KR101935126B1 - Apparatus for collecting and preprocessing sample - Google Patents
Apparatus for collecting and preprocessing sample Download PDFInfo
- Publication number
- KR101935126B1 KR101935126B1 KR1020170008612A KR20170008612A KR101935126B1 KR 101935126 B1 KR101935126 B1 KR 101935126B1 KR 1020170008612 A KR1020170008612 A KR 1020170008612A KR 20170008612 A KR20170008612 A KR 20170008612A KR 101935126 B1 KR101935126 B1 KR 101935126B1
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- KR
- South Korea
- Prior art keywords
- bag
- solution bag
- solution
- sample collection
- sample
- Prior art date
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/26—Inoculator or sampler
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
Abstract
Sample collection and pretreatment equipment is provided. A sample collecting and preprocessing apparatus according to an embodiment of the present invention is a sample collecting and preprocessing apparatus for collecting and pre-processing a target sample using an extraction solution, comprising a solution bag holder 100, A solution bag 200 and a sample input rod 300 injected into the solution bag 200.
Description
The present invention relates to a sample collecting and pretreatment apparatus, and more particularly, to a sample collecting and pretreatment apparatus capable of extracting a sample to be analyzed, particularly a biomarker in a snot, and directly applying the sample to a detection kit or the like.
Blood, tissue, and other invasive specimens not only cause patient pain during sampling, but are also uneconomical in terms of time and manpower.
Therefore, non-invasive specimens which do not cause pain to the patient and can be easily sampled in a relatively short time are widely used for diagnosis of diseases and the like, and typical examples are runny nose.
Since the concentration of biomarkers present in the nasal mucosa is very variable depending on the collection method, in order to ensure the accuracy and reproducibility required for the diagnosis, it is necessary to standardize the collection and treatment of the sample and to minimize the loss of the biomarker to be analyzed A sample pretreatment device is needed.
In general, centrifugal separation and extraction methods are used as pretreatment methods for runny nose collected by absorbing material. However, in order to use centrifugation, a separate device and a container are used. Since centrifugation must be performed directly, it is inconvenient to use by the general public, and the process is also complicated, making it difficult to be used for on-site diagnosis or self-diagnosis. In the case of the extraction method using the extraction solution, there may be a problem in the accuracy due to the deterioration of the extraction efficiency in the spontaneous extraction, and in the active extraction through the physical stimulation such as stirring, the variable by the user can not be controlled and it may be difficult to obtain the precision and the reproducibility. In addition, there is a disadvantage in that a separate micro outlet is required to dispense a certain amount of sample with the diagnostic kit after extraction.
Therefore, in order to overcome these disadvantages, it is necessary to develop a standardized form of sample collection and pretreatment device that can easily apply non-invasive specimen of nasal sample to the detection kit easily in the field.
SUMMARY OF THE INVENTION The present invention has been made to solve the above problems.
In particular, the present invention is to provide a sample collecting and pretreatment apparatus capable of collecting samples to be analyzed and extracting the sampled samples and directly applying the extracted samples to the detection kit.
One embodiment of the present invention for solving the above problems is a sample pretreatment apparatus for collecting and pretreating a target sample using an extraction solution, comprising: a
In one embodiment, the
In one embodiment, the
The
In one embodiment, a
The
The
The
In one embodiment,
A
In one embodiment, the
In an exemplary embodiment, a
The
In one embodiment, the material of the collecting
In one embodiment, the
The present invention also relates to a sample pretreatment apparatus for collecting and pretreating a sample of interest using an extraction solution , comprising a
In one embodiment, the
In one embodiment, a
In one embodiment, the
In one embodiment, the first and
A
In one embodiment,
In one embodiment, the
In one embodiment, a
The
In one embodiment, the
A
In one embodiment, the
The present invention also provides a solution bag stand (100; 400) in which a solution bag containing an extraction solution for sample collection and pretreatment is mounted, comprising: a bottom plate on which the solution bag is placed; a cradle frame attached below the bottom plate; A solution bag rest assembly including a press plate mounted on one end of the cradle frame and a release plate mounted on the other end of the cradle frame so as to open the mouth of the solution bag, to provide.
In one embodiment, the pressure plate is rotatably and slidably mounted on the cradle frame, and the cradle frame is rotatably mounted on the cradle frame.
In one embodiment, the baffle plate includes a first latching hook hooked on the inlet side upper portion of the solution bag at the mouth opening of the solution bag, and a second latching hook hooked to the lower portion of the inlet shaft of the solution bag And a second hooking hook.
In one embodiment, the lower surface of the cradle frame includes an inclined surface located at the tip side of the cradle frame, and the inclined surface may be inclined upward toward the tip side of the cradle frame.
The present invention also relates to a solution bag 200 (500) containing an extraction solution for sample collection and pretreatment, comprising a first receiving bag in which the extraction solution is received and a second receiving bag Wherein the first receiving bag and the second receiving bag share an inlet and provide a solution bag having a shorter length of the second receiving bag than a length of the first receiving bag.
In one embodiment, a pair of positioning projections projecting laterally may be formed at a rear end of the first receiving bag.
In one embodiment, the first receiving bag includes a first portion that overlaps the second receiving bag and a second portion that does not overlap the second receiving bag, and the extraction solution is received in the second portion .
According to the sample collection and preprocessing apparatus of the present invention, the collection of the sample, the preprocessing and the application to the detection kit can be performed through a single apparatus. It is not necessary to use a separate detection kit application device such as a sample discharge port equipped with a filtration filter and the possibility of the introduction of external contaminants is reduced because the collection process through the extraction and the application to the detection kit are performed at once in the closed pretreatment device, It is easy to use for the general public, so it can be applied to field diagnosis.
In addition, it is possible to collect various samples to be analyzed (food microorganisms, viruses, environmental pollutants, etc.) as well as representative non-invasive specimens (such as runny nose, saliva, sweat, urine, .
Further, the lower surface of the sample pretreatment device is formed to have an inclined surface, and the sample collected in the detection kit can be applied by its own weight without any operation.
In addition, the threaded guide groove and the sample insertion rod are rotated together with each other, so that the biomarker can be easily extracted by the extraction solution from the sample to be analyzed, which is placed on the sample portion of the sample insertion rod by physical stimulation by friction between the solution bag and the solution bag. In addition, since a certain number of threads are formed, the same number of revolutions are provided when the solution of the sample input rod is input regardless of the user, so that the standardized extraction process can be performed by blocking the possibility of adding the variable by the user . As a result, the reliability of the analysis is improved.
Further, since the solution bag accommodating the pretreated sample can be cut through the sliding of the cutting blade, it is easy to apply it to the detection kit.
1 is a perspective view of a sample collecting and pretreatment apparatus according to an embodiment of the present invention.
FIG. 2 is a view showing a solution bag stand installed in the sample collecting and preprocessing apparatus of FIG. 1;
3 is a cross-sectional view taken along line A-A 'of the solution bag stand of FIG. 2;
FIGS. 4 and 5 are views showing the volume of the absorbed sample and the recovery rate of the protein concentration depending on the material of the sampling part of the sample introduction rod. FIG.
FIGS. 6 and 7 illustrate a process of extracting samples using the sample collection and preprocessing apparatus of FIG.
8 is a perspective view of a sample collection and pretreatment apparatus according to another embodiment of the present invention.
FIG. 9 is a view showing a solution bag rest and solution bag cutter provided in the sample collecting and preprocessing apparatus of FIG. 8. FIG.
FIGS. 10 to 12 are views showing a process of extracting samples using the sample collecting and preprocessing apparatus of FIG. 8. FIG.
1. First In the embodiment Of the sample collection device according to
The configuration of the sample collecting and preprocessing apparatus according to the first embodiment of the present invention will be described in detail with reference to FIGS. 1 to 5. FIG.
Referring to FIG. 1, the sample collecting and preprocessing apparatus according to the first embodiment includes a
Referring to FIG. 2, the solution bag stand 100 includes a
The
In order to apply the pretreated sample to the detection kit, the sample collection and preprocessor must be tilted. And samples collected and pretreated by self-weight can be applied to the detection kit. In the sample collecting and preprocessing apparatus of the first embodiment, the
A
Mounting
The mounting
The
The protruding
Referring to FIG. 3, at least one first hooking
The sample collecting and preprocessing apparatus of the first embodiment further includes a
A
It is preferable that
Referring to FIG. 1, a
The
1, the
In other words, the
The
The
The
In the case of the collecting
Experiments were conducted to see how well the snout is absorbed and recovered depending on the material of the collecting
Polyurethane, small (T2.4xW6.6xl12mm) PE-L: Polyester, large (T2.8xW7xL16.8 mm) PU-S: Polyurethane, small (T2. PU-M: Polyurethane, medium (T4ⅹW3xL13 mm) PU-L: Polyurethane, large (T5ⅹW6ⅹL17 mm) Paper-S: Dental
First, the volume recovery test was conducted using PBS solution. As a result, four materials (Cotton, PE-S, PE-L, and PU-L) with high recovery were selected as shown in FIG.
Next, protein recovery test was performed by adding bovine serum albumin (BSA) protein (0.1 μg / ml, 1 μg / ml) to Cotton, PE-S, PE-L and PU-L. As shown in Fig. 4- (B), PE and PU materials having excellent experimental results were selected.
Next, the BSA concentration of the model protein was changed to 0 ~ 100 μg / ml for PE and PU materials, and then the protein elution solution (PBS, pH 7.2, 1% NP-40) Proteins were extracted from PE and PU materials and protein quantification was performed by BCA assay. As shown in FIG. 5, it was found that the PE (polyester) material had the best recovery rate depending on the protein concentration change.
The
The
The
First, the
By forming the
2. First In the embodiment Of sample collection and sample extraction process of preprocessor
6 and 7, sample processing using the sample collecting and preprocessing apparatus according to the first embodiment will be described in detail.
First, the sample to be analyzed is sampled through the
As shown in FIG. 6- (a), the
Next, as shown in Fig. 6- (b), the force of the urging
Next, as shown in FIG. 6- (c), the
Next, as shown in FIG. 7D, when the
Next, as shown in FIG. 7- (e), the
Next, as shown in FIG. 7- (f), when the tip of the
3. Second In the embodiment Of sample collection and preprocessing equipment
8 and 9, the configuration of the sample collecting and preprocessing apparatus according to the second embodiment of the present invention will be described in detail.
Referring to FIG. 8, the sample collecting and preprocessing apparatus according to the second embodiment includes a
Referring to FIG. 9, the solution bag stand 400 includes a
The
A
The
The
The protruding
Referring to FIG. 8, the opening
The
When the
A
The
9, the
The
The
The
The
4. Second Example Sample collection and sample extraction process of preprocessor
A sample extracting process using the sample collecting and preprocessing apparatus of the second embodiment will be described in detail with reference to FIGS. 10 to 12. FIG.
First, the sample to be analyzed is sampled through the
Next, as shown in FIG. 10- (a), the
Next, as shown in Fig. 10- (b), the force of the
Next, as shown in FIG. 10- (c), the
Next, as shown in Figs. 11- (d) and 11 (e), when the
Next, as shown in FIG. 12- (f), when the
Next, as shown in FIG. 12- (g), when the
While the present invention has been described with reference to exemplary embodiments thereof, it will be understood by those of ordinary skill in the art that various changes in form and details may be made therein without departing from the spirit and scope of the present invention as defined by the following claims. It will be appreciated that embodiments are possible. Accordingly, the scope of protection of the present invention should be determined by the claims.
100: solution bag holder
110: cradle frame
111, 112:
111a, 112a: first mounting groove
111b, 112b: second mounting groove
115: When
115a:
120: presser plate
130:
131: first guide groove
131a, 141a:
132: first hooking hook
140: bottom plate
141: second guide groove
142: second hooking hook
200: Solution Bags
210: first receiving bag
211: Positioning projection
220: second receiving bag
300: Sample input rod
310: input rod body
311: Second thread
320: Collection section
330:
400: solution bag holder
410: cradle frame
411: When
411a: Finger groove
420: bottom plate
421, 422:
423, 424:
430:
431: First release plate
431a: first protrusion
432: 2nd release plate
432a: second projection
434: first guide groove
435: second guide groove
434a, 435a: first thread
500: Solution Bags
510: first receiving bag
511: Positioning projection
520: second receiving bag
600: Sample input rod
610: input rod body
611: Second thread
620:
630:
700: solution bag cutter
710: Cutting blade
720: blade cover
730: housing
731: Sliding groove
740:
Claims (35)
Solution bag stand 100;
A solution bag 200 mounted on the solution bag stand 100; And
And a sample input rod (300) charged into the solution bag (200)
The solution bag stand 100 includes:
A cradle frame 110;
A pressing plate 120 mounted on a distal end of the cradle frame 110 for pressing the sample insertion rod 300 inserted into the solution bag 200; And
(130) mounted at the rear end of the cradle frame (110) and for opening the mouth of the solution bag (200)
Sample collection and pretreatment equipment.
The presser plate 120 and the protrusion plate 130 are made of a silicone material.
Sample collection and pretreatment equipment.
The lower surface 115 of the cradle frame 110 includes an inclined surface on the tip side of the cradle frame 110. The inclined surface is inclined upward toward the tip side of the cradle frame 110,
Sample collection and pretreatment equipment.
The lower surface 115 of the cradle frame 110 is formed with a holding groove 115a in the lateral direction of the cradle frame 110,
Sample collection and pretreatment equipment.
Mounting portions 111 and 112 are formed on the left and right ends of the upper surface of the cradle frame 110,
The first mounting grooves 111a and 112a on which the pressure plate 120 is mounted and the second mounting grooves 111b and 112b on which the pressing plate 130 is mounted are formed on the mounting portions 111 and 112,
Sample collection and pretreatment equipment.
The pressing plate 120 is rotatably mounted to the first mounting grooves 111a and 112a and the first mounting grooves 111a and 112a are formed in the shape of a slot so that the pressing plate 120 can slide,
The baffle plate 130 is rotatably mounted on the second mounting grooves 111b and 112b,
Sample collection and pretreatment equipment.
Further comprising a bottom plate (140) mounted to an upper surface (116) of the cradle frame (110)
A first guide groove 141 is formed in the bottom plate 140,
A second guide groove 131 is formed at the corresponding position of the first guide groove 141,
Sample collection and pretreatment equipment.
First threads 131a and 141a are formed in the first guide groove 141 and the second guide groove 131,
The sample input rod 300 is provided with a second thread 311 engaged with the first threads 131a and 141a,
Sample collection and pretreatment equipment.
A first hooking hook 132 is formed at the opening 130 of the solution bag 200 when the solution bag 200 is opened.
The bottom plate 140 is provided with a second hooking hook 142 which is hooked on a lower portion of the inlet shaft of the solution bag 200 when the solution bag 200 is opened.
Sample collection and pretreatment equipment.
The solution bag 200 includes:
A first receiving bag 210 in which the extraction solution is received; And
And a second receiving bag (220) disposed in the first receiving bag (210) to share an inlet with the first receiving bag (210), the second receiving bag being shorter in length than the first receiving bag (210)
Sample collection and pretreatment equipment.
A positioning protrusion 211 projecting laterally is formed at a rear end of the first receiving bag 210,
Sample collection and pretreatment equipment.
The sample input rod (300)
An input rod body 310;
A collecting part 320 provided at the tip of the charging rod body 310; And
And a grip portion 330 provided at a rear end of the input rod body 310 and thicker than the input rod body 310. [
Sample collection and pretreatment equipment.
The collecting unit 320 may be formed of any one material selected from the group consisting of cotton, paper, polyester, and polyurethane.
Sample collection and pretreatment equipment.
The second thread 311 is formed on the insertion rod body 310,
Sample collection and pretreatment equipment.
Solution bag rest 400;
A solution bag 500 mounted on the solution bag stand 400;
A sample input rod 600 charged into the solution bag 500; And
And a solution bag cutter (700) for cutting the solution bag (500)
The solution bag holder 400 includes:
A cradle frame 410;
A bottom plate 420 mounted on the upper surface of the cradle frame 410; And
(430) mounted at a rear end of the cradle frame (410) for opening the mouth of the solution bag (500)
Sample collection and pretreatment equipment.
The bottom surface 411 of the cradle frame 410 has a holding groove 411a formed in the lateral direction of the cradle frame 410,
Sample collection and pretreatment equipment.
The bulging plate 430,
A first baffle plate 431 having a first latching hook 431a; And
And a second baffle plate (432) having a second latching hook (432a)
The first hooking hook 431a is hooked on the inlet side of the solution bag 500 and the second hooking hook 432a is hooked on the inlet side of the solution bag 500 when the solution bag 500 is opened. The lower portion of the inlet side,
Sample collection and pretreatment equipment.
The first and second protrusions 431 and 432 are hinged to each other,
Sample collection and pretreatment equipment.
A first guide groove 434 is formed in the first protrusion 431,
A second guide groove 435 is formed at the corresponding position of the first guide groove 434 in the second protrusion 432,
Sample collection and pretreatment equipment.
First threads 434a and 435a are formed in the first guide groove 434 and the second guide groove 435,
The sample input rod 600 is provided with a second screw thread 611 engaged with the first screw thread 434a 435a,
Sample collection and pretreatment equipment.
The solution bag 500 includes:
A first receiving bag 510 in which the extraction solution is received; And
And a second receiving bag (520) disposed in the first receiving bag (510) to share an inlet with the first receiving bag (510) and shorter in length than the first receiving bag (510)
Sample collection and pretreatment equipment.
A positioning protrusion 511 protruding laterally is formed at a rear end of the first receiving bag 510,
Sample collection and pretreatment equipment.
The bottom plate 420 is provided with first fixing portions 421 and 422 for fixing the positioning protrusions 511 and second fixing portions 423 and 424 for restricting the solution bag 500 to move up and down and back and forth, Lt; / RTI &
Sample collection and pretreatment equipment.
The solution bag cutter (700)
A cutting blade 710 for cutting the tip of the solution bag 500;
A blade cover 720 covering a portion of the cutting blade 710; And
And a housing (730) for receiving the blade cover (720).
Sample collection and pretreatment equipment.
A sliding groove 731 for sliding the blade cover 720 is formed in the housing 730,
The tip of the solution bag 500 is cut by the sliding of the blade cover 720,
Sample collection and pretreatment equipment.
The solution bag cutter 700 is hinged to the solution bag holder 400 and is hinged to the solution bag holder 400 and is rotatable about the connection 740 by a predetermined angle,
Sample collection and pretreatment equipment.
A bottom plate on which the solution bag is placed;
A cradle frame attached to a lower side of the bottom plate;
A pressure plate mounted on one end of the cradle frame so as to press a portion of the solution bag in which the extraction solution is received; And
And a bulging plate mounted on the other end of the cradle frame so as to open the mouth of the solution bag.
Solution bag holder.
The press plate is rotatably and slidably mounted on the cradle frame,
Wherein the opening plate is rotatably mounted on the cradle frame,
Solution bag holder.
Wherein,
A first latching hook which is hooked on an inlet side upper side of the solution bag at an opening of the solution bag; And
And a second hooking hook that is hooked to a lower portion of an inlet axis of the solution bag at an inlet opening of the extraction solution bag.
Solution bag holder.
Wherein the lower surface of the cradle frame includes an inclined surface located at the tip side of the cradle frame, the inclined surface is inclined upward toward the tip side of the cradle frame,
Solution bag holder.
A first receiving bag in which the extraction solution is received; And
And a second receiving bag disposed in the first receiving bag so as to overlap with a portion of the first receiving bag,
Wherein the first receiving bag and the second receiving bag share an inlet, and the length of the second receiving bag is shorter than the length of the first receiving bag,
Solution Bags.
A pair of positioning projections projecting laterally are formed at a rear end of the first receiving bag,
Solution Bags.
The first bag (1)
A first portion overlapping the second receiving bag; And
And a second portion that does not overlap the second receiving bag,
Said extraction solution being contained within said second portion,
Solution Bags.
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
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KR1020170008612A KR101935126B1 (en) | 2017-01-18 | 2017-01-18 | Apparatus for collecting and preprocessing sample |
PCT/KR2018/000814 WO2018135863A1 (en) | 2017-01-18 | 2018-01-17 | Sample collecting and preprocessing device |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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KR1020170008612A KR101935126B1 (en) | 2017-01-18 | 2017-01-18 | Apparatus for collecting and preprocessing sample |
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KR20180085215A KR20180085215A (en) | 2018-07-26 |
KR101935126B1 true KR101935126B1 (en) | 2019-03-18 |
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WO (1) | WO2018135863A1 (en) |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
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KR100747412B1 (en) | 2003-11-13 | 2007-08-07 | 주식회사유한양행 | A diagnostic kit for prostatic cancer and prostatic hyperlasi using immunochromatography |
KR100854389B1 (en) | 2007-02-28 | 2008-08-26 | 주식회사 아이센스 | Electrochemical biosensor |
JP2009150756A (en) | 2007-12-20 | 2009-07-09 | Seiko Epson Corp | Biological substance detection cartridge, biological substance detecting apparatus, and biological substance detection method |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2009018473A1 (en) | 2007-07-31 | 2009-02-05 | Micronics, Inc. | Sanitary swab collection system, microfluidic assay device, and methods for diagnostic assays |
ES2607219T3 (en) | 2011-07-15 | 2017-03-29 | Orasure Technologies, Inc. | Sample Collection Equipment |
KR101363030B1 (en) * | 2011-08-09 | 2014-02-13 | 조원창 | Apparatus for point of care test of diagnostic kit |
KR20140005535A (en) * | 2012-07-04 | 2014-01-15 | 한국과학기술원 | Fmd virus early detection kit |
KR101593552B1 (en) * | 2014-04-14 | 2016-02-15 | (주)나비바이오텍 | Test device and method for diagnosis of pregnancy using by urine |
-
2017
- 2017-01-18 KR KR1020170008612A patent/KR101935126B1/en active IP Right Grant
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2018
- 2018-01-17 WO PCT/KR2018/000814 patent/WO2018135863A1/en active Application Filing
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR100747412B1 (en) | 2003-11-13 | 2007-08-07 | 주식회사유한양행 | A diagnostic kit for prostatic cancer and prostatic hyperlasi using immunochromatography |
KR100854389B1 (en) | 2007-02-28 | 2008-08-26 | 주식회사 아이센스 | Electrochemical biosensor |
JP2009150756A (en) | 2007-12-20 | 2009-07-09 | Seiko Epson Corp | Biological substance detection cartridge, biological substance detecting apparatus, and biological substance detection method |
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KR20180085215A (en) | 2018-07-26 |
WO2018135863A1 (en) | 2018-07-26 |
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