KR101988829B1 - Composition for improving skin elasticity and female breast expansion - Google Patents

Composition for improving skin elasticity and female breast expansion Download PDF

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KR101988829B1
KR101988829B1 KR1020180125922A KR20180125922A KR101988829B1 KR 101988829 B1 KR101988829 B1 KR 101988829B1 KR 1020180125922 A KR1020180125922 A KR 1020180125922A KR 20180125922 A KR20180125922 A KR 20180125922A KR 101988829 B1 KR101988829 B1 KR 101988829B1
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이충녕
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주식회사 비바코리아
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783Angiosperms [Magnoliophyta]
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/40Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
    • A61K8/42Amides
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/49Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
    • A61K8/494Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with more than one nitrogen as the only hetero atom
    • A61K8/4953Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with more than one nitrogen as the only hetero atom containing pyrimidine ring derivatives, e.g. minoxidil
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/72Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
    • A61K8/81Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds obtained by reactions involving only carbon-to-carbon unsaturated bonds
    • A61K8/8105Compositions of homopolymers or copolymers of unsaturated aliphatic hydrocarbons having only one carbon-to-carbon double bond; Compositions of derivatives of such polymers
    • A61K8/8111Homopolymers or copolymers of aliphatic olefines, e.g. polyethylene, polyisobutene; Compositions of derivatives of such polymers
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00Preparations for care of the skin
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00Preparations for care of the skin
    • A61Q19/08Anti-ageing preparations
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/85Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine

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Abstract

The present invention relates to a cosmetic composition for female breast enlargement and improvement of skin elasticity and, more specifically, comprises Aureobasidium pullulans fermented by Chinese pea tree, an Angelica tenuissima root extract, an Anemarrhena asphodeloides extract, hydrogenated polyisobutene, adenosine, niacinamide and the like as an active ingredient. The present invention has the advantage of excellent female breast enlargement or skin elasticity improvement effect and excellent skin stability.

Description

여성 가슴 확대 및 피부 탄력 향상용 화장료 조성물{Composition for improving skin elasticity and female breast expansion}{Composition for improving skin elasticity and female breast expansion}

본 발명은 화장료 조성물에 관한 것으로, 더욱 상세하게는 골담초의 흑효모 발효물, 고본 뿌리 추출물, 지모 추출물, 하이드로제네이티드폴리이소부텐, 아데노신 및 나이아신아마이드 등을 유효성분으로 함유함으로써, 여성의 가슴을 확대시키고, 피부 탄력을 증가시키는 화장료 조성물에 관한 것이다.The present invention relates to a cosmetic composition, and more particularly, to a cosmetic composition comprising a black yeast fermented product, a root extract, a gum extract, a hydrogenated polyisobutene, adenosine and niacinamide as active ingredients, Thereby increasing the elasticity of the skin.

여성의 가슴은 흉부의 전면에서 3번째와 7번째 늑골 사이에 위치하고 있는 기관으로 유선(mammary gland), 지방조직(adipose tissue) 및 결합 조직(connective tissue) 등으로 구성되어 있고 근육이 없는 특수한 구조로 이루어져 있다. A woman's chest is located between the third and seventh ribs on the front of the thorax. It is composed of mammary gland, adipose tissue and connective tissue, and has a special structure without muscles. consist of.

이 중 지방 조직은 유방의 볼륨과 강도를 좌우하는 역할을 하며 가슴의 2/3를 구성하고 있는데, 체중의 증감이나 나이, 임신 및 수유 등의 변화 요인에 민감하므로 결국, 이로 인해 야기되는 여성 호르몬 에스트로겐(estrogen)의 분비 감소나 가슴 주변 세포의 발육 약화 등에 의해 가슴의 볼륨이 감소하게 된다. 또한, 나이가 듦에 따라 지방 조직을 둘러싸고 있는 결합조직에서 콜라겐 합성량이 점차 줄고 형태가 변형됨에 따라 가슴 주변 피부가 탄력을 잃고 처지게 된다.Of these, the fat tissue plays a role in the volume and intensity of the breast, making up two-thirds of the chest, and is sensitive to changes in body weight, age, pregnancy and lactation, and consequently, female hormones The volume of the chest is reduced by the decrease of secretion of estrogen or the development of the cells around the chest. In addition, as the age increases, collagen synthesis in the connective tissue surrounding the adipose tissue gradually decreases and the shape changes, so that the skin around the chest loses its elasticity and suffers.

현대 사회에서 여성의 가슴은 건강과 미의 상징으로 인식되고 있으며, 많은 여성들이 부작용과 경제적인 부담이 따르는 성형수술을 통해서라도 모양과 크기 및 탄력을 증진시키고자 할 만큼 여성들에게 민감한 문제로 대두되고 있다. 또한, 폐경기 여성들에게 발생하게 되는 호르몬, 혈액, 지방 및 뼈 등의 대사 이상으로 인한 가슴 피부 탄력 저하 및 지방의 감소는 여성성에 대한 큰 상실감을 느끼게 하므로 바스트 퍼밍 제품의 필요성과 중요성이 어느 때보다 부각되고 있다.In modern society, women's breast is recognized as a symbol of health and beauty, and many women become sensitive to women's desire to promote shape, size and resilience through cosmetic surgery with side effects and economic burdens have. In addition, the lowering of skin elasticity and fat loss caused by metabolic abnormalities such as hormones, blood, fat and bones that occur in postmenopausal women causes a great sense of loss of femininity, so the necessity and importance of Bust Firming products are more emphasized than ever. .

이에 이러한 여성들의 고민을 해결하고자 하는 일환으로, 대한민국 공개특허 제10-2003-0087680호에서는 프럭토오스 1,6-디포스페이트 또는 그 유도체가 지방세포 내의 NADPH(nicotinamide adenine dinucleotide phosphate)의 생성 및 중성 지방(triglyceride)의 생성을 촉진함으로써 지방세포의 분화(differentiation) 촉진 효능이 있으며 인체에 적용 시 가슴둘레를 증가하고 피부의 탄력을 개선 시킴을 밝힌 바가 있다.In order to solve these women's anxieties, Korean Patent Laid-Open No. 10-2003-0087680 discloses that fructose 1,6-diphosphate or a derivative thereof produces nicotinamide adenine dinucleotide phosphate (NADPH) It has been shown that it promotes the differentiation of adipocytes by promoting the production of triglyceride and increases the chest circumference and skin elasticity when applied to human body.

또한, 대한민국 등록특허 제10-1754227호에서는 시트롤린 및 라이신; 및 지모, 소나무, 톱야자 및 레드클로버의 식물 추출물을 유효성분으로 하는 여성의 가슴 확대용 조성물을 제안한 바 있다.In Korean Patent No. 10-1754227, citroline and lysine; And a plant extract of Zymo, Pine, Saw Palmetto and Red Clover as active ingredients.

아울러, 대한민국 등록특허 제10-1825171호에서는 지모 추출물, 하이드로제네이티드폴리이소부텐, 검정콩 추출물, 쌀겨 추출물, 참깨추출물, 아데노신 및 나이아신아마이드를 유효성분으로 여성의 가슴 확대용 조성물을 제안한 바 있다. In addition, Korean Patent No. 10-1825171 has proposed a composition for breast enlargement of women, which comprises as an active ingredient, Zymo extract, hydrogenated polyisobutene, black soybean extract, rice bran extract, sesame extract, adenosine and niacinamide.

KR 10-2003-0087680 AKR 10-2003-0087680 A KR 10-2003-0087680 AKR 10-2003-0087680 A KR 10-1825171 B1KR 10-1825171 B1 KR 10-2011-0023531 AKR 10-2011-0023531 A KR 10-2012-0012904 AKR 10-2012-0012904A KR 10-2015-0041981 AKR 10-2015-0041981 A

본 발명의 목적은, 골담초의 흑효모 발효물, 고본 뿌리 추출물, 지모 추출물, 하이드로제네이티드폴리이소부텐, 아데노신 및 나이아신아마이드를 유효성분으로 함유하여 여성의 가슴을 확대시키고, 피부의 탄력을 향상시키는 화장료 조성물을 제공하는 것이다. It is an object of the present invention to provide a method for improving the elasticity of skin by expanding the chest of a woman and containing the fermented black yeast fermented product of Saccharomyces cerevisiae, gibbosa root extract, Zygo extract, hydroisomerized polyisobutene, adenosine and niacinamide as active ingredients To provide a cosmetic composition.

상기한 목적을 달성하기 위한 본 발명의 여성의 가슴 확대 및 피부 탄력 향상용 화장료 조성물은, 골담초의 흑효모 발효물, 고본 뿌리 추출물, 지모 추출물, 하이드로제네이티드폴리이소부텐, 아데노신 및 나이아신아마이드를 유효성분으로 포함하는 것을 특징으로 한다. In order to achieve the above object, the present invention provides a cosmetic composition for enhancing breast enlargement and skin elasticity of a female, which comprises a fermented black yeast fermented product of Saccharomyces cerevisiae, a root extract of Gochum root, a lipopolysaccharide extract, a hydrogenated polyisobutene, adenosine and niacinamide As a component.

검정콩 추출물, 쌀겨 추출물 및 참깨 추출물을 더 포함하는 것을 특징으로 한다.Black bean extract, rice bran extract and sesame extract.

상기 골담초의 흑효모 발효물은, 골담초에 흑효모(Aureobasidium pullulans)를 접종하고, 20~30℃에서 2~4일간 발효하고, 상기 발효한 발효물에 5~15부피배의 물을 첨가하여 70~80℃에서 2~4시간 동안 추출한 것임을 특징으로 한다. The fermented black yeast fermented product was prepared by inoculating Aureobasidium pullulans into a fermented fermented product at 20 to 30 ° C for 2 to 4 days and adding 5 to 15 times by volume of water to the fermented fermented product, At 80 DEG C for 2 to 4 hours.

전체 조성물 100중량%에 대하여, 상기 골담초의 흑효모 발효물 0.01~2중량%, 상기 고본 뿌리 추출물 0.01~2중량%, 상기 지모 추출물 0.001~1중량%, 상기 하이드로제네이티드폴리이소부텐 0.1~9중량%, 상기 아데노신 0.01~0.1중량%, 상기 나이아신아마이드 1~3중량%, 상기 검정콩 추출물 0.01~1중량%, 상기 쌀겨 추출물 0.01~1중량%, 상기 참깨 추출물 0.01~1중량%를 포함하는 것을 특징으로 한다.0.01 to 2% by weight of the fermented black yeast fermented product of the fermentation broth, 0.01 to 2% by weight of the hypericum root extract, 0.001 to 1% by weight of the chrysanthemum extract, 0.1 to 9% by weight of the hydrogenated polyisobutene, 0.01 to 1% by weight of the adenosine, 0.01 to 1% by weight of the adenosine, 1 to 3% by weight of the niacinamide, 0.01 to 1% by weight of the black soybean extract, 0.01 to 1% .

카보머 0.1~0.3중량%, 디소듐이디티에이 0.01~0.05중량%, 1,2-헥산디올 1~3중량%, 부틸렌글라이콜 3~9중량%, 글리세린 2~4중량, 판테놀 0.1~1중량%, 스테아릴알코올 1~3중량%, 세테아릴올리베이트 1~1.5중량%, 소르비탄올리베이트 0.5~1중량%, 폴리글리세릴-3메칠글루코오스디스테아레이트 0.5~1.5중량%, 비즈왁스 0.5~1.5중량%, 세틸에칠헥사노에이트 0.5~5중량%, 트리에칠헥사노인 3~7중량%, 쉐어버터 2~4중량%, 사이클로메치콘 1~3중량%, 해바라기씨 오일 3~5중량%, 트로메타인 0.1~0.5중량%, 카플릴릴글라이콜 0.05~0.2중량%, 에칠헥실글리세린 0.01~0.1중량%, 오레가노잎추출물 0.001~0.1중량%, 스피어민트추출물 0.001~0.1중량%, 레몬밤추출물 0.001~0.1중량%, 디프로필렌글라이콜 0.001~0.1중량%, 펜틸렌글라이콜 0.001~0.1중량% 및 잔량의 정제수를 더 포함하는 것을 특징으로 한다. 0.1 to 0.3% by weight of carbomer, 0.01 to 0.05% by weight of disodium diethylarene, 1 to 3% by weight of 1,2-hexanediol, 3 to 9% by weight of butylene glycol, 2 to 4% by weight of glycerin, 1 to 3 wt% of stearyl alcohol, 1 to 1.5 wt% of cetearyl olivate, 0.5 to 1 wt% of sorbitan olivate, 0.5 to 1.5 wt% of polyglyceryl-3-methylglucoside distearate, 0.5 to 1.5% by weight of beeswax, 0.5 to 5% by weight of cetyl ethylhexanoate, 3 to 7% by weight of triethyhexanone, 2 to 4% by weight of shea butter, 1 to 3% by weight of cyclomethicone, Wherein the extract of Oregano leaves is in the range of 0.001 to 0.1 wt%, the spearmint extract is in the range of 0.001 to 0.1 wt%, the extract is in the range of 3 to 5 wt%, the tromethone is in the range of 0.1 to 0.5 wt%, the capryliliglycol is in the range of 0.05 to 0.2 wt%, the ethylhexyl glycerin is in the range of 0.01 to 0.1 wt% 0.001 to 0.1% by weight of a lemon balm extract, 0.001 to 0.1% by weight of dipropylene glycol, 0.001 to 0.1% by weight of pentylene glycol, and a remaining amount of purified water.

본 발명에 의하면 여성의 가슴 확대 또는 피부 탄력의 개선 효과가 탁월하고, 피부 안정성이 뛰어나다는 장점이 있다.According to the present invention, the effect of improving breast enlargement or skin elasticity of a woman is excellent, and skin stability is excellent.

도 1은 본 발명에 따른 시험예 1의 결과를 나타낸 그래프.1 is a graph showing the results of Test Example 1 according to the present invention.

이하, 본 발명을 상세히 설명한다.Hereinafter, the present invention will be described in detail.

본 발명은 골담초의 흑효모 발효물, 고본 뿌리 추출물, 지모 추출물, 하이드로제네이티드폴리이소부텐, 아데노신 및 나이아신아마이드를 유효성분으로 포함하는 여성의 가슴 확대용 및 피부 탄력 향상용 조성물을 제공하는 것을 특징으로 한다.The present invention provides a composition for enhancing breast elasticity and skin elasticity of a female, comprising black fermented yeast fermented product of Saccharomyces cerevisiae, high-malt root extract, zygomycetes extract, hydrogenated polyisobutene, adenosine and niacinamide as an active ingredient .

먼저, 상기 골담초(chinese pea tree)의 흑효모 발효물은, 골담초의 잎, 가지, 열매껍질, 열매 중 1종 이상의 것에 흑효모(Aureobasidium pullulans)를 접종하고 20~30℃에서 2~4일간 발효하고, 상기 발효한 발효물에 5~15부피배의 물을 첨가하여 70~80℃에서 2~4시간 동안 추출한 것을 의미하는바, 골담초의 추출물과는 달리 골담초의 흑효모 발효물은, 피부의 지방합성과 축적활성을 현저히 향상시키는 기능을 한다. First, the black yeast fermented product of the chinese pea tree is obtained by inoculating black yeast (Aureobasidium pullulans) into at least one leaf, branch, fruit husk, and fruit of a green tea leaf, fermenting at 20 to 30 ° C for 2 to 4 days And 5 to 15 times by volume of water was added to the fermented fermented product to extract at 70 to 80 ° C for 2 to 4 hours. The fermented product of the black yeast fermented by the fermented black tea, unlike the fermented product of the fermented fermented product, It functions to significantly improve fat synthesis and accumulation activity.

더욱 구체적으로, 상기 골담초는 잎과 가지에 플라보노이드, 열매 껍질에 알칼로이드, 여물지 않은 열매에는 이노시톨 등이 있으며, carpahenol A(resveratrol trimer), (+)-isoampelopson F(resveratrol dimer), caraphenol B(resveratrol dimer), caraphenol C(resveratrol dimer) 등의 올리고스틸벤(oligostilbene) 류를 함유하고 있는 것으로 보고되어 있다. 주요 효능으로는 강장, 이뇨, 류마티즘, 관절염, 대하증, 요통, 급성유선염, 이명, 뼈, 신경통에 효능이 있는 것으로 알려져 있는 식물이다. More specifically, there is a flavonoid in leaves and branches, an alkaloid in fruit shells, and inositol in unfruitful fruits such as carpahenol A (resveratrol trimer), (+) - isoampelopson F (resveratrol dimer), caraphenol B dimer, caraphenol C (resveratrol dimer), and other oligostilbene derivatives. The main efficacy is a plant known to be effective in tonic, diuretic, rheumatism, arthritis, major depression, back pain, acute mastitis, tinnitus, bones, neuralgia.

그리고 상기 흑효모는 맥주, 포도주 등을 만드는데 사용되는 미생물로서, 곰팡이나 버섯 무리이지만 균사가 없고, 광합성능이나 운동성도 가지지 않는다. 본 발명의 기질인 골담초에 접종되는 흑효모(Aureobasidium pullulans)는 입수된 균주를 종배양시켜 제조될 수 있다. 종균은 사용시까지 주기적으로 계대 배양하여 유지할 수 있는데, 바람직한 구체예로서, PDA(Potato Dextrose Agar) 배지 또는 YM 배지에 상기 흑효모를 접종한 후 약 20~40℃ 정도의 온도에서 약 4주 간격으로 계대 배양하여 유지할 수 있다. 본 발명에 적용되는 흑효모(Aureobasidium pullulans)는 시중에서 일반적으로 구매할 수 있는 것을 사용하면 되므로 그 종류에 대해 특별히 제한하지는 않는다. 아울러, 계대 배양되는 흑효모는 계대 배양되는 흑효모의 배양액 일부를 액체 배지에 접종한 후, 이를 회전 진탕 배양하여 액상의 배양물로서 수득할 수 있다. The black yeast is a microorganism used for making beer and wine, and is a fungus or a mushroom herb, but has no mycelium and no photosynthesis or motility. Aureobasidium pullulans which is inoculated to the scallops, which is a substrate of the present invention, can be produced by seed culturing of the obtained strains. As a preferable example, the black seeds are inoculated into a PDA (Potato Dextrose Agar) medium or YM medium at a temperature of about 20 to 40 DEG C at intervals of about 4 weeks It can be maintained by subculturing. The black yeast (Aureobasidium pullulans) to be applied to the present invention is not particularly limited as it can be generally used in the market. In addition, the black yeast to be subcultured can be obtained as a liquid culture by inoculating a liquid culture medium with a part of the culture medium of the black yeast to be subcultured, and then culturing the culture in a rotary shake.

아울러, 발효시 탄소원과 질소원을 더 첨가할 수도 있는 것으로, 이를 제한하지 않으며, 상기 골담초 100중량부에 대하여 흑효모의 배양액 1~5중량부를 접종할 수 있으나, 배양액의 농도에 따라 적절히 조절될 수 있으며, 이는 본 발명의 기술 분야에서 통상의 지식을 가진 자에게 자명한 사항이다. 상기 발효의 조건은 온도 20~40℃, 상대습도 70~90% 및 발효 기간 1~5일인 것이 바람직하다.It is also possible to add 1 to 5 parts by weight of the black yeast culture to 100 parts by weight of the fermentation broth, but it can be suitably adjusted according to the concentration of the fermentation broth. Which are obvious to those skilled in the art. Preferably, the fermentation conditions are a temperature of 20 to 40 ° C, a relative humidity of 70 to 90%, and a fermentation period of 1 to 5 days.

상기 골담초의 흑효모 발효물은 조성물 총량 100중량%에 대하여, 0.01~2중량% 범위로 포함되는 것이 바람직한데, 그 함량이 그 함량이 0.01중량% 미만이면 가슴 확대의 효과가 미미하고, 2중량%를 초과하더라도 더 이상의 증진된 작용효과가 나타나지 않기 때문이다.The fermented black yeast fermented product of the bamboo shoots is preferably contained in the range of 0.01 to 2% by weight based on 100% by weight of the total composition. If the content is less than 0.01% by weight, the effect of breast enlargement is insignificant. %, The further enhanced action effect is not exhibited.

상기 고본 뿌리 추출물(Angelica tenuissima)은, 고본의 뿌리로부터 추출한 추출물을 의미하는바, 피부의 지방합성과 축적활성을 향상시키고, 피부 탄력을 향상시키는 기능을 한다. 그리고 이러한 기능으로 인해 가슴의 처짐 현상 역시 개선할 수 있다.The above-mentioned Angelica tenuissima refers to an extract extracted from the roots of hardwoods, which improves the lipid synthesis and accumulation activity of the skin and improves skin elasticity. And this function can also improve chest sagging.

상기 고본 뿌리 추출물은 조성물 총량 100중량%에 대하여, 0.01~2중량% 범위로 포함되는 것이 바람직한데, 그 함량이 0.01중량% 미만이면 가슴 확대 및 탄력 증진의 효과가 미미하고, 2중량%를 초과하더라도 더 이상의 증진된 작용효과가 나타나지 않기 때문이다.If the content of the extract is less than 0.01% by weight, the effect of breast enlargement and elasticity enhancement is insignificant, and if it exceeds 2% by weight Because no further enhanced action effects are seen.

상기 지모 추출물(Anemarrhena Asphodeloides Root Extract)은 지모의 뿌리줄기로부터 추출한 추출물을 의미하는바, 피부의 지방합성과 축적활성을 향상시키고, 피부 탄력을 향상시키는 기능을 한다. 그리고 이러한 기능으로 인해 가슴의 처짐 현상 역시 개선할 수 있다.  The above-mentioned Anemarrhena Asphodeloides Root Extract refers to an extract extracted from the root stem of Zygomoe which improves the lipid synthesis and accumulation activity of the skin and improves skin elasticity. And this function can also improve chest sagging.

상기 지모 추출물은 조성물 총량 100중량%에 대하여, 0.001~1중량% 범위로 포함되는 것이 바람직한데, 그 함량이 0.001중량% 미만이면 가슴 확대 및 탄력 증진의 효과가 미미하고, 1중량%를 초과하더라도 더 이상의 증진된 작용효과가 나타나지 않기 때문이다.It is preferable that the Zygomycis melliflua extract is contained in the range of 0.001 to 1 wt% with respect to the total amount of the composition. When the content is less than 0.001 wt%, the effect of breast enlargement and elasticity enhancement is insignificant, No further enhanced action effects are exhibited.

상기 하이드로제네이티드폴리이소부텐(Hydrogenated Polyisobutene) 역시 상기 지모 추출물과 함께 지방의 합성과 축적활성을 향상시키는 역할을 한다. Hydrogenated polyisobutene also plays a role in enhancing the synthesis and accumulation activity of fats together with the extracts of P. japonica.

상기 하이드로제네이티드폴리이소부텐은 조성물 총량 100중량%에 대하여, 0.1~9중량%의 범위로 포함되는 것이 바람직한데, 이 범위를 벗어날 경우 그 효과가 미미하기 때문이다.It is preferable that the hydrogenated polyisobutene is contained in an amount of 0.1 to 9% by weight based on 100% by weight of the total amount of the composition. If the amount is outside this range, the effect is insignificant.

상기 아데노신(Adenosine)은 아데닌과 D-리보오스가 결합한 뉴클레오티드 성분으로, 피부 자생력을 회복시켜 피부에 탄력을 강화하는 기능을 한다. Adenosine is a nucleotide component that binds adenine and D-ribose, restoring skin's self-renewal ability and enhancing elasticity to the skin.

상기 아데노신은 조성물 총량 100중량%에 대하여, 0.01~1중량%의 범위로 포함되는 것이 바람직한데, 그 함량이 0.01중량% 미만이면 그 효과가 미미하고, 과량이 되더라도 더 이상의 증진된 작용효과가 나타나지 않기 때문이다. It is preferable that the adenosine is contained in an amount of 0.01 to 1% by weight based on 100% by weight of the total amount of the composition. If the content is less than 0.01% by weight, the effect is insignificant. It is not.

상기 나이아신아마이드(Niacinamide)는 수분의 손실을 막아 피부 건조로 오는 불편함을 없애주고, 피부 장벽을 강화시키는 기능을 하는 것이다.The above-mentioned Niacinamide prevents the loss of moisture, thereby eliminating the inconvenience of drying the skin and enhancing the skin barrier.

상기 나이아신아마이드는 조성물 총량 100중량%에 대하여 1~3중량%로 포함되는 것이 바람직한데, 그 함량이 1중량% 미만이면 그 효과가 미미하고, 과량이 되더라도 더 이상의 증진된 작용효과가 나타나지 않기 때문이다. The niacinamide is preferably contained in an amount of 1 to 3% by weight based on 100% by weight of the total amount of the composition. If the content of the niacinamide is less than 1% by weight, the effect is insignificant. to be.

본 발명의 골담초의 흑효모 발효물, 고본 뿌리 추출물, 지모 추출물, 하이드로제네이티드폴리이소부텐, 아데노신 및 나이아신아마이드를 유효성분으로 포함하는 조성물은 지모 추출물, 하이드로제네이티드폴리이소부텐, 아데노신 및 나이아산 아마이드만을 유효성분으로 포함하는 조성물에 비해 우수한 가슴 확대 효과가 있으며, 피부 탄력 증진 효과 역시 우수하다. The composition comprising the black yeast fermented product of Saccharomyces cerevisiae according to the present invention as an active ingredient, the composition containing the fermented product of Saccharomyces cerevisiae, the root extract of Gooseberry, the extract of Glycyrrhiza radix, the hydroisomeric polyisobutene, the adenosine and the niacinamide as the active ingredient is selected from the group consisting of Glycyrrhiza extract, Hydrogenated Polyisobutene, Compared to a composition containing only amide as an active ingredient, it has excellent breast enlargement effect and also has excellent skin elasticity enhancement effect.

또한, 본 발명의 화장료 조성물은 검정콩 추출물, 쌀겨 추출물, 참깨 추출물를 더 포함할 수 있다. In addition, the cosmetic composition of the present invention may further include black bean extract, rice bran extract, and sesame extract.

상기 검정콩 추출물(Glycine Soja (Soybean) Seed Extract)은 흑태, 서리태, 서목태 등의 콩으로부터 추출한 추출물로, 노화방지 성분을 다량 함유하여 피부의 노화를 방지한다. The black soybean extract (Glycine Soja (Soybean) Seed Extract) is an extract derived from soybeans such as black tea, Seolitae, and Seomyeongtae. It contains a large amount of anti-aging ingredients to prevent aging of the skin.

상기 검정콩 추출물은, 조성물 총량 100중량%에 대하여, 0.01~1중량%의 범위로 포함되는 것이 바람직한데, 그 함량이 0.01중량% 미만이면 노화방지의 효과가 미미하고, 과량이 되더라도 더 이상의 증진된 작용효과가 나타나지 않기 때문이다. It is preferable that the black soybean extract is contained in an amount of 0.01 to 1% by weight based on 100% by weight of the total amount of the composition. If the content is less than 0.01% by weight, the effect of preventing aging is insignificant. This is because the effect is not shown.

상기 쌀겨 추출물(Oryza Sativa (Rice) Bran Extract)은 피부에 윤기를 공급하고, 보습력을 제공하는 성분이다.The rice bran extract (Oryza Sativa (Rice) Bran Extract) is a component that provides skin with shine and provides moisture.

상기 쌀겨 추출물은, 조성물 총량 100중량%에 대하여, 0.01~1중량%의 범위로 포함되는 것이 바람직한데, 그 함량이 0.01중량% 미만이면 그 효과가 미미하고, 과량이 되더라도 더 이상의 증진된 작용효과가 나타나지 않기 때문이다. It is preferable that the rice bran extract is contained in an amount of 0.01 to 1% by weight based on 100% by weight of the total amount of the composition. If the content is less than 0.01% by weight, the effect is insignificant. Is not shown.

상기 참깨 추출물(Sesamum Indicum (Sesame) Seed Extract)은 피부에 컨디셔능을 부여하여 활력 있는 피부 관리에 도움을 주는 성분이다. The Sesamum Indicum (Sesame) Seed Extract is a component that helps to regulate the skin by regulating the skin condition.

상기 참깨 추출물은 조성물 총량 100중량%에 대하여, 0.01~1중량%의 범위로 포함되는 것이 바람직한데, 그 함량이 0.01중량% 미만이면 그 효과가 미미하고, 과량이 되더라도 더 이상의 증진된 작용효과가 나타나지 않기 때문이다. The sesame extract is preferably contained in an amount of 0.01 to 1% by weight based on 100% by weight of the total amount of the composition. If the content is less than 0.01% by weight, the effect is insignificant. Because it does not appear.

한편, 본 발명에서 사용하는 상기 추출물들은 당업계에 잘 알려진 방법으로 이용하여 추출한 것을 모두 포함하는바, 구체적으로는, 각 재료를 건조시켜, 마쇄기로 조분쇄한 후, 추출 용기에 넣고, 추출용매를 가한 후 적절한 온도에서 일정 시간 동안 추출하여 수득할 수 있다. 또한, 상기 추출 후, 추가적으로 여과, 농축 및 건조할 수 있다. Meanwhile, the extracts used in the present invention include those extracted using a method well known in the art. Specifically, each of the materials is dried, pulverized into a crusher, placed in an extraction container, Followed by extraction at a suitable temperature for a certain period of time. Further, after the extraction, it may be further filtered, concentrated and dried.

본 발명에 사용하는 용매로는, 물, 에탄올, 메탄올, 부탄올, 에테르, 에틸아세테이트, 클로로포름 및 이들 유기용매와 물의 혼합용매로 이루어진 군에서 선택될 수 있다. 또한, 상기 추출 용매의 양은 각 재료의 건조 중량의 2 내지 20배로 사용할 수 있다. 나아가, 상기 추출 방법은 열 추출, 환류 냉각 추출, 초음파 추출 등의 추출 방법을 사용할 수 있으며, 상기 추출 온도는 10∼100℃가 바람직하며, 상기 추출 시간은 6∼48시간이다. The solvent used in the present invention may be selected from the group consisting of water, ethanol, methanol, butanol, ether, ethyl acetate, chloroform, and a mixed solvent of these organic solvents and water. The amount of the extraction solvent may be 2 to 20 times the dry weight of each material. Further, the extraction method may be an extraction method such as heat extraction, reflux cooling extraction, ultrasonic extraction, etc. The extraction temperature is preferably 10 to 100 ° C, and the extraction time is 6 to 48 hours.

상기와 같은 본 발명의 조성물은 유화 제형 및 가용화 제형의 화장품으로 제조될 수 있으며, 가장 바람직하게는 크림 타입으로 제조되어 가슴에 도포하는 것이다.The composition of the present invention as described above may be prepared as an emulsified cosmetic or a solubilized cosmetic cosmetic, and most preferably, it is manufactured into a cream type and applied to the chest.

아울러, 본 발명의 화장료 조성물은 용매, 분산제, 점도증진제, 습윤제, 킬레이트제, 유화제, pH 조절제, 방부제, 향료, 각종 컨디셔닝제를 더 포함할 수 있다.In addition, the cosmetic composition of the present invention may further comprise a solvent, a dispersant, a viscosity enhancer, a wetting agent, a chelating agent, an emulsifier, a pH adjuster, a preservative, a flavoring agent and various conditioning agents.

더욱 구체적으로는, 카보머 0.1~0.3중량%, 디소듐이디티에이 0.01~0.05중량%, 1,2-헥산디올 1~3중량%, 부틸렌글라이콜 3~9중량%, 글리세린 2~4중량, 판테놀 0.1~1중량%, 스테아릴알코올 1~3중량%, 세테아릴올리베이트 1~1.5중량%, 소르비탄올리베이트 0.5~1중량%, 폴리글리세릴-3메칠글루코오스디스테아레이트 0.5~1.5중량%, 비즈왁스 0.5~1.5중량%, 세틸에칠헥사노에이트 0.5~5중량%, 트리에칠헥사노인 3~7중량%, 쉐어버터 2~4중량%, 사이클로메치콘 1~3중량%, 해바라기씨 오일 3~5중량%, 트로메타인 0.1~0.5중량%, 카플릴릴글라이콜 0.05~0.2중량%, 에칠헥실글리세린 0.01~0.1중량%, 오레가노잎추출물 0.001~0.1중량%, 스피어민트추출물 0.001~0.1중량%, 레몬밤추출물 0.001~0.1중량%, 디프로필렌글라이콜 0.001~0.1중량%, 펜틸렌글라이콜 0.001~0.1중량% 및 잔량의 정제수를 더 포함할 수 있다.More specifically, it is preferable that 0.1 to 0.3% by weight of carbomer, 0.01 to 0.05% by weight of disodium dithia, 1 to 3% by weight of 1,2-hexanediol, 3 to 9% by weight of butylene glycol, 0.1 to 1 wt% of panthenol, 1 to 3 wt% of stearyl alcohol, 1 to 1.5 wt% of cetearyl olivate, 0.5 to 1 wt% of sorbitan olivate, 0.5 to 1 wt% of polyglyceryl-3-methylglucoside distearate 0.5 0.5 to 5% by weight of cetyl ethylhexanoate, 3 to 7% by weight of triethyhexanone, 2 to 4% by weight of shea butter, 3 to 7% by weight of cyclomethicone 1 to 3 Wherein the extract of Oregano leaves comprises from 0.001 to 0.1 wt.% Of sunflower seed oil, from 3 to 5 wt.% Of sunflower seed oil, of from 0.1 to 0.5 wt.% Of tromethamine, of 0.05 to 0.2 wt.% Of capryliliglycol, of 0.01 to 0.1 wt.% Of ethylhexyl glycerin, 0.001 to 0.1% by weight of a spearmint extract, 0.001 to 0.1% by weight of a lemon balm extract, 0.001 to 0.1% by weight of dipropylene glycol, 0.001 to 0.1% by weight of pentaerythritol and remaining amount of purified water.

여기서, 상기 카보머(Carbomer)는 점도증진제로서 사용되고, 상기 디소듐이디티에이(Disodium EDTA)는 킬레이트제로 사용되며, 상기 1,2-헥산디올(1,2-Hexanediol), 상기 부틸렌글라이콜(Butylene Glycol), 상기 글리세린(Glycerin), 상기 판텐놀(Panthenol)은 습윤제로서 사용된다. 그리고 상기 스테아릴알코올(Stearyl Alcohol)은 유화안정제로서 사용되고, 상기 세테아릴올리베이트(Cetearyl olivate), 상기 소르비탄올리베이트(Sorbitan olivate), 상기 폴리글리세릴-3메칠글루코오스디스테아레이트(Polyglyceryl-3 Methylglucose Distearate), 상기 비즈왁스(Beeswax)는 유화제로서 사용된다. 또한, 상기 세틸에칠헥사노에이트(Cetyl Ethylhexanoate)는 피부유연화제로, 상기 트리에칠헥사노인(Triethylhexanoin)와 상기 쉐어버터(Butyrospermum Parkii (Shea) Butter)는 수분증발 차단제 및 피부 컨디셔닝제로 사용되고, 상기 사이클로메치콘(Cyclomethicone)는 피부 유연화제로 사용되며, 상기 해바라기씨 오일(Helianthus Annuus (Sunflower) Seed Oil)은 수분증발 차단제로, 상기 트로메타인(Tromethamine)은 PH 조절제로 사용된다. 그리고 상기 카플릴릴글라이콜(Caprylyl Glycol)은 피부 유연화제로 사용되고, 상기 에칠헥실글리세린은 피부 컨디셔닝제로 사용되며, 상기 오레가노잎 추출물(Origanum Vulgare Leaf Extract), 상기 스피어민트 추출물(Mentha Viridis (Spearmint) Extract), 상기 레몬밤 추출물(Melissa Officinalis Extract)은 피부 컨디셔닝제 및 노화방지제로서 사용되고, 상기 디프로필렌글라이콜(Dipropylene Glycol)과 상기 펜틸렌글라이콜(Pentylene Glycol)은 보습제로 사용된다.The carbomer is used as a viscosity enhancer and the disodium EDTA is used as a chelating agent. The 1,2-hexanediol, 1,2-hexanediol, (Butylene Glycol), Glycerin, and Panthenol are used as wetting agents. The stearyl alcohol may be used as an emulsifying stabilizer and may be selected from the group consisting of Cetearyl olivate, Sorbitan olivate, Polyglyceryl-3-methylglucosyl distearate, 3 Methylglucose Distearate), and the beeswax (Beeswax) is used as an emulsifier. The above-mentioned Cetyl Ethylhexanoate is a skin softening agent. Triethylhexanoin and Butyrospermum Parkii (Shea Butter) are used as a water evaporation blocking agent and a skin conditioning agent. Cyclomethicone is used as a skin softening agent, and the sunflower seed oil (Helianthus Annuus (Sunflower) Seed Oil) is used as a water evaporation blocking agent, and the tromethamine is used as a PH regulator. The caprylly glycerol is used as a skin softening agent and the ethylhexyl glycerin is used as a skin conditioning agent. The extract of Origanum Vulgare Leaf Extract, the spearmint extract (Mentha Viridis (Spearmint) Extract) , Melissa Officinalis Extract is used as a skin conditioner and an antioxidant, and Dipropylene Glycol and Pentylene Glycol are used as moisturizing agents.

이상과 같이 구성되어 제형화된 화장료 조성물은 사용감이 우수하며, 피부에 안정적이고, 가슴의 확대 및 피부 탄력의 증진 효과가 우수하다는 특징이 있다.The cosmetic composition which is constituted as described above is excellent in feeling of use, is stable to the skin, has an excellent effect of expanding the chest and enhancing skin elasticity.

이하, 본 발명을 구체적인 실시예를 통해 더욱 상세히 설명한다. Hereinafter, the present invention will be described in more detail with reference to specific examples.

(실시예 1)(Example 1)

골담초의 흑효모 발효물 0.5g, 고본 뿌리 추출물 0.5g, 지모 추출물 0.004g, 하이드로제네이티드폴리이소부텐 8.396g, 아데노신 0.04g, 나이아신아마이드 2g을 포함하는 조성물을 제조하였다.A composition was prepared comprising 0.5 g of fermented black yeast fermented with curcuma longifolia, 0.5 g of dried root extract, 0.004 g of ginger extract, 8.396 g of hydrogenated polyisobutene, 0.04 g of adenosine and 2 g of niacinamide.

이때, 각 추출물은 재료를 적절한 크기로 세절하여 충분히 음건하고, 음건이 완료된 재료 100g씩을 취하고, 물 1,000ml을 가하여 100℃에서 환류 추출, 여과 및 동결건조하여 제조하였다.At this time, each extract was prepared by finely shredding the material, shaking enough, taking 100 g of the shrunk material, adding 1,000 ml of water, refluxing at 100 ° C, filtering and freeze-drying.

그리고 상기 골담초의 흑효모 발효물은, 골담초의 잎과 열매껍질을 적절한 크기로 세절하여 충분히 음건하고, 음건이 완료된 잎 50g, 열매껍질 50g, 설탕 5g을 3L 배양 용기에 넣고, 121℃에서 30분간 고압 멸균 후 상온에서 냉각하였다. 그리고 이에 제조된 흑효모의 액체 종균을 5g을 접종하고, 25℃, 상대습도 80%의 배양기에서 72시간 동안 발효시켰다. 발효 후 10 부피배의 물을 첨가하여 70℃에서 2시간 동안 추출한 후, 10,000rpm으로 10분간 원심분리 후 상등액을 취하였으며, 이를 감압하에서 농축 및 동결건조하여 분말로 제조하였다. The fermented black yeast fermented product was prepared by frying the leaf and the fruit husk of the fermented bean curd into an appropriate size and sufficiently shaded. 50 g of the shade-finished leaf, 50 g of the fruit husk and 5 g of sugar were placed in a 3 L culture container, After high pressure sterilization, it was cooled at room temperature. Then, 5 g of the liquid yeast liquid koji produced was inoculated and fermented in an incubator at 25 ° C and 80% relative humidity for 72 hours. After the fermentation, 10 volumes of water was added, and the mixture was extracted at 70 ° C for 2 hours. After centrifugation at 10,000 rpm for 10 minutes, the supernatant was collected and concentrated under reduced pressure and lyophilized to prepare a powder.

이때, 상기 흑효모(Aureobasidium pullulans)는 계대 중인 균주를 사용하여 25℃에서 YM(Yeast extract/[0054] Malt extract, Difco, Co, USA) 배지를 사용하여 배양하여, 4주 간격으로 계대배양하면서, 4℃에 보관하면서 사용하였다. 그리고 500ml 플라스크에 100ml 용량의 액체 배지에 계대 중인 흑효모(Aureobasidium pullulans) 종배양물을 접종한 후, 회전 진탕 배양기(Daihan Labtech CoKorea, 25±1℃, 130rpm)에서 3일간 배양하여 액체 종균으로 사용하였다. The Aureobasidium pullulans were cultured using a strain of YM (yeast extract / Malt extract, Difco, Co, USA) at 25 ° C. in a subculture strain, and subcultured at intervals of 4 weeks , And stored at 4 ° C. Then, a 500 ml flask was inoculated with a sub-culture of Aureobasidium pullulans in a liquid medium of 100 ml capacity, and then cultured for 3 days in a rotary shaking incubator (Daihan Labtech CoKorea, 25 ± 1 ° C., 130 rpm) Respectively.

(실시예 2)(Example 2)

실시예 1과 동일하게 실시하되, The procedure of Example 1 was repeated,

검정콩 추출물 0.02g, 쌀겨 추출물 0.02g, 참깨 추출물 0.02g을 더 포함하는 조성물을 제조하였다.0.02 g of black soybean extract, 0.02 g of rice bran extract, and 0.02 g of sesame extract.

이때, 각 추출물은 재료를 적절한 크기로 세절하여 충분히 음건하고, 음건이 완료된 재료 100g씩을 취하고, 물 1,000ml을 가하여 100℃에서 4시간 환류 추출, 여과 및 동결건조하여 제조하였다.At this time, each extract was prepared by extracting 100 g of the shrunk material, finely shredding the material to a proper size, adding 1,000 ml of water, refluxing for 4 hours at 100 ° C, filtering and freeze-drying.

(시험예 1)(Test Example 1)

3T3-L1 세포(KCLB 구입)를 CO2 인큐베이터 (37℃,5% CO2)에서 10% BS(Bovine serum), 1% PS(penicillin/streptomycin)가 포함된 DMEM 배지를 사용하여 배양하였다. 3~4일 간격으로 계대배양을 시켜가며 실험에 사용하였다. 계대배양으로 지방선구세포(preadipocyte) 상태인 3T3-L1을 이용하여 24 웰 플레이트에 동일한 수로 세포를 시딩(seeding)하였다. 3T3-L1 세포가 웰 플레이트 밑바닥을 덮을 정도로 배양을 한 후, 분화유도 물질인 0.25μM Dex(Dexamethasone), 0.5 mM Mix(Methylisobuthyxanthine)와 10㎍/㎖ Ins.(Insulin)을 첨가한 배양액으로 4일간 배양하여 분화를 유도하였다.3T3-L1 cells (purchased from KCLB) were cultured in DMEM medium containing 10% BS (Bovine serum) and 1% PS (penicillin / streptomycin) in a CO 2 incubator (37 ° C, 5% CO 2 ). The cells were subcultured at 3 to 4 days intervals. Cells were seeded in the same number in 24 well plates using 3T3-L1 preadipocyte state as a subculture. 3T3-L1 cells were cultured to cover the bottom of the well plate and cultured for 4 days with 0.25 μM Dex (Dexamethasone), 0.5 mM Mix (Methylisobuthyxanthine) and 10 μg / ml Ins. (Insulin) And differentiation was induced.

다음으로, 상기 분화유도 배지에 실시예 1과 실시예 2로 되는 시료를 각각 25㎍/㎖ 농도로 첨가하여 배양하였으며, 시료는 배양액을 교환할 때마다 2일 간격으로 3회 처리하였다. 10일간 배양 후 배양액을 제거하고 PBS(Phosphate buffer saline)로 세척, 10% 포말린(formalin)으로 고정시킨 후 Oil Red O를 처리하여 염색하였다. 그리고 염색된 지방들을 이소프로판올에 녹여 96 웰 플레이트에 옮긴 후 490 nm에서 흡광도를 측정하여 지방의 축적양을 측정하고, 이를 통해 상대적인 지방 축적률을 계산하였다. 또한, 상기 각 시료를 2일 간격으로 3회 처리한 이후의 다른 well에 트립신(Trypsin)/EDTA를 처리하여 세포를 분리하고, 트리판 블루(trypan blue)로 처리하여 세포현탁액을 만든 다음 혈구계산판(hemocytometer)을 이용하여 세포수를 측정하여 세포 성장률을 계산하였다.Next, the specimens of Example 1 and Example 2 were added to the differentiation induction medium at a concentration of 25 μg / ml, and the samples were treated three times at intervals of 2 days every time the culture medium was exchanged. After culturing for 10 days, the culture medium was removed, washed with phosphate buffered saline (PBS), fixed with 10% formalin, and stained with Oil Red O treatment. The dyed fats were dissolved in isopropanol and transferred to a 96-well plate. The absorbance at 490 nm was measured to determine the amount of accumulated fat, and the relative fat accumulation rate was calculated. In addition, each of the above samples was treated three times at intervals of 2 days, then treated with trypsin / EDTA to separate the cells, treated with trypan blue to prepare a cell suspension, The cells were counted using a hemocytometer to calculate the cell growth rate.

양성 대조군으로는, 지모 추출물 0.005g, 하이드로제네이티드폴리이소부텐 8.395g, 검정콩 추출물 0.02g, 쌀겨 추출물 0.02g, 참깨 추출물 0.02g, 아데노신 0.04g, 나이아신아마이드 2g을 포함하는 조성물을 사용하였다.As a positive control group, a composition comprising 0.005 g of Zygo extract, 8.395 g of hydrogenated polyisobutene, 0.02 g of black soybean extract, 0.02 g of rice bran extract, 0.02 g of sesame extract, 0.04 g of adenosine and 2 g of niacinamide was used.

도 1은 그 결과를 나타낸 그래프로서, 본 발명의 실시예 1 및 실시예 2는 양성대조군에 비해 지방 축적률 및 지방세포 성장률이 현저히 향상된 것을 확인할 수 있었다. FIG. 1 is a graph showing the results. It was confirmed that the fat accumulation ratio and the fat cell growth rate of Example 1 and Example 2 of the present invention were significantly improved as compared with the positive control group.

(시험예 2)(Test Example 2)

인간유래의 섬유아세포를 세포배양용 24 웰 플레이트(well plate)에 분주하고, 10% FBS(fetal bovine serum)를 함유하는 배지에서 37℃, 5% CO2 조건하에서 24시간 동안 배양한 후 PBS(phosphate buffer saline)로 2회 세척하였다. 이러한 방법에 따라 배양된 섬유아세포와 1% FBS를 함유하는 배지에 상기 실시예 1 및 22의 시료를 최종농도가 0.001w/v%가 되도록 처리한 후, 방사선 동위원소로 표지한 아미노산(2,3-3H proline) 2μCi, 아스콜빈산 30㎍/㎖를 첨가하고, 37℃, 5% CO2 조건에서 24시간 배양한 후 배양을 종료하였다.Human fibroblasts were seeded in a 24-well plate for cell culture and cultured in a medium containing 10% FBS (fetal bovine serum) at 37 ° C and 5% CO 2 for 24 hours. Then, PBS (phosphate buffer saline). The samples of Examples 1 and 22 were treated to a final concentration of 0.001 w / v% in a medium containing fibroblasts cultured according to this method and 1% FBS, and then the radioactive isotope-labeled amino acids (2, 3-3H proline), and 30 μg / ml of ascorbic acid were added to the cells and cultured at 37 ° C. and 5% CO 2 for 24 hours.

배양 종료 후 상기 세포 및 배양액을 각각 1/2씩 나누어 한쪽 분획에만 콜라게나아제를 처리한 후 TCA(trichloroacetic acid)로 단백질을 침전시켜 두 분획의 방사활성의 차이로부터 생합성된 콜라겐의 양을 측정하였다. 각 시료의 콜라겐 생합성 촉진효과는 추출물을 첨가하지 않은 대조군의 것을 100%로 하여 콜라겐 증가율로 표 1에 나타내었다. After completion of the culturing, the cells and the culture medium were divided into ½ portions, and only one fraction was treated with collagenase, and the protein was precipitated with trichloroacetic acid (TCA), and the amount of collagen biosynthesized from the difference in radioactivity between the two fractions was measured . The collagen biosynthesis promoting effect of each sample was shown in Table 1 at a collagen growth rate with 100% of the control group to which the extract was not added.

양성 대조군으로는, 지모 추출물 0.005g, 하이드로제네이티드폴리이소부텐 8.395g, 검정콩 추출물 0.02g, 쌀겨 추출물 0.02g, 참깨 추출물 0.02g, 아데노신 0.04g, 나이아신아마이드 2g을 포함하는 조성물을 사용하였다.As a positive control group, a composition comprising 0.005 g of Zygo extract, 8.395 g of hydrogenated polyisobutene, 0.02 g of black soybean extract, 0.02 g of rice bran extract, 0.02 g of sesame extract, 0.04 g of adenosine and 2 g of niacinamide was used.

시험예 2의 결과.Results of Test Example 2. 구분division 콜라겐 증가율(%)Collagen growth rate (%) 대조군Control group 100100 실시예 1Example 1 247247 실시예 2Example 2 261261 양성 대조군Positive control group 190190

상기 표 1에서 확인할 수 있는 바와 같이, 본 발명에 따른 실시예 1 및 2는 매우 탁월한 콜라겐 생합성 촉진 효과가 있음이 확인되었다.As can be seen from the above Table 1, Examples 1 and 2 according to the present invention were found to have an excellent collagen biosynthesis promoting effect.

상기 실시예 1, 2를 이용하여, 하기 표 2와 같은 성분비로서 다음과 같이 크림 타입의 화장품으로 제형화하였다.Using the above-described Examples 1 and 2, the following ingredients were formulated into cream-type cosmetics as shown in Table 2 below.

제형예 1, 2의 성분비(중량%)Component ratios (% by weight) of Formulation Examples 1 and 2, 구분division 제형예 1Formulation Example 1 제형예 2Formulation Example 2 실시예 1Example 1 11.4411.44 -- 실시예 2Example 2 -- 11.5011.50 카보머Carbomer 0.200.20 0.200.20 디소듐이디티에이Disodium iodide 0.030.03 0.030.03 1,2-헥산디올1,2-hexanediol 2.002.00 2.002.00 부틸렌글라이콜Butylene glycol 5.055.05 5.055.05 글리세린glycerin 3.003.00 3.003.00 판테놀Panthenol 0.500.50 0.500.50 스테아릴알코올Stearyl alcohol 2.002.00 2.002.00 세테아릴올리베이트Cetearyl olivate 1.201.20 1.201.20 소르비탄올리베이트Sorbitan olivate 0.800.80 0.800.80 폴리글리세릴-3메칠글루코오스디스테아레이트Polyglyceryl-3 methyl glucoside distearate 1.001.00 1.001.00 비즈왁스Beads wax 1.001.00 1.001.00 세틸에칠헥사노에이트Cetyl hexanoate 3.003.00 3.003.00 트리에칠헥사노인Triethanolhexanone 5.005.00 5.005.00 쉐어버터Sheer butter 3.003.00 3.003.00 사이클로메치콘Cyclomethicone 2.002.00 2.002.00 해바라기씨오일Sunflower seed oil 4.004.00 4.004.00 트로메타민Tromethamine 0.200.20 0.200.20 카프릴릴글라이콜Caprylic glycol 0.100.10 0.100.10 에칠헥실글리세린Ethylhexyl Glycerin 0.050.05 0.050.05 오레가노잎추출물Oregano leaf extract 0.030.03 0.030.03 스피어민트추출물Spearmint extract 0.020.02 0.020.02 레몬밤추출물Lemon balm extract 0.020.02 0.020.02 디프로필렌글라이콜Dipropylene glycol 0.020.02 0.020.02 펜틸렌글라이콜Pentyleneglycol 0.010.01 0.010.01 정제수Purified water to 100to 100 to 100to 100

(시험예 3)(Test Example 3)

노화로 인하여 가슴 볼륨 감소 및 처짐을 호소하는 여성 10명을 선정하였다. 연령은 연령은 40세에서 50세까지 였으며, 평균 연령은 45.1세였다.Ten women who complained of decreased volume and sagging due to aging were selected. Age ranged from 40 to 50 years, with an average age of 45.1 years.

상기 제형예 1, 2에 따라 제조된 크림을 가슴에 12시간 간격으로 8주간 하루 2회 도포토록 하고, 가슴의 볼륨 증가 정도, 탄력 증가 정도, 처짐 개선 정도를 스스로 평가토록 하였으며, 크림의 사용감 역시 조사하였다.The creams prepared according to Formulation Examples 1 and 2 were applied to the chest at intervals of 12 hours for 8 weeks twice a day to evaluate the degree of volume increase, elasticity increase and sagging improvement of the breast by themselves, Respectively.

평가는 문진을 통해 3: 많이 개선(많이 좋음), 2: 약간 개선(약간 좋음), 1: 차이 없음(보통)으로 응답토록 하였으며, 그 점수를 평균 내어 하기 표 3에 나타내었다. The results were as follows. 3: significantly improved (good), 2: slightly improved (slightly better), 1: no difference (normal), and the scores were averaged.

시험예 3 결과.Test Example 3 Results. 구분division 볼륨 증가Volume increase 탄력 증가Increased elasticity 처짐 개선Improve deflection 사용감Feeling 제형예 1Formulation Example 1 2.22.2 2.22.2 1.71.7 2.42.4 제형예 2Formulation Example 2 2.42.4 2.42.4 1.91.9 2.52.5

상기 표 3에서 확인할 수 있는 바와 같이, 본 발명의 시료는 피시험자의 자가 평가에 있어서도 가슴의 볼륨 증가, 탄력 증가, 처짐 개선의 효과가 있는 것으로 평가되었으며, 사용감 역시 우수한 것으로 평가되었다. As can be seen from the above Table 3, the samples of the present invention were evaluated to have an effect of increasing the volume of the chest, increasing the elasticity and improving the sagging in the self-evaluation of the subject, and the feeling of use was also evaluated as excellent.

이상과 같이, 본 발명은 비록 한정된 실시예와 도면에 의해 설명되었으나, 본 발명은 이것에 의해 한정되지 않으며 본 발명이 속하는 기술 분야에서 통상의 지식을 가진 자에 의해 본 발명의 기술 사상과 아래에 기재될 특허 청구범위의 균등 범위 내에서 다양한 수정 및 변형이 가능함은 물론이다.While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments. It will be understood that various modifications and changes may be made without departing from the scope of the appended claims.

Claims (5)

골담초의 흑효모 발효물, 고본 뿌리 추출물, 지모 추출물, 하이드로제네이티드폴리이소부텐, 아데노신 및 나이아신아마이드를 유효성분으로 포함하고,
검정콩 추출물, 쌀겨 추출물 및 참깨 추출물을 더 포함하며,
상기 골담초의 흑효모 발효물은,
골담초에 흑효모(Aureobasidium pullulans)를 접종하고, 20~30℃에서 2~4일간 발효하고, 상기 발효한 발효물에 5~15부피배의 물을 첨가하여 70~80℃에서 2~4시간 동안 추출한 것임을 특징으로 하는 여성의 가슴 확대 및 피부 탄력 향상용 화장료 조성물.
A black yeast fermented product of coriander, a root extract of Gobon root, a germ extract, a hydrogenated polyisobutene, adenosine and niacinamide as active ingredients,
Black bean extract, rice bran extract and sesame extract,
The fermented black yeast fermented product of the above-
Aureobasidium pullulans is inoculated into the fermented bean curd, fermented at 20 to 30 ° C for 2 to 4 days, added to the fermented fermented product at a rate of 5 to 15 times by volume with water at 70 to 80 ° C for 2 to 4 hours Wherein the cosmetic composition of the present invention is a composition for improving breast elasticity and skin elasticity.
삭제delete 삭제delete 제1항에 있어서,
전체 조성물 100중량%에 대하여,
상기 골담초의 흑효모 발효물 0.01~2중량%, 상기 고본 뿌리 추출물 0.01~2중량%, 상기 지모 추출물 0.001~1중량%, 상기 하이드로제네이티드폴리이소부텐 0.1~9중량%, 상기 아데노신 0.01~0.1중량%, 상기 나이아신아마이드 1~3중량%, 상기 검정콩 추출물 0.01~1중량%, 상기 쌀겨 추출물 0.01~1중량%, 상기 참깨 추출물 0.01~1중량%를 포함하는 것을 특징으로 하는 여성의 가슴 확대 및 피부 탄력 향상용 화장료 조성물.
The method according to claim 1,
On the basis of 100% by weight of the total composition,
0.01 to 2% by weight of the black yeast fermented product of cornelia chinensis, 0.01 to 2% by weight of the hypericum root extract, 0.001 to 1% by weight of the chrysanthemum extract, 0.1 to 9% by weight of the hydrogenated polyisobutene, 0.01 to 0.1% Wherein the composition comprises 1 to 3% by weight of the niacinamide, 0.01 to 1% by weight of the black soybean extract, 0.01 to 1% by weight of the rice bran extract, and 0.01 to 1% by weight of the sesame seed extract. A cosmetic composition for improving skin elasticity.
제4항에 있어서,
카보머 0.1~0.3중량%, 디소듐이디티에이 0.01~0.05중량%, 1,2-헥산디올 1~3중량%, 부틸렌글라이콜 3~9중량%, 글리세린 2~4중량, 판테놀 0.1~1중량%, 스테아릴알코올 1~3중량%, 세테아릴올리베이트 1~1.5중량%, 소르비탄올리베이트 0.5~1중량%, 폴리글리세릴-3메칠글루코오스디스테아레이트 0.5~1.5중량%, 비즈왁스 0.5~1.5중량%, 세틸에칠헥사노에이트 0.5~5중량%, 트리에칠헥사노인 3~7중량%, 쉐어버터 2~4중량%, 사이클로메치콘 1~3중량%, 해바라기씨 오일 3~5중량%, 트로메타인 0.1~0.5중량%, 카플릴릴글라이콜 0.05~0.2중량%, 에칠헥실글리세린 0.01~0.1중량%, 오레가노잎추출물 0.001~0.1중량%, 스피어민트추출물 0.001~0.1중량%, 레몬밤추출물 0.001~0.1중량%, 디프로필렌글라이콜 0.001~0.1중량%, 펜틸렌글라이콜 0.001~0.1중량% 및 잔량의 정제수를 더 포함하는 것을 특징으로 하는 여성의 가슴 확대 및 피부 탄력 향상용 화장료 조성물.
5. The method of claim 4,
0.1 to 0.3% by weight of carbomer, 0.01 to 0.05% by weight of disodium diethylarene, 1 to 3% by weight of 1,2-hexanediol, 3 to 9% by weight of butylene glycol, 2 to 4% by weight of glycerin, 1 to 3 wt% of stearyl alcohol, 1 to 1.5 wt% of cetearyl olivate, 0.5 to 1 wt% of sorbitan olivate, 0.5 to 1.5 wt% of polyglyceryl-3-methylglucoside distearate, 0.5 to 1.5% by weight of beeswax, 0.5 to 5% by weight of cetyl ethylhexanoate, 3 to 7% by weight of triethyhexanone, 2 to 4% by weight of shea butter, 1 to 3% by weight of cyclomethicone, Wherein the extract of Oregano leaves is in the range of 0.001 to 0.1 wt%, the spearmint extract is in the range of 0.001 to 0.1 wt%, the extract is in the range of 3 to 5 wt%, the tromethone is in the range of 0.1 to 0.5 wt%, the capryliliglycol is in the range of 0.05 to 0.2 wt%, the ethylhexyl glycerin is in the range of 0.01 to 0.1 wt% Wherein the composition further comprises 0.001 to 0.1% by weight of a lemon balm extract, 0.001 to 0.1% by weight of dipropylene glycol, 0.001 to 0.1% by weight of pentylene glycol, and a remaining amount of purified water. And cosmetic composition for improving skin elasticity.
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