KR101698018B1 - Extracting device of regenerative cells for medical - Google Patents

Extracting device of regenerative cells for medical Download PDF

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Publication number
KR101698018B1
KR101698018B1 KR1020150124218A KR20150124218A KR101698018B1 KR 101698018 B1 KR101698018 B1 KR 101698018B1 KR 1020150124218 A KR1020150124218 A KR 1020150124218A KR 20150124218 A KR20150124218 A KR 20150124218A KR 101698018 B1 KR101698018 B1 KR 101698018B1
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liquid
transfer
transfer tube
water
sensor
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KR1020150124218A
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Korean (ko)
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김태영
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주식회사 베스테크
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/26—Inoculator or sampler
    • B01F13/00—
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B5/00—Other centrifuges
    • B04B5/10—Centrifuges combined with other apparatus, e.g. electrostatic separators; Sets or systems of several centrifuges
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B7/00—Elements of centrifuges
    • B04B7/02—Casings; Lids
    • B04B7/06—Safety devices ; Regulating
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/02—Apparatus for enzymology or microbiology with agitation means; with heat exchange means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/10—Apparatus for enzymology or microbiology rotatably mounted
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/069—Vascular Endothelial cells
    • C12N5/0692—Stem cells; Progenitor cells; Precursor cells
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
    • F16L—PIPES; JOINTS OR FITTINGS FOR PIPES; SUPPORTS FOR PIPES, CABLES OR PROTECTIVE TUBING; MEANS FOR THERMAL INSULATION IN GENERAL
    • F16L55/00—Devices or appurtenances for use in, or in connection with, pipes or pipe systems
    • B01F2215/0073—

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  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Sustainable Development (AREA)
  • Cell Biology (AREA)
  • Vascular Medicine (AREA)
  • Developmental Biology & Embryology (AREA)
  • Mechanical Engineering (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The present invention relates to a medical regenerative cell extracting apparatus, and more particularly, to a medical regenerative cell extracting apparatus capable of rapidly separating and extracting regenerated cells from adipose tissue by forming a chamber for washing adipose tissue and a chamber for extracting regenerated cells But also to facilitate the washing, separation and extraction of regenerative cells automatically and easily.
The present invention relates to an apparatus for separating and extracting regenerative cells from adipose tissue,
An extractor body driven to extract regenerative cells; A mixing chamber for mixing the fat tissue and the solution; A centrifugal separator for washing and separating the regenerative cells; A water chamber for storing the water for injection; A transfer pipe connected to transfer the extract separated from the water; A peristaltic pump that pressurizes the liquid in the transfer tube to transfer the liquid; A pinch valve for controlling the liquid to be transferred; A controller for controlling driving through a sensing signal of each sensor; . ≪ / RTI >

Description

Extracting device for regenerative cells for medical use

The present invention relates to an apparatus for separating and extracting regenerative cells from adipose tissue. More particularly, the present invention relates to an apparatus for separating and extracting regenerative cells from adipose tissue, And the washing, separation and extraction of regenerative cells can be carried out automatically and conveniently.

Generally, the fat cells (PLA: Processed Lipo Aspirate) obtained through the fat extraction process include fat cells and many stem cells, and thus utilize surplus fat.

In the past, adipose tissue was regarded only as a caloric storage structure that stores surplus energy, and it was regarded as an object to be removed only. Recently, as a result of development of cell culture technology and cell purification technology, a large amount of adult stem cells Regenerated cells (PLA Cells - Processed Lipo Aspirate Cells) have been found to exist.

These regenerated cells are composed of adult stem cells, endothelial progenitor cells, growth factor producing cells, etc. and can be easily used for various treatments without special operations such as culture .

In particular, a large number of adult stem cells in adipose tissue are expected to play a crucial role in treating various intractable diseases and serious diseases as well as performing cosmetic or reconstructive functions required in plastic surgery field.

Therefore, in the past, various separation and extraction systems have been studied for separating and extracting the regenerated cells. In recent years, a centrifugal separation apparatus for stem cells has been developed and used in cosmetic molding and therapeutic drug development.

The conventional apparatus for separating and extracting regenerative cells comprises an apparatus main body driven mainly to extract stem cells, a centrifugal separator for separating the stem cells, a transfer unit connected to transfer the separated liquid, A peristaltic pump for transferring the liquid in the transfer tube, and a separation unit for sensing, controlling, transporting and sorting the transfer of the liquid, and separating and extracting the stem cell layer.

As a conventional prior art document related to such a regenerative cell extracting system, there is a system and a method for separating and concentrating regenerative cells from tissue, which is called Korean Patent Application Publication No. 10-2006-0036861.

The above-mentioned conventional invention provides an automated system and method for separating and concentrating regenerating cells, for example, stem cells and progenitor cells, from adipose tissue.

Another conventional prior art document is the 'regenerative cell extraction system' of the Korean Patent Publication No. 10-2011-0045479.

The above-mentioned conventional invention is completely automated by the program in a state where the process from the collection of the adipose tissue to the processing of the tissue is closed, the purity of the regenerating cells collected from the adipose tissue is improved, and finally, And the need for separate manipulation after extraction of the cells is reduced.

However, the above-described conventional separation and extraction apparatus for regenerating cells has a limitation in the separation and extraction method depending on the use because it separates and extracts the regenerating cells from adipose tissue by the centrifugal separation method, The research and development of the system was necessary.

In addition, in the process of centrifuging the adipose tissue of the regenerating cells, it is difficult to separate the active ingredient from the adipose tissue, resulting in an extremely inefficient cell, resulting in a problem that the separation and extraction process takes a long time.

In order to solve the above-described problems, the present invention provides a method of separating and extracting regenerated cells from adipose tissue by forming a chamber for washing adipose tissue and a chamber for extracting regenerated cells, So that the cleaning, separation and extraction processes can be automatically and easily carried out.

The present invention relates to an apparatus for separating and extracting regenerative cells from adipose tissue,

An extractor body driven to extract regenerative cells; A mixing chamber for mixing the fat tissue and the solution; A centrifugal separator for washing and separating the regenerative cells; A water chamber for storing the water for injection; A transfer pipe connected to transfer the extract separated from the water; A peristaltic pump that pressurizes the liquid in the transfer tube to transfer the liquid; A pinch valve for controlling the liquid to be transferred; A controller for controlling driving through a sensing signal of each sensor; . ≪ / RTI >

As described above, according to the present invention, by forming a chamber for washing adipose tissue and a chamber for extracting regenerated cells, not only the process of separating and extracting regenerated cells from adipose tissue can be performed more quickly, Separating and extracting process can be automatically and easily carried out.

FIG. 1 is a perspective view showing a medical regeneration cell extracting apparatus of the present invention, FIG. 2 is a perspective view showing a use state of the present invention, FIG. 3 is a perspective view showing a mixing chamber of the present invention, 6 is a perspective view showing the water chamber of the present invention, Fig. 7 is a perspective view showing the peristaltic pump of the present invention, Fig. 8 is a perspective view showing the operation of the centrifugal separator of the present invention, FIG. 9 is a perspective view of a control unit of the present invention. FIG.
In the drawings, the same reference numerals are used to designate the same or similar components throughout the drawings.
In the following description of the present invention, a detailed description of known functions and configurations incorporated herein will be omitted when it may make the subject matter of the present invention rather unclear.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, the configuration and effects of the present invention will be described in detail with reference to the accompanying drawings.

As shown in Figs. 1 to 9, the present invention comprises an extractor body 10 driven to extract regenerative cells, a mixing chamber 20 for mixing the fat tissue and the solution, A centrifugal separator (30) for centrifugal separation, a water chamber (40) for storing the water for injection, a transfer tube (50) connected to transfer the extract separated from the water, and a peristaltic pump A pinch valve 70 for controlling the liquid to be transferred, and a control unit 80 for controlling the driving through the sensing signal of each sensor.

The extractor body 10 includes a touch screen unit 11 for inputting a regeneration cell extraction process and displaying an operation state, a syringe holder 12 for receiving a syringe, The processing liquid collection box 13 is formed.
The syringe holder 12 fixes and mounts a syringe for connecting and separating the distal end of the delivery tube to collect the regenerated cells, and removes the syringe.

The mixing chamber 20 includes a lid 21 for opening and closing the upper part, a container 22 for introducing fat tissue and solution, a unit 23 for rotating the container 22 inwardly, A fixed shaft portion 24 fixed to one side of the unit 23, a turning shaft portion 25 fastened to the lower portion of the unit 23, And a lid sensor 27 for sensing the opening and closing of the lid 21 are formed.

The fixed shaft portion 24 defines a fixture 24a fixed to one side of the unit and a fixed shaft 24b penetrated through the fixture.

The rotation shaft portion 25 includes a rotation shaft 25a rotated by a motor and a cam 25b connected to the rotation shaft 25a and one end connected to the cam 25b and the other end connected to the unit 23 And a link 25c connected to a lower portion of the link 25c.

The centrifugal separator 30 includes an opening / closing door 31 for automatically opening / closing an upper portion of the centrifugal separator 30, a housing 32 for rotating the centrifugal separator 30 into which the fat cells have been inserted, A motor 33, an opening / closing door sensor 34 for detecting whether the opening / closing door 31 is open or closed, a water leakage sensor 35 for detecting the leakage of the liquid inside the door 33, Thereby forming a door motor 36 for driving.

The water chamber 40 has a cradle 41 for receiving the water for injection, a heat plate 42 for heating the internal temperature to keep the internal temperature constant, and a temperature sensor 43 for sensing the internal temperature .

The conveyance pipe portion 50 includes a conveyance pipe 51 connected to convey the extracted liquid separated from the injection water, a pressure sensor 52 for sensing the pressure in the conveyance pipe, And an air filter valve 54 for introducing outside air into the transfer pipe to remove the negative pressure in the pipe are formed.

The peristaltic pump 60 is provided with a moving means 61 for automatically moving the transfer tube 51 to fix the transfer tube 51 and a fixed transfer tube 51 for rotating the transfer tube 51, A pump motor 63 for rotating the roller 62, a moving ball motor 64 for driving the moving ball 61 to reciprocate forward and backward, And a conversion unit 65 for converting the rotational force of the spherical motor into a linear reciprocating motion is formed.

The roller 62 is rotated in the forward and reverse directions by the pump motor 63 so as to move the liquid inside the transfer pipe in both directions.

The pinch valve 70 is provided with a fixing groove 71 for controlling the movement of the liquid in the transfer tube 51 and for fixing the transfer tube, a solenoid portion 72 for being operated by a control signal, A shutoff port 73 is formed which moves up and down by the operation of the solenoid portion 72 to shut off and control the transfer of the liquid in the transfer pipe.

The control unit 80 is configured to receive a signal sensed by each sensor and transmit a control signal according to the sensed signal, and further includes a sensing sensor 81 provided on the side of the peristaltic pump so as to sense a component of the object conveyed in the conveyance pipe, A pressure sensor 82 for detecting the pressure in the transfer pipe, and an air filter valve 83 for introducing outside air into the transfer pipe to remove the negative pressure in the pipe.

BRIEF DESCRIPTION OF THE DRAWINGS The above and other objects, features and advantages of the present invention will be more apparent from the following detailed description taken in conjunction with the accompanying drawings, in which: FIG.

As shown in Figs. 1 to 9, in order to extract regenerative cells such as adipose stem cells from adipose tissue, the user opens the lid 21 of the mixing chamber 20 of the regenerative cell extracting apparatus, 22).

When the user inputs the mode and the mode of use by using the touch screen unit 11 of the extractor body 10 in consideration of the amount of fat and the like, The stored water, such as a saline pack, is transferred through the transfer tube 51 and injected into the container 22 of the mixing chamber 20.

6, the water such as a saline pack is kept at a constant temperature and is injected into the container 22 by the heat plate 42 and the temperature sensor 43 inside the water chamber 40 .

3 and 4, the unit 23 of the mixing chamber 20 into which the container 22 is inserted is rotated by the fixed shaft portion 24 and the rotating shaft portion 25, The unit 23 is vertically rotated in the half-hoop shape. By rotating the unit 23, the fatty tissue and the water in the container 22 are mixed and washed, and the generated wastewater is transferred to the transfer tube 51 To the processing liquid collecting box 13 through the inlet port 13a.

At this time, the temperature in the mixing chamber 20 can be kept constant by the hot pad 26, and the opening of the lid 21 during the rotation operation of the unit 23 is sensed through the lid sensor 27, It can prevent safety accidents.

5, when the enzymatic treatment is completed after the washing in the mixing chamber 20, the peristaltic pump 60 is operated to move the cell fluid in the mixing chamber 20 to the centrifuge vessel of the centrifugal separator 30 .

The housing 32 in which the centrifuge vessel is inserted is rotated by the motor 33 to separate the cells and waste fluid by the centrifugal force and the separated regenerated cells are transferred to the syringe holder 12 through the transfer tube 51, The residual liquid in the transfer pipe 51 is checked through the pressure sensor 52 and the peristaltic pump 60 is re-operated to transfer the remaining liquid in the transfer pipe 51 to the syringe connected to the end of the transfer pipe, Can be transferred completely.

At this time, opening and leakage of the opening / closing door 31 can be sensed by the opening / closing door sensor 34 and the water leakage sensor 35 of the centrifuge 30, so that the extraction operation can be carried out safely. Is automatically rotated by the door motor 36 to be opened and closed.

7 and 8, when the liquid in the transfer pipe 50 is moved and moved by the peristaltic pump 60, the rotation of the moving sphere motor 64 and the rotation of the transfer unit 50 by the conversion unit 65, The transfer port 61 is moved back and forth so that the transfer tube 50 is brought into close contact with the roller 62 and fixed.

As the roller 62 rotates through the rotation of the pump motor 63, the transfer tube 51 inserted into the moving tool 61 is continuously pressed and pressed by the rotation of the roller 62, The liquid in the container 51 is smoothly transported.

The sensing unit 53 of the conveying pipe unit 50 senses the components in the conveying pipe and controls the conveying and blocking of the conveying pipe by the control unit 80. The pressure sensor 52 senses and confirms the pressure in the conveying pipe, The air filter valve 54 is opened to allow outside air to be introduced, so that the negative pressure and the residual pressure in the transfer pipe can be removed.

8, the shutoff port 73 is raised and lowered by the operation of the solenoid portion 72 in the conveyance pipe 51 which is fixed and fixed to the fixing groove 71 of the pinch valve 70, The flow path of the liquid in the transfer pipe 51 is shut off and controlled so that the transfer path of each path and the section can be controlled. In each process, separation, extraction, and mixing of the transferred components are blocked You can.

As shown in FIG. 9, the controller 80 receives signals such as temperature and pressure sensed by the respective sensors. The control unit 80 controls the respective driving means .

The foregoing description is merely illustrative of the technical idea of the present invention, and various changes and modifications may be made by those skilled in the art without departing from the essential characteristics of the present invention.

Therefore, the embodiments disclosed in the present invention are intended to illustrate rather than limit the scope of the present invention, and the scope of the technical idea of the present invention is not limited by these embodiments.

The scope of protection of the present invention should be construed according to the following claims, and all technical ideas within the scope of equivalents should be construed as falling within the scope of the present invention.

1 is a perspective view showing a medical regenerative cell extracting apparatus of the present invention;
2 is a perspective view showing a use state of the present invention;
Figure 3 is a perspective view of the mixing chamber of the present invention.
4 is a perspective view showing an operation state of the mixing chamber of the present invention;
5 is a perspective view showing the centrifugal separator of the present invention
6 is a perspective view showing the water chamber of the present invention.
7 is a perspective view showing the peristaltic pump of the present invention
8 is a perspective view showing the pinch valve of the present invention.
9 is a block diagram of a control unit of the present invention
DESCRIPTION OF THE REFERENCE SYMBOLS
10: extractor main body 11: touch screen part
12: Syringe holder 13: Treatment liquid collection box
20: mixing chamber 21: cover
22: container 23: unit
24: fixed shaft portion 24a: fastener
24b: fixed shaft 25: rotary shaft portion
25a: rotation shaft 25b: cam
25c: Link 26: Thermal pad
27: Cover sensor 30: Centrifuge
31: opening / closing door 32: housing
33: motor 34: opening / closing door sensor
35: leak sensor 36: door motor
40: water chamber 41: cradle
42: heat plate 43: temperature sensor
50: feed pipe 51: feed pipe
52: pressure sensor 53: detection sensor
54: air filter valve 60: peristaltic pump
61: moving part 62: roller
63: Pump motor 64: Moving sphere motor
65: conversion section 70: pinch valve
71: Fixing groove 72: Solenoid part
73: shutoff port 80:

Claims (9)

An extractor body 10 driven to extract regenerative cells;
A mixing chamber (20) for mixing the fat tissue and the solution;
A centrifuge (30) for washing and separating the regenerative cells;
A water chamber (40) for storing the injection water;
A transfer tube (50) connected to transfer the extract separated from the water;
A peristaltic pump (60) which pressurizes the liquid in the transfer tube to transfer the liquid;
A pinch valve (70) for controlling the liquid to be transferred;
A control unit (80) for controlling driving through a sensing signal of each sensor;
An apparatus for separating and extracting regenerative cells from adipose tissue,
The extractor body 10 includes a touch screen unit 11 for inputting a regeneration cell extraction process and displaying an operation state, a syringe holder 12 for receiving a syringe, A processing liquid collection box 13 is formed;
The mixing chamber 20 includes a lid 21 for opening and closing the upper part, a container 22 for introducing the fat tissue and solution, a unit 23 for rotating the container 22 inwardly, A fixed shaft portion 24 fixed to one side surface of the unit 23, a rotating shaft portion 25 fastened to the lower portion of the unit 23, And a lid sensor (27) for sensing opening and closing of the lid (21);
The centrifugal separator 30 includes an opening and closing door 31 for automatically opening and closing an upper portion of the centrifugal separator 30, a housing 32 for rotating the centrifugal separator into which the adipocytes are introduced and rotating the centrifugal separator 30, An opening / closing door sensor 34 for detecting whether the opening / closing door 31 is open or closed, a water leakage sensor 35 for detecting a leakage of the solution in the inside, A door motor (36) is formed.
The water chamber 40 has a holder 41 for holding the water for injection, a heat plate 42 for heating the internal temperature to be constant, and a temperature sensor 43 for sensing the internal temperature;
The conveyance pipe portion 50 includes a conveyance pipe 51 connected to convey the extracted liquid separated from the injection water, a pressure sensor 52 for sensing the pressure in the conveyance pipe, A detection sensor 53, and an air filter valve 54 for introducing outside air into the transfer pipe to remove the negative pressure in the pipe;
The peristaltic pump 60 is provided with a moving port 61 that moves automatically to fix the transfer tube 51 and a fixed transfer tube 51 which are rotated by the rotation so as to move the liquid inside the transfer tube A pump motor 63 for rotating the roller 62 and a movable sphere motor 64 for driving the sphere 61 to reciprocate forward and backward, , And a conversion section (65) for converting the rotational force of the moving orifice motor into a linear reciprocating motion is formed;
The pinch valve 70 is provided with a fixing groove 71 for controlling the movement of the liquid in the transfer tube 51 and for fixing the transfer tube, a solenoid portion 72 for being operated by a control signal, Is configured to form a blocking port (73) for vertically moving by the operation of the solenoid section (72) to block and control the transfer of the liquid in the transfer tube.
delete delete The method according to claim 1,
The fixed shaft portion 24 of the mixing chamber 20 has a fixing hole 24a fixed to one side of the unit 23 and a fixing shaft 24b penetrating the fixing hole 24a,
The rotation shaft portion 25 includes a rotation shaft 25a rotated by a motor and a cam 25b connected to the rotation shaft 25a and one end connected to the cam 25b and the other end connected to the unit 23, And a link (25c) connected to a lower portion of the cell.
delete delete delete delete delete
KR1020150124218A 2015-09-02 2015-09-02 Extracting device of regenerative cells for medical KR101698018B1 (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20180113405A (en) * 2017-04-06 2018-10-16 김태영 Kit for extracting device of regenerative cells

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20060030861A (en) * 2003-06-25 2006-04-11 마크로포어 바이오서저리, 인크. Systems and methods for separating and concentrating regenerative cells from tissue
JP2012055227A (en) * 2010-09-08 2012-03-22 Olympus Corp Cell treatment device
KR20130068203A (en) * 2011-12-14 2013-06-26 도병록 System and method for extracting regenerative cells
KR101354959B1 (en) * 2013-07-09 2014-01-22 김태영 A separator for stem cell

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20060030861A (en) * 2003-06-25 2006-04-11 마크로포어 바이오서저리, 인크. Systems and methods for separating and concentrating regenerative cells from tissue
JP2012055227A (en) * 2010-09-08 2012-03-22 Olympus Corp Cell treatment device
KR20130068203A (en) * 2011-12-14 2013-06-26 도병록 System and method for extracting regenerative cells
KR101354959B1 (en) * 2013-07-09 2014-01-22 김태영 A separator for stem cell

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20180113405A (en) * 2017-04-06 2018-10-16 김태영 Kit for extracting device of regenerative cells
KR101940773B1 (en) * 2017-04-06 2019-01-21 김태영 Kit for extracting device of regenerative cells

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