KR101758102B1 - Rhodobacter capsulatus sw003 and microbial agent comprising it - Google Patents
Rhodobacter capsulatus sw003 and microbial agent comprising it Download PDFInfo
- Publication number
- KR101758102B1 KR101758102B1 KR1020170025795A KR20170025795A KR101758102B1 KR 101758102 B1 KR101758102 B1 KR 101758102B1 KR 1020170025795 A KR1020170025795 A KR 1020170025795A KR 20170025795 A KR20170025795 A KR 20170025795A KR 101758102 B1 KR101758102 B1 KR 101758102B1
- Authority
- KR
- South Korea
- Prior art keywords
- rhodobacter
- rhodobacter capsulatus
- kccm11960p
- bacteria
- accession number
- Prior art date
Links
- 241000191023 Rhodobacter capsulatus Species 0.000 title claims abstract description 27
- 230000000813 microbial effect Effects 0.000 title description 15
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims abstract description 14
- 239000005416 organic matter Substances 0.000 claims abstract description 9
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 9
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 9
- 229920002472 Starch Polymers 0.000 claims abstract description 8
- 235000019698 starch Nutrition 0.000 claims abstract description 8
- 239000008107 starch Substances 0.000 claims abstract description 8
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims abstract description 7
- 102000009123 Fibrin Human genes 0.000 claims abstract description 5
- 108010073385 Fibrin Proteins 0.000 claims abstract description 5
- BWGVNKXGVNDBDI-UHFFFAOYSA-N Fibrin monomer Chemical compound CNC(=O)CNC(=O)CN BWGVNKXGVNDBDI-UHFFFAOYSA-N 0.000 claims abstract description 5
- 229950003499 fibrin Drugs 0.000 claims abstract description 5
- 244000005700 microbiome Species 0.000 claims description 22
- 239000000203 mixture Substances 0.000 claims description 13
- 238000000034 method Methods 0.000 claims description 8
- 239000002577 cryoprotective agent Substances 0.000 claims description 4
- 238000004108 freeze drying Methods 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 3
- 239000004480 active ingredient Substances 0.000 claims description 2
- 238000009630 liquid culture Methods 0.000 claims description 2
- 239000000835 fiber Substances 0.000 claims 1
- 241000894006 Bacteria Species 0.000 description 40
- 230000000243 photosynthetic effect Effects 0.000 description 30
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 22
- 241000191025 Rhodobacter Species 0.000 description 22
- 239000002689 soil Substances 0.000 description 20
- 230000000694 effects Effects 0.000 description 16
- 239000008393 encapsulating agent Substances 0.000 description 15
- 235000019645 odor Nutrition 0.000 description 15
- 244000144972 livestock Species 0.000 description 12
- 229910021529 ammonia Inorganic materials 0.000 description 11
- 239000002609 medium Substances 0.000 description 11
- 238000012360 testing method Methods 0.000 description 11
- 238000011282 treatment Methods 0.000 description 11
- LSDPWZHWYPCBBB-UHFFFAOYSA-N Methanethiol Chemical compound SC LSDPWZHWYPCBBB-UHFFFAOYSA-N 0.000 description 10
- 241000191043 Rhodobacter sphaeroides Species 0.000 description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 10
- 238000004519 manufacturing process Methods 0.000 description 9
- GETQZCLCWQTVFV-UHFFFAOYSA-N trimethylamine Chemical compound CN(C)C GETQZCLCWQTVFV-UHFFFAOYSA-N 0.000 description 8
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 7
- 239000000654 additive Substances 0.000 description 7
- 229910000037 hydrogen sulfide Inorganic materials 0.000 description 7
- 239000010871 livestock manure Substances 0.000 description 7
- 229910052717 sulfur Inorganic materials 0.000 description 7
- 239000011593 sulfur Substances 0.000 description 7
- 241000251468 Actinopterygii Species 0.000 description 6
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- 108090000790 Enzymes Proteins 0.000 description 6
- 102000004190 Enzymes Human genes 0.000 description 6
- 239000003795 chemical substances by application Substances 0.000 description 6
- 238000000354 decomposition reaction Methods 0.000 description 6
- 229940088598 enzyme Drugs 0.000 description 6
- 210000003608 fece Anatomy 0.000 description 6
- 101000925662 Enterobacteria phage PRD1 Endolysin Proteins 0.000 description 5
- 241001465754 Metazoa Species 0.000 description 5
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 5
- 230000000996 additive effect Effects 0.000 description 5
- 244000052616 bacterial pathogen Species 0.000 description 5
- 238000005119 centrifugation Methods 0.000 description 5
- 230000006872 improvement Effects 0.000 description 5
- 230000009467 reduction Effects 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 4
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 4
- 241000282898 Sus scrofa Species 0.000 description 4
- 229910052799 carbon Inorganic materials 0.000 description 4
- 230000003247 decreasing effect Effects 0.000 description 4
- 238000004332 deodorization Methods 0.000 description 4
- 238000000855 fermentation Methods 0.000 description 4
- 230000004151 fermentation Effects 0.000 description 4
- 239000001963 growth medium Substances 0.000 description 4
- 230000036039 immunity Effects 0.000 description 4
- 230000003641 microbiacidal effect Effects 0.000 description 4
- 239000008188 pellet Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 239000000758 substrate Substances 0.000 description 4
- 239000006228 supernatant Substances 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 235000008534 Capsicum annuum var annuum Nutrition 0.000 description 3
- 241000233866 Fungi Species 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 239000002537 cosmetic Substances 0.000 description 3
- 230000007613 environmental effect Effects 0.000 description 3
- 239000007789 gas Substances 0.000 description 3
- 229910052500 inorganic mineral Inorganic materials 0.000 description 3
- 229940124561 microbicide Drugs 0.000 description 3
- 239000011707 mineral Substances 0.000 description 3
- 235000010755 mineral Nutrition 0.000 description 3
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 235000020183 skimmed milk Nutrition 0.000 description 3
- 238000005507 spraying Methods 0.000 description 3
- 239000011573 trace mineral Substances 0.000 description 3
- 235000013619 trace mineral Nutrition 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- 240000004160 Capsicum annuum Species 0.000 description 2
- 235000007862 Capsicum baccatum Nutrition 0.000 description 2
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 2
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 2
- 229920002774 Maltodextrin Polymers 0.000 description 2
- 239000005913 Maltodextrin Substances 0.000 description 2
- 241000190946 Rhodopseudomonas sp. Species 0.000 description 2
- 241000131970 Rhodospirillaceae Species 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 2
- 241000282887 Suidae Species 0.000 description 2
- UYXTWWCETRIEDR-UHFFFAOYSA-N Tributyrin Chemical compound CCCC(=O)OCC(OC(=O)CCC)COC(=O)CCC UYXTWWCETRIEDR-UHFFFAOYSA-N 0.000 description 2
- 241000209140 Triticum Species 0.000 description 2
- 235000021307 Triticum Nutrition 0.000 description 2
- 239000013543 active substance Substances 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 2
- 235000011130 ammonium sulphate Nutrition 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 229940041514 candida albicans extract Drugs 0.000 description 2
- 239000001728 capsicum frutescens Substances 0.000 description 2
- XBDQKXXYIPTUBI-UHFFFAOYSA-N dimethylselenoniopropionate Natural products CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 description 2
- PXEDJBXQKAGXNJ-QTNFYWBSSA-L disodium L-glutamate Chemical compound [Na+].[Na+].[O-]C(=O)[C@@H](N)CCC([O-])=O PXEDJBXQKAGXNJ-QTNFYWBSSA-L 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 230000002550 fecal effect Effects 0.000 description 2
- 235000013312 flour Nutrition 0.000 description 2
- 239000013505 freshwater Substances 0.000 description 2
- 238000010413 gardening Methods 0.000 description 2
- 230000005965 immune activity Effects 0.000 description 2
- 230000000968 intestinal effect Effects 0.000 description 2
- 229910052742 iron Inorganic materials 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 239000008101 lactose Substances 0.000 description 2
- 239000011777 magnesium Substances 0.000 description 2
- 229910052749 magnesium Inorganic materials 0.000 description 2
- 229940035034 maltodextrin Drugs 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 235000013372 meat Nutrition 0.000 description 2
- 239000002855 microbicide agent Substances 0.000 description 2
- 235000013923 monosodium glutamate Nutrition 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 239000000546 pharmaceutical excipient Substances 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 230000000644 propagated effect Effects 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 230000001953 sensory effect Effects 0.000 description 2
- 239000001632 sodium acetate Substances 0.000 description 2
- 235000017281 sodium acetate Nutrition 0.000 description 2
- 229940073490 sodium glutamate Drugs 0.000 description 2
- 229940074404 sodium succinate Drugs 0.000 description 2
- ZDQYSKICYIVCPN-UHFFFAOYSA-L sodium succinate (anhydrous) Chemical compound [Na+].[Na+].[O-]C(=O)CCC([O-])=O ZDQYSKICYIVCPN-UHFFFAOYSA-L 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 2
- 239000002351 wastewater Substances 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 description 1
- WJMFXQBNYLYADA-UHFFFAOYSA-N 1-(3,4-dihydroxyphenyl)-6,7-dihydroxy-1,2-dihydronaphthalene-2,3-dicarboxylic acid Chemical compound C12=CC(O)=C(O)C=C2C=C(C(O)=O)C(C(=O)O)C1C1=CC=C(O)C(O)=C1 WJMFXQBNYLYADA-UHFFFAOYSA-N 0.000 description 1
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
- 239000004382 Amylase Substances 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 241000228212 Aspergillus Species 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 240000008384 Capsicum annuum var. annuum Species 0.000 description 1
- -1 CarboxylMethyl Chemical group 0.000 description 1
- 235000014036 Castanea Nutrition 0.000 description 1
- 241001070941 Castanea Species 0.000 description 1
- 108010059892 Cellulase Proteins 0.000 description 1
- 241000191368 Chlorobi Species 0.000 description 1
- 241001142109 Chloroflexi Species 0.000 description 1
- 241000190834 Chromatiaceae Species 0.000 description 1
- 240000008067 Cucumis sativus Species 0.000 description 1
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 101000993347 Gallus gallus Ciliary neurotrophic factor Proteins 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 102000004882 Lipase Human genes 0.000 description 1
- 108090001060 Lipase Proteins 0.000 description 1
- 239000004367 Lipase Substances 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- 239000004909 Moisturizer Substances 0.000 description 1
- WHNWPMSKXPGLAX-UHFFFAOYSA-N N-Vinyl-2-pyrrolidone Chemical compound C=CN1CCCC1=O WHNWPMSKXPGLAX-UHFFFAOYSA-N 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 241000192142 Proteobacteria Species 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 241000190932 Rhodopseudomonas Species 0.000 description 1
- 206010040880 Skin irritation Diseases 0.000 description 1
- 208000031320 Teratogenesis Diseases 0.000 description 1
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 description 1
- 229910021536 Zeolite Inorganic materials 0.000 description 1
- FENRSEGZMITUEF-ATTCVCFYSA-E [Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].OP(=O)([O-])O[C@@H]1[C@@H](OP(=O)([O-])[O-])[C@H](OP(=O)(O)[O-])[C@H](OP(=O)([O-])[O-])[C@H](OP(=O)(O)[O-])[C@H]1OP(=O)([O-])[O-] Chemical compound [Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].OP(=O)([O-])O[C@@H]1[C@@H](OP(=O)([O-])[O-])[C@H](OP(=O)(O)[O-])[C@H](OP(=O)([O-])[O-])[C@H](OP(=O)(O)[O-])[C@H]1OP(=O)([O-])[O-] FENRSEGZMITUEF-ATTCVCFYSA-E 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- 239000003674 animal food additive Substances 0.000 description 1
- 230000003042 antagnostic effect Effects 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000000845 anti-microbial effect Effects 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 230000000386 athletic effect Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 239000012075 bio-oil Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 235000021466 carotenoid Nutrition 0.000 description 1
- 150000001747 carotenoids Chemical class 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 229940106157 cellulase Drugs 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 229930002875 chlorophyll Natural products 0.000 description 1
- 235000019804 chlorophyll Nutrition 0.000 description 1
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 1
- 238000009264 composting Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000001877 deodorizing effect Effects 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 235000019621 digestibility Nutrition 0.000 description 1
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 1
- 235000019797 dipotassium phosphate Nutrition 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 238000009313 farming Methods 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 230000003480 fibrinolytic effect Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000010794 food waste Substances 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000012447 hatching Effects 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 230000003308 immunostimulating effect Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 235000019421 lipase Nutrition 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000001333 moisturizer Effects 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000003895 organic fertilizer Substances 0.000 description 1
- 239000011368 organic material Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 230000020477 pH reduction Effects 0.000 description 1
- 238000009304 pastoral farming Methods 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 1
- 229920000036 polyvinylpyrrolidone Polymers 0.000 description 1
- 229910000160 potassium phosphate Inorganic materials 0.000 description 1
- 235000011009 potassium phosphates Nutrition 0.000 description 1
- 229940069328 povidone Drugs 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 235000019260 propionic acid Nutrition 0.000 description 1
- IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 description 1
- 208000023504 respiratory system disease Diseases 0.000 description 1
- 230000021749 root development Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000013535 sea water Substances 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 238000011218 seed culture Methods 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 208000017520 skin disease Diseases 0.000 description 1
- 230000036556 skin irritation Effects 0.000 description 1
- 231100000475 skin irritation Toxicity 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 229940083982 sodium phytate Drugs 0.000 description 1
- 230000003381 solubilizing effect Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 150000004763 sulfides Chemical class 0.000 description 1
- 150000003464 sulfur compounds Chemical class 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 229940124597 therapeutic agent Drugs 0.000 description 1
- 239000010455 vermiculite Substances 0.000 description 1
- 229910052902 vermiculite Inorganic materials 0.000 description 1
- 235000019354 vermiculite Nutrition 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 235000019786 weight gain Nutrition 0.000 description 1
- 239000010457 zeolite Substances 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09B—DISPOSAL OF SOLID WASTE NOT OTHERWISE PROVIDED FOR
- B09B3/00—Destroying solid waste or transforming solid waste into something useful or harmless
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
-
- C12R1/01—
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Polymers & Plastics (AREA)
- Environmental & Geological Engineering (AREA)
- Physiology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Food Science & Technology (AREA)
- General Life Sciences & Earth Sciences (AREA)
- Soil Sciences (AREA)
- Materials Engineering (AREA)
- Animal Husbandry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Water Supply & Treatment (AREA)
- Hydrology & Water Resources (AREA)
- Biodiversity & Conservation Biology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
본 발명은 단백질, 전분, 섬유소, 불용성인산 및 지방을 포함하는 유기물을 분해하는 것을 특징으로 하는 로도박터 캡슐레이터스 SW003(Rhodobacter capsulatus SW003)을 제공한다.The present invention provides Rhodobacter capsulatus SW003 (Rhodobacter capsulatus SW003) which is characterized by decomposing organic matter including protein, starch, fibrin, insoluble phosphoric acid and fat.
Description
본 발명은 로도박터 캡슐레이터스 SW003 및 이를 포함하는 미생물제에 관한 것으로, 보다 상세하게는 유기물을 분해하는 것을 특징으로 하는 로도박터 캡슐레이터스 SW003 및 이를 포함하는 미생물제에 관한 것이다.The present invention relates to Rhodobacter encapsulants SW003 and microorganisms containing the same, and more particularly to Rhodobacter encapsulants SW003 which is characterized by decomposing organic matter and a microorganism containing the same.
광합성 세균은 하천, 토양 및 해양에서 빛, 무기물, 유기물 등을 에너지원 및 탄소원으로 이용하며 생존하는 미생물이다. 이러한 광합성 세균은 유해한 탄소, 질소, 황화합물 등을 제거하는 환경정화능력을 가지며, 균체 자체의 단백질, 미량원소, 핵산, 비타민류의 함량이 매우 높고 유해세균을 억제하는 항균물질 등을 생산함으로써 친환경 농업 및 축사, 하수처리장 등에서의 악취제거에 활용도가 높은 미생물이다.Photosynthetic bacteria are living microorganisms that use light, minerals, organic matter, etc. as sources of energy and carbon in streams, soils and oceans. These photosynthetic bacteria have an environmental purification ability to remove harmful carbon, nitrogen, and sulfur compounds, and produce microbial materials that have very high content of proteins, trace elements, nucleic acids and vitamins of the cells themselves and inhibit harmful bacteria, And microorganisms that are highly utilized for the removal of offensive odors in housing and sewage treatment plants.
광합성 세균(photosynthetic bacteria, PSB)은 생산되는 광합성 색소와 전자공여체(황화수소 이용성)에 따라 홍색세균(purple bacteria)과 녹색세균(green bacteria)으로 분류되며, 홍색세균은 홍색비유황세균(purple nonsulfur bacteria)과 홍색유황세균(purple sulfur bacteria)으로 세분되고, 녹색세균은 녹색유황세균(green sulfur bacteira)과 녹색비유황세균(green nonsulfur bacteria)으로 세분된다.Photosynthetic bacteria (PSB) are classified into purple bacteria and green bacteria according to the produced photosynthetic pigments and electron donors (hydrogen sulfide availability), and red bacterium is purple nonsulfur bacteria ) And purple sulfur bacteria. Green bacteria are subdivided into green sulfur bacteira and green nonsulfur bacteria.
홍색세균은 30여 종으로 구성된 그람 음성의 구간균으로 단세포성이며, 일반적으로 이분법으로 증식하지만 일부는 출아법에 의해 증식하기도 한다. 대부분 편모로 운동성을 지니고 있으나, 비운동성인 것도 있다. 홍색유황세균은 호기성 상태에서는 광합성을 할 수 없는 절대광합성세균인 반면, 대부분의 홍색비유황세균은 암소에서 호기적으로 생육할 수 있는 화학적 유기영양체(chemoorganotroph: 유기물을 에너지원과 탄소원으로 이용하는 미생물)이다. 홍색비유황세균은 일반적으로 유기물은 존재하지만 황화물(sulfide)이 없거나 저농도로 존재하는 담수호, 연못 및 토양 등에서 서식한다. 홍색유황세균과 녹색유황세균은 제한된 특정 서식처에만 존재하기 때문에 생태계에서 광합성에 의한 생산성으로 인한 기여도는 미미한 편이다. 따라서 산업적으로 많이 이용되고 있는 것 은 홍색비유황세균으로 Rhodospirillaceae과에 속하는 로도박터 스페로이데스(Rhodobacter sphaeroides)와 로도박터 캡슐레이터스(Rhodobacter capsulatus)이다.The red bacterium is a gram-negative bacterium composed of about 30 species. It is unicellular. It is generally propagated by dichotomous method, but some of it is propagated by the method of propagation. Most are monocotyled with mobility, but some are non-athletic. The red-sulfur bacteria are absolute photosynthetic bacteria that can not be photosynthetically produced under aerobic conditions, while most non-sulfur non-sulfur bacteria are chemoorganotrophs that can be aerobically grown in the cow. Microorganisms that use organic matter as an energy source and carbon source )to be. Red non-sulfur bacteria generally live in freshwater lakes, ponds and soils where organic matter is present but not sulfides or are present in low concentrations. Red-sulfur bacteria and green-sulfur bacteria are only present in limited habitats, so their contribution from photosynthetic productivity in the ecosystem is negligible. Therefore, Rhodobacter sphaeroides and Rhodobacter capsulatus belonging to the family Rhodospirillaceae are widely used in industry.
광합성 세균은 농업, 축산, 어류양식, 환경개선, 화장품, 치료제 등 다양한 산업분야에서 활용되고 있다. 농업분야에서는 광합성 세균에 의한 질소고정으로 인하여 토양이 비옥하게 되며, 광합성 세균이 분비하는 다량의 단백질, 미량원소, 핵산 및 생리활성물질에 의해 근권환경이 개선되고 뿌리발육 등이 향상되는 효과가 있다. 특히 연작으로 인한 토양산성화의 피해를 방지하는데에도 도움이 되고 있다. 축산분야에서는 농장에서 발생되는 아세트산, 프로피온산 등과 같은 지방산, 아민류 및 황화수소계 악취발생물질을 제거하는데 이용할 수 있으며, 풍부한 영양을 공급하고 가축의 면역활성을 증진시키며 장내 병원성 세균을 억제함으로써 질병의 발생을 억제하고 가축의 생산성을 증진시킬 수 있다. 또한 광합성 세균이 생성하는 카로티노이드계 물질로 인하여 고기 및 계란의 색이 선명해지고 육질이 개선될 수 있다. 어류양식분야에서도 광합성균 자체가 단백질원이 되고 동물성 플랑크톤을 증가시킴으로써 어류의 먹이가 증가하게 되므로 치어의 부화율 및 생존율이 향상될 수 있다. 환경개선분야에서는 고농도의 폐수처리에 적합하며 담수, 해수 및 양식장의 수질 정화에 적용되고 있다. 또한, 최근에는 광합성 세균의 균체 및 배양액을 활용하여 피부보습제 등의 화장료, 피부질환이나 호흡기질환의 치료제 등으로 사용하고자 하는 시도가 이루어지고 있다.Photosynthetic bacteria are used in various industrial fields such as agriculture, animal husbandry, fish culture, environmental improvement, cosmetics, and therapeutic agents. In the field of agriculture, the soil is fertile due to the nitrogen fixation by photosynthetic bacteria, and the effect of improving the rhizosphere environment and improvement of root development by the large amount of protein, trace element, nucleic acid and physiologically active substance secreted by photosynthetic bacteria . Especially, it is helped to prevent the damage of soil acidification caused by the series. In the livestock sector, it can be used to remove fatty acids such as acetic acid and propionic acid in farms, amines and hydrogen sulfide-producing odor-producing substances. It provides abundant nutrition, improves livestock immune activity and suppresses intestinal pathogenic bacteria. And increase the productivity of livestock. In addition, carotenoids produced by photosynthetic bacteria can improve the color of meat and eggs and improve meat quality. In the field of fish culture, the photosynthetic microorganism becomes a protein source and increases the animal plankton, so that the fish feed increases, so that the hatching rate and survival rate of the fry can be improved. In the field of environmental improvement, it is suitable for treatment of high concentration wastewater and is applied to purification of fresh water, seawater and aquaculture water. In recent years, attempts have been made to use cosmetics such as skin moisturizers and treatments for skin diseases and respiratory diseases by utilizing photosynthetic bacterial cells and culture media.
등록특허 제10-1200235호는 작물의 생장 촉진 활성을 가지는 광합성 세균인 로도박터 스페로이데스, 이의 배양물, 및 상기 균주 및 배양물을 포함하는 미생물제에 대한 것으로, 이들은 작물의 생장을 촉진하고 생산성을 증대시킬 수 있는 친환경 미생물 비료제로서의 용도로 한정되어 있다. 등록특허 제10-0913611호는 광합성 세균 중 어류양식에 적합한 로도슈도모나스 루티라를 이용하여 어류의 성장촉진, 면역증강 효과 및 수질개선 효과를 갖 는 사료 첨가제 및 사료에 대하여 개시하고 있다. 등록특허 제10-1176560호 및 제10-1220922호에서는 광합성세균인 로도박터 스페로이데스를 이용하여 바이오오일 또는 수소생산능력을 극대화할 수 있는 배양방법과 조건에 대해서 기술하고 있다. 등록특허 제10-1320045호에서는 로도박터 스페로이데스로부터 수득한 박테리오클로로필 함유 균체 추출액 또는 균체를 유효성분으로 포함하는 피부 자극 완화용 또는 피부 보습용 화장료 조성물에 대하여 기술하고 있으며, 등록특허 제10-0973176호는 광합성 세균을 식품 조성물에 첨가할 경우 발생하는 악취를 제거하는 방법에 대한 것이다. 등록특허 제10-0143954호, 제10-0261747호, 제10-0381371호, 제10-0430298호 및 제10-1029346호에서는 가축의 증체 및 사료효율 개선, 가축분뇨의 악취 감소, 및 병원성 세균 감소를 위한 목적으로 하는 광합성 세균을 함유하는 첨가물 및 그 제조방법에 대하여 기재하고 있으나, 광합성 세균 단독으로 사용하는 것이 아니라 아스퍼질러스(Aspergillus)와 같은 곰팡이, 병원성 세균을 억제할 수 있는 길항균인 유산균, 바실러스, 효모 등과 같은 다른 미생물을 혼합한 복합적 기능에 대하여만 서술하고 있다.Patent No. 10-1200235 relates to Rhodobacter sphaeroides, a photosynthetic bacterium having crop growth promoting activity, a culture thereof, and a microorganism including the strain and the culture, which promote the growth of crops and improve productivity Friendly microbial fertilizer that can increase the bioavailability of the microorganism. Japanese Patent Registration No. 10-0913611 discloses a feed additive and a feed having the effect of promoting the growth of fish, improving immunity, and improving water quality by using Rhodopseudomonas lutira suitable for fish culture among photosynthetic bacteria. Japanese Patent Application Nos. 10-1176560 and 10-1220922 disclose culture methods and conditions capable of maximizing bio-oil or hydrogen production capacity using Rhodobacter sphaeroides, a photosynthetic bacterium. Japanese Patent Registration No. 10-1320045 discloses a cosmetic composition for alleviating skin irritation or skin moisturizing comprising an extract or a bacterium of bacterial chlorophyll-containing cells obtained from Rhodobacter sphaeroides as an active ingredient, 0973176 is directed to a method for eliminating odors that occur when photosynthetic bacteria are added to a food composition. 10-0143954, 10-0261747, 10-0381371, 10-0430298, and 10-1029346 disclose improvements in the growth and feed efficiency of livestock, reduction of odor of livestock manure, and reduction of pathogenic bacteria But the present invention is not limited to the use of photosynthetic bacterium alone but may be used as an antagonistic lactic acid bacterium such as Aspergillus which can inhibit fungi and pathogenic bacteria, Bacillus, yeast, and other microorganisms.
등록특허 제10-1665334호에서는 토양에서 분리된 로도박터 스페로이데스 CB 8521의 축산악취감소, 면역활성효과, 병원성 세균 항균력이 우수한 광합성세균을 기술하고 있지만 축산에 대한 악취 및 면역, 증체량 향상에 대한 방법으로써 토양 및 작물의 성장에 대한 효과는 명시되지 않았다.Although the patent No. 10-1665334 describes a photosynthetic bacterium having excellent livestock odor reduction, immunostimulatory activity, and pathogenic bacterium antimicrobial activity of Rhodobacter sphaeroides CB 8521 isolated from soil, it has been reported that the odor, immunity, The effects of the method on soil and crop growth were not specified.
자연계에 존재하는 홍색의 광합성 세균은 30여 종 이상이며, 이들은 생식처, 생리활성물질의 생산능력, 악취제거 능력, 증식능력, 토양 및 작물에 미치는 효과 등 각각의 특성이 매우 다르기 때문에 농업용 미생물, 축산, 어류양식, 수질개선 등 각 분야에 적합한 미생물의 종류는 다를 수 밖에 없다. 따라서, 순환농법적인 측면에서 토양, 가축, 가축이 배설하는 분뇨에 대한 복합적인 효과를 갖는 미생물제 선택기준에 적합한 광합성 세균 및 이를 함유하는 미생물제가 요구되고 있다.Since more than 30 photosynthetic bacteria are present in the natural world and their characteristics such as the ability to produce germplasm, physiologically active substances, odor removal ability, growth ability, soil and crops are very different, The kinds of microorganisms suitable for each field such as animal husbandry, fish culture, water quality improvement are different. Therefore, there is a demand for photosynthetic bacteria and microorganisms containing them suitable for selection of microorganisms having a complex effect on manure excreted by soil, livestock, and livestock in terms of circulatory farming.
본 발명이 해결하고자 하는 과제들은 이상에서 언급한 과제들로 제한되지 않으며, 언급되지 않은 또 다른 과제들은 아래의 기재로부터 당업자에게 명확하게 이해될 수 있을 것이다.The problems to be solved by the present invention are not limited to the above-mentioned problems, and other matters not mentioned can be clearly understood by those skilled in the art from the following description.
상기 과제를 달성하기 위하여, 본 발명은 단백질, 전분, 섬유소, 불용성인산 및 지방을 포함하는 유기물을 분해하는 로도박터 캡슐레이터스 SW003(Rhodobacter capsulatus SW003) (수탁번호:KCCM11960P)을 제공할 수 있다.In order to achieve the above object, the present invention provides Rhodobacter capsulatus SW003 (accession number: KCCM 11960P) for decomposing organic matter including protein, starch, fibrin, insoluble phosphoric acid and fat.
또한, 본 발명은 상기 로도박터 캡슐레이터스 SW003(Rhodobacter capsulatus SW003) (수탁번호:KCCM11960P)을 포함하는 미생물제 조성물을 제공할 수 있다.In addition, the present invention can provide a microbial composition comprising the Rhodobacter capsulatus SW003 (accession number: KCCM11960P).
상기 미생물제 조성물은,The microbial composition comprises
로도박터 캡슐레이터스 SW003(Rhodobacter capsulatus SW003) (수탁번호:KCCM11960P)을 액체배양하는 단계;Liquid culture of Rhodobacter capsulatus SW003 (accession number: KCCM11960P);
상기 배양된 로도박터 캡슐레이터스 SW003(Rhodobacter capsulatus SW003) (수탁번호:KCCM11960P)을 원심분리하여 균체를 회수하는 단계; 및Centrifuging the cultured Rhodobacter capsulatus SW003 (accession number: KCCM11960P) to collect the cells; And
상기 회수된 균체와 동결보호제를 1:1의 중량비로 혼합한 후 동결건조하는 단계를 포함할 수 있다.Mixing the recovered cells with a cryoprotectant at a weight ratio of 1: 1, followed by lyophilization.
본 발명의 기타 구체적인 사항들은 상세한 설명 및 도면들에 포함되어 있다.Other specific details of the invention are included in the detailed description and drawings.
본 발명의 로도박터 캡슐레이터스 SW003 (Rhodobacter capsulatus SW003) (수탁번호 : KCCM11960P) 및 이를 포함하는 미생물제는 유기물 분해력이 뛰어나 배합사료 및 축산분뇨, 식품폐수, 유기질 비료 등 고농도 유기성 폐수를 발효, 분해시켜 발효시간을 단축시킬 수 있다. 또한, 축산 현장에서 흔히 발생되는 암모니아, 황화수소, 메틸머캅탄 및 트리메틸아민과 같은 악취물질을 감소시키고, 가축에 급여시 면역활성이 증진되고 소화율 및 증체량이 증가되어 가축의 생산성을 향상시킬 수 있다. 나아가, 토양에 살포함으로써 토양을 비옥하게하고 작물의 성장 및 수확량, 유익균 증식, 면역력을 증진시켜주는 효과가 있다.The Rhodobacter capsulatus SW003 (accession number: KCCM11960P) of the present invention and the microorganism containing it are excellent in decomposition of organic matter, and fermentation and decomposition of high concentration organic wastewater such as compound feed, animal manure, food waste water and organic fertilizer The fermentation time can be shortened. In addition, it is possible to reduce odor substances such as ammonia, hydrogen sulfide, methyl mercaptan and trimethylamine, which are frequently generated in the livestock field, to improve the immune activity upon feeding the livestock, and to increase the digestibility and the amount of the livestock. Furthermore, it is effective to fertilize the soil by spraying on the soil, and to increase the growth and yield of crops, proliferation of beneficial bacteria, and immunity.
도 1은 로도박터 캡슐레이터스 SW003 (Rhodobacter capsulatus SW003) (수탁번호 : KCCM11960P) 균주를 위상차현미경으로 관찰한 사진이다.
도 2는 로도박터 캡슐레이터스 SW003 (Rhodobacter capsulatus SW003) (수탁번호 : KCCM11960P) 균주를 한천배지에서 관찰한 사진이다.1 is a photograph of a Rhodobacter capsulatus SW003 (accession number: KCCM11960P) strain observed with a phase contrast microscope.
FIG. 2 is a photograph of a strain of Rhodobacter capsulatus SW003 (accession number: KCCM11960P) observed on an agar medium.
이하, 본 발명의 실시예를 상세히 설명한다. 본 발명의 이점 및 특징, 그리고 그것들을 달성하는 방법은 상세하게 후술되어 있는 실시예들을 참조하면 명확해질 것이다. 그러나 본 발명은 이하에서 개시되는 실시예들에 한정되는 것이 아니라 서로 다른 다양한 형태로 구현될 것이며, 단지 본 실시예들은 본 발명의 개시가 완전하도록 하며, 본 발명이 속하는 기술분야에서 통상의 지식을 가진 자에게 발명의 범주를 완전하게 알려주기 위해 제공되는 것이며, 본 발명은 청구항의 범주에 의해 정의될 뿐이다. 명세서 전체에 걸쳐 동일 참조 부호는 동일 구성 요소를 지칭한다.Hereinafter, embodiments of the present invention will be described in detail. Advantages and features of the present invention and methods of achieving them will become apparent with reference to the embodiments described in detail below. The present invention may, however, be embodied in many different forms and should not be construed as being limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the invention to those skilled in the art. Is provided to fully convey the scope of the invention to those skilled in the art, and the invention is only defined by the scope of the claims. Like reference numerals refer to like elements throughout the specification.
본 명세서에서 사용된 용어는 실시예들을 설명하기 위한 것이며 본 발명을 제한하고자 하는 것은 아니다. 본 명세서에서, 단수형은 문구에서 특별히 언급하지 않는 한 복수형도 포함한다. 명세서에서 사용되는 "포함한다(comprises)" 및/또는 "이루어지다(made of)"는 언급된 구성요소, 단계, 동작 및/또는 소자는 하나 이상의 다른 구성요소, 단계, 동작 및/또는 소자의 존재 또는 추가를 배제하지 않는다. The terminology used herein is for the purpose of illustrating embodiments and is not intended to be limiting of the present invention. In the present specification, the singular form includes plural forms unless otherwise specified in the specification. As used herein, the terms "comprises" and / or "made of" means that a component, step, operation, and / or element may be embodied in one or more other components, steps, operations, and / And does not exclude the presence or addition thereof.
다른 정의가 없다면, 본 명세서에서 사용되는 모든 용어(기술 및 과학적 용어를 포함)는 본 발명이 속하는 기술분야에서 통상의 지식을 가진 자에게 공통적으로 이해될 수 있는 의미로 사용될 수 있을 것이다. 또 일반적으로 사용되는 사전에 정의되어 있는 용어들은 명백하게 특별히 정의되어 있지 않는 한 이상적으로 또는 과도하게 해석되지 않는다.Unless defined otherwise, all terms (including technical and scientific terms) used herein may be used in a sense commonly understood by one of ordinary skill in the art to which this invention belongs. Also, commonly used predefined terms are not ideally or excessively interpreted unless explicitly defined otherwise.
1. One. 로도박터Rhodobacter 캡슐레이터스Capsuleators SW003 ( SW003 ( RhodobacterRhodobacter capsulatuscapsulatus SW003) (수탁번호 : KCCM11960P) SW003) (accession number: KCCM11960P)
본 발명의 로도박터 캡슐레이터스 SW003은 홍색비유황 광합성 세균에 속하는 신규한 균주로서, 그람음성의 구간균 모양이며, 축산에 활용하기 적합한 악취발생물질 감소 능력, 장내병원성 세균에 대한 항균효능 및 면역활성능력을 가지고 있다. 본 발명의 로도박터 캡슐레이터스 SW003 균주는 한국미생물보존센터에 2017년 1월 17일자로 수탁번호 KCCM11960P 로 기탁되었다.The Rhodobacter encapsulant SW003 of the present invention is a novel strain belonging to the scarlet non-sulfur photosynthetic bacteria. It is a Gram-negative, fungus-like strain and has an ability to reduce malodor generating materials suitable for livestock production, antibacterial effect against intestinal pathogenic bacteria, It has active ability. The Rhodobacter encapsulant SW003 strain of the present invention was deposited with the Korean Microorganism Conservation Center on Jan. 17, 2017 under accession number KCCM11960P.
본 발명의 로도박터 캡슐레이터스 SW003은 축산농가 현장에서 흔히 발생되는 암모니아, 황화수소, 메틸머캅탄 및 트리메틸아민과 같은 악취물질을 90% 이상 감소시키는 효과를 나타낸다.The Rhodobacter encapsulant SW003 of the present invention has an effect of reducing the malodor substances such as ammonia, hydrogen sulfide, methyl mercaptan and trimethylamine, which are frequently generated in the livestock farming sites, by 90% or more.
또한, 고농도의 유기성폐기물인 돈분뇨에 대한 분해력이 뛰어나 발효시간을 획기적으로 단축하여 액비 부숙기간을 단축시키며 토양에 살포하여 토양을 좋게하고 작물의 성장 및 수확량 증가시키고 유익균 증식시키고 면역력을 증진시켜주는 효과가 있다.In addition, it has excellent decomposition ability to the waste manure of high concentration, which shortens the composting period by shortening the fermentation time and improves the soil by spraying on the soil, increases the growth and yield of crops, It is effective.
2. 미생물제 조성물2. Microbial composition
본 발명의 미생물제 조성물은 상기 로도박터 캡슐레이터스 SW003을 포함한다. The microbial composition of the present invention comprises the above Rhodobacter encapsulants SW003.
본 발명의 미생물제 조성물은 로도박터 캡슐레이터스 SW003을 1.0*106 ~ 1.0*1010 cfu/g 범위로 포함하는 것이 바람직하다.The microbicidal composition of the present invention preferably contains Rhodobacter encapsulant SW003 in the range of 1.0 * 10 6 to 1.0 * 10 10 cfu / g.
본 발명의 미생물제 조성물은 분뇨의 분해효능, 악취 감소, 가축의 면역력 증진으로 페사율 감소, 육성률 증가, 유기물 발효촉진 효능을 지닌 우수한 미생물제로서, 종래 사용되던 생균제를 대체하여 사용될 수 있으며, 특히 축산용, 원예용 미생물제로서 탁월하다.The microbicide composition of the present invention is an excellent microbial agent having an efficacy of decomposing manure, decreasing the odor, increasing the immunity of the domestic animal, decreasing the pessary rate, increasing the growth rate and promoting the fermentation of organic material, It is excellent as microorganism for gardening and gardening.
본 발명의 미생물제 조성물은 구체적인 용도에 따라, 다른 미생물제나 효모 또는 다른 영양성분 등을 더 포함할수 있다. 또한, 본 발명의 미생물제 조성물은 곡물 부산물을 비롯한 제오라인트, 질석 등의 부형제를 더 포함할 수 있다.The microbial composition of the present invention may further contain other microorganisms, yeast or other nutrients, depending on the specific use. In addition, the microbial composition of the present invention may further include excipients such as cereal by-products, Zeolite, vermiculite and the like.
3. 미생물제의 제조방법3. Manufacturing method of microbial agent
본 발명의 로도박터 캡슐레이터스 SW003 균주를 미생물제로 제조하는 방법은 다음과 같다.A method for producing the Rhodobacter encapsulant SW003 strain of the present invention with a microbial agent is as follows.
먼저 본 발명의 로도박터 캡슐레이터스 SW003을 24~36시간 동안 액체배양하여 종균으로 사용한다. 10㎖의 Nutrient액체배지에서 하룻밤 동안 배양한 것을 대량생산을 위한 종균으로 사용하는 것이 바람직하다. 대량생산용 배지로는 즉, 1l당 효모추출물 2.0g, 초산나트륨 0.5g, 황산암모늄 0.5g, 글루탐산나트륨 0.7g, 제이인산칼륨 0.5g, 숙신산나트륨 2.0g, 미네랄(철분, 마그네슘, 망간 등) 0.05g 및 증류수 잔부를 포함하는 액체배지를 사용하는 것이 바람직하며, 배양조건으로는 30, 조도 5,000 Lux에서 200rpm, 1vvm 상태에서 24~36시간 동안 배양하는 것이 바람직하다.First, Rhodobacter encapsulant SW003 of the present invention is liquid-cultured for 24 to 36 hours to be used as a seed culture. It is preferable to use overnight cultures in 10 ml of Nutrient liquid medium as seeds for mass production. As the medium for mass production, 2.0 g of the yeast extract per liter, 0.5 g of sodium acetate, 0.5 g of ammonium sulfate, 0.7 g of sodium glutamate, 0.5 g of potassium phosphate dibasic, 2.0 g of sodium succinate, 1 g of minerals (iron, magnesium, And the rest of the distilled water is preferably used. It is preferable that the culture medium is cultured for 30 to 36 hours under the condition of 30, the illuminance of 5,000 Lux at 200 rpm and 1 vvm.
배양이 완료된 후, 신속하게 원심분리하여 균체를 회수한 다음, 동결보호제와 1:1의 중량비로 혼합한 후 동결건조를 실시한다. 상기 동결보호제는 탈지분유, 말토덱스트린, 포도당, 유당, 밀가루, 트레할로스 및 만니톨 중 선택된 하나 이상인 것이 바람직하다.After the cultivation is completed, the cells are recovered by centrifugation at a rapid rate and then mixed with the cryoprotectant at a weight ratio of 1: 1, followed by lyophilization. The cryoprotectant is preferably at least one selected from skim milk powder, maltodextrin, glucose, lactose, wheat flour, trehalose and mannitol.
동결건조하여 제조된 미생물 고체 원말은 1.0*1010 ~ 1.0*1011 cfu/g 수준이다. 이 원말을 바람직하게는 1.0*106 ~ 1.0×108 cfu/g 수준으로 포함하도록 다른 미생물제, 부형제, 영양물질 또는 통상의 첨가제 등을 첨가하여 본 발명의 미생물제 조성물을 제조한다.The microbial solid source prepared by freeze-drying is 1.0 * 10 10 to 1.0 * 10 11 cfu / g. The microbicide composition of the present invention is prepared by adding other microorganisms, excipients, nutrients, or conventional additives to such a starter so as to preferably contain 1.0 * 10 6 to 1.0 × 10 8 cfu / g.
본 발명의 미생물제는 토양로부터 분리된 균주인 로도박터 캡슐레이터스 SW003을 이용한 것으로, 가축용 미생물제로 안전하게 사용할 수 있다.The microbial agent of the present invention uses Rhodobacter encapsulants SW003, which is a strain isolated from soil, and can be used safely as a microorganism for livestock.
[실시예][Example]
이하 구체적인 실시예를 통해 본 발명을 보다 상세히 설명한다. 그러나 다음의 실시예에 의해 본 발명의 범위가 한정되는 것은 아니며 다른 다양한 형태로 구현될 수 있다.Hereinafter, the present invention will be described in more detail with reference to specific examples. However, the scope of the present invention is not limited by the following embodiments, but may be embodied in various other forms.
<실시예 1> 광합성 세균의 분리Example 1 Isolation of Photosynthetic Bacterium
광합성 세균을 분리하기 위하여 토양 및 하천수로부터 시료를 채취하고, 실험실로 운반한 후, 4에 보관하면서 각각의 시료 1g 또는 1㎖씩 정량한 다음 9㎖의 멸균생리식염수에 넣고 순차적으로 희석하였다. 적당히 희석된 시료 0.2㎖를 R2A 배지에 도말하고 5,000lux의 광조건 및 30에서 3~5일 동안 배양하여, 배지 위에 성장한 집락(colony) 중에서 육안으로 관찰시 붉은색을 띠며 현미경관찰시에는 그람음성의 구간균 형태의 광합성 균주들을 분리하였다.In order to isolate photosynthetic bacteria, samples were taken from soil and river water, transferred to the laboratory, and stored at 4 while being weighed in 1 g or 1 ml of each sample, then placed in 9 ml of sterile physiological saline and sequentially diluted. 0.2 ml of moderately diluted specimens were plated on R2A medium and cultured at 5,000 lux for 30 days for 3 to 5 days. The colony grown on the medium showed red color when observed visually. Gram-negative Photosynthetic strains were isolated.
<실시예 2> 미생물의 동정≪ Example 2 > Identification of microorganisms
실시예 1에서 선발한 미생물에 대하여, 효소분비능력, 악취물질에 대한 탈취효과 등을 종합적으로 고려하여 최종 광합성 세균을 선발하였다. 선발된 세균을 형태학적, 생화학적 및 분자유전학적 방법인 16SrRNA 염기서열에 의해 분석한 결과, 로도박터 캡슐레이터스(Rhodobacter capsulatus) 로 확인되었다. 이 균주를 로도박터 캡슐레이터스 SW003로 명명하였다. The final photosynthetic bacteria were selected for the microorganisms selected in Example 1, taking into consideration the enzymatic secretion ability and the deodorizing effect on the malodorous substances. The selected bacteria were identified by Rhodobacter capsulatus as a result of analysis by 16SrRNA base sequence which is a morphological, biochemical and molecular genetic method. This strain was named Rhodobacter encapsulant SW003.
<실험예 1> 효소생성능 측정≪ Experimental Example 1 >
로도박터 캡슐레이터스 SW003 균주의 효소생성능을 확인하기 위하여 각 효소별 각각의 기질(Substrate)이 들어있는 배지를 사용하여 기질 분해 정도를 판정함으로써 효소 활성을 측정하였다. 본 발명의 로도박터 캡슐레이터스 SW003, 토양에서 분리한 Rhodobacter capsulatus, 하천에서 분리한 Rhodobacter sphaeroides 및 토양에서 분리한 Rhodopseudomonas sp.와 시중에서 판매되고 있는 A사, B사, C사, D사 및 E사 광합성세균이 함유되어 있는 제품을 각 구입하여 10배 희석하여 조효소액을 만들어 아래와 같이 각 효소별 각각의 기질이 들어있는 배지를 사용하여 기질 분해 정도를 판정함으로써 효소 활성을 측정하였다. 선발균주의 단백질분해효소(protease), 전분분해효소(amylase), 섬유소분해효소(Cellulase), 불용성인산 분해효소(ability phosphate solubilizing), 지방분해효소(Lipase)생성유무를 Soluble starch, Skim milk, CarboxylMethyl Cellulose, Sodium phytate, Tributyrin, Ca3(PO4)2를 각각 첨가한 한천배지에서 생기는 투명환(clear zone)의 형성 정도에 따라 효소의 활성능력을 측정하였다.In order to confirm the enzyme production ability of Rhodobacter encapsulatus SW003 strain, enzymatic activity was measured by determining the degree of substrate decomposition using a medium containing each substrate for each enzyme. Rhodobacter capsulatus isolated from the soil, Rhodobacter sphaeroides isolated from the soil, Rhodopseudomonas sp. Isolated from the soil, and commercially available A, B, C, D and E Products containing photosynthetic bacteria were purchased and diluted 10-fold to prepare crude enzyme solutions. Enzyme activities were determined by determining the extent of substrate degradation using the medium containing each substrate for each enzyme as follows. Soluble starch, Skim milk, and CarboxylMethyl (R) were tested for the presence of protease, amylase, cellulase, ability phosphate solubilizing and lipase in the selected strains. The enzyme activity was measured according to the degree of clear zone formation in agar medium supplemented with cellulose, sodium phytate, tributyrin, and Ca 3 (PO 4 ) 2 .
분해효소protein
Lytic enzyme
분해효소Starch
Lytic enzyme
분해효소fibrin
Lytic enzyme
분해효소Fat
Lytic enzyme
분해효소Insoluble phosphoric acid
Lytic enzyme
Rhodobacter capsulatusSoil separation
Rhodobacter capsulatus
Rhodobacter sphaeroidesRiver separation
Rhodobacter sphaeroides
capsulatus SW003Rhodobacter
capsulatus SW003
Rhodopseudomonas sp.Soil separation
Rhodopseudomonas sp.
Rhodobacter capsulatusCompany A
Rhodobacter capsulatus
Rhodobacter sphaeroidesCompany B
Rhodobacter sphaeroides
Rhodobacter sphaeroidesCompany C
Rhodobacter sphaeroides
Rhodobacter capsulatsCompany E
Rhodobacter capsulats
(-: 활성없음, +: 활성 있음, ++: 활성우수, +++: 활성 매우 우수)(-: no activity, +: active, ++: active, +++: very active)
효소의 활성능력 측정결과, 표 1과 같이 토양이나 하천에서 분리된 광합성세균과 타사 제품에 함유되어 있는 광합성세균은 단백질 및 전분에 대해 활성이 적고, 섬유소, 불용성인산 및 지방에 대해서는 활성이 적거나 없었으나, 이와 달리 로도박터 캡슐레이터스 SW003 균주는 단백질, 전분 및 섬유소에 대해서는 활성이 매우 우수하게 측정되었고, 또한 불용성인산 및 지방에 대해서도 활성이 우수하게 측정되었다.As shown in Table 1, the photosynthetic bacteria contained in the photosynthetic bacteria isolated from the soil or the river and the photosynthetic bacteria contained in the other products showed little activity against protein and starch, less activity against fibrin, insoluble phosphoric acid and fat In contrast, the Rhodobacter encapsulatus SW003 strain was found to have excellent activity for protein, starch and fibrin, and also for insoluble phosphoric acid and fat.
<실험예 2> 시험관내 탈취시험≪ Experimental Example 2 > Deodorization test in vitro
로도박터 캡슐레이터스 SW003 균주 배양액시료 20㎖를 5l의 반응기에 넣고 밀봉한 다음, 시험가스인 암모니아, 메틸머캅탄 및 황화수소, 트리메틸아민을 초기농도 50 μ mol/mol로 주입하고 시험가의 농도를 일정 시간별로 측정하여 이를 시료농도로 정하였다. 이때 시험가스의 농도는 KS I 2218의 검지관식 가스측정기 규정에 의해 측정하였으며, 시험 중 온도는 23±5, 상대습도는 50±10%로 유지하였다. 비교를 위하여, 시료가 없는 상태에서도 동일하게 실시하여 공시험(blank) 농도로 정하였다.20 ml of the Rhodobacter encapsulant SW003 culture broth was placed in a 5-liter reactor and sealed. Then, ammonia, methyl mercaptan, hydrogen sulfide and trimethylamine as test gases were injected at an initial concentration of 50 μmol / mol, And the sample concentration was measured. At this time, the concentration of the test gas was measured by the detector tube gas measuring instrument of KS I 2218, and the temperature was maintained at 23 ± 5 and the relative humidity at 50 ± 10% during the test. For the purpose of comparison, the blank concentration was determined in the same manner without any sample.
탈취율(%)은 하기의 식으로 결정하였으며, 그 결과를 하기 표 2에 나타내었다.The deodorization rate (%) was determined by the following formula, and the results are shown in Table 2 below.
(상기 식에서, A는 공시험 측정값이고, B는 시료의 측정값이다)(Wherein, A is a blank test value and B is a sample test value)
(μ mol/mol)Blank concentration
(mu mol / mol)
(μ mol/mol)Sample concentration
(mu mol / mol)
<NH3>ammonia
<NH 3 >
50±10 %R.H.23 ± 5
50 ± 10% RH
<(CH3)3N>Trimethylamine
<(CH 3) 3 N>
<H2S>Hydrogen sulfide
<H 2 S>
<CH3SH>Methyl mercaptan
<CH 3 SH>
상기 표 2에 나타낸 탈취시험의 결과에서 알 수 있듯이, 본 발명의 로도박터 캡슐레이터스 SW003 균주는 반응 120분 이후 암모니아와 트리메탈아민의 농도를 90% 이상 감소시켰고, 황화수소와 메틸머캅탄의 농도를 60% 이상 감소시켰다.As can be seen from the results of the deodorization test shown in Table 2, the Rhodobacter encapsulant SW003 of the present invention decreased the concentration of ammonia and trimetalamine by 90% or more after 120 minutes of reaction, and the concentration of hydrogen sulfide and methyl mercaptan By more than 60%.
<실시예 3> 로도박터 캡슐레이터스 SW003의 배양 및 미생물제 제조Example 3 Culture of Rhodobacter encapsulator SW003 and preparation of microbial agent
로도박터 캡슐레이터스 SW003을 하기와 같은 탄소원, 질소원 및 미량원소를 포함한 조성의 배지와 배양조건(배양시간, 온도, 진탕속도 등)을 사용하여 300l 발효기에서 대량생산하였다.Rhodobacter encapsulants SW003 were mass-produced in a 300 l fermenter using a culture medium containing a carbon source, a nitrogen source and trace elements as described below, and culture conditions (culture time, temperature, shaking speed, etc.).
배양액 1l당 효모추출물 2.0g, 초산나트륨 0.5g, 황산암모늄 0.5g, 글루탐산나트륨 0.7g, 제이인산칼륨 0.5g, 숙신산나트륨 2.0g, 미네랄(철분, 마그네슘, 망간 등) 0.05g을 넣고 121에서 20분간 멸균한 후, 상기 배지에서 배양한 종균을 중량대비 2% 접종하였다. 배양온도는 30이며, 0.1vvm 수준으로 산소를 공급하였다. 교반속도를 80rpm으로 유지하면서 40 ~ 44시간 동안 배양하였을 때, 6.0*109 cfu/g 이상의 생균수를 얻을 수 있었다. 고농도의 생균수를 얻기 위해서, 20시간과 30시간째에 상기의 배지를 농축하여 무균적으로 추가 투입하여 배양하였을 때에는 2.0*1010 cfu/g 이상으로 성장하였다.2.0 g of yeast extract, 0.5 g of sodium acetate, 0.5 g of ammonium sulfate, 0.7 g of sodium glutamate, 0.5 g of potassium phosphate, 2.0 g of sodium succinate and 0.05 g of minerals (iron, magnesium, manganese, etc.) After sterilization for a minute, the seeds cultured in the medium were inoculated at 2% by weight. The incubation temperature was 30, and oxygen was supplied at 0.1 vvm level. When the agitation speed was maintained at 80 rpm for 40 to 44 hours, viable cell counts of 6.0 * 10 9 cfu / g or more were obtained. In order to obtain a high number of viable cells, the culture medium was grown at a concentration of 2.0 * 10 10 cfu / g or more when the medium was enriched and aseptically added at 20 hours and 30 hours.
상기 배양액을 원심분리하여 균체를 회수한 다음, 동결보호제(5 중량% 탈지분유, 3 중량% 유당, 5 중량% 밀가루, 85 중량% 말토덱스트린 및 2 중량% 트레할로스)와 1:1의 중량비로 혼합한 후 동결건조시켰다. 동결건조된 로도박터 캡슐레이터스 SW003의 생균수를 측정한 결과, 2.0*1012 cfu/g 이었다.The culture broth was centrifuged to collect the cells, followed by mixing in a weight ratio of 1: 1 with a cryoprotecting agent (5% by weight of skim milk powder, 3% by weight of lactose, 5% by weight of wheat flour, 85% by weight of maltodextrin and 2% And then lyophilized. The viable cell count of the lyophilized Rhodobacter encapsulant SW003 was measured and found to be 2.0 * 10 12 cfu / g.
<실험예 3><Experimental Example 3>
로도박터 캡슐레이터스 SW003 (Rhodobacter capsulatus SW003) (수탁번호 : KCCM11960P) 의 돈분 분해효능, 악취감소효능을 검증하기 위하여 1000두 규모 비육돈사를 선정하여 각각 대조구 및 처리구로 구분하였다. 돈사의 형태는 무창돈사 및 슬러리 방식이었다. 1개월간 처리구에는 돈사 평당 10ml씩 로도박터 캡슐레이터스 SW003을 물에 희석하여 주당 2회 간격으로 축사내부에 안개분무를 실시하였고 대조구에는 물을 처리구와 같은 양으로 안개분무를 실시하였다. 1개월 후 돈분을 샘플링하여 돈분의 분해효능과 악취효능을 측정하였다.To examine the effect of the swine decomposing effect and the offensive odor of Rhodobacter capsulatus SW003 (accession number: KCCM11960P), 1,000 size finishing pigs were selected and classified into control and treatment groups, respectively. The form of the pilgrimage company was a kind of pilgrimage and slurry. In the treatment for 1 month, 10ml of povidone capsulatriers SW003 were diluted with water, and spraying was done inside the house twice at a rate of 2 times per week. In the control, water was sprayed in the same amount as the treatment. One month later, the pigs were sampled to determine the fecal and fecal odor efficacy.
1. 로도박터 캡슐레이터스 SW003 (Rhodobacter capsulatus SW003) (수탁번호 : KCCM11960P)에 의한 돈분 분해효능 확인1. Confirmation of the effect of swallow decomposition by Rhodobacter capsulatus SW003 (Accession No .: KCCM11960P)
처리구와 대조구에서 돈분뇨를 샘플링하여 원심분리용 튜브에 200g을 넣고 10,000prm에서 10분 원심분리 후 상등액 및 튜브하부에 가라앉은 펠렛(하등액)을 분리하여 각각의 중량을 측정하였다. 그 결과는 표 3과 같다.The pig manure was sampled from the treatment and control, and 200 g was put into a centrifuge tube. After centrifugation at 10,000 rpm for 10 minutes, pellets (lower solution) settled in the supernatant and the bottom of the tube were separated and their weights were measured. The results are shown in Table 3.
상기 결과에서 알 수 있는 바와 같이, 로도박터 캡슐레이터스 SW003 (Rhodobacter capsulatus SW003) (수탁번호 : KCCM11960P)을 살포하지 않은 대조구에 비해 처리구에서 2배 이상 고형분이 감소된 것을 알 수 있었으며, 이 결과를 통해 본 발명의 광합성세균이 돈분의 분해에 효과를 나타내는 것으로 확인되었다.As can be seen from the above results, it was found that the solid content was reduced more than 2 times in the treatment group compared to the control without application of Rhodobacter capsulatus SW003 (accession number: KCCM11960P) It was confirmed that the photosynthetic bacteria of the present invention had an effect on the decomposition of swine fungus.
2. 로도박터 캡슐레이터스 SW003 (Rhodobacter capsulatus SW003) (수탁번호 : KCCM11960P) 에 의한 악취감소효과 확인2. Identification of odor reduction effect by Rhodobacter capsulatus SW003 (accession number: KCCM11960P)
처리구와 대조구에서 돈분뇨를 샘플링하여 암모니아와 VOC의 농도를 측정하고, 직접관능법으로 악취 정도를 측정하였다. 그 결과는 표 4과 같다.The concentration of ammonia and VOC was measured by sampling the pig manure from the control and the control, and the odor level was measured by the direct sensory method. The results are shown in Table 4.
ppmammonia
ppm
ppmVOC
ppm
(1-5)Direct sensuality
(1-5)
상기 결과에서 알 수 있는 바와 같이, 로도박터 캡슐레이터스 SW003을 1달간 주 2회 평당 10ml씩 물에 희석하여 축사 내부에 살포할 경우, 물만 살포한 대조구에 비해 암모니아, VOC 농도가 3배 정도 감소했고, 직접관능을 통한 악취평가 또한 3배 이상 감소 효과를 나타내었다.As can be seen from the above results, when Rhodobacter encapsulatus SW003 was diluted with water to a concentration of 10 ml per two times a week for 1 month, the concentration of ammonia and VOC decreased by about 3 times , And odor evaluation through direct sensory evaluation showed a reduction of more than three times.
<실험예 4><Experimental Example 4>
로도박터 캡슐레이터스 SW003 (Rhodobacter capsulatus SW003) (수탁번호 : KCCM11960P) 배양물을 사료에 0.1wt% 정도 섞어 투어한 첨가구와 첨가하지 않은 무첨가구로 구분한 육계 농장에서, 사육후 마리수, 체중 및 사료섭취량을 측정하여 육성율, 증체량, 사료요구율, 생산지수를 확인하였고, 첨가구와 무첨가구의 암모니아 농도를 측정하였다. 그 결과는 표 5과 같다.Rhodobacter capsulatus SW003 (accession number: KCCM11960P) In a broiler farm divided into an additive group in which the culture was mixed with about 0.1 wt% of the feed and a non-additive group in which the additive was not added, Growth rate, body weight gain, feed conversion rate and production index were measured by measuring the intake. Ammonia concentration in the additive and non - additive samples was measured. The results are shown in Table 5.
상기 표 5의 사양시험 결과에서 알 수 있듯이, 본 발명의 미생물제를 처리한 처리구는 대조구에 비해 육성율, 증체량과 사료요구율이 개선되는 경향을 보였고 악취의 원인이 되는 암모니아 농도 또한 개선됨을 확인하였다.As can be seen from the results of the specification test shown in Table 5, the treatment groups treated with the microbicides of the present invention showed a tendency to improve the growth rate, the amount of growth and the feed conversion ratio as compared with the control group, and the ammonia concentration as a cause of the odor was also improved.
<실험예 5><Experimental Example 5>
피망, 청양고추, 오이고추에 로도박터 캡슐레이터스 SW003 배양액을 물로 300배 희석하여 10일 간격으로 3개월 엽면시비하여 첨가군과 무첨가군으로 나누어 비교한 결과 첨가군에서 과실이 커져 생산량이 증대되었고 기형발생이 현저히 감소하였다. 흰가루 등의 진행과 순 ?昰? 현상이 거이 없고 내병성이 강함이 확연이 나타났다. 과피가 두꺼워지고 과일과 잎에 윤기가 나타났다. 이 결과를 통하여 과실의 생산성이 향상되었고 유해균이 곰팡이가 억제되어 내병성이 향상되는 것을 확인하였다.In the case of Rhodobacter encapsulator SW003 medium diluted 300 times with water, green pepper, Cheongyang red pepper, and cucumber red pepper were divided into three groups of 10 days and 3 months, respectively. Teratogenesis was significantly reduced. Progression of the white powder etc. It was obvious that the phenomenon was not severe and the disease was strong. The skin thickened and the fruits and leaves appeared shiny. The results showed that the productivity of fruit was improved and that the harmful bacteria were inhibited by the fungus to improve the disease resistance.
한편, 상술한 여러 실시예에서 기재하고 있는 각종 구성량, 비율 등에 대해서는 필요에 따라 다양하게 각 구성량, 비율 등을 변경할 수 있음은 물론이다.It goes without saying that various constituent amounts, ratios, and the like described in the above-mentioned various embodiments can be variously changed as needed.
이상 본 발명의 실시예를 설명하였지만, 본 발명이 속하는 기술분야에서 통상의 지식을 가진 자는 본 발명이 그 기술적 사상이나 필수적인 특징을 변경하지 않고서 다른 구체적인 형태로 실시될 수 있다는 것을 이해할 수 있을 것이다. 그러므로 이상에서 기술한 실시예들은 모든 면에서 예시적인 것이며 한정적이 아닌 것으로 이해해야만 한다.While the present invention has been described in connection with certain exemplary embodiments, it will be understood by those skilled in the art that various changes and modifications may be made without departing from the spirit and scope of the invention as defined by the appended claims. It is therefore to be understood that the above-described embodiments are illustrative in all aspects and not restrictive.
Claims (4)
상기 조성물은 로도박터 캡슐레이터스 SW003(Rhodobacter capsulatus SW003) (수탁번호:KCCM11960P)을 1.0*106 ~ 1.0*1010 cfu/g 범위로 포함하는 미생물제 조성물.3. The method of claim 2,
Wherein the composition comprises Rhodobacter capsulatus SW003 (Accession No .: KCCM11960P) in the range of 1.0 * 10 6 to 1.0 * 10 10 cfu / g.
상기 배양된 로도박터 캡슐레이터스 SW003(Rhodobacter capsulatus SW003) (수탁번호:KCCM11960P)을 원심분리하여 균체를 회수하는 단계; 및
상기 회수된 균체와 동결보호제를 1:1의 중량비로 혼합한 후 동결건조하는 단계를 포함하는 미생물제의 제조방법.Liquid culture of Rhodobacter capsulatus SW003 (accession number: KCCM11960P);
Centrifuging the cultured Rhodobacter capsulatus SW003 (accession number: KCCM11960P) to collect the cells; And
Mixing the collected cells with a cryoprotectant at a weight ratio of 1: 1, followed by lyophilization.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020170025795A KR101758102B1 (en) | 2017-02-27 | 2017-02-27 | Rhodobacter capsulatus sw003 and microbial agent comprising it |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020170025795A KR101758102B1 (en) | 2017-02-27 | 2017-02-27 | Rhodobacter capsulatus sw003 and microbial agent comprising it |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| KR101758102B1 true KR101758102B1 (en) | 2017-07-14 |
Family
ID=59358468
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020170025795A KR101758102B1 (en) | 2017-02-27 | 2017-02-27 | Rhodobacter capsulatus sw003 and microbial agent comprising it |
Country Status (1)
| Country | Link |
|---|---|
| KR (1) | KR101758102B1 (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108101228A (en) * | 2017-12-13 | 2018-06-01 | 浙江海洋大学 | A kind of wastewater treatment microbial microbial inoculum |
| WO2019172487A1 (en) * | 2018-03-07 | 2019-09-12 | 한국과학기술원 | Microcapsules for self-healing concrete using microorganisms and method for producing same, and self-healing concrete composition containing microcapsules |
| KR102059893B1 (en) * | 2018-12-28 | 2019-12-27 | 에코바이오홀딩스 주식회사 | Microbial community for preparation of biosulfur and its use |
| KR20200022748A (en) * | 2018-08-23 | 2020-03-04 | 코스맥스 주식회사 | Composition for anti-aging comprising Rhodobacter sphaeroides strain or culture solution of the strain |
| KR20210029928A (en) * | 2019-09-09 | 2021-03-17 | 박동재 | A Handling Method of Hairtail |
| KR102361968B1 (en) * | 2021-09-29 | 2022-02-14 | 주식회사 엠케이에스 | The wastewater treatment method using compound microorganisms |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100913611B1 (en) | 2007-08-28 | 2009-08-26 | 비앤이테크(주) | Additive for fishes feed using photosynthetic bacteria and feed comprising it |
| KR101176560B1 (en) | 2012-04-04 | 2012-08-23 | 한국에너지기술연구원 | Method for producing bio-oil using photosyntheic bacteria belonging to the genus rhodobacter |
| KR101200235B1 (en) | 2010-04-20 | 2012-11-09 | 강원대학교산학협력단 | New Rhodobacter sphaeroides DAA2 and uses thereof |
| KR101220922B1 (en) | 2011-01-25 | 2013-01-11 | 한국에너지기술연구원 | Method of hydrogen production using photosynthetic bacteria |
-
2017
- 2017-02-27 KR KR1020170025795A patent/KR101758102B1/en active IP Right Grant
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100913611B1 (en) | 2007-08-28 | 2009-08-26 | 비앤이테크(주) | Additive for fishes feed using photosynthetic bacteria and feed comprising it |
| KR101200235B1 (en) | 2010-04-20 | 2012-11-09 | 강원대학교산학협력단 | New Rhodobacter sphaeroides DAA2 and uses thereof |
| KR101220922B1 (en) | 2011-01-25 | 2013-01-11 | 한국에너지기술연구원 | Method of hydrogen production using photosynthetic bacteria |
| KR101176560B1 (en) | 2012-04-04 | 2012-08-23 | 한국에너지기술연구원 | Method for producing bio-oil using photosyntheic bacteria belonging to the genus rhodobacter |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108101228A (en) * | 2017-12-13 | 2018-06-01 | 浙江海洋大学 | A kind of wastewater treatment microbial microbial inoculum |
| WO2019172487A1 (en) * | 2018-03-07 | 2019-09-12 | 한국과학기술원 | Microcapsules for self-healing concrete using microorganisms and method for producing same, and self-healing concrete composition containing microcapsules |
| KR20200022748A (en) * | 2018-08-23 | 2020-03-04 | 코스맥스 주식회사 | Composition for anti-aging comprising Rhodobacter sphaeroides strain or culture solution of the strain |
| CN110894476A (en) * | 2018-08-23 | 2020-03-20 | 科丝美诗株式会社 | Composition for preventing or improving aging comprising Rhodococcus sphaeroides strain or culture solution thereof |
| KR102094667B1 (en) | 2018-08-23 | 2020-03-30 | 코스맥스 주식회사 | Composition for anti-aging comprising Rhodobacter sphaeroides strain or culture solution of the strain |
| KR102059893B1 (en) * | 2018-12-28 | 2019-12-27 | 에코바이오홀딩스 주식회사 | Microbial community for preparation of biosulfur and its use |
| WO2020138789A1 (en) * | 2018-12-28 | 2020-07-02 | 에코바이오홀딩스 주식회사 | Microbial community for producing bio-sulfur and use thereof |
| KR20210029928A (en) * | 2019-09-09 | 2021-03-17 | 박동재 | A Handling Method of Hairtail |
| KR102370673B1 (en) * | 2019-09-09 | 2022-03-04 | 박동재 | A Handling Method of Hairtail |
| KR102361968B1 (en) * | 2021-09-29 | 2022-02-14 | 주식회사 엠케이에스 | The wastewater treatment method using compound microorganisms |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR101758102B1 (en) | Rhodobacter capsulatus sw003 and microbial agent comprising it | |
| US5364788A (en) | Pure culture of Bacillus subtilis FERM BP-3418 | |
| CN105331556B (en) | Compound micro-ecological preparation and its preparation method and application | |
| KR100418016B1 (en) | Microorganism composition using as probiotics for growth promoting nutrient | |
| KR20140019992A (en) | Organic fertilizer containing antifungal valuable microbes as effective ingredient and its preparation method | |
| KR101098823B1 (en) | Manufacturing method of microbial agent for reducing offensive odor of livestock farm and feed for hog raising including microbial agent manufactured by the same method | |
| KR102222811B1 (en) | Additive composition for fertilizer and animal feed, and manufacturing mehtod thereof | |
| CN104962490A (en) | Enteromorpha microbial agent and preparation method thereof | |
| CN104611248A (en) | Composite biological living bacteria preparation and preparation technology thereof | |
| CN106754510A (en) | A kind of bacillus subtilis and its preparation and application | |
| CN110257292A (en) | A kind of microbial bacterial agent used for aquiculture and preparation method thereof | |
| JP2003219864A (en) | Thermophilic spawn pta-1773, and ecological environment- improving material, organic fertilizer, biological agrochemical, plant, feed/feed additive, animal, crude drug, water cleaning agent, soil cleaning agent, garbage treatment agent, compost fermentation-promoting agent/ deodorizer, modulating agent for phytoremediation, antimicrobial agent, fermented food, fermented drink, medicament and preparation for biodegradable plastic production | |
| KR101665334B1 (en) | Rhodobacter sphaeroides CB 8521 strain, having the effect of reducing malodor and immune activity in livestock industry, and microbial agent using it | |
| KR101450019B1 (en) | Cattle's wastes-manure | |
| KR20140007640A (en) | Process for cultivation of culture materials disintegrable organic sludge and culture materials | |
| KR102183599B1 (en) | Feed additives for the sterilization, deodorization and promotion of decomposting of decomposted organic fertilizers and method of manufacture the same | |
| KR20210010422A (en) | Feed additives for the sterilization, deodorization and promotion of decomposting of decomposted organic fertilizers and method of manufacture the same | |
| JPH05268945A (en) | New bacillus microorganism and use thereof | |
| JP2001149023A (en) | Bioactive agent and animal feed additive using the same | |
| KR102428554B1 (en) | Additive composition for fertilizer | |
| KR100306386B1 (en) | Microorganism preparation as feed additive and producing method thereof | |
| KR101548483B1 (en) | Bacillus subtilis sw002 | |
| CN112544782A (en) | Composite microecological preparation and preparation method and application thereof | |
| JP2002306158A (en) | Composite culture material, method for producing composite culture material, precultured material, method for producing precultured material and method for producing microbial preparation | |
| KR101859006B1 (en) | Lactic acid fermentation composition with lactic acid bacteria separated form the anguilla japonica excrement, and manufacturing method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| E701 | Decision to grant or registration of patent right | ||
| GRNT | Written decision to grant |