KR101853788B1

KR101853788B1 - Adsorption method of whey protein using pine bark and pine bark polyphenols

Info

Publication number: KR101853788B1
Application number: KR1020160024398A
Authority: KR
Inventors: 문성필
Original assignee: 전북대학교산학협력단
Priority date: 2016-02-29
Filing date: 2016-02-29
Publication date: 2018-05-02
Also published as: KR20170101615A

Abstract

The present invention relates to a process for the production of a bark-whey protein complex or a bark-whey protein complex, wherein the proanthocyanidins (PAs), which are the main components of the pine bark and pine bark, are selectively adsorbed with the whey protein and the adsorption temperature is 0-50 ° C. and the adsorption time is 2-12 hours. PAs-whey protein complexes. The complex thus obtained can be used for functional protein foods as well as functional foods for livestock.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for adsorbing whey proteins using pine bark and pine bark polyphenols,

The present invention establishes a condition capable of adsorbing and recovering most of whey protein in a short time by treating pine bark or pine bark polyphenol to whey. In addition, proanthocyanidins (PAs), a polyphenol which is the main component of the pine bark, are the main ingredients for the adsorption of whey proteins. We have established the conditions for the combination of pine bark PAs and whey protein, .

Polyphenols such as tannins are compounds that are widely found in plants. Among these polyphenols, proanthocyanidins (PAs, see FIG. 1), which is a kind of condensed tannins, are widely distributed in leaves, fruits, bark and roots of various plants and are used for foods such as beer, . Condensed tannins are known to form complexes with high affinity for proteins, especially proline-rich proteins (Siebert, KJ et al., J. Agri. & Food Chem., 44 ), Siebert, KJ, J. Agri. &Amp; Food Chem., 47 (2); 353 (1999), Poncet-Legrand, C. et al., Food Hydrocolloids, 20 (5); 687 (2006)). Study of the sphere, etc. (Ku, CS et al, Chemosphere , 67 (8);. 1618 ( according to 2007), proline-rich proteins, one of the first type of collagen is radiah other pine (Pinus radiata ) bark, respectively. That is, the adsorption of PAs to type 1 collagen was optimized by pH, contact time and temperature. As a result, various concentration of PAs solution reached adsorption equilibrium with collagen at 285 minutes and showed optimum adsorption state at 37 ℃ and 50 ℃. Collagen also absorbed about 80-85% of PAs by weight. The following formula (1) is a chemical formula showing the structure of proanthocyanidin (PAs) of pine bark.

These protein - affinity PAs are the major component of the bark of Rhodia pine. At present, the import volume of Radiata pine ash accounts for about 50% of the total wood imports, and most of them are imported from New Zealand. Radiata pine wood is widely used for building construction, packaging, fiberboard and pulp production. The bark is derived as a by-product in the related wood industry, and it is mainly used as a heat source or discarded. However, as described above, pine bark is a major source of PAs, and bark extracts in Europe are used as dietary supplements for the prevention and treatment of skin aging and cardiovascular diseases (Rohdewald, P., Intl. J. Clinical Pharmacology & Therapeutics , 40 (4); 158 (2002)). Lipids, 37 (10), 931 (2002), Liu, X. et al., Life Sciences, 74 (7); 855 (2004)). Therefore, it is necessary to apply the bark of Radiata pine, which is currently being mass - disused in Korea, to the production of high value added materials.

On the other hand, whey is a by-product of cheese production, and 90% of milk is released in the form of whey. About 94% of whey is water, and most of the remaining solids are lactose. Lactose The next highest solids content was protein (0.8%, see Table 1), with β-lactoglobulin, FV, Cheese and Fermented Milk Foods, Edwards Brothers, Inc., Ann Arbor, These whey proteins are water-soluble proteins and are relatively spherical proteins with a small molecular weight, and are shown in Table 2 (Whitney, RM et al., J. Dairy sci., 59 Reiter, B., Bulletin-Federation Internationale de Laiterie (Belgium), < RTI ID = 0.0 > Kitchen, B (1985). Developments in Dairy Chemistry 3, Springer Netherlands, p. 239 (1985); Wang, CS, Comparative Biochem. & Physiology Part B: Comparative Biochem., 78 (3), 575 At present, a large-scale dairy factory concentrates these whey products to produce related products, but in a small-scale cheese factory, As shown in Table 1. Table 1 shows the general composition of whey solution and whey powder, and Table 1 shows the general composition of whey solution and whey powder, Table 2 below shows the physicochemical properties of whey proteins.

The present technology was designed to examine the binding properties of wheat protein to radish pine bark and bark PAs, taking into account the affinity of bark polyphenol and protein.

Whey processing after cheese production is a big problem in small-scale cheese factories as well as large-scale cheese factories. The reason is that most of the whey is water, which is expensive to make whey powder by concentrating it. Also, the price of imported whey powder is lower than that of domestic ones, which makes it less competitive. Therefore, the whey of many small and medium cheese companies is treated as wastewater and discharged or used for some animal feeds. Therefore, it is very necessary to develop a method for economically separating specific components of whey. Currently, methods for separating and purifying lactose or expensive protein present in these whey through membrane separation have been developed, but the facility investment cost is very high and economical.

SUMMARY OF THE INVENTION The present invention has been made to solve the above problems, and it is an object of the present invention to provide a process for producing a whey protein by adsorbing and precipitating a whey protein in a short time by treating a pine bark powder or a pine bark polyphenol containing polyphenol as a main component, It shows how you can separate. That is, the present invention provides a method for easily and quickly separating whey protein from high-priced whey protein by using polyphenol produced in vine or pine bark.

It is another object of the present invention to provide a feed additive and a functional food containing the isolated whey protein.

The present invention provides a method for adsorbing whey protein using polyphenols in order to solve the first technical problem described above.

According to an embodiment of the present invention, the method for adsorbing whey protein comprises the steps of: preparing a polyphenol powder prepared from pine bark powder or pine bark having a predetermined particle size; Treating the pine bark powder or polyphenol powder with whey to make a pine bark whey protein composition or a polyphenol whey protein composition; And isolating the pine bark whey protein or polyphenol whey protein composition.

According to another embodiment of the present invention, the method of adsorbing the whey protein may be performed at a temperature of 30 to 50 DEG C for 5 to 60 minutes.

According to another embodiment of the present invention, the pine bark composition may be at least one selected from the group consisting of polyphenols derived from pine bark, catechin, resveratrol, quercitin, or isoflavone.

According to another embodiment of the present invention, the polyphenols derived from the pine bark may be proanthocyanidins (PAs).

The present invention also provides a feed additive comprising a pine bark whey protein composition or a polyphenol whey protein composition prepared by the above method to solve the second technical problem.

According to another embodiment of the present invention, there is provided a functional food comprising a pine bark whey protein composition or a polyphenol whey protein composition prepared by the above method.

It is possible to treat pine bark by simply treating the pine bark without using the expensive method such as separation of the expensive whey protein and lactose of the present invention and separating the lactose by the addition of bark polyphenol, Is a method capable of selectively separating whey proteins selectively from lactose.

The pine bark-whey protein complex obtained by the present invention and the polyphenol whey protein complex containing pine bark -PAs as a main component can be used as a high-feed feed additive for ruminants and various functional foods or food additives.

1 is a graph showing an HPLC chromatogram of a sample prepared under various adsorption conditions according to an embodiment of the present invention.
FIG. 2 is a graph showing another rate of weight increase in adsorption time according to another embodiment of the present invention. FIG.
FIG. 3 is a graph showing a change in weight increase rate with time of adsorption under various temperature conditions according to another embodiment of the present invention. FIG.
FIG. 4 is a graph showing the change of adsorption rate of each whey protein according to the adsorption time according to another embodiment of the present invention.

Hereinafter, the present invention will be described in detail.

It is noted that the technical terms used in the present invention are used only to describe specific embodiments and are not intended to limit the present invention. In addition, the technical terms used in the present invention should be construed in a sense generally understood by a person having ordinary skill in the art to which the present invention belongs, unless otherwise defined in the present invention, Should not be construed to mean, or be interpreted in an excessively reduced sense. In addition, when a technical term used in the present invention is an erroneous technical term that does not accurately express the concept of the present invention, it should be understood that technical terms that can be understood by a person skilled in the art are replaced with a technical term. And should not be construed as being excessively reduced in meaning, and the singular expressions used in the present invention include plural expressions unless the context clearly dictates otherwise. &Lt; RTI ID = 0.0 > And it is to be understood that the present invention may be embodied with additional constituent elements or steps. In the following description of the present invention, a detailed description of related arts will be omitted. It is omitted.

Disclosure of Invention Technical Problem [8] The present invention has been devised to solve the above-mentioned problems, and the present invention provides a method of adsorbing whey protein using a pine bark composition to solve the above-mentioned first technical problem.

According to another embodiment of the present invention, the pine bark composition may be a polyphenol derived from pine bark.

Hereinafter, the constitution and effects of the present invention will be described in more detail with reference to the accompanying drawings, examples, comparative examples and experimental examples. These embodiments are only for illustrating the present invention, and the scope of the present invention is not limited by these embodiments.

The present invention relates to a process for producing whey protein by pulverizing and pulverizing pine bark, treating pine bark with whey, and adsorbing whey protein in whey to easily isolate whey protein by pine bark-whey protein complexation And may include a step.

Also disclosed is a process for producing a polyphenol-whey protein complex comprising a step of producing a polyphenol containing as a main component PAs as a main component of pine bark, a step of treating the produced bran polyphenol to whey, and a step of adsorbing whey protein in whey The whey protein can be easily separated from the whey protein.

In the method for producing pine bark or bark polyphenols according to the present invention, the pine bark may be, but not limited to, a pine bark of Radida pine, a reed bark, a black pine bark, and a pine bark of Rigida pine.

In addition, the pine bark of the present invention can use a powder having a particle size of 40 to 325 mesh, preferably a powder having a pine bark particle size of 80 to 200 mesh, A powder having a pine bark particle size of 100 to 150 mesh may be used.

In the method for producing polyphenols from the pine bark of the present invention, the pine bark may be further subjected to one or more methods selected from hot water extraction, organic solvent extraction, ultrasonic treatment, low frequency treatment, electric field treatment and magnetic field treatment. It is not.

In the method for producing polyphenol of pine bark of the present invention, the step of producing the polyphenols containing the PAs as a main component comprises the steps of mixing the pine bark and various extracting solvents in a weight ratio of 1: 2 to 1: 100, And extracting the pine bark powder at a temperature of 1 to 100 ° C, preferably a room temperature to 100 ° C, more preferably 80 to 100 ° C for 5 to 60 minutes. In the method for producing polyphenols of the present invention, the above " room temperature " means 20 to 25 ° C.

In the method for producing polyphenols according to the present invention, an extract containing polyphenols can be obtained from the pine bark by preparing a polyphenol containing the PAs as a main component.

In the present invention, the treating temperature of the pine bark or bark polyphenol may be 0 ° C to 80 ° C.

In the present invention, the treatment time of pine bark or bark polyphenol may be from 5 minutes to 12 hours, preferably from 5 minutes to 60 minutes.

< Experimental Example 1> Pine bark Of whey protein absorption

1.1 Experimental Material

The Pinus radiata bark, which was supplied from HanYoung Kim, Gunsan City, was crushed and passed through a 1 mm sieve, which was used as a sample for whey protein adsorption. The whey was provided by the Dishil Cheese Science Research Institute, and it was centrifuged (10 ° C, 652 x g ) twice for 10 minutes, and the fat layer was removed and lyophilized. This frozen dry bulk powder was used as a whey powder sample.

1.2 Adsorption experiment

1 g, 1.25 g, 2.5 g, and 5 g of the above-described pine bark powder were respectively used for the adsorption test with whey. 3.175 g of the lyophilized whey powder was completely dissolved by adding distilled ion exchange water to a ratio of 10 based on the bark powder weight (for example, in case of 1 g bark, 3.175 g of whey powder was added to 10 g of distilled ion-exchanged water) . The whey was mixed with the bark powder and shaken for 30 minutes at a water bath for a designated time. The mixture was filtered under reduced pressure through a glass filter and combined with the residue wash water for subsequent analysis. As a control experiment, 5 g of pine bark powder was mixed with distilled ion-exchanged water (50 ml) instead of whey and treated under the same conditions as above. The filtrate was allowed to stand in a refrigerator at 4 ° C for one night. After centrifugation, the precipitate and supernatant were freeze-dried for some filtrates in which precipitation was formed. The filtration residue was dried in a 105 ° C dryer for 12 hours and the weight gain was calculated.

1.3 Instrument Analysis

In the HPLC analysis, 1 ml of the reduced whey prepared at the same concentration as that used for bark adsorption was diluted with 1.5 ml of distilled ion-exchanged water. In the case of the filtrate after the adsorption of bark and whey protein, 1 ml was taken and 1 ml of distilled ion-exchanged water was added and diluted 2-fold. All dilutions were filtered with a 0.45 ㎛ PTFE syringe filter and then loaded on a Shimadzu LC-20AD (Japan) using Jupiter C4 (250 × 4.6 mm, 5 μ, 300 Å, Phenomenex, USA) Respectively. The analysis tide was as shown in Table 3 below.

The elemental analysis The lyophilized whey powder, filtrate freeze-dried powder (including supernatant, precipitate) and dried residue were pulverized to fine powder, which was vacuum-dried under P ₂ O _{5 and} analyzed by elemental analysis (Elemento Analysensysteme GmbH, Germany).

1.4 Results

When a certain amount of whey was treated with respect to the weight of various pine bark, there was a weight loss of 15.6% in the case of bark alone (control) as shown in Table 4 below, and the weight loss was due to the elution of water extract in pine bark . When whey was used instead of water for the same bark amount, the weight reduction rate was 7%, which was about 9% lower than that of bark alone. This fact suggests that some of the components of whey not only delayed the extraction of bark extracts, but some of them were due to adsorption to bark. As the bark amount decreased, the weight increase rate of the residue after adsorption increased remarkably. In the case of 2.5 g of bark, the residue weight after adsorption increased by 4.58%. However, in the case of bark 1.25 g, the residue weight increased by 25.2% even though the bark amount was small. In general, the whey protein is about 13%, and when the whey protein is 100% adsorbed to the bark, the residue weight increases theoretically by 0.42 g. Assuming that the bark weight gain in Table 4 is only due to the adsorption of whey protein, it means that about 76% of the whey proteins were adsorbed at 1.25 g of bark. When the polyphenol content and the whey protein ratio are 3: 2 (Ku, CS et al., J. Wood Science 53 (6), 524 Bark 1.25 g) was confirmed to be the optimum condition for adsorption of 75% or more whey protein. It was also expected that the whey protein adsorbed rapidly to the bark polyphenol within 1 hour, when the whey protein was adsorbed to 1.25 g of bark for 1 hour and 5 hours, respectively.

On the other hand, the whey protein in the filtrate after the adsorption was analyzed by HPLC and the results are shown in Fig. In the case of whey, peaks of α-lactalbumin, β-lactoglobulin A, and β-lactoglobulin B, which are the main proteins of whey, are well separated as shown in FIG. 1 a (Buffoni, JN et al., Food Chemistry, 127 4), 1515 (2011)). However, when the whey was added with bark (5 g, 2.5 g, 1.25 g) and shaken for 5 hours, most of the whey protein-derived peaks disappeared as shown in Fig. 1, c, d and e. The PAs-related peaks appear at a retention time (RT) of 3-5 minutes as shown in Figure 1 (b), overlapping with a small amount of peaks of other whey-derived substances, and an analysis of the presence or absence of adsorption of whey- Was impossible.

Elemental analysis was performed on the residue obtained after the adsorption experiment and whey and the bark obtained only by treatment with water only under the same conditions. The results are shown in Table 5 below.

The nitrogen content of pine bark is generally very low. The nitrogen content of the pine bark after treatment with water instead of whey was only 0.18%. However, the nitrogen content of whey was about 10 times higher than that of pine bark. This is due to proteins containing nitrogen in the whey. If the pine bark adsorbs whey proteins, this increase or decrease in nitrogen content can be a good indicator of whey protein adsorption / desorption. As shown in Table 5, when the bark was treated with whey, the nitrogen content of the residue after filtration increased remarkably. Especially, when 1.25 g of bark was used, the nitrogen content increased by 19 times compared to the nitrogen content in the bark. However, using larger amounts of bark, the nitrogen content was rather reduced. This is because the amount of bark is relatively higher than that of adsorbed whey protein.

From the above results, it was confirmed that the bark was selectively adsorbed on the whey protein. Therefore, the optimum conditions for the whey protein adsorption on the bark were examined.

< Experimental Example 2> Pine bark Whey Optimization of protein adsorption

Through the above-mentioned experiment, it was confirmed that the bark has a characteristic of adsorbing whey protein. However, in the above experiment, the weight of the lyophilized whey powder was fixed at 3.175 g and distilled ion-exchanged water of liquid 10 was used for the weight of the pine bark. Therefore, it was difficult to dissolve the whey powder because the amount of distilled ion exchanged water used for adsorption decreased as the amount of bark used for adsorption decreased, and non - uniform reaction of bark and whey protein was expected during the adsorption experiment. In this experiment, the concentration of reduced whey in all samples was determined so that the solid content of the whey was the same (3.175 g of whey powder / 50 ml of distilled ion-exchanged water) to find optimal conditions for adsorbing whey proteins.

2.1 Adsorption experiments

The pine bark powder was tested in the same manner as in Experimental Example 1 on the basis of the above conditions. In the case of lyophilized whey powder, it was used in the experiment after being reduced to the same concentration as crude oil with distilled ion exchange water as described above. 53.175 g of the reduced whey solution was added to a 100 ml Erlenmeyer flask, and the mixture was shaken (reciprocating 132 times) for 5 hours in a water bath adjusted to 30 ° C. After shaking, the mixture was filtered under reduced pressure with a 1G3 glass filter, and the residue was washed with distilled ion-exchange water. The filtrate and the washing liquid were combined and lyophilized. The residue was dried overnight at 105 ° C and the residue yield was determined.

2.2 Adsorption of Whey Protein on Bark by Adsorption Time

The bark powder was fixed at 1.25 g (o d.), Which represents the highest weight gain rate in the experiment. 5 min, 15 min, 30 min, 1 h, 3 h, and 12 h of adsorption. After absorbing 53.175 g of reduced whey into a 100 ml Erlenmeyer flask, 1.25 g (od) of pine bark powder was added and shaken at 30 ° C for 5 minutes, 15 minutes, 30 minutes, 1 hour, 3 hours and 12 hours 132 times). After shaking, the mixture was filtered under reduced pressure through a 1G3 glass filter and the residue was washed with distilled ion-exchange water. The filtrate and rinse were combined and stored in a refrigerator at 4 ° C until analysis. The residue was dried in a 105 ° C dryer for 12 hours and the weight gain was measured.

2.3 Whey Protein Adsorption of Bark by Adsorption Temperature

The bark powder was fixed at 1.25 g (OD). The adsorption time was 5 minutes, 15 minutes, 30 minutes, 1 hour, 3 hours, and 12 hours. For the adsorption, 53.175 g of reduced whey was added to a 100 ml Erlenmeyer flask, and the bark powder was added to the flask. The mixture was shaken at 30 ° C, 40 ° C and 50 ° C for 5 minutes, 15 minutes, 30 minutes, 1 hour, 3 hours and 12 hours 132 reciprocations). After shaking, the mixture was filtered under reduced pressure through a 1G3 glass filter and the residue was washed with distilled ion-exchange water. The filtrate and rinse were combined and stored in a refrigerator at 4 ° C until analysis. The residue was dried in a 105 ° C dryer for 12 hours and the weight gain was measured. The same experiment as above was carried out in a water bath at 40 占 폚.

2.4 Results and discussion

Table 6 shows changes in the weight of the husks after adsorption at 30 ° C for 5 hours with different bark weights at the same conditions as the concentration of crude whey (3.175 g / 50 g of distilled ion-exchanged water). Compared with the results of Table 4 of Experimental Example 1, the weight gain rate was decreased as a whole, but the weight of adsorbed weight increased the most when 1.25 g of bark was used as in the previous results.

Table 6 below shows the weight change of wheat bran following the whey protein adsorption at the constant whey concentration.

The weight gain rate of the whey protein was investigated under the condition that the whey protein was adsorbed the most (bark 1.25 g). As a result, the weight increased by more than 10% within 5 minutes of adsorption as shown in Fig. That is, it was confirmed that the protein in whey was rapidly adsorbed at a short adsorption time. The bark amount was fixed at 1.25 g and the adsorption tendency at 30, 40, and 50 degrees of adsorption temperature was examined under the same condition as the whey concentration (3.175 g / 50 g of distilled ion exchange water). The amount of adsorption increased as the temperature at the adsorption increased as shown in Fig. That is, it was confirmed that whey protein adsorbed at 14.2% of the bark weight and 17.4% at 50 ° C were rapidly adsorbed within 5 minutes after the adsorption at 40 ° C. In other words, the higher the adsorption temperature, the faster the adsorption of protein in a short time, and the adsorption equilibrium reached almost 1 hour at 50 ℃ adsorption. On the other hand, since protein denaturation is expected at a high temperature of 50 ° C or higher, adsorption experiments at temperatures of 50 ° C or higher were not performed.

From the above results, it can be concluded that treatment of 2 or more bark by 1.25 g of bark (whey: bark = 42.54: 1) at an adsorption temperature of 50 ° C under the same condition as the concentration of crude whey (3.175 g of whey powder / 50 g of distilled ion- And it was confirmed that it was the optimum condition to adsorb whey protein.

< Experimental Example 3> Bark PAs and Of whey protein Composite

HPLC analysis and elemental analysis of the filtrate and adsorbed residue after the adsorption experiments in Experimental Examples 1 and 2 showed that the pine bark easily adsorbed whey protein in a short period of time. However, it is not known whether this adsorption is adsorption by PAs of pine bark. Based on the optimum conditions of whey protein adsorption, we investigated whether pine bark PAs with purity of more than 90% could be complexed with whey protein.

3.1 Materials

Radiate pine bark extracts and PAs were prepared from the Radiata pine bark powder used in Experimental Examples 1 and 2 described above. The hot - water extracts were prepared from hot - water extracts by adding distilled ion - exchange water to the pine bark powder of Radiata for 10 hrs at boiling temperature. PAs were prepared by adsorbing this hot water extract on Diaion HP 20 and then desorbing the adsorbed material on the resin with ethanol. The whey was the same as that used in Experimental Examples 1 and 2 above.

3.2 Whey protein adsorption of bark hydrothermal extracts and proanthocyanidins (PAs)

1.5875 g of the whey powder was taken in a 50 ml Erlenmeyer flask and then diluted with 20 g of distilled ion-exchanged water. Two reduced whey were prepared and placed in a 50 ° C water bath (SWB-10, JEIO TECH, Korea). 0.12 g of hot-water extract was dissolved in 10 ml of conical beaker and 1 ml of 50% EtOH. The conical beaker containing the sample was wrapped and placed in a 50 ° C water bath for 1 minute. The conical beaker containing the reduced whey-containing Erlenmeyer flask and the hot-water extract was taken out from the water bath at 50 ° C, and the hot-water extract was added to the reduced whey. After shaking the Erlenmeyer flask so that the whey and hot water extract were mixed well for 10 seconds, the flask was shaken (reciprocating 132 times) in a water bath at 50 ° C. 0.105 g of PAs were also treated in the same manner as the hot water extract. 0.2 ml of the mixture was taken in each Erlenmeyer flask at 5 minutes, 15 minutes, 1 hour, and 5 hours after the start of adsorption, and 5.8 ml of ultrapure water was added thereto and diluted 30 times. The diluted solution was filtered through a 0.45 μm PTFE syringe filter to obtain a 1.5 ml vial and used for the following HPLC analysis. After completion of adsorption for 5 hours, the supernatant obtained after standing in a refrigerator at 4 DEG C for 2 days was subjected to HPLC analysis in the same manner as in the above-mentioned adsorption column.

3.3 HPLC analysis

HPLC analysis of the diluted solution after the adsorption filtration was carried out under the same conditions as in Experimental Example 1 described above.

3.4 Results and discussion

FIG. 4 is a graph showing the area of whey protein peaks observed in HPLC chromatogram of the filtrate after 5 minutes, 15 minutes, 1 hour, and 5 hours after adsorption. The whey protein α-lactalbumin adsorbed 95% within 5 min of adsorption, about 80% for β-lactoglobulin A and about 70% for β-lactoglobulin B. From these results, it is clear that whey protein forms a complex with PAs, which is the main component of pine bark, and it is clear that the whey protein removal ability of the bark mentioned above is due to this PAs.

From the above results, it was expected that wheat bran PAs and whey protein complex could be a new functional food as well as economical treatment of whey in cheese farm because they have excellent whey protein adsorption characteristics on pine bark itself.

Claims

As a method for adsorbing whey proteins using polyphenols,
The method of adsorbing the whey protein comprises the steps of: preparing a polyphenol powder having a predetermined particle size prepared from pine bark;
Treating the polyphenol powder with whey to produce a polyphenolic whey protein composition; And
Isolating said polyphenolic whey protein composition,
Wherein the polyphenols are at least one selected from the group consisting of catechins, resveratrol, quercitin, and isoflavones derived from pine bark.

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The method according to claim 1,
Wherein the whey protein adsorption method is carried out at a temperature of 30 to 50 DEG C for 5 minutes to 60 minutes

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