KR102096721B1 - Low―toxic and eco-friendly composition for controlling Dermanyssus gallinae having Oxalic Acid and method for manufacturing the same - Google Patents
Low―toxic and eco-friendly composition for controlling Dermanyssus gallinae having Oxalic Acid and method for manufacturing the same Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
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- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/30—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests characterised by the surfactants
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Abstract
Description
본 발명은 옥살산을 이용한 저독성의 친환경 닭 진드기 방제조성물 및 이의 제조방법에 관한 것으로서, 더욱 상세하게는 닭 진드기 방제효과가 우수하고, 식용란에 직접 살포되더라도 안전하며, 식용란 난각에 잔류된 살모넬라균에도 살균효과가 있는 저독성의 친환경 닭 진드기 방제조성물 및 이의 제조방법에 관한 것이다.The present invention relates to a low-toxic eco-friendly chicken mite control composition using oxalic acid and a method for manufacturing the same, more specifically, it has an excellent chicken mite control effect, is safe even when sprayed directly onto an edible egg, and is also sterilized by Salmonella remaining in the egg shell. It relates to a low-toxic eco-friendly chicken mite control composition having an effect and a method for manufacturing the same.
닭 진드기(Dermanyssus gallinae De Geer; Acaridae, 와구모)는 거미강 진드기목에 해당하며, 0.7~1.0mm의 크기로 닭, 야생조류를 비롯한 설치류에 기생하여 흡혈하는 야행성 해충으로, 닭 진드기에 물린 닭은 빈혈이나 가려움 등 스트레스로 인해 산란률이 떨어지거나 심지어는 집단 폐사를 유발할 수 있어, 양계장에 경제적 손실을 크게 일으키는 흡혈성 기생충이다. 농촌진흥청 국립축산과학원 가금연구소는 국내 양계농가의 94%가 닭진드기 감염문제를 가지고 있는 것으로 보고 있다.The chicken mite ( Dermanyssus gallinae De Geer; Acaridae) corresponds to the arachnid mite, and is a nocturnal pest that parasites and vampires chickens, wild birds and rodents with a size of 0.7 to 1.0 mm. Silver is a vampire parasite that can cause economic loss to poultry farms, as it can cause spawning rates or even group death due to stress, such as anemia or itching. The National Institute for Livestock Science, Poultry Research Institute of the Rural Development Administration estimates that 94% of domestic poultry farms have chicken mite infection.
닭 진드기가 알에서 성충이 되기까지의 1 생활사 주기는 보통 7~10일정도 소요되며, 이들의 생활사 중 흡혈을 하는 단계는 대게 부화한 후 3~5일 정도 후인 약충단계와 성충 단계이다. 닭 진드기는 한번에 4~8개의 알을 낳고 평균 5~6번 정도를 산란하므로, 생활사가 짧은 닭 진드기의 특성상 제 때에 효율적으로 방제하지 않으면 증식속도가 따라갈 수 없을 만큼 빠르게 기하급수적으로 진행될 수 있다.The life cycle from chicken mite to adulthood usually takes about 7 to 10 days, and the stage of vampire in their life cycle is usually the larvae stage and adult stage, about 3 to 5 days after hatching. Chicken mites lay 4 to 8 eggs at a time and spawn on average 5 to 6 times, so if they are not efficiently controlled in time due to the characteristics of short chicken mites, their growth rate can progress exponentially rapidly.
양계농가에서는 닭 진드기의 살충 효율을 높이기 위해서, 닭 진드기 방제용도가 아닌 성분의 안전성이 검증되지 않은 화학살충제들이 무분별하게 사용되고 있으며, 음성적으로 농작물의 해충을 억제하는 유기합성농약 방제제도 사용되고 있다.In order to increase the insecticidal efficiency of chicken mites, poultry farms use indiscriminately chemical pesticides that have not been tested for the safety of ingredients that are not intended to control chicken mites, and organic synthetic pesticide control agents that negatively suppress pests in crops are also used.
또한, 원칙적으로 닭이나 계란이 없는 빈 계사에 닭 진드기 방제제를 살포해야 하지만, 산란계 농가에서는 몇 만 마리의 닭을 일거에 비우기 쉽지 않아, 산란계와 식용란을 그대로 두고 화학살충제를 살포하는 경우가 많다고 알려져 있다. 그 과정에서 인체에 유해한 화학살충제 성분이 닭의 체내로 흡수되거나 계란 표면에 노출되면서 잔류하기도 한다. 또한 대부분의 화학살충제는 지속적으로 적용 시 내성문제로 인해 저항성이 계속 커질 수 있고, 신경세포막의 신호전달을 차단하는 신경독을 가지고 있어, 식품위생, 농약잔류, 환경오염, 작업자의 독성 문제로 닭 사육 중에 사용할 수 있는 품목은 제한적이라는 문제점이 있었다.In addition, in principle, chicken mite control agents should be applied to empty chickens without chickens or eggs, but it is not easy to empty tens of thousands of chickens at a time in laying farms, and chemical pesticides are often sprayed with the laying eggs and edible eggs intact. Is known. In the process, chemical pesticide components harmful to the human body may be absorbed into the body of the chicken or left on the surface of the egg. In addition, most chemical pesticides can continue to increase resistance due to resistance problems when applied continuously, and have neurotoxins that block the signaling of nerve cell membranes, raising chickens due to food hygiene, pesticide residues, environmental pollution, and worker toxicity problems. There was a problem that the items that can be used are limited.
실제로 지난 2017년 8월에 농림축산식품부가 국내 1,239개 산란계 농장을 전수검사한 결과, 약 52개의 일반농장과 친환경 농장에서 사용이 금지되거나 기준치를 넘어선 살충제 성분이 검출되어 부적합 판정을 받았다. 검출된 살충제 성분으로는 피프로닐, 비펜트린, 플루테녹수론, 에톡사졸 및 피리다벤 등이 있었으며, 부적합 판정을 받은 농가의 모든 계란이 출하 중지, 전량 폐기되었다. 농가에서 검출된 일부 성분들은 발암 가능성이 있는 물질로 분류되거나 다량 섭취 시 간, 갑상선, 신장 등에 이상을 일으킬 수 있다는 연구결과가 있었다. 이에 소비자들의 불안은 팽배해졌고, 믿고 먹을 수 있는 안전한 먹거리가 절실하게 요구되고 있는 실정이다.In fact, in August 2017, the Ministry of Agriculture, Food and Rural Affairs conducted a total inspection of 1,239 laying farms in Korea. As a result, pesticide ingredients that were prohibited or exceeded the standard were detected in approximately 52 general farms and eco-friendly farms, and were judged to be unsuitable. The pesticide components detected were fipronil, bifenthrin, flutenoxuron, ethoxazole, and pyridaben, and all eggs of farmers who were judged to be non-conforming were discontinued from shipment and discarded in full. Studies have shown that some of the components detected in farmhouses are classified as potentially carcinogenic substances or may cause abnormalities in the liver, thyroid, and kidneys when consumed in large quantities. As a result, consumers' anxiety has increased, and there is an urgent need for safe food that can be trusted and eaten.
이런 화학살충제의 단점을 보완하기 위해 다양한 친환경적인 재료를 이용한 병해충 방제에 대한 연구가 활발히 진행되고 있으며, 특히 유기산과 몇몇의 식물추출물이 살충효과를 나타낸다고 알려져 있다.In order to compensate for the shortcomings of such chemical pesticides, research on pest control using various eco-friendly materials is actively conducted, and it is known that organic acids and some plant extracts exhibit insecticidal effects.
대한민국 등록특허 10-1156037에서는 유기산과 식물정유를 주성분으로 한 친환경 꿀벌 병해충 방제용 훈증제를 기재하고 있으며, 유기산은 옥살산, 초산, 젖산, 숙신산, 타르타르산 및 말릭산을 사용하고 식물 정유는 티몰, 멘톨, 시네올, 리나룰, 유제놀 및 제라니올을 유효성분으로 포함하는 친환경 꿀벌 병해충 방제용 훈증제를 개시하고 있다.Republic of Korea Patent No. 10-1156037 discloses an eco-friendly fumigant for controlling pests of bees, which is composed mainly of organic acids and vegetable essential oils. Organic acids include oxalic acid, acetic acid, lactic acid, succinic acid, tartaric acid and malic acid, and the plant essential oil is thymol, menthol, It discloses an eco-friendly fumigant for controlling bee pests, which contains cineol, linarul, eugenol, and geraniol as active ingredients.
또한, 이외에도 양계에서 인체에 유해할 수 있는 성분 중 하나는 티푸스성 질환을 일으키고 식중독의 원인이 되기도 하는 살모넬라균(Salmonella spp.)이다. 살모넬라균은 다양한 원인으로 난각에 잔류되거나 식용란 내부로 침투되며 급성장염, 장티푸스 혹은 식중독 등을 일으키는 것으로 알려져 있다. 최근 2018년 8월, 계란껍질에 묻어있던 살모넬라균에 의해 오염된 제품이 학교 급식소로 유통되어 2,207명의 집단 식중독이 발생되었다.In addition, one of the components that may be harmful to the human body in poultry is Salmonella spp. , Which causes typhoid disease and causes food poisoning. Salmonella bacteria are known to remain in the eggshell for various reasons or penetrate into the edible egg and cause acute enteritis, typhoid fever, or food poisoning. In August 2018, 2,207 group food poisonings occurred as products contaminated by Salmonella in egg shells were distributed to school meals.
이에 따라 화학살충제의 단점을 보완하여 식용란에 직접 살포하더라도 안전하며, 식용란 난각에 잔류된 살모넬라균에도 살균효과가 있는 닭 진드기 방제조성물의 개발이 요구되고 있다.Accordingly, there is a need to develop a chicken mite control composition that is safe even if it is applied directly to the edible egg by supplementing the disadvantages of the chemical pesticide and has a bactericidal effect against Salmonella remaining in the egg shell.
본 발명은 상기와 같은 요구에 의해 발명된 것으로, 양계에 서식하며 동물의 체중감소, 산란율 저하, 스트레스 증대, 질병 전파와 같은 문제를 야기시키는 닭 진드기(와구모)에 대한 구제 효율이 우수한 옥살산을 이용한 방제조성물 및 이의 제조방법을 제공한다.The present invention was invented by the above-mentioned demands, and oxalic acid inhabiting poultry has excellent remedy for chicken mites (wagumo) that cause problems such as weight loss, decreased egg production, increased stress, and disease transmission in animals. Provided is a control composition used and a method for manufacturing the same.
동시에, 식용란에 직접 살포하더라도 안전하고, 식용란 난각에 잔류된 유해 살모넬라균에도 살균효과가 있는 옥살산을 이용한 저독성의 친환경 닭 진드기 방제조성물 및 이의 제조방법을 제공한다.At the same time, it is safe to spray directly onto the edible egg, and provides a low-toxic eco-friendly chicken mite control composition using oxalic acid which has a bactericidal effect against harmful salmonella bacteria remaining in the eggshell and a method for manufacturing the same.
본 발명은 상기와 같은 목적을 달성하기 위하여 옥살산 및 용매로 이루어지는 것을 특징으로 하는 옥살산을 이용한 저독성의 친환경 닭 진드기 방제조성물 및 이의 제조방법을 제공한다.The present invention provides a low-toxic eco-friendly chicken mite control composition using oxalic acid and a method of manufacturing the same, and a method for manufacturing the same, comprising oxalic acid and a solvent to achieve the above object.
본 발명자들은 닭 진드기를 효과적으로 방제하면서도 식용란 난각에 잔류된 유해 살모넬라균에도 효과가 있는 저독성의 친환경 닭 진드기 방제조성물에 대하여 연구한 결과, 닭 진드기 방제 효과를 나타내며 식용란에 직접 살포하더라도 안전한 옥살산의 사용 농도범위를 알아내었고, 옥살산의 닭 진드기 방제 효율을 우수하게 증진시키면서 동시에 식용란 난각에 잔류된 유해 살모넬라균에도 효과가 있는 계면활성제와 첨가 농도범위를 정하여 본 발명을 완성하였다.The present inventors have researched on a low-toxic eco-friendly chicken mite control composition that effectively controls chicken mites, but is also effective against harmful salmonella bacteria remaining in the eggshell of the edible egg. As a result, the concentration of use of oxalic acid, which shows a chicken mite control effect and is safe even when sprayed directly onto the edible egg The range was determined, and the present invention was completed by determining a surfactant and an added concentration range effective for harmful salmonella bacteria remaining in the edible egg shell while simultaneously improving the control efficiency of chicken mite of oxalic acid.
본 발명의 닭 진드기 방제조성물은 집먼지진드기, 털진드기, 닭진드기 및 귀진드기로 이루어진 군으로부터 선택되는 어느 하나 이상에 대한 방제제인 것이 바람직하며, 구체적으로 닭 진드기 방제조성물인 것이 보다 바람직하나 이에 한정되지 않는다.The chicken mite control composition of the present invention is preferably a control agent for any one or more selected from the group consisting of house dust mite, hair mite, chicken mite and ear mite, and more specifically, it is more preferably a mite control composition, but is not limited thereto. Does not.
상기 닭 진드기 방제조성물은 옥살산 2.1 내지 3.9 중량%와 잔량의 용매가 혼합되어 이루어지는 것을 특징으로 하는 옥살산을 이용한 저독성의 친환경 닭 진드기 방제조성물을 제공한다.The chicken mite control composition provides an eco-friendly chicken mite control composition of low toxicity using oxalic acid, characterized in that 2.1 to 3.9% by weight of oxalic acid and a residual amount of solvent are mixed.
상기 용매는 물, 에탄올, 메탄올, 에틸아세테이트, 아세톤, N-메칠피롤리돈, 디메칠설폭사이드 및 디메칠포름아마이드로 구성된 군으로부터 하나 또는 둘 이상 선택될 수 있다.The solvent may be selected from the group consisting of water, ethanol, methanol, ethyl acetate, acetone, N-methylpyrrolidone, dimethyl sulfoxide, and di methyl formamide.
또한, 옥살산의 닭 진드기 방제 효율을 증진시키고 식용란 난각에 잔류된 유해 살모넬라균을 살균하기 위해 계면활성제 0.1 내지 3 중량%가 더 포함될 수 있다.In addition, 0.1 to 3% by weight of surfactant may be further included to improve the control efficiency of chicken mite of oxalic acid and to sterilize harmful salmonella bacteria remaining in the edible egg shell.
상기 계면할성제는 모노이소트리데실포스페이트, 디이소트리데실포스페이트, 폴리옥시에틸렌옥틸페닐에테르, 폴리옥시에틸렌트리데실에테르 및 폴리옥시에틸렌에틸헥실에테르로 구성된 군으로부터 하나 또는 둘 이상 선택될 수 있다.The surfactant may be selected from one or more of the group consisting of monoisotridecyl phosphate, diisotridecyl phosphate, polyoxyethylene octylphenyl ether, polyoxyethylene tridecyl ether and polyoxyethylene ethylhexyl ether.
상기 닭 진드기 방제조성물에는 필요에 따라서 접착제, 분산제, 안정화제 및 보존제 등이 첨가될 수 있다.Adhesives, dispersants, stabilizers and preservatives may be added to the chicken mite control composition, if necessary.
본 발명에 따른 옥살산을 이용한 저독성의 친환경 닭 진드기 방제조성물은 저독성으로 식용란에 직접 살포되더라도 안전하여 약해가 적은 장점을 가진다. 뿐만 아니라, 유기합성 농약 또는 인공화학 살충제가 아닌 친환경적인 소재를 사용하기 때문에 내성문제, 농약 성분의 환경 잔류문제 등이 발생하지 않는 장점도 가진다. 또한, 닭 진드기 구제 효율도 우수하여 산란계의 스트레스를 감소시키고 산란율을 증가시키며, 나아가 식용란 난각에 잔류된 유해 살모넬라균에도 살균효과가 있어, 농가 경쟁력 향상 및 소비자 신뢰회복의 효과를 기대할 수 있다.The low-toxic eco-friendly chicken mite control composition using oxalic acid according to the present invention is low-toxic and safe even when sprayed directly onto an edible egg, so it has the advantage of less damage. In addition, since it uses eco-friendly materials, not organic synthetic pesticides or artificial chemical pesticides, it has the advantage that resistance problems and environmental residues of pesticide components do not occur. In addition, chicken mite control efficiency is also excellent, which reduces the stress of the spawning system, increases the spawning rate, and further has a bactericidal effect on the harmful Salmonella bacteria remaining in the edible egg shell, which can be expected to improve farming competitiveness and restore consumer confidence.
이하, 본 발명에 따른 옥살산을 이용한 저독성의 친환경 닭 진드기 방제조성물 및 이의 제조방법을 하기의 실시예에 의해 상세히 설명한다. 단, 하기 실시예는 본 발명을 예시하는 것일 뿐, 본 발명의 내용이 하기 실시예에 의해 한정되는 것은 아니다.Hereinafter, a low-toxic eco-friendly chicken mite control composition using oxalic acid according to the present invention and a method of manufacturing the same will be described in detail by the following examples. However, the following examples are merely illustrative of the present invention, and the contents of the present invention are not limited by the following examples.
본 발명에 따른 옥살산(Oxalic acid)을 이용한 저독성의 친환경 닭 진드기 방제조성물은 옥살산 및 용매로 이루어지며, 옥살산 2.1~3.9 중량%와 잔량의 용매를 혼합하여 이루어지는 것이 바람직하다.The low-toxic eco-friendly chicken mite control composition using oxalic acid according to the present invention is made of oxalic acid and a solvent, and is preferably made by mixing oxalic acid 2.1 to 3.9 wt% and a residual amount of a solvent.
옥살산은 칼륨염 또는 칼슘염의 형태로 식물계에 널리 분포되어 있으며, 분자식은 C2H2O4의 가장 간단한 구조의 카르복실산으로, 녹이나 잉크 등으로 인해 생긴 얼룩을 지우는 표백제로 많이 사용된다. 상기 옥살산은 본 발명의 닭 진드기 방제조성물의 소독력을 나타내는 동시에, 닭 진드기에 살충효과를 나타낸다. 상기 옥살산의 함량이 2 중량% 이하로 함유되면, 본 발명의 닭 진드기 방제조성물의 살충효과가 저하되며, 상기 옥살산의 함량이 4 중량% 이상이면, 식용란의 난각이 탈색 및 표면탈락 현상을 보이게 된다.Oxalic acid is widely distributed in the plant system in the form of potassium salts or calcium salts, and the molecular formula is the simplest structure of carboxylic acid of C 2 H 2 O 4 , and is often used as a bleach to remove stains caused by rust or ink. The oxalic acid exhibits the disinfecting power of the chicken mite control composition of the present invention, while exhibiting a pesticidal effect on the chicken mite. If the content of the oxalic acid is 2% by weight or less, the insecticidal effect of the chicken mite control composition of the present invention is lowered, and if the content of the oxalic acid is 4% by weight or more, the egg shell of the edible egg shows discoloration and surface dropping phenomenon. .
상기 용매는 옥살산과 계면활성제를 원활히 분산시켜 희석되도록 하는 기능을 하는 것을 사용할 수 있다. 바람직하게는 물, 에탄올, 메탄올, 에틸아세테이트, 아세톤, N-메칠피롤리돈, 디메칠설폭사이드 및 디메칠포름아마이드로 구성된 군으로부터 하나 또는 둘 이상 선택될 수 있다.The solvent may be used to function to disperse the oxalic acid and surfactant smoothly to be diluted. Preferably, one or two or more may be selected from the group consisting of water, ethanol, methanol, ethyl acetate, acetone, N-methylpyrrolidone, dimethyl sulfoxide and di methyl formamide.
또한, 옥살산의 닭 진드기 방제 효율을 증진시키고 식용란 난각에 잔류된 살모넬라균을 살균하기 위해, 계면활성제 0.1 내지 3 중량%가 더 포함될 수 있다.In addition, in order to improve the control efficiency of chicken mite of oxalic acid and to sterilize Salmonella remaining in the edible eggshell, 0.1 to 3% by weight of surfactant may be further included.
상기 계면활성제는 표적 미생물 세포 표면에 흡착된 뒤 세포막을 파괴하여 미생물을 불활성화시키고, 본 발명의 닭 진드기 방제조성물의 살충성분을 처리표면에 확산시키는 역할을 한다. 상기 계면활성제의 함량이 0.1 중량% 미만으로 함유되면, 본 발명의 닭 진드기 방제조성물의 표면 확산효과가 저하되며, 상기 계면황성제의 함량이 3 중량% 초과이면, 표면 확산효과는 더 이상 향상되지 않고, 옥살산의 상대적인 함량이 줄어들어 방제조성물의 살충효과가 저하된다.The surfactant is adsorbed to the target microbial cell surface and then destroys the cell membrane to inactivate the microorganism and serves to diffuse the insecticidal component of the chicken mite control composition of the present invention to the treated surface. When the content of the surfactant is less than 0.1% by weight, the surface diffusion effect of the chicken mite control composition of the present invention is lowered, and when the content of the surfactant is more than 3% by weight, the surface diffusion effect is no longer improved. , The relative content of oxalic acid decreases, and the insecticidal effect of the control composition decreases.
상기 계면활성제는 옥살산과 혼합 시 닭 진드기 방제효율이 가장 뛰어나고, 식용란 난각에 잔류된 살모넬라균에도 살균효과가 우수한 물질을 사용하였으며, 구체적으로는 모노이소트리데실포스페이트, 디이소트리데실포스페이트, 폴리옥시에틸렌옥틸페닐에테르, 폴리옥시에틸렌트리데실에테르 및 폴리옥시에틸렌에틸헥실에테르로 구성된 군으로부터 하나 또는 둘 이상 선택될 수 있다.The surfactant has the best control efficiency against chicken mites when mixed with oxalic acid, and a material having excellent sterilizing effect against Salmonella remaining in the egg shell of the edible egg was used. Specifically, monoisotridecyl phosphate, diisotridecyl phosphate, polyoxy One or two or more may be selected from the group consisting of ethylene octyl phenyl ether, polyoxyethylene tridecyl ether and polyoxyethylene ethyl hexyl ether.
또한, 닭 진드기 방제효과를 더욱 더 증진시키기 위해서는, 본 발명의 닭 진드기 방제조성물에 여뀌 추출물 및/또는 때죽나무 추출물을 혼합하여 사용할 수 있다.In addition, in order to further enhance the control effect of chicken mite, the chicken mite control composition of the present invention can be used by mixing the extract and / or the extract from the hedgehog tree.
여뀌(Persicaria hydropiper. L.)은 북반구 온대 및 난대지방에 걸쳐 분포하는 마디풀목 마디풀과의 1년생 초본으로 흔히 습지 또는 물가에서 야생한다. 여뀌의 건조한 잎과 줄기를 깔면 이나 벼룩이 없어진다고 전해지며, 충과 균에 대해 기피, 살충효과가 있는 것으로 알려져 있다. Yeo Qu ( Persicaria hydropiper. L.) is a perennial herbaceous family of the nodaceae family distributed throughout the temperate and temperate regions of the northern hemisphere, and is often wild in wetlands or watersides. It is said that fleas or fleas disappear after laying dry leaves and stems of Yeo Qu, and it is known to have a repelling and insecticidal effect on insects and fungi.
여뀌를 추출하는 방법으로는 여뀌의 잎과 줄기 200g을 믹서기에서 마쇄하고, 여뀌 잎과 줄기 5배의 에탄올을 혼합하여 45~55℃에서 24시간 방치한 후에, 여과시킨 추출용액을 45~55℃에서 회전진공감압농축기(EYELA N-1000, 일본)로 농축한 후, 동결건조과정을 거쳐 파우더를 얻을 수 있다.As a method of extracting the leaves, 200 g of the leaves and stems of the leaves are ground in a blender, mixed with 5 times the ethanol of the leaves and the stems and allowed to stand at 45 to 55 ° C. for 24 hours, followed by filtering the extracted solution at 45 to 55 ° C. After concentrating with a rotary vacuum concentrator (EYELA N-1000, Japan), the powder can be obtained through lyophilization.
때죽나무(Styrax japonica)는 때죽나무과의 때죽나무목으로, 한국, 중국, 일본이 원산이며, 높이는 10m 내외로 자라고, 씨앗은 1.2~1.4cm 정도의 종모양과 비슷한 형태이다. 때죽나무의 열매와 잎은 약 4% 정도의 에고사포닌(Egosaponin)을 함유하고 있는데, 에고사포닌은 매우 독성이 강한 것으로 열매를 찧어서 짜낸 즙을 냇물에 풀어 떠오르는 물고기를 잡는 데 사용되었으며, 충과 균에 대해서도 강한 기피, 살충 효과가 있는 것으로 알려져 있다. Styrax japonica ( Styrax japonica ) is a tree of the family of the syrup, native to Korea, China, and Japan, grows up to about 10 m in height, and seeds are similar in shape to bells of about 1.2 to 1.4 cm. The fruits and leaves of the bamboo tree contain about 4% of Egosaponin, which is very toxic and used to catch floating fish by squeezing the fruit into a stream and stalking the fungus and fungus. It is also known to have strong repellent and insecticidal effects.
때죽나무를 추출하는 방법으로는 떼죽나무의 잎과 열매 200g을 믹서기에서 마쇄하고, 떼죽나무 잎과 열매 5배의 메탄올을 혼합하여 45~55℃에서 24시간 방치한 후에, 여과시킨 추출용액을 45~55℃에서 회전진공감압농축기(EYELA N-1000, 일본)로 농축한 후, 동결건조과정을 거쳐 파우더를 얻을 수 있다.As a method of extracting the dead tree, 200 g of the leaves and fruits of the bamboo tree are ground in a blender, mixed with 5 times the methanol of the leaves and the leaves of the bamboo tree, and left at 45 to 55 ° C. for 24 hours, followed by filtering the extracted solution. After concentration at ~ 55 ℃ with a rotary vacuum concentrator (EYELA N-1000, Japan), powder can be obtained through a lyophilization process.
상기 여뀌 추출물과 때죽나무 추출물은 본 발명의 닭 진드기 방제조성물에 각각 4 내지 7 중량%가 더 첨가될 수 있다.4 to 7% by weight may be further added to the chicken mite control composition of the present invention.
또한, 상기 닭 진드기 방제조성물에는 필요에 따라서 접착제, 분산제, 안정화제 및 보존제 등이 첨가될 수 있다.In addition, an adhesive, a dispersing agent, a stabilizing agent and a preservative may be added to the chicken mite control composition as necessary.
하기 실시예에 의해 제조된 닭 진드기 방제조성물의 닭 진드기 살충력을 평가하기 위하여 각각의 조성물에 대한 효력시험 측정방법은 2008년 식품의약품안전청이 고시한‘전염병예방용 살충제 등의 효력시험 가이드라인’에 따라 경기도북부 동물위생시험소에서 효력시험을 실시하였다.In order to evaluate the chicken mite pesticidal composition of the chicken mite control composition prepared by the following examples, the method for measuring the efficacy test for each composition was published in the 'Efficacy Test Guidelines for Infectious Disease Prevention Pesticides' published by the Food and Drug Administration in 2008. Accordingly, the efficacy test was conducted at the animal hygiene laboratory in northern Gyeonggi Province.
<실시예 1> 옥살산의 효능확인<Example 1> Efficacy confirmation of oxalic acid
옥살산의 효능확인 시험은 옥살산 3 중량%, 5 중량%, 7 중량% 및 10 중량%와 잔량의 용매를 혼합하였으며, 상기 실시예 1에서 용매는 불순물을 제거한 정제수가 사용되었다. 상기 실시예 1에서 제조된 조성물을 닭 진드기에 7초간 분사하고, 24시간 후 닭 진드기 살충율을 조사하였다. 각 농도마다 평균 살충율을 3회 반복하여 조사하였으며, 평균 살충율은 대조군에서 죽은 개체가 보일 경우 보정살충율을 적용해 계산하였다.In the test for confirming the efficacy of oxalic acid, 3% by weight of oxalic acid, 5% by weight, 7% by weight, and 10% by weight of solvent were mixed with a residual amount, and in Example 1, purified water from which impurities were removed was used. The composition prepared in Example 1 was sprayed on a chicken mite for 7 seconds, and the insecticidal rate of chicken mite was investigated after 24 hours. The average insecticidal rate for each concentration was investigated three times, and the average insecticidal rate was calculated by applying the corrected insecticidal rate when dead individuals were seen in the control group.
보정 살충율 = (실험군의 치사율-대조군의 치사율)/(100-대조군의 치사율)Corrected insecticide rate = (mortality rate in the experimental group-mortality rate in the control group) / (100-mortality rate in the control group)
(중량%)Treatment concentration
(weight%)
상기 표 1에서 옥살산 혼합물은 3 내지 10 중량%의 모든 농도에서 75%이상의 닭 진드기 살충효과를 나타내는 것을 확인하였다.In Table 1, it was confirmed that the oxalic acid mixture exhibited a pesticidal effect of chicken mite of 75% or more at all concentrations of 3 to 10% by weight.
<실시예 2> 옥살산 식용란에 직접 분무시 변화 시험<Example 2> Change test when sprayed directly into oxalic acid edible eggs
옥살산 식용란에 분무시 변화 시험은 옥살산 2 중량%, 3 중량%, 4 중량% 및 5 중량%와 잔량의 용매를 혼합하였으며, 상기 실시예 2에서 용매는 불순물을 제거한 정제수가 사용되었다. 상기 실시예 2에서 제조된 조성물을 식용란에 분사하고, 24시간 후 식용란의 표면 변화를 조사하였다. 각 농도마다 3회 반복하여 조사하였다.In the spray test on oxalic acid edible eggs, 2% by weight, 3% by weight, 4% by weight, and 5% by weight of oxalic acid were mixed with a residual amount of solvent, and in Example 2, purified water from which impurities were removed was used. The composition prepared in Example 2 was sprayed onto the edible egg, and the surface change of the edible egg was examined after 24 hours. It was irradiated three times for each concentration.
(중량%)Treatment concentration
(weight%)
상기 표 2에서 옥살산 혼합물은 4 중량%와 5 중량%의 농도에서 식용란 난각의 탈색 및 표면탈락 현상이 보이는 것을 확인하였으며, 4 중량% 이상의 옥살산 혼합물은 식용란에 직접 살포시 안전하지 못하다는 것을 확인하였다.In Table 2, it was confirmed that the oxalic acid mixture showed discoloration and surface dropping of the edible eggshell at concentrations of 4% by weight and 5% by weight, and it was confirmed that the oxalic acid mixture of 4% by weight or more was not safe when sprayed directly onto the edible egg.
<실시예 3> 옥살산, 용매 및 계면활성제 혼합물의 제조<Example 3> Preparation of oxalic acid, solvent and surfactant mixture
상기 옥살산 2 중량%, 3 중량%와 상기 계면활성제 3 중량% 및 잔량의 용매를 혼합하여 조성물을 제조하였다. 상기 실시예 3에서 용매는 불순물을 제거한 정제수가 사용되었고, 계면활성제는 모노이소트리데실포스페이트-디이소트리데실포스페이트 중합체, 폴리옥시에틸렌옥틸페닐에테르, 폴리옥시에틸렌트리데실에테르 및 폴리옥시에틸렌에틸헥실에테르로가 사용되었다.The composition was prepared by mixing the oxalic acid 2% by weight, 3% by weight with the surfactant 3% by weight, and the remaining amount of the solvent. In the above Example 3, purified water from which impurities were removed was used, and the surfactant was monoisotridecyl phosphate-diisotridecyl phosphate polymer, polyoxyethylene octylphenyl ether, polyoxyethylene tridecyl ether, and polyoxyethylene ethylhexyl. Ethero was used.
상기 용매, 옥살산 및 계면활성제는 교반기에 각각 차례대로 투입되어 혼합되었고, 교반시간은 50 내지 100rpm에서 15분 내지 20분 교반을 실시하는 것이 바람직하며, 교반액이 투명해지면 교반을 종료한다. 상기 교반기는 당업계에서 통상적으로 사용하는 것으로 1000L 기준 0 내지 2000rpm을 운용할 수 있는 것이면 그 종류에 한정을 두지 않는다.The solvent, oxalic acid, and surfactant were added to the stirrer in turn and mixed, and the stirring time is preferably performed for 15 minutes to 20 minutes at 50 to 100 rpm, and stirring is terminated when the stirring solution becomes transparent. The stirrer is generally used in the art, and is not limited to the type as long as it can operate from 0 to 2000 rpm based on 1000L.
<실시예 4> 닭 진드기에 대한 살충력 검정 시험<Example 4> Insecticidal assay test for chicken mites
상기 실시예 3에서 제조된 조성물을 닭 진드기에 7초간 분사하고, 24시간 후 닭 진드기 살충률을 조사하였다. 각 농도마다 평균 살충율을 3회 반복하여 조사하였으며, 대조군에서 죽은 개체가 보일 경우는 보정살충율로 보정하여 평균 상출율을 계산하였다.The composition prepared in Example 3 was sprayed on a chicken mite for 7 seconds, and the insecticidal rate of chicken mite was examined after 24 hours. The average insecticidal rate for each concentration was repeatedly examined three times, and when the dead individuals were seen in the control group, the average emergence rate was calculated by correcting the calibrated insecticide rate.
보정 살충율 = (실험군의 치사율-대조군의 치사율)/(100-대조군의 치사율)Corrected insecticide rate = (mortality rate in the experimental group-mortality rate in the control group) / (100-mortality rate in the control group)
(단위:중량%, 잔량:정제수)Treatment concentration
(Unit: weight%, residual amount: purified water)
+ 3% 모노이소트리데실포스페이트
-디이소트리데실포스페이트 중합체2% oxalic acid
+ 3% monoisotridecylphosphate
-Diisotridecylphosphate polymer
+ 3% 모노이소트리데실포스페이트
-디이소트리데실포스페이트 중합체3% oxalic acid
+ 3% monoisotridecylphosphate
-Diisotridecylphosphate polymer
+ 3% 폴리옥시에틸렌옥틸페닐에테르2% oxalic acid
+ 3% polyoxyethylene octylphenyl ether
+ 3% 폴리옥시에틸렌옥틸페닐에테르3% oxalic acid
+ 3% polyoxyethylene octylphenyl ether
+ 3% 폴리옥시에틸렌트리데실에테르2% oxalic acid
+ 3% polyoxyethylene tridecyl ether
+ 3% 폴리옥시에틸렌트리데실에테르3% oxalic acid
+ 3% polyoxyethylene tridecyl ether
+ 3% 폴리옥시에틸렌에틸헥실에테르2% oxalic acid
+ 3% polyoxyethylene ethylhexyl ether
+ 3% 폴리옥시에틸렌에틸헥실에테르3% oxalic acid
+ 3% polyoxyethylene ethylhexyl ether
상기 표 3에서 상기 2 중량%의 옥살산과 3 중량%의 계면활성제 혼합 조성물은 모두 89% 내지 93%의 닭 진드기 살충효과를 나타내었고, 상기 3 중량%의 옥살산과 3 중량%의 계면활성제 혼합 조성물은 모두 95% 이상의 우수한 닭 진드기 살충효과를 나타내는 것을 확인하였으며, 상기 계면활성제에 의한 옥살산의 닭 진드기 살충효력이 상승되었음을 확인하였다. 특히, 상기 3 중량% 옥살산과 3 중량% 폴리옥시에틸렌트리데실에테르 혼합 조성물은 평균 98.1%의 가장 탁월한 닭 진드기 살충효과를 나타내는 것을 확인하였다.In Table 3, the mixture composition of 2% by weight of oxalic acid and 3% by weight of surfactant showed all insecticidal effects of 89% to 93% of chicken mite, and the composition of 3% by weight of oxalic acid and 3% by weight of surfactant It was confirmed that all exhibited excellent chicken mite insecticidal effect of at least 95%, and that the chicken mite insecticidal efficacy of oxalic acid was increased by the surfactant. In particular, it was confirmed that the 3% by weight oxalic acid and 3% by weight polyoxyethylene tridecyl ether mixture composition showed the most excellent chicken mite insecticidal effect of 98.1% on average.
<실시예 5> 살모넬라균 살균 시험<Example 5> Salmonella bacteria sterilization test
살모넬라균(Salmonella typhimurium)은 국립수의과학검역원으로부터 분양 받아 사용하였으며, LB 액체 배지에 연속 2 내지 3회 계대배양하여 활력이 있는 세균을 사용하였다. 온도는 37℃에서 22 내지 26시간 동안 배양한 후 사용직전까지 37℃를 유지시켰다. Salmonella typhimurium was used by pre-sale from the National Veterinary Science and Quarantine Service, and active bacteria were passaged through LB liquid medium 2-3 times in a row. The temperature was incubated at 37 ° C for 22 to 26 hours, and then maintained at 37 ° C until immediately before use.
배양된 상기 살모넬라균(1×106 cfu/mL)에 상기 실시예 3에서 제조된 조성물을 각각 투여하였다. 6시간 간격으로 LB고체 평판 배지에 100μL씩 평판 도말하여 37℃에서 배양한 후 형성된 집락을 계수하여 조성물의 농도와 노출 시간에 따른 살모넬라균의 생존율을 각각 조사하였다. 각 농도마다 평균 생존율을 3회 반복하여 조사하였다.To the cultured Salmonella (1 × 10 6 cfu / mL), each of the compositions prepared in Example 3 was administered. After plating at 100 µL on LB solid plate medium at 6 hour intervals and incubating at 37 ° C, colonies formed were counted to investigate the survival rate of Salmonella according to the concentration of the composition and exposure time. The average survival rate for each concentration was examined three times.
(단위:중량%, 잔량:정제수)Treatment concentration
(Unit: weight%, residual amount: purified water)
+ 3% 모노이소트리데실포스페이트
-디이소트리데실포스페이트 중합체2% oxalic acid
+ 3% monoisotridecylphosphate
-Diisotridecylphosphate polymer
+ 3% 모노이소트리데실포스페이트
-디이소트리데실포스페이트 중합체3% oxalic acid
+ 3% monoisotridecylphosphate
-Diisotridecylphosphate polymer
+ 3% 폴리옥시에틸렌옥틸페닐에테르2% oxalic acid
+ 3% polyoxyethylene octylphenyl ether
+ 3% 폴리옥시에틸렌옥틸페닐에테르3% oxalic acid
+ 3% polyoxyethylene octylphenyl ether
+ 3% 폴리옥시에틸렌트리데실에테르2% oxalic acid
+ 3% polyoxyethylene tridecyl ether
+ 3% 폴리옥시에틸렌트리데실에테르3% oxalic acid
+ 3% polyoxyethylene tridecyl ether
+ 3% 폴리옥시에틸렌에틸헥실에테르2% oxalic acid
+ 3% polyoxyethylene ethylhexyl ether
+ 3% 폴리옥시에틸렌에틸헥실에테르3% oxalic acid
+ 3% polyoxyethylene ethylhexyl ether
상기 표 4에서 상기 2 중량%의 옥살산과 3 중량%의 계면활성제 혼합 조성물은 처리 후 18시간이 지나도 살모넬라균을 완전히 살균하는 효과를 보여주지 못한 것을 확인하였고, 상기 3 중량%의 옥살산과 3 중량%의 계면활성제 혼합 조성물은 처리 12시간 이후 완전한 살모넬라균 살균효과를 나타내는 것을 확인하였다.In Table 4, it was confirmed that the 2% by weight oxalic acid and 3% by weight surfactant mixture composition did not show the effect of completely sterilizing Salmonella even after 18 hours of treatment, and 3% by weight with the 3% by weight oxalic acid % Of the surfactant mixture composition was confirmed to exhibit complete Salmonella bactericidal effect after 12 hours of treatment.
상기 표 1 내지 4에서 나타난 바와 같이, 상기 옥살산 3중량%, 계면활성제 3 중량% 및 잔량의 정제수를 혼합한 조성물은 모두 식용란에 직접 살포시 난각의 탈색 및 표면탈락 현상을 일으키지 않아 안전하고, 95% 이상의 탁월한 닭 진드기 살충효과를 나타내며, 처리 12시간 이후 살모넬라균에 완전한 살균효과를 나타내는 닭 진드기 방제조성물의 최적의 조합임을 확인하였다.As shown in Tables 1 to 4, the composition in which the oxalic acid 3% by weight, the surfactant 3% by weight, and the remaining amount of purified water are all mixed is safe and does not cause discoloration of the egg shell and surface dropping phenomenon when sprayed directly onto the edible egg, 95% It was confirmed that it is the optimum combination of the above-mentioned chicken mite control composition, which exhibits excellent chicken mite insecticidal effect and shows complete sterilization effect on Salmonella after 12 hours of treatment.
지금까지 예시적인 실시 태양을 참조하여 본 발명을 기술하여 왔지만, 본 발명이 속하는 기술 분야의 당업자는 본 발명의 범주를 벗어나지 않고서도 다양한 변화를 실시할 수 있을 것이다. 또한, 본 발명이 본 발명을 실시하는데 계획된 최상의 양식으로서 개시된 특정 실시 태양으로 국한되는 것이 아니며, 본 발명이 첨부된 특허청구의 범위에 속하는 모든 실시 태양을 포함하는 것으로 해석되어야 한다.Although the present invention has been described with reference to exemplary embodiments, those skilled in the art to which the present invention pertains may make various changes without departing from the scope of the present invention. In addition, the invention is not to be limited to the specific embodiments disclosed as the best mode planned for practicing the invention, but it should be construed that the invention includes all embodiments falling within the scope of the appended claims.
Claims (3)
상기 닭 진드기 방제조성물의 제조방법은 용매 93.1 내지 97.8 중량%, 옥살산 2.1 내지 3.9 중량% 및 계면활성제 0.1 내지 3 중량%를 혼합하여 제조된 닭 진드기 방제조성물에 여뀌 추출물을 더 혼합하여 제조하되,
상기 여뀌 추출물의 추출방법은 여뀌의 잎과 줄기를 믹서기로 마쇄하고, 상기 여뀌 잎과 줄기 무게의 5배 중량의 에탄올을 혼합하여 45~55℃에서 24시간 방치한 후에, 여과시킨 추출용액을 45~55℃에서 회전진공감압농축기로 농축하는 방법을 사용하며,
상기 계면활성제는 모노이소트리데실포스페이트, 디이소트리데실포스페이트, 폴리옥시에틸렌옥틸페닐에테르, 폴리옥시에틸렌트리데실에테르, 폴리옥시에틸렌에틸헥실에테르로 구성된 군으로부터 선택되는 하나 또는 둘 이상의 혼합물인 것을 특징으로 하는 옥살산을 이용한 저독성의 친환경 닭 진드기 방제조성물의 제조방법
In the manufacturing method of a low-toxic eco-friendly chicken mite control composition using oxalic acid,
The method for preparing the chicken mite control composition is prepared by further mixing the extract with a chicken mite control composition prepared by mixing solvent 93.1 to 97.8% by weight, oxalic acid 2.1 to 3.9% by weight, and surfactant 0.1 to 3% by weight,
The extracting method of the extract is milled with a blender, and 5 times the weight of the leaf and stem is mixed with ethanol and left at 45 to 55 ° C for 24 hours, followed by filtering the extracted solution. It uses a method of concentrating with a rotary vacuum decompressor at ~ 55 ℃.
The surfactant is one or two or more mixtures selected from the group consisting of monoisotridecyl phosphate, diisotridecyl phosphate, polyoxyethylene octylphenyl ether, polyoxyethylene tridecyl ether, and polyoxyethylene ethylhexyl ether. Manufacturing method of low toxicity eco-friendly chicken mite control composition using oxalic acid
상기 닭 진드기 방제조성물의 제조방법은 닭 진드기 방제조성물에 혼합되는 여뀌 추출물 대신에 때죽나무 추출물을 혼합하여 제조하며,
상기 때죽나무 추출물의 추출방법은 떼죽나무의 잎과 열매를 믹서기로 마쇄하고, 상기 마쇄한 떼죽나무 잎과 열매 무게의 5배 중량의 메탄올을 혼합하여 45~55℃에서 24시간 방치한 후에, 여과시킨 추출용액을 45~55℃에서 회전진공감압농축기로 농축하는 방법을 사용하는 것을 특징으로 하는 옥살산을 이용한 저독성의 친환경 닭 진드기 방제조성물의 제조방법
According to claim 2,
The method for preparing the chicken mite control composition is prepared by mixing the extract of the tart tree instead of the extract that is mixed with the chicken mite control composition,
The extracting method of the prunus bark extract is pulverized with the blender of the leaves and berries of the prickle tree, mixed with 5 times the weight of the weight of the ground prickle leaf and the fruit, and left for 24 hours at 45 to 55 ° C., followed by filtration. Method of manufacturing a low-toxic eco-friendly chicken mite control composition using oxalic acid, characterized by using a method of concentrating the extracted solution with a rotary vacuum decompressor at 45 ~ 55 ℃.
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| KR20100108648A (en) * | 2009-03-30 | 2010-10-08 | 대한민국(농촌진흥청장) | The fumigant to control honeybee pest and diseases |
| KR20120079706A (en) * | 2011-01-05 | 2012-07-13 | 동부팜한농 주식회사 | An insecticidal composition for disinfecting stable and poultry farm |
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| KR20100108648A (en) * | 2009-03-30 | 2010-10-08 | 대한민국(농촌진흥청장) | The fumigant to control honeybee pest and diseases |
| KR20120079706A (en) * | 2011-01-05 | 2012-07-13 | 동부팜한농 주식회사 | An insecticidal composition for disinfecting stable and poultry farm |
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