KR101715764B1 - Treatment method of organic waste, and microbial agent for the same - Google Patents

Treatment method of organic waste, and microbial agent for the same Download PDF

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KR101715764B1
KR101715764B1 KR1020160057354A KR20160057354A KR101715764B1 KR 101715764 B1 KR101715764 B1 KR 101715764B1 KR 1020160057354 A KR1020160057354 A KR 1020160057354A KR 20160057354 A KR20160057354 A KR 20160057354A KR 101715764 B1 KR101715764 B1 KR 101715764B1
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organic waste
bacillus
present
food waste
treatment method
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KR1020160057354A
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Korean (ko)
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성순임
박진석
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(주)오클린테크
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
    • B09B—DISPOSAL OF SOLID WASTE
    • B09B3/00—Destroying solid waste or transforming solid waste into something useful or harmless
    • B09B3/0058—
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F9/00—Fertilisers from household or town refuse
    • C—CHEMISTRY; METALLURGY
    • C10—PETROLEUM, GAS OR COKE INDUSTRIES; TECHNICAL GASES CONTAINING CARBON MONOXIDE; FUELS; LUBRICANTS; PEAT
    • C10L—FUELS NOT OTHERWISE PROVIDED FOR; NATURAL GAS; SYNTHETIC NATURAL GAS OBTAINED BY PROCESSES NOT COVERED BY SUBCLASSES C10G, C10K; LIQUEFIED PETROLEUM GAS; ADDING MATERIALS TO FUELS OR FIRES TO REDUCE SMOKE OR UNDESIRABLE DEPOSITS OR TO FACILITATE SOOT REMOVAL; FIRELIGHTERS
    • C10L5/00—Solid fuels
    • C10L5/40—Solid fuels essentially based on materials of non-mineral origin
    • C10L5/46—Solid fuels essentially based on materials of non-mineral origin on sewage, house, or town refuse
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
    • B09B—DISPOSAL OF SOLID WASTE
    • B09B2101/00—Type of solid waste
    • B09B2101/02—Gases or liquids enclosed in discarded articles, e.g. aerosol cans or cooling systems of refrigerators
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/20—Waste processing or separation

Abstract

The present invention relates to an organic waste treatment method and a microbial material for the same, and more specifically, to an organic waste treatment method which inoculates an organic waste with Bacillus smithii, KCTC 3618 strain, and Bacillus smithii, ATCC 55404, and ferments the same at 40-80C to treat the organic waste, and to a microbial material for treating organic waste comprising the above strains. According to the present invention, organic waste such as food waste can be efficiently fermented and made into fertilizer. The organic waste treatment method of the present invention, after the initial inoculation and upon fermentation of the organic waste, simply adds new organic waste without additional inoculation, thus allowing continuous treatment of the organic waste. The method shows an excellent water reduction effect, and thus the fermented product can later be easily used as compost or post-treated.

Description

유기성 폐기물 처리방법 및 이를 위한 미생물 제제{Treatment method of organic waste, and microbial agent for the same}TECHNICAL FIELD The present invention relates to a method for treating organic wastes and a microbial agent for the same,

본 발명은 유기성 폐기물 처리방법 및 이를 위한 미생물 제제에 관한 것으로, 구체적으로 바실러스 스미시(Bacillus smithii) KCTC 3618 및 바실러스 스미시(Bacillus smithii) ATCC 55404를 유기성 폐기물에 접종하고 40 내지 80℃에서 발효시켜 유기성 폐기물을 처리하는 방법 및 이들 균주를 포함하는 유기성 폐기물 처리용 미생물 제제에 관한 것이다.The present invention relates to a method for treating organic wastes and a microorganism preparation for the same, and more particularly, to a method for treating organic wastes, which comprises inoculating Bacillus smithii KCTC 3618 and Bacillus smithii ATCC 55404 into organic wastes and fermenting them at 40 to 80 ° C A method for treating organic wastes, and a microorganism preparation for treating organic wastes including these strains.

일반적으로 가정이나 식당 등으로부터 많은 양의 음식물 쓰레기가 배출되고 있으며, 이러한 음식물 쓰레기는 재활용이 가능한 유기성 폐기물임에도 불구하고 대부분 소각 처리하거나 매립하는 방법으로 처리되어 왔다.Generally, a large amount of food waste is emitted from households and restaurants. Although such food waste is an organic waste that can be recycled, most of the food waste has been disposed of by incineration or landfill.

음식물 쓰레기는 함수율이 높아 소각하는 경우 많은 연료가 필요하게 되어 처리비용이 높아지게 되고, 이산화탄소나 다이옥신 등의 발생을 유발시켜 대기환경에 악영향을 미칠 수 있다. 매립하는 경우에도 부패되는 과정에서 나타나는 악취 등의 문제가 있으며 매립을 위한 매립용지가 필요하기 때문에 그 양이 제한적이라는 문제도 있다.Food waste has a high water content, and when it is incinerated, it requires a lot of fuel, which leads to an increase in disposal cost, and it can cause the generation of carbon dioxide, dioxin and the like, which may adversely affect the air environment. There is also a problem that the amount of landfill is limited because there is a problem of stench in the course of decay and the landfill for landfill is required.

이러한 문제로 인하여 음식물 쓰레기와 같은 유기성 폐기물을 소각하거나 매립하지 않고 효율적으로 활용하기 위한 방안들이 연구되고 있으며, 특히 미생물을 활용하여 퇴비화함으로써 농업 등의 분야에서 유용하게 활용할 수 있도록 하고 환경오염의 문제를 줄이는 일석이조의 효과를 기대하기 위한 연구가 활발히 이루어지고 있다.Due to these problems, methods for effectively utilizing organic wastes such as food wastes without incineration or landfilling have been studied. Especially, composting using microorganisms has made it possible to utilize organic wastes effectively in agricultural fields, Research is being actively carried out to expect the effect of reducing the number of snakes.

이러한 연구결과를 바탕으로 대한민국 등록특허 제10-0805036호, 제10-1207792호, 제10-0721059호 등 미생물을 이용하여 음식물 쓰레기를 처리하는 방법들이 개발된 바 있다.On the basis of these results, methods for treating food waste using microorganisms such as Korean Patent No. 10-0805036, No. 10-1207792, No. 10-0721059 have been developed.

이중 대한민국 등록특허 제10-0721059호는 바실러스 스미시(Bacillus smithii) KACC 91161P의 내염성, 내산성, 내열성 특성을 이용하여 유기성 폐기물을 고온 퇴비화하는 기술로 음식물 쓰레기와 같은 유기성 폐기물을 매우 효율적으로 퇴비화할 수 있는 장점이 있고, 감량률이 우수하다.Korean Patent No. 10-0721059 discloses a method for composting organic wastes using Bacillus smithii KACC 91161P by utilizing the salt resistance, acid resistance and heat resistance characteristics of the wastes, thereby efficiently composting organic wastes such as food wastes There is an advantage that it has, and the weight loss rate is excellent.

본 출원인은 상기와 같은 기술을 발전시켜 보다 효율적으로 유기성 폐기물을 처리할 수 있는 방법을 개발하고자 하였으며, 특히 유기성 폐기물의 처리 분야에서 가장 중요시되는 감량률을 높일 수 있는 방법을 개발하기 위하여 노력하였다.The Applicant has developed the above-described technology to develop a method for treating organic wastes more efficiently. In particular, efforts have been made to develop a method for increasing the weight loss rate, which is the most important in the field of organic waste treatment.

이의 결과 바실러스 스미시(Bacillus smithii) KCTC 3618와 바실러스 스미시(Bacillus smithii) ATCC 55404를 유기성 폐기물의 처리를 위한 미생물로 사용할 경우, 유기성 폐기물을 효율적으로 발효시켜 비료화할 수 있음은 물론이며, 특히 유기성 폐기물의 대부분을 차지하는 수분의 감량효과를 현저히 높여 결과적으로 우수한 감량률로 이어지는 효과를 기대할 수 있음을 확인하고 본 발명을 완성하게 되었다.As a result, when Bacillus smithii KCTC 3618 and Bacillus smithii ATCC 55404 are used as microorganisms for the treatment of organic wastes, organic wastes can be efficiently fermented and fertilized, It was found that the effect of reducing the water content, which occupies the majority of the waste, is remarkably increased, and as a result, an effect leading to a good weight loss rate can be expected.

대한민국 등록특허 제10-0805036호Korean Patent No. 10-0805036 대한민국 등록특허 제10-1207792호Korean Patent No. 10-1207792 대한민국 등록특허 제10-0721059호Korean Patent No. 10-0721059

따라서 본 발명의 주된 목적은 미생물을 사용하여 유기성 폐기물을 보다 효율적으로 처리하기 위한 방법을 제공하는데 있다.Accordingly, it is a main object of the present invention to provide a method for more efficiently treating organic wastes using microorganisms.

본 발명의 다른 목적은 상기 방법을 보다 용이하게 수행할 수 있도록 하는 미생물 제제 및 이의 제조방법을 제공하는데 있다.Another object of the present invention is to provide a microbial agent and a method for producing the microbial agent which can more easily perform the method.

본 발명의 한 양태에 따르면, 본 발명은 바실러스 스미시(Bacillus smithii) KCTC 3618 및 바실러스 스미시(Bacillus smithii) ATCC 55404를 유기성 폐기물에 접종하고 40 내지 80℃에서 발효시키는 것을 특징으로 하는 유기성 폐기물 처리방법을 제공한다.According to one aspect of the invention, the invention is Bacillus Sumi when (Bacillus smithii) KCTC 3618 and Bacillus Sumi when (Bacillus smithii) processing organic waste, comprising a step of inoculation the ATCC 55404 in the organic waste, and fermentation at 40 to 80 ℃ ≪ / RTI >

본 발명의 다른 양태에 따르면, 본 발명은 바실러스 스미시(Bacillus smithii) KCTC 3618 및 바실러스 스미시(Bacillus smithii) ATCC 55404를 포함하는 유기성 폐기물 처리용 미생물 제제를 제공한다.According to another aspect of the invention, the invention provides a microbial agent for treatment of organic waste containing a Bacillus Sumi when (Bacillus smithii) KCTC 3618 and Bacillus Sumi when (Bacillus smithii) ATCC 55404.

본 발명의 또 다른 양태에 따르면, 본 발명은 바실러스 스미시(Bacillus smithii) KCTC 3618 및 바실러스 스미시(Bacillus smithii) ATCC 55404를 배지에 접종하고 50 내지 80℃에서 배양하여 배양액을 수득하는 단계; 및 톱밥 또는 쌀겨에 상기 배양액을 첨가하고 50 내지 80℃에서 교반하는 단계;를 포함하는 유기성 폐기물 처리용 미생물 제제 제조방법을 제공한다.According to another aspect of the invention there is provided the method comprising the inoculation of Bacillus Sumi when (Bacillus smithii) KCTC 3618 and Bacillus Sumi when (Bacillus smithii) ATCC 55404 in a medium and cultured to give a culture medium at 50 to 80 ℃; And adding the culture medium to sawdust or rice bran and stirring the mixture at 50 to 80 DEG C. The present invention also provides a method for producing a microorganism preparation for organic waste treatment.

본 발명의 또 다른 양태에 따르면, 본 발명은 상기 미생물 제제 제조방법으로 제조된 유기성 폐기물 처리용 미생물 제제를 제공한다.According to another aspect of the present invention, there is provided a microorganism preparation for treating organic waste produced by the method for producing microorganism preparation.

본 발명에 따르면 음식물 쓰레기와 같은 유기성 폐기물을 효율적으로 발효시켜 비료화할 수 있다. 본 발명의 유기성 폐기물 처리방법은 초기에 미생물을 접종한 이후 유기성 폐기물이 발효되면 별도의 추가 미생물 접종 없이 새로운 유기성 폐기물을 추가하는 방법으로 연속적인 유기성 폐기물 처리가 가능하며, 특히 우수한 수분감량효과를 나타내어 이후 퇴비로의 이용이나 후처리를 보다 용이하게 수행할 수 있다.According to the present invention, organic wastes such as food wastes can be effectively fermented and fertilized. The method of treating organic wastes according to the present invention can treat continuous organic wastes by adding new organic wastes without addition of additional microorganisms when the organic wastes are fermented after the initial inoculation of microorganisms, It is possible to more easily perform utilization and post treatment of compost.

도 1은 본 발명의 일실시예에 따른 유기성 폐기물 처리방법을 나타낸 블록도이다.1 is a block diagram showing a method of treating organic waste according to an embodiment of the present invention.

본 발명은 바실러스 스미시(Bacillus smithii) KCTC 3618 및 바실러스 스미시(Bacillus smithii) ATCC 55404를 함께 이용함으로써 유기성 폐기물을 보다 효율적으로 처리하는 것을 특징으로 한다.The present invention is characterized in that more efficient processing of organic waste by using the Bacillus Sumi when (Bacillus smithii) KCTC 3618 and Bacillus Sumi when (Bacillus smithii) ATCC 55404 together.

본 발명에서 유기성 폐기물의 처리라 함은 유기성 폐기물의 특정 성분을 미생물이 발효하도록 함으로써 비료화 또는 연료화하는 것을 포함하며, 소각 또는 매립을 용이하게 하기 위한 전처리도 포함한다.The treatment of organic wastes in the present invention includes fertilizing or fueling microorganisms by fermenting certain components of organic wastes, and also includes pretreatment to facilitate incineration or landfilling.

본 발명에 사용되는 바실러스 스미시(Bacillus smithii) KCTC 3618는 한국생명공학연구원 생물자원센터(Korean Collection for Type Cultures, KCTC)로부터 입수할 수 있으며, 이 밖에도 일본국립기술평가원 산하 국제생물자원센터(NBRC)로부터 NBRC 15311 균주로 입수하거나 독일미생물자원센터(DSMZ)에서 DSMZ 4216 균주로도 입수할 수 있다. 균주의 배양은 입수처에서 제공하는 배양조건(배양배지 : KCTC media No. 1(nutrient agar), 배양온도 : 55℃)을 이용할 수 있으며, 또한 자당, 이스트, 카사미노 산, KH2PO4, (NH4)2SO4 가 포함되며 pH 3 ~ 7 사이로 조절되는 배지를 사용하여 50 ~ 80℃로 배양하는 방법을 이용할 수 있다. 이때 보다 바람직하게는 Sucrose 3%, yeast extract 1.5%, casamino acid 1.5%, KH2PO4 5%, (NH4)2SO4 1.5%, MgSO4·7H2O 1%, CaCl2·2H2O 0.2%(w/v)가 함유된 배지에서 배양하는 것이 좋다. Bacillus smithii KCTC 3618 used in the present invention is available from the Korean Collection for Type Cultures (KCTC) of Korea Biotechnology Research Institute, ) As strain NBRC 15311 or as strain DSMZ 4216 at the German Microorganism Resource Center (DSMZ). The culture of the strain can be carried out using the culture conditions (culture medium: KCTC media No. 1 (nutrient agar), incubation temperature: 55 ° C) provided by the receiving site and also using sucrose, yeast, casamino acid, KH 2 PO 4 , (NH 4 ) 2 SO 4 , and culturing at 50 to 80 ° C using a medium having a pH of 3 to 7 can be used. More preferred are Sucrose 3%, yeast extract 1.5%, casamino acid 1.5%, KH 2 PO 4 5%, (NH 4 ) 2 SO 4 1.5%, MgSO 4 .7H 2 O 1%, CaCl 2 .2H 2 O 0.2% (w / v) in the medium.

본 발명에 사용되는 바실러스 스미시(Bacillus smithii) ATCC 55404는 미국 균주은행(American Type Culture Collection, ATCC)으로부터 입수할 수 있으며, ATCC에서 제공하는 배양조건(배양배지 : ATCC medium 2156, 배양온도 : 30℃)을 이용하거나 상기 바실러스 스미시(Bacillus smithii) KCTC 3618의 배지 및 배양온도를 적용하여 배양할 수 있다. Bacillus smithii ATCC 55404 used in the present invention can be obtained from the American Type Culture Collection (ATCC) under the culture conditions (culture medium: ATCC medium 2156, culture temperature: 30 Lt ; 0 > C) or using the culture medium of Bacillus smithii KCTC 3618 and the culture temperature.

상기 두 균주를 유기성 폐기물에 접종하는 방법으로 균체를 접촉하거나 균주 배양물을 첨가하는 방법 등 통상의 균주 접종법을 사용할 수 있으나, 보다 효율적인 미생물의 작용을 위해서는 상기 두 균주를 미생물 제제화하여 적용하는 것이 바람직하다.Conventional strain inoculation methods such as a method of inoculating cells with the organic wastes or adding a culture of the strains may be used. However, in order to more efficiently perform the action of the microorganism, it is preferable to apply the two strains to microorganism preparation Do.

이를 위해 본 발명에서는 상기 두 균주를 배지에 접종하고 50 내지 80℃에서 배양하여 배양액을 수득하는 단계; 및 톱밥 또는 쌀겨에 상기 배양액을 첨가하고 50 내지 80℃에서 교반하는 단계;를 포함하는 유기성 폐기물 처리용 미생물 제제 제조방법을 제공한다.To this end, the method of the present invention comprises the steps of: inoculating said two strains into a culture medium and culturing at 50 to 80 ° C to obtain a culture; And adding the culture medium to sawdust or rice bran and stirring the mixture at 50 to 80 DEG C. The present invention also provides a method for producing a microorganism preparation for organic waste treatment.

이때 배지로는 각 균주의 생장이 가능한 배지라면 어떠한 것이라 사용할 수 있으나, 효율적인 배양을 위해 Sucrose 3%, yeast extract 1.5%, casamino acid 1.5%, KH2PO4 5%, (NH4)2SO4 1.5%, MgSO4·7H2O 1%, CaCl2·2H2O 0.2%(w/v)가 함유된 배지를 사용하는 것이 바람직하다. 배양기간은 12 ~ 48시간이 적당하다. 상기 두 균주는 상기와 같은 배지에 동시에 접종하여 배양할 수 있으며, 각각 별도로 배양하는 방법도 적용할 수 있다. 배양액과 톱밥 또는 쌀겨와의 혼합비율은 배양액의 중량을 기준으로 톱밥 또는 쌀겨가 10 ~ 30배 중량이 되도록 하는 것이 바람직하며, 교반은 10 ~ 30시간이 적당하다. 톱밥과 쌀겨는 수분이 거의 함유되어 있지 않기 때문에 배양액을 바로 흡수하여 배양액의 고른 혼합이 어려울 수 있으므로, 배양액을 첨가하기 전에 미리 물을 첨가하여 교반하는 것이 바람직하다. 이때 톱밥 또는 쌀겨에 대해 물은 중량기준 0.05 ~ 0.1배를 첨가하는 것이 바람직하다. 각 균주를 사용하여 최종 수득한 교반물을 혼합하면 유기성 폐기물 처리 시 상기 두 균주를 보다 효율적으로 활성화할 수 있는 형태의 미생물 제제를 제조할 수 있다.In this case, any medium that can grow each strain can be used as a medium. However, for efficient culture, Sucrose 3%, yeast extract 1.5%, casamino acid 1.5%, KH 2 PO 4 5%, (NH 4 ) 2 SO 4 1.5%, MgSO 4揃 7H 2 O 1%, CaCl 2揃 2H 2 O 0.2% (w / v) is preferably used. The incubation period is 12 to 48 hours. The two strains may be inoculated simultaneously in the same medium as described above, or separately cultured. The mixing ratio of the culture medium to the sawdust or rice bran is preferably 10 to 30 times the weight of the sawdust or the rice bran based on the weight of the culture medium, and the stirring is preferably 10 to 30 hours. Since sawdust and rice bran are almost free of moisture, it is preferable to add water and stir before adding the culture medium, since the culture medium is immediately absorbed and mixing of the culture medium may be difficult. At this time, it is preferable to add water to sawdust or rice bran by 0.05 to 0.1 times by weight. By mixing the final obtained agitated product with each strain, it is possible to prepare a microorganism preparation in which the two strains can be more efficiently activated in the treatment of organic waste.

본 발명의 미생물 제제는 상기 두 균주의 균체 또는 배양물 이외에도 균주의 보관 또는 접종을 용이하게 하기 위한 첨가물을 더 포함할 수 있다.The microorganism preparation of the present invention may further include additives for facilitating storage or inoculation of the strain in addition to the microorganism or culture of the two strains.

이하, 실시예를 통하여 본 발명을 더욱 상세히 설명하기로 한다. 이들 실시예는 단지 본 발명을 예시하기 위한 것이므로, 본 발명의 범위가 이들 실시예에 의해 제한되는 것으로 해석되지는 않는다.Hereinafter, the present invention will be described in more detail with reference to Examples. These embodiments are only for illustrating the present invention, and thus the scope of the present invention is not construed as being limited by these embodiments.

실시예 1. 바실러스 스미시 KCTC 3618 및 바실러스 스미시 ATCC 55404를 이용한 음식물 쓰레기 처리Example 1. Food waste disposal using Bacillus smith KCTC 3618 and Bacillus smithi ATCC 55404

1-1. 미생물 제제 제조1-1. Manufacture of microbial agents

증류수 15 ~ 20ℓ에 배지성분(Sucrose 3%, yeast extract 1.5%, casamino acid 1.5%, KH2PO4 5%, (NH4)2SO4 1.5%, MgSO4·7H2O 1%, CaCl2·2H2O 0.2%)(w/v)을 혼합하고 pH 조정액으로 pH를 3 ~ 7로 조정한 다음 멸균(100℃로 20분간 처리)하여 배지를 준비하였다.(NH 4 ) 2 SO 4 1.5%, MgSO 4 .7H 2 O 1%, CaCl 2 ( 2 %), and the like were added to 15 to 20 L of distilled water. The culture medium (Sucrose 3%, yeast extract 1.5%, casamino acid 1.5%, KH 2 PO 4 5% · 2H 2 O 0.2%) (w / v) were mixed, the pH was adjusted to 3 to 7 with a pH adjusting solution, and sterilized (treated at 100 ° C. for 20 minutes) to prepare a medium.

준비된 배지에 바실러스 스미시 KCTC 3618의 종균액 및 바실러스 스미스 ATCC 55404의 종균액을 각각 최종 1%(v/v)가 되도록 첨가하고 항온조에서 50 ~ 80℃로 24시간 배양하였다.Bacillus sphaericia KCTC 3618 and Bacillus Smith ATCC 55404 were added to the prepared medium to a final concentration of 1% (v / v), respectively, and cultured in a thermostatic chamber at 50 to 80 ° C for 24 hours.

분해기에서 50 ~ 80℃로 4시간 동안 물 20 ~ 30ℓ와 교반한 삼목 톱밥 300 ~ 400ℓ에 상기 배양을 통해 수득한 배양액을 골고루 붓고 50 ~ 80℃에서 20시간 동안 교반하여 유기성 폐기물을 처리하는데 사용하기 위한 미생물 제제를 제조하였다.In the digestor, the culture solution obtained through the above culturing was poured evenly into 300-400 L of cedar sawdust stirred at 50 ~ 80 ° C for 4 hours with 20 ~ 30 L of water, and stirred at 50 ~ 80 ° C for 20 hours to be used for treating organic waste . ≪ / RTI >

1-2. 음식물 쓰레기 처리1-2. Food waste disposal

발효탱크에 상기 1-1에서 제조한 미생물제제 2ℓ 및 음식물 쓰레기 2ℓ를 넣고 40 ~ 80℃에서 교반하는 것을 시작으로, 24 ~ 48시간 간격으로 음식물 쓰레기를 2ℓ 씩 추가하여 같은 조건으로 연속적으로 교반하면서 음식물 쓰레기를 발효시켰다.2 liters of the microorganism preparation prepared in the above 1-1 and 2 liters of the food garbage prepared in the above 1-1 were added to the fermentation tank and the mixture was stirred at 40 to 80 DEG C and 2 liters of food waste was added at intervals of 24 to 48 hours, Food waste was fermented.

새로운 음식물 쓰레기를 추가하기 전에 발효가 완료된 음식물 쓰레기가 발효탱크에 가득 차 있으면 부피기준 약 30% 정도를 남기고 나머지를 발효탱크에서 수거한 다음 새로운 음식물 쓰레기를 추가하는 방법으로 연속적으로 음식물 쓰레기를 처리하였다. 발효가 완료된 음식물 쓰레기는 퇴비로 사용하였다.If the fermentation tank is filled with fermented food waste before adding new food waste, the food waste is continuously treated by adding about 30% by volume of the fermentation tank, collecting the rest from the fermentation tank, and adding new food waste . The fermented food waste was used as compost.

이때 음식물 쓰레기는 24 ~ 48시간 발효된 이후 부피기준 약 90% 정도의 감량률을 보이는 것으로 나타났다.In this case, after the fermentation for 24 ~ 48 hours, the food waste showed a reduction rate of about 90% by volume.

비교예 1. 바실러스 스미시 KACC 91161P를 이용한 음식물 쓰레기 처리Comparative Example 1. Bacillus smithi Food waste disposal using KACC 91161P

비교를 위해 기존에 개발된 음식물 쓰레기 처리방법 중 감량률이 가장 우수한 것으로 알려진 대한민국 등록특허 제10-0721059호의 방법을 사용하였다. 상기 실시예 1과 동일한 방법으로 음식물 쓰레기를 처리하되, 미생물로 바실러스 스미시 KACC 91161P를 사용하였다.For comparison, the method of Korean Patent No. 10-0721059, which is known to have the highest weight loss rate among the food waste disposal methods developed previously, was used. Food waste was treated in the same manner as in Example 1, except that Bacillus smith KACC 91161P was used as the microorganism.

실시예 2. 음식물 쓰레기 처리물의 특성 분석Example 2. Characterization of Food Waste Treated Water

상기 실시예 1 및 비교예 1을 통해 발효된 음식물 쓰레기의 특성을 분석하였다. 분석을 위해 실시예 1 및 비교예 1에 적용한 음식물 쓰레기는 모두 식물성 음식물 쓰레기로 수분함량이 약 85%인 것을 사용하였으며, 각각 15일간 운영된 이후의 음식물 쓰레기 발효물을 분석대상으로 하였다.The characteristics of the food wastes fermented through Example 1 and Comparative Example 1 were analyzed. For the analysis, the food waste used in Example 1 and Comparative Example 1 was vegetable food waste having a moisture content of about 85%, and food waste collected after 15 days of operation was analyzed.

실시예 1을 통해 처리된 음식물 쓰레기의 분석은 에이티분석센타(주)(충북 괴산군 청안면 광장로 소재)에 의뢰하였으며, 농촌진흥청에서 제시하는 비료의 품질검사방법 및 시료채취기준에 준하여 각 함량을 분석하였다. 이의 결과는 표 1과 같다.The analysis of the food waste treated through Example 1 was commissioned by ETIS Analysis Center Co., Ltd. (located in Cheongan-myeon Square, Goesan-gun, Chungbuk Province). The contents of each food waste were analyzed according to the quality inspection method of the fertilizer Respectively. The results are shown in Table 1.

비교예 1을 통해 처리된 음식물 쓰레기의 분석은 제일분석센타(경기도 안산시 단원구 성곡동 소재)에 의뢰하였으며, 농촌진흥청에서 제시하는 비료의 품질검사방법 및 시료채취기준에 준하여 각 함량을 분석하였다. 이의 결과는 표 2와 같다.Analysis of the food waste treated in Comparative Example 1 was commissioned to the First Analysis Center (Sunggok-dong, Danwon-gu, Ansan, Gyeonggi-do) and the content of each food waste was analyzed in accordance with the method of quality inspection of the fertilizer and sampling standards. The results are shown in Table 2.

이들 결과를 비교해 보면, 실시예 1의 음식물 쓰레기 처리물의 수분함량(16.29%)이 비교예 1(25.27%)에 비해 현저히 낮은 것을 알 수 있다. 이는 본 발명의 방법이 특히 유기성 폐기물의 수분감량에서 우수한 효과를 나타내며 이에 따라 우수한 감량률을 달성할 수 있다는 것을 의미한다.Comparing these results, it can be seen that the water content (16.29%) of the food waste treated in Example 1 is significantly lower than that of Comparative Example 1 (25.27%). This means that the process of the present invention is particularly effective in reducing the moisture loss of organic wastes and thus achieving a good reduction rate.

실시예 1의 음식물 쓰레기 처리물의 분석결과The analysis result of the food waste treated in Example 1 분석항목(단위)Analysis Item (Unit) 공정규격Process standard 분석결과Analysis 규격standard 유기물(%)Organic matter (%) 30이상30 or more 78.3878.38


유해
성분


harmfulness
ingredient 비소(㎎/㎏)(건물중)Arsenic (㎎ / ㎏) (in buildings) 45이하45 or less 불검출Non-detection 카드뮴(㎎/㎏)(건물중)Cadmium (㎎ / ㎏) (in buildings) 5이하5 or less 1.051.05 수은(㎎/㎏)(건물중)Mercury (㎎ / ㎏) (in buildings) 2이하2 or less 불검출Non-detection 납(㎎/㎏)(건물중)Lead (㎎ / ㎏) (in building) 130이하130 or less 1.681.68 크롬(㎎/㎏)(건물중)Chromium (㎎ / ㎏) (in building) 200이하200 or less 9.319.31 구리(㎎/㎏)(건물중)Copper (㎎ / ㎏) (in building) 360이하Less than 360 9.489.48 니켈(㎎/㎏)(건물중)Nickel (㎎ / ㎏) (in buildings) 45이하45 or less 4.004.00 아연(㎎/㎏)(건물중)Zinc (㎎ / ㎏) (in building) 900이하900 or less 17.6317.63 대장균O157:H7E. coli O157: H7 불검출Non-detection 불검출Non-detection 살모넬라Salmonella 불검출Non-detection 불검출Non-detection
그밖의
규격
Other
standard 유기물대 질소의 비Ratio of organic matter to nitrogen 45이하45 or less 30.1430.14 염분(%)(건물중)Salinity (%) (in buildings) 2.0이하2.0 or less 2.662.66 수분(%)moisture(%) 55이하55 or less 16.2916.29 부숙도(콤백법)Composting (compaction method) 부숙완료 이상Abnormal completion 부숙완료Compartmentalized 염산불용해물(%)Hydrochloric acid insoluble matter (%) 25이하25 or less 0.690.69 공정규격
외Process standard
outside 질소전량(%)Total nitrogen (%) -- 2.602.60 인산전량(%)Total amount of phosphoric acid (%) -- 0.690.69 칼리전량(%)Total amount of carly (%) -- 0.820.82

비교예 1의 음식물 쓰레기 처리물의 분석결과The analysis result of the food waste treated in Comparative Example 1 항목Item 규격standard 결과result 질소(%)nitrogen(%) 2.732.73 인산(%)Phosphoric acid (%) 1.451.45 가리(%)weir(%) 1.011.01 수분(%)moisture(%) 55 이하55 or less 25.2725.27 유기물(%)Organic matter (%) 67.0267.02 유기물대질소비Organic material consumption 50 이하Less than 50 24.5424.54 유기물(%)Organic matter (%)



건물중에
대하여



In the building
about 50 이상Over 50 90.5690.56 비소(㎎/㎏)Arsenic (mg / kg) 45 이하45 or less 불검출Non-detection 카드뮴(㎎/㎏)Cadmium (mg / kg) 5 이하5 or less 불검출Non-detection 수은(㎎/㎏)Mercury (mg / kg) 2 이하2 or less 불검출Non-detection 납(㎎/㎏)Lead (mg / kg) 130 이하130 or less 불검출Non-detection 크롬(㎎/㎏)Chromium (mg / kg) 250 이하250 or less 28.7828.78 구리(㎎/㎏)Copper (mg / kg) 400 이하400 or less 12.3912.39 니켈(㎎/㎏)Nickel (mg / kg) 45 이하45 or less 5.645.64 아연(㎎/㎏)Zinc (mg / kg) 1000 이하1000 or less 37.0037.00 염분(%)salt(%) 2.0 이하2.0 or less 1.811.81 부숙도Compost 4 이상4 or more 66 염기치환용량(Cmol/Kg)Base replacement capacity (Cmol / Kg) 13.5013.50 대장균O157:H7E. coli O157: H7 불검출Non-detection 불검출Non-detection 살모넬라Salmonella 불검출Non-detection 불검출Non-detection PH[1:10]PH [1:10] 5.155.15 전기전도도[1:10](mS/cm)Electrical Conductivity [1:10] (mS / cm) 6.266.26

Claims (4)

바실러스 스미시(Bacillus smithii) KCTC 3618 및 바실러스 스미시(Bacillus smithii) ATCC 55404를 유기성 폐기물에 접종하고 40 내지 80℃에서 발효시키는 것을 특징으로 하는 유기성 폐기물 처리방법.Bacillus Sumi when (Bacillus smithii) KCTC 3618 and Bacillus Sumi when (Bacillus smithii) organic waste treatment method, comprising a step of inoculation the ATCC 55404 in the organic waste, and fermentation at 40 to 80 ℃. 바실러스 스미시(Bacillus smithii) KCTC 3618 및 바실러스 스미시(Bacillus smithii) ATCC 55404를 포함하는 유기성 폐기물 처리용 미생물 제제. Bacillus smithii KCTC 3618 and Bacillus smithii ATCC 55404 for the treatment of organic waste. 바실러스 스미시(Bacillus smithii) KCTC 3618 및 바실러스 스미시(Bacillus smithii) ATCC 55404를 배지에 접종하고 50 내지 80℃에서 배양하여 배양액을 수득하는 단계; 및
톱밥 또는 쌀겨에 상기 배양액을 첨가하고 50 내지 80℃에서 교반하는 단계;를 포함하는 유기성 폐기물 처리용 미생물 제제 제조방법. Bacillus smithii KCTC 3618 and Bacillus smithii ATCC 55404 are inoculated in a medium and cultured at 50 to 80 캜 to obtain a culture solution; And
Adding the culture medium to sawdust or rice bran, and stirring the mixture at 50 to 80 캜. 제 3항의 제조방법으로 제조된 유기성 폐기물 처리용 미생물 제제.
A microorganism preparation for treating organic waste produced by the method of claim 3.
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