KR101776375B9 - 피루브산 디하이드로게나아제 변이체, 이를 포함하는 미생물 및 이를 이용한 l-아미노산 생산 방법 - Google Patents

피루브산 디하이드로게나아제 변이체, 이를 포함하는 미생물 및 이를 이용한 l-아미노산 생산 방법

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Publication number
KR101776375B9
KR101776375B9 KR1020150037654A KR20150037654A KR101776375B9 KR 101776375 B9 KR101776375 B9 KR 101776375B9 KR 1020150037654 A KR1020150037654 A KR 1020150037654A KR 20150037654 A KR20150037654 A KR 20150037654A KR 101776375 B9 KR101776375 B9 KR 101776375B9
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KR
South Korea
Prior art keywords
same
microorganism
producing
amino acid
pyruvate dehydrogenase
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KR1020150037654A
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English (en)
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KR101776375B1 (ko
KR20160113377A (ko
Inventor
허란
문준옥
배현원
김형준
송성기
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씨제이제일제당 (주)
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=56919058&utm_source=***_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=KR101776375(B9) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by 씨제이제일제당 (주) filed Critical 씨제이제일제당 (주)
Priority to KR1020150037654A priority Critical patent/KR101776375B1/ko
Priority to PL16765246.0T priority patent/PL3272860T3/pl
Priority to MYPI2017703025A priority patent/MY187636A/en
Priority to HUE16765246A priority patent/HUE064139T2/hu
Priority to JP2017548259A priority patent/JP6625133B2/ja
Priority to BR112017019057-5A priority patent/BR112017019057B1/pt
Priority to ES16765246T priority patent/ES2959213T3/es
Priority to US15/557,013 priority patent/US10526586B2/en
Priority to RU2017134331A priority patent/RU2683208C1/ru
Priority to CN201680002649.4A priority patent/CN106715687B/zh
Priority to EP16765246.0A priority patent/EP3272860B1/en
Priority to PCT/KR2016/002594 priority patent/WO2016148490A1/ko
Priority to DK16765246.0T priority patent/DK3272860T5/da
Publication of KR20160113377A publication Critical patent/KR20160113377A/ko
Publication of KR101776375B1 publication Critical patent/KR101776375B1/ko
Application granted granted Critical
Priority to US16/426,453 priority patent/US20190292526A1/en
Priority to JP2019163451A priority patent/JP2020000250A/ja
Publication of KR101776375B9 publication Critical patent/KR101776375B9/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/01051—Pyruvate dehydrogenase (NADP+) (1.2.1.51)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/04—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with a disulfide as acceptor (1.2.4)
    • C12Y102/04001—Pyruvate dehydrogenase (acetyl-transferring) (1.2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/15—Corynebacterium

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
KR1020150037654A 2015-03-18 2015-03-18 피루브산 디하이드로게나아제 변이체, 이를 포함하는 미생물 및 이를 이용한 l-아미노산 생산 방법 KR101776375B1 (ko)

Priority Applications (15)

Application Number Priority Date Filing Date Title
KR1020150037654A KR101776375B1 (ko) 2015-03-18 2015-03-18 피루브산 디하이드로게나아제 변이체, 이를 포함하는 미생물 및 이를 이용한 l-아미노산 생산 방법
RU2017134331A RU2683208C1 (ru) 2015-03-18 2016-03-15 Мутант пируватдегидрогеназы, микроорганизм, содержащий мутант, и способ получения L-аминокислоты с использованием микроорганизма
EP16765246.0A EP3272860B1 (en) 2015-03-18 2016-03-15 Pyruvate dehydrogenase mutant, microorganism comprising mutant, and method for producing l-amino acid by using microorganism
HUE16765246A HUE064139T2 (hu) 2015-03-18 2016-03-15 Piruvát-dehidrogenáz-mutáns, mutánst tartalmazó mikroorganizmus, és eljárás L-aminósav elõállítására mikroorganizmus használatával
JP2017548259A JP6625133B2 (ja) 2015-03-18 2016-03-15 ピルビン酸デヒドロゲナーゼ変異体、それを含む微生物及びそれを用いるｌ−アミノ酸生産方法
BR112017019057-5A BR112017019057B1 (pt) 2015-03-18 2016-03-15 Variantes de piruvato desidrogenase, um micro-organismo que compreende as mesmas e um método para produzir l-aminoácido com o uso das mesmas
ES16765246T ES2959213T3 (es) 2015-03-18 2016-03-15 Mutante de piruvato deshidrogenasa, microorganismo que comprende el mutante y método para producir l-aminoácido usando el microorganismo
US15/557,013 US10526586B2 (en) 2015-03-18 2016-03-15 Pyruvate dehydrogenase variants, a microorganism comprising the same and a method for producing L-amino acid using the same
PL16765246.0T PL3272860T3 (pl) 2015-03-18 2016-03-15 Mutant dehydrogenazy pirogronianowej, mikroorganizm zawierający mutanta oraz sposób wytwarzania l-aminokwasu przy zastosowaniu mikroorganizmu
CN201680002649.4A CN106715687B (zh) 2015-03-18 2016-03-15 丙酮酸脱氢酶突变体、包含突变体的微生物和通过使用微生物生产l-氨基酸的方法
MYPI2017703025A MY187636A (en) 2015-03-18 2016-03-15 Pyruvate dehydrogenase variants, a microorganism comprising the same and a method for producing l-amino acid using the same
PCT/KR2016/002594 WO2016148490A1 (ko) 2015-03-18 2016-03-15 피루브산 디하이드로게나아제 변이체, 이를 포함하는 미생물 및 이를 이용한 l-아미노산 생산 방법
DK16765246.0T DK3272860T5 (da) 2015-03-18 2016-03-15 Pyruvatdehydrogenase-mutant, mikroorganisme, der omfatter mutanten, og fremgangsmåde til fremstilling af L-aminosyre ved anvendelse af mikroorganismen
US16/426,453 US20190292526A1 (en) 2015-03-18 2019-05-30 Pyruvate dehydrogenase variants, a microorganism comprising the same and a method for producing l-amino acid using the same
JP2019163451A JP2020000250A (ja) 2015-03-18 2019-09-06 ピルビン酸デヒドロゲナーゼ変異体、それを含む微生物及びそれを用いるｌ−アミノ酸生産方法

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1020150037654A KR101776375B1 (ko) 2015-03-18 2015-03-18 피루브산 디하이드로게나아제 변이체, 이를 포함하는 미생물 및 이를 이용한 l-아미노산 생산 방법

Publications (3)

Publication Number Publication Date
KR20160113377A KR20160113377A (ko) 2016-09-29
KR101776375B1 KR101776375B1 (ko) 2017-09-08
KR101776375B9 true KR101776375B9 (ko) 2022-01-11

Family

ID=56919058

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020150037654A KR101776375B1 (ko) 2015-03-18 2015-03-18 피루브산 디하이드로게나아제 변이체, 이를 포함하는 미생물 및 이를 이용한 l-아미노산 생산 방법

Country Status (13)

Country Link
US (2) US10526586B2 (ko)
EP (1) EP3272860B1 (ko)
JP (2) JP6625133B2 (ko)
KR (1) KR101776375B1 (ko)
CN (1) CN106715687B (ko)
BR (1) BR112017019057B1 (ko)
DK (1) DK3272860T5 (ko)
ES (1) ES2959213T3 (ko)
HU (1) HUE064139T2 (ko)
MY (1) MY187636A (ko)
PL (1) PL3272860T3 (ko)
RU (1) RU2683208C1 (ko)
WO (1) WO2016148490A1 (ko)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101947945B1 (ko) * 2018-01-25 2019-02-13 씨제이제일제당 (주) L-아미노산을 생산하는 코리네박테리움 속 미생물 및 이를 이용한 l-아미노산의 생산방법
KR101947959B1 (ko) * 2018-05-28 2019-02-13 씨제이제일제당 (주) 변이형 호모세린 디하이드로게나제 및 이를 이용한 호모세린 또는 호모세린 유래 l-아미노산의 생산 방법
KR102311391B1 (ko) 2020-05-21 2021-10-12 씨제이제일제당 주식회사 L- 분지쇄 아미노산 생산능이 강화된 미생물 및 이를 이용하여 l-분지쇄 아미노산을 생산하는 방법
KR102344689B1 (ko) * 2020-09-01 2021-12-29 씨제이제일제당 주식회사 L-발린 생산 미생물 및 이를 이용한 l-발린 생산 방법
US20220372451A1 (en) * 2021-04-28 2022-11-24 University Of Georgia Research Foundation, Inc. Production of pyruvate or products derived from pyruvate using modified escherichia coli
CN113249347B (zh) * 2021-07-14 2021-11-12 中国科学院天津工业生物技术研究所 丙酮酸脱氢酶的突变体及其用于生产l-氨基酸的方法
CN113430151B (zh) * 2021-08-13 2022-06-07 江苏澳创生物科技有限公司 一株谷氨酸棒杆菌及其在发酵生产l-缬氨酸中的应用
KR20230108790A (ko) * 2022-01-11 2023-07-19 대상 주식회사 L-히스티딘 생산능이 향상된 에스케리치아 속 변이주 및 이를 이용한 l-히스티딘의 생산 방법

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KR940001307B1 (ko) 1991-12-27 1994-02-19 제일제당 주식회사 L-라이신을 생산하는 신규 미생물
PE20001375A1 (es) * 1998-12-18 2000-12-14 Ajinomoto Kk Metodo para producir acido l-glutamico por fermentacion
TW200734459A (en) * 1999-10-04 2007-09-16 Ajinomoto Kk Genes for heat resistant enzymes of amino acid biosynthetic pathway derived from thermophilic coryneform bacteria
DE102005045301A1 (de) 2005-09-22 2007-04-05 Degussa Ag Verfahren zur Herstellung von organisch-chemischen Verbindungen unter Verwendung coryneformer Bakterien
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JP5756259B2 (ja) 2006-09-15 2015-07-29 シージェイ チェイルジェダン コーポレイション Ｌ−リジン生産能の向上したコリネバクテリウム属およびそれを用いたｌ−リジン生産方法
RU2496867C2 (ru) * 2011-04-25 2013-10-27 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО "АГРИ") Способ получения l-аминокислоты семейства глутамата с использованием коринеформной бактерии
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KR101721722B1 (ko) 2013-03-11 2017-04-10 씨제이제일제당 (주) L-발린 생산능이 향상된 균주 및 이를 이용한 l-발린 생산방법
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Also Published As

Publication number Publication date
JP6625133B2 (ja) 2019-12-25
DK3272860T5 (da) 2024-06-10
JP2018512132A (ja) 2018-05-17
KR101776375B1 (ko) 2017-09-08
BR112017019057B1 (pt) 2024-03-05
US20190292526A1 (en) 2019-09-26
HUE064139T2 (hu) 2024-03-28
MY187636A (en) 2021-10-05
WO2016148490A1 (ko) 2016-09-22
KR20160113377A (ko) 2016-09-29
PL3272860T3 (pl) 2024-02-05
CN106715687B (zh) 2021-04-27
ES2959213T3 (es) 2024-02-21
BR112017019057A2 (pt) 2018-04-17
JP2020000250A (ja) 2020-01-09
EP3272860A4 (en) 2018-08-08
EP3272860B1 (en) 2023-08-09
EP3272860A1 (en) 2018-01-24
US10526586B2 (en) 2020-01-07
US20180057798A1 (en) 2018-03-01
CN106715687A (zh) 2017-05-24
RU2683208C1 (ru) 2019-03-26
DK3272860T3 (da) 2023-10-30

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