KR102130242B1 - Processed ballast water test method for treated ship using dyeing mixing reagent - Google Patents
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- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 128
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 119
- 238000004043 dyeing Methods 0.000 title abstract description 32
- 238000002156 mixing Methods 0.000 title abstract description 12
- 238000010998 test method Methods 0.000 title description 6
- PGSADBUBUOPOJS-UHFFFAOYSA-N neutral red Chemical compound Cl.C1=C(C)C(N)=CC2=NC3=CC(N(C)C)=CC=C3N=C21 PGSADBUBUOPOJS-UHFFFAOYSA-N 0.000 claims abstract description 44
- 238000007689 inspection Methods 0.000 claims abstract description 31
- 238000000034 method Methods 0.000 claims abstract description 27
- 239000012153 distilled water Substances 0.000 claims abstract description 10
- 238000002360 preparation method Methods 0.000 claims description 26
- 238000006243 chemical reaction Methods 0.000 claims description 9
- 238000003756 stirring Methods 0.000 claims description 9
- 238000013019 agitation Methods 0.000 claims description 8
- 238000010186 staining Methods 0.000 claims description 7
- 239000011521 glass Substances 0.000 claims description 5
- 230000009257 reactivity Effects 0.000 claims description 5
- 239000012530 fluid Substances 0.000 claims description 4
- 238000002347 injection Methods 0.000 claims description 4
- 239000007924 injection Substances 0.000 claims description 4
- COXVTLYNGOIATD-HVMBLDELSA-N CC1=C(C=CC(=C1)C1=CC(C)=C(C=C1)\N=N\C1=C(O)C2=C(N)C(=CC(=C2C=C1)S(O)(=O)=O)S(O)(=O)=O)\N=N\C1=CC=C2C(=CC(=C(N)C2=C1O)S(O)(=O)=O)S(O)(=O)=O Chemical compound CC1=C(C=CC(=C1)C1=CC(C)=C(C=C1)\N=N\C1=C(O)C2=C(N)C(=CC(=C2C=C1)S(O)(=O)=O)S(O)(=O)=O)\N=N\C1=CC=C2C(=CC(=C(N)C2=C1O)S(O)(=O)=O)S(O)(=O)=O COXVTLYNGOIATD-HVMBLDELSA-N 0.000 abstract description 20
- 229960003699 evans blue Drugs 0.000 abstract description 20
- 239000013535 sea water Substances 0.000 abstract description 12
- XOSXWYQMOYSSKB-LDKJGXKFSA-L water blue Chemical compound CC1=CC(/C(\C(C=C2)=CC=C2NC(C=C2)=CC=C2S([O-])(=O)=O)=C(\C=C2)/C=C/C\2=N\C(C=C2)=CC=C2S([O-])(=O)=O)=CC(S(O)(=O)=O)=C1N.[Na+].[Na+] XOSXWYQMOYSSKB-LDKJGXKFSA-L 0.000 abstract description 8
- 239000000975 dye Substances 0.000 description 15
- 238000012360 testing method Methods 0.000 description 6
- 230000000694 effects Effects 0.000 description 4
- 230000007935 neutral effect Effects 0.000 description 3
- PAYRUJLWNCNPSJ-UHFFFAOYSA-N Aniline Chemical compound NC1=CC=CC=C1 PAYRUJLWNCNPSJ-UHFFFAOYSA-N 0.000 description 2
- 210000004102 animal cell Anatomy 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 239000003086 colorant Substances 0.000 description 2
- 239000010419 fine particle Substances 0.000 description 2
- 210000003712 lysosome Anatomy 0.000 description 2
- 230000001868 lysosomic effect Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000006116 polymerization reaction Methods 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- CBENFWSGALASAD-UHFFFAOYSA-N Ozone Chemical compound [O-][O+]=O CBENFWSGALASAD-UHFFFAOYSA-N 0.000 description 1
- 125000002091 cationic group Chemical group 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 238000005868 electrolysis reaction Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
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- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/4833—Physical analysis of biological material of solid biological material, e.g. tissue samples, cell cultures
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Abstract
Description
본 발명은 염색 혼합시약을 이용한 처리된 선박 평형수 검사방법에 관한 것이다. 보다 상세하게는 육상과 같이 선상에서도 선박평형수 처리장치를 통해 처리된 처리수 성분 검사를 간편하게 수행할 수 있고, 그 결과를 확인할 수 있고, 뉴트럴 레드(Neutral Red), 에반스 블루(Evan's Blue), 아닐린 블루(Aniline Blue), 증류수의 특정 비율 혼합 시약으로 해수의 염색에서 시약이 서로 뭉쳐 염색되는 것을 최대한 방지하여 검사 결과의 정확도를 높이는 염색 혼합시약을 이용한 처리된 선박 평형수 검사방법에 관한 것이다.The present invention relates to a method for inspecting ballast water in a treated vessel using a dye mixed reagent. In more detail, it is possible to easily inspect the treated water component through the ballast water treatment device on the ship, such as on the ground, to check the results, Neutral Red, Evan's Blue, Aniline Blue (Aniline Blue) relates to a method for inspecting a ballast water in a treated vessel using a dyeing mixed reagent, which increases the accuracy of the test result by preventing the reagents from being lumped together and stained with dyes of seawater with a specific ratio mixing reagent of distilled water.
선박 평형수에 포함된 외래종과 침전물의 배출에 의해서 해양이 오염되고 생태계가 파괴되는 현상에 대한 논란이 제기되어왔다. Controversy has been raised about the phenomenon of marine pollution and ecosystem destruction by the discharge of foreign species and sediments contained in ballast water.
1988년 캐나다와 호주가 자국 해역에 유해한 종의 출현 문제를 경험하고, IMO의 해양환경보호위원회에 이에 대한 문제를 제기함에 따라, 2004년 2월 홍콩 외교회의(International Conference)에서 선박평형수관리협약(2004)이 채택되었다. 이 협약은 세계 상선 선복량의 35% 이상을 보유한 30개 회원국이 협약을 비준한 날로부터 12개월 후 발효될 예정이었으며, 2016년 9월 8일에 핀란드가 비준서를 기탁하면서 발효요건이 충족돼 2017년 9월 8일부터 세계적으로 발효되었다.In 1988, Canada and Australia experienced the problem of the emergence of species harmful to their waters and raised the issue with the IMO's Marine Environment Protection Committee. (2004) was adopted. The agreement was to take effect 12 months after the date of ratification of the agreement by 30 member countries with more than 35% of the world's merchant ships, and on September 8, 2016, Finland deposited the instruments of ratification and the fermentation requirements were met. It came into effect globally from September 8.
협약의 발효일자 이후에 건조된 협약적용 대상 선박들은 성능기준을 만족하기 위한 BWTS(Ballast Water Treatment System)의 탑재가 요구되며, 협약의 발효일자 전에 건조된 선박들은 이전 IOPP 정기검사의 완료날짜에 따라 협약발효일 후의 첫 번째 또는 두 번째 정기검사 시까지 해당기준의 만족이 요구된다. Ships subject to the agreement that were built after the effective date of the Convention are required to be equipped with a Ballast Water Treatment System (BWTS) to meet performance standards, and ships built before the effective date of the Convention are subject to the completion date of the previous IOPP regular inspection. Satisfaction is required until the first or second regular inspection after the effective date of the agreement.
따라서, 육상에서는 선박평형수 내에 존재하는 BWTS의 IMO나 USCG 형식 승인을 위한 연구가 활발히 진행되고 있다.Therefore, studies on BWTS IMO or USCG type approval existing in ballast water are actively being conducted on land.
하지만, PSC, USCG의 수검에 대비하여 선상에서 이루어지는 선박 평형수 처리장치의 처리수 성분 검사 시스템은 없는 실정이고, 육상과 마찬가지로 선상에서도 선박평형수 처리장치를 통해 나온 처리수의 성분을 검사할 수 있는 환경을 구축하는 것이 필요한 실정이다.However, in preparation for PSC and USCG inspections, there is no system for inspecting the treated water component of the ballast water treatment device on board, and as on land, it is possible to inspect the components of the treated water from the ballast water treatment device on board. It is necessary to establish an environment in which there is.
선행기술문헌 : 대한민국 등록특허공보 제1551816호(2015.9.9 공고)Prior Art Document: Republic of Korea Registered Patent Publication No. 1551816 (Announcement of 2015.9.9)
본 발명은 상기와 같은 문제점을 해결하기 위해 안출된 것으로, 선상에서도 선박평형수 처리장치를 통해 처리된 처리수 성분을 간이검사할 수 있는 염색 혼합시약을 이용한 처리된 선박 평형수 검사방법을 제공하는 데 그 목적이 있다.The present invention has been devised to solve the above problems, and provides a method for inspecting the ballast water in a treated vessel using a dyeing mixed reagent capable of performing a simple inspection of the treated water component through the ballast water treatment device even on board. It has its purpose.
상기한 목적을 달성하기 위하여 본 발명에 따르는 염색 혼합시약을 이용한 처리된 선박 평형수 검사방법은 선박 평형수 처리수를 준비하고 반응 용기에 담는 준비 단계; 준비 단계에서 준비된 선박 평형수 처리수를 염색시킬 시약을 제조하는 시약 제조 단계; 선박 평형수 처리수가 담긴 반응 용기에 시약 제조 단계에서 제조된 시약을 투입하는 시약 투입 단계; 시약 투입 단계에서 시약이 투입된 선박 평형수 처리수를 일정 시간 교반을 수행하는 교반 단계; 시약이 투입되어 반응된 선박 평형수 처리수를 확대경으로 죽은 식물성 플랑크톤과 동물성 플랑크톤의 분포를 검사하는 제1 검사 단계; 및 광원공급을 통해 비전 카메라 검사를 수행하여 살아있는 식물성 플랑크톤을 분포를 검사하는 제2 검사 단계를 포함할 수 있다.In order to achieve the above object, a method for inspecting treated ballast water using a dyeing and mixing reagent according to the present invention includes a preparation step of preparing a ballast water treated water and placing it in a reaction vessel; A reagent preparation step of preparing a reagent for staining the treated ballast water prepared in the preparation step; A reagent input step of inputting the reagent prepared in the reagent preparation step into the reaction vessel containing the ballast water treatment vessel; A stirring step of performing agitation of the ballast water treated with the reagent for a predetermined time in the reagent input step; A first inspection step of inspecting the distribution of phytoplankton and animal plankton dead by using a magnifying glass in the vessel ballast water treated with reagents; And a second inspection step of inspecting the distribution of live phytoplankton by performing a vision camera inspection through supply of a light source.
또한, 선박 평형수 처리수를 준비하고 반응 용기에 담는 준비 단계; 준비 단계에서 준비된 선박 평형수 처리수를 염색시킬 시약을 제조하는 시약 제조 단계; 선박 평형수 처리수가 담긴 반응 용기에 시약 제조 단계에서 제조된 시약을 투입하는 시약 투입 단계; 시약 투입 단계에서 시약이 투입된 선박 평형수 처리수를 일정 시간 교반을 수행하는 교반 단계; 시약이 투입되어 반응된 선박 평형수 처리수를 확대경으로 죽은 식물성 플랑크톤과 동물성 플랑크톤의 분포를 검사하는 제1 검사 단계; 및 광원공급을 통해 비전 카메라 검사를 수행하여 살아있는 식물성 플랑크톤을 분포를 검사하는 제2 검사 단계를 포함하고, 교반 단계는 시약 투입 단계에서 투입된 시약과 반응할 선박 평형수 처리수의 반응도를 높이기 위해 유체에 열 에너지를 공급하는 것을 포함할 수 있다.In addition, a preparation step of preparing the ballast water treated water and placing it in a reaction vessel; A reagent preparation step of preparing a reagent for staining the treated ballast water prepared in the preparation step; A reagent input step of inputting the reagent prepared in the reagent preparation step into the reaction vessel containing the ballast water treatment vessel; A stirring step of performing agitation of the ballast water treated with the reagent for a predetermined time in the reagent input step; A first inspection step of inspecting the distribution of phytoplankton and animal plankton dead by using a magnifying glass in the vessel ballast water treated with reagents; And a second inspection step of inspecting the distribution of live phytoplankton by performing a vision camera inspection through supply of a light source, and the agitation step is performed to increase the reactivity of the ballast water treated water that will react with the reagent added in the reagent introduction step. It may include supplying thermal energy to.
또한, 시약 투입 단계의 투입되는 시약은 뉴트럴 레드(Neutral Red) 1.65g, 에반스 블루(Evan's Blue) 0.5g, 증류수 1000mL 비율로 혼합된 것을 포함할 수 있다.In addition, the reagent to be added in the reagent injection step may include a mixture of Neutral Red 1.65 g, Evan's Blue 0.5 g, and distilled water at a rate of 1000 mL.
또한, 시약 투입 단계의 투입되는 시약은 뉴트럴 레드(Neutral Red) 1.65g, 아닐린 블루(Aniline Blue) 10g, 증류수 1000mL 비율로 혼합된 것을 포함할 수 있다.In addition, the reagent to be added in the reagent input step may include a mixture of Neutral Red 1.65 g, Aniline Blue 10 g, and distilled water at a rate of 1000 mL.
본 발명에 의하면 육상과 같이 선상에서도 선박평형수 처리장치를 통해 처리된 처리수 성분 검사를 간편하게 수행할 수 있고, 그 결과를 확인할 수 있는 데 그 효과가 있다.According to the present invention, it is possible to conveniently perform the treatment of the treated water component through the ballast water treatment device on the ship, such as on land, and it is effective in confirming the result.
또한, 본 발명에 의하면, 뉴트럴 레드(Neutral Red), 에반스 블루(Evan's Blue), 아닐린 블루(Aniline Blue), 증류수의 특정 비율 혼합 시약으로 해수의 염색에서 시약이 서로 뭉쳐 염색되는 것을 최대한 방지하여 검사 결과의 정확도를 높이는 데 그 효과가 있다.In addition, according to the present invention, Neutral Red (Neutral Red), Evans Blue (Evan's Blue), aniline blue (Aniline Blue), mixing reagents in a specific ratio of distilled water to prevent the reagents from being stained together by dyeing in seawater staining It is effective in improving the accuracy of the results.
도 1은 본 발명의 바람직한 실시예에 따른 염색 혼합시약을 이용한 처리된 선박 평형수 검사방법의 순서도,
도 2는 본 발명의 바람직한 실시예에 따른 염색 혼합시약을 이용한 처리된 선박 평형수 검사방법의 선박 평형수를 뉴트럴 레드(Neutral Red)로 단독 염색한 것을 나타낸 사진,
도 3은 본 발명의 바람직한 실시예에 따른 염색 혼합시약을 이용한 처리된 선박 평형수 검사방법의 선박 평형수를 에반스 블루(Evan's Blue)로 단독 염색한 것을 나타낸 사진,
도 4는 본 발명의 바람직한 실시예에 따른 염색 혼합시약을 이용한 처리된 선박 평형수 검사방법의 선박 평형수를 뉴트럴 레드(Neutral Red)와 에반스 블루(Evan's Blue)가 포함된 혼합 시약으로 염색한 것을 나타낸 사진,
도 5는 본 발명의 바람직한 실시예에 따른 염색 혼합시약을 이용한 처리된 선박 평형수 검사방법의 20분 처리한 선박 평형수 처리수를 뉴트럴 레드(Neutral Red)와 에반스 블루(Evan's Blue)가 포함된 혼합 시약으로 염색한 것을 나타낸 사진,
도 6은 본 발명의 바람직한 실시예에 따른 염색 혼합시약을 이용한 처리된 선박 평형수 검사방법의 80분 처리한 선박 평형수 처리수를 뉴트럴 레드(Neutral Red)와 에반스 블루(Evan's Blue)가 포함된 혼합 시약으로 염색한 것을 나타낸 사진,
도 7은 본 발명의 바람직한 실시예에 따른 염색 혼합시약을 이용한 처리된 선박 평형수 검사방법의 선박 평형수를 뉴트럴 레드(Neutral Red)와 아닐린 블루(Aniline Blue)가 포함된 혼합 시약으로 염색한 것을 나타낸 사진,
도 8은 본 발명의 바람직한 실시예에 따른 염색 혼합시약을 이용한 처리된 선박 평형수 검사방법의 20분 처리한 선박 평형수 처리수를 뉴트럴 레드(Neutral Red)와 아닐린 블루(Aniline Blue)가 포함된 혼합 시약으로 염색한 것을 나타낸 사진, 및
도 9는 본 발명의 바람직한 실시예에 따른 염색 혼합시약을 이용한 처리된 선박 평형수 검사방법의 80분 처리한 선박 평형수 처리수를 뉴트럴 레드(Neutral Red)와 아닐린 블루(Aniline Blue)가 포함된 혼합 시약으로 염색한 것을 나타낸 사진이다.1 is a flow chart of a method for inspecting ballast water in a treated vessel using a dye mixing reagent according to a preferred embodiment of the present invention,
FIG. 2 is a photograph showing that the ballast water of the treated ballast water inspection method using a dyeing mixed reagent according to a preferred embodiment of the present invention is stained with Neutral Red alone.
Figure 3 is a photograph showing that the ballast water of the treated ballast water inspection method using a dyeing mixed reagent according to a preferred embodiment of the present invention is stained with Evan's Blue alone,
Figure 4 is a stained with a mixed reagent containing the neutral red (Neutral Red) and Evans Blue (Evan's Blue) of the ballast water of the method for testing the treated ballast water using a dye mixed reagent according to a preferred embodiment of the present invention Photography,
FIG. 5 includes neutral red and evan's blue of ballast water treated with 20 minutes of the ballast water treatment method of the treated ballast water using a dye mixed reagent according to a preferred embodiment of the present invention. Pictures showing dyeing with mixed reagents,
FIG. 6 shows neutral red and Evan's blue of ballast water treated with 80 minutes of ballast water treatment method of the ballast water treatment method using a dyeing mixed reagent according to a preferred embodiment of the present invention. Pictures showing dyeing with mixed reagents,
7 is that the ballast water of the vessel ballast water treatment method using the dyeing mixed reagent according to a preferred embodiment of the present invention is stained with a mixed reagent containing Neutral Red (Neutral Red) and aniline blue (Aniline Blue) Photography,
Figure 8 is a 20-minute treatment of the ballast water treatment method of the ballast water treatment method using a dyeing mixed reagent according to a preferred embodiment of the present invention contains neutral red (Neutral Red) and aniline blue (Aniline Blue) Pictures showing staining with mixed reagents, and
FIG. 9 shows neutral red and aniline blue of treated ballast water treated with 80 minutes of the ballast water treatment method of the treated vessel using a dyeing mixed reagent according to a preferred embodiment of the present invention. It is a picture showing the dyeing with a mixed reagent.
이하, 본 발명의 바람직한 실시예를 첨부된 도면들을 참조하여 상세히 설명한다. 우선 각 도면의 구성 요소들에 참조 부호를 부가함에 있어서, 동일한 구성 요소들에 대해서는 비록 다른 도면상에 표시되더라도 가능한 한 동일한 부호를 가지도록 하고 있음에 유의해야 한다. 또한, 본 발명을 설명함에 있어, 관련된 공지 구성 또는 기능에 대한 구체적인 설명이 본 발명의 요지를 흐릴 수 있다고 판단되는 경우에는 그 상세한 설명은 생략한다. 또한, 이하에서 본 발명의 바람직한 실시예를 설명할 것이나, 본 발명의 기술적 사상은 이에 한정하거나 제한되지 않고 당업자에 의해 변형되어 다양하게 실시될 수 있음은 물론이다.Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. First, when adding reference numerals to the components of each drawing, it should be noted that the same components have the same reference numerals as possible even though they are displayed on different drawings. In addition, in describing the present invention, when it is determined that detailed descriptions of related well-known structures or functions may obscure the subject matter of the present invention, detailed descriptions thereof will be omitted. In addition, although preferred embodiments of the present invention will be described below, the technical spirit of the present invention is not limited to or limited thereto, and can be variously implemented by a person skilled in the art.
본 발명은 염색 혼합시약을 이용한 처리된 선박 평형수 검사방법(S100)에 관한 것이다. 보다 상세하게는 육상과 같이 선상에서도 선박평형수 처리장치를 통해 처리된 처리수 성분 검사를 간편하게 수행할 수 있고, 그 결과를 확인할 수 있고, 뉴트럴 레드(Neutral Red), 에반스 블루(Evan's Blue), 아닐린 블루(Aniline Blue), 증류수의 특정 비율 혼합 시약으로 해수의 염색에서 시약이 서로 뭉쳐 염색되는 것을 최대한 방지하여 검사 결과의 정확도를 높이는 염색 혼합시약을 이용한 처리된 선박 평형수 검사방법(S100)에 관한 것이다.The present invention relates to a method for inspecting ballast water in a treated vessel using a dye mixed reagent (S100). In more detail, it is possible to easily inspect the treated water component through the ballast water treatment device on the ship, such as on the ground, to check the results, Neutral Red, Evan's Blue, Aniline Blue, a specific ratio of distilled water, is a mixed ratio of reagents.It is used to check the ballast water in a treated vessel using a dyeing and mixing reagent that improves the accuracy of the test results by preventing the reagents from sticking together and dying in the dyeing of sea water (S100). It is about.
도 1은 본 발명의 바람직한 실시예에 따른 염색 혼합시약을 이용한 처리된 선박 평형수 검사방법(S100)의 순서도이고, 도 2는 본 발명의 바람직한 실시예에 따른 염색 혼합시약을 이용한 처리된 선박 평형수 검사방법(S100)의 선박 평형수를 뉴트럴 레드(Neutral Red)로 단독 염색한 것을 나타낸 사진이며, 도 3은 본 발명의 바람직한 실시예에 따른 염색 혼합시약을 이용한 처리된 선박 평형수 검사방법(S100)의 선박 평형수를 에반스 블루(Evan's Blue)로 단독 염색한 것을 나타낸 사진이고, 도 4는 본 발명의 바람직한 실시예에 따른 염색 혼합시약을 이용한 처리된 선박 평형수 검사방법(S100)의 선박 평형수를 뉴트럴 레드(Neutral Red)와 에반스 블루(Evan's Blue)가 포함된 혼합 시약으로 염색한 것을 나타낸 사진이며, 도 5는 본 발명의 바람직한 실시예에 따른 염색 혼합시약을 이용한 처리된 선박 평형수 검사방법(S100)의 20분 처리한 선박 평형수 처리수를 뉴트럴 레드(Neutral Red)와 에반스 블루(Evan's Blue)가 포함된 혼합 시약으로 염색한 것을 나타낸 사진이고, 도 6은 본 발명의 바람직한 실시예에 따른 염색 혼합시약을 이용한 처리된 선박 평형수 검사방법(S100)의 80분 처리한 선박 평형수 처리수를 뉴트럴 레드(Neutral Red)와 에반스 블루(Evan's Blue)가 포함된 혼합 시약으로 염색한 것을 나타낸 사진이며, 도 7은 본 발명의 바람직한 실시예에 따른 염색 혼합시약을 이용한 처리된 선박 평형수 검사방법(S100)의 선박 평형수를 뉴트럴 레드(Neutral Red)와 아닐린 블루(Aniline Blue)가 포함된 혼합 시약으로 염색한 것을 나타낸 사진이고, 도 8은 본 발명의 바람직한 실시예에 따른 염색 혼합시약을 이용한 처리된 선박 평형수 검사방법(S100)의 20분 처리한 선박 평형수 처리수를 뉴트럴 레드(Neutral Red)와 아닐린 블루(Aniline Blue)가 포함된 혼합 시약으로 염색한 것을 나타낸 사진이며, 도 9는 본 발명의 바람직한 실시예에 따른 염색 혼합시약을 이용한 처리된 선박 평형수 검사방법(S100)의 80분 처리한 선박 평형수 처리수를 뉴트럴 레드(Neutral Red)와 아닐린 블루(Aniline Blue)가 포함된 혼합 시약으로 염색한 것을 나타낸 사진이다.1 is a flow chart of a method for inspecting a ballast water of a treated vessel using a dyeing mixing reagent according to a preferred embodiment of the present invention (S100), and FIG. 2 is a ballasting of a treated vessel using a dyeing mixing reagent according to a preferred embodiment of the present invention A photograph showing that the ballast water of the water test method (S100) is dyed with Neutral Red alone, and FIG. 3 is a method for testing the ballast water of a treated vessel using a dyeing mixed reagent according to a preferred embodiment of the present invention ( S100) is a photograph showing the ship's ballast water alone stained with Evan's Blue, and FIG. 4 is a vessel of the ballast water inspection method (S100) treated with a dye mixed reagent according to a preferred embodiment of the present invention A photograph showing that the ballast water is dyed with a mixed reagent containing Neutral Red and Evan's Blue, and FIG. 5 is a ballast water treated with a dye mixed reagent according to a preferred embodiment of the present invention. This is a photograph showing that the ballast water treated with 20 minutes of the inspection method (S100) is stained with a mixed reagent containing Neutral Red and Evan's Blue, and FIG. 6 is a preferred embodiment of the present invention. Dyeing of the ballast water treated with 80 minutes of the ballast water test method (S100) using the dyeing mixed reagent according to the example was stained with a mixed reagent containing Neutral Red and Evan's Blue. 7 is a neutral ball (Neutral Red) and aniline blue (Aniline Blue) of the ballast water of the vessel ballast water test method (S100) using a dyeing mixed reagent according to a preferred embodiment of the present invention Figure 8 is a photograph showing the dyeing with the mixed reagent included, Figure 8 is a ballast water treated with 20 minutes of the ballast water treatment method (S100) treated with a dye mixed reagent according to a preferred embodiment of the present invention neutral It is a mixed reagent containing Neutral Red and Aniline Blue. It is a picture showing the dyed, and FIG. 9 shows the neutral ball (Neutral Red) of the ballast water treated by 80 minutes of the ballast water test method (S100) treated using the dyeing mixed reagent according to a preferred embodiment of the present invention. And Aniline Blue.
도 1을 참조하면, 염색 혼합시약을 이용한 처리된 선박 평형수 검사방법(S100)은 준비 단계(S10), 시약 제조 단계(S20), 시약 투입 단계(S30), 교반 단계(S40), 제1 검사 단계(S50), 및 제2 검사 단계(S60)를 포함하여 구성된다.Referring to FIG. 1, the method for inspecting the ballast water of a treated vessel using a dye mixed reagent (S100) is a preparation step (S10), a reagent preparation step (S20), a reagent input step (S30), a stirring step (S40), and a first It comprises a test step (S50), and a second test step (S60).
이하, 준비 단계(S10)부터 상세히 설명하기로 한다.Hereinafter, it will be described in detail from the preparation step (S10).
준비 단계(S10)는 선박 평형수 처리수를 준비하고 반응 용기에 담는 단계이다.The preparation step (S10) is a step of preparing the ballast water treatment vessel and placing it in a reaction vessel.
선박 평형수 처리수는 선박 평형수를 전기분해 방식, 오존 방식, 및 자외선방식를 포함하는 처리를 통해 선박 평형수에 포함된 유해 수상생물과 병원균을 제거, 무해화한 것을 말한다.The ballast water treatment vessel refers to the removal and harmless of harmful aquatic organisms and pathogens contained in the ballast water by treating the ballast water by electrolysis, ozone, and ultraviolet treatment.
시약 제조 단계(S20)는 준비 단계(S10)에서 준비된 선박 평형수 처리수를 염색시킬 시약을 제조하는 단계로, 사용되는 시약의 종류는 뉴트럴 레드(Neutral Red), 에반스 블루(Evan's Blue), 아닐린 블루(Aniline Blue)를 사용한다.The reagent preparation step (S20) is a step of preparing a reagent for dyeing the ship's ballast water prepared in the preparation step (S10), and the types of reagents used are Neutral Red, Evan's Blue, and aniline. Use Blue (Aniline Blue).
도 2를 참조하면, 해수 내 수상생물을 처리하지 않은 해수를 뉴트럴 레드(Neutral Red)로 염색시킨 것을 나타낸 사진으로, 동물성 미생물(플랑크톤)이 붉은색으로 염색된 것을 알 수 있다. Referring to FIG. 2, it is a photograph showing that seawater that has not been treated with aquatic organisms in seawater is stained with Neutral Red, and it can be seen that animal microbes (Plankton) are dyed red.
뉴트럴 레드(Neutral Red)는 동물성 미생물(플랑크톤)을 염색할 때 쓰는 시약으로, 뉴트럴 레드(Neutral Red)를 단독으로 하여 해수를 염색시키게 되면 해수 내에 존재하는 동물성 세포가 붉은 색으로 염색이 된다. 뉴트럴 레드(Neutral Red)는 비확산에 의해 세포막을 통과하여 리소좀 안에 축적되는 양이온성 염색시약이다. 상해입지 않은 세포의 리소좀에 흡수된 뉴트럴 레드 염료(Neutral Red Dye)가 누적되면서 동물성 세포가 염색이 된다.Neutral Red is a reagent used to stain animal microbes (plankton). When Neutral Red is used alone to dye seawater, animal cells present in the seawater are stained in red. Neutral Red is a cationic dye reagent that accumulates in lysosomes through the cell membrane by non-diffusion. Animal cells are stained as the Neutral Red Dye absorbed in the lysosomes of uninjured cells accumulates.
도 3을 참조하면, 해수 내 수상생물을 처리하지 않은 해수를 에반스 블루(Evan's Blue)로 염색시킨 것을 나타낸 사진으로, 죽은 식물성 미생물(플랑크톤)이 푸른색으로 염색된 것을 알 수 있다. Referring to Figure 3, a picture showing that the seawater that is not treated with aquatic organisms in seawater is stained with Evan's Blue, and it can be seen that the dead vegetative microorganism (Plankton) was dyed blue.
에반스 블루(Evan's Blue)는 죽은 식물성 미생물(플랑크톤)을 염색할 때 쓰는 시약으로, 에반스 블루(Evan's Blue)를 단독으로 하여 해수를 염색시키게 되면, 해수 내에 존재하는 죽은 식물성 세포가 푸른 색으로 염색이 된다.Evan's Blue is a reagent used to dye dead phytomicrobes (plankton). When Evan's Blue is used to dye seawater alone, dead plant cells present in the seawater are stained in blue. do.
본원발명의 시약 제조 단계(S20)의 시약은 2가지 혼합 시약 중 택일적으로 실시할 수 있다.The reagent of the reagent preparation step (S20) of the present invention may be alternatively carried out among two mixed reagents.
첫번째로, 시약 투입 단계(S30)의 투입되는 시약은 뉴트럴 레드(Neutral Red) 1.65g, 에반스 블루(Evan's Blue) 0.5g, 증류수 1000mL 비율로 혼합되도록 한다.First, the reagent to be injected in the reagent injection step (S30) is Neutral Red (Neutral Red) 1.65g, Evans Blue (Evan's Blue) 0.5g, to be mixed at a rate of 1000mL distilled water.
도 4를 참조하면, 뉴트럴 레드(Neutral Red)와 에반스 블루(Evan's Blue)가 포함된 혼합 시약으로 선박 평형수(처리 전)를 염색한 것을 나타낸 것으로 죽은 식물성 플랑크톤과 동물성 플랑크톤이 염색된 것을 확인할 수 있다.Referring to Figure 4, Neutral Red (Neutral Red) and Evans Blue (Evan's Blue) as a mixed reagent, showing that the vessels were stained with ballast water (before treatment) and confirmed that dyed phytoplankton and animal plankton were dyed. have.
또한, 도 5를 참조하면, 20분 처리한 선박 평형수에 혼합 시약으로 염색한 것을 나타낸 것으로 죽은 식물성 플랑크톤이 더 증가한 것을 확인할 수 있다.In addition, referring to Figure 5, it can be seen that the dyed phytoplankton increased by showing the dyeing with a mixed reagent in the ballast water treated for 20 minutes.
또한, 도 6을 참조하면, 80분 처리한 선박 평형수에 혼합 시약으로 염색한 것을 나타낸 것으로 죽은 식물성 플랑크톤이 보다 더 증가함은 물론 처리 시간이 더 길수록 살균 효과가 크다는 것을 확인할 수 있다.In addition, referring to FIG. 6, it was shown that the vessel was treated with a mixed reagent in a ballast water treated with 80 minutes, and the dead phytoplankton increased more and the longer the treatment time, the greater the sterilization effect.
또한, 상기의 혼합 비율은 각기 다른 색을 내는 뉴트럴 레드(Neutral Red)와 에반스 블루(Evan's Blue)가 중합반응에 의하여 미세 파티클을 형성하는 것을 최대한 방지함에 따라, 염색 시 염색약끼리 뭉친 부분과 동물성 플랑크톤이 염색된 부분, 식물성 플랑크톤이 염색된 부분을 구분할 수 있도록 한다.In addition, the mixing ratio of the neutral red (Neutral Red) and Evans Blue (Evan's Blue) giving different colors to prevent the formation of fine particles by the polymerization reaction as much as possible, when dyeing, the dyeing agent clumps and animal plankton This dyed part, phytoplankton, helps to distinguish the dyed part.
두번째는, 뉴트럴 레드(Neutral Red) 1.65g, 아닐린 블루(Aniline Blue) 10g, 증류수 1000mL 비율로 혼합되도록 한다.The second, Neutral Red (Neutral Red) 1.65g, Aniline Blue (Aniline Blue) 10g, to be mixed in a ratio of 1000mL distilled water.
도 7을 참조하면, 뉴트럴 레드(Neutral Red)와 아닐린 블루(Aniline Blue)가 포함된 혼합 시약으로 선박 평형수(처리 전)를 염색한 것을 나타낸 것으로 죽은 식물성 플랑크톤과 동물성 플랑크톤이 염색된 것을 확인할 수 있다.Referring to Figure 7, Neutral Red (Neutral Red) and aniline blue (Aniline Blue) showing the staining of the ship's ballast water (before treatment) with a mixed reagent, it was confirmed that the dead phytoplankton and animal plankton were dyed. have.
또한, 도 8을 참조하면, 20분 처리한 선박 평형수에 혼합 시약으로 염색한 것을 나타낸 것으로 죽은 식물성 플랑크톤이 더 증가한 것을 확인할 수 있다.In addition, referring to FIG. 8, it can be seen that the phytoplankton that died was further increased as it was stained with a mixing reagent in ballast water treated for 20 minutes.
또한, 도 9를 참조하면, 80분 처리한 선박 평형수에 혼합 시약으로 염색한 것을 나타낸 것으로 죽은 식물성 플랑크톤이 보다 더 증가함은 물론 처리 시간이 더 길수록 살균 효과가 크다는 것을 확인할 수 있다.In addition, referring to FIG. 9, it was shown that the vessel was treated with a mixed reagent in a ballast water treated with 80 minutes. As a result, the dead phytoplankton increased more and the longer the treatment time, the greater the sterilization effect.
또한, 상기의 혼합 비율은 각기 다른 색을 내는 뉴트럴 레드(Neutral Red)와 아닐린 블루(Aniline Blue)가 중합반응에 의하여 미세 파티클을 형성하는 것을 최대한 방지함에 따라, 염색 시 염색약끼리 뭉친 부분과 동물성 플랑크톤이 염색된 부분, 식물성 플랑크톤이 염색된 부분을 구분할 수 있도록 한다.In addition, the mixing ratio of the neutral red (Neutral Red) and aniline blue (Aniline Blue) that give different colors to prevent the formation of fine particles by the polymerization reaction as much as possible, the dyeing during the dyeing and the animal plankton This dyed part, phytoplankton, helps to distinguish the dyed part.
상기 두 시약 중 어느 하나의 혼합 시약을 택일적으로 실시할 수 있음은 물론이다.Needless to say, a mixed reagent of either of the two reagents may be alternatively carried out.
시약 투입 단계(S30)는 선박 평형수 처리수가 담긴 반응 용기에 시약 제조 단계(S20)에서 제조된 시약을 투입하는 단계이다.Reagent input step (S30) is a step of injecting the reagent prepared in the reagent preparation step (S20) into the vessel containing the ballast water treatment water.
교반 단계(S40)는 시약 투입 단계(S30)에서 시약이 투입된 선박 평형수 처리수를 일정 시간 교반을 수행하는 단계로 시약을 희석하여 반응도를 높이기 위함이다.The agitation step (S40) is a step of performing agitation of the vessel's ballast water to which the reagent is added in the reagent introduction step (S30) for a certain time to dilute the reagent to increase the reactivity.
제1 검사 단계(S50)는 시약이 투입되어 반응된 선박 평형수 처리수를 확대경으로 죽은 식물성 플랑크톤과 동물성 플랑크톤의 분포를 검사하는 단계이다.The first inspection step (S50) is a step of inspecting the distribution of dead phytoplankton and animal plankton by using a magnifying glass in the vessel ballast water treated with reagents.
또한, 도 2 내지 도 9를 참조하면, 제1 검사 단계(S50)에서 검사한 결과를 참조할 수 있다.Also, referring to FIGS. 2 to 9, the result of the inspection in the first inspection step S50 may be referred to.
또한, 교반 단계(S40)는 시약 투입 단계(S30)에서 투입된 시약과 반응할 선박 평형수 처리수의 반응도를 높이기 위해 유체에 열 에너지를 공급하도록 한다.In addition, the stirring step (S40) is to supply heat energy to the fluid to increase the reactivity of the ballast water treatment water to react with the reagents input in the reagent input step (S30).
또한, 교반 단계(S40)는 유체에 열 에너지를 공급 시 특정 온도 범위를 유지하기 위해 온도를 센싱하여 열 에너지 공급을 조절할 수도 있도록 한다.In addition, the agitation step (S40) is also possible to adjust the heat energy supply by sensing the temperature to maintain a specific temperature range when supplying heat energy to the fluid.
제2 검사 단계(S60)는 광원공급을 통해 비전 카메라 검사를 수행하여 살아있는 식물성 플랑크톤을 분포를 검사하는 단계이다.The second inspection step (S60) is a step of inspecting the distribution of living phytoplankton by performing a vision camera inspection through supply of a light source.
이상의 설명은 본 발명의 기술 사상을 예시적으로 설명한 것에 불과한 것으로서, 본 발명이 속하는 기술 분야에서 통상의 지식을 가진 자라면 본 발명의 본질적인 특성에서 벗어나지 않는 범위 내에서 다양한 수정, 변경 및 치환이 가능할 것이다. 따라서, 본 발명에 개시된 실시예 및 첨부된 도면들은 본 발명의 기술 사상을 한정하기 위한 것이 아니라 설명하기 위한 것이고, 이러한 실시예 및 첨부된 도면에 의하여 본 발명의 기술 사상의 범위가 한정되는 것은 아니다. 본 발명의 보호 범위는 아래의 청구범위에 의하여 해석되어야 하며, 그와 동등한 범위 내에 있는 모든 기술 사상은 본 발명의 권리범위에 포함되는 것으로 해석되어야 할 것이다.The above description is merely illustrative of the technical spirit of the present invention, and those of ordinary skill in the art to which the present invention pertains may make various modifications, changes, and substitutions without departing from the essential characteristics of the present invention. will be. Therefore, the embodiments and the accompanying drawings disclosed in the present invention are not intended to limit the technical spirit of the present invention, but to explain the scope of the technical spirit of the present invention. . The scope of protection of the present invention should be interpreted by the following claims, and all technical spirits within the equivalent range should be interpreted as being included in the scope of the present invention.
S10 - 준비 단계
S20 - 시약 제조 단계
S30 - 시약 투입 단계
S40 - 교반 단계
S50 - 제1 검사 단계
S60 - 제2 검사 단계
S100 - 염색 혼합시약을 이용한 처리된 선박 평형수 검사방법S10-preparatory stage
S20-reagent preparation step
S30-reagent input step
S40-Stirring step
S50-first inspection stage
S60-second inspection stage
S100-Test method for ballast water in treated vessels using a mixed reagent
Claims (4)
준비 단계에서 준비된 선박 평형수 처리수를 염색시킬 시약을 제조하는 시약 제조 단계;
선박 평형수 처리수가 담긴 반응 용기에 시약 제조 단계에서 제조된 시약을 투입하는 시약 투입 단계;
시약 투입 단계에서 시약이 투입된 선박 평형수 처리수를 일정 시간 교반을 수행하는 교반 단계;
시약이 투입되어 반응된 선박 평형수 처리수를 확대경으로 죽은 식물성 플랑크톤과 동물성 플랑크톤의 분포를 검사하는 제1 검사 단계; 및
광원공급을 통해 비전 카메라 검사를 수행하여 살아있는 식물성 플랑크톤을 분포를 검사하는 제2 검사 단계
를 포함하고,
교반 단계는
시약 투입 단계에서 투입된 시약과 반응할 선박 평형수 처리수의 반응도를 높이기 위해 유체에 열 에너지를 공급하는 것
을 포함하고
시약 투입 단계의 투입되는 시약은
뉴트럴 레드(Neutral Red) 1.65g, 에반스 블루(Evan's Blue) 0.5g, 증류수 1000mL 비율로 혼합된 것
을 포함하는 염색 혼합시약을 이용한 처리된 선박 평형수 검사방법.A preparation step of preparing the ballast water treated vessel and placing it in a reaction vessel;
A reagent preparation step of preparing a reagent for staining the treated ballast water prepared in the preparation step;
A reagent input step of inputting the reagent prepared in the reagent preparation step into the reaction vessel containing the ballast water treatment vessel;
A stirring step of performing agitation of the ballast water treated with the reagent for a predetermined time in the reagent input step;
A first inspection step of inspecting the distribution of phytoplankton and animal plankton dead by using a magnifying glass in the vessel ballast treated water reacted with reagent; And
Second inspection step to inspect the distribution of living phytoplankton by performing vision camera inspection through supply of light source
Including,
Stirring step
Supplying thermal energy to the fluid to increase the reactivity of the vessel's ballast water to react with the reagents input in the reagent input stage
And
The reagent to be injected in the reagent injection step
Neutral Red 1.65g, Evans Blue 0.5g, distilled water mixed in a ratio of 1000mL
Ballast water inspection method of the treated vessel using a dye mixed reagent containing a.
준비 단계에서 준비된 선박 평형수 처리수를 염색시킬 시약을 제조하는 시약 제조 단계;
선박 평형수 처리수가 담긴 반응 용기에 시약 제조 단계에서 제조된 시약을 투입하는 시약 투입 단계;
시약 투입 단계에서 시약이 투입된 선박 평형수 처리수를 일정 시간 교반을 수행하는 교반 단계;
시약이 투입되어 반응된 선박 평형수 처리수를 확대경으로 죽은 식물성 플랑크톤과 동물성 플랑크톤의 분포를 검사하는 제1 검사 단계; 및
광원공급을 통해 비전 카메라 검사를 수행하여 살아있는 식물성 플랑크톤을 분포를 검사하는 제2 검사 단계
를 포함하고,
교반 단계는
시약 투입 단계에서 투입된 시약과 반응할 선박 평형수 처리수의 반응도를 높이기 위해 유체에 열 에너지를 공급하는 것
을 포함하며,
시약 투입 단계의 투입되는 시약은
뉴트럴 레드(Neutral Red) 1.65g, 아닐린 블루(Aniline Blue) 10g, 증류수 1000mL 비율로 혼합된 것
을 포함하는 염색 혼합시약을 이용한 처리된 선박 평형수 검사방법.A preparation step of preparing the ballast water treated vessel and placing it in a reaction vessel;
A reagent preparation step of preparing a reagent for staining the treated ballast water prepared in the preparation step;
A reagent input step of inputting the reagent prepared in the reagent preparation step into the reaction vessel containing the ballast water treatment vessel;
A stirring step of performing agitation of the ballast water treated with the reagent for a predetermined time in the reagent input step;
A first inspection step of inspecting the distribution of phytoplankton and animal plankton dead by using a magnifying glass in the vessel ballast treated water reacted with reagent; And
Second inspection step to inspect the distribution of living phytoplankton by performing vision camera inspection through supply of light source
Including,
Stirring step
Supplying thermal energy to the fluid to increase the reactivity of the vessel's ballast water to react with the reagents input in the reagent input stage
It includes,
The reagent to be injected in the reagent injection step
Neutral Red 1.65g, Aniline Blue 10g, distilled water mixed in a ratio of 1000mL
Ballast water inspection method of the treated vessel using a dye mixed reagent containing a.
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| KR20160011672A (en) * | 2013-05-29 | 2016-02-01 | 가부시끼가이샤 사따께 | Microorganism testing method |
| KR101755117B1 (en) * | 2015-09-30 | 2017-07-07 | (주)제이엠이엔비 | Apparatus for examining survival plankton |
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| KR20160011672A (en) * | 2013-05-29 | 2016-02-01 | 가부시끼가이샤 사따께 | Microorganism testing method |
| KR101755117B1 (en) * | 2015-09-30 | 2017-07-07 | (주)제이엠이엔비 | Apparatus for examining survival plankton |
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