KR102247364B1 - Composition for improving respiratory diseases using the extract of Euonymus alatus - Google Patents
Composition for improving respiratory diseases using the extract of Euonymus alatus Download PDFInfo
- Publication number
- KR102247364B1 KR102247364B1 KR1020190109803A KR20190109803A KR102247364B1 KR 102247364 B1 KR102247364 B1 KR 102247364B1 KR 1020190109803 A KR1020190109803 A KR 1020190109803A KR 20190109803 A KR20190109803 A KR 20190109803A KR 102247364 B1 KR102247364 B1 KR 102247364B1
- Authority
- KR
- South Korea
- Prior art keywords
- extract
- composition
- cells
- food
- inhibits
- Prior art date
Links
- 239000000284 extract Substances 0.000 title claims abstract description 52
- 239000000203 mixture Substances 0.000 title claims abstract description 44
- 208000023504 respiratory system disease Diseases 0.000 title abstract description 17
- 241000208368 Euonymus alatus Species 0.000 title description 2
- 235000013305 food Nutrition 0.000 claims description 33
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 21
- 230000036541 health Effects 0.000 claims description 18
- 235000013376 functional food Nutrition 0.000 claims description 16
- 239000004480 active ingredient Substances 0.000 claims description 13
- 239000008194 pharmaceutical composition Substances 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 239000012046 mixed solvent Substances 0.000 claims description 6
- 210000004027 cell Anatomy 0.000 abstract description 31
- 208000006545 Chronic Obstructive Pulmonary Disease Diseases 0.000 abstract description 25
- 102000004127 Cytokines Human genes 0.000 abstract description 16
- 108090000695 Cytokines Proteins 0.000 abstract description 16
- 230000000694 effects Effects 0.000 abstract description 14
- 210000004072 lung Anatomy 0.000 abstract description 14
- 208000019693 Lung disease Diseases 0.000 abstract description 13
- 210000000440 neutrophil Anatomy 0.000 abstract description 12
- 101710151803 Mitochondrial intermediate peptidase 2 Proteins 0.000 abstract description 11
- 230000002401 inhibitory effect Effects 0.000 abstract description 9
- 230000002757 inflammatory effect Effects 0.000 abstract description 7
- 230000004913 activation Effects 0.000 abstract description 6
- 102000019034 Chemokines Human genes 0.000 abstract description 5
- 108010012236 Chemokines Proteins 0.000 abstract description 5
- PHEDXBVPIONUQT-RGYGYFBISA-N phorbol 13-acetate 12-myristate Chemical compound C([C@]1(O)C(=O)C(C)=C[C@H]1[C@@]1(O)[C@H](C)[C@H]2OC(=O)CCCCCCCCCCCCC)C(CO)=C[C@H]1[C@H]1[C@]2(OC(C)=O)C1(C)C PHEDXBVPIONUQT-RGYGYFBISA-N 0.000 abstract description 5
- 210000002540 macrophage Anatomy 0.000 abstract description 4
- 238000010172 mouse model Methods 0.000 abstract description 4
- 210000003097 mucus Anatomy 0.000 abstract description 4
- 210000003979 eosinophil Anatomy 0.000 abstract description 3
- 210000002865 immune cell Anatomy 0.000 abstract description 3
- 210000001132 alveolar macrophage Anatomy 0.000 abstract description 2
- 210000000424 bronchial epithelial cell Anatomy 0.000 abstract description 2
- 230000000451 tissue damage Effects 0.000 abstract description 2
- 231100000827 tissue damage Toxicity 0.000 abstract description 2
- 102000004890 Interleukin-8 Human genes 0.000 abstract 1
- 108090001007 Interleukin-8 Proteins 0.000 abstract 1
- 230000009545 invasion Effects 0.000 abstract 1
- 210000004698 lymphocyte Anatomy 0.000 abstract 1
- 230000028327 secretion Effects 0.000 abstract 1
- -1 etc.) Chemical compound 0.000 description 12
- 235000013373 food additive Nutrition 0.000 description 12
- 239000002778 food additive Substances 0.000 description 12
- 239000003814 drug Substances 0.000 description 11
- 229940079593 drug Drugs 0.000 description 10
- 238000009472 formulation Methods 0.000 description 10
- 235000019441 ethanol Nutrition 0.000 description 8
- 239000002904 solvent Substances 0.000 description 8
- 238000004458 analytical method Methods 0.000 description 7
- 150000001875 compounds Chemical class 0.000 description 7
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 7
- 239000000796 flavoring agent Substances 0.000 description 7
- 230000006872 improvement Effects 0.000 description 7
- 238000000034 method Methods 0.000 description 7
- 239000002953 phosphate buffered saline Substances 0.000 description 7
- 239000000843 powder Substances 0.000 description 7
- 239000006228 supernatant Substances 0.000 description 7
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 6
- 229920002472 Starch Polymers 0.000 description 6
- HQKMJHAJHXVSDF-UHFFFAOYSA-L magnesium stearate Chemical compound [Mg+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O HQKMJHAJHXVSDF-UHFFFAOYSA-L 0.000 description 6
- 238000004519 manufacturing process Methods 0.000 description 6
- 230000004202 respiratory function Effects 0.000 description 6
- 210000001519 tissue Anatomy 0.000 description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 5
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 description 5
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 5
- 239000003795 chemical substances by application Substances 0.000 description 5
- 238000000605 extraction Methods 0.000 description 5
- 235000013355 food flavoring agent Nutrition 0.000 description 5
- 239000004615 ingredient Substances 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- 235000019698 starch Nutrition 0.000 description 5
- 208000024891 symptom Diseases 0.000 description 5
- MZOFCQQQCNRIBI-VMXHOPILSA-N (3s)-4-[[(2s)-1-[[(2s)-1-[[(1s)-1-carboxy-2-hydroxyethyl]amino]-4-methyl-1-oxopentan-2-yl]amino]-5-(diaminomethylideneamino)-1-oxopentan-2-yl]amino]-3-[[2-[[(2s)-2,6-diaminohexanoyl]amino]acetyl]amino]-4-oxobutanoic acid Chemical compound OC[C@@H](C(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@@H](N)CCCCN MZOFCQQQCNRIBI-VMXHOPILSA-N 0.000 description 4
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 4
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 4
- 208000000059 Dyspnea Diseases 0.000 description 4
- 206010013975 Dyspnoeas Diseases 0.000 description 4
- 238000002965 ELISA Methods 0.000 description 4
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 4
- 206010036790 Productive cough Diseases 0.000 description 4
- 244000269722 Thea sinensis Species 0.000 description 4
- 108060008682 Tumor Necrosis Factor Proteins 0.000 description 4
- 102000000852 Tumor Necrosis Factor-alpha Human genes 0.000 description 4
- 239000000428 dust Substances 0.000 description 4
- 230000002708 enhancing effect Effects 0.000 description 4
- 235000003599 food sweetener Nutrition 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 230000000241 respiratory effect Effects 0.000 description 4
- 208000013220 shortness of breath Diseases 0.000 description 4
- 230000000391 smoking effect Effects 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 208000024794 sputum Diseases 0.000 description 4
- 210000003802 sputum Anatomy 0.000 description 4
- 239000008107 starch Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 239000003765 sweetening agent Substances 0.000 description 4
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 description 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 3
- 206010001052 Acute respiratory distress syndrome Diseases 0.000 description 3
- 208000000884 Airway Obstruction Diseases 0.000 description 3
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 3
- 102100023698 C-C motif chemokine 17 Human genes 0.000 description 3
- 102100021943 C-C motif chemokine 2 Human genes 0.000 description 3
- 101710155857 C-C motif chemokine 2 Proteins 0.000 description 3
- 206010011224 Cough Diseases 0.000 description 3
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 3
- 102100031107 Disintegrin and metalloproteinase domain-containing protein 11 Human genes 0.000 description 3
- 101710121366 Disintegrin and metalloproteinase domain-containing protein 11 Proteins 0.000 description 3
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 3
- 108010017213 Granulocyte-Macrophage Colony-Stimulating Factor Proteins 0.000 description 3
- 102100039620 Granulocyte-macrophage colony-stimulating factor Human genes 0.000 description 3
- 241000282412 Homo Species 0.000 description 3
- 101000978362 Homo sapiens C-C motif chemokine 17 Proteins 0.000 description 3
- 108090001005 Interleukin-6 Proteins 0.000 description 3
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 241000699666 Mus <mouse, genus> Species 0.000 description 3
- 102000035195 Peptidases Human genes 0.000 description 3
- 108091005804 Peptidases Proteins 0.000 description 3
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 3
- 229930006000 Sucrose Natural products 0.000 description 3
- 206010069351 acute lung injury Diseases 0.000 description 3
- 235000013361 beverage Nutrition 0.000 description 3
- 210000000621 bronchi Anatomy 0.000 description 3
- 239000011575 calcium Substances 0.000 description 3
- 239000002775 capsule Substances 0.000 description 3
- 239000001768 carboxy methyl cellulose Substances 0.000 description 3
- 229920002678 cellulose Polymers 0.000 description 3
- 235000010980 cellulose Nutrition 0.000 description 3
- 238000005119 centrifugation Methods 0.000 description 3
- 235000009508 confectionery Nutrition 0.000 description 3
- 239000008121 dextrose Substances 0.000 description 3
- 238000002224 dissection Methods 0.000 description 3
- 239000002158 endotoxin Substances 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 239000012530 fluid Substances 0.000 description 3
- 239000007789 gas Substances 0.000 description 3
- 239000000499 gel Substances 0.000 description 3
- 239000008187 granular material Substances 0.000 description 3
- 230000001771 impaired effect Effects 0.000 description 3
- 230000006698 induction Effects 0.000 description 3
- 239000008101 lactose Substances 0.000 description 3
- 229920006008 lipopolysaccharide Polymers 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 235000019359 magnesium stearate Nutrition 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 229920000609 methyl cellulose Polymers 0.000 description 3
- 235000010981 methylcellulose Nutrition 0.000 description 3
- 239000001923 methylcellulose Substances 0.000 description 3
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 3
- 230000008506 pathogenesis Effects 0.000 description 3
- 239000006187 pill Substances 0.000 description 3
- NLKNQRATVPKPDG-UHFFFAOYSA-M potassium iodide Chemical compound [K+].[I-] NLKNQRATVPKPDG-UHFFFAOYSA-M 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 238000010186 staining Methods 0.000 description 3
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 3
- 239000005720 sucrose Substances 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- 239000006188 syrup Substances 0.000 description 3
- 235000020357 syrup Nutrition 0.000 description 3
- 239000003826 tablet Substances 0.000 description 3
- PUPZLCDOIYMWBV-UHFFFAOYSA-N (+/-)-1,3-Butanediol Chemical compound CC(O)CCO PUPZLCDOIYMWBV-UHFFFAOYSA-N 0.000 description 2
- GAKUNXBDVGLOFS-DUZKARGPSA-N (1-acetyloxy-3-hexadecanoyloxypropan-2-yl) (9z,12z)-octadeca-9,12-dienoate Chemical compound CCCCCCCCCCCCCCCC(=O)OCC(COC(C)=O)OC(=O)CCCCCCC\C=C/C\C=C/CCCCC GAKUNXBDVGLOFS-DUZKARGPSA-N 0.000 description 2
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 2
- 235000017491 Bambusa tulda Nutrition 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 2
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 2
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 description 2
- RGHNJXZEOKUKBD-SQOUGZDYSA-N D-gluconic acid Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C(O)=O RGHNJXZEOKUKBD-SQOUGZDYSA-N 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 2
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 2
- VZCYOOQTPOCHFL-OWOJBTEDSA-N Fumaric acid Chemical compound OC(=O)\C=C\C(O)=O VZCYOOQTPOCHFL-OWOJBTEDSA-N 0.000 description 2
- 108010010803 Gelatin Proteins 0.000 description 2
- 208000034826 Genetic Predisposition to Disease Diseases 0.000 description 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- 239000012981 Hank's balanced salt solution Substances 0.000 description 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
- 206010061218 Inflammation Diseases 0.000 description 2
- 102100037850 Interferon gamma Human genes 0.000 description 2
- 108010074328 Interferon-gamma Proteins 0.000 description 2
- 208000029523 Interstitial Lung disease Diseases 0.000 description 2
- 206010022998 Irritability Diseases 0.000 description 2
- 241000124008 Mammalia Species 0.000 description 2
- 229930195725 Mannitol Natural products 0.000 description 2
- 102000003896 Myeloperoxidases Human genes 0.000 description 2
- 108090000235 Myeloperoxidases Proteins 0.000 description 2
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 2
- GAKUNXBDVGLOFS-UHFFFAOYSA-N PLA Natural products CCCCCCCCCCCCCCCC(=O)OCC(COC(C)=O)OC(=O)CCCCCCCC=CCC=CCCCCC GAKUNXBDVGLOFS-UHFFFAOYSA-N 0.000 description 2
- 102000016387 Pancreatic elastase Human genes 0.000 description 2
- 108010067372 Pancreatic elastase Proteins 0.000 description 2
- OFBQJSOFQDEBGM-UHFFFAOYSA-N Pentane Chemical compound CCCCC OFBQJSOFQDEBGM-UHFFFAOYSA-N 0.000 description 2
- 244000082204 Phyllostachys viridis Species 0.000 description 2
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 2
- 239000012980 RPMI-1640 medium Substances 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 240000008042 Zea mays Species 0.000 description 2
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 2
- DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 description 2
- 239000013543 active substance Substances 0.000 description 2
- WNLRTRBMVRJNCN-UHFFFAOYSA-N adipic acid Chemical compound OC(=O)CCCCC(O)=O WNLRTRBMVRJNCN-UHFFFAOYSA-N 0.000 description 2
- 150000001298 alcohols Chemical class 0.000 description 2
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 2
- 230000003266 anti-allergic effect Effects 0.000 description 2
- 230000003110 anti-inflammatory effect Effects 0.000 description 2
- 208000006673 asthma Diseases 0.000 description 2
- 239000011425 bamboo Substances 0.000 description 2
- 239000011230 binding agent Substances 0.000 description 2
- VDPDRYUUTXEEIE-UHFFFAOYSA-N bis(methylsulfonyl)methane Chemical compound CS(=O)(=O)CS(C)(=O)=O VDPDRYUUTXEEIE-UHFFFAOYSA-N 0.000 description 2
- 206010006451 bronchitis Diseases 0.000 description 2
- 229910052791 calcium Inorganic materials 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 239000001569 carbon dioxide Substances 0.000 description 2
- 229910002092 carbon dioxide Inorganic materials 0.000 description 2
- 239000001913 cellulose Substances 0.000 description 2
- 230000001684 chronic effect Effects 0.000 description 2
- 235000005822 corn Nutrition 0.000 description 2
- 230000000875 corresponding effect Effects 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 235000014113 dietary fatty acids Nutrition 0.000 description 2
- 239000003085 diluting agent Substances 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 239000003937 drug carrier Substances 0.000 description 2
- 239000003995 emulsifying agent Substances 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 239000000194 fatty acid Substances 0.000 description 2
- 229930195729 fatty acid Natural products 0.000 description 2
- 235000019634 flavors Nutrition 0.000 description 2
- 239000008273 gelatin Substances 0.000 description 2
- 229920000159 gelatin Polymers 0.000 description 2
- 235000019322 gelatine Nutrition 0.000 description 2
- 235000011852 gelatine desserts Nutrition 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 235000009569 green tea Nutrition 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 230000004054 inflammatory process Effects 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 229910052500 inorganic mineral Inorganic materials 0.000 description 2
- 238000001990 intravenous administration Methods 0.000 description 2
- 239000000644 isotonic solution Substances 0.000 description 2
- 210000000867 larynx Anatomy 0.000 description 2
- 230000000670 limiting effect Effects 0.000 description 2
- 239000000314 lubricant Substances 0.000 description 2
- 239000011777 magnesium Substances 0.000 description 2
- 239000000594 mannitol Substances 0.000 description 2
- 235000010355 mannitol Nutrition 0.000 description 2
- 235000010755 mineral Nutrition 0.000 description 2
- 239000011707 mineral Substances 0.000 description 2
- 210000003928 nasal cavity Anatomy 0.000 description 2
- 235000021096 natural sweeteners Nutrition 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 239000006186 oral dosage form Substances 0.000 description 2
- 239000008188 pellet Substances 0.000 description 2
- 210000003800 pharynx Anatomy 0.000 description 2
- 235000011007 phosphoric acid Nutrition 0.000 description 2
- 229920001223 polyethylene glycol Polymers 0.000 description 2
- 239000001267 polyvinylpyrrolidone Substances 0.000 description 2
- 229920000036 polyvinylpyrrolidone Polymers 0.000 description 2
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 2
- 239000003755 preservative agent Substances 0.000 description 2
- 229940024999 proteolytic enzymes for treatment of wounds and ulcers Drugs 0.000 description 2
- 210000002345 respiratory system Anatomy 0.000 description 2
- 229910052708 sodium Inorganic materials 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- WXMKPNITSTVMEF-UHFFFAOYSA-M sodium benzoate Chemical compound [Na+].[O-]C(=O)C1=CC=CC=C1 WXMKPNITSTVMEF-UHFFFAOYSA-M 0.000 description 2
- 239000004299 sodium benzoate Substances 0.000 description 2
- 235000010234 sodium benzoate Nutrition 0.000 description 2
- 235000019812 sodium carboxymethyl cellulose Nutrition 0.000 description 2
- 229920001027 sodium carboxymethylcellulose Polymers 0.000 description 2
- 239000000600 sorbitol Substances 0.000 description 2
- 235000010356 sorbitol Nutrition 0.000 description 2
- 229960005322 streptomycin Drugs 0.000 description 2
- 235000000346 sugar Nutrition 0.000 description 2
- 238000000194 supercritical-fluid extraction Methods 0.000 description 2
- 239000000829 suppository Substances 0.000 description 2
- 239000000454 talc Substances 0.000 description 2
- 235000012222 talc Nutrition 0.000 description 2
- 229910052623 talc Inorganic materials 0.000 description 2
- 235000013616 tea Nutrition 0.000 description 2
- 239000002562 thickening agent Substances 0.000 description 2
- 210000003437 trachea Anatomy 0.000 description 2
- 201000008827 tuberculosis Diseases 0.000 description 2
- 239000000230 xanthan gum Substances 0.000 description 2
- 235000010493 xanthan gum Nutrition 0.000 description 2
- 229920001285 xanthan gum Polymers 0.000 description 2
- 229940082509 xanthan gum Drugs 0.000 description 2
- LNAZSHAWQACDHT-XIYTZBAFSA-N (2r,3r,4s,5r,6s)-4,5-dimethoxy-2-(methoxymethyl)-3-[(2s,3r,4s,5r,6r)-3,4,5-trimethoxy-6-(methoxymethyl)oxan-2-yl]oxy-6-[(2r,3r,4s,5r,6r)-4,5,6-trimethoxy-2-(methoxymethyl)oxan-3-yl]oxyoxane Chemical compound CO[C@@H]1[C@@H](OC)[C@H](OC)[C@@H](COC)O[C@H]1O[C@H]1[C@H](OC)[C@@H](OC)[C@H](O[C@H]2[C@@H]([C@@H](OC)[C@H](OC)O[C@@H]2COC)OC)O[C@@H]1COC LNAZSHAWQACDHT-XIYTZBAFSA-N 0.000 description 1
- WRIDQFICGBMAFQ-UHFFFAOYSA-N (E)-8-Octadecenoic acid Natural products CCCCCCCCCC=CCCCCCCC(O)=O WRIDQFICGBMAFQ-UHFFFAOYSA-N 0.000 description 1
- BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 description 1
- DDMOUSALMHHKOS-UHFFFAOYSA-N 1,2-dichloro-1,1,2,2-tetrafluoroethane Chemical compound FC(F)(Cl)C(F)(F)Cl DDMOUSALMHHKOS-UHFFFAOYSA-N 0.000 description 1
- 229940058015 1,3-butylene glycol Drugs 0.000 description 1
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 description 1
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- NHBKXEKEPDILRR-UHFFFAOYSA-N 2,3-bis(butanoylsulfanyl)propyl butanoate Chemical compound CCCC(=O)OCC(SC(=O)CCC)CSC(=O)CCC NHBKXEKEPDILRR-UHFFFAOYSA-N 0.000 description 1
- CHHHXKFHOYLYRE-UHFFFAOYSA-M 2,4-Hexadienoic acid, potassium salt (1:1), (2E,4E)- Chemical compound [K+].CC=CC=CC([O-])=O CHHHXKFHOYLYRE-UHFFFAOYSA-M 0.000 description 1
- LQJBNNIYVWPHFW-UHFFFAOYSA-N 20:1omega9c fatty acid Natural products CCCCCCCCCCC=CCCCCCCCC(O)=O LQJBNNIYVWPHFW-UHFFFAOYSA-N 0.000 description 1
- RTAPDZBZLSXHQQ-UHFFFAOYSA-N 8-methyl-3,7-dihydropurine-2,6-dione Chemical class N1C(=O)NC(=O)C2=C1N=C(C)N2 RTAPDZBZLSXHQQ-UHFFFAOYSA-N 0.000 description 1
- QSBYPNXLFMSGKH-UHFFFAOYSA-N 9-Heptadecensaeure Natural products CCCCCCCC=CCCCCCCCC(O)=O QSBYPNXLFMSGKH-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 244000144927 Aloe barbadensis Species 0.000 description 1
- 235000002961 Aloe barbadensis Nutrition 0.000 description 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
- 206010002091 Anaesthesia Diseases 0.000 description 1
- FMMWHPNWAFZXNH-UHFFFAOYSA-N Benz[a]pyrene Chemical compound C1=C2C3=CC=CC=C3C=C(C=C3)C2=C2C3=CC=CC2=C1 FMMWHPNWAFZXNH-UHFFFAOYSA-N 0.000 description 1
- DKPFZGUDAPQIHT-UHFFFAOYSA-N Butyl acetate Natural products CCCCOC(C)=O DKPFZGUDAPQIHT-UHFFFAOYSA-N 0.000 description 1
- 229940124697 COPD therapeutics Drugs 0.000 description 1
- 229920002134 Carboxymethyl cellulose Polymers 0.000 description 1
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
- 229910021555 Chromium Chloride Inorganic materials 0.000 description 1
- 208000014085 Chronic respiratory disease Diseases 0.000 description 1
- 235000007516 Chrysanthemum Nutrition 0.000 description 1
- 244000189548 Chrysanthemum x morifolium Species 0.000 description 1
- 244000223760 Cinnamomum zeylanicum Species 0.000 description 1
- 235000005979 Citrus limon Nutrition 0.000 description 1
- 244000131522 Citrus pyriformis Species 0.000 description 1
- 241000675108 Citrus tangerina Species 0.000 description 1
- 102000029816 Collagenase Human genes 0.000 description 1
- 108060005980 Collagenase Proteins 0.000 description 1
- 229920002261 Corn starch Polymers 0.000 description 1
- 201000003883 Cystic fibrosis Diseases 0.000 description 1
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- RGHNJXZEOKUKBD-UHFFFAOYSA-N D-gluconic acid Natural products OCC(O)C(O)C(O)C(O)C(O)=O RGHNJXZEOKUKBD-UHFFFAOYSA-N 0.000 description 1
- FEWJPZIEWOKRBE-JCYAYHJZSA-N Dextrotartaric acid Chemical compound OC(=O)[C@H](O)[C@@H](O)C(O)=O FEWJPZIEWOKRBE-JCYAYHJZSA-N 0.000 description 1
- AZJUFRDUYTYIHV-NKFKGCMQSA-N Dibenzoyl Thiamine Chemical compound C=1C=CC=CC=1C(=O)OCC\C(SC(=O)C=1C=CC=CC=1)=C(/C)N(C=O)CC1=CN=C(C)N=C1N AZJUFRDUYTYIHV-NKFKGCMQSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000194032 Enterococcus faecalis Species 0.000 description 1
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 description 1
- 239000001856 Ethyl cellulose Substances 0.000 description 1
- ZZSNKZQZMQGXPY-UHFFFAOYSA-N Ethyl cellulose Chemical compound CCOCC1OC(OC)C(OCC)C(OCC)C1OC1C(O)C(O)C(OC)C(CO)O1 ZZSNKZQZMQGXPY-UHFFFAOYSA-N 0.000 description 1
- 239000005977 Ethylene Substances 0.000 description 1
- 240000009088 Fragaria x ananassa Species 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- 235000011201 Ginkgo Nutrition 0.000 description 1
- 244000194101 Ginkgo biloba Species 0.000 description 1
- 235000008100 Ginkgo biloba Nutrition 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- 229920000084 Gum arabic Polymers 0.000 description 1
- 101001055222 Homo sapiens Interleukin-8 Proteins 0.000 description 1
- 206010020751 Hypersensitivity Diseases 0.000 description 1
- PIWKPBJCKXDKJR-UHFFFAOYSA-N Isoflurane Chemical compound FC(F)OC(Cl)C(F)(F)F PIWKPBJCKXDKJR-UHFFFAOYSA-N 0.000 description 1
- 235000010254 Jasminum officinale Nutrition 0.000 description 1
- 240000005385 Jasminum sambac Species 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
- 244000070406 Malus silvestris Species 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- GXCLVBGFBYZDAG-UHFFFAOYSA-N N-[2-(1H-indol-3-yl)ethyl]-N-methylprop-2-en-1-amine Chemical compound CN(CCC1=CNC2=C1C=CC=C2)CC=C GXCLVBGFBYZDAG-UHFFFAOYSA-N 0.000 description 1
- OVRNDRQMDRJTHS-UHFFFAOYSA-N N-acelyl-D-glucosamine Natural products CC(=O)NC1C(O)OC(CO)C(O)C1O OVRNDRQMDRJTHS-UHFFFAOYSA-N 0.000 description 1
- OVRNDRQMDRJTHS-FMDGEEDCSA-N N-acetyl-beta-D-glucosamine Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O OVRNDRQMDRJTHS-FMDGEEDCSA-N 0.000 description 1
- MBLBDJOUHNCFQT-LXGUWJNJSA-N N-acetylglucosamine Natural products CC(=O)N[C@@H](C=O)[C@@H](O)[C@H](O)[C@H](O)CO MBLBDJOUHNCFQT-LXGUWJNJSA-N 0.000 description 1
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 description 1
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 description 1
- 239000005642 Oleic acid Substances 0.000 description 1
- ZQPPMHVWECSIRJ-UHFFFAOYSA-N Oleic acid Natural products CCCCCCCCC=CCCCCCCCC(O)=O ZQPPMHVWECSIRJ-UHFFFAOYSA-N 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 240000004371 Panax ginseng Species 0.000 description 1
- 235000002789 Panax ginseng Nutrition 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 229930182555 Penicillin Natural products 0.000 description 1
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 206010035664 Pneumonia Diseases 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 229920001214 Polysorbate 60 Polymers 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 240000005809 Prunus persica Species 0.000 description 1
- 235000006040 Prunus persica var persica Nutrition 0.000 description 1
- 241000508269 Psidium Species 0.000 description 1
- 239000006146 Roswell Park Memorial Institute medium Substances 0.000 description 1
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- 239000004283 Sodium sorbate Substances 0.000 description 1
- 235000021355 Stearic acid Nutrition 0.000 description 1
- 210000001744 T-lymphocyte Anatomy 0.000 description 1
- FEWJPZIEWOKRBE-UHFFFAOYSA-N Tartaric acid Natural products [H+].[H+].[O-]C(=O)C(O)C(O)C([O-])=O FEWJPZIEWOKRBE-UHFFFAOYSA-N 0.000 description 1
- 244000299461 Theobroma cacao Species 0.000 description 1
- 235000005764 Theobroma cacao ssp. cacao Nutrition 0.000 description 1
- 235000005767 Theobroma cacao ssp. sphaerocarpum Nutrition 0.000 description 1
- 206010044302 Tracheitis Diseases 0.000 description 1
- 208000007587 Transfusion-Related Acute Lung Injury Diseases 0.000 description 1
- GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 description 1
- 241000219094 Vitaceae Species 0.000 description 1
- 229930003451 Vitamin B1 Natural products 0.000 description 1
- 229930003779 Vitamin B12 Natural products 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- 229930003427 Vitamin E Natural products 0.000 description 1
- TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 235000010489 acacia gum Nutrition 0.000 description 1
- 239000000205 acacia gum Substances 0.000 description 1
- 235000011054 acetic acid Nutrition 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 235000011037 adipic acid Nutrition 0.000 description 1
- 239000001361 adipic acid Substances 0.000 description 1
- 239000002671 adjuvant Substances 0.000 description 1
- 239000000443 aerosol Substances 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 235000010419 agar Nutrition 0.000 description 1
- 238000003915 air pollution Methods 0.000 description 1
- 150000001299 aldehydes Chemical class 0.000 description 1
- 235000010443 alginic acid Nutrition 0.000 description 1
- 239000000783 alginic acid Substances 0.000 description 1
- 229920000615 alginic acid Polymers 0.000 description 1
- 229960001126 alginic acid Drugs 0.000 description 1
- 150000004781 alginic acids Chemical class 0.000 description 1
- 208000026935 allergic disease Diseases 0.000 description 1
- 235000011399 aloe vera Nutrition 0.000 description 1
- BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 description 1
- SNAAJJQQZSMGQD-UHFFFAOYSA-N aluminum magnesium Chemical compound [Mg].[Al] SNAAJJQQZSMGQD-UHFFFAOYSA-N 0.000 description 1
- 230000037005 anaesthesia Effects 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 229940065524 anticholinergics inhalants for obstructive airway diseases Drugs 0.000 description 1
- 235000021016 apples Nutrition 0.000 description 1
- YZXBAPSDXZZRGB-DOFZRALJSA-N arachidonic acid Chemical class CCCCC\C=C/C\C=C/C\C=C/C\C=C/CCCC(O)=O YZXBAPSDXZZRGB-DOFZRALJSA-N 0.000 description 1
- 229910052785 arsenic Inorganic materials 0.000 description 1
- RQNWIZPPADIBDY-UHFFFAOYSA-N arsenic atom Chemical compound [As] RQNWIZPPADIBDY-UHFFFAOYSA-N 0.000 description 1
- 206010003246 arthritis Diseases 0.000 description 1
- 235000010323 ascorbic acid Nutrition 0.000 description 1
- 239000011668 ascorbic acid Substances 0.000 description 1
- 229960005070 ascorbic acid Drugs 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 239000000440 bentonite Substances 0.000 description 1
- 229910000278 bentonite Inorganic materials 0.000 description 1
- 235000012216 bentonite Nutrition 0.000 description 1
- SVPXDRXYRYOSEX-UHFFFAOYSA-N bentoquatam Chemical compound O.O=[Si]=O.O=[Al]O[Al]=O SVPXDRXYRYOSEX-UHFFFAOYSA-N 0.000 description 1
- 229940124748 beta 2 agonist Drugs 0.000 description 1
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 235000008429 bread Nutrition 0.000 description 1
- 229940124630 bronchodilator Drugs 0.000 description 1
- 239000000168 bronchodilator agent Substances 0.000 description 1
- 244000309464 bull Species 0.000 description 1
- 235000019437 butane-1,3-diol Nutrition 0.000 description 1
- 235000014121 butter Nutrition 0.000 description 1
- 235000001046 cacaotero Nutrition 0.000 description 1
- 239000004301 calcium benzoate Substances 0.000 description 1
- 235000010237 calcium benzoate Nutrition 0.000 description 1
- FNAQSUUGMSOBHW-UHFFFAOYSA-H calcium citrate Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O.[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O FNAQSUUGMSOBHW-UHFFFAOYSA-H 0.000 description 1
- 239000001354 calcium citrate Substances 0.000 description 1
- 159000000007 calcium salts Chemical class 0.000 description 1
- MCFVRESNTICQSJ-RJNTXXOISA-L calcium sorbate Chemical compound [Ca+2].C\C=C\C=C\C([O-])=O.C\C=C\C=C\C([O-])=O MCFVRESNTICQSJ-RJNTXXOISA-L 0.000 description 1
- 239000004303 calcium sorbate Substances 0.000 description 1
- 235000010244 calcium sorbate Nutrition 0.000 description 1
- HZQXCUSDXIKLGS-UHFFFAOYSA-L calcium;dibenzoate;trihydrate Chemical compound O.O.O.[Ca+2].[O-]C(=O)C1=CC=CC=C1.[O-]C(=O)C1=CC=CC=C1 HZQXCUSDXIKLGS-UHFFFAOYSA-L 0.000 description 1
- 125000004432 carbon atom Chemical group C* 0.000 description 1
- 229960004424 carbon dioxide Drugs 0.000 description 1
- 229910002091 carbon monoxide Inorganic materials 0.000 description 1
- 235000014171 carbonated beverage Nutrition 0.000 description 1
- 235000010948 carboxy methyl cellulose Nutrition 0.000 description 1
- 239000008112 carboxymethyl-cellulose Substances 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 150000001765 catechin Chemical class 0.000 description 1
- ADRVNXBAWSRFAJ-UHFFFAOYSA-N catechin Natural products OC1Cc2cc(O)cc(O)c2OC1c3ccc(O)c(O)c3 ADRVNXBAWSRFAJ-UHFFFAOYSA-N 0.000 description 1
- 235000005487 catechin Nutrition 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 239000006143 cell culture medium Substances 0.000 description 1
- 239000000460 chlorine Substances 0.000 description 1
- 229910052801 chlorine Inorganic materials 0.000 description 1
- 239000000812 cholinergic antagonist Substances 0.000 description 1
- QSWDMMVNRMROPK-UHFFFAOYSA-K chromium(3+) trichloride Chemical compound [Cl-].[Cl-].[Cl-].[Cr+3] QSWDMMVNRMROPK-UHFFFAOYSA-K 0.000 description 1
- 235000019504 cigarettes Nutrition 0.000 description 1
- 235000017803 cinnamon Nutrition 0.000 description 1
- FDJOLVPMNUYSCM-WZHZPDAFSA-L cobalt(3+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+3].N#[C-].N([C@@H]([C@]1(C)[N-]\C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C(\C)/C1=N/C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C\C1=N\C([C@H](C1(C)C)CCC(N)=O)=C/1C)[C@@H]2CC(N)=O)=C\1[C@]2(C)CCC(=O)NC[C@@H](C)OP([O-])(=O)O[C@H]1[C@@H](O)[C@@H](N2C3=CC(C)=C(C)C=C3N=C2)O[C@@H]1CO FDJOLVPMNUYSCM-WZHZPDAFSA-L 0.000 description 1
- 229960002424 collagenase Drugs 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 239000008120 corn starch Substances 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 239000000287 crude extract Substances 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000000151 deposition Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- UMNKXPULIDJLSU-UHFFFAOYSA-N dichlorofluoromethane Chemical compound FC(Cl)Cl UMNKXPULIDJLSU-UHFFFAOYSA-N 0.000 description 1
- 229940099364 dichlorofluoromethane Drugs 0.000 description 1
- 229940087091 dichlorotetrafluoroethane Drugs 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000007884 disintegrant Substances 0.000 description 1
- 239000008298 dragée Substances 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 229940032049 enterococcus faecalis Drugs 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 235000019325 ethyl cellulose Nutrition 0.000 description 1
- 229920001249 ethyl cellulose Polymers 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 230000037406 food intake Effects 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 239000001530 fumaric acid Substances 0.000 description 1
- 235000011087 fumaric acid Nutrition 0.000 description 1
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 description 1
- 229910052732 germanium Inorganic materials 0.000 description 1
- GNPVGFCGXDBREM-UHFFFAOYSA-N germanium atom Chemical compound [Ge] GNPVGFCGXDBREM-UHFFFAOYSA-N 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 235000012208 gluconic acid Nutrition 0.000 description 1
- 239000000174 gluconic acid Substances 0.000 description 1
- 235000001727 glucose Nutrition 0.000 description 1
- 235000021021 grapes Nutrition 0.000 description 1
- 239000004519 grease Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- FUZZWVXGSFPDMH-UHFFFAOYSA-N hexanoic acid Chemical compound CCCCCC(O)=O FUZZWVXGSFPDMH-UHFFFAOYSA-N 0.000 description 1
- 238000010562 histological examination Methods 0.000 description 1
- 235000012907 honey Nutrition 0.000 description 1
- 238000007602 hot air drying Methods 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- WGCNASOHLSPBMP-UHFFFAOYSA-N hydroxyacetaldehyde Natural products OCC=O WGCNASOHLSPBMP-UHFFFAOYSA-N 0.000 description 1
- 239000001866 hydroxypropyl methyl cellulose Substances 0.000 description 1
- 235000010979 hydroxypropyl methyl cellulose Nutrition 0.000 description 1
- 229920003088 hydroxypropyl methyl cellulose Polymers 0.000 description 1
- UFVKGYZPFZQRLF-UHFFFAOYSA-N hydroxypropyl methyl cellulose Chemical compound OC1C(O)C(OC)OC(CO)C1OC1C(O)C(O)C(OC2C(C(O)C(OC3C(C(O)C(O)C(CO)O3)O)C(CO)O2)O)C(CO)O1 UFVKGYZPFZQRLF-UHFFFAOYSA-N 0.000 description 1
- 230000009610 hypersensitivity Effects 0.000 description 1
- 230000008105 immune reaction Effects 0.000 description 1
- 210000004969 inflammatory cell Anatomy 0.000 description 1
- 238000007918 intramuscular administration Methods 0.000 description 1
- 229940082629 iron antianemic preparations Drugs 0.000 description 1
- NPFOYSMITVOQOS-UHFFFAOYSA-K iron(III) citrate Chemical compound [Fe+3].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NPFOYSMITVOQOS-UHFFFAOYSA-K 0.000 description 1
- 239000002085 irritant Substances 0.000 description 1
- 231100000021 irritant Toxicity 0.000 description 1
- 230000007794 irritation Effects 0.000 description 1
- 229960002725 isoflurane Drugs 0.000 description 1
- QXJSBBXBKPUZAA-UHFFFAOYSA-N isooleic acid Natural products CCCCCCCC=CCCCCCCCCC(O)=O QXJSBBXBKPUZAA-UHFFFAOYSA-N 0.000 description 1
- 235000015110 jellies Nutrition 0.000 description 1
- 239000008274 jelly Substances 0.000 description 1
- VMPHSYLJUKZBJJ-UHFFFAOYSA-N lauric acid triglyceride Natural products CCCCCCCCCCCC(=O)OCC(OC(=O)CCCCCCCCCCC)COC(=O)CCCCCCCCCCC VMPHSYLJUKZBJJ-UHFFFAOYSA-N 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 229940040145 liniment Drugs 0.000 description 1
- 239000000865 liniment Substances 0.000 description 1
- 235000014666 liquid concentrate Nutrition 0.000 description 1
- 239000006210 lotion Substances 0.000 description 1
- 235000001055 magnesium Nutrition 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 159000000003 magnesium salts Chemical class 0.000 description 1
- 239000001630 malic acid Substances 0.000 description 1
- 235000011090 malic acid Nutrition 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 239000013028 medium composition Substances 0.000 description 1
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 1
- 229910052753 mercury Inorganic materials 0.000 description 1
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- LXCFILQKKLGQFO-UHFFFAOYSA-N methylparaben Chemical compound COC(=O)C1=CC=C(O)C=C1 LXCFILQKKLGQFO-UHFFFAOYSA-N 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 239000002480 mineral oil Substances 0.000 description 1
- 235000010446 mineral oil Nutrition 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000004400 mucous membrane Anatomy 0.000 description 1
- 229950006780 n-acetylglucosamine Drugs 0.000 description 1
- 235000019462 natural additive Nutrition 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 229910017604 nitric acid Inorganic materials 0.000 description 1
- 235000012149 noodles Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- QIQXTHQIDYTFRH-UHFFFAOYSA-N octadecanoic acid Chemical compound CCCCCCCCCCCCCCCCCC(O)=O QIQXTHQIDYTFRH-UHFFFAOYSA-N 0.000 description 1
- OQCDKBAXFALNLD-UHFFFAOYSA-N octadecanoic acid Natural products CCCCCCCC(C)CCCCCCCCC(O)=O OQCDKBAXFALNLD-UHFFFAOYSA-N 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 235000019198 oils Nutrition 0.000 description 1
- 239000002674 ointment Substances 0.000 description 1
- ZQPPMHVWECSIRJ-KTKRTIGZSA-N oleic acid Chemical compound CCCCCCCC\C=C/CCCCCCCC(O)=O ZQPPMHVWECSIRJ-KTKRTIGZSA-N 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 235000019449 other food additives Nutrition 0.000 description 1
- 230000036542 oxidative stress Effects 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000012188 paraffin wax Substances 0.000 description 1
- 239000006201 parenteral dosage form Substances 0.000 description 1
- 235000015927 pasta Nutrition 0.000 description 1
- 239000006072 paste Substances 0.000 description 1
- 239000001814 pectin Substances 0.000 description 1
- 229920001277 pectin Polymers 0.000 description 1
- 235000010987 pectin Nutrition 0.000 description 1
- 229940049954 penicillin Drugs 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 125000005575 polycyclic aromatic hydrocarbon group Chemical group 0.000 description 1
- 229920005862 polyol Polymers 0.000 description 1
- 150000003077 polyols Chemical class 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- XAEFZNCEHLXOMS-UHFFFAOYSA-M potassium benzoate Chemical compound [K+].[O-]C(=O)C1=CC=CC=C1 XAEFZNCEHLXOMS-UHFFFAOYSA-M 0.000 description 1
- 239000004300 potassium benzoate Substances 0.000 description 1
- 235000010235 potassium benzoate Nutrition 0.000 description 1
- 229940103091 potassium benzoate Drugs 0.000 description 1
- 239000004302 potassium sorbate Substances 0.000 description 1
- 235000010241 potassium sorbate Nutrition 0.000 description 1
- 229940069338 potassium sorbate Drugs 0.000 description 1
- 229920001592 potato starch Polymers 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000003380 propellant Substances 0.000 description 1
- QELSKZZBTMNZEB-UHFFFAOYSA-N propylparaben Chemical compound CCCOC(=O)C1=CC=C(O)C=C1 QELSKZZBTMNZEB-UHFFFAOYSA-N 0.000 description 1
- 229960003415 propylparaben Drugs 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 239000003642 reactive oxygen metabolite Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 238000010992 reflux Methods 0.000 description 1
- 230000003014 reinforcing effect Effects 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- MNDBXUUTURYVHR-UHFFFAOYSA-N roflumilast Chemical group FC(F)OC1=CC=C(C(=O)NC=2C(=CN=CC=2Cl)Cl)C=C1OCC1CC1 MNDBXUUTURYVHR-UHFFFAOYSA-N 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 229910052711 selenium Inorganic materials 0.000 description 1
- 239000011669 selenium Substances 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 239000000779 smoke Substances 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 235000010413 sodium alginate Nutrition 0.000 description 1
- 239000000661 sodium alginate Substances 0.000 description 1
- 229940005550 sodium alginate Drugs 0.000 description 1
- 229960003885 sodium benzoate Drugs 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 235000002639 sodium chloride Nutrition 0.000 description 1
- RYYKJJJTJZKILX-UHFFFAOYSA-M sodium octadecanoate Chemical compound [Na+].CCCCCCCCCCCCCCCCCC([O-])=O RYYKJJJTJZKILX-UHFFFAOYSA-M 0.000 description 1
- 159000000000 sodium salts Chemical class 0.000 description 1
- LROWVYNUWKVTCU-STWYSWDKSA-M sodium sorbate Chemical compound [Na+].C\C=C\C=C\C([O-])=O LROWVYNUWKVTCU-STWYSWDKSA-M 0.000 description 1
- 235000019250 sodium sorbate Nutrition 0.000 description 1
- 239000004071 soot Substances 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 238000001694 spray drying Methods 0.000 description 1
- 239000012128 staining reagent Substances 0.000 description 1
- 239000008117 stearic acid Substances 0.000 description 1
- 239000008227 sterile water for injection Substances 0.000 description 1
- 150000003431 steroids Chemical class 0.000 description 1
- 235000021012 strawberries Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 239000000375 suspending agent Substances 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 235000002906 tartaric acid Nutrition 0.000 description 1
- 239000011975 tartaric acid Substances 0.000 description 1
- 150000003505 terpenes Chemical class 0.000 description 1
- 235000007586 terpenes Nutrition 0.000 description 1
- 229960003495 thiamine Drugs 0.000 description 1
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 1
- 235000010384 tocopherol Nutrition 0.000 description 1
- 229930003799 tocopherol Natural products 0.000 description 1
- 229960001295 tocopherol Drugs 0.000 description 1
- 239000011732 tocopherol Substances 0.000 description 1
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 description 1
- 235000013337 tricalcium citrate Nutrition 0.000 description 1
- CYRMSUTZVYGINF-UHFFFAOYSA-N trichlorofluoromethane Chemical compound FC(Cl)(Cl)Cl CYRMSUTZVYGINF-UHFFFAOYSA-N 0.000 description 1
- 229940029284 trichlorofluoromethane Drugs 0.000 description 1
- 229960004418 trolamine Drugs 0.000 description 1
- 241000712461 unidentified influenza virus Species 0.000 description 1
- 238000001291 vacuum drying Methods 0.000 description 1
- 229910052720 vanadium Inorganic materials 0.000 description 1
- GPPXJZIENCGNKB-UHFFFAOYSA-N vanadium Chemical compound [V]#[V] GPPXJZIENCGNKB-UHFFFAOYSA-N 0.000 description 1
- 235000015112 vegetable and seed oil Nutrition 0.000 description 1
- 239000008158 vegetable oil Substances 0.000 description 1
- 239000000052 vinegar Substances 0.000 description 1
- 235000021419 vinegar Nutrition 0.000 description 1
- 230000009385 viral infection Effects 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 235000010374 vitamin B1 Nutrition 0.000 description 1
- 239000011691 vitamin B1 Substances 0.000 description 1
- 235000019163 vitamin B12 Nutrition 0.000 description 1
- 239000011715 vitamin B12 Substances 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- 235000019165 vitamin E Nutrition 0.000 description 1
- 239000011709 vitamin E Substances 0.000 description 1
- 229940046009 vitamin E Drugs 0.000 description 1
- 235000012431 wafers Nutrition 0.000 description 1
- 239000001993 wax Substances 0.000 description 1
- 229940100445 wheat starch Drugs 0.000 description 1
- 239000008096 xylene Substances 0.000 description 1
- 239000000811 xylitol Substances 0.000 description 1
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 description 1
- 235000010447 xylitol Nutrition 0.000 description 1
- 229960002675 xylitol Drugs 0.000 description 1
- 235000013618 yogurt Nutrition 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- GVJHHUAWPYXKBD-IEOSBIPESA-N α-tocopherol Chemical compound OC1=C(C)C(C)=C2O[C@@](CCC[C@H](C)CCC[C@H](C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-IEOSBIPESA-N 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/82—Theaceae (Tea family), e.g. camellia
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/37—Celastraceae (Staff-tree or Bittersweet family), e.g. tripterygium or spindletree
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
- A61K36/732—Chaenomeles, e.g. flowering quince
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P11/00—Drugs for disorders of the respiratory system
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
- A23V2200/314—Foods, ingredients or supplements having a functional effect on health having an effect on lung or respiratory system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2300/00—Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Botany (AREA)
- Mycology (AREA)
- Alternative & Traditional Medicine (AREA)
- Epidemiology (AREA)
- Microbiology (AREA)
- Medical Informatics (AREA)
- Biotechnology (AREA)
- Pulmonology (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Nutrition Science (AREA)
- General Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
본 발명은 화살나무 추출물을 이용한 만성폐쇄성폐질환 등의 폐질환 개선용 조성물을 개시한다. 화살나무 추출물은 PMA(phorbol myristate acetate)에 의해 유도된 호중구 세포외 트랩(Neutrophil extracellular traps)의 네토시스(Netosis) 활성화를 억제하고, 점액질을 생성하는 기관지 상피세포주인 NCI-H292 세포의 CSE에 의한 염증성 사이토카인인 IL-8의 발현을 억제하며, CES와 LPS(lipopolysaccaride)에 의해 활성된 폐포 대식세포인 MH-S 세포의 염증성 사이토카인인 MIP-2의 발현을 억제하는 활성을 가진다. 또한, CSE와 PPE로 유도된 호흡기 질환 마우스 모델에서 폐 조직 손상을 억제하고, BALF 내에 총 세포(Total cell), 대식세포(macrophages), 림프구(Lymphocytes), 호산구(Eosinophil), 호중구(Neutrophil) 등의 면역세포 침윤을 억제하며, BALF 및 폐 조직에서 염증성 사이토카인과 케모카인 분비를 억제하는 활성을 가진다.The present invention discloses a composition for improving lung diseases such as chronic obstructive pulmonary disease using an arrow tree extract. Arrow tree extract inhibits the activation of netosis of neutrophil extracellular traps induced by PMA (phorbol myristate acetate), and is induced by CSE of NCI-H292 cells, a bronchial epithelial cell line that produces mucus. It inhibits the expression of IL-8, an inflammatory cytokine, and has the activity of inhibiting the expression of MIP-2, an inflammatory cytokine, in MH-S cells, alveolar macrophages activated by CES and LPS (lipopolysaccaride). In addition, it inhibits lung tissue damage in a mouse model of respiratory disease induced by CSE and PPE, and includes total cells, macrophages, lymphocytes, eosinophils, and neutrophils in BALF. It inhibits the invasion of immune cells and has the activity of inhibiting the secretion of inflammatory cytokines and chemokines in BALF and lung tissue.
Description
본 발명은 화살나무 추출물을 이용한 만성폐쇄성 폐질환 개선용 조성물에 관한 것이다.The present invention relates to a composition for improving chronic obstructive pulmonary disease using an arrow tree extract.
만성 폐쇄성 폐질환(chronic obstructive pulmonary disease, COPD)은 기침, 객담, 호흡 곤란, 호기 유속의 감소, 가스 교환의 장애 등을 보이는 만성 기도 질환으로서, 해마다 전세계적으로 그 발병 인구가 증가하고 있으며, 2020년에는 인류의 사망원인 중 3번째 원인이 될 것으로 예측되고 있다(Am J Respir Crit Care Med, 2013, 187:347-365; Am J Respir Crit Care Med, 2009, 180:396-406).Chronic obstructive pulmonary disease (COPD) is a chronic airway disease showing cough, sputum, shortness of breath, decreased expiratory flow, and impaired gas exchange. It is predicted to be the third cause of death in humans in 2012 (Am J Respir Crit Care Med, 2013, 187:347-365; Am J Respir Crit Care Med, 2009, 180:396-406).
COPD는 흡연, 대기오염, 화학물질, 직업성 인자, 유전적 소인 등 다양한 원인에 의해서 발병하는데, 이중 흡연이 주요한 원인으로 지목되고 있으며, 실제 COPD 환자의 80% 이상이 흡연자로 밝혀진 바 있다(Biol Pharm Bull, 2012, 35:1752-1760). COPD의 발병 기전에는 염증, 폐에서의 단백분해효소 활성화, 산화적 스트레스(oxidative stress) 등이 관여하며, 염증에 관여하는 세포는 주로 호중구와 대식세포, T 림프구 등이다. 이러한 염증세포들은 활성산소종(reactive oxygen species), 다양한 염증성 사이토카인, 조직 손상을 야기하는 여러 단백분해 효소들을 생성한다(대한결핵 및 호흡기학회 호흡기학 서울: 군자출판사; 2007, p 301-5;Am J Respir Crit Care Med, 1997 155, 1441-1447; Am J Physiol Lung CellMol Physiol, 2010, 298:L262-L269). 특히, 호중구는 세포 외로 엘라스타아제, 콜라게나아제, MPO(myeloperoxidase)와 같은 단백분해효소(proteases), 아라키돈산대사물(arachidonate), 활성산소종(reactive oxygen free radical) 등의 물질을 분비하여 폐손상을 야기함으로써 COPD가 발병하는데 중요한 역할을 하는 것으로 알려져 있으며, 따라서 COPD 치료제의 개발에 있어 중요한 표적이 되고 있다(Am J Respir Cell Mol Biol, 2013, 48:531-539; Eur Respir J, 1998, 12:1200-1208).COPD is caused by a variety of causes, such as smoking, air pollution, chemicals, occupational factors, and genetic predisposition, and smoking is the main cause, and more than 80% of COPD patients have been found to be smokers (Biol Pharm Bull, 2012, 35:1752-1760). The pathogenesis of COPD involves inflammation, activation of proteolytic enzymes in the lungs, and oxidative stress, and the cells involved in inflammation are mainly neutrophils, macrophages, and T lymphocytes. These inflammatory cells produce reactive oxygen species, various inflammatory cytokines, and various proteolytic enzymes that cause tissue damage (Korean Tuberculosis and Respiratory Society, Respiratory Science Seoul: Gunja Publishing Co.; 2007, p 301-5;Am J Respir Crit Care Med, 1997 155, 1441-1447; Am J Physiol Lung CellMol Physiol, 2010, 298:L262-L269). In particular, neutrophils secrete substances such as elastase, collagenase, proteases such as MPO (myeloperoxidase), arachidonic acid metabolites, and reactive oxygen free radicals to the lungs. It is known to play an important role in the onset of COPD by causing damage, and therefore, it has become an important target in the development of COPD therapeutics (Am J Respir Cell Mol Biol, 2013, 48:531-539; Eur Respir J, 1998, 12:1200-1208).
최근 호중구 세포외 트랩(Neutrophil extracellular traps)의 네토시스(Netosis) 활성화가 COPD를 비롯한 만성 폐/호흡기 질환의 발병 기전에 관여하는 것으로 보고되어 있다(PLoS One. 2014 May 15;9(5):e97784.; Respir Res. 2015 May 22;16:59.; J Immunol Res. 2017;2017:6710278; Respirology. 2016 Apr; 21(3):467-75).Recently, it has been reported that the activation of netosis of neutrophil extracellular traps is involved in the pathogenesis of chronic lung/respiratory diseases including COPD (PLoS One. 2014 May 15;9(5):e97784) .; Respir Res. 2015 May 22;16:59.; J Immunol Res. 2017;2017:6710278; Respirology. 2016 Apr; 21(3):467-75).
이제까지 COPD 등을 직접적으로 개선시키는 약물은 보고된 바 없으며, 현재의 COPD 치료제로는 증상과 합병증을 감소시키기 위한 것으로 기관지확장제(β2-작용제, 항콜린제, methylxanthines)와 스테로이드제(흡입, 경구) 등이 주로 사용되고 있다.So far, no drugs have been reported to directly improve COPD, and the current treatment for COPD is to reduce symptoms and complications, such as bronchodilators (β2-agonists, anticholinergics, methylxanthines) and steroids (inhalation, oral). Is mainly used.
본 발명의 목적은 화살나무 추출물을 이용한 호흡기 기능 강화 및 호흡기 질환 개선용 식품조성물, 상기 식품 조성물을 포함하는 건강기능식품 및 화살나무 추출물을 이용한 호흡기 질환 예방 또는 치료용 약제학적 조성물을 제공하는 데 있다. 본 발명의 다른 목적이나 구체적인 목적은 이하에서 제시될 것이다.It is an object of the present invention to provide a food composition for enhancing respiratory function and improving respiratory diseases using an arrow tree extract, a health functional food comprising the food composition, and a pharmaceutical composition for preventing or treating respiratory disease using an arrow tree extract . Other or specific objects of the present invention will be presented below.
본 발명자들은 아래의 실시예 및 실험예에서 확인되는 바와 같이, 화살나무 추출물이 PMA(phorbol myristate acetate)에 의해 유도된 호중구 세포외 트랩(Neutrophil extracellular traps)의 네토시스(Netosis) 활성화를 억제하고, 기관지 상피세포주인 NCI-H292 세포의 CSE에 의한 염증성 사이토카인인 IL-8의 발현을 억제하며, CES와 LPS(lipopolysaccharide) 의해 활성된 폐포 대식세포인 MH-S 세포에서도 염증성 사이토카인인 MIP-2의 발현을 억제함을 확인할 수 있었다.The present inventors, as confirmed in the Examples and Experimental Examples below, the arrow tree extract inhibits the activation of netosis of neutrophil extracellular traps induced by PMA (phorbol myristate acetate), Inhibits the expression of IL-8, an inflammatory cytokine, by CSE in NCI-H292 cells, a bronchial epithelial cell line, and MIP-2, an inflammatory cytokine, also in MH-S cells, alveolar macrophages activated by CES and LPS (lipopolysaccharide). It was confirmed that it inhibits the expression of.
전술한 바를 고려할 때, 본 발명은, 일 측면에 있어서, 화살나무(Euonymus alatus) 추출물을 유효성분으로 포함하는 COPD 개선용 조성물로 파악될 수 있으며, 또 다른 측면에 있어서는 화살나무 추출물을 유효성분으로 포함하는 호흡기 기능 강화 및 호흡기 질환 개선용 조성물로도 파악할 수 있으며, 또한 본 발명은 화살나무 추출물을 유효성분으로 포함하는, 기침, 객담, 호흡 곤란, 기도 과민성, 기도 폐쇄, 점액 과분비, 호기 유속의 감소 및/또는 가스 교환의 장애 증상을 수반하는 폐질환 개선용 조성물로도 파악할 수 있다. 이러한 폐질환은 앞서 언급한 COPD 뿐 아니라 미만성 간질성 폐질환, 급성호흡곤란증후군(acute respiratory distress syndrome, ARDS), 급성 폐손상 등을 포함하며, 발생 원인을 불문하고 앞서 언급한 흡연, 대기오염, 유전적 소인 등에 의한 폐질환 이외에 특히, 최근 문제가 되고 있는 미세먼지에 의한 폐질환을 포함한다. 검댕, 생물체 유기탄소 등 탄소성분과 염소, 질산, 암모늄, 나트륨, 칼슘 등의 이온성분, 납, 비소, 수은과 같은 금속성분, 벤조피렌 등과 같은 다환방향족 탄화수소 등 다양한 성분을 포함하고 있는 미세먼지는 상기도, 기관지, 소기도, 폐포 등에 침착하여 천식, COPD 등 다양한 폐질환을 일으키는 것으로 알려져 있다(J Korean Med Assoc, 2014; 57: 763-768).In consideration of the above, the present invention, in one aspect, can be understood as a composition for improving COPD comprising an extract of Euonymus alatus as an active ingredient, and in another aspect, an extract of an arrow tree is used as an active ingredient. It can also be understood as a composition for enhancing respiratory function and improving respiratory diseases, and the present invention includes cough, sputum, shortness of breath, airway irritability, airway obstruction, mucus hypersecretion, and expiratory flow rate, which contains an arrow tree extract as an active ingredient. It can also be recognized as a composition for improving lung disease accompanying symptoms of reduction and/or impaired gas exchange. These lung diseases include not only the aforementioned COPD, but also diffuse interstitial lung disease, acute respiratory distress syndrome (ARDS), acute lung injury, etc. In addition to pulmonary diseases due to genetic predisposition, in particular, it includes pulmonary diseases due to fine dust, which is a problem recently. Fine dust containing various components such as carbon components such as soot and organic carbon in living organisms, ionic components such as chlorine, nitric acid, ammonium, sodium, calcium, metal components such as lead, arsenic, and mercury, and polycyclic aromatic hydrocarbons such as benzopyrene, are described above. It is known to cause various lung diseases such as asthma and COPD by depositing in the islands, bronchi, small airways, and alveoli (J Korean Med Assoc, 2014; 57: 763-768).
본 명세서에서, "추출물"이란 추출 대상인 식물의 줄기, 잎, 열매, 꽃, 뿌리, 이들의 혼합물 등을 물, 탄소수 1 내지 4의 저급 알콜(메탄올, 에탄올, 부탄올 등), 메틸렌클로라이드, 에틸렌, 아세톤, 헥산, 에테르, 클로로포름, 에틸 아세테이트, 부틸아세테이트, N,N-디메틸포름아미드(DMF), 디메틸설폭사이드(DMSO), 1,3-부틸렌글리콜, 프로필렌글리콜 또는 이들의 혼합 용매를 사용하여 침출하여 얻어진 추출물, 이산화탄소, 펜탄 등 초임계 추출 용매를 사용하여 얻어진 추출물 또는 그 추출물을 분획하여 얻어진 분획물을 의미하며, 추출 방법은 활성물질의 극성, 추출 정도, 보존 정도를 고려하여 냉침, 환류, 가온, 초음파 방사, 초임계 추출 등 임의의 방법을 적용할 수 있다. 분획된 추출물의 경우 추출물을 특정 용매에 현탁시킨 후 극성이 다른 용매와 혼합·정치시켜 얻은 분획물, 상기 조추출물을 실리카겔 등이 충진된 칼럼에 흡착시킨 후 소수성 용매, 친수성 용매 또는 이들의 혼합 용매를 이동상으로 하여 얻은 분획물을 포함하는 의미이다. 또한 상기 추출물의 의미에는 동결건조, 진공건조, 열풍건조, 분무건조 등의 방식으로 추출 용매가 제거된 농축된 액상의 추출물 또는 고형상의 추출물이 포함된다. 바람직하게는 추출용매로서 물, 에탄올 또는 이들의 혼합 용매를 사용하여 얻어진 추출물, 더 바람직하게는 추출용매로서 물과 에탄올의 혼합 용매를 사용하여 얻어진 추출물을 의미한다.In the present specification, the term "extract" refers to the stem, leaves, fruits, flowers, roots, and mixtures thereof of the plant to be extracted, water, lower alcohols having 1 to 4 carbon atoms (methanol, ethanol, butanol, etc.), methylene chloride, ethylene, Using acetone, hexane, ether, chloroform, ethyl acetate, butyl acetate, N,N-dimethylformamide (DMF), dimethyl sulfoxide (DMSO), 1,3-butylene glycol, propylene glycol, or a mixed solvent thereof It refers to an extract obtained by leaching, an extract obtained using a supercritical extraction solvent such as carbon dioxide, pentane, or a fraction obtained by fractionating the extract, and the extraction method includes cold sedimentation, reflux, and Any method, such as heating, ultrasonic radiation, and supercritical extraction, can be applied. In the case of a fractionated extract, a fraction obtained by suspending the extract in a specific solvent and then mixing and policing it with a solvent having a different polarity, the crude extract is adsorbed on a column filled with silica gel, etc., and then a hydrophobic solvent, a hydrophilic solvent, or a mixed solvent thereof is added. It means including the fraction obtained as a mobile phase. In addition, the meaning of the extract includes a concentrated liquid extract or a solid extract from which the extraction solvent has been removed by a method such as freeze drying, vacuum drying, hot air drying, spray drying, or the like. Preferably, it means an extract obtained by using water, ethanol or a mixed solvent thereof as an extraction solvent, more preferably an extract obtained by using a mixed solvent of water and ethanol as an extraction solvent.
또 본 명세서에서 "유효성분"이란 단독으로 목적하는 활성을 나타내거나 또는 그 자체는 활성이 없는 담체와 함께 활성을 나타낼 수 있는 성분을 의미한다.In addition, in the present specification, the term "active ingredient" refers to an ingredient that can exhibit a desired activity alone or exhibit activity with a carrier that is not itself active.
본 발명에서 용어 "호흡기 기능 강화"는 비강, 인두, 후두, 기관, 기관지 및 폐 등 호흡 기관의 본래 기능을 건강한 상태로 유지시키거나, 흡연, 미세먼지, 호중구 네토시스 활성화 또는 기타 호흡기 질환 등에 따른 증상으로 인해 저하된 호흡 기관의 기능을 본래 건강한 상태로 개선시키는 모든 행위를 의미한다.In the present invention, the term "respiratory function enhancement" refers to maintaining the original function of the respiratory organs such as the nasal cavity, pharynx, larynx, trachea, bronchi, and lungs in a healthy state, or according to smoking, fine dust, activation of neutrophil netosis, or other respiratory diseases. It refers to any action that improves the function of the respiratory tract, which has been deteriorated due to symptoms, to its original healthy state.
또한, 본 발명에서 상기 "호흡기 질환"은 비강, 인두, 후두, 기관, 기관지 및 폐 등 개체의 호흡 기관에 발생하는 질환을 의미하는 것으로, 구체적으로 상기 호흡기 질환은 흡연 또는 미세먼지에 의한 호흡기 질환; 또는 네토시스(Netosis)를 수반하는 폐질환을 의미할 수 있으나, 이에 특별히 제한되는 것은 아니다. 보다 구체적으로, 상기 호흡기 질환은 객담, 호흡 곤란, 기도 과민성, 기도 폐쇄, 점액 과분비, 호기 유속의 감소 및/또는 가스 교환의 장애 증상을 수반하는 폐질환을 의미할 수 있고, 보다 더 구체적으로 천식, 만성폐쇄성폐질환(COPD), 기관염(tracheitis), 기관지염(bronchitis), 미만성 간질성 폐질환, 급성 호흡곤란 증후군(acute respiratory distress syndrome, ARDS), 급성 폐손상, 낭성 섬유증(cystic fibrosis), 모세기관지염(bronchiolitis), 인플루엔자 바이러스 감염증(influenza virus infection), 폐렴(pneumonia), 결핵(tuberculosis) 및 수혈관련급성폐장애(transfusion-related acute lung injury)로 구성된 군으로부터 선택되는 하나 이상을 의미할 수 있으며, 가장 구체적으로는 COPD를 의미할 수 있다. 한편, 최근 호중구 세포 외 트랩(Neutrophil extracellular traps)의 네토시스(Netosis) 활성화가 COPD를 비롯한 만성 호흡기 질환의 발병 기전에 관여하는 것으로 보고되어 있다.In addition, in the present invention, the "respiratory disease" refers to a disease occurring in the respiratory tract of an individual such as the nasal cavity, pharynx, larynx, trachea, bronchi and lungs, and specifically, the respiratory disease is a respiratory disease caused by smoking or fine dust ; Or, it may mean a lung disease accompanying Netosis, but is not particularly limited thereto. More specifically, the respiratory disease may mean a pulmonary disease accompanied by symptoms of sputum, shortness of breath, airway irritability, airway obstruction, mucus hypersecretion, decrease in expiratory flow rate and/or impaired gas exchange, and more specifically asthma , Chronic obstructive pulmonary disease (COPD), tracheitis, bronchitis, diffuse interstitial lung disease, acute respiratory distress syndrome (ARDS), acute lung injury, cystic fibrosis, capillary It may mean one or more selected from the group consisting of bronchitis, influenza virus infection, pneumonia, tuberculosis, and transfusion-related acute lung injury. , Most specifically, may mean COPD. Meanwhile, it has been recently reported that the activation of netosis of neutrophil extracellular traps is involved in the pathogenesis of chronic respiratory diseases including COPD.
본 발명의 조성물에서 그 유효성분은 COPD 개선 활성, 기타 폐질환 개선 활성, 호흡기 기능 강화 및 호흡기 질환 개선 활성을 나타낼 수 있는 한, 그 구체적 용도, 제형, 배합 목적 등에 따라 임의의 양(유효량)으로 포함될 수 있는데, 통상적인 유효량은 조성물 전체 중량을 기준으로 할 때 0.001 중량 % 내지 20.0 중량 % 범위 내에서 결정될 것이다. 여기서 "유효량"이란 그 적용 대상인 포유동물 바람직하게는 사람에게 의료 전문가 등의 제언에 의한 투여 기간 동안 본 발명의 조성물이 투여될 때, COPD 개선 효과 등 의도한 기능적·약리학적 효과를 나타낼 수 있는, 본 발명의 조성물에 포함되는 유효성분의 양을 말한다. 이러한 유효량은 당업자의 통상의 능력 범위 내에서 실험적으로 결정될 수 있다.As long as the active ingredient in the composition of the present invention can exhibit COPD improvement activity, other lung disease improvement activity, respiratory function enhancement, and respiratory disease improvement activity, the active ingredient may be in an arbitrary amount (effective amount) according to the specific use, formulation, purpose of combination, etc. Wherein, a typical effective amount will be determined within the range of 0.001% to 20.0% by weight based on the total weight of the composition. Here, "effective amount" refers to the intended functional and pharmacological effect, such as COPD improvement effect, when the composition of the present invention is administered to a mammal, preferably a human, to which the composition of the present invention is administered during the administration period according to the advice of a medical expert, etc. It refers to the amount of the active ingredient contained in the composition of the present invention. Such effective amounts can be determined empirically within the range of ordinary skill in the art.
본 발명의 조성물이 적용(처방)될 수 있는 대상은 포유동물 및 사람이며, 특히 사람인 경우가 바람직하다.Subjects to which the composition of the present invention can be applied (prescribed) are mammals and humans, particularly preferably humans.
본 발명의 조성물은 유효성분 이외에, 항염증 활성, 항알러지 활성 등 유사활성의 부가를 통한 복용이나 섭취의 편리성을 증진시키기 위하여, 당업계에서 이미 안전성이 검증되고 해당 활성을 갖는 것으로 공지된 임의의 화합물이나 천연 추출물을 추가로 포함할 수 있다.In order to enhance the convenience of taking or ingestion through addition of similar activities such as anti-inflammatory activity, anti-allergic activity, etc., in addition to the active ingredient, the composition of the present invention has already been verified for safety in the art and is known to have the corresponding activity. It may further contain a compound or natural extract of.
이러한 화합물 또는 추출물에는 각국 약전(한국에서는 "대한민국약전"), 각국 건강기능식품공전(한국에서는 식약처 고시인 "건강기능식품 기준 및 규격"임) 등의 공정서에 실려 있는 화합물 또는 추출물, 의약품의 제조·판매를 규율하는 각국의 법률(한국에서는 "약사법"임)에 따라 품목 허가를 받은 화합물 또는 추출물, 건강기능식품의 제조·판매를 규율하는 각국 법률(한국에서는 「건강기능식품에관한법률」임)에 따라 기능성이 인정된 화합물 또는 추출물이 포함될 수 있다.These compounds or extracts include compounds or extracts, pharmaceuticals, etc. listed in the pharmacopoeia of each country (“Korean Pharmacopoeia” in Korea), health functional food code in each country (“health functional food standards and standards” notified by the Ministry of Food and Drug Safety in Korea), etc. In accordance with the laws of each country governing the manufacture and sale of products (“Pharmaceutical Affairs Act” in Korea), laws governing the manufacture and sale of compounds, extracts, and health functional foods that have been licensed as items (in Korea, “The Act on Health Functional Foods”) ”), a compound or extract whose functionality is recognized may be included.
예컨대, 한국 「건강기능식품에관한법률」에 따라, 항염증("관절염 개선") 기능성이 인정된 MSM(dimethylsulfonylmethane), N-아세틸글루코사민 등과, 항알러지("과민 면역반응 완화") 기능성이 인정된 Enterococcus faecalis 가열 처리 건조 분말, 구아바 잎 추출물 등의 복합물, 다래 추출물, 소엽 추출물, 피카오프레토 분말 등의 복합물, PLAG(1-palmitoyl-2-linoleoyl-3-acetyl-rac-glycerol) 등이 이러한 화합물 또는 추출물에 해당할 것이며, 이에 특별히 제한되는 것은 아니다.For example, according to the Korean 「Health Functional Food Act」, anti-inflammatory ("arthritis improvement") functions are recognized, such as MSM (dimethylsulfonylmethane) and N-acetylglucosamine, and anti-allergic ("relieving hypersensitivity immune reactions") functions are recognized. Enterococcus faecalis heat-treated dry powder, complexes such as guava leaf extract, complexes such as vinegar extract, lobule extract, picaopreto powder, and PLAG (1-palmitoyl-2-linoleoyl-3-acetyl-rac-glycerol). It will correspond to the compound or extract, and is not particularly limited thereto.
이러한 화합물 또는 천연 추출물은 본 발명의 조성물에 그 유효성분과 함께 하나 이상 포함될 수 있다.One or more of these compounds or natural extracts may be included in the composition of the present invention together with the active ingredient.
본 발명의 조성물은 구체적인 양태에 있어서, 식품 조성물로서 파악할 수 있다. 또한, 본 발명의 식품에는 건강기능(성)식품이 포함될 수 있다. 본 발명에서 용어 "건강기능식품"이란, 인체에 유용한 기능성을 가진 원료나 성분을 사용하여 정제, 캅셀, 분말, 과립, 액상 및 환 등의 형태로 제조 및 가공한 식품을 말한다. 여기서 "기능성"이라 함은 인체의 구조 및 기능에 대하여 영양소를 조절하거나 생리학적 작용 등과 같은 보건용도에 유용한 효과를 얻는 것을 의미한다. 본 발명의 건강기능식품은 당업계에서 통상적으로 사용되는 방법에 의하여 제조 가능하며, 상기 제조시에는 당업계에서 통상적으로 첨가하는 원료 및 성분을 첨가하여 제조할 수 있다. 또한 상기 건강기능식품의 제형 또한 건강기능식품으로 인정되는 제형이면 제한 없이 제조될 수 있다. 본 발명의 식품 조성물은 다양한 형태의 제형으로 제조될 수 있으며, 일반 약품과는 달리 식품을 원료로 하여 약품의 장기 복용 시 발생할 수 있는 부작용 등이 없는 장점이 있고, 휴대성이 뛰어나, 본 발명의 건강기능식품은 COPD 개선 효과, 나아가 호흡기 기능 강화 및 호흡기 질환 개선 효과를 증진시키기 위한 보조제로 섭취가 가능하다.In a specific aspect, the composition of this invention can be grasped as a food composition. In addition, the food of the present invention may include health functional (sex) food. In the present invention, the term "health functional food" refers to a food manufactured and processed in the form of tablets, capsules, powders, granules, liquids and pills using raw materials or ingredients having useful functions for the human body. Here, the term "functionality" refers to obtaining useful effects for health purposes such as controlling nutrients or physiological effects on the structure and function of the human body. The health functional food of the present invention can be prepared by a method commonly used in the art, and at the time of production, it can be prepared by adding raw materials and ingredients commonly added in the art. In addition, the formulation of the health functional food may be prepared without limitation as long as it is a formulation recognized as a health functional food. The food composition of the present invention can be prepared in various forms of formulation, and unlike general drugs, it has the advantage of not having side effects that may occur when taking the drug for a long time using food as a raw material, and is excellent in portability. Health functional foods can be consumed as an adjuvant to improve COPD and further enhance respiratory function and improve respiratory disease.
본 발명의 식품 조성물은 어떠한 형태로도 제조될 수 있으며, 예컨대 차, 쥬스, 탄산음료, 이온음료 등의 음료류, 우유, 요구루트 등의 가공 유류(乳類), 껌류, 떡, 한과, 빵, 과자, 면 등의 식품류, 정제, 캡슐, 환, 과립, 액상, 분말, 편상, 페이스트상, 시럽, 겔, 젤리, 바 등의 건강기능식품 제제류 등으로 제조될 수 있다.The food composition of the present invention may be prepared in any form, such as beverages such as tea, juice, carbonated beverages, ionized beverages, processed oils such as milk, yogurt, gums, rice cakes, Korean confectionery, bread, Foods such as confectionery and noodles, tablets, capsules, pills, granules, liquids, powders, flakes, pastes, syrups, gels, jelly, and health functional food preparations such as bars can be prepared.
또 본 발명의 식품 조성물은 법률상·기능상의 구분에 있어서 제조·유통 시점의 시행 법규에 부합하는 한 임의의 제품 구분을 띨 수 있다. 예컨대 한국 「건강기능식품에관한법률」에 따른 건강기능식품이거나, 한국 「식품위생법」의 식품공전(식약처 고시 「식품의 기준 및 규격」)상 각 식품유형에 따른 과자류, 두류, 다류, 음료류, 특수용도식품 등일 수 있다.In addition, the food composition of the present invention can be classified as a product as long as it conforms to the enforcement regulations at the time of manufacture and distribution in terms of legal and functional classification. For example, it is a health functional food according to the Korean 「Health Functional Food Act」, or confectionery, bean, tea, and beverages according to each food type according to the food code of the Korean 「Food Sanitation Act」 (``Food Standards and Standards'' notified by the Ministry of Food and Drug Safety). , Special use food, etc.
본 발명의 식품 조성물에는 그 유효성분 이외에 식품첨가물이 포함될 수 있다. 식품첨가물은 일반적으로 식품을 제조, 가공 또는 보존함에 있어 식품에 첨가되어 혼합되거나 침윤되는 물질로서 이해될 수 있는데, 식품과 함께 매일 그리고 장기간 섭취되므로 그 안전성이 보장되어야 한다. 식품의 제조·유통을 규율하는 각국 법률(한국에서는 「식품위생법」임)에 따른 식품첨가물공전에는 안전성이 보장된 식품첨가물이 성분 면에서 또는 기능 면에서 한정적으로 규정되어 있다. 한국 식품첨가물공전(식약처 고시 「식품첨가물 기준 및 규격」)에서는 식품첨가물이 성분 면에서 화학적 합성품, 천연 첨가물 및 혼합 제제류로 구분되어 규정되어 있는데, 이러한 식품첨가물은 기능 면에 있어서는 감미제, 풍미제, 보존제, 유화제, 산미료, 점증제 등으로 구분된다.The food composition of the present invention may contain food additives in addition to the active ingredients. Food additives can generally be understood as substances that are added to food and mixed or infiltrated in manufacturing, processing, or preserving food. Since they are consumed daily and for a long time with food, their safety must be ensured. Food additives with guaranteed safety are limited in terms of ingredients or functions in the Food Additives Code according to the laws of each country governing the manufacture and distribution of food (the “Food Sanitation Act” in Korea). In the Korean Food Additives Code (“Food Additive Standards and Standards” notified by the Ministry of Food and Drug Safety), food additives are classified into chemical synthetic products, natural additives, and mixed preparations in terms of ingredients.These food additives are sweeteners and flavors in terms of function. It is categorized into agents, preservatives, emulsifiers, acidulants, and thickeners.
감미제는 식품에 적당한 단맛을 부여하기 위하여 사용되는 것으로, 천연의 것이거나 합성된 것을 사용할 수 있다. 바람직하게는 천연 감미제를 사용하는 경우인데, 천연 감미제로서는 옥수수 시럽 고형물, 꿀, 수크로오스, 프룩토오스, 락토오스, 말토오스 등의 당 감미제를 들 수 있다.Sweeteners are used to impart a suitable sweetness to food, and natural or synthetic ones may be used. Preferably, a natural sweetener is used, and examples of the natural sweetener include sugar sweeteners such as corn syrup solids, honey, sucrose, fructose, lactose, and maltose.
풍미제는 맛이나 향을 좋게 하기 위하여 사용될 수 있는데, 천연의 것과 합성된 것 모두 사용될 수 있다. 바람직하게는 천연의 것을 사용하는 경우이다. 천연의 것을 사용할 경우에 풍미 이외에 영양 강화의 목적도 병행할 수 있다. 천연 풍미제로서는 사과, 레몬, 감귤, 포도, 딸기, 복숭아 등에서 얻어진 것이거나 녹차잎, 둥굴레, 대잎, 계피, 국화 잎, 자스민 등에서 얻어진 것일 수 있다. 또 인삼(홍삼), 죽순, 알로에 베라, 은행 등에서 얻어진 것을 사용할 수 있다. 천연 풍미제는 액상의 농축액이나 고형상의 추출물일 수 있다. 경우에 따라서 합성 풍미제가 사용될 수 있는데, 합성 풍미제로서는 에스테르, 알콜, 알데하이드, 테르펜 등이 이용될 수 있다.Flavoring agents can be used to enhance taste or aroma, and both natural and synthetic can be used. Preferably, it is the case of using a natural one. In the case of using natural ones, the purpose of nutrient enhancement can be combined in addition to flavor. As a natural flavoring agent, it may be obtained from apples, lemons, tangerines, grapes, strawberries, peaches, or the like, or from green tea leaves, roundtails, bamboo leaves, cinnamon, chrysanthemum leaves, jasmine, and the like. In addition, you can use those obtained from ginseng (red ginseng), bamboo shoots, aloe vera, and ginkgo. The natural flavoring agent may be a liquid concentrate or a solid extract. In some cases, synthetic flavoring agents may be used. As synthetic flavoring agents, esters, alcohols, aldehydes, terpenes, and the like may be used.
보존제로서는 소르브산칼슘, 소르브산나트륨, 소르브산칼륨, 벤조산칼슘, 벤조산나트륨, 벤조산칼륨, EDTA(에틸렌디아민테트라아세트산) 등이 사용될 수 있고, 또 유화제로서는 아카시아검, 카르복시메틸셀룰로스, 잔탄검, 펙틴 등이 사용될 수 있으며, 산미료로서는 연산, 말산, 푸마르산, 아디프산, 인산, 글루콘산, 타르타르산, 아스코르브산, 아세트산, 인산 등이 사용될 수 있다. 산미료는 맛을 증진시키는 목적 이외에 미생물의 증식을 억제할 목적으로 식품 조성물이 적정 산도로 되도록 첨가될 수 있다.As a preservative, calcium sorbate, sodium sorbate, potassium sorbate, calcium benzoate, sodium benzoate, potassium benzoate, EDTA (ethylenediaminetetraacetic acid), etc. can be used, and as an emulsifier, acacia gum, carboxymethylcellulose, xanthan gum, pectin And the like may be used, and as the acidulant, arithmetic, malic acid, fumaric acid, adipic acid, phosphoric acid, gluconic acid, tartaric acid, ascorbic acid, acetic acid, phosphoric acid, and the like can be used. In addition to the purpose of enhancing taste, the acidulant may be added so that the food composition has an appropriate acidity for the purpose of inhibiting the growth of microorganisms.
점증제로서는 현탁화 구현제, 침강제, 겔형성제, 팽화제 등이 사용될 수 있다.As the thickening agent, a suspending agent, a settling agent, a gel-forming agent, a swelling agent, and the like may be used.
본 발명의 식품 조성물은 전술한 바의 식품첨가물 이외에, 기능성과 영양성을 보충, 보강할 목적으로 당업계에 공지되고 식품첨가물로서 안정성이 보장된 생리활성 물질이나 미네랄류를 포함할 수 있다.In addition to the food additives described above, the food composition of the present invention may include a physiologically active substance or minerals known in the art for the purpose of supplementing and reinforcing functionality and nutritional properties and ensuring stability as a food additive.
그러한 생리활성 물질로서는 녹차 등에 포함된 카테킨류, 비타민 B1, 비타민 C, 비타민 E, 비타민 B12 등의 비타민류, 토코페롤, 디벤조일티아민 등을 들 수 있으며, 미네랄류로서는 구연산칼슘 등의 칼슘 제제, 스테아린산마그네슘 등의 마그네슘 제제, 구연산철 등의 철 제제, 염화크롬, 요오드칼륨, 셀레늄, 게르마늄, 바나듐, 아연 등을 들 수 있다.Examples of such physiologically active substances include catechins contained in green tea, vitamins such as vitamin B1, vitamin C, vitamin E, and vitamin B12, tocopherol, dibenzoyl thiamine, etc., and minerals include calcium preparations such as calcium citrate, magnesium stearate. Magnesium preparations such as, iron preparations such as iron citrate, chromium chloride, potassium iodide, selenium, germanium, vanadium, and zinc.
본 발명의 식품 조성물에는 전술한 바의 식품첨가물이 제품 유형에 따라 그 첨가 목적을 달성할 수 있는 적량으로 포함될 수 있다.In the food composition of the present invention, the food additive described above may be included in an appropriate amount to achieve the purpose of addition according to the product type.
본 발명의 식품 조성물에 포함될 수 있는 기타의 식품첨가물과 관련하여서는 각국 식품공전이나 식품첨가물 공전을 참조할 수 있다.Regarding other food additives that may be included in the food composition of the present invention, reference may be made to the food code of each country or the food additive code.
본 발명의 조성물은 다른 구체적인 양태에 있어서는 약제학적 조성물로 파악될 수 있다.The composition of the present invention may be understood as a pharmaceutical composition in other specific embodiments.
본 발명의 약제학적 조성물은 유효성분 이외에 약제학적으로 허용되는 담체를 포함하여 당업계에 공지된 통상의 방법으로 투여 경로에 따라 경구용 제형 또는 비경구용 제형으로 제조될 수 있다. 여기서 투여 경로는 국소 경로, 경구 경로, 정맥 내 경로, 근육 내 경로, 및 점막 조직을 통한 직접 흡수를 포함하는 임의의 적절한 경로일 수 있으며, 두 가지 이상의 경로를 조합하여 사용할 수도 있다. 두 가지 이상 경로의 조합의 예는 투여 경로에 따른 두 가지 이상의 제형의 약물이 조합된 경우로서 예컨대 1차로 어느 한 약물은 정맥 내 경로로 투여하고 2차로 다른 약물은 국소 경로로 투여하는 경우이다.The pharmaceutical composition of the present invention may be prepared in an oral dosage form or a parenteral dosage form according to an administration route by a conventional method known in the art, including a pharmaceutically acceptable carrier in addition to the active ingredient. Here, the route of administration may be any suitable route including a local route, an oral route, an intravenous route, an intramuscular route, and direct absorption through mucosal tissue, and two or more routes may be used in combination. An example of a combination of two or more routes is a case in which two or more formulations of drugs are combined according to the route of administration, for example, when one drug is first administered by an intravenous route and the second drug is administered by a local route.
약학적으로 허용되는 담체는 투여 경로나 제형에 따라 당업계에 주지되어 있으며, 구체적으로는 "대한민국약전"을 포함한 각국의 약전을 참조할 수 있다.Pharmaceutically acceptable carriers are well known in the art depending on the route of administration or formulation, and specifically, reference may be made to the pharmacopoeia of each country, including the “Korean Pharmacopoeia”.
본 발명의 약제학적 조성물이 경구용 제형으로 제조될 경우, 적합한 담체와 함께 당업계에 공지된 방법에 따라 분말, 과립, 정제, 환제, 당의정제, 캡슐제, 액제, 겔제, 시럽제, 현탁액, 웨이퍼 등의 제형으로 제조될 수 있다. 이때 적합한 담체의 예로서는 락토스, 글루코스, 슈크로스, 덱스트로스, 솔비톨, 만니톨, 자일리톨 등의 당류, 옥수수 전분, 감자 전분, 밀 전분 등의 전분류, 셀룰로오스, 메틸셀룰로오스, 에틸셀룰로오스, 나트륨 카르복시메틸셀룰로오스, 하이드록시프로필메틸셀룰로오스 등의 셀룰로오스류, 폴리비닐 피롤리돈, 물, 메틸히드록시벤조에이트, 프로필히드록시벤조에이트, 마그네슘 스테아레이트, 광물유, 맥아, 젤라틴, 탈크, 폴리올, 식물성유, 에탄올, 그리세롤 등을 들 수 있다. 제제화활 경우 필요에 따라적절한 결합제, 윤활제, 붕해제, 착색제, 희석제 등을 포함시킬 수 있다. 적절한 결합제로서는 전분, 마그네슘 알루미늄 실리케이트, 전분페리스트, 젤라틴, 메틸셀룰로스, 소듐 카복시메틸셀룰로스, 폴리비닐피롤리돈, 글루코스, 옥수수 감미제, 소듐 알지네이트, 폴리에틸렌 글리콜, 왁스 등을 들 수 있고, 윤활제로서는 올레산나트륨, 스테아르산나트륨, 스테아르산마그네슘, 벤조산나트륨, 초산나트륨, 염화나트륨, 실리카, 탈쿰, 스테아르산, 그것의 마그네슘염과 칼슘염, 폴리데틸렌글리콜 등을 들 수 있으며, 붕해제로서는 전분, 메틸 셀룰로스, 아가(agar), 벤토나이트, 잔탄 검, 전분, 알긴산 또는 그것의 소듐 염 등을 들 수 있다. 또 희석제로서는 락토즈, 덱스트로즈, 수크로즈, 만니톨, 소비톨, 셀룰로스, 글라이신 등을 들 수 있다.When the pharmaceutical composition of the present invention is prepared in an oral dosage form, powders, granules, tablets, pills, dragees, capsules, solutions, gels, syrups, suspensions, wafers according to a method known in the art together with a suitable carrier It can be prepared in a formulation such as. Examples of suitable carriers at this time include sugars such as lactose, glucose, sucrose, dextrose, sorbitol, mannitol, and xylitol, starches such as corn starch, potato starch, wheat starch, cellulose, methylcellulose, ethylcellulose, sodium carboxymethylcellulose, Celluloses such as hydroxypropylmethylcellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, magnesium stearate, mineral oil, malt, gelatin, talc, polyol, vegetable oil, ethanol, grease Serol, etc. are mentioned. In the case of formulation, appropriate binders, lubricants, disintegrants, colorants, and diluents may be included as needed. Suitable binders include starch, magnesium aluminum silicate, starch ferryst, gelatin, methylcellulose, sodium carboxymethylcellulose, polyvinylpyrrolidone, glucose, corn sweetener, sodium alginate, polyethylene glycol, wax, etc., and lubricants include oleic acid. Sodium, sodium stearate, magnesium stearate, sodium benzoate, sodium acetate, sodium chloride, silica, talcum, stearic acid, magnesium salts and calcium salts thereof, polydecylene glycol, etc., and as disintegrating agents starch, methyl cellulose , Agar, bentonite, xanthan gum, starch, alginic acid, or a sodium salt thereof. Moreover, lactose, dextrose, sucrose, mannitol, sorbitol, cellulose, glycine, etc. are mentioned as a diluent.
본 발명의 약제학적 조성물이 비경구용 제형으로 제조될 경우, 적합한 담체와 함께 당업계에 공지된 방법에 따라 주사제, 경피 투여제, 비강 흡입제 및 좌제의 형태로 제제화될 수 있다. 주사제로 제제화할 경우 적합한 담체로서는 수성 등장 용액 또는 현탁액을 사용할 수 있으며, 구체적으로는 트리에탄올 아민이 함유된 PBS(phosphate buffered saline)나 주사용 멸균수, 5% 덱스트로스 같은 등장 용액 등을 사용할 수 있다. 경피 투여제로 제제화할 경우 연고제, 크림제, 로션제, 겔제, 외용액제, 파스타제, 리니멘트제, 에어롤제 등의 형태로 제제화할 수 있다. 비강 흡입제의 경우 디클로로플루오로메탄, 트리클로로플루오로메탄, 디클로로테트라플루오로에탄, 이산화탄소 등의 적합한 추진제를 사용하여 에어로졸 스프레이 형태로 제제화할 수 있으며, 좌제로 제제화할 경우 그 담체로는 위텝솔(witepsol), 트윈(tween) 61, 폴리에틸렌글리콜류, 카카오지, 라우린지, 폴리옥시에틸렌 소르비탄 지방산 에스테르류, 폴리옥시에틸렌 스테아레이트류, 소르비탄 지방산 에스테르류 등을 사용할 수 있다.When the pharmaceutical composition of the present invention is prepared in a parenteral formulation, it may be formulated in the form of an injection, a transdermal administration, a nasal inhalation and a suppository according to a method known in the art together with a suitable carrier. When formulated as an injection, an aqueous isotonic solution or suspension may be used as a suitable carrier, and specifically, triethanol amine-containing PBS (phosphate buffered saline), sterile water for injection, an isotonic solution such as 5% dextrose, etc. may be used. . When formulated as a transdermal administration, it can be formulated in the form of an ointment, cream, lotion, gel, external solution, pasta, liniment, air roll, and the like. In the case of nasal inhalants, suitable propellants such as dichlorofluoromethane, trichlorofluoromethane, dichlorotetrafluoroethane, and carbon dioxide can be used in the form of an aerosol spray.When formulated as a suppository, the carrier is Withepsol ( witepsol), tween 61, polyethylene glycols, cacao butter, laurin paper, polyoxyethylene sorbitan fatty acid esters, polyoxyethylene stearates, sorbitan fatty acid esters, and the like.
약제학적 조성물의 구체적인 제제화와 관련하여서는 당업계에 공지되어 있으며, 예컨대 문헌[Remington's Pharmaceutical Sciences(19th ed., 1995)] 등을 참조할 수 있다. 상기 문헌은 본 명세서의 일부로서 간주 된다.The specific formulation of pharmaceutical compositions is known in the art, and for example, Remington's Pharmaceutical Sciences (19th ed., 1995) may be referred to. This document is considered as part of this specification.
본 발명의 약제학적 조성물의 바람직한 투여량은 환자의 상태, 체중, 성별, 연령, 환자의 중증도, 투여 경로에 따라 1일 0.001mg/kg ~ 10g/kg 범위, 바람직하게는 0.001mg/kg ~ 1g/kg 범위일 수 있다. 투여는 1일 1회 또는 수회로 나누어 이루어질 수 있다. 이러한 투여량은 어떠한 측면으로든 본 발명의 범위를 제한하는 것으로 해석되어서는 아니 된다.The preferred dosage of the pharmaceutical composition of the present invention is in the range of 0.001 mg/kg to 10 g/kg per day, preferably 0.001 mg/kg to 1 g, depending on the patient's condition, weight, sex, age, patient severity, and route of administration. May be in the /kg range. Administration can be made once a day or divided into several times. Such dosage should not be construed as limiting the scope of the invention in any aspect.
전술한 바와 같이, 본 발명에 따르면 화살나무 추출물을 이용한 COPD 개선용 조성물을 제공할 수 있다. 본 발명의 조성물은 식품, 특히 건강기능식품 또는 약품으로 제품화되어, COPD 개선 효과를 위하여, 나아가 호흡기 기능 강화 및 호흡기 질환 개선 효과를 위하여 유용하게 사용될 수 있으며, 기침, 객담, 호흡 곤란, 기도 과민성, 기도 폐쇄, 점액 과분비 등의 증상을 수반하는 기타 폐질환 예컨대 미만성 간질성 폐질환, 급성호흡곤란증후군(acute respiratory distress syndrome, ARDS), 급성 폐손상 등의 개선 효과를 위하여서도 유용하게 사용될 수 있다.As described above, according to the present invention, it is possible to provide a composition for improving COPD using an arrow tree extract. The composition of the present invention is commercialized as a food, in particular a health functional food or drug, and can be usefully used for the effect of improving COPD, further enhancing respiratory function and improving respiratory diseases, coughing, sputum, shortness of breath, respiratory irritation, Other lung diseases accompanying symptoms such as airway obstruction and mucus hypersecretion, such as diffuse interstitial pulmonary disease, acute respiratory distress syndrome (ARDS), acute lung injury, etc. can also be usefully used for improvement.
도 1은 HL-60 세포에 대해 화살나무 추출물의 농도별 처리 시 Netosis 수준(%)을 평가한 결과를 나타낸 것이다.
도 2 및 도 3은 H292 세포에 대해 각각 화살나무 잎 또는 가지 추출물의 처리 시 IL-8 억제 활성을 평가한 결과를 나타낸 것이다.
도 4는 MH-S 세포에 대해 화살나무 추출물의 농도별 처리 시 MIP-2 억제 활성을 평가한 결과를 나타낸 것이다.
도 5는 PPE/CSE로 유도된 만성폐쇄성폐질환(또는 호흡기 질환)마우스 모델에 대해 화살나무 추출물을 투여한 후 기관지폐포세척액(BALF)로부터 계수된 총 세포수를 나타낸 것이다.
도 6 및 도 7은 Diff-Quick 염색을 통한 BALF 내 면역세포(Macrophage, Lympocyte, Neutrophils 및 Eosinophil)를 분석한 결과를 나타낸 것이다.
도 8은 마우스 모델로부터 적출된 폐 조직을 염색한 결과를 나타낸 것이다.
도 9는 BALF 내 Cytokine(TNF-alpha, MIP-2, IL-1beta, IL-6)을 분석한 결과를 나타낸 것이다.
도 10은 폐 조직으로부터 Cytokine(KC, MCP-1, MDC, TARC, GM-CSF, MIP-2, IL-6, TNF-alpha, IL-1beta, INF-gamma, IL-10 및 IL-17)을 분석한 결과를 나타낸 것이다.Figure 1 shows the results of evaluating the Netosis level (%) for HL-60 cells at the time of treatment by concentration of the arrow tree extract.
2 and 3 show the results of evaluating the IL-8 inhibitory activity upon treatment of the extracts of arrow tree leaves or branches for H292 cells, respectively.
4 shows the results of evaluating the MIP-2 inhibitory activity upon treatment of MH-S cells according to concentrations of the arrow tree extract.
Figure 5 shows the total number of cells counted from bronchoalveolar lavage fluid (BALF) after administration of the arrow tree extract to the PPE/CSE-induced chronic obstructive pulmonary disease (or respiratory disease) mouse model.
6 and 7 show the results of analyzing immune cells (Macrophage, Lympocyte, Neutrophils, and Eosinophil) in BALF through Diff-Quick staining.
8 shows the results of staining the lung tissue extracted from the mouse model.
9 shows the results of analysis of Cytokine (TNF-alpha, MIP-2, IL-1beta, IL-6) in BALF.
Figure 10 is Cytokine from lung tissue (KC, MCP-1, MDC, TARC, GM-CSF, MIP-2, IL-6, TNF-alpha, IL-1beta, INF-gamma, IL-10 and IL-17) It shows the results of analysis.
이하 본 발명을 실시예 및 실험예를 참조하여 설명한다. 그러나 본 발명의 범위가 이러한 실시예 및 실험예에 한정되는 것은 아니다.Hereinafter, the present invention will be described with reference to Examples and Experimental Examples. However, the scope of the present invention is not limited to these Examples and Experimental Examples.
[실시예] 시료 준비[Example] Sample preparation
화살나무(추출 부위: 잎, 가지)의 추출물을 제조하여 폐질환 개선 활성을 확인하였다. 추출물은 각 추출 대상 천연물의 건조 분말에 10배 중량의 70% 에탄올을 가하여 50℃에서 6시간씩 2회 반복 추출하여 여과한 후 감압농축 및 동결건조하여 분말상으로 얻었다.An extract of the arrow tree (extracted area: leaves, branches) was prepared to confirm the activity of improving lung disease. The extract was obtained by adding 10 times the weight of 70% ethanol to the dry powder of each natural product to be extracted, and repeatedly extracting it twice at 50°C for 6 hours, filtered, concentrated under reduced pressure, and freeze-dried to obtain a powder.
[실험예] 폐질환 개선 활성[Experimental Example] Lung disease improvement activity
실험예 1: 호중구 세포의 Netosis 억제 활성Experimental Example 1: Netosis inhibitory activity of neutrophil cells
HL-60 세포를 1% DMSO가 처리된 RPMI (10% FBS, 100 U/mL penicillin, 100 mg/mL streptomycin) 배지에 2×105 cell/mL로 seeding하고 세포배양기 (37℃, 5% CO2)에서 5일간 배양하여 mature neutrophil phenotype으로 분화시켰다. 5일후 1,300 rpm에서 5분간 원심분리 한 후, HBSS (with Mg+, Ca+)를 10 mL 처리하고 1,300 rpm에서 5분간 원심분리하여 세척하였다. 회수한 세포는 HBSS에 1×106 cell/mL 농도로 희석하여 96 well plate에 100 μL씩 분주하고 각각의 농도로 준비된 시료를 50 μL씩 처리하였다. 그 후 400 nM로 준비된 PMA 또는 CSE를 50 μL씩 각각의 well에 처리한 후 3시간 배양하였다. 3시간 후 Cytox green (500 nM)을 처리하고 형광 plate reader기를 이용하여 excitation/emission maxima: 485/520 nm에서 형광도를 측정하였다. HL-60 cells were seeded at 2×10 5 cells/mL in RPMI (10% FBS, 100 U/mL penicillin, 100 mg/mL streptomycin) medium treated with 1% DMSO, and a cell incubator (37°C, 5% CO). 2 ) was cultured for 5 days to differentiate into a mature neutrophil phenotype. After 5 days, after centrifugation at 1,300 rpm for 5 minutes, HBSS (with Mg +, Ca + ) was treated with 10 mL, followed by centrifugation at 1,300 rpm for 5 minutes to wash. The recovered cells were diluted in HBSS at a concentration of 1×10 6 cells/mL, dispensed into 96 well plates by 100 μL, and 50 μL of samples prepared at each concentration were treated. Then, 50 μL of PMA or CSE prepared at 400 nM was treated in each well and incubated for 3 hours. After 3 hours, Cytox green (500 nM) was treated and fluorescence was measured at excitation/emission maxima: 485/520 nm using a fluorescent plate reader.
그 결과, Netosis 수준(%)은 처리된 화살나무 잎 추출물의 농도(12.5 ㎍/ml, 25 ㎍/ml, 50 ㎍/ml, 100 ㎍/ml) 의존적으로 감소되는 것을 확인하였다(도 1).As a result, it was confirmed that the level of Netosis (%) decreased depending on the concentration (12.5 μg/ml, 25 μg/ml, 50 μg/ml, 100 μg/ml) of the treated arrowhead leaf extract (FIG. 1).
실험예 2: H292 세포를 이용한 염증성 사이토카인(IL-8) 억제활성 평가Experimental Example 2: Evaluation of inflammatory cytokine (IL-8) inhibitory activity using H292 cells
24 well plate에 2×105 cells/mL 농도로 준비한 H292 세포를 500 μL/well로 계대하여 배양한 후 세포가 80% 이상 confluent 되었을 때, serum-free RPMI1640 배지로 교환하여 24시간 starvation 하였다. 그 후 Serum-free RPMI1640 배지를 이용하여 자극물질 (CSE; 최종 2% 또는 PPE; 최종 0.01U/mL 또는 PM2.5; 최종 10 μg/mL)과 화살나무 추출물(최종; 6.25, 12.5, 25 μg/mL)을 원하는 최종농도를 반영하여 희석한 후, well당 500 uL씩 처리하였다. 하룻밤 배양 후 상징액을 회수하여 IL-8 측정에 사용하였다. Human IL-8은 ELISA법으로 측정하였으며, 제조사(BD社)의 프로토콜에 따라 진행하였다. H292 cells prepared at a concentration of 2×10 5 cells/mL in a 24 well plate were passaged and cultured at 500 μL/well, and when the cells became more than 80% confluent, they were exchanged with serum-free RPMI1640 medium and starvated for 24 hours. Thereafter, using Serum-free RPMI1640 medium, irritants (CSE; final 2% or PPE; final 0.01 U/mL or PM2.5; final 10 μg/mL) and arrowhead extract (final; 6.25, 12.5, 25 μg) /mL) was diluted to reflect the desired final concentration, and then treated at 500 uL per well. After incubation overnight, the supernatant was recovered and used for IL-8 measurement. Human IL-8 was measured by the ELISA method, and proceeded according to the manufacturer's (BD) protocol.
그 결과, IL-8 농도는 처리된 화살나무 잎 추출물의 농도(6.25 ㎍/ml, 12.5 ㎍/ml, 25 ㎍/ml) 의존적으로 억제되며, 화살나무 가지 추출물을 처리(6.25 ㎍/ml)한 경우에도 IL-8이 억제되는 것을 확인하였다(도 2 및 도 3).As a result, the concentration of IL-8 was inhibited depending on the concentration (6.25 µg/ml, 12.5 µg/ml, 25 µg/ml) of the treated arrow tree leaf extract, and treated with the arrow tree branch extract (6.25 µg/ml). Even in the case, it was confirmed that IL-8 was inhibited (FIGS. 2 and 3).
실험예 3: MH-S 세포를 이용한 MIP-2 억제활성 평가Experimental Example 3: Evaluation of MIP-2 inhibitory activity using MH-S cells
MH-S cell line은 ATCC 사에서 구매하였으며, 배양에 사용한 배지 조성은 RMPI-1640 (WELGENE, cat. LM 011-01), 10% FBS (WELGENE, cat. S 001-07), 1% Penicillin-Streptomycin (WELGENE, cat. LS 202-02), 14.3 mM 2-Mercptoethanol (SIGMA)였다. MH-S 세포를 5x104/well 농도로 cell culture 96 well plate (SPL, cat. 30096)에 seeding 하여 24시간 배양 하였다. 그 후 MH-S 세포 배양 배지로 자극물질 (CSE; 최종 1% 및 LPS; 최종 10 ng/mL )과 화살나무 잎 추출물(최종; 25 μg/mL)을 원하는 최종농도를 반영하여 희석하고 well당 250 uL씩 처리하였다. 24 시간 배양 후, 상징액을 회수하여 MIP-2 측정에 사용하였다. MIP-2는 ELISA법으로 측정하였으며, 제조사(R&D, DY452)의 프로토콜에 따라 진행하였다 The MH-S cell line was purchased from ATCC, and the medium composition used for cultivation was RMPI-1640 (WELGENE, cat. LM 011-01), 10% FBS (WELGENE, cat. S 001-07), 1% Penicillin- Streptomycin (WELGENE, cat. LS 202-02), 14.3 mM 2-Mercptoethanol (SIGMA). MH-S cells were seeded in a cell culture 96 well plate (SPL, cat. 30096) at a concentration of 5x10 4 /well and cultured for 24 hours. After that, dilute the stimulant (CSE; final 1% and LPS; final 10 ng/mL) and arrowhead leaf extract (final; 25 μg/mL) with MH-S cell culture medium to reflect the desired final concentration and dilute per well. Treated with 250 uL each. After incubation for 24 hours, the supernatant was recovered and used for MIP-2 measurement. MIP-2 was measured by the ELISA method, and proceeded according to the manufacturer's (R&D, DY452) protocol.
그 결과, 화살나무 잎 추출물 처리(25 ㎍/ml) 시 MIP-2 억제 활성을 나타내는 것을 확인하였다(도 4).As a result, it was confirmed that MIP-2 inhibitory activity was exhibited upon treatment with an arrow tree leaf extract (25 μg/ml) (FIG. 4).
실험예 4: 동물 모델에서 BALF 세포 수 측정Experimental Example 4: Measurement of the number of BALF cells in an animal model
4-1: PPE+CSE-유도된 호흡기 질환 마우스 모델4-1: PPE+CSE-induced respiratory disease mouse model
오리엔트 바이오사에서 BALB/C 마우스(male, 5주령)를 구입하여 1주일간 순화하였으며, n=10로 group화 하였다. 샘플 처리군은 COPD 유도 시작 일주일 전부터 해부전까지 24일간 200 mg/kg의 샘플(화살나무 추출물)을 경구투여하였으며, Naive와 COPD 군은 동량의 PBS를 투여하였다. 이 때 Positive control인 Roflumilast 군은 유도 시작 후 매일 10 mg/kg 농로 경구투여 하였다. COPD 유도에는 Porcine Pancreatic Elastase (PPE) 1.2 unit과 100% Cigarette Smoke Extraction (CSE)를 사용하였다. PPE 1.2 unit은 7일에 한번씩 intra nasal을 통해 총 3회 투입하였으며, CSE는 PPE 처리 다음날부터 3일간 intra nasal을 통해 20 ㎕ 처리하되, 마지막 PPE 처리 후에는 CSE를 처리하지 않고 다음 날 해부를 진행하였다. 해부는 흡입 마취기를 이용하여 isoflurane으로 마취시킨 후 진행하였다. 실험기간 동안 사료와 음용수는 자율급식 형태로 제공하였으며, 24 ℃, 습도 60% 환경에서 사육하였다.BALB/C mice (male, 5 weeks old) were purchased from Orient Bio, and purified for 1 week, and grouped with n=10. The sample treatment group was orally administered 200 mg/kg of the sample (arrow tree extract) for 24 days from one week before the start of COPD induction to pre-dissection, and the Naive and COPD groups were administered the same amount of PBS. At this time, the positive control, Roflumilast group, was administered orally at 10 mg/kg per day after induction. For COPD induction, 1.2 units of Porcine Pancreatic Elastase (PPE) and 100% Cigarette Smoke Extraction (CSE) were used. 1.2 unit of PPE was injected three times through intra nasal once every 7 days, and 20 µl of CSE was treated through intra nasal for 3 days from the day after PPE treatment, but after the last PPE treatment, the dissection proceeded the next day without CSE treatment. I did. The dissection was performed after anesthetizing with isoflurane using an inhalation anesthesia. During the experiment, feed and drinking water were provided in the form of self-catering, and were raised in an environment of 24 ℃ and 60% humidity.
4-2: BALF 내 침투된 총 세포수4-2: Total number of cells infiltrated into BALF
마우스의 기도를 통해 PBS 1 mL을 주입한 후 가볍게 마사지하여 700 μL의 기관지폐포세척액 (bronchoalveolar lavage fluid; BALF)을 회수하였다. 회수한 BALF액 10 uL를 Accustain T solution과 동량 혼합하여 Accuchip channel에 12 uL 주입 한 후 cell counter (ADAM-MC, NANOENTEK. INC, Korea)로 total cell을 자동 계수하였다(도 5).After injecting 1 mL of PBS through the airway of the mouse, it was gently massaged to recover 700 μL of bronchoalveolar lavage fluid (BALF). 10 uL of the recovered BALF solution was mixed with the Accustain T solution in the same amount, and 12 uL was injected into the Accuchip channel, and then total cells were automatically counted with a cell counter (ADAM-MC, NANOENTEK. INC, Korea) (FIG. 5).
4-3: Diff-Quick 염색을 통한 BALF 내 면역세포 분석4-3: Analysis of immune cells in BALF through Diff-Quick staining
회수한 BALF는 300×g, 5분 조건에서 원심분리를 통해 상층액과 세포 pellet으로 분리하였다. cell pellet은 PBS 700 μL를 첨가하여 재현탁하였으며, 이 BALF 현탁액 150 μL를 Cytospin device(Centrifuge 5403, Eppendorf, Hamburg, Germany)로 1000 rpm, 10 min, 4℃ 조건에서 원심분리하여 slide에 BALF 세포를 부착시켰다. 이 후 Diff-Quick staining reagent를 사용하여 제조사 (1-5-1 Wakinohamakaigandori, chuo-ku, Kobe, Japan)의 프로토콜에 준하여 세포를 염색한 후 현미경 관찰을 통해 Macrophages, Lympocytes, Neutrophils, Eosinophils의 수를 계수하였다(도 6, 도 7).The recovered BALF was separated into a supernatant and a cell pellet through centrifugation under conditions of 300×g and 5 minutes. The cell pellet was resuspended by adding 700 μL of PBS, and 150 μL of this BALF suspension was centrifuged at 1000 rpm, 10 min, 4°C with a Cytospin device (Centrifuge 5403, Eppendorf, Hamburg, Germany), and the BALF cells were placed on the slide. Attached. Thereafter, cells were stained according to the manufacturer's protocol (1-5-1 Wakinohamakaigandori, chuo-ku, Kobe, Japan) using a Diff-Quick staining reagent, and the number of Macrophages, Lympocytes, Neutrophils, and Eosinophils were determined through microscopic observation. Counted (Fig. 6, Fig. 7).
4-4: 조직 분석 (Histological examination)4-4: Histological examination
해부한 마우스로부터 폐 조직을 적출하여 폐의 중간엽을 제거한 후 10% neutral buffered formalin에 3일간 고정하였다. 고정한 조직을 ethyl alcohol과 xylene으로 탈수 시킨 후 파라핀으로 포매 한 후 삭정하고 5 um 두께로 박절하였다. 준비한 절편을 슬라이드에 부착시킨 후, 일반적인 형태를 확인하기 위해 H&E로 염색한 후 현미경으로 관찰하였다(도 8).Lung tissue was removed from the dissected mouse, the mesenchyme of the lung was removed, and then fixed in 10% neutral buffered formalin for 3 days. The fixed tissue was dehydrated with ethyl alcohol and xylene, embedded with paraffin, trimmed, and cut into 5 um thickness. After attaching the prepared section to the slide, it was stained with H&E to confirm the general shape and observed under a microscope (FIG. 8).
4-5: BALF 내 Cytokines 분석4-5: Analysis of Cytokines in BALF
마우스의 기도를 통해 PBS 1 mL을 주입한 후 가볍게 마사지하여 700 μL의 기관지폐포세척액 (bronchoalveolar lavage fluid; BALF)을 회수하였다. 회수한 BALF는 300×g, 5분 조건에서 원심분리하여 상층액을 분리 한 후 cytokines 및 chemokines 분석(TARC, KC, MCP-1, MDC, GM-CSF, MIP-2, TNF-α, IL-6, IL-1β, IL-17, IL-10, IFN-γ)에 사용하였다. cytokines 및 chemokines 분석을 위해 상층액 30 uL를 취한 후 Q-plex ELISA array kit의 제조사(Quansis biosciences 社) 프로토콜에 따라 실험을 진행하였다. 그 결과는 도 9에 각각의 cytokine 별로 나타내었다.After injecting 1 mL of PBS through the airway of the mouse, it was gently massaged to recover 700 μL of bronchoalveolar lavage fluid (BALF). The recovered BALF was centrifuged at 300×g for 5 minutes to separate the supernatant, and then analyzed for cytokines and chemokines (TARC, KC, MCP-1, MDC, GM-CSF, MIP-2, TNF-α, IL- 6, IL-1β, IL-17, IL-10, IFN-γ). After taking 30 uL of the supernatant for analysis of cytokines and chemokines, the experiment was performed according to the protocol of the manufacturer of the Q-plex ELISA array kit (Quansis biosciences). The results are shown for each cytokine in FIG. 9.
4-6: 폐 조직의 Cytokines 분석4-6: Analysis of Cytokines in Lung Tissue
폐 조직 무게의 10배에 해당하는 PBS를 첨가하고 homogenizer로 분쇄한 후 12,000 rpm, 20 min 조건에서 원심분리하였다. 회수한 상등액은 cytokines 및 chemokines 분석 (TARC, KC, MCP-1, MDC, GM-CSF, MIP-2, TNF-α, IL-6, IL-1β, IL-17, IL-10, IFN-γ)에 사용하였다. cytokines 및 chemokines 분석을 위해 상층액 30 uL를 취한 후 Q-plex ELISA array kit의 제조사(Quansis biosciences 社) 프로토콜에 따라 실험을 진행하였다. 그 결과는 도 10에 각각 나타내었다. PBS corresponding to 10 times the weight of the lung tissue was added, pulverized with a homogenizer, and centrifuged at 12,000 rpm for 20 min. The recovered supernatant was analyzed for cytokines and chemokines (TARC, KC, MCP-1, MDC, GM-CSF, MIP-2, TNF-α, IL-6, IL-1β, IL-17, IL-10, IFN-γ ). After taking 30 uL of the supernatant for analysis of cytokines and chemokines, the experiment was performed according to the protocol of the manufacturer of the Q-plex ELISA array kit (Quansis biosciences). The results are shown in Fig. 10, respectively.
이상의 설명으로부터, 본 발명이 속하는 기술분야의 당업자는 본 발명이 그 기술적 사상이나 필수적 특징을 변경하지 않고서 다른 구체적인 형태로 실시될 수 있다는 것을 이해할 수 있을 것이다. 이와 관련하여, 이상에서 기술한 실시예들은 모든 면에서 예시적인 것이며 한정적인 것이 아닌 것으로 이해해야만 한다. 본 발명의 범위는 상기 상세한 설명보다는 후술하는 특허 청구범위의 의미 및 범위 그리고 그 등가 개념으로부터 도출되는 모든 변경 또는 변형된 형태가 본 발명의 범위에 포함되는 것으로 해석되어야 한다.From the above description, those skilled in the art to which the present invention pertains will understand that the present invention can be implemented in other specific forms without changing the technical spirit or essential features thereof. In this regard, it should be understood that the embodiments described above are illustrative in all respects and not limiting. The scope of the present invention should be construed as including the meaning and scope of the claims to be described later rather than the above detailed description, and all changes or modifications derived from the equivalent concepts within the scope of the present invention.
Claims (10)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020180105190 | 2018-09-04 | ||
KR20180105190 | 2018-09-04 |
Publications (2)
Publication Number | Publication Date |
---|---|
KR20200027455A KR20200027455A (en) | 2020-03-12 |
KR102247364B1 true KR102247364B1 (en) | 2021-05-04 |
Family
ID=69803265
Family Applications (3)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1020190109804A KR102247367B1 (en) | 2018-09-04 | 2019-09-04 | Composition for improving respiratory diseases using the extract of Chaenomeles sinensis |
KR1020190109803A KR102247364B1 (en) | 2018-09-04 | 2019-09-04 | Composition for improving respiratory diseases using the extract of Euonymus alatus |
KR1020190109799A KR102304263B1 (en) | 2018-09-04 | 2019-09-04 | Composition for improving respiratory diseases using the extract of Camellia sinensis |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1020190109804A KR102247367B1 (en) | 2018-09-04 | 2019-09-04 | Composition for improving respiratory diseases using the extract of Chaenomeles sinensis |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1020190109799A KR102304263B1 (en) | 2018-09-04 | 2019-09-04 | Composition for improving respiratory diseases using the extract of Camellia sinensis |
Country Status (1)
Country | Link |
---|---|
KR (3) | KR102247367B1 (en) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20230098006A1 (en) | 2020-03-04 | 2023-03-30 | Hyundai Motor Company | Method and System for Collecting and Managing Vehicle-Generated Data |
KR102470820B1 (en) | 2021-11-30 | 2022-11-28 | 전북대학교산학협력단 | Composition for Improving Lung Injury comprising Salt Marsh Plants as an active ingredient |
KR20240033710A (en) | 2022-09-02 | 2024-03-13 | (주)에스디생명공학 | Composition for Prevention and Treatment of Cough, Sputum, and Bronchial Disease as an Active Ingredient |
KR20240087242A (en) | 2022-12-12 | 2024-06-19 | 양정은 | Composition for Prevention or Treatment Respiratory Diseases Comprising Combination Extracts of Sorbus commixta, Hibiscus syriacus and Ulmus macrocarpa as an Active Ingredient |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR100501831B1 (en) | 2003-05-12 | 2005-07-20 | 한국생명공학연구원 | Antiviral agent inhibiting the cytopathic effect of coronaviruses |
CN106581598A (en) | 2015-10-16 | 2017-04-26 | 张瑞芹 | Asthma treatment traditional Chinese medicine prescription |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR100674603B1 (en) * | 2005-05-10 | 2007-01-25 | (주)토종물산 | Health beverage composition comprising an extract of Platycodon grandiflorum, Glycyrrhiza uralensis, Liriope platyphylla and Chaenomeles sinesis koehne for preventing a respiratory disease |
KR101136090B1 (en) * | 2009-12-24 | 2012-04-17 | 정세헌 | A functional food and making method of thereof |
KR101160743B1 (en) * | 2010-02-18 | 2012-06-28 | 연세대학교 산학협력단 | Anti-viral agent against avian influenza virus comprising green tea |
KR101261888B1 (en) * | 2010-10-11 | 2013-05-07 | 정세헌 | A manufacturing method of foods for natural drugstuff for there by natural drugstuff |
KR20140043610A (en) * | 2012-10-02 | 2014-04-10 | 대전대학교 산학협력단 | Extracts of chaenomeles sinensis for the treatment or prevention of hypersensitivity immune disease and pharmaceutical compositions for hypersensitivity immune disease comprising the extracts |
KR20140092947A (en) * | 2012-12-31 | 2014-07-25 | 한림대학교 산학협력단 | The composition for allergic asthma mitigation extracted from tea seed |
KR20150083327A (en) * | 2014-01-09 | 2015-07-17 | 한국생명공학연구원 | Composition comprising an extract or a fraction of Euonymus alatus for preventing or treating asthma |
KR20170092733A (en) * | 2016-02-03 | 2017-08-14 | 김동명 | A composition of oriental medical herbs for prevention and/or improving of respiratory diseases |
KR20180010427A (en) * | 2016-07-21 | 2018-01-31 | 주식회사 벤스랩 | Plant complex extract having improved effect to allergic rhinitis, atopic dermatitis or chronic asthma |
KR101970320B1 (en) * | 2017-06-23 | 2019-04-18 | 대구가톨릭대학교산학협력단 | Composition for preventing, improving or treating respiratory diseases comprising fermented solution of Chaenomeles sinensis as effective component and production method thereof |
-
2019
- 2019-09-04 KR KR1020190109804A patent/KR102247367B1/en active IP Right Grant
- 2019-09-04 KR KR1020190109803A patent/KR102247364B1/en active IP Right Grant
- 2019-09-04 KR KR1020190109799A patent/KR102304263B1/en active IP Right Grant
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR100501831B1 (en) | 2003-05-12 | 2005-07-20 | 한국생명공학연구원 | Antiviral agent inhibiting the cytopathic effect of coronaviruses |
CN106581598A (en) | 2015-10-16 | 2017-04-26 | 张瑞芹 | Asthma treatment traditional Chinese medicine prescription |
Also Published As
Publication number | Publication date |
---|---|
KR20200027451A (en) | 2020-03-12 |
KR102304263B1 (en) | 2021-09-24 |
KR20200027455A (en) | 2020-03-12 |
KR20200027456A (en) | 2020-03-12 |
KR102247367B1 (en) | 2021-05-04 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
KR102247364B1 (en) | Composition for improving respiratory diseases using the extract of Euonymus alatus | |
KR102646341B1 (en) | Composition for improving respiratory disease comprising Artemisia capillaris extract as an active ingredient | |
KR20190133637A (en) | Composition for improving chronic obstructive pulmonary disease using an extract of Bark of Poria cocos | |
KR102252124B1 (en) | Composition for improving respiratory diseases using the extract of Picrasma quassioides | |
KR102467856B1 (en) | Composition for improving respiratory disease comprising Epilobium angustifolium extract as an active ingredient | |
KR102302910B1 (en) | Composition for improving respiratory diseases using the extract of Rosa davurica | |
KR102254572B1 (en) | Composition for improving chronic obstructive pulmonary disease using an extract of Elsholtzia ciliate | |
KR102273066B1 (en) | Composition for improving chronic obstructive pulmonary disease using an extract of Podocarpus macrophyllus | |
KR102346724B1 (en) | Composition for improving asthma or chronic obstructive pulmonary disease using an extract of Epilobium pyrricholophum | |
KR102434641B1 (en) | Food composition for improving respiratory function using Pediococcus pentosaceus | |
KR20200027453A (en) | Composition for improving respiratory diseases using the extract of Reynoutria elliptica | |
WO2021071292A2 (en) | Composition, using lactobacillus plantarum strain or like, for alleviation of respiratory diseases | |
KR102328744B1 (en) | Composition for improving respiratory disease comprising Lactobacillus plantarum strain as an active ingredient | |
EP3838282A1 (en) | Composition for alleviating asthma or chronic obstructive pulmonary disease by using epilobium pyrricholophum extract and like | |
KR102256880B1 (en) | Composition for improving respiratory diseases using the extract of Paliurus ramosissimus | |
KR20200135640A (en) | Composition for improving asthma or chronic obstructive pulmonary disease using an extract of Curcuma longa, Scutellariae Radix, etc | |
KR102245814B1 (en) | Composition for Improving Respiratory Disease Using an Extract of Sargassum horneri | |
KR102273075B1 (en) | Composition for improving respiratory diseases using the extract of Chrysosplenium grayanum | |
EP3848043A1 (en) | Composition for improving respiratory disease including extract of paliurus ramosissimus (lour.) poir | |
KR20210143967A (en) | Composition for preventing, improving or treating respiratory disease | |
KR20200134819A (en) | Composition for improving asthma or chronic obstructive pulmonary disease using an extract of Ficus religiosa, etc | |
KR20210047813A (en) | Composition for improving respiratory disease comprising an extract of Hypericum ascyron, etc | |
KR20220047193A (en) | Composition for antitussive or expectorant comprising Artemisia gmelinii extract | |
KR20230114649A (en) | Composition for immunity stimulatory activity Using an Extract of Astilbe rubra |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A201 | Request for examination | ||
E902 | Notification of reason for refusal | ||
E701 | Decision to grant or registration of patent right | ||
GRNT | Written decision to grant |