption, high concentrations required to kill pests, and flammability.
또한, 포스핀은 현재까지 알려진 훈증 농약 중 검역전처리 등에서 사용 중인 가장 경제적이고 편리한 물질로 처리 후 잔류성이 없어 전 세계적으로 저곡해충 방제를 위해 양곡창고, 국화, 장미, 백화절화 등의 화훼류 등에 광범위하게 사용하였다. 그러나, 과채류의 경우 처리 시 딸기와 복숭아에서 변색증상이 나타났으며, 감에서는 빠른 성숙을 유발하고 바나나에서는 성숙을 지연시키는 등의 약해가 보고된 바 있으며, 저곡해충 방제 시에도 온도별 발생량이 일정치 않고, 훈증시설을 고려하지 않아 과도한 약량 처리로 인해 포스핀에 대한 저곡해충의 저항성이 45개 이상에서 보고되었다(Park et al., Environ Toxicol Pharmacol. 2008 Jan;25(1):121-6).In addition, phosphine is the most economical and convenient substance used in quarantine pretreatment among known fumigation pesticides. It has no residue after treatment, so it is widely used in cereal warehouses, chrysanthemums, roses, flowers such as cut flowers, etc. was used. However, in the case of fruits and vegetables, discoloration symptoms were observed in strawberries and peaches during treatment, and weak damage such as causing rapid maturation in persimmons and delaying maturation in bananas have been reported. The resistance of low-grain pests to phosphine was reported in more than 45 cases due to excessive drug treatment due to not taking into account the fumigation facility (Park et al ., Environ Toxicol Pharmacol. 2008 Jan;25(1):121-6) ).
높은 저항성을 갖는 가루개나무좀(Rhzopertha dominica)이 1997년 호주에서 보고되었으며, 현재는 주요 저곡해충 4종 쌀바구미(Sitophilus orzyae), 가루개나무좀, 거짓쌀도둑거저리(Tribolium castaneum) 및 갈색머리대장(Cryptolestes ferrugineus)에 대해 호주 동부지역을 중심으로 산발적으로 발생됨을 모니터링하고 있으며, 생산 농가의 소형 싸일로(silo)부터 대형 싸일로로 이동 보관 후 수출 직전까지 포스핀 저항성 저곡해충을 모니터링하고 있다. 또한, 중국의 양곡창고에서는 상기 저곡해충 4종에 대한 높고 낮은 수준의 포스핀 저항성이 분포한다고 보고되었다. Rhzopertha dominica with high resistance was reported in Australia in 1997, and now there are four major low-grain pests, the rice weevil ( Sitophilus orzyae ), the white rice weevil, Tribolium castaneum and the brown-haired large intestine ( Cryptolestes ferrugineus ) is monitored sporadically in the eastern part of Australia, and phosphine-resistant low-grain pests are monitored from small silos of production farms to large silos after storage and immediately before export. In addition, it has been reported that high and low levels of phosphine resistance to the four low-grain pests are distributed in grain warehouses in China.
저곡해충에 대한 포스핀 저항성은 다양한 형태로 발전되며, 고 약량(기준 이상) 투여 훈증 조건에서는 장기간 노출된 후 살아난 저곡해충이 약제에 대한 저항성이 증가할 수 있으며, 저 약량(기준 이하) 투여 훈증 조건에서는 저항성 저곡해충이 살아서 세대를 거듭하면서 저항성 개체가 증가될 수 있다. 밀폐가 잘 되는 훈증시설을 이용해 훈증 온도에 맞는 적절한 약량과 시간을 노출시키는 것이 포스핀 저항성을 제어할 수 중요한 방법이며, 저곡해충의 포스핀 저항성 여부의 판별 및 저항성 정도를 측정하여 적절한 방제 농도 및 훈증시간 전략을 세워야 방제가 가능하다고 보고되었다(Park et al., 2008).Phosphine resistance to low-grain pests develops in various forms, and resistance to drugs may increase in low-grain pests that survive long-term exposure under high-dose (above the standard) administration fumigation conditions. Under these conditions, resistant low-grain pests may survive and the number of resistant individuals may increase over generations. Using a well-sealed fumigation facility and exposing the appropriate dose and time for the fumigation temperature is an important method to control phosphine resistance. It has been reported that control is possible only when a fumigation time strategy is established (Park et al ., 2008).
튜리팔린 A (Tulipalin A; α-Methylene butyrolactone)는 튤립 구근에서 분리되는 물질로, 저용량의 튜리팔린 A는 T-세포와 같은 면역 세포의 기능에 영향을 미친다고 알려져 있다. 튜리팔린 A는 총채벌레류에 높은 사충율을 나타낸다는 보고되었다(Datta PK et al., Biosci Biotechnol Biochem. 1999;63(4):760-1; Kim CS et al., Biosci Biotechnol Biochem. 1998;62(8):1546-9). 튜리팔린 A는 튤립 구근에서 주로 분리되는 물질로 알려져 있지만, 김 등(1998)은 조팝나무(Spiraea prunifolia) 잎에서 튜리팔린 A를 분리했다고 보고했다. 또한, 조팝나무에서 분리된 튜리팔린 A 물질은 산가수분해(acidolysis)되어 튜리팔린 A 전구체로서 살충력을 보인다고 보고되었다. 이 물질은 국내에서 등록되거나 사용된 사례는 없으며, 처음 등록된 이후로 이 약제에 대한 사용은 없었다.Tulipalin A (α-Methylene butyrolactone) is a substance isolated from tulip bulbs. It is known that low doses of tulipalin A affect the functions of immune cells such as T-cells. It has been reported that turiphalin A exhibits a high mortality rate in thrips (Datta PK et al., Biosci Biotechnol Biochem. 1999;63(4):760-1; Kim CS et al., Biosci Biotechnol Biochem. 1998; 62(8):1546-9). Although turiphalin A is known to be mainly isolated from tulip bulbs, Kim et al. (1998) reported that they isolated turiphalin A from the leaves of Spiraea prunifolia. In addition, it has been reported that the turiphalin A material isolated from Meadowsemaceae is acid hydrolyzed and exhibits insecticidal activity as a turiphalin A precursor. This substance has not been registered or used in Korea, and there has been no use of this drug since it was first registered.
국내의 경우 신선 농산물에 처리가능한 다양한 훈증제 개발, 수입 후 곡물저장창고에 보관 중인 곡류의 저곡해충 방제 약제 개발 및 포스핀 저항성 정도에 따른 적절한 방제 농도 및 훈증시간을 적용한 신규 방제방법 도입이 필요한 실정이다.In the case of Korea, it is necessary to develop various fumigants that can be treated with fresh agricultural products, to develop low-grain pest control agents for grains stored in grain storage after importation, and to introduce a new control method that applies the appropriate control concentration and fumigation time according to the degree of phosphine resistance. .
한편, 한국등록특허 제0808490호에는 '에스-마이너스-튤리팔린비 또는 아세틸화된-에스-마이너스-튤리팔린비를 함유하는 조성물'이 개시되어 있고, 한국등록특허 제1818837호에는 '알릴 메르캅탄을 포함하는 저곡해충 방제용 조성물'이 개시되어 있으나, 본 발명의 '튜리팔린 A를 유효성분으로 함유하는 저곡해충 방제용 조성물'에 대해서는 기재된 바가 없다.On the other hand, Korean Patent No. 0808490 discloses 'a composition containing S-minus-tulipalin ratio or acetylated-S-minus-tulipalin ratio', and Korean Patent No. 1818837 discloses 'allyl mercaptan. Although the 'composition for controlling low grain pests comprising a' is disclosed, there is no description about the 'composition for controlling low grain pests containing turiphalin A as an active ingredient' of the present invention.
본 발명은 상기와 같은 요구에 의해 도출된 것으로서, 본 발명자들은 메틸 브로마이드와 포스핀과 같은 종래 저곡해충 방제 약제보다 해충을 보다 효과적으로 방제하며 약제 선택의 폭을 확대할 수 있는 방법을 제공하고자, 튜리팔린 A(Tulipalin A)의 훈증 방제 효과를 실험하였다. 포스핀 저항성 저곡해충에 20 ㎕/500 ㎖의 튜리팔린 A를 24시간 훈증처리한 결과, 거짓쌀도둑거저리, 쌀바구미 및 가루개나무좀의 성충에서 100% 사충률을 보이는 것을 확인함으로써, 본 발명을 완성하였다.The present invention was derived from the above needs, and the present inventors more effectively control pests than conventional low-grain pest control agents such as methyl bromide and phosphine. The fumigation control effect of Tulipalin A was tested. As a result of fumigating 20 μl/500 ml of turiphalin A to phosphine-resistant low-grain pests for 24 hours, it was confirmed that 100% mortality rate was observed in the adults of false rice thief mealworm, rice weevils and powdery mildew. completed.
상기 과제를 해결하기 위해, 본 발명은 튜리팔린 A(Tulipalin A) 또는 농약학적으로 허용가능한 이의 염을 유효성분으로 함유하는 포스핀(phosphine) 저항성 저곡해충 방제용 조성물을 제공한다.In order to solve the above problems, the present invention provides a composition for controlling phosphine-resistant low grain pests containing tulipalin A or an agrochemically acceptable salt thereof as an active ingredient.
또한, 본 발명은 상기 조성물을 저곡작물 또는 신선 농산물에 처리하는 단계를 포함하는, 포스핀 저항성 저곡해충의 방제 방법을 제공한다.In addition, the present invention provides a method for controlling phosphine-resistant low-grain pests, comprising the step of treating the composition to low-grain crops or fresh agricultural products.
본 발명의 튜리팔린 A 약제는 기존 사용되어오던 약제가 아닌 새롭게 사용하고자 하는 약제로, 소비자에게 약제 선택의 폭을 넓혀주며, 저곡해충의 방제에 효과적으로 대응하여 경제적 피해를 감소시킬 수 있다. 또한, 최근 국내 저장작물에 기존 약제인 포스핀(정제)에 내성을 가지는 해충이 출현하고 있어 새롭게 발명된 약제는 포스핀 저항성 저곡해충의 방제에 기여할 수 있다.The turiphalin A drug of the present invention is a drug to be used newly, rather than a drug that has been previously used, and it broadens the range of drug choices for consumers, and can effectively respond to the control of low-grain pests, thereby reducing economic damage. In addition, recently, pests resistant to phosphine (tablet), an existing drug, have appeared in domestic stock crops, so the newly invented drug can contribute to the control of phosphine-resistant low-grain pests.
도 1은 튜리팔린 A 훈증 처리를 위해 필요한 튜리팔린 A(Tulipalin A)의 실린더(가스 및 액상, a) 안전방지대(b) 및 기화기(c)의 모습이다.1 is a view of the cylinder (gas and liquid, a) safety guard (b) and vaporizer (c) of tulipalin A (Tulipalin A) required for fumigation treatment of turipalin A.
본 발명의 목적을 달성하기 위하여, 본 발명은 튜리팔린 A(Tulipalin A) 또는 농약학적으로 허용가능한 이의 염을 유효성분으로 함유하는 포스핀(phosphine) 저항성 저곡해충 방제용 조성물을 제공한다.In order to achieve the object of the present invention, the present invention provides a composition for controlling phosphine-resistant low grain pests containing tulipalin A or an agrochemically acceptable salt thereof as an active ingredient.
튜리팔린 A(Tulipalin A, α-methylene-α-butyrolactone)는 튤립과 알스트로메리아(Alstroemeria) 같은 특정 꽃에서 발견되는 자연적으로 발생하는 화합물이다. 튜리팔린 A는 알레르겐으로 꽃가루 등 흔히 접하는 사람에게서 직업성 접촉성 피부염(예: '튤립 손가락')을 일으키는 것으로 알려져 있다.Tulipalin A (α-methylene-α-butyrolactone) is a naturally occurring compound found in certain flowers such as tulips and Alstroemeria. Turifalin A is an allergen and is known to cause occupational contact dermatitis (eg 'tulip fingers') in people with common contact with pollen.
저곡해충은 저장 중의 곡식과 그 1차 가공품(밀가루, 정맥 등)에 해를 끼치는 해충군의 총칭으로, 우리나라에서 발생하는 저곡충은 그 종류가 많으나 주로 쌀바구미, 보리나방, 화랑곡명나방, 장두, 톱가슴머리대장, 쌀도둑, 거짓쌀도둑, 곡식수렁이 등이 있으며 그 중에서도 키가 크고 발생율이 큰 것은 쌀바구미, 보리나방, 화랑곡명나방 등이 있다.Low-grain pests are a generic term for a group of pests that harm the grain and its primary processed products (wheat, veins, etc.) during storage. There are many types of low-grain pests that occur in Korea, but mainly rice weevil, barley moth, Hwaranggok moth, and long bean. , saw chestnut head, rice thieves, false rice thieves, and grain worms. Among them, the tall and high incidence rate are rice weevil, barley moth, and Hwaranggok moth.
본 발명의 방제용 조성물에 있어서, 튜리팔린 A 화합물의 농약학적으로 허용 가능한 염은 예를 들어, 금속염, 유기 염기와의 염, 무기산과의 염, 유기산과의 염, 염기성, 또는 산성 아미노산과의 염 등이 포함될 수 있다. 바람직한 금속염의 일례로는 나트륨염 또는 칼륨염 등과 같은 알칼리 금속염; 칼슘염, 마그네슘염 또는 바륨염 등과 같은 알칼리 토금속염; 알루미늄염 등이 포함될 수 있다. 바람직한 유기 염기와의 염은 트리메틸아민, 트리에틸아민, 피리딘, 피콜린, 2,6-루티딘, 에탄올아민, 디에탄올아민, 트리에탄올아민, 시클로헥실아민, 디시클로헥실아민 또는 N,N-디벤질에틸렌디아민 등과의 염이 포함될 수 있다. 바람직한 무기산과의 염은 염산, 브롬화수소산, 질산, 황산 또는 인산 등과의 염이 포함될 수 있다. 바람직한 유기산과의 염은 포름산, 아세트산, 트리플루오로아세트산, 프탈산, 푸마르산, 옥살산, 타르타르산, 말레인산, 시트르산, 숙신산, 메탄술폰산, 벤젠술폰산 또는 p-톨루엔 술폰산 등과의 염이 포함될 수 있다. 바람직한 염기성 아미노산과의 염은 알기닌, 라이신 또는 오르니틴 등과의 염이 포함될 수 있다. 바람직한 산성 아미노산과의 염은 아스파르트산 또는 글루탐산 등과의 염이 포함될 수 있다.In the control composition of the present invention, the agrochemically acceptable salt of the turipalin A compound is, for example, a metal salt, a salt with an organic base, a salt with an inorganic acid, a salt with an organic acid, a basic or acidic amino acid salts and the like may be included. Examples of preferred metal salts include alkali metal salts such as sodium salt or potassium salt; alkaline earth metal salts such as calcium salts, magnesium salts or barium salts; Aluminum salts and the like may be included. Preferred salts with organic bases are trimethylamine, triethylamine, pyridine, picoline, 2,6-lutidine, ethanolamine, diethanolamine, triethanolamine, cyclohexylamine, dicyclohexylamine or N,N-di salts with benzylethylenediamine and the like may be included. Preferred salts with inorganic acids may include salts with hydrochloric acid, hydrobromic acid, nitric acid, sulfuric acid or phosphoric acid and the like. Preferred salts with organic acids may include salts with formic acid, acetic acid, trifluoroacetic acid, phthalic acid, fumaric acid, oxalic acid, tartaric acid, maleic acid, citric acid, succinic acid, methanesulfonic acid, benzenesulfonic acid or p-toluenesulfonic acid and the like. Preferred salts with basic amino acids may include salts with arginine, lysine, ornithine, and the like. Preferred salts with acidic amino acids may include salts with aspartic acid or glutamic acid and the like.
본 발명의 방제용 조성물에 있어서, 상기 포스핀 저항성 저곡해충은 거짓쌀도둑거저리(Tribolium castaneum), 쌀바구미(Sitophilus oryzae L.), 가루개나무좀(Rhizopertha dominica), 권연벌레(Lasioderma serricorne), 화랑곡나방(Indian mealmoth) 또는 보리나방(Sitotroga cerealella)일 수 있고, 바람직하게는 거짓쌀도둑거저리, 쌀바구미 또는 가루개나무좀일 수 있으나, 이에 제한되지 않는다.In the control for the compositions of the present invention, the phosphine pest resistance Ju Hu false rice thief geojeori (Tribolium castaneum), rice weevil (Sitophilus oryzae L.), one piece of wood over there.Sweet powder (Rhizopertha dominica), gwonyeon worm (Lasioderma serricorne), hwaranggok It may be a moth ( Indian mealmoth ) or barley moth ( Sitotroga cerealella ), and preferably may be false rice thief mealworm, rice weevil or powdery mildew, but is not limited thereto.
또한, 본 발명의 조성물은 훈증제, 훈연제, 연막제 또는 유제의 제형일 수 있고, 바람직하게는 훈증제의 제형일 수 있으나, 이에 제한되지 않는다. In addition, the composition of the present invention may be in the form of a fumigant, fumigant, smoke film or emulsion, preferably in the form of a fumigant, but is not limited thereto.
본 발명의 방제용 조성물에 함유된 유효성분의 함량은, 적용방법, 적용면적 및 공간, 적용부위 및 제형에 따라 적절히 조절할 수 있다.The content of the active ingredient contained in the control composition of the present invention can be appropriately adjusted according to the application method, application area and space, application site and formulation.
본 발명의 방제용 조성물은 또한, 상기 유효성분 이외에도 통상의 살충제, 살비제 또는 살균제와 혼합해서 사용될 수 있고, 이들 살충, 살균, 살비제에 사용되는 부형제 또는 담체를 더욱 포함할 수 있다. 상기 부형제 또는 담체는 사용용이성, 경제성, 제형 또는 살충효과 증진을 위한 통상의 물질일 수 있으며, 그 예로는 산화방지제, 분해촉진제, 무기필러 등이 있으나 이에 한정되는 것은 아니다. 상기 무기필러의 역할은 유기화합물인 살충성분을 담지 및 분산시켜 보다 효과적인 훈증이 이루어지도록 하는 역할을 수행한다. 상기 무기필러는 특별히 제한되는 것은 아니며 경제성이나 나머지 유기화합물과의 혼합 시 상용성 등을 고려하여 적절한 것을 선택하여 사용할 수 있다.The control composition of the present invention may also be used by mixing with conventional insecticides, acaricides or fungicides in addition to the active ingredients, and may further include excipients or carriers used in these pesticides, sterilizations, and acaricides. The excipient or carrier may be a conventional material for enhancing ease of use, economy, formulation or insecticidal effect, and examples thereof include, but are not limited to, antioxidants, decomposition accelerators, inorganic fillers, and the like. The role of the inorganic filler is to support and disperse the insecticidal component, which is an organic compound, so that more effective fumigation is achieved. The inorganic filler is not particularly limited, and an appropriate one may be selected and used in consideration of economic feasibility or compatibility when mixing with other organic compounds.
본 발명은 또한, 본 발명의 상기 방제용 조성물을 저곡작물 또는 신선 농산물에 처리하는 단계를 포함하는, 포스핀(phosphine) 저항성 저곡해충의 방제 방법을 제공한다.The present invention also provides a method for controlling phosphine-resistant low-grain pests, comprising the step of treating low-grain crops or fresh agricultural products with the control composition of the present invention.
상기 포스핀 저항성 저곡해충은 전술한 것과 같다.The phosphine-resistant low-grain pest is the same as described above.
본 발명에 따른 방제 방법에 있어서, 상기 처리는 저곡작물 또는 신선 농산물의 저장 창고에 직접 살포하거나 훈증소독하는 방법일 수 있고, 바람직하게는 훈증 처리하는 것일 수 있다. 상기 훈증처리는 튜리팔린 A의 사용시 안전 및 사용량이 확인가능한 안전방지대(저울 포함)와 약제가 충분히 기화될 수 있도록 기화기에 연결하여 처리할 수 있으나, 이에 제한되지 않는다.In the control method according to the present invention, the treatment may be a method of directly spraying or fumigating a storage warehouse of low-grain crops or fresh agricultural products, preferably fumigation treatment. The fumigation treatment can be processed by connecting a safety guard (including a scale) and a vaporizer so that the safety and usage amount can be confirmed when using turipalin A and a vaporizer so that the drug can be sufficiently vaporized, but is not limited thereto.
또한, 본 발명의 방제 방법은 본 발명의 방제용 조성물 외에, 방제 대상 해충에 대한 살충 활성을 높이기 위해 당업계에 사용되고 있는 약제와 병용하여 사용될 수 있다.In addition, the control method of the present invention, in addition to the control composition of the present invention, can be used in combination with a drug used in the art to increase the pesticidal activity against the pest to be controlled.
본 발명의 방제 방법에 있어서, 상기 튜리팔린 A의 처리 용량은 살충 효과가 최대로 될 수 있도록 대상 해충의 종류에 따라 처리 시간 또는 용량을 조절하여 처리할 수 있다.In the control method of the present invention, the treatment dose of turipalin A may be treated by adjusting the treatment time or dose according to the type of target pest so that the insecticidal effect can be maximized.
이하, 본 발명을 실시예에 의해 상세히 설명한다. 단, 하기 실시예는 본 발명을 예시하는 것일 뿐, 본 발명의 내용이 하기 실시예에 한정되는 것은 아니다.Hereinafter, the present invention will be described in detail by way of Examples. However, the following examples are merely illustrative of the present invention, and the content of the present invention is not limited to the following examples.
1. 공시충 준비1. Preparation of vaccinating insects
1) breeding dish(Φ 5.5 cm × 1.5 cm, SPL, Korea) 내 습도유지를 위해 탈지면과 여과지(filter paper)를 깐 후 1차 증류수로 적셔준다.1) To maintain humidity in the breeding dish (Φ 5.5 cm × 1.5 cm, SPL, Korea), lay out cotton wool and filter paper and wet it with primary distilled water.
2) 저곡해충의 먹이(밀가루+이스트+밀기울 또는 현미쌀)를 breeding dish에 제공한다.2) Provide food for low-grain pests (wheat + yeast + bran or brown rice) to the breeding dish.
3) 포스핀 저항성을 가지는 거짓쌀도둑거저리, 쌀바구미 및 가루개나무좀의 성충을 준비된 breeding dish에 30개체씩 넣는다.3) Put 30 specimens each of the adult phosphine-resistant false rice mealworm, rice weevils, and powdery mildew in the prepared breeding dish.
4) 상기 3)번의 breeding dish의 뚜껑을 덮고 밀봉테이프를 이용하여 뚜껑이 분리되지 않도록 밀폐한다.4) Cover the lid of the breeding dish in No. 3) above and seal it using sealing tape to prevent the lid from being separated.
2. 튜리팔린 A(Tulipalin A) 투약 준비 및 투약2. Tulipalin A (Tulipalin A) dosing preparation and administration
1) 상기에서 준비된 공시충이 접종된 breeding dish를 훈증상(12 L of desiccator (Duran, Germany))에 넣어준다.1) Put the breeding dish inoculated with the specimen prepared above in a fumigator (12 L of desiccator (Duran, Germany)).
2) 처리하는 약제의 가스 부피만큼 훈증상에서 가스를 빼낸다.2) Remove the gas from the fumigation chamber as much as the gas volume of the agent to be treated.
3) 가스 형태의 튜리팔린 A를 밀폐된 소규모 훈증상에 투약한다.3) Administer turiphalin A in gaseous form to a small, sealed fumigation.
4) 20℃에서 24시간 동안 처리한다.4) Treat at 20°C for 24 hours.
5) 처리 끝난 후, 훈증상을 개방한다.5) After treatment, open the fumigation chamber.
3. 약효조사3. Drug efficacy investigation
튜리팔린 A(Tulipalin A) 처리 후 훈증상 개방 1일 후, 성충의 사충률을 조사한다.After treatment with Tulipalin A, 1 day after opening the fumigation symptoms, the mortality rate of adults is investigated.
실시예 1. 저곡해충에 대한 튜리팔린 A(Tulipalin A) 방제 효과Example 1. Tulipalin A (Tulipalin A) control effect against low grain pests
포스핀 약제에 저항성을 보이는 거짓쌀도둑거저리, 쌀바구미, 가루개나무좀에 대해서 튜리팔린 A의 사충 효과를 분석하였다. 처리 온도는 20±1℃, 튜리팔린 A의 처리 용량은 1, 5 또는 20 ㎕/500 ㎖으로 하였으며, 총 처리 시간은 24시간으로 수행하였다. 그 결과, 하기 표 1에서 확인되는 것과 같이, 튜리팔린 A를 1 ㎕/500 ㎖의 용량으로 처리한 조건에서는 거짓쌀도둑거저리 및 쌀바구미에서 6.7% 사충률을 보였으며, 튜리팔린 A를 5 ㎕/500 ㎖의 용량으로 처리한 조건에서는 거짓쌀도둑거저리, 쌀바구미 및 가루개나무좀에서 각각 30%, 26.7% 및 10%의 사충률을 보였으며, 튜리팔린 A를 20 ㎕/500 ㎖의 용량으로 처리한 조건에서는 3종의 포스핀 저항성 저곡해충 모두에서 100%의 사충률을 보여주었다.The insecticidal effect of turiphalin A was analyzed on false rice weevils, rice weevils, and powdery mildew, which are resistant to phosphine drugs. The treatment temperature was 20±1° C., the treatment capacity of turiphalin A was 1, 5 or 20 μl/500 ml, and the total treatment time was 24 hours. As a result, as shown in Table 1 below, under the conditions of treatment with a dose of 1 μl/500 ml of turiphalin A, 6.7% mortality rate was observed in the false rice mealworm and rice weevils, and 5 μl of turiphalin A was In the condition treated with a dose of /500 ml, mortality rates of 30%, 26.7% and 10% were shown in false rice thief mealworm, rice weevils and powdery mildew, respectively, and turiphalin A was administered at a dose of 20 μl/500 ml. In the treated conditions, all three phosphine-resistant low-grain pests showed 100% mortality.
(㎕/500㎖)Volume
(μl/500ml)
(시간)processing period
(time)
상기의 결과를 통해, 본 발명의 튜리팔린 A는 다양한 저곡해충에 대해 효과적인 훈증 방제제로 사용될 수 있음을 확인할 수 있었다.Through the above results, it was confirmed that the turiphalin A of the present invention can be used as an effective fumigation control agent against various low grain pests.
Claims (4)
상기 포스핀 저항성 저곡해충은 거짓쌀도둑거저리(Tribolium castaneum), 쌀바구미(Sitophilus oryzae) 또는 가루개나무좀(Rhizopertha dominica)인 것을 특징으로 하는 방제용 조성물.As a composition for controlling phosphine-resistant low grain pests containing tulipalin A (Tulipalin A) or an agrochemically acceptable salt thereof as an active ingredient,
The phosphine-resistant low grain pests are false rice thief mealworm ( Tribolium castaneum ), rice weevil ( Sitophilus oryzae ) or powdery mildew ( Rhizopertha dominica ) Control composition, characterized in that.
상기 포스핀 저항성 저곡해충은 거짓쌀도둑거저리(Tribolium castaneum), 쌀바구미(Sitophilus oryzae) 또는 가루개나무좀(Rhizopertha dominica)인 것을 특징으로 하는 방제 방법.As a control method of low-grain pests comprising the step of treating the composition for controlling of claim 1 or 3 to low-grain crops or fresh agricultural products,
The phosphine-resistant low grain pests are false rice thief mealworm ( Tribolium castaneum ), rice weevil ( Sitophilus oryzae ) or powdery mildew ( Rhizopertha dominica ) Control method, characterized in that.
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| KR102295075B1 (en) * | 2021-06-22 | 2021-08-31 | 대한민국 | Composition for controlling agricultural pest comprising Tulipalin A as effective component |
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