KR101739646B1 - A composition for treatment and prevention of acne - Google Patents
A composition for treatment and prevention of acne Download PDFInfo
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- KR101739646B1 KR101739646B1 KR1020150115608A KR20150115608A KR101739646B1 KR 101739646 B1 KR101739646 B1 KR 101739646B1 KR 1020150115608 A KR1020150115608 A KR 1020150115608A KR 20150115608 A KR20150115608 A KR 20150115608A KR 101739646 B1 KR101739646 B1 KR 101739646B1
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- A61K31/19—Carboxylic acids, e.g. valproic acid
- A61K31/194—Carboxylic acids, e.g. valproic acid having two or more carboxyl groups, e.g. succinic, maleic or phthalic acid
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Abstract
The present invention relates to a composition for treating or preventing acne comprising zinc oxide and citric acid, and has an effect of increasing the antimicrobial activity against acne when zinc oxide and citric acid are used together.
Description
The present invention relates to a composition for treating or preventing acne with antimicrobial activity against acne, and more particularly to a composition for treating or preventing acne comprising zinc oxide and citric acid.
The development of science and technology has improved the quality of life and the life expectancy has increased. As interest in health and beauty increases, interest in skin beauty is increasing. But modern indiscreet development has increased the harmful ultraviolet rays reaching the surface by polluting the environment and destroying the ozone layer. The harmful ultraviolet rays accelerate skin aging by damaging DNA, proteins and lipids which are bio-constituting substances by generating reactive oxygen species (ROS) in vivo.
In order to prevent skin aging caused by the harmful ultraviolet rays, sunscreen agents are a necessity for modern people. The major components of the ultraviolet screening agent are zinc oxide (ZnO) and titanium dioxide (TiO 2 ), which have the function of blocking ultraviolet rays. Recently the zinc oxide (ZnO) and titanium dioxide through a study (TiO 2) shows antibacterial activity for the like (S. aureus) Staphylococcus aureus, Escherichia coli (E. coli) and Bacillus subtilis (B. subtilis), zinc oxide (ZnO) has been reported to exhibit higher antimicrobial activity than titanium dioxide (TiO 2 ).
Particularly, zinc oxide (ZnO) is a representative food poisoning bacteria related to the food field, such as Bacillus cereus ( B. cereus ), Enterobacter cloacae ( E. cloacae ), E. coli , Pseudomonas aeruginosa ( P. aeruginosa ), Pseudomonas fluorescens (P. fluorescens), enteritidis Salmonella (S. enteritidis), exhibits antimicrobial activity in a concentration-dependent manner against Salmonella typhimurium (S. typhimurium), and S. aureus, the particle size of the zinc oxide (ZnO), porosity, and specific surface area It has been reported that the antimicrobial activity against microorganisms changes according to the same physicochemical change.
Listeria monocytogenes ( L. monocytogenes ), E. coli , and S. cerevisiae, which were used in combination with zinc oxide (ZnO), were more effective than citric acid (CA) as antimicrobial agent in mango juice . It has been reported that the antimicrobial activity against aureus and B. cereus is increased and the antimicrobial activity is enhanced as the concentration of zinc oxide (ZnO) used together increases.
However, in this study, when antimicrobial zinc oxide (ZnO) and citric acid were used together, only antimicrobial activity of zinc oxide (ZnO) was reported. Therefore, further studies on the antimicrobial activity of zinc oxide (ZnO) and citric acid mixture at various concentrations should be conducted. In addition, it is suggested that a study on the skin fungus related to the field of cosmetics should be carried out based on the result that the mixture of zinc oxide (ZnO) and citric acid together shows antimicrobial activity against food poisoning bacteria.
An object of the present invention is to provide a composition for treating or preventing acne comprising zinc oxide and citric acid.
It is another object of the present invention to provide a mixing ratio of zinc oxide and citric acid which can exhibit an optimum antibacterial activity.
In order to achieve the above object,
The present invention provides a composition for treating or preventing acne comprising zinc oxide and citric acid.
The composition for treating or preventing acne of the present invention has an excellent antimicrobial activity against acne bacteria.
In addition, the composition for treating or preventing acne of the present invention can exhibit optimal antibacterial activity by controlling the mixing ratio of zinc oxide and citric acid.
Fig. 1 shows results of disk diffusion experiments of a composition comprising 5 wt% zinc oxide (ZnO-1) and 1, 3, 5 and 10 wt% citric acid in Example 1, respectively.
2 is a disk diffusion test result of a composition obtained by mixing 5 wt% of citric acid and 1, 3, 5 and 10 wt% of zinc oxide (ZnO-1) in Example 1, respectively.
3 shows the results of disk diffusion experiments of zinc oxide (ZnO-1) 1, 3, 5 and 10% by weight in Comparative Example 1.
4 shows the disk diffusion experiment results of
FIG. 5 is a graph showing the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of zinc oxide (ZnO-1, ZnO-2 and ZnO-3) and citric acid.
6 is a graph showing the antibacterial activity measured by the checkerboard method of Experimental Example 2. FIG.
FIG. 7 is a graph showing the antimicrobial activity measured by the time kill curve method of Experimental Example 3. FIG.
8 is a graph showing the antibacterial activity measured according to the pH of Experimental Example 4. FIG.
FIG. 9 is a graph showing the antibacterial activity measured according to the pH of Experimental Example 4. FIG.
10 is a graph showing the amount of ROS generated according to citric acid concentration.
11 is a graph showing the amount of ROS generated according to zinc oxide (ZnO-1) concentration.
FIG. 12 is a graph showing the ROS generation amount of a mixture containing zinc oxide (ZnO-1) and citric acid.
13 is a graph showing the ROS generation amount of a mixture containing zinc oxide (ZnO-1) and citric acid.
Hereinafter, the present invention will be described in more detail.
The present invention relates to a composition for treating or preventing acne comprising zinc oxide and citric acid.
Zinc oxide (ZnO) is a food poisoning bacteria related to the food industry, such as Bacillus cereus ( B. cereus ), Enterobacter cloacae , E. cloacae ), E. coli , Pseudomonas aeruginosa, P. aeruginosa), Pseudomonas fluorescein sense bacteria (Pseudomonas The present invention relates to a method for producing an antimicrobial agent which is capable of inhibiting the antimicrobial activity against S. fluorescens , P. fluorescens , Salmonella enteritidis , S. enteritidis , Salmonella typhimurium , S. typhimurium and S. aureus , It has been reported that the antimicrobial activity changes depending on physical and chemical changes such as size, porosity and specific surface area.
In addition, citric acid (citric acid, CA) is a Listeria monocytogenes, sikjungdokgyun when used in combination with zinc oxide (Listeria monocytogenes , L. monocytogenes , E. coli , S. aureus , and B. cereus . As the concentration of zinc oxide increases, the antimicrobial activity Respectively.
However, in the compositions containing zinc oxide and citric acid, only antimicrobial activity according to the concentration change of zinc oxide has been reported, and further study on the antimicrobial activity according to the concentration of zinc oxide and citric acid is needed.
In addition, since the composition containing zinc oxide and citric acid exhibits antibacterial activity against food poisoning bacteria, it is also necessary to study the antibacterial activity against the skin uptake bacteria.
Examples of skin-damaging agents that cause skin irritation include B. subtilis , E. coli , S. aureus , P. aeruginosa , and Propionibacterium acnes , P. acnes ).
The acne bacteria ( Propionibacterium acnes , and P. acnes ) are the major causative agents of acne and anaerobic gram-positive bacteria. The fungus causes inflammation of the skin via proinflammatory cytokines and accelerates the symptoms of acne by secondary infections caused by other skin diseases.
Thus, in the present invention, the acne bacteria ( Propionibacterium acnes , and P. acnes ). More specifically, it is intended to provide a composition for treating or preventing acne comprising zinc oxide and citric acid.
The zinc oxide has particles of at least one selected from the group consisting of less than 50 nm, more than 50 nm and less than 100 nm, and more than 100 nm and less than 5 탆. As the particle size is smaller, the surface area increases, Size particles are preferably used.
The composition for treating or preventing acne according to the present invention exhibits a higher antimicrobial activity when zinc oxide and citric acid are mixed with each other than when zinc oxide and citric acid are used alone, , It can exhibit higher antimicrobial activity.
More specifically, the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of acne bacteria were determined by mixing zinc oxide and citric acid with zinc oxide and citric acid , Which is a lower value.
The zinc oxide and citric acid are mixed at a weight ratio of 10: 1 to 1: 2, and when mixed at a weight ratio of 1: 1, they exhibit the highest antimicrobial activity, and most preferably, they are mixed at a weight ratio of 1: 1.
Further, in the mixing of zinc oxide and citric acid, it is preferable to use particles of less than 50 nm in zinc oxide.
Since the composition containing zinc oxide and citric acid of the present invention exhibits antibacterial activity against acne bacteria, the composition of the present invention is a composition for treating or preventing acne.
The composition for treating or preventing acne of the present invention can be administered by parenteral or application.
In addition, the composition for treating or preventing acne of the present invention may be in the form of a paste ointment, a cream, a milk, a papermaking, a powder, a penetration pad, a solution, a gel, a formulation in the form of a lotion or a suspension and a flexible lotion, Lotion, make-up or color cosmetics.
The formulations may be prepared according to conventional methods in the art and may be administered in the amounts commonly used in the cosmetics or dermatology fields.
Specific examples of the solvent or suspending agent for use in the preparation of the parenteral administration preparation, for example, an injection or suppository, include water, propylene glycol, polyethylene glycol, benzyl alcohol, ethyl oleate and lecithin.
Examples of the base used in the production of suppositories include cacao butter, oil cacao butter, laurin butter, and wipesol. The preparation method of the preparation is not particularly limited, and any of the methods commonly used in the art can be used.
In the case of injections, diluents such as water, ethyl alcohol, macrogol, propylene glycol, citric acid, acetic acid, phosphoric acid, lactic acid, sodium lactate and sulfuric acid, sodium citrate, PH adjusting agents and buffers such as sodium and sodium phosphate, and stabilizers such as sodium pyrosulfite, ethylenediamine acetic acid, thioglycolic acid and thio lactic acid. In this case, a sufficient amount of salt, glucose, mannitol, or glycerin may be added to the preparation to prepare an isotonic solution, and it is also possible to use conventional dissolution aids, no-tainting agents, or local anesthetics.
In the case of ointments, for example, in the form of pastes, creams and gels, conventionally used bases, stabilizers, wetting agents, preservatives and the like can be incorporated as required, and the components can be mixed by a conventional method . As the base, for example, white petrolatum, polyethylene, paraffin, glycerin, cellulose derivatives, polyethylene glycol, silicon and bentonite can be used. As the preservative, methyl p-hydroxybenzoate, ethyl p-oxybenzoate, propyl p-hydroxybenzoate and the like can be used. In the case of the form of a sticking agent, the above ointment, cream, gel, paste or the like can be applied to a conventional support by a conventional method. As the support, a film or a foam sheet such as a woven or nonwoven fabric made of cotton, staple fiber and chemical fiber, flexible polyvinyl chloride, polyethylene and polyurethane can be suitably used.
Hereinafter, the present invention will be described in more detail based on the following examples and experimental examples. However, the following examples are intended to be illustrative of the present invention, and the scope of the present invention is not limited thereto.
Manufacturing example 1. Culture of the strain
The strain used in the present invention was P. acnes ATCC 6919, and was distributed at the Korean Center for Microbial Conservation (KCCM).
The P. acnes Reinforced clostridial (RC) broth (Merck, Darmstadt, Germany) was used for the ATCC 6919 strain.
The P. acnes ATCC 6919 strain was stored at 4 ° C and activated for 48 hours before inoculation of the medium.
The activated strain was inoculated into the medium at 1 × 10 7 CFU / mL, sealed in an anaerobic jar using a gas pack system (Merck Anaerocult Gaspack system, Darmstadt, Germany) and incubated in a 37 ° C. incubator for 48 hours Anaerobic culture.
Thereafter, the activity of the strain was recovered and the antimicrobial activity was measured using the strain.
≪ Measurement of antimicrobial activity by disc diffusion assay >
Example 1. Composition comprising zinc oxide and citric acid
The strain cultivated in Preparation Example 1 was used after being adjusted to 1 × 10 7 colony-forming units (CFU) / mL.
The strain cultured on a plate culture medium was prepared by laminating 100 μL of each strain using a sterilizing cotton swab.
Three types of zinc oxide (ZnO) were used: less than 50 nm (ZnO-1), 50 nm or more and less than 100 nm (ZnO-2), and 100 nm or more and less than 5 μm 50, and 50 μL of a solution prepared by mixing 1, 3, 5, and 10% by weight of the solution with respect to the volume was poured into a paper disc (
In addition, citric acid (CA) was mixed at 1, 3, 5 and 10 wt% with respect to the total volume of DMSO solution, and 50 μL of the mixed solution was injected into the paper disk.
Thereafter, the zinc oxide and the citric acid solution were absorbed and then dried to volatilize the solvent.
The mixture solution was transferred onto a flat plate on which the absorbed paper disk had been coated, and then cultured. The clear zone (mm) produced around the disk was measured to observe the antibacterial activity. The results are shown in the following Table 1 Respectively.
(%, w / v)
As shown in Table 1, the smaller the particle size of zinc oxide, the higher the activity was.
In addition, the largest diameter was observed in the composition of 5 wt% of zinc oxide (ZnO-1) and 1 wt%, 3 wt%, and 5 wt% of citric acid respectively (Fig. 1) It means to depend.
A composition in which zinc oxide (ZnO-1) was mixed in an amount of 3% by weight showed the highest antimicrobial activity in a composition comprising 5% by weight of citric acid and 3, 5 and 10% by weight of zinc oxide (ZnO-1).
Comparative Example 1. Composition comprising zinc oxide
Except that zinc oxide (ZnO-1, ZnO-3 and ZnO-3) alone was used without using citric acid.
The zinc oxide solution was incubated in a state of being adhered on a flat plate plate on which the absorbed paper disk was coated, and then the clear zone (mm) produced around the disk was measured to observe the antibacterial activity. Respectively.
Comparative Example 2. Composition comprising citric acid
The same procedure as in Example 1 was carried out except that zinc oxide was not used but only citric acid was used.
The citric acid solution was incubated in a state in which the paper disk on which the absorbed solution had been absorbed was adhered on a flat plate culture plate and then the clear zone (mm) produced around the disk was measured to observe the antibacterial activity. Respectively.
(%, w / v)
As shown in Table 2, the smaller the particle size of zinc oxide was, and the higher the concentration, the higher the activity was (Fig. 3).
On the other hand, citric acid did not show a definite inhibitory effect at all concentrations. However, when the citric acid-treated portion was compared with the non-citric acid-treated portion, the growth of acne bacteria was inhibited in a concentration-dependent manner (Fig. 4).
As a result of the antimicrobial activity by the disk diffusion, it was found that when a composition containing citric acid and zinc oxide was used, citric acid and zinc oxide showed higher antimicrobial activity than when citric acid and zinc oxide alone were used.
Experimental Example 1. Minimum inhibitory concentration and minimum sterilization concentration measurement
The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of zinc oxide (ZnO-1, ZnO-2 and ZnO-3) and citric acid were measured.
The minimum inhibitory concentration and minimum bactericidal concentration were measured by Broth macrodilution assay.
The strain cultivated in Preparation Example 1 was used after being adjusted to 1.08 × 10 8 colony-forming units (CFU) / mL.
0.05, 0.1, 0.3, 0.5, 1, 2 and 3 wt% of the zinc oxide (ZnO-1, ZnO-2 and ZnO-3) and citric acid were added to the total volume of the DMSO solution .
100 [mu] L of the above mixed solution was injected into the RC broth medium, and 10 [mu] L of the above strain was injected.
The cultures were incubated at 37 ° C for 48 hours under anaerobic conditions. After incubation, the culture broth was diluted with physiological saline until visible, and then plated on RC agar and incubated at 37 ° C for 48 hours under anaerobic conditions And cultured.
The number of confirmed bacteria was calculated by the logarithmic difference in population (Log DP) formula (1).
[Equation 1]
Log DP = Log ( N / N 0 ) = (Log N ) - (Log N 0 )
N 0 is the initial number of bacteria,
N is the number of bacteria cultured for 48 hours.
In addition, MIC (Log DP ≤ 0) is the concentration at which the initial bacterium is maintained or decreased,
MIC 50 (Log DP ≤ -0.3) is the concentration at which the initial number of bacteria is reduced by 50%
MIC 90 (Log DP ≤ -1) is the concentration at which the initial number of bacteria is reduced by 90%
MBC (Log DP ≤ -4) was the concentration at which the initial number of bacteria was inhibited by 99.99%.
As a result of the measurement, the MIC of zinc oxide was 0.1 wt% for zinc oxide (ZnO-1), 0.3 wt% for zinc oxide (ZnO-2) and zinc oxide (ZnO-3) Showed high antimicrobial activity.
In addition, all the MBC of the zinc oxide was found to be 3% by weight.
The MIC of citric acid was 0.3% by weight and the MBC was 3% by weight (FIG. 5).
Experimental Example 2. Antimicrobial activity measurement by Checkerboard method
Treatment of acne including zinc oxide (ZnO-1) and citric acid by using the checkerboard method, which is a method for ascertaining whether the antimicrobial activity when two different samples are used at various concentrations at the same time, The antimicrobial activity of the composition for prevention was measured.
The strain cultivated in Preparation Example 1 was used after being adjusted to 1.08 × 10 8 colony-forming units (CFU) / mL.
The zinc oxide (ZnO-1) and citric acid were mixed at a concentration of 1 wt% based on the total volume of the DMSO solution. The mixed solution was diluted 2-fold, and finally the mixed solution was used at a concentration of 0.06 to 1 wt% Respectively.
100 [mu] L of the above mixed solution was injected into the RC broth medium, and 10 [mu] L of the above strain was injected.
The cultures were incubated at 37 ° C for 48 hours under anaerobic conditions. After incubation, the culture broth was diluted with physiological saline until visible, and then plated on RC agar and incubated at 37 ° C for 48 hours under anaerobic conditions And cultured.
The number of bacteria identified was expressed as log value.
As a result, antimicrobial activity was exhibited depending on the concentration of zinc oxide (ZnO-1), and relatively high antimicrobial activity was exhibited in a composition of zinc oxide (ZnO-1) and citric acid at a weight ratio of 1: 1 . Also, the antimicrobial activity was decreased as the mixing ratio was increased or decreased based on 1: 1 (Fig. 6).
0.5% by weight of zinc oxide (ZnO-1) inhibited 99.99% of acne bacteria when mixed with 0.25% by weight and 0.5% by weight of citric acid, and 1% by weight of zinc oxide (ZnO- 99.99% of the bacteria were inhibited.
Based on the above results, in order to confirm the potential for antimicrobial activity when the zinc oxide (ZnO-1) and citric acid of Experimental Example 1 were individually used and the potential for the changed antimicrobial activity, zinc oxide (ZnO- 1) and the concentration of zinc chloride (ZnO-1) and citric acid in Experimental Example 2 and the MBC of each concentration at which the initial bacterial count of citric acid was inhibited by 99.99% was calculated by the fractional bactericidal concentration (FBC) index formula.
&Quot; (2) "
FBC index = FBC a + FBC b
The FBC a was MBC of the sample a when used alone as the sample b, or MBC when the sample a was used alone,
The FBC b is MBC when sample b is used alone or MBC when sample b is used alone.
In addition, the FBC index value
FBC index ≤ 0.5 (synergy),
0.5 < FBC index? 4 (no interaction) and
4 < FBC index (antagonism).
The measurement results are shown in Table 3 below.
In the results of Table 3, except for the composition of zinc oxide (ZnO-1) and citric acid in an amount of 1 wt%, antimicrobial activity was synergistic.
Experimental Example 3. Measurement of Antimicrobial Activity by Time-kill Curve Method
Based on the results of Experimental Example 2, it was confirmed that when zinc oxide (ZnO-1) and citric acid were used alone, and compositions containing zinc oxide (ZnO-1) and citric acid were used, Changes were measured over time.
The zinc oxide (ZnO-1) and citric acid used alone were mixed at 0.5 wt% with respect to the total volume of the DMSO solution.
Also, in Experimental Example 2, 0.5% by weight of each of zinc oxide (ZnO-1) and citric acid, which are concentrations of inhibiting acne bacteria by 99.99%, was used.
100 [mu] L of each of the above mixed solutions was injected into RC broth medium, and 10 [mu] L of the above strain was injected.
The cultures were incubated at 37 ° C for 48 hours under anaerobic conditions. After incubation, the culture broth was diluted with physiological saline until visible, and then plated on RC agar and incubated at 37 ° C for 48 hours under anaerobic conditions And the number of cultures was checked at 0, 6, 12, 24, 36 and 48 hours after culturing.
As a result, the number of viable cells was not significantly changed when citric acid and zinc oxide (ZnO-1) were used alone and a composition containing citric acid and zinc oxide (ZnO-1) was used before 24 hours. It was confirmed that the number of viable bacteria was drastically reduced when a composition containing citric acid and zinc oxide (ZnO-1) was used as compared with that treated with citric acid and zinc oxide (ZnO-1) alone. In addition, it was confirmed that the acne bacteria were inhibited to 99.99% after 48 hours (FIG. 7).
Experimental Example 4. Measurement of Antimicrobial Activity by pH Change
Zinc oxide and citric acid at a weight ratio of 1: 1 in comparison with the case where zinc oxide and citric acid are used alone, it is desired to determine whether the composition exhibiting relatively high antimicrobial activity is a pH change depending on citric acid.
The strain cultivated in Preparation Example 1 was used after being adjusted to 1.08 × 10 8 colony-forming units (CFU) / mL.
The zinc oxide (ZnO-1) and citric acid which were used alone were mixed in an amount of 1% by weight based on the total volume of the DMSO solution. The mixed solution was diluted twice by 2 times, and finally mixed with 0.06 to 1% Solution.
In addition, the composition containing zinc oxide (ZnO-1) and citric acid was mixed in an amount of 1 wt% with respect to the total volume of the solution, and the mixed solution was diluted 2-fold to obtain a final concentration of 0.06 to 1 wt% Mixed solution was used.
100 μL of the above mixed solution was injected into the RC broth medium, and the pH of the RC broth medium was adjusted to
The cultures were incubated at 37 ° C for 48 hours under anaerobic conditions. After incubation, the culture broth was diluted with physiological saline until visible, and then plated on RC agar and incubated at 37 ° C for 48 hours under anaerobic conditions And cultured.
As a result, it was confirmed that the pH was increased as the content of zinc oxide (ZnO-1) was increased, and the pH was lowered as the content of citric acid was increased. A specific pH change could not be confirmed in a composition in which zinc oxide (ZnO-1) and citric acid were mixed at a weight ratio of 1: 1 (FIG. 8).
Further, as a result of confirming the number of bacteria cultured for 48 hours, the activity of acne bacterium was highest at
However, according to the results of Experimental Example 2, as the content of citric acid used together with zinc oxide (ZnO-1) was increased on the basis of a composition in which zinc oxide (ZnO-1) and citric acid were mixed at a weight ratio of 1: It is judged that the pH change does not directly affect the compositions containing zinc oxide (ZnO-1) and citric acid because the number of viable bacteria increases.
Experimental Example 5. Measurement of reactive oxygen species (ROS) generated by the DCFH-DA method
DCFH-DA enters the cell and is hydrolyzed with DCFH by an esterase, and is oxidized to DCF by reactive oxygen species present in the cell to exhibit fluorescence. The amount of active oxygen (ROS) formed through the difference in fluorescence intensity can be compared.
The suspension of the bacteria cultured in Preparation Example 1 was removed by a centrifuge, washed twice with PBS, and cultured for 40 minutes with 20 μM DCFH-DA.
The cultured suspension was washed twice with PBS.
Thereafter, zinc oxide (ZnO-1) 0.06, 0.13, 0.25, 0.5 and 1 wt% based on the total weight of the solution and DMSO solution containing citric acid 0.06, 0.13, 0.25, 0.5 and 1 wt% Was injected, respectively.
A solution containing 0.06, 0.13, 0.25, 0.5 and 1 wt% of citric acid in 0.25 wt% of zinc oxide (ZnO-1) and a solution containing 0.5 wt% of zinc oxide (ZnO- 1% by weight, respectively.
The ROS generated by injecting each of the above solutions and culturing for 48 hours was measured using a multimode plate reader at 490 nm excitation and 530 nm emission.
The results of using only citric acid alone showed a lower amount of ROS than the case of using no citric acid (blank) (FIG. 10).
These results indicate that citric acid is not directly related to the generation of ROS when it exhibits antibacterial activity as an antibacterial agent, and citric acid is thought to affect microorganisms by other mechanisms.
As a result of using zinc oxide (ZnO-1) alone, it was confirmed that as the concentration of zinc oxide (ZnO-1) increases, the amount of ROS generated increases.
From the above results, it was found that ROS generated by zinc oxide inhibits the activity of the acne bacterium, thereby exhibiting antibacterial activity.
As a result of using a composition containing zinc oxide (ZnO-1) and citric acid, ROS produced when zinc oxide (ZnO-1) was used alone with citric acid decreased as the concentration of citric acid increased (Figs. 12 and 13).
The above results show that the composition containing zinc oxide and citric acid does not directly affect the activity of acne bacterium due to an increase in the amount of ROS generated, but zinc ions (Zn 2 + ) decomposed by zinc oxide are increased by citric acid, And the intensity of direct stimulation of the cell membrane is more strong than that of zinc oxide alone.
Claims (5)
Characterized in that the zinc oxide is a particle of less than 50 nm,
The composition ratio of zinc oxide and citric acid may be 0.25 wt% or more and 0.25 wt% to 0.5 wt% of citric acid or 0.2 wt% to 0.5 wt% of zinc oxide, or 0.13 wt% or more of citric acid, To 1% by weight, based on the total weight of the composition, of propionibacterium acnes.
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| KR102165525B1 (en) | 2020-05-26 | 2020-10-14 | 박성은 | Composition for improving acne skin |
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| KR20170021156A (en) | 2017-02-27 |
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