KR102183227B1 - New strawberry variety NSR090201 having anthracnose resistance and breeding method thereof - Google Patents

New strawberry variety NSR090201 having anthracnose resistance and breeding method thereof Download PDF

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KR102183227B1
KR102183227B1 KR1020180158744A KR20180158744A KR102183227B1 KR 102183227 B1 KR102183227 B1 KR 102183227B1 KR 1020180158744 A KR1020180158744 A KR 1020180158744A KR 20180158744 A KR20180158744 A KR 20180158744A KR 102183227 B1 KR102183227 B1 KR 102183227B1
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김혜란
이정여
노영희
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한국생명공학연구원
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    • A—HUMAN NECESSITIES
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    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
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    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
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    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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    • A23L21/00—Marmalades, jams, jellies or the like; Products from apiculture; Preparation or treatment thereof
    • A23L21/10—Marmalades; Jams; Jellies; Other similar fruit or vegetable compositions; Simulated fruit products

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Abstract

본 발명은 탄저병 저항성 딸기 신품종 NSR090201 및 이의 육종 방법에 관한 것으로, 딸기 신품종 NSR090201은 반촉성 적응성으로 탄저병 저항계이고, 초형은 반개장형이며 1화방의 개화기는 모본인 아키히메보다 한달 늦으며, 과실경도는 중간 정도이고, 당도는 11.6°Brix이며, 시들음병 및 흰가루병에도 강한 특성이 있다.The present invention relates to a new anthrax-resistant strawberry variety NSR090201 and a breeding method thereof, and the new strawberry variety NSR090201 is an anthrax-resistance meter with semi-touch adaptability, the super-type is semi-opened, and the flowering period of one flower room is one month later than the parent Akihime, and the fruit hardness is medium. It is about, and its sugar content is 11.6°Brix, and it has strong characteristics against wilt and powdery mildew.

Description

탄저병 저항성 딸기 신품종 NSR090201 및 이의 육종 방법{New strawberry variety NSR090201 having anthracnose resistance and breeding method thereof}New strawberry variety NSR090201 having anthracnose resistance and breeding method thereof

본 발명은 탄저병 저항성 딸기 신품종 NSR090201 및 이의 육종 방법에 관한 것이다.The present invention relates to an anthrax-resistant strawberry new variety NSR090201 and a breeding method thereof.

딸기(Fragaria x ananassa Duch.)는 쌍떡잎 식물의 장미과 딸기속에 속하는 열매채소로, 우리나라는 '설향', 일본은 '아키히메', '레드펄' 품종이 많이 재배되고 있다. 딸기는 묘를 길러 수확하기까지 거의 1년 내내 재배되는 작물로, 육묘기에는 탄저병과 시들음병, 과일 수확기에는 흰가루병과 잿빛곰팡이병이 많이 발생한다.Strawberry ( Fragaria x ananassa Duch.) is a fruit vegetable belonging to the strawberry genus of the dicotyledonous plant, and many varieties of'Solhyang' in Korea,'Akihime' in Japan, and'Red Pearl' are grown. Strawberries are grown almost all year round from growing seedlings to harvest, and anthrax and wilt disease are common during the seedling period, and powdery mildew and gray mold disease are common during the fruit harvesting period.

딸기의 관부, 잎자루, 런너(포복경)에 주로 발생하는 탄저병(anthracnose)은 콜레토트리쿰 프럭티콜라 (Colletotrichum fructicola)에 의해 발병하며, 고온 다습한 여름철에 많이 발생한다. 런너, 잎, 잎자루 등에 흑갈색의 반점이 생겨서 다습한 날씨가 계속되면 병반이 확대되고 썩어 들어가며, 런너를 통해 관부까지 병균이 침투되면 포기 전체가 시들어 죽게 되어 경제적 피해가 심하다. 우리나라에서 현재 딸기 탄저병 방제 전용으로 사용 중인 약제가 없는 실정이다. 최근 딸기에 발생하는 병해충을 친환경으로 방제하기 위한 유기농업자재나 천적과 같은 친환경자재에 관한 연구들이 진행되고 있지만, 탄저병이 발병하는 육묘기에 적용 가능한 방제력 개발은 미미한 실정으로 딸기 탄저병을 확실하게 예방하고 방제할 수 있는 효과적인 방법은, 탄저병 저항성 품종을 육성하는 것이다.Anthracnose, which occurs mainly in the tube, petiole, and runner (crawler) of strawberries, is caused by Colletotrichum fructicola , and occurs frequently in hot and humid summer. As dark brown spots appear on runners, leaves, petioles, etc., if humid weather continues, the lesions expand and rot, and when pathogens penetrate into the tube through the runner, the entire abandonment withers and dies, causing serious economic damage. Currently, there are no drugs currently used for controlling strawberry anthrax in Korea. Recently, researches on organic materials such as organic farming materials and natural enemies for environmentally friendly control of pests that occur in strawberries are in progress, but development of control power applicable to the breeding stage of anthrax outbreak is insignificant. An effective way to control is to cultivate anthrax resistant varieties.

한편, 한국공개특허 제2018-0117268호에는 설향을 모본으로 하고 매향을 부본으로 하여 육종한 '신품종 딸기 메리퀸 및 이의 육종 방법'이 개시되어 있고, 한국등록특허 제1556656호에는 아키히메 품종을 모본으로 하고 매향 품종을 부본으로 하여 육종한 '신품종 딸기 담향 및 이의 육종 방법'이 개시되어 있으나, 본 발명의 탄저병 저항성 딸기 신품종 NSR090201 및 이의 육종 방법에 대해서는 기재된 바가 없다.On the other hand, Korean Patent Laid-Open Patent No. 2018-0117268 discloses a'new variety strawberry mariquin and its breeding method', which was bred using Seolhyang as a model and Maeyang as a counterpart, and Korean Patent No. 1556656 discloses Akihime varieties. The'new variety of strawberry damhyang and its breeding method', which was bred using the Maehyang variety as a counterpart, is disclosed, but there is no description of the new variety of anthrax resistant strawberry NSR090201 and its breeding method of the present invention.

본 발명은 상기와 같은 요구에 의해 도출된 것으로서, 본 발명자들은 탄저병 저항성 계통 육성을 위해서 '아키히메' 품종을 모본으로 '수홍' 품종을 부본으로 이용하여 교배조합을 작성하였고, 탄저병 저항성 개체를 선발한 후 생산력 검정시험을 진행하였다. 탄저병 저항성 개체로 선발된 NSR090201 품종은 초형은 반개장형으로 초세가 약하고, 과형은 짧은 원추형이며, 초세에 비해 꽃 수가 많으므로 꽃 솎음이 필요하지만, 엽병장 및 엽장이 짧고, 연속출뢰성이 우수한 특성을 보였다. 또한, 탄저병을 비롯하여 시들음병, 흰가루병에 저항성을 갖는 특성을 보였으며, 과실 경도는 248g/∮5mm로 중간 정도로 단단하고, 당도는 11.6°Brix로 맛이 우수한 특성을 보이는 것을 확인함으로써, 본 발명을 완성하였다.The present invention was derived from the above requirements, and the present inventors created a crossing association using the'Akihime' variety as a model and the'Suhong' variety as a counterpart to foster anthrax resistant strains, and selected anthrax resistant individuals After that, a productivity test was conducted. The NSR090201 cultivar selected as an anthrax-resistant individual is semi-opened, has a weak superfine, and has a short conical shape, and requires thinning of flowers because there are more flowers than the superfine. Showed. In addition, it showed resistance to anthracnose, wilt disease, and powdery mildew, fruit hardness of 248g/∮5mm, which is moderately hard, and sugar content of 11.6°Brix, which shows excellent taste characteristics, thereby completing the present invention. I did.

상기 과제를 해결하기 위해, 본 발명은 아키히메 품종을 모본으로 하고, 수홍 품종을 부본으로 하여 이를 교배시켜 얻어진 것으로서, 탄저병, 시들음병 및 흰가루병에 강하고, 당도는 11~12°Brix이며, 연속출뢰성이 우수한 특성을 가지는, 기탁번호가 KCTC18720P인 딸기 신품종 NSR090201을 제공한다.In order to solve the above problem, the present invention is obtained by crossing the Akihime variety as a parent, and the scarlet variety as a counterpart, and is resistant to anthrax, wilt and powdery mildew, and has a sugar content of 11 to 12°Brix, and continuous lightning fastness. A new strawberry variety, NSR090201, with the accession number of KCTC18720P, having these excellent properties, is provided.

또한, 본 발명은 딸기 신품종 NSR090201의 식물체와 다른 딸기 품종을 교배하여 생산된 F1 딸기를 제공한다.In addition, the present invention provides an F 1 strawberry produced by crossing a plant of a new strawberry variety NSR090201 with another strawberry variety.

또한, 본 발명은 아키히메 품종을 모본으로 하고, 수홍 품종을 부본으로 하여 이를 교배시키는 단계를 포함하는, 탄저병, 시들음병 및 흰가루병에 강하고, 당도는 11~12°Brix이며, 연속출뢰성이 우수한 특성을 가지는, 기탁번호가 KCTC18720P인 딸기 신품종 NSR090201의 육종 방법을 제공한다.In addition, the present invention is resistant to anthrax, wilt disease, and powdery mildew, including the step of crossing the Akihime variety as a parent, and the scarlet variety as a counterpart, and has a sugar content of 11 to 12° Brix, and has excellent continuous fastness properties. It provides a method for breeding a new strawberry variety NSR090201 having an accession number of KCTC18720P.

또한, 본 발명은 딸기 신품종 NSR090201를 이용하여 제조된 딸기 가공식품을 제공한다.In addition, the present invention provides a strawberry processed food manufactured using the new strawberry variety NSR090201.

본 발명의 딸기 신품종 NSR090201은 반촉성 적응성으로 탄저병 저항계이며, 시들음병과 흰가루병에도 강한 특성이 있고, 당도가 11.6°Brix로 높으며, 연속출뢰성이 우수한 특징이 있다.The new strawberry variety of the present invention, NSR090201, is an anthrax resistance meter with semi-touch adaptability, has strong properties against wilt and powdery mildew, has a high sugar content of 11.6°Brix, and has excellent continuous lightning fastness.

도 1은 본 발명의 신품종 딸기 NSR090201의 육성 경위를 나타낸 표이다.
도 2는 본 발명의 신품종 딸기 NSR090201의 육성계보도이다.
도 3은 '아키히메' 품종과 '수홍' 품종을 이용한 교배조합의 탄저병균 접종 사진이다. 좌측 계통들은 탄저병균에 저항성을, 우측 계통들은 탄저병균에 감수성을 보이는 것을 알 수 있다.1 is a table showing the development process of the new strawberry NSR090201 of the present invention.
Figure 2 is a genealogical diagram of the new strawberry NSR090201 of the present invention.
Figure 3 is a photograph of anthrax inoculation of a mating combination using the'Akihime' variety and the'Suhong' variety. It can be seen that the left strains are resistant to anthrax, and the right strains are susceptible to anthrax.

본 발명의 목적을 달성하기 위하여, 본 발명은 아키히메 품종을 모본으로 하고, 수홍 품종을 부본으로 하여 이를 교배시켜 얻어진 것으로서, 탄저병, 시들음병 및 흰가루병에 강하고, 당도는 11~12°Brix이며, 연속출뢰성이 우수한 특성을 가지는, 기탁번호가 KCTC18720P인 딸기 신품종 NSR090201 (Fragaria ananassa)을 제공한다.In order to achieve the object of the present invention, the present invention is obtained by crossing the Akihime cultivar as a parent, and the scarlet cultivar as a counterpart, and is resistant to anthrax, wilt disease and powdery mildew, and has a sugar content of 11 to 12° Brix, and continuous We provide a new strawberry variety NSR090201 ( Fragaria ananassa ) with an accession number of KCTC18720P, which has excellent characteristics of fastness.

본 발명의 딸기 신품종 NSR090201은 아래 (1) 내지 (10)의 특성을 가진다:The new strawberry variety NSR090201 of the present invention has the following characteristics (1) to (10):

(1) 과형이 모본 품종인 아키히메는 장원추형인데 비하여 원추형임(1) Akihime, which is the parent cultivar, has a conical shape compared to its long conical shape.

(2) 초형이 모본 품종인 아키히메는 개장형인데 비하여 반개장형임(2) Akihime, which is the parent breed, is a semi-open type compared to the open type.

(3) 개화기는 모본 품종인 아키히메에 비하여 한 달 늦음(3) Flowering period is one month later than the parent variety Akihime

(4) 과색은 연한 진홍색임(4) Super color is light crimson

(5) 과피가 얇음(5) The skin is thin

(6) 과실경도는 245~250g/∮5mm로 중간임(6) Fruit hardness is medium with 245~250g/∮5mm

(7) 당도는 11~12°Brix로 높음(7) High sugar content of 11~12°Brix

(8) 엽병장 및 엽장이 짧음(8) Short leaf length and leaf length

(9) 탄저병, 시들음병, 흰가루병에 강함(9) Resistant to anthrax, wilt, and powdery mildew

(10) 진딧물 발생이 없음.(10) There is no occurrence of aphids.

본 발명의 딸기 신품종 NSR090201은 종자 또는 자묘에 의해 번식하는 품종으로 이해될 수 있다. 상기에서 "자묘"란 줄기가 뻗어가면서 지면과 접촉한 줄기가 발근되어 활착된 것을 말한다. 통상 딸기 재배 농가에서는 자묘에 의한 무성번식을 런너(runner) 번식이라고도 한다.The new strawberry variety NSR090201 of the present invention can be understood as a variety that is propagated by seeds or seedlings. In the above, "jamyo" refers to a stem that has been rooted in contact with the ground as the stem extends. In general, strawberry cultivation farms refer to the asexual breeding by seedlings as runner breeding.

장원추형은 과폭 대비 과장의 비율(과장/과폭을 말함)이 큰 것을 의미하며, 여기서 과장은 길이 방향의 과실의 단면 중 가장 넓은 단면에서 가장 큰 과실의 길이를 의미하며, 과폭은 폭 방향의 과실 단면 중 가장 넓은 단면에서 가장 큰 과실의 길이를 의미한다. 이때 서로 비교 대상이 되는 과실은 동일 화방 동일 위치의 과실을 기준으로 한다. 즉 이는 모든 품종에 대해서 정화방(1화방임) 2번과를 기준으로 하여 산출하든지, 또는 모든 품종에 대해서 정화방 1번과를 기준으로 하여 산출하는 것을 말한다. 통상 정화방 2번과의 비율이 정화방 1번과의 경우보다 기형과의 발생 비율이 적어, 일반적으로 품종의 과실의 특성 대비가 정화방 2번과를 가지고 이루어진다는 점에서 정화방 2번과를 기준으로 하는 것이 바람직하다.Long conical shape means that the ratio of exaggeration to overwidth (referring to exaggeration/overwidth) is large, where exaggeration means the length of the largest fruit in the widest section among the cross-sections in the longitudinal direction, and over-width means the fruit in the width direction. It means the length of the largest fruit in the widest cross section. At this time, the fruits to be compared with each other are based on the fruits in the same location in the same flower room. In other words, this means that for all varieties, it is calculated based on the 2nd department of Jeonghwabang (1hwabangim), or for all varieties based on the 1st department of purificationbang. In general, the ratio of purification room No. 2 is lower than that of purification room No. 1, and the characteristics of the fruit of the variety are generally compared with purification room No. 2. It is desirable to use it as a standard.

본 발명의 일 구현 예에 따른 딸기 신품종 NSR090201은 반촉성(semiforcing) 적응성으로, 통상 반촉성 작형은 4월 중순에서 9월 하순기가 육묘기이고, 9월 하순부터 10월 중순까지가 정식기이며, 11월 하순부터 12월 상순까지가 보온시기, 1월 하순부터 5월 중순이 수확기이다.The new strawberry variety NSR090201 according to an embodiment of the present invention is semiforcing adaptability, and usually semi-touchable cropping is the seedling season from mid-April to late September, and the planting season is from late September to mid-October, and from late November. The warming season is from early December, and the harvest season is from late January to mid-May.

용어 '연속출뢰성'이란, 정화방 후 액화방이 연속해서 나오는 것을 의미하며, 연속출뢰성이 낮은 경우 과수 생산량이 떨어지는 단점이 있다.The term'continuous lightning fastness' means that the liquefied room comes out continuously after the purification room, and when the continuous fastness is low, there is a disadvantage that the production of fruit trees is lowered.

본 명세서에서 '탄저병, 시들음병 및 흰가루병에 강하다'는 표현은 본 발명의 딸기 신품종 NSR090201에 탄저병, 시들음병 및 흰가루병이 발생하지 않는다는 것을 의미한다.In the present specification, the expression "strong against anthrax, wilt and powdery mildew" means that anthrax, wilt and powdery mildew do not occur in the new strawberry variety NSR090201 of the present invention.

우리나라는 2002년 식물신품종보호조약(UPOV, International Union for the Protection of New Variety of Plnats)에 가입하였고, 신품종 보호 대상이 2012년 1월 7일에 딸기를 포함한 전 식물로 확대되어 딸기도 종자산업법에 의한 식물 신품종의 보호 대상이 됨에 따라 로얄티 지불에 따른 농가의 부담의 가중될 것으로 예상된다.Korea joined the International Union for the Protection of New Variety of Plnats (UPOV) in 2002, and the protection of new varieties was expanded to all plants including strawberries on January 7, 2012. It is expected that the burden on farmers due to payment of royalties will increase as new plant varieties are protected by

본 발명은 또한, 기탁번호가 KCTC18720P인 딸기 신품종 NSR090201의 식물체와 다른 딸기 품종을 교배하여 생산된 F1 딸기를 제공한다. 본 발명에 따른 딸기 신품종 NSR090201은 딸기 유묘기에 많이 발생하는 탄저병, 시들음병 및 흰가루병에 대한 병 저항성이 우수하므로, 본 발명의 딸기 신품종 NSR090201은 신품종 육종을 위한 신규의 소재로 이용될 수 있다.The present invention also provides an F 1 strawberry produced by crossing a plant of a new strawberry variety NSR090201 having an accession number of KCTC18720P with another strawberry variety. The new strawberry variety NSR090201 according to the present invention has excellent disease resistance against anthrax, wilt disease, and powdery mildew, which are common in strawberry seedlings, so the new strawberry variety NSR090201 of the present invention can be used as a new material for breeding new varieties.

또한, 본 발명은 아키히메 품종을 모본으로 하고, 수홍 품종을 부본으로 하여 이를 교배시키는 단계를 포함하는, 탄저병, 시들음병 및 흰가루병에 강하고, 당도는 11~12°Brix이며, 연속출뢰성이 우수한 특성을 가지는, 기탁번호가 KCTC18720P인 딸기 신품종 NSR090201의 육종 방법을 제공한다.In addition, the present invention is resistant to anthrax, wilt disease, and powdery mildew, including the step of crossing the Akihime variety as a parent, and the scarlet variety as a counterpart, and has a sugar content of 11 to 12° Brix, and has excellent continuous fastness properties. It provides a method for breeding a new strawberry variety NSR090201 having an accession number of KCTC18720P.

본 발명에 따른 딸기 신품종 NSR090201의 육종 방법은 보다 구체적으로는,The breeding method of the new strawberry variety NSR090201 according to the present invention is more specifically,

(a) 모본 품종인 아키히메 품종을 파종하는 단계;(a) sowing the mother variety Akihime variety;

(b) 부본 품종인 수홍 품종을 파종하는 단계;(b) sowing a cultivar Suhong which is a secondary variety;

(c) 개화 시기에 모본 품종인 아키히메 품종과 부본 품종인 수홍 품종을 인위적으로 교배시키는 단계;(c) artificially crossing the parent cultivar Akihime cultivar and the sub cultivar Suhong cultivar at flowering time;

(d) 교배된 개체의 과실에서 종자를 채종하는 단계;(d) sowing seeds from the fruit of the mated individual;

(e) 종자를 발아·생육시켜 실생 개체를 얻는 단계;(e) germinating and growing seeds to obtain live individuals;

(f) 실생 개체 중 탄저병균(Colletotrichum fructicola)을 접종하여 이병지수(1 이하: 저항성, 1 초과~2 이상: 중도저항성, 2 초과~3 이상: 중도감수성, 3 초과: 감수성)를 기준으로 탄저병균에 저항성을 보이는 계통을 선발하여 자묘에 의하여 증식시키는 단계; 및(f) Anthrax based on the disease index (1 or less: resistance, more than 1-2: moderate resistance, more than 2-3: moderate sensitivity, more than 3: susceptibility) by inoculating anthrax bacteria ( Colletotrichum fructicola ) among live individuals Selecting a lineage showing resistance to bacteria and proliferating it by seedlings; And

(g) 증식된 계통 중 탄저병 저항성을 이병지수(1 이하: 저항성, 1 초과~2 이상: 중도저항성, 2 초과~3 이상: 중도감수성, 3 초과: 감수성)를 기준으로 하여 최종 1계통을 선발하는 단계;를 포함할 수 있다.(g) Among the proliferated strains, the final 1 line was selected based on the morbidity index (less than 1: resistance, more than 1~2: moderate resistance, more than 2~3: moderate sensitivity, more than 3: sensitivity). It may include;

본 발명의 일 구현 예에 따른 딸기 신품종 육종 방법에 있어서, 상기 단계 (a) 및 (b)에서 모본 품종과 부본 품종의 파종은 노지에서 이루어지거나 비닐하우스 등 인위적인 시설에서 이루어질 수 있고, 상기 단계 (a) 및 (b)에서 모본 품종과 부본 품종의 파종 시기는 노지 재배의 경우 우리나라의 기후 조건에서는 5월 중순 경에 이루어질 수 있다. 이때 파종 시기는 모본 품종인 아키히메의 개화 시기와 부본 품종인 수홍의 개화 시기를 맞추기 위하여 같은 시기에 이루어지는 것이 바람직하다.In the method for breeding a new strawberry variety according to an embodiment of the present invention, in the steps (a) and (b), the seeding of the parent variety and the secondary variety may be carried out in the field or in an artificial facility such as a green house, and the step ( In a) and (b), the seeding time of the parent cultivar and the subsidiary cultivar can be carried out around mid-May under the climatic conditions of Korea in the case of outdoor cultivation. At this time, it is preferable that the sowing time is at the same time to match the flowering time of the parent cultivar Akihime and the sub cultivar Suhong.

또한, 상기 단계 (c)에서 인위적 교배 단계는 아래의 실시예에서와 같이 모본의 꽃을 개화 전 봉오리 상태에서 수술을 제거한 후 부본의 꽃가루를 인공수분시켜 이루어지는 것이 바람직하다.In addition, it is preferable that the artificial crossing step in the step (c) is performed by artificial pollination of the pollen of the backbone after removing the stamens from the bud state before flowering of the parent flower as in the following examples.

또한, 상기 단계 (f) 또는 (g)의 계통 선발에서 선발의 기준이 되는 특성은 바람직하게는 이병지수 1 이하의 탄저병 저항성인 것이 바람직하다.In addition, it is preferable that the characteristic that serves as the basis for selection in the system selection of step (f) or (g) is preferably an anthrax resistance having a morbidity index of 1 or less.

본 발명은 또한, 기탁번호가 KCTC18720P인 딸기 신품종 NSR090201를 이용하여 제조된 딸기 가공식품을 제공한다. 상기 딸기 가공식품은 잼, 쥬스를 포함하는 음료, 아이스크림, 유가공품 등일 수 있으나, 이에 제한되지 않는다.In the present invention, the deposit number is KCTC18720P We provide strawberry processed foods manufactured using the new strawberry variety, NSR090201. The strawberry processed foods may be jams, drinks including juice, ice cream, dairy products, etc., but are not limited thereto.

이하, 본 발명을 실시예에 의해 상세히 설명한다. 단, 하기 실시예는 본 발명을 예시하는 것일 뿐, 본 발명의 내용이 하기 실시예에 한정되는 것은 아니다.Hereinafter, the present invention will be described in detail by examples. However, the following examples are only illustrative of the present invention, and the contents of the present invention are not limited to the following examples.

실시예 1. 딸기 신품종 NSR090201의Example 1. Of a new strawberry variety NSR090201 육성 경위Training process

탄저병 저항성 계통 육성을 위해서, 기존 재배 품종 중에서 병충해에는 약하지만 생육이 왕성하고 수량이 많은 아키히메(IT245292) 품종을 모본으로 하고, 탄저병을 비롯한 병해 발생이 거의 없는 수홍(IT232441) 품종을 부본으로 사용하였다(IT번호: 농촌진흥청 국립농업과학원 농업유전자원센터 식물자원 등록번호).For the development of anthrax resistant strains, Akihime (IT245292), which is weak against pests and pests among existing cultivars, has strong growth and has a large quantity, and Suhong (IT232441), which rarely causes diseases such as anthrax, is used as a counterpart. (IT number: plant resource registration number of Agricultural Genetic Resource Center, National Institute of Agricultural Science, Rural Development Administration).

2009년 1~3월에 인공 교배시켰으며, 교배는 모본의 꽃을 개화 전 봉오리 상태에서 핀셋으로 수술을 제거한 후, 부본의 꽃가루를 인공수분시켜 수분 완료한 다음 봉지를 씌운 후 완숙된 과실의 종자를 채취하여 음건한 후 사용하였다. 채종한 종자를 2010년 시판용 혼합상토에서 5월초에 발아시키고, 발아 후 32공 플러그 트레이에 가식한 후 4개월간 생육시켜 2010년 10월 정식하여 실생 우수 개체 90주를 예비 선발하였다.Artificial breeding was carried out in January-March 2009, and the mating was done by removing the stamens with tweezers from the bud before flowering, and then artificially pollinating the pollen of the backbone to complete pollination. Was collected, shaded and used. The harvested seeds were germinated in early May in commercial mixed soil in 2010, and after germination, they were sprouted on a 32 hole plug tray and grown for 4 months. They were planted in October 2010, and 90 strains of excellent seed were preliminarily selected.

2011~2013년에 상기 선발한 실생 개체 90주에 논산 딸기시험장에서 보관 중인 탄저병균(Colletotrichum fructicola) CGF100713 균주를 이용하여 5×105 분생포자/㎖ 농도로 포자현탁액을 만들어 10 ㎖씩 분무접종하였다. 접종 상 완전하게 밀폐하여 과습 상태로 3일 동안 처리 후 재배상으로 옮겨 접종 30일 후에 탄저병 이병지수(1 이하: 저항성, 1 초과~2 이상: 중도저항성, 2 초과~3 이상: 중도감수성, 3 초과: 감수성)를 기준으로 탄저병균에 저항성을 보이는 것을 선발 기준으로 하여 이병지수 1 이하 기준의 14 계통을 선발하였다. 탄저병 저항성 정도는 Denoyes-Rothan B 및 Guerin G(Eur. J. Plant Pathol. (1996) 102:615-621)와 남 등(Plant Pathol J. (2013) 29(3):317-322)의 방법을 변형하여 엽병의 이병지수(1 이하: 저항성, 1 초과~2 이상: 중도저항성, 2 초과~3 이상: 중도감수성, 3 초과: 감수성)를 기준으로 평가하였다. 선발된 14 계통은 자묘에 의하여 증식시켰으며(NSR090201, NSR090202, NSR090203, NSR090204, NSR090205, NSR090206, NSR090207, NSR090208, NSR090209, NSR090210, NSR090211, NSR090212, NSR090213, NSR090214), 증식된 14 계통에 탄저병균(Colletotrichum fructicola)을 접종하고 이병지수를 기준으로 탄저병균에 저항성을 보이는 것을 기준으로 하여 최종 1계통을 선발하였고, 상기 계통을 'NSR090201'로 명명하였다.In 2011-2013, a spore suspension was prepared at a concentration of 5×10 5 conidia/ml using the anthrax (Coletotrichum fructicola ) CGF100713 strain stored in the Nonsan Strawberry Test Center at 90 weeks of the selected seed, and sprayed with 10 ml each. . The inoculation is completely sealed and treated for 3 days in an over-humidified state, and then transferred to a cultivation bed 30 days after inoculation, anthrax morbidity index (1 or less: resistance, more than 1 ~ 2: moderate resistance, more than 2 ~ 3: moderate sensitivity, 3 Exceeding: susceptibility), 14 strains with a morbidity index of 1 or less were selected as a selection criterion that showed resistance to anthrax bacteria. The degree of anthrax resistance was determined by Denoyes-Rothan B and Guerin G (Eur. J. Plant Pathol. (1996) 102:615-621) and Nam et al. (Plant Pathol J. (2013) 29(3):317-322). Was modified and evaluated based on the morbidity index (1 or less: resistance, more than 1-2 or more: moderate resistance, more than 2-3 or more: moderate sensitivity, and more than 3: susceptibility). The selected 14 strains stylized proliferation by jamyo (NSR090201, NSR090202, NSR090203, NSR090204 , NSR090205, NSR090206, NSR090207, NSR090208, NSR090209, NSR090210, NSR090211, NSR090212, NSR090213, NSR090214), anthrax in the proliferation 14 strains (Colletotrichum fructicola ) was inoculated and the final line was selected based on the resistance to anthrax bacteria based on the disease index, and the line was named'NSR090201'.

2014년부터 2015년까지 2년 동안 생산력검정시험을 진행하여, 상기 1계통을 선발 확정하여 'NSR090201'으로 품종명을 부여하였고, 상기 딸기 신품종을 한국생명공학연구원 생물자원센터(KCTC)에 2018년 10월 18일자로 '탄저병 저항성 딸기 신품종 NSR090201 (Fragaria ananassa)' 명칭으로 기탁하였다(기탁번호 : KCTC18720P). 도 1은 본 발명의 딸기 신품종 NSR090201의 육성 경위를 보여준다.The productivity test was conducted for two years from 2014 to 2015, and the first strain was selected and given a variety name as'NSR090201', and the new strawberry variety was sent to the Korea Research Institute of Bioscience and Biotechnology Biological Resource Center (KCTC) in 2018. As of May 18, it was deposited under the name of'Anthrax-Resistant Strawberry New Variety NSR090201 ( Fragaria ananassa )' (Accession No.: KCTC18720P). 1 shows the development process of the new strawberry variety NSR090201 of the present invention.

실시예 2. 딸기 신품종 NSR090201의 특성Example 2. Characteristics of the new strawberry variety NSR090201

NSR090201의 품종 특성 조사는 대조품종을 아키히메로 하였으며, 시험성적 평가의 기준은 농림수산식품부 국립종자원에서 2008년에 발표한 <신품종 심사를 위한 작물별 특헝조사요령(딸기)>에 따라 측정하였고, 실측치는 10개의 개체를 임의로 취하여 분석하였다.The cultivar characteristics survey of NSR090201 was conducted using the control variety as Akihime, and the criteria for test score evaluation were measured according to the <Special Survey Guidelines for New Varieties (Strawberries)> published in 2008 by the Ministry of Food, Agriculture, Forestry and Fisheries. , The measured value was analyzed by randomly taking 10 individuals.

Figure 112018123855726-pat00001
Figure 112018123855726-pat00001

특성 분석 결과, 딸기 신품종 NSR090201는 과형이 모본 품종인 아키히메는 장원추형인데 비하여 원추형이고, 초형이 모본 품종인 아키히메는 개장형인데 비하여 반개장형이며, 1화방 기준 개화기는 모본 품종인 아키히메에 비하여 한 달 정도 늦은 것으로 분석되었다. 또한, 모본 품종인 아키히메는 휴면성이 얕으나, 신품종은 휴면성이 깊은 것으로 확인되었다. 또한, 과색은 연한 진홍색이고, 과피가 얇으며, 과실경도는 248g/∮5mm로 중간 수준이고, 과실의 당도는 11.6°Brix로 높아 맛이 우수한 것으로 확인되었다. 또한, 엽병장 및 엽장이 짧고, 탄저병 외에 시들음병, 흰가루병이 거의 발생하지 않는 것으로 확인되었으며, 잿빛곰팡이병은 약간 발생하는 것으로 확인되었다. 또한, 진딧물 발생은 없고 응애는 약하게 발생하는 것으로 확인되었다.As a result of the characteristic analysis, the new strawberry variety NSR090201 has a conical shape compared to the long conical type of the parent cultivar, Akihime, which is the parent cultivar, and the Akihime, which is the parent cultivar, has a semi-open type compared to the open type. It was analyzed that it was about a month later than that. In addition, it was confirmed that the parent variety Akihime has shallow dormancy, but the new variety has deep dormancy. In addition, the fruit color was light crimson, the skin was thin, the fruit hardness was 248g/∮5mm, which was a medium level, and the sweetness of the fruit was 11.6°Brix, so the taste was excellent. In addition, it was confirmed that the petiole and leaf length were short, and wilting disease and powdery mildew were hardly occurred in addition to anthrax, and gray mold disease was found to occur slightly. In addition, it was confirmed that there was no occurrence of aphids and the mites were weakly generated.

딸기 신품종 NSR090201는 충남 및 전국일원 반촉성 재배지역에 적응가능할 것으로 판단되며, 세력이 약해 지속적인 비배관리가 요구되고, 초세에 비하여 꽃수가 많으므로 꽃솎음이 필요한 것으로 사료되었다. 또한, 액아발생이 많은 편으로, 생육 초기 발생하는 액아를 제거해야하는 유의점이 있다.The new strawberry variety, NSR090201, is considered to be adaptable to semi-contact cultivation areas in Chungnam and nationwide, and its weak power requires continuous cultivation management, and it is considered that flower thinning is necessary because the number of flowers is larger than that of the superseed. In addition, there is a point of caution in that axillary sprouts that occur in the early stages of growth must be removed because axillary is often generated.

한국생명공학연구원Korea Research Institute of Bioscience and Biotechnology KCTC18720PKCTC18720P 2018101820181018

Claims (5)

아키히메 품종을 모본으로 하고, 수홍 품종을 부본으로 하여 이를 교배시켜 얻어진 것으로서, 탄저병, 시들음병 및 흰가루병에 강하고, 당도는 11~12°Brix이며, 연속출뢰성이 우수한 특성을 가지는, 기탁번호가 KCTC18720P인 딸기 신품종 NSR090201 (Fragaria ananassa).It is obtained by crossing the Akihime cultivar as a parent, and the scarlet cultivar as a counterpart, and is resistant to anthrax, wilt disease and powdery mildew, has a sugar content of 11 to 12°Brix, and has excellent characteristics of continuous lightning fastness. Phosphorus strawberry new variety NSR090201 ( Fragaria ananassa ). 제1항의 딸기 신품종 NSR090201의 식물체와 다른 딸기 품종을 교배하여 생산된 F1 딸기.The F 1 strawberry produced by crossing the plant of the new strawberry variety NSR090201 of claim 1 with other strawberry varieties. 아키히메 품종을 모본으로 하고, 수홍 품종을 부본으로 하여 이를 교배시키는 단계를 포함하는, 탄저병, 시들음병 및 흰가루병에 강하고, 당도는 11~12°Brix이며, 연속출뢰성이 우수한 특성을 가지는, 기탁번호가 KCTC18720P인 딸기 신품종 NSR090201 (Fragaria ananassa)의 육종 방법.The deposit number is strong against anthrax, wilt disease and powdery mildew, including the step of crossing the Akihime variety as a parent, and the scarlet variety as a counterpart, and has a sugar content of 11-12°Brix, and has excellent characteristics of continuous lightning fastness. Breeding method of the new strawberry variety NSR090201 ( Fragaria ananassa ) with KCTC18720P. 제3항에 있어서,
(a) 모본 품종인 아키히메 품종을 파종하는 단계;
(b) 부본 품종인 수홍 품종을 파종하는 단계;
(c) 개화 시기에 모본 품종인 아키히메 품종과 부본 품종인 수홍 품종을 인위적으로 교배시키는 단계;
(d) 교배된 개체의 과실에서 종자를 채종하는 단계;
(e) 종자를 발아·생육시켜 실생 개체를 얻는 단계;
(f) 실생 개체 중 탄저병균(Colletotrichum fructicola)을 접종하여 이병지수(1 이하: 저항성, 1 초과~2 이상: 중도저항성, 2 초과~3 이상: 중도감수성, 3 초과: 감수성)를 기준으로 탄저병균에 저항성을 보이는 계통을 선발하여 자묘에 의하여 증식시키는 단계; 및
(g) 증식된 계통 중 탄저병 저항성을 이병지수(1 이하: 저항성, 1 초과~2 이상: 중도저항성, 2 초과~3 이상: 중도감수성, 3 초과: 감수성)를 기준으로 하여 최종 1계통을 선발하는 단계;를 포함하는 것을 특징으로 하는, 기탁번호가 KCTC18720P인 딸기 신품종 NSR090201 (Fragaria ananassa)의 육종 방법.The method of claim 3,
(a) sowing the mother variety Akihime variety;
(b) sowing a cultivar Suhong which is a secondary variety;
(c) artificially crossing the parent cultivar Akihime cultivar and the sub cultivar Suhong cultivar at flowering time;
(d) sowing seeds from the fruit of the mated individual;
(e) germinating and growing seeds to obtain live individuals;
(f) Anthrax based on the disease index (1 or less: resistance, more than 1-2: moderate resistance, more than 2-3: moderate sensitivity, more than 3: susceptibility) by inoculating anthrax bacteria ( Colletotrichum fructicola ) among live individuals Selecting a lineage showing resistance to bacteria and proliferating it by seedlings; And
(g) Among the proliferated strains, the final 1 line was selected based on the morbidity index (less than 1: resistance, more than 1~2: moderate resistance, more than 2~3: moderate sensitivity, more than 3: sensitivity). The method of breeding a new strawberry variety NSR090201 ( Fragaria ananassa ), characterized in that it comprises, the accession number of KCTC18720P. 제1항의 딸기 신품종 NSR090201 (Fragaria ananassa)를 이용하여 제조된 딸기 가공식품.Strawberry processed foods manufactured using the new strawberry variety of claim 1 NSR090201 ( Fragaria ananassa ).
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