KR102266729B1 - Composition for improving liver injury and liver disease comprising flower of Rosa rugosa Thunberg and Cinnamomum cassia PRESL - Google Patents
Composition for improving liver injury and liver disease comprising flower of Rosa rugosa Thunberg and Cinnamomum cassia PRESL Download PDFInfo
- Publication number
- KR102266729B1 KR102266729B1 KR1020190154616A KR20190154616A KR102266729B1 KR 102266729 B1 KR102266729 B1 KR 102266729B1 KR 1020190154616 A KR1020190154616 A KR 1020190154616A KR 20190154616 A KR20190154616 A KR 20190154616A KR 102266729 B1 KR102266729 B1 KR 102266729B1
- Authority
- KR
- South Korea
- Prior art keywords
- extract
- liver
- broiler
- composition
- ethanol
- Prior art date
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
- A61K36/738—Rosa (rose)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/54—Lauraceae (Laurel family), e.g. cinnamon or sassafras
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2300/00—Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Epidemiology (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Medical Informatics (AREA)
- Alternative & Traditional Medicine (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Gastroenterology & Hepatology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Medicines Containing Plant Substances (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
본 발명은 매괴화와 육계의 혼합추출물을 유효성분으로 함유하는 간손상 및 간질환 개선용 조성물에 관한 것이다.
본 발명의 매괴화와 육계의 혼합추출물을 함유하는 조성물은 혈중 알코올 농도를 낮추고, 아세트알데하이드 탈수소효소 활성을 높여 주며, 간조직을 보호하는 효과가 우수하며, 혈장 GOT, GPT, ALP, ALB, 총 단백질, 크레아틴 활성을 개선하며, 지질 축적과 염증을 개선하는 효과가 우수하다. The present invention relates to a composition for improving liver damage and liver disease, comprising a mixed extract of maemeekhwa and broiler as an active ingredient.
The composition containing the mixed extract of maechuhwa and broiler of the present invention lowers the blood alcohol concentration, increases the acetaldehyde dehydrogenase activity, has an excellent effect in protecting the liver tissue, and has excellent plasma GOT, GPT, ALP, ALB, total It improves protein and creatine activity, and has excellent effects on lipid accumulation and inflammation.
Description
본 발명은 매괴화와 육계의 혼합추출물을 이용한 간손상 및 간질환 개선용 조성물에 관한 것이다.The present invention relates to a composition for improving liver damage and liver disease using a mixed extract of broiler and broiler.
간은 인간의 신체장기 중 생체 내 대사가 가장 활발하게 일어나는 장기이다. 간은 소화계통으로 분류되지만 실제로는 탄수화물 대사, 아미노산 및 단백질 대사, 지방 대사, 담즙산 및 빌리루빈 대사, 비타민 및 무기질 대사, 호르몬 대사, 해독 작용 및 살균 작용 등 우리 몸에서 일어나는 거의 모든 일에 관여한다. The liver is the organ in which metabolism in the body occurs most actively among human body organs. Although the liver is classified as a digestive system, it is actually involved in almost everything that happens in our body, including carbohydrate metabolism, amino acid and protein metabolism, fat metabolism, bile acid and bilirubin metabolism, vitamin and mineral metabolism, hormone metabolism, detoxification and bactericidal action.
간은 인체 내 소화기계와 전신순환계 사이에 위치하면서 외부에서 들어온 생체 외 물질로부터 전신을 방어하는 기능을 수행하고 있다. 생체 내로 들어온 생체 외 물질은 일단 간을 통과하게 되므로 간은 영양소 이외에도 많은 독성물질에 노출될 위험이 다른 장기보다 많아 그만큼 손상될 확률도 매우 높다. 그러나 간은 재생능력이 우수한 장기로 약간의 손상이 있을 경우에는 충분히 정상으로 회복되지만, 손상이 지속될 경우에는 간 조직의 일부가 완전히 파괴되고 간 기능도 저하되는 등 정상 간으로의 회복이 어려운 상태가 된다. 이러한 간 손상이 만성화되면 그 원인에 상관없이 간 섬유화 또는 간경화, 간암으로 진행된다.The liver is located between the digestive system and the systemic circulatory system in the human body, and performs the function of defending the whole body from external substances introduced from the outside. Since ex vivo substances that enter the body pass through the liver once, the liver has a higher risk of being exposed to many toxic substances in addition to nutrients than other organs, so the probability of damage is very high. However, the liver is an organ with excellent regenerative capacity, and it recovers to normal if there is a slight damage, but if the damage continues, part of the liver tissue is completely destroyed and liver function is reduced. do. When such liver damage becomes chronic, it progresses to liver fibrosis, cirrhosis, or liver cancer regardless of the cause.
한편, 간 손상을 유발하는 원인으로는 스트레스성 만성피로, 지방성분이 포함된 음식 또는 알콜의 과다섭취, 바이러스의 감염, 각종 약품과 같은 유해물질, 영양부족 등 다양하다.On the other hand, there are various causes of liver damage, such as chronic stress-induced fatigue, excessive consumption of food or alcohol containing fat, viral infection, harmful substances such as various drugs, and nutritional deficiency.
이러한 간손상에 대한 치료효과가 우수하며, 대량 또는 장기간 투여시에도 부작용이 없는 천연물을 이용한 새로운 간질환 치료제의 개발이 요구된다.It is excellent in the therapeutic effect on such liver damage, and there is a need to develop a new therapeutic agent for liver disease using a natural product that does not have side effects even when administered in large quantities or for a long period of time.
매괴화는 장미과에 속하는 낙엽관목의 다년생인 해당화(Rosa rugosa Thunberg)는 막 피어난 꽃봉오리만 약재로 삼아 매괴화라 불리며, 열매, 잎, 꽃잎이 식용 가능하다. 해당화의 신선한 꽃에는 시트로넬롤(citronellol), 유제놀(eugenol), 네롤(renol), 퀘르세틴(quercetin), 탄닌(tannin), 베타카로틴(β-carotene), 시아닌(cyanin)이 함유되어 있으며, 열매에는 비타민 C(vitanin C), 사과산(malic acid), 퀴닌산(quinin acid) 등 다량의 당류와 비휘발산 성분이 함유된 것으로 알려져 있다(정보섭 및 신민교 저, 도해향약(생약)대사전, 영림사, pp 649-650, 1998). Rosa rugosa Thunberg , a perennial of a deciduous shrub belonging to the Rosaceae family, is called a plum flower, using only the bud that just bloomed as a medicinal plant, and its fruits, leaves and petals are edible. The fresh flowers of glycolysis contain citronellol, eugenol, nerol, quercetin, tannin, beta-carotene, and cyanin. The fruit is known to contain a large amount of sugars and non-volatile acids, such as vitamin C, malic acid, and quinin acid (by Jeong Bo-seop and Shin Min-gyo, Dohaehyangyak (Crude Medicine) Metabolism , Youngrimsa , pp 649-650, 1998).
매괴화는 짙은 향기가 있고 맛은 달고 쓰며 성질은 따듯하다. 매괴화는 간장과 비장에 작용하여 울체된 것을 풀어주며 흉복부가 그득하고 아픈 증상을 치료하며 기혈을 통하게 하여 생리, 생리 전 유방통, 타박상의 어혈 등에 사용되고, 담즙분비를 촉진하는 작용이 보고되었다. Maegaehwa has a strong scent, the taste is sweet and bitter, and the nature is warm. It has been reported to work on the liver and spleen to relieve congestion, treat chest and abdominal pain and painful symptoms, and use Qi-hyeol to pass through menstruation, breast pain before menstruation, bruises, etc., and to promote bile secretion.
육계는 녹나무과에 속하는 상록교목인 육계(Cinnamomum cassia PRESL = C. loureirii NEES)의 건피 또는 수피이며, 약재로 사용되는 수피에는 카사오일(cassa oil), 신남산(cinnamic acid), 신남알데하이드(cinnamic aldehyde)등 다량의 정유 성분 및 탄닌(tannin) 성분이 함유된 것으로 알려져 있다(정보섭 및 신민교 저, 도해향약(생약)대사전-약용식물편, 영림사, pp 453-455, 1998).Broiler is the dry skin or bark of Cinnamomum cassia PRESL = C. loureirii NEES, an evergreen tree belonging to the Camphoraceae family. ), etc., are known to contain a large amount of essential oil and tannins (by Jeong Bo-seop and Shin Min-gyo, Dohaehyangyak (crude medicine) Metabolism - Medicinal Plants ed ., Youngrimsa, pp 453-455, 1998).
본 발명은 천연약재인 매괴화와 육계를 혼합하여 추출한 혼합추출물을 사용하여 간손상과 간질환을 효과적으로 개선할 수 있는 조성물을 제공하는 것을 목적으로 한다.An object of the present invention is to provide a composition that can effectively improve liver damage and liver disease by using a mixed extract extracted by mixing maekaehwa and broiler chicken, which are natural medicines.
상기 목적을 달성하기 위하여, 본 발명은 매괴화와 육계의 혼합추출물을 유효성분으로 함유하는 간손상 및 간질환 개선용 조성물을 제공한다.In order to achieve the above object, the present invention provides a composition for improving liver damage and liver disease, containing a mixed extract of maegoehwa and broiler chicken as an active ingredient.
상기 조성물에서, 매괴화와 육계는 1:2 내지 2:1의 중량비로 혼합된 것임이 바람직하다.In the above composition, it is preferable that the maekgaehwa and the broiler chicken are mixed in a weight ratio of 1:2 to 2:1.
상기 조성물에서, 상기 추출물은 70% 에탄올을 추출용매로 사용하여 추출한 것임이 바람직하다. In the composition, the extract is preferably extracted using 70% ethanol as an extraction solvent.
상기 조성물에서, 상기 매괴화와 육계의 혼합추출물은,In the composition, the mixed extract of maekaehwa and broiler chickens,
매괴화와 육계의 혼합물을 8~12배(v/w)의 70% 에탄올에 넣어 55~65℃에서 20~28시간 동안 침지시키는 단계; 침지 후 추출액을 수득하는 단계; 수득한 추출액을 여과하는 단계; 및 여과된 추출액을 감압농축 및 건조시키는 단계를 포함하는 방법에 의하여 제조된 것임이 바람직하다.Putting the mixture of maemaehwa and broiler in 8-12 times (v/w) 70% ethanol and immersing it at 55-65° C. for 20-28 hours; obtaining an extract after immersion; filtering the obtained extract; And it is preferably prepared by a method comprising the step of concentrating and drying the filtered extract under reduced pressure.
본 발명의 매괴화와 육계의 혼합추출물을 함유하는 조성물은 급성 알코올 투여에 의한 간손상 유도 모델에서 확인되는 바와 같이 혈중 알코올 농도를 낮추고 알코올 대사과정에서 생성되는 독성물질인 아세트알데하이드를 분해하는 아세트알데하이드 탈수소효소 활성을 높여 주는 효과가 있다. As confirmed in the liver damage induction model by acute alcohol administration, the composition containing the mixed extract of maekgae and broiler chickens of the present invention lowers blood alcohol concentration and decomposes acetaldehyde, a toxic substance produced during alcohol metabolism. It has the effect of increasing the dehydrogenase activity.
또한 만성 알코올 투여에 의한 간손상 유도 모델에서 확인되는 바와 같이, 간조직에서 간세포 사이의 염증과 간세포 괴사와 비대 및 소포성 지방변성을 감소시키며, 만성 알코올섭취에 따른 간기능 지표인 혈장 GOT, GPT, ALP, ALB, 총 단백질, 크레아틴 활성에서도 우수한 개선효과가 나타났다. In addition, as confirmed in the liver injury induction model by chronic alcohol administration, it reduces inflammation between hepatocytes in liver tissue, hepatocellular necrosis, hypertrophy, and vesicular steato-degeneration. Plasma GOT, GPT, which are indicators of liver function according to chronic alcohol intake , ALP, ALB, total protein, and creatine activity also showed excellent improvement effects.
또한 비알코올성 지질 축적과 비알코올성 염증에 효과가 있으며, 고지방식이를 섭취한 만성 비알코올성 간손상 모델에서 간조직과 부고환조직을 보호하는 효과가 우수하다. In addition, it is effective in non-alcoholic lipid accumulation and non-alcoholic inflammation, and is excellent in protecting liver and epididymal tissues in a chronic non-alcoholic liver injury model fed a high-fat diet.
따라서 본 발명의 조성물은 다양한 원인으로 발생하는 간손상과 간질환의 개선에 효과적으로 사용될 수 있다. Therefore, the composition of the present invention can be effectively used to improve liver damage and liver disease caused by various causes.
도 1은 급성 알코올 투여에 의한 간손상 유도 모델에서 매괴화와 육계의 혼합비율 및 추출용매에 따라 추출물이 혈중 알코올 농도와 ALDH 활성에 미치는 효과를 비교한 결과이다.
도 2는 만성 알코올 투여에 의한 간손상 유도 모델에서 매괴화와 육계의 혼합추출물이 간조직과 간기능에 미치는 효과를 확인한 결과이다.
도 3은 매괴화와 육계의 혼합추출물이 비알코올성 지질축적에 미치는 효과를 확인한 결과이다.
도 4는 매괴화와 육계의 혼합추출물이 비알코올성 염증에 미치는 효과를 확인한 결과이다.
도 5는 매괴화와 육계의 혼합추출물이 고지방식이 섭취에 따른 간질환에 미치는 효과를 확인한 결과이다. 1 is a comparison result of the effect of the extract on blood alcohol concentration and ALDH activity according to the mixing ratio and extraction solvent of maekhwawa and broiler in a liver injury induction model by acute alcohol administration.
Figure 2 is the result of confirming the effect of the mixed extract of maemaekhwa and broiler on liver tissue and liver function in a liver injury induction model by chronic alcohol administration.
Figure 3 is the result of confirming the effect of the mixed extract of maekkeumhwa and broiler on the accumulation of non-alcoholic lipids.
Figure 4 is the result of confirming the effect of the mixed extract of maekkeehwa and broiler on non-alcoholic inflammation.
5 is the result of confirming the effect of the mixed extract of maekaehwa and broiler on liver disease according to the intake of a high-fat diet.
본 발명은 매괴화와 육계의 혼합추출물을 유효성분으로 함유하는 간손상 및 간질환 개선용 조성물에 관한 것이다.The present invention relates to a composition for improving liver damage and liver disease, comprising a mixed extract of maemeekhwa and broiler as an active ingredient.
매괴화와 육계는 1:2 내지 2:1의 중량비로 혼합하여 추출하는 것이 바람직하며, 1:1의 중량비로 혼합하여 추출하는 것이 특히 바람직하다.It is preferable to extract the maemaehwa and broiler chickens by mixing them in a weight ratio of 1:2 to 2:1, and it is particularly preferable to extract them by mixing them in a weight ratio of 1:1.
추출시 추출용매로는 70% 에탄올을 사용하는 것이 바람직하다. 추출방법으로는 추출에 통상적으로 사용되는 방법을 사용할 수 있다. It is preferable to use 70% ethanol as an extraction solvent during extraction. As the extraction method, a method commonly used for extraction may be used.
상기 매괴화와 육계의 혼합추출물은, 매괴화와 육계의 혼합물을 8~12배(v/w)의 70% 에탄올에 넣어 55~65℃에서 20~28시간 동안 침지시키고, 침지 후 추출액을 수득하고, 수득한 추출액을 여과하고, 여과된 추출액을 감압농축 및 건조시켜 제조하는 것이 보다 바람직하다. The mixed extract of maemaekhwa and broiler was put in 8-12 times (v/w) 70% ethanol and immersed at 55-65℃ for 20-28 hours, and the extract was obtained after immersion. It is more preferable to prepare by filtering the obtained extract and concentrating and drying the filtered extract under reduced pressure.
침지하여 추출액을 얻는 단계는 2~3회 반복할 수 있으며, 반복하는 경우 얻어진 추출액은 함께 모아 이후 여과과정을 거친다.The step of obtaining the extract by immersion can be repeated 2-3 times, and in the case of repeating, the obtained extract is collected together and then subjected to a filtration process.
본 발명의 조성물은 간손상 및 간질환을 개선하는 효과가 우수하므로, 간손상 및 간질환을 개선하기 위한 약학적 조성물, 건강식품 등에 이용될 수 있다. Since the composition of the present invention is excellent in the effect of improving liver damage and liver disease, it can be used as a pharmaceutical composition, health food, etc. for improving liver damage and liver disease.
이하 실시예를 통해 본 발명을 보다 상세하게 설명한다. 이들 실시예는 본 발명을 예시하는 것으로서 본 발명의 범위가 이에 한정되는 것은 아니다.The present invention will be described in more detail with reference to the following examples. These examples illustrate the present invention, but the scope of the present invention is not limited thereto.
<실시예 1><Example 1>
(주)휴먼허브(http://www.humanherb.co.kr/)에서 구입한 육계와 가나안약초(https://www.canaanherb.com)에서 구입한 매괴화를 1:1의 중량비로 혼합한 혼합물 100g을 70% 에탄올 1,000㎖에 60℃에서 24시간 동안 침지시켰다. Broilers purchased from Human Hub Co., Ltd. (http://www.humanherb.co.kr/) and broilers purchased from Canaan Herbs (https://www.canaanherb.com) are mixed in a weight ratio of 1:1. 100 g of one mixture was immersed in 1,000 ml of 70% ethanol at 60° C. for 24 hours.
침지 후 실온에서 추출액을 수득하고, 다시 70% 에탄올 1,000㎖를 가하여 2회 더 추출하여 추출액을 모았다.After immersion, an extract was obtained at room temperature, and 1,000 ml of 70% ethanol was added thereto and extracted twice more to collect the extract.
모은 추출액을 여과하고, 여과한 여과물을 감압 회전농축기(Vaccum rotary evaporator; 일본 Nihon Seiko사, VR-205c)로 용매를 증발시키는 감압 농축 및 건조과정을 통하여 70% 에탄올 추출물을 얻었다.The collected extract was filtered, and the filtered filtrate was concentrated under reduced pressure to evaporate the solvent with a vacuum rotary evaporator (Nihon Seiko, Japan, VR-205c) and dried to obtain a 70% ethanol extract.
<실시예 2><Example 2>
매괴화와 육계를 1:2의 중량비로 혼합한 혼합물 100g을 원료로 사용한 것을 제외하고는 실시예 1과 동일한 방법으로 70% 에탄올 추출물을 얻었다.A 70% ethanol extract was obtained in the same manner as in Example 1, except that 100 g of a mixture of maemaekhwa and broiler chicken in a weight ratio of 1:2 was used as a raw material.
<실시예 3><Example 3>
매괴화와 육계를 2:1의 중량비로 혼합한 혼합물 100g을 원료로 사용한 것을 제외하고는 실시예 1과 동일한 방법으로 70% 에탄올 추출물을 얻었다.A 70% ethanol extract was obtained in the same manner as in Example 1, except that 100 g of a mixture of maemaekhwa and broiler chicken in a weight ratio of 2:1 was used as a raw material.
<비교예 1><Comparative Example 1>
매괴화 100g을 원료로 사용한 것을 제외하고는 실시예 1과 동일한 방법으로 70% 에탄올 추출물을 얻었다.A 70% ethanol extract was obtained in the same manner as in Example 1, except that 100 g of maekaehwa was used as a raw material.
<비교예 2><Comparative Example 2>
육계 100g을 원료로 사용한 것을 제외하고는 실시예 1과 동일한 방법으로 70% 에탄올 추출물을 얻었다.A 70% ethanol extract was obtained in the same manner as in Example 1, except that 100 g of broiler chicken was used as a raw material.
<비교예 3><Comparative Example 3>
추출용매로 열수를 사용한 것을 제외하고는 실시예 1과 동일한 방법으로 열수 추출물을 얻었다.A hot water extract was obtained in the same manner as in Example 1, except that hot water was used as the extraction solvent.
<실험예 1><Experimental Example 1>
급성 알코올 투여에 의한 간손상 유도 모델에서 매괴화와 육계의 혼합추출물의 효과Effect of mixed extracts of maekgae and broiler in a model of induction of liver damage by acute alcohol administration
실험동물은 Sprague-Dawley 래트(rat) 7주령 수컷을 사용하였다. As the experimental animal, a 7-week-old male Sprague-Dawley rat was used.
실험시료로는 매괴화와 육계의 1:1 혼합추출물(실시예 1), 매괴화와 육계의 1:2 혼합추출물(실시예 2), 매괴화와 육계의 2:1 혼합추출물(실시예 3), 매괴화 단독 추출물(비교예 1) 및 육계 단독 추출물(비교예 2)을 생리식염수에 용해시켜 사용하였으며, 200mg/kg 농도로 0.5㎖씩 경구투여하였다. 양성대조군(PC)에는 생리식염수를 동량 경구투여하였다.As experimental samples, 1:1 mixed extract of maemaekhwa and broiler (Example 1), 1:2 mixed extract of maemaekhwa and broiler (Example 2), and 2:1 mixed extract of maekaehwa and broiler (Example 3) ), a single extract of maegaehwa (Comparative Example 1) and a single extract of broiler chicken (Comparative Example 2) were dissolved in physiological saline and administered orally at a concentration of 200 mg/kg by 0.5 ml each. The same amount of physiological saline was orally administered to the positive control group (PC).
실험시료를 투여하고 30분 후 체중 kg당 4g의 50% 에탄올을 경구투여하여 급성 알코올 투여에 의한 간손상을 유도하여 급성 알코올 투여에 의한 간손상 유도 모델(in vivo)을 얻었다.30 minutes after administration of the experimental sample, 4 g of 50% ethanol per kg of body weight was orally administered to induce liver damage by acute alcohol administration, thereby obtaining a liver injury induction model (in vivo) by acute alcohol administration.
상기 급성 알코올 투여에 의한 간손상 유도 모델에서 에탄올을 경구투여하고 45분 후, 3시간 후 및 8시간 후에 복부대정맥에서 채혈하였다. In the liver injury induction model by the acute alcohol administration, ethanol was orally administered, and blood was collected from the abdominal vena cava after 45 minutes, 3 hours, and 8 hours.
혈액은 실온에서 30분 동안 방치한 후 원심분리(3,000rpm, 10분)하여 혈청만 채취하여 에탄올 키트(ethanol assay kit)를 사용하여 혈중 알코올 농도(ethanol in serum)를 분석하고 아세트알데하이드 탈수소효소 키트(acetaldehyde assay kit)를 이용하여 ALDH(아세트알데하이드 탈수소효소) 활성을 분석하였다. 그 결과를 도 1에 나타내었다.The blood was left at room temperature for 30 minutes, then centrifuged (3,000 rpm, 10 minutes) to collect only the serum, and analyzed the ethanol in serum using an ethanol assay kit, followed by an acetaldehyde dehydrogenase kit. (acetaldehyde assay kit) was used to analyze ALDH (acetaldehyde dehydrogenase) activity. The results are shown in FIG. 1 .
도 1에서와 같이, 급성 알코올 투여에 의한 간손상 유도 모델에서, 에탄올 투여군은 높은 혈중 알코올 농도를 나타내었고, 매괴화와 육계의 1:1 혼합추출물을 투여한 실험군은 매괴화 단독 추출물과 육계 단독 추출물을 투여한 실험군에 비해 혈중 알코올 농도가 현저히 낮은 것으로 확인되었다. 또한 ALDH 활성도 매괴화와 육계의 1:1 혼합추출물을 투여한 실험군에서 가장 높은 것으로 확인되었다.As shown in FIG. 1 , in the liver injury induction model by acute alcohol administration, the ethanol administration group showed a high blood alcohol concentration, and the experimental group administered with a 1:1 mixed extract of maechuhwa and broiler alone extract and broiler alone It was confirmed that the blood alcohol concentration was significantly lower than that of the experimental group to which the extract was administered. Also, it was confirmed that the ALDH activity was the highest in the experimental group administered with a 1:1 mixed extract of maemohwa and broiler.
<실험예 2><Experimental Example 2>
급성 알코올 투여에 의한 간손상 유도 모델에서 추출용매에 따른 효과Effect of Extraction Solvent in Acute Alcohol Administration-induced Liver Injury Model
실험시료로 매괴화와 육계의 70% 에탄올 추출물(실시예 1)과 매괴화와 육계의 열수추출물(비교예 3)을 사용한 것을 제외하고는 실험예 1과 동일한 방법으로 실험하였다.Experiments were carried out in the same manner as in Experimental Example 1, except that a 70% ethanol extract of maekaehwa and broiler chickens (Example 1) and a hot water extract of maebaehwa and broiler chickens (Comparative Example 3) were used as experimental samples.
그 결과를 나타낸 도 1에서와 같이, 70% 에탄올을 추출용매로 사용한 추출물이 열수추출물에 비해 혈중 알코올 농도와 아세트알데하이드 탈수소효소 활성에서 모두 매우 우수한 효과를 나타내었다.As shown in FIG. 1 showing the results, the extract using 70% ethanol as an extraction solvent showed very good effects in both blood alcohol concentration and acetaldehyde dehydrogenase activity compared to the hot water extract.
<실험예 3><Experimental Example 3>
만성 알코올 투여에 의한 간손상 유도 모델에서 매괴화와 육계의 혼합추출물의 효과Effect of a mixed extract of maekgaehwa and broiler in a model of induction of liver damage by chronic alcohol
실험동물은 Sprague-Dawley 래트 7주령 수컷을 사용하였다. As the experimental animal, a 7-week-old male Sprague-Dawley rat was used.
실험시료로는 매괴화와 육계의 1:1 혼합추출물(실시예 1), 매괴화 단독 추출물(비교예 1) 및 육계 단독 추출물(비교예 2)을 생리식염수에 용해시켜 사용하였으며, 200mg/kg 농도로 0.5㎖씩 경구투여하였다. 양성대조군(PC)에는 생리식염수를 동량 경구투여하였다.As an experimental sample, a 1:1 mixed extract of maekaehwa and broiler chickens (Example 1), a single extract of maegaehwa (Comparative Example 1) and a single broiler extract (Comparative Example 2) were dissolved in physiological saline and used, 200mg/kg It was orally administered at a concentration of 0.5 ml each. The same amount of physiological saline was orally administered to the positive control group (PC).
실험시료를 투여하고 30분 후 체중 kg당 4g의 30% 에탄올을 8주 동안 경구투여하여 만성 알코올 투여에 의한 간손상을 유도하여 만성 알코올 투여에 의한 간손상 유도 모델(in vivo)을 얻었다. 양성대조군(PC)에는 생리식염수를 동일한 방법으로 공급하였다. 동물 실험실의 사육조건은 온도 23±2℃, 습도 55±5%를 유지하고 물과 식이는 자유공급하였다. 30 minutes after administration of the experimental sample, 4 g of 30% ethanol per kg of body weight was orally administered for 8 weeks to induce liver damage by chronic alcohol administration, thereby obtaining a liver injury induction model (in vivo) by chronic alcohol administration. The positive control group (PC) was supplied with physiological saline in the same manner. The breeding conditions of the animal laboratory were maintained at 23±2℃ and 55±5% humidity, and water and food were freely supplied.
실험동물을 해부하기 전 24시간 동안 절식시키고 럼푼과 졸레틸을 1:2의 비율로 혼합한 마취제로 마취한 후 개복하여 복부대정맥에서 혈액을 채취하고, 간 조직은 적출하여 무게를 측정하고 조직의 구조학적 형태변화를 분석하였다. 그 결과를 도 2에 나타내었다.Animals were fasted for 24 hours before dissection, anesthetized with an anesthetic mixed with rumpun and zoletyl in a ratio of 1:2, and then opened open to collect blood from the abdominal vena cava. Structural morphological changes were analyzed. The results are shown in FIG. 2 .
도 2에서와 같이, 만성 알코올 투여에 의한 간손상 유도 모델(in vivo )에서 적출한 간 조직의 변화를 확인한 결과, 에탄올 투여군에서 중심정맥(central vein) 주위의 염증, 간세포 괴사와 비대 및 소포성 지방변성이 나타나 있었지만 매괴화와 육계의 혼합추출물에서는 에탄올 투여군에 비해 간세포 사이의 염증과 간세포 괴사와 비대 및 소포성 지방변성이 급격하게 줄어드는 효과를 나타내었다.As shown in Figure 2, as a result of confirming the changes in the liver tissue extracted from the liver injury induction model (in vivo ) by chronic alcohol administration, inflammation around the central vein, hepatocellular necrosis and hypertrophy and vesicles in the ethanol administration group Although fatty degeneration was observed, the mixed extract of broiler broiler and broiler showed the effect of drastically reducing inflammation between hepatocytes, hepatocellular necrosis, hypertrophy, and vesicular steato-degeneration compared to the ethanol-treated group.
또한 각 추출물의 간보호기능을 확인하기 위하여, 알코올 투여 후 시간대별로 채취한 혈청을 생화학 측정기 BS-220(Mindray, New York, NY, USA)을 사용하여 간기능 지표성분을 측정하였다. 간기능 지표성분으로는 혈장의 생화학적 지표인 GOP(aspartic acid transaminase, AST), GPT(alanine transaminase, ALT) 및 ALP(alkaline phosphatase) 활성을 측정하고, 알코올에 의한 영양 손실의 평가를 위한 총 단백질(total-protein), ALB(albumin)와 신장 기능상태를 확인하는 크레아틴(creatinine)을 측정하였다. 알코올 투여 후 시간대별로 채취한 혈청은 생화학 측정기 BS-220 (Mindray, New York, NY, USA)로 상기 생화학적 성분을 분석하였으며, 그 결과를 도 2에 나타내었다. In addition, in order to confirm the hepatoprotective function of each extract, the liver function indicator components were measured using a biochemical measuring instrument BS-220 (Mindray, New York, NY, USA) for serum collected by time after alcohol administration. As liver function indicator components, plasma biochemical indicators of GOP (aspartic acid transaminase, AST), GPT (alanine transaminase, ALT) and ALP (alkaline phosphatase) activity are measured, and total protein for evaluation of nutrient loss due to alcohol. (total-protein), ALB (albumin), and creatine (creatinine) to check the renal function were measured. Serum collected by time after alcohol administration was analyzed for the biochemical components with a biochemical measuring instrument BS-220 (Mindray, New York, NY, USA), and the results are shown in FIG. 2 .
도 2에서와 같이, 만성 알코올섭취에 따른 간기능 지표인 혈장 GOT, GPT, ALP, ALB, 총 단백질, 크레아틴 활성을 분석한 결과, 에탄올 투여군은 정상군에 비해 GOT, GPT, ALP가 약 58%, 61%, 47% 증가하였다. 그러나, 매괴화와 육계의 혼합추출물을 투여한 실험군에서는 혈장 GOT, GPT, ALP, ALB, 총 단백질, 크레아틴 활성 모두 에탄올 투여군에 비해 우수한 간기능 개선 효과를 나타내었다.As shown in FIG. 2, as a result of analyzing plasma GOT, GPT, ALP, ALB, total protein, and creatine activity, which are indicators of liver function according to chronic alcohol intake, the ethanol administration group showed about 58% of GOT, GPT, and ALP compared to the normal group. , increased by 61% and 47%. However, in the experimental group administered with the mixed extract of mae maekhwa and broiler chicken, plasma GOT, GPT, ALP, ALB, total protein, and creatine activity all showed superior liver function improvement effects compared to the ethanol-administered group.
<실험예 4><Experimental Example 4>
비알코올성 지질 축적에 대한 효과Effect on nonalcoholic lipid accumulation
비알코올성 지질 축적 효과를 비교하기 위하여, HepG2 세포에서의 중성지방 생성량을 세포질 내 지방(intracytoplasmic lipids) 측정에 적합한 Oil Red O 염색법으로 확인하였다. In order to compare the effect of non-alcoholic lipid accumulation, the amount of triglyceride in HepG2 cells was confirmed by Oil Red O staining suitable for measuring intracytoplasmic lipids.
실험시료로 매괴화와 육계의 1:1 혼합추출물(실시예 1), 매괴화 단독 추출물(비교예 1) 및 육계 단독 추출물(비교예 2)을 각각 0, 12.5㎍/㎖, 25㎍/㎖, 50㎍/㎖, 100㎍/㎖의 농도로 사용하였다.As experimental samples, a 1:1 mixed extract of maekaehwa and broiler chickens (Example 1), a single extract of maebaehwa (Comparative Example 1), and a single broiler extract (Comparative Example 2) were respectively 0, 12.5㎍/㎖, and 25㎍/㎖ , were used at concentrations of 50 μg/ml and 100 μg/ml.
6-웰 플레이트에서 1.5×105 cell/well의 HepG-2 세포를 24시간 동안 안정화시켰다. 안정화된 HepG2 세포에 시료를 농도별로 처리하고 1시간 후 200μM 팔미트산으로 36시간 동안 지방증을 유도하였다. 36시간이 지난 후 PBS로 2회 세척하고 10% 포르말린으로 30분 동안 고정하였으며, 40% 이소프로판올과 증류수로 세척하는 과정까지 거친 뒤 플레이트를 완전히 건조시켜 이소프로판올에 용해시킨 Oil Red O 염색약을 처리하였다. 1시간 동안 진탕기에서 염색한 다음 증류수를 이용하여 수회 세척하였다. 세척 후 역상 현미경으로 관찰하였고, 그 결과를 도 3에 나타내었다.In a 6-well plate, 1.5×10 5 cells/well of HepG-2 cells were stabilized for 24 hours. Stabilized HepG2 cells were treated with samples by concentration, and after 1 hour, steatosis was induced with 200 μM palmitic acid for 36 hours. After 36 hours, it was washed twice with PBS, fixed with 10% formalin for 30 minutes, washed with 40% isopropanol and distilled water, and then the plate was completely dried and treated with Oil Red O dye dissolved in isopropanol. After dyeing on a shaker for 1 hour, it was washed several times with distilled water. After washing, it was observed with an inverted microscope, and the results are shown in FIG. 3 .
도 3의 결과에서와 같이, 팔미트산을 이용하여 중성지방 생성량을 확인한 결과, 매괴화와 육계의 혼합추출물을 투여한 실험군이 매괴화 단독 추출물과 육계 단독 추출물을 투여한 실험군에 비해 중성지방의 축적이 감소한 것을 확인하였다. 특히 100㎍/㎖ 농도에서 지방 축적이 약 66% 감소한 것을 확인하였다.As shown in the results of FIG. 3 , as a result of checking the amount of triglyceride production using palmitic acid, the experimental group administered with the mixed extract of mae mae hwa and broiler had less triglyceride than the experimental group administered with the mae mae hwa and broiler extract alone. It was confirmed that the accumulation was reduced. In particular, it was confirmed that the fat accumulation was reduced by about 66% at the concentration of 100 μg/ml.
<실험예 5><Experimental Example 5>
비알코올성 염증에 대한 효과Effects on non-alcoholic inflammation
본 발명의 매괴화와 육계의 혼합추출물이 비알코올성 염증에 미치는 효과를 확인하기 위하여 실험하였다.An experiment was conducted to confirm the effect of the mixed extract of the present invention on non-alcoholic inflammation.
실험시료로 매괴화와 육계의 1:1 혼합추출물(실시예 1), 매괴화 단독 추출물(비교예 1) 및 육계 단독 추출물(비교예 2)을 각각 0, 12.5㎍/㎖, 25㎍/㎖, 50㎍/㎖, 100㎍/㎖의 농도로 사용하였다.As experimental samples, a 1:1 mixed extract of maekaehwa and broiler chickens (Example 1), a single extract of maebaehwa (Comparative Example 1), and a single broiler extract (Comparative Example 2) were respectively 0, 12.5㎍/㎖, and 25㎍/㎖ , were used at concentrations of 50 μg/ml and 100 μg/ml.
(1) 세포생존율(MTS assay)(1) Cell viability (MTS assay)
HepG-2 세포의 생존율을 미토콘드리아 디하이드로게나제(Mitochondrial Dehydrogenases)에 의하여 MTS가 포르마잔(Formazan)으로 전환되는 것을 측정하는 MTS 분석법(MTS Assay)으로 확인하였다.The viability of HepG-2 cells was confirmed by the MTS assay measuring the conversion of MTS to formazan by mitochondrial dehydrogenases (MTS Assay).
96-웰 플레이트에 웰 당 1.5×105 cell/well의 HepG-2 세포를 분주하고 24시간 동안 안정화시켰다. 안정화된 HepG-2 세포에 각 시료를 농도별로 처리하고 24시간 동안 배양하였다. 20㎕의 MTS 용액을 첨가하여 CO2 배양기(37℃, 5% CO2)에서 1시간 동안 반응시킨 후 450nm에서 흡광도를 측정하여 대조군에 대한 세포생존율을 백분율로 표시하였다.In a 96-well plate, 1.5×10 5 cells/well of HepG-2 cells per well were seeded and stabilized for 24 hours. Stabilized HepG-2 cells were treated with each sample by concentration and cultured for 24 hours. After 20 μl of MTS solution was added and reacted in a CO 2 incubator (37° C., 5% CO 2 ) for 1 hour, the absorbance was measured at 450 nm to express the cell viability as a percentage for the control.
(2) 아질산염(Nitrite) 생성량(NO assay)(2) Nitrite production (NO assay)
아질산염 생성량은 Green 등의 방법에 따라 확인하였다.The amount of nitrite production was confirmed according to the method of Green et al.
96-웰 플레이트에 웰 당 1.5×105 cell/well의 HepG-2 세포를 분주하고 24시간 동안 안정화시켰다. 안정화된 HepG-2 세포에 각 시료를 농도별로 처리하고 1시간 후 LPS(lipopolysaccharide, 100ng/㎖)를 처리하여 18시간 동안 배양하였다. 배양 후 상층액을 수거하여 상층액과 동량의 그리스 시약(griess reagent)을 첨가하고 실온에서 10분 동안 반응시켜 산화생성물인 NO 생성 정도를 570nm 파장에서 측정하였다.In a 96-well plate, 1.5×10 5 cells/well of HepG-2 cells per well were seeded and stabilized for 24 hours. Stabilized HepG-2 cells were treated with each sample by concentration, and 1 hour later, LPS (lipopolysaccharide, 100 ng/ml) was treated and incubated for 18 hours. After incubation, the supernatant was collected, the same amount of grease reagent was added to the supernatant, and reacted at room temperature for 10 minutes to measure the degree of NO production, which is an oxidation product, at a wavelength of 570 nm.
(3) iNOS / COX-2(3) iNOS / COX-2
96-웰 플레이트에 웰 당 1.5×105 cell/well의 HepG-2 세포를 분주하고 24시간 동안 안정화시켰다. 안정화된 HepG-2 세포에 각 시료를 농도별로 처리하고 1시간 후 LPS(lipopolysaccharide, 100ng/㎖)를 처리하여 18시간 동안 배양하였다. 배양 후 웨스턴블롯 분석법(Western blot analysis)으로 iNOS와 COX-2 단백질 발현을 확인하였다.In a 96-well plate, 1.5×10 5 cells/well of HepG-2 cells per well were seeded and stabilized for 24 hours. Stabilized HepG-2 cells were treated with each sample by concentration, and 1 hour later, LPS (lipopolysaccharide, 100 ng/ml) was treated and incubated for 18 hours. After incubation, the expression of iNOS and COX-2 proteins was confirmed by Western blot analysis.
(4) NF-κB (4) NF-κB
96-웰 플레이트에 웰 당 1.5×105 cell/well의 HepG-2 세포를 분주하고 24시간 동안 안정화시켰다. 안정화된 HepG-2 세포에 각 시료를 농도별로 처리하고 1시간 후 IL-1β(10ng/㎖)로 30분 동안 자극한 후 핵단백질을 분리하여 웨스턴블롯 분석법(Western blot analysis)으로 NF-κB 단백질 발현을 확인하였다.In a 96-well plate, 1.5×10 5 cells/well of HepG-2 cells per well were seeded and stabilized for 24 hours. Each sample was treated in the stabilized HepG-2 cells by concentration and after 1 hour stimulation with IL-1β (10ng/ml) for 30 minutes, nuclear protein was isolated and NF-κB protein was performed by Western blot analysis. Expression was confirmed.
(5) 결과(5) Results
상기와 같이 비알코올성 염증에 미치는 효과를 확인한 결과를 도 4에 나타내었다.The results of confirming the effect on non-alcoholic inflammation as described above are shown in FIG. 4 .
도 4에서와 같이, LPS 자극에 의하여 증가된 NO의 분비량은 매괴화와 육계의 혼합추출물을 전처리하였을 때 농도의존적으로 감소하였다. 또한 염증생성에 관여하는 신호전달인자인 iNOS와 COX-2 및 NF-κB의 발현에 미치는 매괴화와 육계의 혼합추출물의 영향을 확인한 결과, LPS 자극에 의하여 증가된 유전자의 발현이 매괴화와 육계의 혼합추출물의 전처리에 의하여 농도의존적으로 감소하였다.As shown in FIG. 4, the secretion of NO increased by LPS stimulation was decreased in a concentration-dependent manner when the mixed extract of maemaekhwa and broiler was pretreated. In addition, as a result of confirming the effect of the mixed extract of maemon and broiler on the expression of iNOS, COX-2, and NF-κB, which are signaling factors involved in the production of inflammation, the expression of genes increased by LPS stimulation showed that the expression of the gene increased by the broiler and broiler. was decreased in a concentration-dependent manner by pretreatment of the mixed extract of
<실험예 6><Experimental Example 6>
고지방식이 투여에 의한 만성 간손상 유도 모델에서In a chronic liver injury induction model by administration of a high-fat diet 간 및 지방조직의 병리학적인 형태변화에 미치는 효과 Effect on pathological morphological changes in liver and adipose tissue
실험동물은 Sprague-Dawley 래트 7주령 수컷을 사용하였다. As the experimental animal, a 7-week-old male Sprague-Dawley rat was used.
실험시료로는 매괴화와 육계의 1:1 혼합추출물(실시예 1)을 생리식염수에 용해시켜 사용하였으며, 200mg/kg 농도로 0.5㎖씩 경구투여하였다. 양성대조군(PC)에는 생리식염수를 동량 경구투여하였다. As an experimental sample, a 1:1 mixed extract (Example 1) of maebaehwa and broiler chicken was dissolved in physiological saline, and 0.5 ml of each was orally administered at a concentration of 200 mg/kg. The same amount of physiological saline was orally administered to the positive control group (PC).
정상군인 양성 대조군(PC)에는 정상 사료를 공급하고, 실험군인 고지방식이 유도군에는 고지방식 사료를 공급하였다. 동물 실험실의 사육조건은 온도 23±2℃, 습도 55±5%를 유지하고 물과 식이는 자유공급하였다. 매주 1회씩 고지방식 사료 섭취량을 측정하였다. A normal diet was supplied to the positive control group (PC), which is the normal group, and a high-fat diet was supplied to the high-fat diet induction group, which is the experimental group. The breeding conditions of the animal laboratory were maintained at 23±2℃ and 55±5% humidity, and water and food were freely supplied. High-fat diet intake was measured once a week.
실험동물을 해부하기 전 24시간 동안 절식시키고 럼푼과 졸레틸을 1:2의 비율로 혼합한 마취제로 마취한 후 개복하여 복부대정맥에서 혈액을 채취하였다. 또한 간 및 부고환 지방조직을 적출하여 무게를 측정하고, 적출된 간 및 부고환 지방조직은 10% 포르말린에 고정하여 자동조직처리기(automatic tissue processor)에서 탈수, 청명, 파라핀 침투 과정을 거쳐 파라핀 포매 후 4㎛의 조직절편을 자르고 탈파라핀, 함수과정을 거쳐 헤마톡실린-에오신(Harry's hematoxylin-eosin) 염색을 통해 간 및 부고환 지방조직의 구조학적인 손상도 및 형태변화를 확인하였다. 그 결과를 도 5에 나타내었다.Animals were fasted for 24 hours before dissection, anesthetized with an anesthetic mixed with rumpun and zoletyl in a ratio of 1:2, and blood was collected from the abdominal vena cava after laparotomy. In addition, liver and epididymal adipose tissue were excised and weight was measured, and the extracted liver and epididymal adipose tissue was fixed in 10% formalin, dehydrated, cleared and paraffin infiltrated in an automatic tissue processor, followed by paraffin embedding 4 Structural damage and morphological changes of liver and epididymal adipose tissue were confirmed through hematoxylin-eosin staining after cutting a ㎛ tissue section, deparaffinizing, hydrous and hematoxylin-eosin staining. The results are shown in FIG. 5 .
고지방식이를 섭취한 만성(6주) 비알코올성 간손상 모델에서 적출한 간 및 부고환 지방의 병리조직학적 변화를 확인한 결과, 대조군에서는 간세포의 괴사 및 비대가 발생하였고 간조직에 전체적으로 지방세포가 균일하게 배열되어 있었으나 매괴화와 육계의 혼합추출물을 처리한 실험군에서는 간세포의 손상 및 지방세포의 크기와 수가 현저하게 감소하였다. 또한 부고환조직에서도 대조군에 비해 매괴화와 육계의 혼합추출물을 처리한 실험군에서 지방세포의 크기와 수가 현저하게 감소하였다. As a result of confirming histopathological changes in liver and epididymal fat extracted from chronic (6 weeks) non-alcoholic liver injury model ingesting a high-fat diet, hepatocyte necrosis and hypertrophy occurred in the control group, and fat cells were uniform throughout the liver tissue. However, in the experimental group treated with the mixed extract of maekgaehwa and broiler chicken, damage to hepatocytes and the size and number of adipocytes were significantly reduced. Also, in the epididymal tissue, the size and number of adipocytes were significantly reduced in the experimental group treated with the mixed extract of broiler and broiler compared to the control group.
Claims (4)
상기 간손상은 급성 알코올성 간손상, 만성 알코올성 간손상, 비알코올성 지질축적, 비알코올성 염증 및 비알코올성 간손상 중에서 선택된 어느 하나인 것을 특징으로 하는 간손상 개선용 조성물.Liver damage containing a mixed extract of maechuhwa and broiler ( shell of Cinnamomum cassia PRESL) in a weight ratio of 1:2 to 2:1 and extracted with ethanol at a concentration of 25-100㎍/㎖ As an improvement composition,
The liver damage is acute alcoholic liver damage, chronic alcoholic liver damage, non-alcoholic lipid accumulation, non-alcoholic inflammation and non-alcoholic liver damage composition for improving liver damage, characterized in that any one selected from.
상기 에탄올은 70중량% 에탄올인 것을 특징으로 하는 조성물.According to claim 1,
The ethanol is a composition, characterized in that 70% by weight ethanol.
상기 매괴화와 육계의 혼합추출물은,
매괴화와 육계의 혼합물을 8~12배(v/w)의 70중량% 에탄올에 넣어 55~65℃에서 20~28시간 동안 침지시키는 단계;
침지 후 추출액을 수득하는 단계;
수득한 추출액을 여과하는 단계; 및
여과된 추출액을 감압농축 및 건조시키는 단계를 포함하는 방법에 의하여 제조된 것임을 특징으로 하는 조성물.
According to claim 1,
The mixed extract of maegaehwa and broiler chicken,
Putting a mixture of maemaekhwa and broilers in 8-12 times (v/w) 70% by weight ethanol and immersing them at 55-65° C. for 20-28 hours;
obtaining an extract after immersion;
filtering the obtained extract; and
A composition, characterized in that it is prepared by a method comprising the step of concentrating and drying the filtered extract under reduced pressure.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020190154616A KR102266729B1 (en) | 2019-11-27 | 2019-11-27 | Composition for improving liver injury and liver disease comprising flower of Rosa rugosa Thunberg and Cinnamomum cassia PRESL |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020190154616A KR102266729B1 (en) | 2019-11-27 | 2019-11-27 | Composition for improving liver injury and liver disease comprising flower of Rosa rugosa Thunberg and Cinnamomum cassia PRESL |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| KR20210065633A KR20210065633A (en) | 2021-06-04 |
| KR102266729B1 true KR102266729B1 (en) | 2021-06-17 |
Family
ID=76391709
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020190154616A KR102266729B1 (en) | 2019-11-27 | 2019-11-27 | Composition for improving liver injury and liver disease comprising flower of Rosa rugosa Thunberg and Cinnamomum cassia PRESL |
Country Status (1)
| Country | Link |
|---|---|
| KR (1) | KR102266729B1 (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115887542A (en) * | 2022-11-22 | 2023-04-04 | 中南民族大学 | Application of rose ethanol extract in preparation of anti-hepatic fibrosis medicine or health food |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102043813B1 (en) | 2018-12-26 | 2019-11-12 | 이창원 | Composition for liver protection against alcoholic liver damage |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20040087099A (en) | 2003-04-04 | 2004-10-13 | 박재상 | Herb-medical mixture of katzenjammer cure and digester |
| KR100968674B1 (en) | 2008-02-04 | 2010-07-06 | 강홍구 | Herbal composition for alcoholic hangover |
| KR101046787B1 (en) | 2009-02-18 | 2011-07-05 | 황동철 | Hangover drink composition |
| KR101539864B1 (en) | 2013-01-30 | 2015-07-28 | 이항종 | Beverage for curing of hangover |
| KR101490211B1 (en) | 2013-11-15 | 2015-02-06 | 농업회사법인황칠다담주식회사 | Beverage for reducing alcoholic hangup and digester and method for preparation thereof |
| KR20150063905A (en) | 2013-11-25 | 2015-06-10 | 전라남도 | Compositions for preventing or improving of alcoholic liver disease, or reducing alcoholic hangup comprising Rosa rugosa extracts |
| KR101691205B1 (en) * | 2014-03-07 | 2016-12-30 | 동의대학교 산학협력단 | Composition comprising herbal extract for preventing or treating fatty liver disease |
| KR20160085149A (en) * | 2015-01-07 | 2016-07-15 | 대구한의대학교산학협력단 | Composition comprising the extract of Rosa rugosa Thunb for preventing or treating liver disease |
| KR101870280B1 (en) | 2016-06-29 | 2018-06-22 | 고려인삼제조 주식회사 | A composition for removing hangover comprising an extract of Cinnamomum cassia Presl and cinnamomi petiole |
| KR20170023405A (en) * | 2017-02-20 | 2017-03-03 | 대구한의대학교산학협력단 | Composition comprising the extract of Rosa rugosa Thunb for preventing or treating liver disease |
| KR101930277B1 (en) | 2017-07-14 | 2018-12-18 | 코스맥스 주식회사 | Composition for Preventing and Improving Hangover |
| KR101898411B1 (en) * | 2018-03-20 | 2018-09-12 | 대구한의대학교산학협력단 | Composition comprising the extract of Rosa rugosa Thunb for preventing or treating liver disease |
-
2019
- 2019-11-27 KR KR1020190154616A patent/KR102266729B1/en active IP Right Grant
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102043813B1 (en) | 2018-12-26 | 2019-11-12 | 이창원 | Composition for liver protection against alcoholic liver damage |
Non-Patent Citations (2)
| Title |
|---|
| Protective Effects of Cinnamomum cassia Blume in the Fibrogenesis of Activated HSC-T6 Cells and Dimethylnitrosamine-Induced Acute Liver Injury in SD Rats, Bioscience, Biotechnology, and Biochemistry, 74(3), pp.477-483(2014.05.22.) 1부.* |
| 대한한방내과 학회지 제34권 4호(2013.12) 375-383 |
Also Published As
| Publication number | Publication date |
|---|---|
| KR20210065633A (en) | 2021-06-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Akindahunsi et al. | Toxicological investigation of aqueous-methanolic extract of the calyces of Hibiscus sabdariffa L. | |
| Jung et al. | Antihyperglycemic activity of herb extracts on streptozotocin-induced diabetic rats | |
| KR102266729B1 (en) | Composition for improving liver injury and liver disease comprising flower of Rosa rugosa Thunberg and Cinnamomum cassia PRESL | |
| Karau et al. | Efficacy and safety assessment of Launaea cornuta extracts in the management of diabetes mellitus | |
| KR20150051597A (en) | Food Composition for improving liver function containing extract of Arctiumlappa L. | |
| AU2018101556A4 (en) | A kind of nitraria tangutorum bobr seed extract | |
| Nnamonu et al. | Effects of Hibiscus Sabdariffa calyces aqueous extract on serum cholesterol, body weight and liver biomarkers of Rattus Novergicus | |
| KR102402228B1 (en) | Composition for improving hangover cure and alcoholic liver injury comprising pericarp of Areca catechu L and and Ledebouriella seseloides | |
| KR101887764B1 (en) | Process for Extracting of red bean shell having anti obesity and Compositions comprising the Extracts as an active ingredient | |
| KR102309913B1 (en) | Composition for improving hangover cure and alcoholic liver injury comprising root extract of Rosa multiflora THUNB | |
| Tulsawani et al. | Supplementation of fruit extract of Hippophae rhamnoides speeds adaptation to simulated high altitude stressors in rats | |
| KR20100039534A (en) | The processing method of laminaria extracts with chlorophyll | |
| KR101793927B1 (en) | Clerodendron trichotomum Thunberg leaf tea and process thereof | |
| CN113662975A (en) | Chenopodium quinoa willd extract and application thereof in preparation of medicines and foods for preventing gastric mucosal injury | |
| KR20100001635A (en) | Extracts of enteromorpha prolifera having anti-diabetic function, functional foods including the extracts and use of enteromorpha prolifera | |
| Dasofunjo et al. | Effect ofthe ethanolic extract of Piliostigma thonningii leaves on kidney function indices and haematological parameters of male albino wistar rats | |
| Onyegeme-Okerenta et al. | Investigation of the effect of aqueous leaf extract of Senna alata (l) roxb on palm oil-induced hyper-lipidaemic plasma lipid in wister rats | |
| KR102136484B1 (en) | Composition for preventing or treating liver injury and method for preparing the same | |
| KR100655593B1 (en) | Composition for Liver Protection and Reduction of Serum Ethanol Level | |
| Musa et al. | Chronic (52-week) oral toxicity study of herbal tea of Moringa stenopetala and Mentha spicata leaves formulation in Wistar albino rats | |
| البسيوني et al. | Impact of leaves and flower from Alfalfa and Dandelion to Improve Liver Function and preparing Some Food Products | |
| Ibrahim et al. | Biochemical and histological studies on the Ameliorative role of fenugreek products in tannic acid-induced iron deficiency anemia in Albino rats | |
| Abdelgawad et al. | Rams (Haloxylon salicornicum) Powder and Extract in Diets of Male Albino Rats to Cope Hyperglycemia | |
| Ahmad et al. | Development and characterization of locally prepared Aloe Vera juice and its functional potential against Hyperlipidemia. | |
| YounisKhalaf et al. | A Physiological and Histopathological Study on the Protective Effect of Aqueous Leaves Extract of Moringaoleifera against Cisplatin Toxicity in Rats |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| E701 | Decision to grant or registration of patent right | ||
| GRNT | Written decision to grant |