KR101879953B1 - Composition for protecting skin against UV comprising barley extract as active ingredient - Google Patents
Composition for protecting skin against UV comprising barley extract as active ingredient Download PDFInfo
- Publication number
- KR101879953B1 KR101879953B1 KR1020160153172A KR20160153172A KR101879953B1 KR 101879953 B1 KR101879953 B1 KR 101879953B1 KR 1020160153172 A KR1020160153172 A KR 1020160153172A KR 20160153172 A KR20160153172 A KR 20160153172A KR 101879953 B1 KR101879953 B1 KR 101879953B1
- Authority
- KR
- South Korea
- Prior art keywords
- skin
- composition
- barley
- lotion
- extract
- Prior art date
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- A61K8/00—Cosmetics or similar toiletry preparations
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
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- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
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- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
- A23V2200/318—Foods, ingredients or supplements having a functional effect on health having an effect on skin health and hair or coat
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Abstract
본 발명은 보리 추출물 또는 이의 분획물을 유효성분으로 포함하는 자외선에 대한 피부 보호용 또는 피부 광노화 방지용 화장료 조성물에 관한 것으로서, 상기 보리 추출물은 보리를 3일 내지 6일 동안 발아시킨 후 추출하는 것이고, 상기 조성물은 자외선에 의한 피부세포 손상을 억제하는데 효과가 있어 화장품분야에서 유용하게 활용될 수 있다. The present invention relates to a cosmetic composition for preventing skin or protecting skin against UV rays containing an extract of barley or a fraction thereof as an active ingredient, wherein the barley extract is obtained by germinating barley for 3 to 6 days and then extracting the composition Is effective in inhibiting skin cell damage caused by ultraviolet rays, and thus can be usefully used in the field of cosmetics.
Description
본 발명은 보리 추출물 또는 이의 분획물을 유효성분으로 포함하는 자외선에 대한 피부 보호용 또는 피부 광노화 방지용 화장료 조성물에 관한 것이다. The present invention relates to a cosmetic composition for preventing skin or protecting skin against UV rays containing an extract of Barley or a fraction thereof as an active ingredient.
피부는 인체에서 가장 큰 장기로서 표피층, 진피층 및 피하조직으로 구성되어 있으며 인체의 내부와 외부환경 사이의 장벽 역할을 수행한다. 기본 신진대사를 통해 외부로부터 체내를 보호하고 항상성을 유지하는 중요한 기능을 담당하고 있다. 즉 피부는 인체의 가장 바깥부분에서 화학적, 물리적, 생물학적 피부 장벽기능을 통한 인체보호작용, 체온조절작용, 자극감지작용, 에지 저장 작용, 분비 및 흡수 작용, 비타민 D 합성 등의 많은 역할을 수행하고 있다. 반면 지속적인 스트레스 및 외부로부터의 자극에 반복하여 노출될 경우 1차적으로 가장 손상 받기 쉬운 부분이기도 하다. 최근 전 세계적으로 건강이나 피부 미용에 대한 관심이 증대되면서 노화에 관한 연구가 활발히 진행되고 있다. 특히 야외활동으로 인하여 피부가 태양광선에 그대로 노출되면 자외선에 의해 피부노화가 진행되면서 색소침착과 같은 피부손상을 받게 된다. 이러한 태양광선에 의한 노화를 광노화라고 하며, 특히 UVB와 같은 자외선은 피부조직 내 활성 산소를 형성시켜, 피부에 존재하는 항산화 물질을 감소시킴으로써 산화적 스트레스를 유발시키는 것으로 알려져 있다. 또한 이러한 과정에서 생성된 활성 산소종(free radical)들은 멜라닌 생성을 촉진하고 교원섬유와 탄력섬유의 합성을 감소시켜 주름을 생성시키는 것으로 알려져 있으며, 각질층의 수분 함량을 감소시켜 DNA조직에 손상을 야기하며, 체내의 단백질, 지방, 세포막, 핵산 등을 산화시켜 피부 노화를 더욱 더 촉진시키는 것으로 보고되고 있다. 따라서 환경적 스트레스로부터 피부세포를 보호하고 광노화를 억제하기 위해서는 산화적 스트레스로부터 활성산소를 제거할 수 있는 인체에 대한 안전성이 높은 천연유래 성분으로부터 유용한 항산화 물질의 탐색이 필요하다.Skin is the largest organ in the human body and consists of the epidermis, dermis, and subcutaneous tissue, and acts as a barrier between the internal and external environment of the body. It plays an important role in protecting the body from the outside through the basic metabolism and maintaining the homeostasis. In other words, the skin performs many roles in the outermost part of the body such as human body protection function through chemical, physical and biological skin barrier function, body temperature regulating action, stimulus sensing action, edge storage action, secretion and absorption action, vitamin D synthesis have. On the other hand, repeated exposure to sustained stress and external stimuli is primarily the most vulnerable part. Recently, research on aging has been actively carried out as interest in health and skin beauty has increased in the world. Especially when the skin is exposed to the sunlight due to outdoor activities, skin aging is progressed by ultraviolet rays and skin damage such as pigmentation is caused. Such aging by sunlight is called photoaging, and ultraviolet rays such as UVB, in particular, are known to induce oxidative stress by forming active oxygen in the skin tissue and reducing antioxidant substances present in the skin. In addition, the free radicals generated in this process are known to promote melanin production and reduce the synthesis of collagen fibers and elastic fibers to produce wrinkles. It is believed that the moisture content of the stratum corneum is reduced, And it is reported that the protein, fat, cell membrane, nucleic acid and the like in the body are oxidized to further promote skin aging. Therefore, in order to protect skin cells from environmental stress and inhibit photoaging, it is necessary to search for useful antioxidants from natural ingredients having high safety for human body which can remove active oxygen from oxidative stress.
보리는 세계 4대 작물의 하나이며, 우리나라의 경우, 오곡(쌀, 보리, 조, 콩, 기장) 중 하나이며, 쌀 다음가는 주식 곡물이다. 또한, 예로부터 혼반용 또는 보리밥의 형태로 활용되어 왔을 뿐만 아니라 보리차, 엿기름, 된장, 미숫가루 등 다양한 형태로 가공되어 이용되어진 친숙한 작물이며, 단백질, 지방 및 무기질 등의 영양성분을 고르게 함유하고 있어 영양학적으로도 매우 우수하다. 다양한 생리활성 물질이 풍부하게 들어 있음에도 불구하고, 보리에 대한 연구는 식품으로의 연구가 대부분이었으며, 기능성이 향상된 보리 및 발아 보리에 대한 효능 검증 연구는 매우 미약한 실정이다.Barley is one of the four major crops in the world. In Korea, it is one of the grains (rice, barley, joe, bean, millet), followed by rice grains. In addition, it has been used in the form of mixed rice or barley rice for a long time, and it is a familiar crop that has been processed and used in various forms such as barley tea, malt, soybean paste, and soybean powder, and contains nutrients such as proteins, fats and minerals It is also very nutritious. Despite the abundance of various physiologically active substances, research on barley has been mostly conducted on food, and studies on the efficacy of barley and germinated barley with improved function have been very weak.
발아는 식물 종자의 씨눈과 배젖에 있는 비활성 상태의 DNA 유전 정보와 각종 효소 및 영양소 등이 외적 환경 여건이 좋아지면 활성화되어 싹이 트는 것으로, 씨눈 부분이 발아되면서 단백질과 아미노산, 지방산, 탄수화물, 비타민, 미네랄, 식이섬유 등이 변화하며, γ-oryzanol이나 arabinoxylan, GABA, vitaminE 등의 생리활성 성분들도 증가하고 발아 중에 효소가 활성화됨으로써 영양성분들의 체내 흡수가 용이하게 되는 것으로 알려져 있어 곡류를 비롯한 많은 식품 원료에 대한 발아에 대한 연구가 진행되고 있다. Germination is activated by sprouting when inactivated DNA genetic information and various enzymes and nutrients in seeds and endosperm of plant seeds are improved in external environmental condition. Protein, amino acid, fatty acid, carbohydrate, vitamins , Minerals, and dietary fiber. It is known that physiologically active ingredients such as γ-oryzanol, arabinoxylan, GABA, and vitamin E are increased, and enzymes are activated during germination, thereby facilitating absorption of nutrients into the body. Studies on the germination of food ingredients are under way.
일부 합성 화장품 원료들은 효능이 미진하거나 피부 부작용을 유발하는 등의 여러 가지 문제점이 있다. 따라서 피부 부작용을 유발하지 않으면서 피부 노화 방지 효과를 갖는 원료에 대한 연구가 필요하다. 본 연구에서는 UVB에 의한 피부 광노화로부터 피부 보호 효과가 증가 된 보리 추출물을 제조하고, 피부노화 예방 및 기능성화장품 원료로서의 이용 가능성을 알아보았으며, 그 결과 보리를 식품으로서의 이용 뿐 아니라 피부노화방지 화장품과 관련한 기능성 신소재로도 활용 가능성을 보여 주었다.Some synthetic cosmetic raw materials have various problems such as insufficient efficacy and skin side effects. Therefore, it is necessary to study a raw material having an anti-aging effect without inducing skin side effects. In this study, barley extracts with increased skin protection effect from UVB - induced skin aging were investigated and their potential for skin aging prevention and functional cosmetic ingredients were investigated. As a result, barley was used as food, But also showed usability as a functional new material.
본 발명의 목적은 보리 추출물 또는 이의 분획물을 유효성분으로 포함하는 자외선에 대한 피부 보호용 또는 피부 광노화 방지용 화장료 조성물을 제공하는 것이다.It is an object of the present invention to provide a cosmetic composition for protecting skin or preventing skin photoaging against ultraviolet rays comprising a barley extract or a fraction thereof as an active ingredient.
본 발명의 또 다른 목적은 보리 추출물 또는 이의 분획물을 유효성분으로 포함하는 자외선에 대한 피부 보호용 또는 피부 광노화 방지용 식품 조성물을 제공하는 것이다.It is still another object of the present invention to provide a skin composition for skin protection or anti-aging skin against UV light, which comprises a barley extract or a fraction thereof as an active ingredient.
본 발명의 또 다른 목적은 보리 추출물 또는 이의 분획물을 유효성분으로 포함하는 자외선에 대한 피부 보호용 또는 피부 광노화 방지용 약학 조성물을 제공하는 것이다.It is still another object of the present invention to provide a pharmaceutical composition for skin protection or skin photoaging prevention against ultraviolet rays comprising a barley extract or a fraction thereof as an active ingredient.
상기 목적을 달성하기 위하여, 본 발명은 보리 추출물 또는 이의 분획물을 유효성분으로 포함하는 자외선에 대한 피부 보호용 또는 피부 광노화 방지용 화장료 조성물을 제공한다. In order to achieve the above object, the present invention provides a cosmetic composition for preventing skin or protecting skin from UV rays, which comprises a barley extract or a fraction thereof as an active ingredient.
본 발명의 일실시예에 있어서, 상기 보리 추출물은 보리를 3일 내지 6일 동안 발아시킨 후 추출하는 것일 수 있다. In one embodiment of the present invention, the barley extract may be obtained by germinating barley for 3 to 6 days and then extracting it.
본 발명의 일실시예에 있어서, 상기 보리 추출물은 물, 메탄올, 에탄올, 프로판올, 이소프로판올, 부탄올, 아세톤, 에테르, 벤젠, 클로로폼, 에틸아세테이트, 메틸렌클로라이드, 헥산 및 시클로헥산으로 이루어진 그룹에서 선택되는 어느 하나 이상의 용매 추출물인 것일 수 있다. In one embodiment of the present invention the barley extract is selected from the group consisting of water, methanol, ethanol, propanol, isopropanol, butanol, acetone, ether, benzene, chloroform, ethyl acetate, methylene chloride, hexane and cyclohexane And may be any one or more solvent extracts.
본 발명의 일실시예에 있어서, 상기 분획물은 헥산(hexane) 분획물, 클로로폼(chloroform) 분획물, 에틸아세테이트(ethyl acetate) 분획물, 부탄올(butanol) 분획물 및 물(water) 분획물로 이루어진 그룹에서 선택되는 어느 하나인 것일 수 있다. In one embodiment of the present invention, the fraction is selected from the group consisting of hexane fraction, chloroform fraction, ethyl acetate fraction, butanol fraction and water fraction It can be either.
본 발명의 일실시예에 있어서, 상기 조성물은 자외선에 의한 피부세포 손상을 억제하는 것일 수 있다. In one embodiment of the present invention, the composition may inhibit skin cell damage due to ultraviolet light.
본 발명의 일실시예에 있어서, 상기 추출물 또는 분획물은 화장료 조성물 총 중량에 대하여 0.0001 내지 20 중량부인 것이나, 이에 제한되는 것은 아니다. In one embodiment of the present invention, the extract or fraction is 0.0001 to 20 parts by weight based on the total weight of the cosmetic composition, but is not limited thereto.
본 발명의 일실시예에 있어서, 상기 조성물은 피부외용연고, 크림, 유연화장수, 영양화장수, 팩, 에센스, 젤, 스킨로션, 스킨소프너, 스킨토너, 아스트린젠트, 로션, 밀크 로션, 모이스처 로션, 영양로션, 마사지 크림, 영양크림, 모이스처 크림, 핸드 크림, 파운데이션, 영양에센스, 선스크린, 비누, 클렌징폼, 클렌징로션, 클렌징크림, 바디 로션 및 바디 클렌저로 이루어진 그룹에서 선택되는 어느 하나의 제형으로 제조된 것일 수 있다. In one embodiment of the present invention, the composition may be at least one selected from the group consisting of ointments, creams, softening agents, nutritional lotions, packs, essences, gels, skin lotions, skin softeners, skin toners, astringents, lotions, milk lotions, It is made into one of the formulations selected from the group consisting of lotion, massage cream, nutritional cream, moisturizing cream, hand cream, foundation, nutrition essence, sunscreen, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser .
또한, 본 발명은 보리 추출물 또는 이의 분획물을 유효성분으로 포함하는 자외선에 대한 피부 보호용 또는 피부 광노화 방지용 식품 조성물을 제공한다. The present invention also provides a food composition for skin protection or anti-aging skin against ultraviolet rays comprising an extract of barley or a fraction thereof as an active ingredient.
또한, 본 발명은 보리 추출물 또는 이의 분획물을 유효성분으로 포함하는 자외선에 대한 피부 보호용 또는 피부 광노화 방지용 약학 조성물을 제공한다. In addition, the present invention provides a pharmaceutical composition for skin protection or skin photoaging prevention against ultraviolet rays comprising a barley extract or a fraction thereof as an active ingredient.
본 발명에 따른 보리 추출물 또는 이의 분획물은 자외선에 의한 피부세포 손상을 억제함으로써 자외선에 대한 피부 보호 효과 및 광노화 방지 효과가 우수하여 화장품분야에서 유용하게 활용될 수 있다. The barley extract or its fractions according to the present invention are excellent in skin protection effect and photoaging prevention effect against ultraviolet rays by inhibiting skin cell damage caused by ultraviolet rays, and thus can be usefully used in the field of cosmetics.
도 1은 다한 품종 보리 추출물의 발아기간에 따른 HS68 세포에 대한 세포 독성 평가 결과이다.
도 2는 흑누리 품종 보리 추출물의 발아기간에 따른 HS68 세포에 대한 세포 독성 평가 결과이다.
도 3은 보석찰 품종 보리 추출물의 발아기간에 따른 HS68 세포에 대한 세포 독성 평가 결과이다.
도 4는 흑광 품종 보리 추출물의 발아기간에 따른 HS68 세포에 대한 세포 독성 평가 결과이다.
도 5는 다한 품종 보리 추출물의 발아기간에 따른 UVB로 유도한 피부 광노화 보호 효과 결과이다.
도 6은 흑누리 품종 보리 추출물의 발아기간에 따른 UVB로 유도한 피부 광노화 보호 효과 결과이다.
도 7은 흑광 품종 보리 추출물의 발아기간에 따른 UVB로 유도한 피부 광노화 보호 효과 결과이다.
도 8은 보석찰 품종 보리 추출물의 발아기간에 따른 UVB로 유도한 피부 광노화 보호 효과
도 9는 품종별 보리 추출물의 발아기간에 따른 UVB로 유도한 피부 광노화 보호 효과 결과이다.
도 10은 다한 품종 보리 추출물 및 이의 분획물의 HS68 세포에 대한 세포 독성 평가 결과이다.
도 11은 다한 품종 보리 추출물 및 분획물의 UVB로 유도한 피부 광노화 보호 효과 결과이다. FIG. 1 shows the results of cytotoxicity of HS68 cells according to germination period of various barley extracts.
FIG. 2 shows the results of cytotoxicity of HS68 cells according to the germination period of the barley extract of black rill variety.
FIG. 3 shows the results of cytotoxicity of HS68 cells according to germination period of barley extract of jewelery.
Fig. 4 shows the cytotoxicity of HS68 cells according to the germination period of the barley extract of black rice cultivars.
FIG. 5 shows UVB-induced skin photoaging protection effects of germinating barley extracts of different cultivars.
Fig. 6 shows the results of UVB-induced skin photoaging protection according to the germination period of the barley extract of black rye variety.
Fig. 7 is a result of UVB-induced skin photoaging protection effect of germinating barley extracts of black rice cultivars.
Fig. 8 shows UVB-induced protection of skin photo-aging by germination period of barley extract
FIG. 9 shows UVB-induced protection of skin photo-aging according to the germination period of barley extracts according to the kind.
Fig. 10 shows the results of cytotoxicity evaluation of HS68 cells of different cultivar barley extracts and fractions thereof.
Fig. 11 shows UVB-induced skin photoaging protection effects of barley extracts and fractions of different cultivars.
본 발명에 따른 화장료 조성물에 있어서, 상기 자외선에 대한 피부 보호용 또는 피부 광노화 방지용 화장료 조성물은 보리 추출물 또는 분획물을 상기 조성물 총 중량에 대하여 0.0001~90 중량부의 양으로 함유할 수 있고, 바람직하게는 0.0001~20 중량부일 수 있으나, 이에 제한되지 않는다.In the cosmetic composition according to the present invention, the cosmetic composition for skin protection or skin photoaging against ultraviolet rays may contain an extract of barley or a fraction thereof in an amount of 0.0001 to 90 parts by weight, But it is not limited thereto.
본 발명에 따른 자외선에 대한 피부 보호용 또는 피부 광노화 방지용 화장료 조성물은 피부외용연고, 크림, 유연화장수, 영양화장수, 팩, 에센스, 헤어토닉, 샴푸, 린스, 헤어 컨디셔너, 헤어 트리트먼트, 젤, 스킨로션, 스킨소프너, 스킨토너, 아스트린젠트, 로션, 밀크로션, 모이스처 로션, 영양로션, 마사지 크림, 영양크림, 모이스처 크림, 핸드 크림, 파운데이션, 영양에센스, 선스크린, 비누, 클렌징폼, 클렌징로션, 클렌징크림, 바디 로션 및 바디 클렌저로 이루어지는 군으로부터 선택된 제형을 가질 수 있으며, 이에 제한되지 않는다. 이들 각 제형의 조성물은 그 제형의 제제화에 필요하고 적절한 각종의 기제와 첨가물을 함유할 수 있으며, 이들 성분의 종류와 양은 당업자에 의해 용이하게 선정될 수 있다.The cosmetic composition for preventing skin or protecting skin against UV rays according to the present invention can be used as an external ointment for skin, a cream, a softening agent, a nutritional lotion, a pack, an essence, a hair tonic, a shampoo, a rinse, a hair conditioner, a hair treatment, , Skin Skin Toner, Skin Toner, Astringent, Lotion, Milk Lotion, Moisture Lotion, Nutrition Lotion, Massage Cream, Nourishing Cream, Moisture Cream, Hand Cream, Foundation, Nutrition Essence, Sunscreen, Soap, Cleansing Foam, Cleansing Lotion, Cleansing Cream , Body lotions, and body cleansers, but is not limited thereto. The composition of each of these formulations may contain various kinds of bases and additives necessary for formulation of the formulation, and the kinds and amounts of these ingredients can be easily selected by those skilled in the art.
본 발명의 제형이 페이스트, 크림 또는 겔인 경우에는 담체 성분으로서 동물섬유, 식물섬유, 왁스, 파라핀, 전분, 트라칸트, 셀룰로오스 유도체, 폴리에틸렌 글리콜, 실리콘, 벤토나이트, 실리카, 탈크 또는 산화아연 등이 이용될 수 있다.When the formulation of the present invention is a paste, cream or gel, animal fiber, plant fiber, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .
본 발명의 제형이 파우더 또는 스프레이인 경우에는 담체 성분으로서 락토스, 탈크, 실리카, 알루미늄 히드록시드, 칼슘 실리케이트 또는 폴리아미드 파우더가 이용될 수 있고, 특히 스프레이인 경우에는 추가적으로 클로로플루오로히드로카본, 프로판/부탄 또는 디메틸 에테르와 같은 추진체를 포함할 수 있다.When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.
본 발명의 제형이 용액 또는 유탁액의 경우에는 담체 성분으로서 용매, 용매화제 또는 유탁화제가 이용되고, 예컨대 물, 에탄올, 이소프로판올, 에틸 카보네이트, 에틸 아세테이트, 벤질 알코올, 벤질 벤조에이트, 프로필렌글리콜, 1,3-부틸글리콜 오일, 글리세롤 지방족 에스테르, 폴리에틸렌 글리콜 또는 소르비탄의 지방산 에스테르가 있다.In the case of the solution or emulsion of the present invention, a solvent, a solvent or an emulsifier is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.
본 발명의 제형이 현탁액인 경우에는 담체 성분으로서 물, 에탄올 또는 프로필렌 글리콜과 같은 액상 희석제, 에톡실화 이소스테아릴 알코올, 폴리옥시에틸렌 소르비톨 에스테르 및 폴리옥시에틸렌 소르비탄 에스테르와 같은 현탁제, 미소결정성 셀룰로오스, 알루미늄 메타히드록시드, 벤토나이트, 아가 또는 트라칸트 등이 이용될 수 있다.When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.
본 발명의 제형이 계면-활성제 함유 클렌징인 경우에는 담체 성분으로서 지방족 알코올 설페이트, 지방족 알코올 에테르설페이트, 설포숙신산 모노에스테르, 이세티오네이트, 이미다졸리늄 유도체, 메틸타우레이트, 사르코시네이트, 지방산 아미드 에테르 설페이트, 알킬아미도베타인, 지방족 알코올, 지방산 글리세리드, 지방산 디에탄올아미드, 식물성 유, 리놀린 유도체 또는 에톡실화 글리세롤 지방산 에스테르 등이 이용될 수 있다.When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component is selected from aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolenic derivatives or ethoxylated glycerol fatty acid esters.
본 발명의 제형은 형광물질, 살진균제, 굴수성 유발물질, 보습체, 방향제, 방향제 담체, 단백질, 용해화제, 당유도체, 일광차단제, 비타민, 식물 추출물 등을 포함하는 부형제를 추가로 함유할 수 있다.The formulation of the present invention may further contain an excipient including a fluorescent substance, a fungicide, a hygroscopic substance, a humidifier, a fragrance, a fragrance carrier, a protein, a solubilizing agent, a sugar derivative, a sunscreen, a vitamin, .
본 발명에 따른 식품 조성물은 식품첨가물로 사용될 수 있고, 식품첨가물로 사용될 경우, 상기 조성물을 그대로 첨가하거나 다른 식품 또는 식품 성분과 함께 사용될 수 있고, 통상적인 방법에 따라 적절하게 사용될 수 있다. 유효 성분의 혼합양은 그의 사용 목적(예방, 건강 또는 치료적 처치)에 따라 적합하게 결정될 수 있다. 일반적으로, 식품 또는 음료의 제조시에 본 발명의 조성물은 원료에 대하여 15 중량부 이하, 바람직하게는 10 중량부 이하의 양으로 첨가된다. 그러나, 건강 및 위생을 목적으로 하거나 또는 건강 조절을 목적으로 하는 장기간의 섭취의 경우에는 상기 양은 상기 범위 이하일 수 있으며, 안전성 면에서 아무런 문제가 없기 때문에 유효성분은 상기 범위 이상의 양으로도 사용될 수 있다.The food composition according to the present invention can be used as a food additive. When used as a food additive, the composition can be added as it is, or can be used together with other food or food ingredients, and can be suitably used according to a conventional method. The amount of the active ingredient to be mixed can be suitably determined according to its intended use (prevention, health or therapeutic treatment). Generally, the composition of the present invention is added in an amount of not more than 15 parts by weight, preferably not more than 10 parts by weight, based on the raw material, when the food or beverage is produced. However, in the case of long-term consumption intended for health and hygiene purposes or for health control purposes, the amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount exceeding the above range .
상기 식품의 종류에는 특별한 제한은 없다. 상기 물질을 첨가할 수 있는 식품의 예로는 육류, 소시지, 빵, 초콜릿, 캔디류, 스낵류, 과자류, 피자, 라면, 기타 면류, 껌류, 아이스크림류를 포함한 낙농제품, 각종 스프, 음료수, 차, 드링크제, 알콜 음료 및 비타민 복합제 등이 있으며, 통상적인 의미에서의 식품을 모두 포함한다.There is no particular limitation on the kind of the food. Examples of foods to which the above substances can be added include dairy products including meats, sausages, breads, chocolates, candies, snacks, confectionery, pizza, ramen noodles, gums, ice cream, soups, drinks, tea, Alcoholic beverages, and vitamin complexes, and includes foods in a conventional sense.
본 발명의 음료 조성물은 통상의 음료와 같이 여러 가지 향미제 또는 천연 탄수화물 등을 추가 성분으로서 함유할 수 있다. 상술한 천연 탄수화물은 포도당, 과당과 같은 모노사카라이드, 말토스, 슈크로스와 같은 디사카라이드, 및 덱스트린, 사이클로덱스트린과 같은 폴리사카라이드, 자일리톨, 소르비톨, 에리트리톨 등의 당알콜이다. 감미제로서는 타우마틴, 스테비아 추출물과 같은 천연 감미제나, 사카린, 아스파르탐과 같은 합성 감미제 등을 사용할 수 있다.The beverage composition of the present invention may contain various flavors or natural carbohydrates as an additional ingredient such as ordinary beverages. Such natural carbohydrates are monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, and polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol and erythritol. Examples of sweeteners include natural sweeteners such as tau martin and stevia extract, synthetic sweeteners such as saccharin and aspartame, and the like.
상기 외에 본 발명의 조성물은 여러가지 영양제, 비타민, 전해질, 풍미제, 착색제, 펙트산 및 그의 염, 알긴산 및 그의 염, 유기산, 보호성 콜로이드 증점제, pH 조절제, 안정화제, 방부제, 글리세린, 알콜, 탄산음료에 사용되는 탄산화제 등을 함유할 수 있다. 그 밖에 본 발명의 조성물은 천연 과일 주스, 과일 주스 음료 및 야채 음료의 제조를 위한 과육을 함유할 수 있다. 이러한 성분은 독립적으로 또는 혼합하여 사용할 수 있다. 이러한 첨가제의 비율은 크게 중요하진 않지만 본 발명의 조성물 100 중량부당 0.01~0.1 중량부의 범위에서 선택되는 것이 일반적이나, 이에 제한되는 것은 아니다.In addition to the above, the composition of the present invention may further contain various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and salts thereof, alginic acid and its salts, organic acids, protective colloid thickeners, pH adjusters, stabilizers, preservatives, glycerin, A carbonating agent used in beverages, and the like. In addition, the composition of the present invention may contain flesh for the production of natural fruit juices, fruit juice drinks and vegetable drinks. These components may be used independently or in combination. Although the ratio of these additives is not critical, it is generally selected from the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the composition of the present invention, but the present invention is not limited thereto.
본 발명에 의한 약학 조성물의 제형에 있어서, 유효성분의 함유량은 제형에 따라 광범위하게 변할 수 있으며, 통상적인 방법에 따른다.In the formulation of the pharmaceutical composition according to the present invention, the content of the active ingredient may vary widely depending on the formulations, and is generally according to a conventional method.
본 발명에 의한 약학 조성물은, 유효성분에 악영향을 미치지 않는 한, 필요에 따라 의약에 사용되는 각종 보조제, 예컨대 담체나 기타 첨가제, 예컨대 안정제, 완화제, 유화제 등을 첨가하여 제제화될 수 있다.The pharmaceutical composition according to the present invention may be formulated by adding various adjuvants such as carriers and other additives such as stabilizers, emollients, emulsifiers and the like, which are used in medicines, if necessary, so long as they do not adversely affect the active ingredients.
또한, 본 발명에 따른 조성물은 비경구 또는 경구 등으로 투여할 수 있다. 비경구 투여 루트로는 경피 투여가 바람직하며, 그 중에서도 국소도포가 가장 바람직하다. 예를 들어, 반창고 형태로 제조하여 붙일 수 있으나, 이에 제한되지 않는다. 제형으로는, 연고, 크림, 주사제, 산제, 과립제, 정제 등을 비롯하여 약학적 제제에 적합한 어떠한 제형으로도 할 수 있다.In addition, the composition according to the present invention can be administered parenterally or orally. As the route of parenteral administration, transdermal administration is preferable, and local application is most preferable. For example, it may be manufactured in the form of a bandage, but is not limited thereto. The formulations may be any formulations suitable for pharmaceutical preparations including ointments, creams, injections, powders, granules, tablets, and the like.
본 발명에 의한 약제학적 조성물의 바람직한 투여량은 0.001 내지 1000 ㎎/kg·day일 수 있으나, 이에 제한되지 않는다. 또한, 본 발명에 의한 조성물은 단독으로 투여되거나 다른 약제와 동등하게 또는 다른 약제를 보조하기 위해 함께 투여될 수 있다. 또한, 각 제형의 조성물에 있어서, 상기한 필수 성분인 조성물 이외의 다른 성분들은 기타 외용제의 제형 또는 사용목적 등에 따라 통상의 기술자가 선정할 수 있으며, 이 경우 다른 원료와 동시에 적용할 경우 상승 효과가 일어날 수 있다.The preferred dosage of the pharmaceutical composition according to the present invention may be 0.001 to 1000 mg / kg · day, but is not limited thereto. In addition, the composition according to the present invention may be administered alone, or may be administered together with other medicines in order to aid in the same or another medicament. In addition, in the composition of each formulation, components other than the above-mentioned essential component, other than the composition, can be selected by a typical technician according to the formulation or purpose of use of the other external preparation. In this case, Can happen.
이하, 실시예를 통하여 본 발명을 더욱 상세히 설명하기로 한다. 이들 실시예는 본 발명을 보다 구체적으로 설명하기 위한 것으로서, 본 발명의 범위가 이들 실시예에 한정되는 것은 아니다.Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are for further illustrating the present invention, and the scope of the present invention is not limited to these examples.
실시예Example 1. 재료 및 방법 1. Materials and Methods
1.1. 실험재료 및 발아1.1. Materials and germination
실험에 사용된 보리는 농촌진흥청 국립식량과학원으로부터 제공받았으며, 보리 품종은 다한, 흑누리, 흑광을 사용하였다. 발아는 보리 500 g을 선별하였고, 수세한 뒤, 18℃에서 1일간 증류수에 침지시킨 다음 발아기(HB-302M, Hanbaek, Bucheon, Korea)로 발아시켰다. 발아 시에는 18℃를 유지시켰으며, 1일 1회 세척 및 물주기를 수행하였다. 발아기간은 0일(침지)에서 6일로 하였으며, 발아시키지 않은 보리를 대조구로 하였다. 발아된 보리는 50℃의 열풍건조기(WFO-459PD, EYELA, Tokyo, Japan)에서 2일간 건조시킨 다음 분쇄기(Micro hammer cutter mill type-3, Culatti AG, Zurich, Swiss)를 사용하여 80 mesh 이하로 분쇄하고, 냉동보관하면서 시료로 사용하였다.The barley used in the experiment was supplied by National Institute of Food Science and Technology, RDA. In germination, 500 g of barley was selected, washed, and then immersed in distilled water for 1 day at 18 ° C and then germinated in a germination machine (HB-302M, Hanbaek, Bucheon, Korea). During germination, the temperature was maintained at 18 ° C and washing and watering were performed once a day. Germination period was from 0 day (immersion) to 6 days, and non - germinated barley was used as a control. The germinated barley was dried in a hot air drier (WFO-459PD, EYELA, Tokyo, Japan) at 50 ° C for 2 days and then pulverized using a grinder (Micro hammer cutter mill type-3, Culatti AG, Zurich, Swiss) Crushed, and stored frozen and used as a sample.
1.2. 추출물 제조1.2. Extract preparation
시료 중에 함유된 유용성분을 추출하기 위해 메탄올 추출물을 제조하였다. 메탄올 추출물은 각각 시료 중량 대비 10배량의 80% 메탄올(v/v)을 첨가하여 1시간 동안 3회 반복하여 초음파추출하고, 이 추출물을 감압여과한 후 농축하여 동결건조(Freeze dryer, FD5508, Ilshin Lab Co., Ltd., Dongducheon, Korea)한 다음 -20℃에서 보관하면서 시료로 사용하였다.A methanol extract was prepared to extract useful components contained in the sample. The methanol extracts were subjected to ultrasonic extraction by repeating three times for 1 hour by adding 10 times of 80% methanol (v / v) to the weight of each sample. The extract was filtered under reduced pressure, concentrated and freeze-dried (FD5508, Ilshin Lab Co., Ltd., Dongducheon, Korea) and stored at -20 ° C for use as a sample.
1.3. 용매 1.3. menstruum 분획물Fraction 제조 Produce
제조된 각각의 추출물은 증류수에 용해한 후 헥산(hexane), 클로로폼(chloroform), 에틸아세테이트(ethyl acetate), 부탄올(butanol) 및 물(water) 순으로 순차 분획하였으며, 이들 분획물들은 질소 농축, 감압 농축 및 동결 건조하여 분말형태로 제조하여 실험에 사용하였다.Each of the extracts was dissolved in distilled water and then sequentially fractionated in the order of hexane, chloroform, ethyl acetate, butanol and water. These fractions were concentrated in nitrogen, Concentrated and lyophilized to prepare powder and used in the experiment.
1.4. 자외선 조사 및 세포생존율 평가1.4. UV irradiation and evaluation of cell viability
HS68 세포를 96-well plate에 1.0 × 104 cells/well씩 분주하고 24 시간 배양한 후, FBS를 포함하지 않는 배지 및 추출물로 24 시간 처리하였다. 처리한 추출물의 농도는 100μg/mL 이었으며, 24 시간 후에 배지를 제거하고 PBS를 이용하여 세포를 헹구어 준 다음, 100 μL의 PBS 를 well에 분주 후 UVB를 30 mJ/cm2 (30s 동안)로 조사하였다. UVB 조사 시 UVB lamp(Sankyo denki Lamps, GL20SE, Marine, Japan)를 사용하였고, 광원의 강도는 UV LIGHT METER(LT Lutron, UV-340A, Taiwan)를 사용하여 측정하였다. UVB 조사 후, 남아 있는 PBS를 제거한 후, 새로운 PBS로 한 번 더 세포를 헹구어 준 뒤, FBS를 포함하지 않는 배지 및 추출물을 100μg/mL의 농도로 24 시간 처리하였다. 이후에 MTT solution(1 mg/mL)을 넣고 37 ℃, 5 % CO2 배양기에서 배양하였으며, 4 시간 후에 MTT solution을 제거하고 생성된 formazan crystal을 DMSO에 녹여 microplate reader(Bio Tek, Inc., Winooski, VT, USA)로 550 nm에서 흡광도를 측정하였다.HS68 cells were seeded at a density of 1.0 × 10 4 cells / well on a 96-well plate, cultured for 24 hours, and treated with media and extracts containing no FBS for 24 hours. The concentration of the treated extract was 100 μg / mL. After 24 hours, the medium was removed, and the cells were rinsed with PBS. 100 μL of PBS was dispensed into wells, and UVB was irradiated at 30 mJ / cm 2 . UVB lamp (Sankyo Denki Lamps, GL20SE, Marine, Japan) was used for the UVB irradiation, and the intensity of the light source was measured using a UV light meter (LT Lutron, UV-340A, Taiwan). After the UVB irradiation, the remaining PBS was removed, and the cells were rinsed once more with fresh PBS. Then, the FBS-free medium and the extract were treated at a concentration of 100 μg / mL for 24 hours. After 4 hours, the MTT solution was removed, and the formazan crystal was dissolved in DMSO. The microplate reader (BioTek, Inc., Winooski, Japan) was added to each well and incubated at 37 ° C in a 5% CO 2 incubator , VT, USA) at 550 nm.
실시예Example 2. 보리 발아 추출물의 2. Extract of barley germination UVB로With UVB 유도한 Induced 피부광노화의Of skin photoaging 보호효과 Protection effect
2.1. 추출물의 세포독성 평가2.1. Evaluation of cytotoxicity of extracts
다한, 흑누리, 흑광, 보석찰의 원곡 및 발아(0일 내지 6일) 보리 추출물에 대한 세포독성 평가를 실시하였다. 그 결과, 상기 추출물 모두 100 μg/mL의 농도에서 세포독성이 나타나지 않음을 확인하였다(도 1 내지 4).To evaluate the cytotoxicity of barley extracts of black, white, black light, gemstones and germination (0 to 6 days). As a result, it was confirmed that the extracts did not show cytotoxicity at a concentration of 100 μg / mL (FIGS. 1 to 4).
2.2. 추출물의 2.2. Extract UVB로With UVB 유도한 피부 Derived skin 광노화Photoaging 보호 효과(HS68 cell) Protection effect (HS68 cell)
보리 추출물에 대하여 UVB로 유도한 피부 광노화 보호 효과를 실험하였다. 품종에 따른 원곡 및 발아 일수별 피부 광노화 보호 효과를 살펴 보면, 다한의 경우 발아 3일, 4일 차에 높은 피부 보호 효과를 보였으며, 흑누리의 경우 발아 5일, 6일 차에, 흑광의 경우 발아 6일 차, 보석찰의 경우 발아 2일과 발아 6일차에 높은 피부 보호 효과를 보였다(도 5 내지 8). The UVB - induced skin photoprotection effect of barley extracts was investigated. On the other hand, the skin protection effect on the skin of 3 days and 4 days of germination was higher than that of the other skin. On the 5th day and 6th day of germination, 6 days for germination, and 2 days for germination and 6 days for germination in the case of gemstones (Figs. 5 to 8).
따라서, 보리의 원곡추출물에 비해 보리의 발아 시 광노화에 의한 피부 보호 효과가 증가됨을 확인하였다. Therefore, it was confirmed that the skin protection effect by photoaging during germination of barley is increased compared to the original extract of barley.
또한, 앞서 품종별 높은 피부 보호 효과를 보인 다한 발아 3일, 4일, 흑누리 발아 5일, 6일, 흑광 5일, 6일, 보석찰 2일, 6일차에 대하여 보호 효과를 비교하는 실험을 진행하여 가장 높은 효과를 보인 시료를 선정하고자 하였다. 그 결과, 다한 3일차 발아에서 가장 높은 광노화 보호 효과가 나타남을 확인하였다(도 9).In addition, the experiment to compare the protective effect against the germination 3 days, 4 days, 5 days, 6 days, 6 days, 6 days, 6 days, To select the most effective sample. As a result, it was confirmed that the highest photoprotection effect was observed in the third day germination (Fig. 9).
실시예Example 3. 용매 3. Solvent 분획물의Fraction UVB로With UVB 유도한 Induced 피부광노화의Of skin photoaging 보호효과 Protection effect
3.1. 용매 3.1. menstruum 분획물의Fraction 세포독성 평가 Cytotoxicity Assessment
상기 실시예 2의 결과에서 가장 우수한 피부 광노화 보호효과를 보인 다한 발아 3일차 추출물의 용매 분획물에 대하여 세포독성을 평가하였다. 그 결과, 헥산(hexane), 클로로폼(chloroform), 에틸아세테이트(ethyl acetate), 부탄올(butanol) 및 물(water)의 5가지 분획물 모두 100 μg/mL의 농도에서 독성을 나타나지 않았으며, 클로로폼 분획물에서는 세포 증식이 관찰되었다(도 10). The cytotoxicity of the solvent fraction of the germinated 3rd day germ extract, which exhibited the best skin photoaging protection effect, was evaluated in the results of Example 2 above. As a result, the five fractions of hexane, chloroform, ethyl acetate, butanol and water showed no toxicity at a concentration of 100 μg / mL, Cell proliferation was observed in the fraction (Fig. 10).
3.2. 용매 3.2. menstruum 분획물의Fraction UVB로With UVB 유도한 피부 Derived skin 광노화Photoaging 보호 효과(HS68 cell) Protection effect (HS68 cell)
다한 발아 3일차 추출물에 대하여 헥산(hexane), 클로로폼(chloroform), 에틸아세테이트(ethyl acetate), 부탄올(butanol) 및 물(water) 순으로 순차적 용매 분획을 실시하고, 그 용매 분획물에 대하여 UVB로 유도한 피부 광노화 보호 효과를 조사하였다. 그 결과, 용매 분획시 헥산(Hexane), 부탄올(Butanol), 물(Water) 분획물은 다한 발아 3일차 추출물보다 효과가 낮아지는 경향을 보였으며, 에틸아세테이트(Ethyl acetate) 분획물은 다한 발아 3일차 추출물과 유사한 효과를 보였다. 가장 큰 피부 광노화 보호효과를 보인 분획물은 클로로폼(Chloroform) 분획물인 것으로 나타났다(도 11). Sequential solvent fractions of the germinated third day extract were sequentially applied in the order of hexane, chloroform, ethyl acetate, butanol and water. The solvent fractions were subjected to UVB treatment Induced skin photoprotection. As a result, the hexane (Hexane), butanol, and water fractions in the solvent fraction tended to be less effective than the germinated third day extracts. Ethyl acetate fraction was obtained from three different germination extracts . The fraction showing the greatest skin photoprotection effect was a chloroform fraction (Fig. 11).
따라서, 발아 보리 추출물 및 분획물은 발아 전 원곡보리에 비해 UVB에 의한 피부 광노화 효과가 증가됨을 확인하였다. Therefore, the germinated barley extracts and fractions were found to have an increased skin photoaging effect due to UVB compared with the raw barley before germination.
Claims (9)
상기 조성물은 자외선에 의한 피부세포 손상을 억제하는 것을 특징으로 하는 조성물. The method according to claim 1,
Wherein said composition inhibits skin cell damage by ultraviolet light.
상기 추출물 또는 분획물은 화장료 조성물 총 중량에 대하여 0.0001 내지 20 중량부인 것을 특징으로 하는 조성물.The method according to claim 1,
Wherein the extract or fraction is 0.0001 to 20 parts by weight based on the total weight of the cosmetic composition.
상기 조성물은 피부외용연고, 크림, 유연화장수, 영양화장수, 팩, 에센스, 젤, 스킨로션, 스킨소프너, 스킨토너, 아스트린젠트, 로션, 밀크 로션, 모이스처 로션, 영양로션, 마사지 크림, 영양크림, 모이스처 크림, 핸드 크림, 파운데이션, 영양에센스, 선스크린, 비누, 클렌징폼, 클렌징로션, 클렌징크림, 바디 로션 및 바디 클렌저로 이루어진 그룹에서 선택되는 어느 하나의 제형으로 제조된 것을 특징으로 하는 조성물.The method according to claim 1,
The composition can be used as an external ointment for skin, a cream, a softening agent, a nutritional lotion, a pack, an essence, a gel, a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, Wherein the composition is selected from the group consisting of a cream, a hand cream, a foundation, a nutrition essence, a sunscreen, a soap, a cleansing foam, a cleansing lotion, a cleansing cream, a body lotion and a body cleanser.
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070129428A1 (en) * | 2003-12-18 | 2007-06-07 | Myriam Richelle | Composition for improving skin, hair and coat health containing flavanones |
| KR20110085758A (en) * | 2010-01-21 | 2011-07-27 | 대한민국(관리부서:농촌진흥청장) | Extracts from young barley leaves including polyphenol compounds and their manufacturing method |
| KR20120048321A (en) * | 2010-11-05 | 2012-05-15 | 경희대학교 산학협력단 | Cosmetic composition for skin whitening comprising a self-lysis product of germinated barley |
| KR20140015668A (en) * | 2012-06-29 | 2014-02-07 | 애경산업(주) | Cosmetic composition for skin improvement containing bud extract |
| KR20140095122A (en) * | 2013-01-18 | 2014-08-01 | 가천대학교 산학협력단 | A composition for inhibiting degradation of collagen or promoting synthesis of collagen comprising barley extract or eriodictyol |
| KR20160116523A (en) * | 2015-03-30 | 2016-10-10 | 대한민국(농촌진흥청장) | Cosmetic composition comprising extract from young barley leaves having whitening and anti-wrinkle activity |
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Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070129428A1 (en) * | 2003-12-18 | 2007-06-07 | Myriam Richelle | Composition for improving skin, hair and coat health containing flavanones |
| KR20110085758A (en) * | 2010-01-21 | 2011-07-27 | 대한민국(관리부서:농촌진흥청장) | Extracts from young barley leaves including polyphenol compounds and their manufacturing method |
| KR20120048321A (en) * | 2010-11-05 | 2012-05-15 | 경희대학교 산학협력단 | Cosmetic composition for skin whitening comprising a self-lysis product of germinated barley |
| KR20140015668A (en) * | 2012-06-29 | 2014-02-07 | 애경산업(주) | Cosmetic composition for skin improvement containing bud extract |
| KR20140095122A (en) * | 2013-01-18 | 2014-08-01 | 가천대학교 산학협력단 | A composition for inhibiting degradation of collagen or promoting synthesis of collagen comprising barley extract or eriodictyol |
| KR20160116523A (en) * | 2015-03-30 | 2016-10-10 | 대한민국(농촌진흥청장) | Cosmetic composition comprising extract from young barley leaves having whitening and anti-wrinkle activity |
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