KR102034178B1 - Plasma-treated pepper leaf extreact with improved skin whitening effect and manufacturing method thereof - Google Patents
Plasma-treated pepper leaf extreact with improved skin whitening effect and manufacturing method thereof Download PDFInfo
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- KR102034178B1 KR102034178B1 KR1020180007217A KR20180007217A KR102034178B1 KR 102034178 B1 KR102034178 B1 KR 102034178B1 KR 1020180007217 A KR1020180007217 A KR 1020180007217A KR 20180007217 A KR20180007217 A KR 20180007217A KR 102034178 B1 KR102034178 B1 KR 102034178B1
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- South Korea
- Prior art keywords
- pepper leaf
- leaf extract
- plasma
- red pepper
- skin whitening
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Abstract
본 발명은 피부 미백효과가 증진된 플라즈마 처리된 고춧잎 추출물 및 그의 제조방법에 관한 것으로, 플라즈마 처리된 고춧잎 추출물은 플라즈마를 처리하지 않은 고춧잎 추출물에 비해 총 폴리페놀 함량이 증가되고, 항산화 효과 및 멜라닌 생성을 억제시키는 효과가 현저히 증진되므로, 본 발명의 제조방법으로 제조된 고춧잎 추출물을 유효성분으로 함유하는 조성물은 피부 미백을 위한 기능성 화장품, 피부 색소 침착 질환을 예방 또는 개선할 수 있는 건강기능식품 또는 치료제로서 유용하게 사용할 수 있다.The present invention relates to a plasma-treated red pepper leaf extract and a method for producing the same, the skin whitening effect is enhanced, the total polyphenol content is increased compared to the red pepper leaf extract not treated with plasma, antioxidant effect and melanin production Since the effect of inhibiting the remarkably enhanced, the composition containing the red pepper leaf extract prepared by the manufacturing method of the present invention as an active ingredient, functional cosmetics for skin whitening, health functional food or therapeutic agent that can prevent or improve skin pigmentation disease It can be usefully used as.
Description
본 발명은 피부 미백효과가 증진된 플라즈마 처리된 고춧잎 추출물 및 그의 제조방법에 관한 것이다. The present invention relates to a plasma-treated pepper leaf extract with improved skin lightening effect and a method for producing the same.
자외선에 피부가 노출되면 사람의 피부색을 결정하는 멜라닌(melanin)이 합성, 방출되어 기미, 주근깨, 점 등과 같은 과색소 침착증을 유발하여 미용상 좋지 않은 결과를 가져오게 되며, 과도하게 생성된 멜라닌 색소를 재빨리 방출해서 그 사람이 지니고 있는 본래 피부의 투명함을 되찾는 일을 피부 미백이라고 한다. When the skin is exposed to ultraviolet rays, melanin (melanin), which determines the color of a person's skin, is synthesized and released, causing hyperpigmentation such as blemishes, freckles, and spots, resulting in cosmetically bad results. It is called skin whitening that releases quickly and restores the transparency of the person's original skin.
멜라닌은 세포내의 티로신(tyrosine)을 기질로 하여 티로시나아제(tyrosinase)라는 효소가 도파퀴논(dopaquinone)을 생성시키고, 도파퀴논으로부터 자동산화 반응과 효소반응을 거쳐 합성된다. 따라서 멜라닌 생성과정 중 일부반응을 저해하여 멜라닌 합성 자체를 감소시키거나, 멜라닌이 형성된 세포를 파괴하는 방법으로 피부 미백효과를 나타낼 수 있다. Melanin is the substrate of tyrosine in the cell (tyrosinase) enzyme tyrosinase (tyrosinase) to produce dopaquinone (dopaquinone) is synthesized from the dopaquinone through the automatic oxidation and enzyme reaction. Therefore, by inhibiting some reactions during the production of melanin, the melanin synthesis itself may be reduced or melanin may form a skin whitening effect by destroying cells.
식품의약품안전청에 미백 성분으로 등록된 물질은 닥나무 추출물, 알부틴, 에틸아스코빌에텔, 유용성 감초 추출물, 아스코빌글루코사이드, 나이아신아마이드, 알파-비사볼올, 아스코빌테트라이소팔미테이트 등 모두 8가지이다. 이 물질을 이용해 로션이나 액제, 크림, 마스크팩 등을 만드는 것을 미백화장품이라 한다.There are eight substances registered with the Korea Food and Drug Administration as whitening ingredients, such as mulberry extract, arbutin, ethyl ascorbyl ether, oil-soluble licorice extract, ascorbyl glucoside, niacinamide, alpha-bisabolol, and ascorbyl triisopalmitate. Using this substance to make lotions, liquids, creams and mask packs is called whitening cosmetics.
닥나무 추출물과 알부틴, 알파-비사볼올, 유용성 감초 추출물은 멜라닌을 만드는데 관련된 효소인 티로시나아제가 활성화되는 것을 막는다. 아스코빌글루코사이드와 에틸아스코빌에텔, 아스코빌테트라이소팔미테이트는 티로시나아제 효소에 자극받은 티로신이 산화되는 것을 막아준다. 나이아신아마이드는 이미 생성된 멜라닌이 멜라노사이트에서 각질형성세포로 넘어가는 단계를 억제하여 멜라닌이 실제 피부 세포에 들어가는 마지막 단계를 막는다.Its extracts, arbutin, alpha-bisabolol and oil-soluble licorice extracts prevent the activation of tyrosinase, an enzyme involved in making melanin. Ascorbylglucoside, ethyl ascorbyl ether and ascorbyl tetraisopalmitate prevent the oxidation of tyrosine stimulated by tyrosinase enzymes. Niacinamide inhibits the production of melanin from the melanocytes to keratinocytes, preventing the last stage of melanin from entering the skin cells.
미용이 아닌 의료 목적으로 사용되는 물질의 대표는 의약품으로 처방되는 하이드로퀴논이다. 하이드로퀴논은 주로 피부 연고로 사용된다. 한국에서 이 물질이 4% 이상 함유된 연고를 구입하려면 반드시 의사의 처방이 있어야 하며, 2% 연고는 처방전 없이도 약국에서 구입할 수 있다. 의료 목적으로 사용되는 하이드로퀴논 연고는 미백화장품과는 다르게 멜라닌이 있는 피부 세포를 직접 파괴해 새로운 피부 세포가 자라도록 돕는다.Representatives of substances used for non-cosmetic medical purposes are hydroquinones prescribed as pharmaceuticals. Hydroquinone is mainly used as a skin ointment. In Korea, ointments containing more than 4% of this substance must be prescribed by a doctor, and 2% can be purchased at a pharmacy without a prescription. Unlike whitening cosmetics, hydroquinone ointment, which is used for medical purposes, directly destroys skin cells with melanin and helps new skin cells grow.
하지만, 상기 물질들은 비교적 높은 농도에서만 티로시나아제 활성 저해 효과가 나타나거나, 안정성이 낮아 효과가 오래 지속되지 못하는 등 제품의 적용 범위가 좁다는 문제점이 있으며, 하이드로퀴논은 피부자극 및 안정성 문제로 인하여 화장료에서의 사용이 제한되고 있다.However, these substances have a problem that the application range of the product is narrow, such as the effect of inhibiting tyrosinase activity only at a relatively high concentration, or the stability does not last long, hydroquinone is due to skin irritation and stability problems Use in cosmetics is limited.
한편, 고춧잎은 수분 79.4%, 단백질 4.1%, 지질 1%, 섬유소 3.8%, 당질 8.2%, 회분 3.5%로 구성되어 있고, 풍부한 비타민 A, 비타민 C, 폴리페놀, 플라보노이드 등을 함유하고 있으며, DPPH 라디칼 소거능, 과산화수소 소거능 및 높은 항산화능을 가지고 있다는 것이 보고되었다. 또한, 유방암, 대장암 및 위암 등의 암세포 증식 억제효과, 보체계 활성효과 등 항암효과와 면역증진, 항균활성 및 티로시나아제(tyrosinase) 저해 활성 등을 가진다고 알려져 있다. 이러한 고춧잎의 어린순은 일부 식용으로 활용되고 있지만, 대부분의 고춧잎과 줄기 부위는 폐기되고 있다. 고춧잎은 고추 과육과 달리 매운맛이 없으면서 유용한 물질을 함유하고 있으므로 이를 활용한 기능성 소재의 개발시 고기능성의 고부가 제품을 개발할 수 있어 산업적 활용가치가 매우 높은 자원이다.On the other hand, red pepper leaves are composed of 79.4% moisture, 4.1% protein, 1% lipid, 3.8% fibrin, 8.2% sugar, and 3.5% ash, and contain abundant vitamin A, vitamin C, polyphenols, flavonoids, and DPPH. It has been reported to have radical scavenging activity, hydrogen peroxide scavenging activity and high antioxidant activity. In addition, it is known to have anticancer effects such as cancer cell proliferation inhibitory effect, complement system activation effect such as breast cancer, colorectal cancer and gastric cancer, immune enhancement, antibacterial activity and tyrosinase inhibitory activity. The young shoots of these pepper leaves are used for some food, but most of the pepper leaves and stems are discarded. Red pepper leaves, unlike red pepper flesh, do not have a spicy taste and contain useful substances, so they can develop high-value, high value-added products when developing functional materials using them.
한편, 전리된 가스 상태인 플라즈마에는 전자, 양이온, 음이온, 자유라디칼 및 광자 등을 포함하는 활성산소종이 존재한다. 그 중 열 플라즈마는 주로 아크 방전에 의해 발생시킨 전자, 이온, 중성입자로 구성된 기체로 구성입자가 1000~2000m/s를 갖는 고속의 제트 불꽃 형태를 이루고 있다. 이러한 고온, 고열용량, 고속, 다량의 활성입자를 갖는 열플라즈마의 특성을 이용하여 재래식 기술에서는 만들 수 없는 다양하고 효율적인 고온 열원이나 물리화학 반응자(reactor)로 사용되어, 여러 산업분야에서 이용되고 있다. On the other hand, active oxygen species including electrons, cations, anions, free radicals, photons, and the like exist in the ionized gas plasma. Among them, the thermal plasma is a gas composed mainly of electrons, ions, and neutral particles generated by arc discharge, and forms a high-speed jet flame having a constituent particle of 1000 to 2000 m / s. By using the characteristics of thermal plasma having high temperature, high heat capacity, high speed, and a large amount of active particles, it is used as various and efficient high temperature heat sources or physicochemical reactors which cannot be produced by conventional technology, and is used in various industrial fields. .
따라서 본 발명은 플라즈마 처리를 통해 우수한 효능소재인 고춧잎 추출물의 활성물질에 의한 효과를 더욱 증진시키고자 하였다. Therefore, the present invention intends to further enhance the effect of the active material of the pepper leaf extract which is an excellent efficacy material through plasma treatment.
한편, 한국등록특허 제1194226호에는 고추의 변종에 속하는 파프리카 효모발효 추출액을 포함하는 항산화 및 미백 화장료 조성물 및 그 제조방법이 개시되어 있고, 한국공개특허 제2017-0017142호에는 항산화활성 및 미백효과가 있는 기능성 고추장 및 그 제조방법이 개시되어 있으나, 본 발명의 피부 미백효과가 증진된 플라즈마 처리된 고춧잎 추출물 및 그의 제조방법에 대해 개시된 바 없다. Meanwhile, Korean Patent No. 1194226 discloses an antioxidant and whitening cosmetic composition including a paprika yeast fermentation extract belonging to a variety of red pepper and a method for preparing the same, and Korean Patent Application Publication No. 2017-0017142 has an antioxidant activity and a whitening effect. Although functional kochujang and a method for producing the same have been disclosed, the plasma-treated pepper leaf extract with enhanced skin whitening effect of the present invention and a method for producing the same have not been disclosed.
본 발명은 상기와 같은 요구에 의해 도출된 것으로, 피부 미백효과가 증진된 플라즈마 처리된 고춧잎 추출물의 제조방법을 제공하고, 상기 제조방법으로 제조된 플라즈마 처리된 고춧잎 추출물은 총 폴리페놀 함량이 증가하고, 항산화 및 피부 미백효과가 증진되는 것을 확인함으로써, 본 발명을 완성하였다. The present invention is derived from the above requirements, and provides a method for producing a plasma-treated pepper leaf extract with enhanced skin whitening effect, the plasma-treated pepper leaf extract prepared by the manufacturing method increases the total polyphenol content By confirming that the antioxidant and skin whitening effects are enhanced, the present invention has been completed.
상기 과제를 해결하기 위하여, 본 발명은 In order to solve the above problems, the present invention
1) 고춧잎 건조 분말에 용매를 첨가하여 고춧잎 추출물을 획득한 후 동결건조하는 단계; 및1) adding a solvent to the pepper leaf dry powder to obtain a pepper leaf extract and then lyophilized; And
2) 상기 단계 1)의 동결건조된 고춧잎 추출물 1 중량부에 대하여, 80~120 부피부의 물을 첨가한 후 플라즈마를 처리하는 단계;를 포함하는 피부 미백효과가 증진된 고춧잎 추출물의 제조방법을 제공한다. 2) to about 1 part by weight of the lyophilized red pepper leaf extract of step 1), adding 80 to 120 parts by volume of water and then treating the plasma; to provide.
또한, 본 발명은 상기 제조방법에 따라 제조된 피부 미백효과가 증진된 고춧잎 추출물을 제공한다. In addition, the present invention provides a red pepper leaf extract with enhanced skin whitening effect prepared according to the production method.
또한, 본 발명은 상기 고춧잎 추출물을 유효성분으로 함유하는 피부 미백용 화장료 조성물을 제공한다.The present invention also provides a cosmetic composition for skin whitening containing the pepper leaf extract as an active ingredient.
또한, 본 발명은 상기 고춧잎 추출물을 유효성분으로 함유하는 멜라닌 색소 과다 침착 질환의 예방 또는 개선용 건강기능식품 조성물을 제공한다.The present invention also provides a health functional food composition for the prevention or improvement of melanin pigment hyperdeposition disease containing the pepper leaf extract as an active ingredient.
또한, 본 발명은 상기 고춧잎 추출물을 유효성분으로 함유하는 멜라닌 색소 과다 침착 질환의 예방 또는 치료용 약학 조성물을 제공한다. In another aspect, the present invention provides a pharmaceutical composition for the prevention or treatment of melanin pigment hyperdeposition disease containing the pepper leaf extract as an active ingredient.
본 발명은 피부 미백효과가 증진된 플라즈마 처리된 고춧잎 추출물 및 그의 제조방법에 관한 것으로, 본 발명의 제조방법으로 제조된 플라즈마 처리된 고춧잎 추출이 일반 고춧잎 추출물에 비해 총 폴리페놀 함량이 증가하고, DPPH 및 ABTS 라디칼 소거능이 우수하며, 멜라닌 생성 억제효과 또한 우수하였다. The present invention relates to a plasma-treated red pepper leaf extract with improved skin whitening effect and a method for producing the same, wherein the plasma-treated red pepper leaf extract prepared by the method of the present invention has an increased total polyphenol content compared to general red pepper leaf extract, and DPPH And ABTS radical scavenging activity was excellent, melanin production inhibitory effect was also excellent.
도 1은 본 발명의 플라즈마 발생장치의 사진이다.
도 2는 본 발명의 제조방법으로 제조된 플라즈마 처리된 고춧잎 추출물의 세포독성을 확인한 결과이다. CON은 고춧잎 추출물 무처리 음성대조군이다.
도 3은 본 발명의 제조방법으로 제조된 플라즈마 처리된 고춧잎 추출물의 멜라닌 생합성 억제 정도를 나타낸 결과이다. α-MSH를 처리하여 멜라닌 생합성을 유도하였고, Ar은 알부틴(arbutin)으로 양성 대조군이며, CON은 α-MSH 및 고춧잎 추출물을 처리하지 않은 음성 대조군이다. a~f는 서로 유의한 차가 있음을 나타내며, p<0.05임을 의미한다. 1 is a photograph of a plasma generating apparatus of the present invention.
Figure 2 is a result confirming the cytotoxicity of the plasma-treated pepper leaf extract prepared by the manufacturing method of the present invention. CON is an untreated negative control of pepper leaf extract.
3 is a result showing the degree of melanin biosynthesis inhibition of the plasma-treated pepper leaf extract prepared by the manufacturing method of the present invention. Treatment with α-MSH induced melanin biosynthesis, Ar was a positive control with arbutin and CON was a negative control without α-MSH and pepper leaf extract. a to f represent a significant difference from each other, and mean p <0.05.
본 발명은 The present invention
1) 고춧잎 건조 분말에 용매를 첨가하여 고춧잎 추출물을 획득한 후 동결건조하는 단계; 및1) adding a solvent to the pepper leaf dry powder to obtain a pepper leaf extract and then lyophilized; And
2) 상기 단계 1)의 동결건조된 고춧잎 추출물 1 중량부에 대하여, 80~120 부피부의 물을 첨가한 후 플라즈마를 처리하는 단계;를 포함하는 피부 미백효과가 증진된 고춧잎 추출물의 제조방법에 관한 것이다. 2) to about 1 part by weight of the lyophilized red pepper leaf extract of step 1), adding 80 to 120 parts by volume of water and then treating the plasma; It is about.
상기 단계 1)의 용매는 물, C1~C4의 저급 알코올 또는 이들의 혼합물인 것이 바람직하고, 더욱 바람직하게는 에탄올을 용매로 이용하는 것이지만, 이에 제한하지 않는다. The solvent of step 1) is preferably water, lower alcohols of C 1 ~ C 4 or mixtures thereof, more preferably ethanol as a solvent, but is not limited thereto.
상기 단계 2)의 플라즈마 처리는 텅스턴 전극 사이에 사인파 펄스형 고전압을 가하여 생성된 플라즈마를 고춧잎 추출물에 처리하는 것이지만, 이에 제한되는 것은 아니다. Plasma treatment of step 2) is to process the plasma generated by applying a sinusoidal pulsed high voltage between the tungsten electrode to the pepper leaf extract, but is not limited thereto.
상기 고전압은 출력전압이 250~350V이고, 전류는 4~6A인 것이지만, 이에 한정하지 않는다. The high voltage has an output voltage of 250 to 350 V and a current of 4 to 6 A, but is not limited thereto.
본 발명의 플라즈마는 두 전극 사이에 전압을 걸어 전기장을 형성시킴으로써 전자파의 방전을 통해 발생시키는 저온 플라즈마이고, 대기압에서 플라즈마를 발생시키는 대기압 플라즈마이다. The plasma of the present invention is a low-temperature plasma generated through the discharge of electromagnetic waves by applying an electric voltage between two electrodes to form an electric field, and an atmospheric pressure plasma that generates plasma at atmospheric pressure.
본 발명의 바람직한 일 구현 예에서, In one preferred embodiment of the invention,
1) 고춧잎 건조 분말에 50~100%(v/v) 에탄올을 첨가하여 15~30℃의 온도에서 5~15시간 동안 추출하여 고춧잎 추출물을 획득한 후 여과하고, 여과된 여액을 동결건조하는 단계; 및1) adding 50 ~ 100% (v / v) ethanol to the pepper leaf dry powder for 5 to 15 hours to extract at a temperature of 15 ~ 30 ℃ to obtain a pepper leaf extract and filtered, and the filtered filtrate lyophilized ; And
2) 상기 단계 1)의 동결건조된 고춧잎 추출물 1g에 대하여 80~120㎖의 물을 첨가한 후 텅스턴 전극 사이에 사인파 펄스형 고전압을 가하여 생성된 플라즈마를 처리하는 단계;를 포함하여 피부 미백효과가 증진된 고춧잎 추출물을 제조할 수 있으나, 이에 한정하는 것은 아니다.2) adding 80-120 ml of water to 1 g of the lyophilized red pepper leaf extract of step 1) and then applying a sinusoidal pulsed high voltage between the tungsten electrodes to process the generated plasma. Can be prepared to enhance the pepper leaf extract, but is not limited thereto.
또한, 본 발명은 상기 제조방법에 따라 제조된 피부 미백효과가 증진된 고춧잎 추출물에 관한 것이다. In addition, the present invention relates to a pepper leaf extract with enhanced skin whitening effect prepared according to the production method.
상기 고춧잎 추출물은 총 폴리페놀 함량이 일반 고춧잎 추출물에 비해 증가하고, 항산화 활성이 증진된다. The pepper leaf extract has a total polyphenol content is increased compared to the general pepper leaf extract, antioxidant activity is enhanced.
또한, 본 발명은 상기 고춧잎 추출물을 유효성분으로 함유하는 피부 미백용 화장료 조성물에 관한 것이다. The present invention also relates to a cosmetic composition for skin whitening containing the pepper leaf extract as an active ingredient.
본 발명의 일 구현 예에 따른 화장료 조성물에 있어서, 상기 화장료 조성물은 멜라닌 생합성을 억제할 수 있지만, 이에 제한되지 않는다. In the cosmetic composition according to an embodiment of the present invention, the cosmetic composition may inhibit melanin biosynthesis, but is not limited thereto.
본 발명에서 피부 미백효과란, 자외선 노출, 호르몬 밸런스의 변화, 유전적 프로그램 등의 각종 요인에 의해 발생하는 거뭇한 피부나 기미, 주근깨, 다크서클을 개선 또는 방지하는 효과, 피부를 투명감 있는 아름다운 것으로 하는, 혹은 투명감 있는 아름다운 피부를 유지하는 효과, 피부의 칙칙함을 경감하여 윤기ㆍ팽팽함을 증가시키는 효과 등을 의도하고 있다. 일반적으로, 거뭇한 피부나 기미, 주근깨, 다크서클은 자외선에 의한 자극이나 호르몬 밸런스의 변화 등에 의해 멜라노사이트(melanocyte)가 자극되고, 이로 인해 생합성된 멜라닌 색소가 피부에 침착하여 발생한다고 알려져 있다. 따라서, 멜라닌의 생성을 억제할 수 있다면, 거뭇한 피부나 기미, 주근깨, 다크서클을 예방ㆍ개선하는 것이 가능하다.In the present invention, the skin whitening effect is an effect of improving or preventing dark skin, blemishes, freckles, and dark circles caused by various factors such as UV exposure, hormonal balance change, and genetic program, and beautiful skin with transparency. It is intended to maintain the beautiful skin with a sense of transparency, or to reduce the dullness of the skin and to increase the luster and tension. In general, dark skin, blemishes, freckles, and dark circles are known to stimulate melanocytes (melanocytes) due to stimulation caused by ultraviolet rays or changes in hormonal balance, resulting in the deposition of biosynthetic melanin pigments on the skin. Therefore, if the production of melanin can be suppressed, it is possible to prevent and improve dark skin, blemishes, freckles and dark circles.
본 발명의 일 구현 예에 따른 화장료 조성물에 있어서, 상기 피부 미백용 화장료 조성물은 스킨, 스킨 소프트너, 스킨 토너, 로션, 밀크로션, 모이스쳐로션, 영양로션, 마사지크림, 영양크림, 아이크림, 모이스쳐크림, 핸드크림, 에센스, 영양에센스, 팩, 클렌징폼, 클렌징워터, 클렌징크림, 바디로션, 바디클렌져, 비누 및 파우더 중에서 선택된 어느 하나의 제형을 가질 수 있으나, 이에 제한되지 않는다. 이들 각 제형으로 이루어진 화장료 조성물은 그 제형의 제제화에 필요하고 적절한 각종의 기제와 첨가물을 함유할 수 있으며, 이들 성분의 종류와 양은 당업자에 의해 용이하게 선정될 수 있다. In the cosmetic composition according to an embodiment of the present invention, the skin whitening cosmetic composition is a skin, skin softener, skin toner, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, eye cream, moisturizer Cream, hand cream, essence, nutrition essence, pack, cleansing foam, cleansing water, cleansing cream, body lotion, body cleanser, soap and powder may have any one selected from, but is not limited thereto. The cosmetic composition consisting of each of these formulations may contain various bases and additives necessary for the formulation of the formulation and are suitable, and the type and amount of these components can be easily selected by those skilled in the art.
본 발명의 화장료 조성물은 유효성분 이외에 추가로 동일 또는 유사한 기능을 나타내는 피부 미백 활성 성분을 1종 이상 함유할 수 있다. 피부 미백 활성 성분으로는 코지산 및 이의 유도체, 알부틴, 아스코르브산 및 이의 유도체, 하이드로퀴논 및 이의 유도체, 레조르시놀, 사이클로알카논, 메틸렌디옥시페닐 알칸올, 2,7-디니트로인다졸 또는 덩굴귤 추출물, 쌀 추출물, 감초 추출물과 같은 식물 추출물 등이 있으나, 이에 제한되는 것은 아니다.The cosmetic composition of the present invention may further contain one or more skin whitening active ingredients exhibiting the same or similar functions in addition to the active ingredient. Skin whitening active ingredients include kojic acid and derivatives thereof, arbutin, ascorbic acid and derivatives thereof, hydroquinone and derivatives thereof, resorcinol, cycloalkanone, methylenedioxyphenyl alkanol, 2,7-dinitroindazole or Plant extracts such as vine tangerine extract, rice extract, licorice extract, and the like, but is not limited thereto.
본 발명의 화장료 조성물의 제형이 페이스트, 크림 또는 겔인 경우에는 담체 성분으로서 동물섬유, 식물섬유, 왁스, 파라핀, 전분, 트라가칸트, 셀룰로오스 유도체, 폴리에틸렌 글리콜, 실리콘, 벤토나이트, 실리카, 탈크 또는 산화아연 등이 이용될 수 있다.When the formulation of the cosmetic composition of the present invention is a paste, cream or gel, the carrier component is animal fiber, vegetable fiber, wax, paraffin, starch, tragacanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide. And the like can be used.
본 발명의 화장료 조성물의 제형이 파우더 또는 스프레이인 경우에는 담체 성분으로서 락토스, 탈크, 실리카, 알루미늄 히드록시드, 칼슘 실리케이트 또는 폴리아미드 파우더가 이용될 수 있고, 특히 스프레이인 경우에는 추가적으로 클로로플루오로히드로카본, 프로판-부탄 또는 디메틸 에테르와 같은 추진체를 포함할 수 있다.When the formulation of the cosmetic composition of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as the carrier component, and in particular, in the case of a spray, additionally chlorofluorohydro Propellants such as carbon, propane-butane or dimethyl ether.
본 발명의 화장료 조성물의 제형이 용액 또는 유탁액인 경우에는 담체 성분으로서 용매, 용매화제 또는 유탁화제가 이용되고, 예컨대 물, 에탄올, 이소프로판올, 에틸 카보네이트, 에틸 아세테이트, 벤질 알코올, 벤질 벤조에이트, 프로필렌글리콜, 1,3-부틸글리콜 오일, 글리세롤 지방족 에스테르, 폴리에틸렌 글리콜 또는 소르비탄의 지방산 에스테르가 있다.When the formulation of the cosmetic composition of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene. Fatty acid esters of glycol, 1,3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.
본 발명의 화장료 조성물의 제형이 현탁액인 경우에는 담체 성분으로서 물, 에탄올 또는 프로필렌 글리콜과 같은 액상 희석제, 에톡실화 이소스테아릴 알코올, 폴리옥시에틸렌 소르비톨 에스테르 및 폴리옥시에틸렌 소르비탄 에스테르와 같은 현탁제, 미소결정성 셀룰로오스, 알루미늄 메타히드록시드, 벤토나이트, 아가 또는 트라칸트 등이 이용될 수 있다.When the formulation of the cosmetic composition of the present invention is a suspension, as a carrier component, water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Microcrystalline cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.
본 발명의 화장료 조성물의 제형이 계면-활성제 함유 클렌징인 경우에는 담체 성분으로서 지방족 알코올 설페이트, 지방족 알코올 에테르설페이트, 설포숙신산 모노에스테르, 아세티오네이트, 이미다졸리늄 유도체, 메틸타우레이트, 사르코시네이트, 지방산 아미드 에테르 설페이트, 알킬아미도베타인, 지방족 알코올, 지방산 글리세리드, 지방산 디에탄올아미드, 식물성 유, 리놀린 유도체 또는 에톡실화 글리세롤 지방산 에스테르 등이 이용될 수 있다.When the formulation of the cosmetic composition of the present invention is a surfactant-containing cleansing agent, as a carrier component, an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, acethionate, an imidazolinium derivative, methyl taurate, and sarcosinate Fatty acid amide ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.
본 발명의 화장료 조성물은 형광물질, 살진균제, 굴수성 유발물질, 보습제, 방향제, 방향제 담체, 단백질, 용해화제, 당 유도체, 일광차단제, 비타민, 식물 추출물 등을 포함하는 부형제를 추가로 함유할 수 있다.The cosmetic composition of the present invention may further contain an excipient including a fluorescent substance, a fungicide, a caustic agent, a moisturizer, a fragrance, a fragrance carrier, a protein, a solubilizer, a sugar derivative, a sunscreen agent, a vitamin, a plant extract, and the like. .
또한, 본 발명은 상기 고춧잎 추출물을 유효성분으로 함유하는 멜라닌 색소 과다 침착 질환의 예방 또는 개선용 건강기능식품 조성물에 관한 것이다. The present invention also relates to a nutraceutical composition for the prevention or improvement of melanin hyperpigmentation disease containing the red pepper leaf extract as an active ingredient.
본 명세서에서 사용되는 용어 "멜라닌 색소 과다 침착(hyperpigmentation)"은 피부 또는 손발톱의 특정 부위에서 멜라닌의 과도한 증가에 의해 다른 부위에 비해 검게 또는 어둡게 되는 것을 의미한다. 상기 멜라닌 색소 과다 침착 질환은 주근깨, 노인성 반점, 간반, 기미, 갈색 또는 흑점, 일광 색소반, 푸른흑피증(cyanic melasma), 약물 사용 후의 과다 색소 침착; 찰상, 화상 또는 피부염으로 인한 염증 후 과다 색소 침착; 또는 임신성 갈색반(gravidic chloasma) 등을 포함하나 이에 한정되지 않는다.As used herein, the term "melanin hyperpigmentation" means that the skin or nails become black or dark compared to other areas by excessive increase of melanin in certain areas. The melanin hyperpigmentation diseases include freckles, senile plaques, liver spots, blemishes, brown or sunspots, sunrays, cyanic melasma, hyperpigmentation after drug use; Hyperpigmentation after inflammation due to scratches, burns or dermatitis; Or gravureic chloasma, and the like.
본 발명의 건강기능식품 조성물을 식품첨가물로 사용하는 경우, 상기 건강기능식품 조성물을 그대로 첨가하거나 다른 식품 또는 식품성분과 함께 사용될 수 있고, 통상적인 방법에 따라 적절하게 사용될 수 있다. 유효성분의 양은 그의 사용 목적(예방 또는 개선)에 따라 적절하게 사용될 수 있다. 일반적으로, 식품 또는 음료의 제조시 본 발명의 건강기능식품 조성물은 원료에 대하여 15 중량부 이하, 바람직하게는 10 중량부 이하의 양으로 첨가된다. 그러나 건강을 목적으로 하는 장기간의 섭취인 경우에는 상기 양은 상기 범위 이하일 수 있으며, 안전성 면에서 아무런 문제가 없기 때문에 유효성분은 상기 범위 이상의 양으로 사용될 수 있다.When the health functional food composition of the present invention is used as a food additive, the health functional food composition may be added as it is or used with other foods or food ingredients, and may be appropriately used according to a conventional method. The amount of the active ingredient may be appropriately used depending on the purpose of use (prevention or improvement). In general, in the preparation of food or beverages, the nutraceutical composition of the present invention is added in an amount of 15 parts by weight or less, preferably 10 parts by weight or less with respect to the raw material. However, in the case of long-term intake for health purposes, the amount may be below the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount above the above range.
상기 건강기능식품의 종류에 특별한 제한은 없다. 상기 건강기능식품 조성물을 첨가할 수 있는 식품의 예로는 육류, 소시지, 빵, 초콜릿, 캔디류, 스낵류, 과자류, 피자, 라면, 기타 면류, 껌류, 아이스크림류를 포함한 낙농제품, 각종 스프, 음료수, 차 드링크제, 알콜 음료 및 비타민 복합제 등이 있으며, 통상적인 의미에서의 건강식품을 모두 포함한다.There is no particular limitation on the type of dietary supplement. Examples of foods to which the health functional food composition may be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gums, dairy products, including ice cream, various soups, drinks, tea Drinks, alcoholic beverages, vitamin complexes, and the like, and include all health foods in the conventional sense.
또한, 본 발명의 건강기능식품 조성물은 식품, 특히 기능성 식품으로 제조될 수 있다. 본 발명의 기능성 식품은 식품 제조 시에 통상적으로 첨가되는 성분을 포함하며, 예를 들어, 단백질, 탄수화물, 지방, 영양소 및 조미제를 포함한다. 예컨대, 드링크제로 제조되는 경우에는 유효성분 이외에 천연 탄수화물 또는 향미제를 추가 성분으로서 포함할 수 있다. 상기 천연 탄수화물은 모노사카라이드(예컨대, 글루코오스, 프럭토오스 등), 디사카라이드(예컨대, 말토스, 수크로오스 등), 올리고당, 폴리사카라이드(예컨대, 덱스트린, 시클로덱스트린 등) 또는 당알코올(예컨대, 자일리톨, 소르비톨, 에리쓰리톨 등)인 것이 바람직하다. 상기 향미제는 천연 향미제(예컨대, 타우마틴, 스테비아 추출물 등)와 합성 향미제(예컨대, 사카린, 아스파르탐 등)를 이용할 수 있다.In addition, the nutraceutical composition of the present invention may be prepared as a food, in particular a functional food. Functional foods of the present invention include ingredients that are commonly added in food production, and include, for example, proteins, carbohydrates, fats, nutrients and seasonings. For example, when prepared with a drink, natural carbohydrates or flavoring agents may be included as additional ingredients in addition to the active ingredient. The natural carbohydrates can be monosaccharides (e.g. glucose, fructose, etc.), disaccharides (e.g. maltose, sucrose, etc.), oligosaccharides, polysaccharides (e.g. dextrins, cyclodextrins, etc.) or sugar alcohols (e.g. , Xylitol, sorbitol, erythritol and the like). The flavourant may be a natural flavourant (eg, taumartin, stevia extract, etc.) and a synthetic flavourant (eg, saccharin, aspartame, etc.).
상기 건강기능식품 조성물 이외에 여러 가지 영양제, 비타민, 전해질, 풍미제, 착색제, 펙트산 및 그의 염, 알긴산 및 그의 염, 유기산, 보호성 콜로이드 증점제, pH 조절제, 안정화제, 방부제, 글리세린, 알코올, 탄산음료에 사용되는 탄산화제 등을 더 함유할 수 있다. 이러한 상기 첨가되는 성분의 비율은 크게 중요하진 않지만 본 발명의 건강기능식품 조성물 100 중량부에 대하여, 0.01 내지 0.1 중량부의 범위에서 선택되는 것이 일반적이다.Various nutritional supplements, vitamins, electrolytes, flavors, coloring agents, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH regulators, stabilizers, preservatives, glycerin, alcohols, carbonic acid The carbonation agent etc. which are used for a drink can be contained further. Although the ratio of the above-mentioned ingredients is not critical, it is generally selected from 0.01 to 0.1 parts by weight based on 100 parts by weight of the health functional food composition of the present invention.
또한, 본 발명은 상기 고춧잎 추출물을 유효성분으로 함유하는 멜라닌 색소 과다 침착 질환의 예방 또는 치료용 약학 조성물에 관한 것이다. In addition, the present invention relates to a pharmaceutical composition for the prevention or treatment of melanin-pigmentation hyperplastic disorders containing the red pepper leaf extract as an active ingredient.
본 발명의 약학조성물은 유효성분 이외에 약학적으로 허용되는 담체를 포함할 수 있으며, 이러한 담체는 제제 시에 통상적으로 이용되는 것으로서, 락토스, 덱스트로스, 수크로스, 솔비톨, 만니톨, 전분, 아카시아 고무, 인산 칼슘, 알기네이트, 젤라틴, 규산 칼슘, 미세결정성 셀룰로스, 폴리비닐피롤리돈, 셀룰로스, 물, 시럽, 메틸 셀룰로스, 메틸히드록시벤조에이트, 프로필히드록시벤조에이트, 활석, 스테아르산 마그네슘 및 미네랄 오일 등을 포함하나, 이에 한정되는 것은 아니다. 본 발명의 약학적 조성물은 상기 성분들 이외에 윤활제, 습윤제, 감미제, 향미제, 유화제, 현탁제, 보존제 등을 추가로 포함할 수 있다. The pharmaceutical composition of the present invention may include a pharmaceutically acceptable carrier in addition to the active ingredient, and such carriers are commonly used in the preparation of lactose, dextrose, sucrose, sorbitol, mannitol, starch, acacia rubber, Calcium phosphate, alginate, gelatin, calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, water, syrup, methyl cellulose, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and minerals Oils and the like, but are not limited thereto. In addition to the above components, the pharmaceutical composition of the present invention may further include a lubricant, a humectant, a sweetener, a flavoring agent, an emulsifier, a suspending agent, a preservative, and the like.
본 발명에 따른 약학조성물의 적합한 투여량은 제제화 방법, 투여 방식, 환자의 연령, 체중, 성, 병적 상태, 음식, 투여 시간, 투여 경로, 배설 속도 및 반응 감응성과 같은 요인들에 의해 다양하게 처방될 수 있다. Suitable dosages of the pharmaceutical compositions according to the present invention may be variously prescribed by such factors as the formulation method, mode of administration, age, weight, sex, morbidity, food, time of administration, route of administration, rate of excretion and response to the patient. Can be.
본 발명의 약학조성물은 경구 또는 비경구로 투여할 수 있으며, 비경구 투여의 경우, 피부에 국소적으로 도포, 정맥 내 주입, 피하 주입, 근육 주입, 복강 주입, 경피 투여 등으로 투여할 수 있다. 본 발명의 약학조성물이 멜라닌 색소 과다 침착 질환의 치료 또는 예방을 위해 적용되는 점을 감안하면, 피부에 국소적으로 도포되어 이루어지는 것이 바람직하다.The pharmaceutical composition of the present invention can be administered orally or parenterally, and in the case of parenteral administration, it can be administered topically to the skin, intravenous injection, subcutaneous injection, intramuscular injection, intraperitoneal injection, transdermal administration and the like. In consideration of the fact that the pharmaceutical composition of the present invention is applied for the treatment or prevention of melanin hyperpigmentation disease, it is preferable that the pharmaceutical composition is applied to the skin topically.
본 발명의 조성물에 포함되는 유효성분의 농도는 치료 목적, 환자의 상태, 필요기간 등을 고려하여 결정할 수 있으며, 특정 범위의 농도로 한정되지 않는다.The concentration of the active ingredient included in the composition of the present invention can be determined in consideration of the purpose of treatment, the condition of the patient, the period of time, etc., and is not limited to a specific range of concentration.
본 발명의 약학조성물은 당해 발명이 속하는 기술분야에서 통상의 지식을 가진 자가 용이하게 실시할 수 있는 방법에 따라, 약학적으로 허용되는 담체 또는 부형제를 이용하여 제제화함으로써 단위 용량 형태로 제조되거나 또는 다용량 용기 내에 내입시켜 제조될 수 있다. 이때 제형은 주사제, 크림, 패취, 분무제, 연고제, 경고제, 로션제, 리니멘트제, 파스타제 및 카타플라스마제 중에서 선택된 어느 하나의 제형으로 제조될 수도 있으며, 분산제 또는 안정화제를 추가적으로 포함할 수 있다.The pharmaceutical compositions of the present invention may be prepared in unit dosage form by formulating with a pharmaceutically acceptable carrier or excipient according to methods which can be easily carried out by those skilled in the art. It can be prepared by incorporation into a dose container. In this case, the formulation may be prepared in any one formulation selected from injections, creams, patches, sprays, ointments, warnings, lotions, linings, pastas, and cataplasmas, and may further include dispersants or stabilizers. have.
이하, 제조예 및 실시예를 이용하여 본 발명을 더욱 상세하게 설명하고자 한다. 이들 제조예 및 실시예는 오로지 본 발명을 보다 구체적으로 설명하기 위한 것으로 본 발명의 범위가 이들에 의해 제한되지 않는다는 것은 당해 기술분야에서 통상의 지식을 가진 자에게 있어 자명한 것이다. Hereinafter, the present invention will be described in more detail with reference to Preparation Examples and Examples. These preparations and examples are only intended to explain the present invention more specifically, it is apparent to those skilled in the art that the scope of the present invention is not limited thereto.
제조예Production Example 1. One. 플라즈마plasma 처리된 고춧잎 추출물의 제조 Preparation of Treated Pepper Leaf Extract
(1) 고춧잎 추출물의 제조(1) Preparation of Red Pepper Leaf Extract
본 발명의 고춧잎은 영양지역에서 재배된 PR우뚝 품종을 사용하였다. 건조하여 분쇄한 PR우뚝 품종의 고춧잎 3g에 70%(v/v) 에탄올을 30㎖ 넣고, 상온에서 12시간 동안 진탕하며 추출한 후, 필터하여 동결건조하였다. The pepper leaf of the present invention used the PR tower varieties cultivated in the trophic region. 30 g of 70% (v / v) ethanol was added to 3 g of dried red pepper pulverized varieties of pepper, extracted after shaking for 12 hours at room temperature, and filtered and lyophilized.
(2) 플라즈마 처리된 고춧잎 추출물의 제조(2) Preparation of Plasma Treated Red Pepper Leaf Extract
상기 동결건조한 고춧잎 에탄올 추출물 7g에 증류수 700㎖를 첨가하여 1%(w/v) 용액을 만든 후, 상기 용액을 플라즈마 발생기에 넣고, 0분, 30분, 60분, 90분 및 120분 동안 플라즈마 처리된 고춧잎 추출물을 제조하였다.After adding 700 ml of distilled water to 7 g of the lyophilized red pepper leaf ethanol extract to make a 1% (w / v) solution, the solution was placed in a plasma generator, and plasma was maintained for 0, 30, 60, 90 and 120 minutes. The treated pepper leaf extract was prepared.
본 발명에 사용한 플라즈마는 두 개의 텅스텐 전극 사이에 사인파 펄스형 고전압을 가하여 생성하였으며, 플라즈마 발생기는 도 1에 개시한 바와 같이 전압/고주파 전원장치, 플라즈마 방전부, 처리 테이블의 3 부분으로 구성된다. 플라즈마 방전부는 두께 5mm의 테프론 실드에 지름 3mm, 길이 20mm의 텅스텐 리드를 평행 배치한 후 매립하였다. 220V AC의 전원을 사용하였고, 출력전압은 300V, 전류는 5A로 고정하여 플라즈마를 생성하여 고춧잎 추출물에 처리하였다. The plasma used in the present invention was generated by applying a sinusoidal pulsed high voltage between two tungsten electrodes, and the plasma generator is composed of three parts: a voltage / high frequency power supply, a plasma discharge unit, and a treatment table. The plasma discharge part was embedded in a teflon shield having a thickness of 5 mm by placing a tungsten lead having a diameter of 3 mm and a length of 20 mm in parallel. 220V AC power was used, and the output voltage was fixed at 300V and the current was set at 5A to generate plasma and to process the pepper leaf extract.
실시예EXAMPLE 1. 총 폴리페놀 함량 및 1. Total polyphenol content and 항산화능Antioxidant activity 측정 Measure
본 발명의 제조방법으로 제조된 플라즈마 처리된 고춧잎 추출물의 총 폴리페놀 함량 및 항산화능을 측정하였다. Total polyphenol content and antioxidant activity of the plasma-treated pepper leaf extract prepared by the preparation method of the present invention were measured.
총 폴리페놀 함량은 표 1에 나타난 바와 같이 120분 동안 플라즈마를 처리하였을 때 증가하는 것을 확인하였고, ABTS 및 DPPH 라디칼 소거능 또한 120분 동안 플라즈마를 처리하였을 때 가장 증가하였다. 이를 통해 120분 동안 플라즈마를 처리한 고춧잎 추출물은 총 폴리페놀 함량 및 항산화능이 향상되는 것을 확인하였다. As shown in Table 1, the total polyphenol content was found to increase when the plasma was treated for 120 minutes, and the ABTS and DPPH radical scavenging ability also increased the most when the plasma was treated for 120 minutes. It was confirmed that the pepper leaf extract treated with plasma for 120 minutes improved the total polyphenol content and antioxidant activity.
(분)Plasma treatment
(minute)
(mg/추출물 1g)Total polyphenol content
(mg / extract 1g)
(conc. 1000㎍/㎖)Radical scavenging ability (%)
(conc. 1000 μg / ml)
실시예EXAMPLE 2. 세포생존율( 2. Cell survival rate ( cellcell viabilityviability ) 측정) Measure
제조예 1의 플라즈마 처리된 고춧잎 추출물이 세포생존에 미치는 영향을 확인하기 위하여 Mosmann(1983)의 방법을 이용하여 MTT(3-(4,5-dimeth-ylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) 분석을 실시하였다.In order to confirm the effect of the plasma-treated pepper leaf extract of Preparation Example 1 on the cell survival using the method of Mosmann (1983) MTT (3- (4,5-dimeth-ylthiazol-2-yl) -2,5- diphenyltetrazolium bromide) analysis was performed.
B16-F10 마우스 멜라노마 세포를 100㎕의 DMEM 배지가 담긴 96-웰 플레이트에 1일 동안 배양한 후, 제조예 1의 0~120분 동안 플라즈마가 처리된 고춧잎 추출물 100 또는 200㎍/㎖을 처리하여 24시간 배양하였다. 그 후, 각 웰에 5㎎/㎖의 MTT 용액을 10㎕씩 분주한 후 3시간 동안 배양하면서 환원반응을 유도하였으며, 100㎕의 DMSO(dimethyl sulfoxide) 용액을 첨가하여 보라색의 포르마잔(formazan) 결정을 완전히 용해하였다. 그 후, 분광광도계를 이용하여 570㎚에서 흡광도를 측정하였으며, 고춧잎 추출물 무처리군의 생존율 100%를 기준으로 고춧잎 추출물 처리군의 상대적 세포 생존율을 계산하였다. After culturing B16-F10 mouse melanoma cells in a 96-well plate containing 100 μl of DMEM medium for 1 day, 100 or 200 μg / ml of the pepper leaf extract treated with plasma for 0 to 120 minutes of Preparation Example 1 was treated. Incubated for 24 hours. Thereafter, 10 μl of 5 mg / ml MTT solution was dispensed into each well, followed by incubation for 3 hours to induce a reduction reaction, and 100 μl of DMSO (dimethyl sulfoxide) solution was added to form purple formazan (formazan). The crystals were completely dissolved. Thereafter, the absorbance was measured at 570 nm using a spectrophotometer, and the relative cell viability of the pepper leaf extract treatment group was calculated based on 100% survival of the pepper leaf extract untreated group.
그 결과, 도 2에 나타난 바와 같이 제조예 1의 0~120분 동안 플라즈마가 처리된 고춧잎 추출물은 200㎍/㎖의 농도까지 세포 생존율에 크게 영향을 주지 않은 것으로 보아 세포 독성이 거의 없다는 것을 알 수 있었다.As a result, as shown in Figure 2, the red pepper leaf extract treated with plasma for 0 to 120 minutes of Preparation Example 1 did not significantly affect cell viability up to a concentration of 200 µg / ml, indicating that there is little cytotoxicity. there was.
실시예EXAMPLE 3. 3. 플라즈마plasma 처리된 고춧잎 추출물의 멜라닌( Melanin from Red Pepper Leaves Extracts Treated melaninmelanin ) 생합성 억제효과Inhibitory effect on biosynthesis
B16-F10 마우스 멜라노마 세포를 12-웰 플레이트에 0.5×105 cells/㎖이 되도록 접종하여 24시간 동안 배양한 후, 각 웰에 200nM의 α-MSH(melanocyte-stimulating hormone)를 처리하여 멜라닌 생합성을 유도한 뒤 알부틴(arbutin, 100㎍/㎖) 또는 제조예 1의 0~120분 동안 플라즈마가 처리된 고춧잎 추출물을 각각 48시간 동안 처리하였다. 48시간 후 배지를 제거하고 인산완충액(phosphatate buffer saline, PBS)으로 2회 세척한 후 용해 완충액(0.1 M sodium phosphate buffer, 1% triton X-100, 0.2 mM PMSF)을 첨가한 후, 세포 스크레퍼를 이용하여 세포를 긁어 모은 다음 원심분리하여 세포 침전물을 획득하였다. 침전된 멜라노마 세포에 1N NaOH에 DMSO가 10% 함유되도록 만든 용액 200㎕를 가하고, 80℃에서 1시간 동안 용해하였으며, 405㎚에서 흡광도를 측정하였다. B16-F10 mouse melanoma cells were inoculated in a 12-well plate at 0.5 × 10 5 cells / ml and incubated for 24 hours, and then each well was treated with 200 nM of α-MSH (melanocyte-stimulating hormone) to synthesize melanin biosynthesis. After induction of arbutin (arbutin, 100㎍ / ㎖) or the pepper leaf extract treated with plasma for 0 to 120 minutes of Preparation Example 1 were treated for 48 hours each. After 48 hours, the medium was removed, washed twice with phosphatate buffer saline (PBS), and then lysis buffer (0.1 M sodium phosphate buffer, 1% triton X-100, 0.2 mM PMSF) was added. Cells were scraped and collected by centrifugation to obtain cell precipitates. To the precipitated melanoma cells, 200 μl of a solution made to contain 10% DMSO in 1N NaOH was added, dissolved at 80 ° C. for 1 hour, and absorbance was measured at 405 nm.
그 결과, 도 3에 나타난 바와 같이 α-MSH에 의해 증가된 멜라닌 함량은 200㎍/㎖의 시료를 처리한 경우 모든 시료 처리군에서 감소하였고, 플라즈마 처리시간에 비례하여 멜라닌 함량 감소효과가 우수하였으며, 플라즈마를 90분 이상 처리한 경우 가장 우수한 멜라닌 함량 감소효과를 나타냈다. As a result, as shown in FIG. 3, the melanin content increased by α-MSH was reduced in all sample treatment groups when the 200 μg / ml sample was treated, and the melanin content reduction effect was excellent in proportion to the plasma treatment time. When the plasma was treated for more than 90 minutes, it showed the best melanin content reduction effect.
100㎍/㎖의 시료를 처리한 경우 플라즈마를 처리하지 않은 고춧잎 추출물에서는 멜라닌 함량 감소효과가 나타나지 않았으나, 90분~120분 동안 플라즈마를 처리한 경우 멜라닌 함량 감소효과를 나타냈다. When the 100 ㎍ / ㎖ sample was treated with the plasma treatment did not show melanin content reduction effect in the pepper leaf extract, but the plasma treatment for 90 minutes to 120 minutes showed a melanin content reduction effect.
따라서 본 발명의 플라즈마 처리된 고춧잎 추출물은 피부 미백을 위한 기능성 소재로 사용 가능하다. Therefore, the plasma-treated pepper leaf extract of the present invention can be used as a functional material for skin whitening.
Claims (9)
2) 상기 단계 1)의 동결건조된 고춧잎 추출물 1 중량부에 대하여, 80~120 부피부의 물을 첨가한 후 플라즈마를 처리하는 단계;를 포함하는 피부 미백효과가 증진된 고춧잎 추출물의 제조방법. 1) adding red pepper leaf powder to water, C 1 to C 4 lower alcohol or a mixture thereof to obtain red pepper leaf extract and then lyophilizing; And
2) to about 1 part by weight of the lyophilized red pepper leaf extract of step 1), adding 80 to 120 parts by volume of water and then treating the plasma;
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