KR101693283B1 - Nonylphenol responsive genes in Oryzias javanicus and the method for diagnosing aquatic environment pollution using the same - Google Patents

Nonylphenol responsive genes in Oryzias javanicus and the method for diagnosing aquatic environment pollution using the same Download PDF

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KR101693283B1
KR101693283B1 KR1020160070839A KR20160070839A KR101693283B1 KR 101693283 B1 KR101693283 B1 KR 101693283B1 KR 1020160070839 A KR1020160070839 A KR 1020160070839A KR 20160070839 A KR20160070839 A KR 20160070839A KR 101693283 B1 KR101693283 B1 KR 101693283B1
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염승식
조예진
우선옥
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한국해양과학기술원
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Abstract

The present invention relates to an Oryzias javanicus gene responsive to nonylphenol (NP) exposure, and to a method for diagnosing aquatic environmental contamination by using the same. In particular, Oryzias javanicus is cultured, and is exposed to nonylphenol at a concentration of 20 g/L for 12 hours, 24 hours, 48 hours, and 72 hours, and RNA is separated therefrom. Then, cDNA is synthesized and marked by Cy3 and Cy5 before performing hybridization. An oligo-microarray is produced and analyzed by utilizing an EnviHaz-Fish array. As a result, it has been confirmed that an expression amount of 123 genes is changed by twice or more compared to a control group by the exposure of nonylphenol, using heterogeneous microarray experimental analysis. Accordingly, the genes can be useful as biomarkers for confirming nonylphenol exposure.

Description

노닐페놀 노출에 대응하는 바다송사리 유전자 및 이를 이용한 수생태계 환경오염 진단 방법{Nonylphenol responsive genes in Oryzias javanicus and the method for diagnosing aquatic environment pollution using the same}[0001] Description [0002] The present invention relates to a method for diagnosing environmental pollution of a marine ecosystem using a sea otter gene corresponding to exposure to nonylphenol,

본 발명은 노닐페놀(Nonylphenol, NP) 노출에 대응하는 바다송사리(Oryzias javanicus) 유전자 및 이를 이용하여 수생태계 환경오염을 진단하는 방법 에 관한 것이다.The invention sea medaka (Oryzias corresponding to nonylphenol (Nonylphenol, NP) exposure javanicus gene and a method for diagnosing environmental pollution of aquatic ecosystem using the same.

노닐페놀(Nonylphenol, NP)은 계면활성제로써 세척제나 세정제, 섬유유연제 등에 이용되며, 에폭시수지 및 페인트 첨가제, 동박적층판, 잉크바인더, 농약 등에 사용되고 있다. 환경으로 배출된 NP는 어류를 포함한 수생생물들의 내분비계를 교란시키는 등의 강한 독성효과를 나타낸다. 또한 생분해되지 않고 생태계 내에 장기간 남아 생태계의 먹이망을 통해 생물 농축됨으로써 전 지구적인 문제를 야기시킨다. 인체의 경우 NP에 노출되면 소화계, 눈, 신장, 간, 생식계, 피부 및 인두 등에 악영향을 주는 것으로 알려져 있다. 현재 공중위생관리법에 근거하여 주방용 세척제로의 사용 및 식품 첨가물로의 사용이 금지되어 있고, 유럽에서는 세척제나 화장품등에 사용하는 것도 금지되어 있다.Nonylphenol (NP) is a surfactant used in detergents, cleaners, fabric softeners, etc., and is used in epoxy resins and paint additives, copper clad laminates, ink binders, pesticides and the like. NP released into the environment shows a strong toxic effect such as disturbing the endocrine system of aquatic organisms including fish. It is also biodegraded and remains in the ecosystem for a long time, resulting in global problems by bioconcentration through the food web of the ecosystem. In the human body, exposure to NPs has been known to adversely affect the digestive system, eyes, kidneys, liver, reproductive system, skin and pharynx. It is currently prohibited to use it as a dishwashing detergent or as a food additive based on the Public Hygiene Control Act. In Europe, it is prohibited to use it as a cleaning agent or cosmetics.

생물들은 환경오염, 기후변화 및 병원성 미생물의 감염 등과 같은 외부 환경변화에 대응하여, 항상성을 유지하기 위해 생체방어기작을 진화시켜왔다. 이 생체방어기작의 대부분은 특정 유전자의 발현량을 능동적으로 변화시켜, 특정 단백질의 양을 조절하는 것으로 나타난다. 따라서 특정 변화에 대한 특이적인 유전자들을 발굴하여 이들의 발현량 변화를 모니터링한다면, 특정지역의 환경변화에 관한 정보뿐만 아니라, 이러한 환경변화가 생명현상에 미치는 영향 및 생태계의 건강에 관한 정보를 얻을 수 있다. Biologists have evolved biological defense mechanisms to maintain homeostasis in response to changes in the external environment, such as environmental pollution, climate change, and infection with pathogenic microorganisms. Most of these mechanisms of defense appear to modulate the amount of a specific protein by actively changing the expression level of a particular gene. Therefore, if specific genes for specific changes are identified and monitored for changes in their expression levels, information on environmental changes in specific areas as well as information on the effects of these environmental changes on life events and ecosystem health can be obtained have.

이에, 본 발명자들은 바다송사리를 대상으로 환경오염에 대응하는 생체지표의 발굴을 시도하고 있으며, 노닐페놀 노출에 대한 특이 유전자 후보의 확보 및 검출을 위해, 송사리와 같은 속에 속하는 바다송사리(Oryzias javanicus) 유전자 앤비헤즈 피쉬 배열(EnviHaz-Fish Array)을 활용한, 마이크로어래이 실험분석을 통해, 노닐페놀 노출에 123종의 유전자 발현량이 대조군 대비 2배이상 증가 또는 감소하는 것을 확인함으로써, 이들 유전자들은 노닐페놀 노출을 확인할 수 있는 바이오마커로 활용하여, 노닐페놀 노출 여부를 확인할 수 있는 어류 생체지표 및 이들 유전자 생체지표들의 발현량 변화를 규명함으로써 본 발명을 완성하였다.Therefore, the present inventors have attempted to find a biomarker corresponding to environmental pollution of sea urchins, and in order to obtain and detect a specific gene candidate for exposure to nonylphenol, Oryzias javanicus belonging to the genus such as urchins , Microarray experiments using the EnviHaz-Fish Array showed that the amount of 123 genes expressed in the nonylphenol exposure increased or decreased by more than 2-fold compared to the control group, The present invention has been accomplished by identifying changes in the expression level of fish biomarkers and non-exogenous biomarkers that can confirm exposure to nonylphenol.

본 발명의 목적은 노닐페놀(Nonylphenol, NS) 노출에 대응하여 발현량이 변화된 바다송사리(Oryzias javanicus) 유전자 및 이를 이용한 노닐페놀 노출 여부를 확인하는 방법을 제공하는 것이다.An object of the present invention is nonylphenol (Nonylphenol, NS) to a changed amount of expression sea medaka corresponding to exposure (Oryzias javanicus gene and a method for confirming whether or not it is exposed to nonylphenol.

상기 목적을 달성하기 위하여, 본 발명은 서열번호 1 내지 123으로 기재되는 모든 유전자 각각의 핵산 서열의 올리고뉴클레오티드 전부 또는 그의 상보가닥 분자가 집적된, 시료내 노닐페놀(Nonylphenol, NP) 노출 여부 검출용 마이크로어래이 칩(microarray chip)을 제공한다. In order to accomplish the above object, the present invention provides a method for detecting the presence or absence of nonylphenol (NP) in a sample in which oligonucleotides of all the nucleotide sequences of all the genes of SEQ ID NOS: 1 to 123, And provides a microarray chip.

또한, 본 발명은In addition,

1) 피검 시료에 노출된 실험군의 바다송사리와, 정상 대조군의 바다송사리에서 각각 RNA를 분리하는 단계;1) separating the RNA from the sea cucumber of the test group exposed to the test sample and the sea cucumber of the normal control group, respectively;

2) 단계 1)의 실험군 및 대조군의 RNA로부터 cDNA를 합성하면서 실험군과 대조군을 각기 다른 형광물질로 표지하는 단계;2) labeling the experimental group and the control group with different fluorescent substances while synthesizing cDNA from the RNA of the experimental group and the control group of step 1);

3) 단계 2)의 각기 다른 형광물질로 표지된 cDNA를 제 1항의 마이크로어래이 칩과 혼성화시키는 단계;3) hybridizing the cDNA labeled with different fluorescent substances of step 2) with the microarray chip of claim 1;

4) 반응한 마이크로어래이 칩을 분석하는 단계; 및4) analyzing the reacted microarray chip; And

5) 분석한 데이터에서 제 1항의 마이크로어래이 칩에 집적된 유전자 발현 정도를 대조군과 비교하여 확인하는 단계를 포함하는, 시료내 노닐페놀 노출 여부 검출 방법을 제공한다. 5) A method for detecting the presence or absence of nonylphenol in a sample, comprising the step of comparing the degree of gene expression integrated in the microarray chip of claim 1 with the control group.

또한 본 발명은, Further, according to the present invention,

1) 피검시료에 노출된 실험군의 바다송사리(Oryzias javanicus)와, 정상 대조군의 바다송사리에서 각각 RNA를 분리하는 단계;1) separating the RNA from the Oryzias javanicus of the test group exposed to the test sample and the sea lion of the normal control group, respectively;

2) 단계 1)의 RNA를, 서열번호 1 내지 123으로 기재되는 각각의 유전자에 상보적이고 유전자를 증폭할 수 있는 프라이머 쌍을 모두 사용하여 정량 실시간 RT-PCR(Quantitative real-time reverse transcript polymerase chain reaction, qRT-PCR)을 수행하는 단계; 및2) The RNA of step 1) was subjected to quantitative real-time reverse transcriptase polymerase chain reaction (RT-PCR) using both primer pairs complementary to each gene described in SEQ ID NOS: , qRT-PCR); And

3) 단계 2)의 유전자 산물을 대조군과 비교하여 발현 정도를 확인하는 단계를 포함하는, 시료내 노닐페놀 노출 여부 검출 방법을 제공한다.3) comparing the gene product of step 2) with a control group to confirm the degree of expression, and detecting the presence or absence of nonylphenol in the sample.

또한, 본 발명은 상기 마이크로어래이 칩을 포함하는 노닐페놀 노출 여부 검출용 키트를 제공한다. The present invention also provides a kit for detecting whether or not to expose nonylphenol containing the microarray chip.

아울러, 본 발명은 서열번호 1 내지 123으로 기재되는 각각의 유전자에 상보적이고 유전자를 증폭할 수 있는 프라이머 쌍을 모두 포함하는 노닐페놀 노출 여부 검출용 키트를 제공한다. In addition, the present invention provides a kit for detecting exposure to nonylphenol comprising all of the primer pairs complementary to each gene described in SEQ ID NOS: 1-123 and capable of amplifying a gene.

본 발명에서 송사리와 같은 속에 속하는 바다송사리(Oryzias javanicus) 유전자 앤비헤즈 피쉬 배열(EnviHaz-Fish Array)를 활용한, 이종간의 마이크로어래이 실험분석을 통해, 노닐페놀 12, 24, 48 또는 72시간 동안의 노출에 의해 123종의 유전자 발현량이 대조군 대비 2배이상 증가 또는 감소하는 것을 확인함으로써, 이들 유전자들은 노닐페놀 노출여부를 확인할 수 있는 바이오마커로 유용하게 사용될 수 있다. In the present invention, Oryzias javanicus) gene aenbi heads fish arrangement (EnviHaz-Fish Array) to utilize one, through a micro eoraeyi experimental analysis of the heterogeneous, control the amount of gene expression of the 123 species by exposure of nonylphenol 12, for 24, 48 or 72 hours against 2 By confirming that these genes increase or decrease more than twice, these genes can be usefully used as biomarkers for confirming whether or not nonylphenol is exposed.

이하 본 발명을 상세히 설명한다. Hereinafter, the present invention will be described in detail.

본 발명은 노닐페놀(nonylphenol, NP) 노출에 대응하여 발현이 변화하는 바다송사리(Oryzias javanicus) 유래의 유전자를 발굴하여, 상기 노닐페놀 노출에 대하여 발현량이 변화하는 바다송사리 유래 유전자를 집적한 마이크로어래이 칩을 노닐페놀 노출 여부 검출 및 수생태계 오염 상태를 진단하는데 이용할 수 있다.The present invention relates to a microarray in which a gene derived from Oryzias javanicus whose expression is changed in response to exposure to nonylphenol (NP) is digested, and a gene derived from marine callus, whose expression amount changes with respect to exposure to nonylphenol, The chip can be used to detect nonylphenol exposure and to diagnose aquatic ecosystem contamination.

본 발명은 1 내지 123으로 기재되는 모든 유전자 각각의 핵산 서열의 올리고뉴클레오티드 전부 또는 그의 상보가닥 분자가 집적된, 시료내 노닐페놀(Nonylphenol, NP) 노출 여부 검출용 마이크로어래이 칩(microarray chip)을 제공한다.The present invention provides a microarray chip for detecting the presence or absence of nonylphenol (NP) in a sample in which oligonucleotides of all nucleic acid sequences of all the genes 1 to 123 or their complementary strand molecules are integrated do.

상기 유전자는 바다송사리(Oryzias javanicus)로부터 유래된 것이 바람직 하다. The gene is preferably derived from Oryzias javanicus .

또한 상기 상기 마이크로어래이는 EnviHaz-Fish 마이크로어래이인 것이 바람직 하나 이에 한정하지 않는다. The micro-array may be an EnviHaz-Fish microarray, but is not limited thereto.

상기 노닐페놀 노출 여부 검출용 마이크로어래이 칩은 당업자에게 알려진 방법으로 제작할 수 있다. 상기 마이크로어래이 칩을 제작하는 방법은 하기와 같다. 상기 탐색된 유전자를 프로브로 이용하여 마이크로어래이 칩의 기판상에 고정화시키기 위해 파이조일렉트릭(piezoelectric) 방식을 이용한 마이크로피펫팅(micropipetting)법 또는 핀(pin) 형태의 스폿터(spotter)를 이용한 방법 등을 사용하는 것이 바람직하나 이에 한정되지 않는다. 상기 마이크로어래이 칩의 기판은 아미노-실란(amino-silane), 폴리-L-라이신(poly-Llysine) 및 알데히드(aldehyde)로 이루어진 군에서 선택되는 하나의 활성기가 코팅된 것이 바람직하나 이에 한정되지 않는다. 또한, 상기 기판은 슬라이드 글라스, 플라스틱, 금속, 실리콘, 나일론 막, 및 니트로셀룰로스 막으로 이루어진 군에서 선택될 수 있으나 이에 한정되지 않는다.The microarray chip for detecting the presence or absence of nonylphenol can be produced by a method known to a person skilled in the art. A method of manufacturing the microarray chip is as follows. A micropipetting method using a piezo electric method or a method using a pin type spotter to immobilize the searched gene on a substrate of a microarray chip using the probe as a probe But it is not limited thereto. The substrate of the microarray chip is preferably coated with one activator selected from the group consisting of amino-silane, poly-L-lysine and aldehyde, but is not limited thereto . The substrate may be selected from the group consisting of slide glass, plastic, metal, silicon, nylon film, and nitrocellulose film, but is not limited thereto.

또한, 본 발명은 1) 피검 시료에 노출된 실험군의 바다송사리와, 정상 대조군의 바다송사리에서 각각 RNA를 분리하는 단계;The present invention also relates to a method of isolating a test sample from a test sample, comprising the steps of: (1) separating RNA from an offspring of an experimental group exposed to a test sample and an offspring of a normal control group;

2) 단계 1)의 실험군 및 대조군의 RNA로부터 cDNA를 합성하면서 실험군과 대조군을 각기 다른 형광물질로 표지하는 단계;2) labeling the experimental group and the control group with different fluorescent substances while synthesizing cDNA from the RNA of the experimental group and the control group of step 1);

3) 단계 2)의 각기 다른 형광물질로 표지된 cDNA를 제 1항의 마이크로어래이 칩과 혼성화시키는 단계;3) hybridizing the cDNA labeled with different fluorescent substances of step 2) with the microarray chip of claim 1;

4) 반응한 마이크로어래이 칩을 분석하는 단계; 및4) analyzing the reacted microarray chip; And

5) 분석한 데이터에서 제 1항의 마이크로어래이 칩에 집적된 유전자 발현 정도를 대조군과 비교하여 확인하는 단계를 포함하는, 시료내 노닐페놀 노출 여부 검출 방법을 제공한다. 5) A method for detecting the presence or absence of nonylphenol in a sample, comprising the step of comparing the degree of gene expression integrated in the microarray chip of claim 1 with the control group.

상기 시료는 생체시료, 식품시료, 화학시료, 공업시료, 임상시료 및 환경시료로 구성된 군으로부터 선택되는 어느 하나인 것이 바람직하다. It is preferable that the sample is any one selected from the group consisting of a biological sample, a food sample, a chemical sample, an industrial sample, a clinical sample, and an environmental sample.

또한 상기 단계 2)의 형광물질은 Cy3, Cy5, FITC(poly L-lysine-fluorescein isothiocyanate), RITC(rhodamine-B-isothiocyanate) 및 로다민(rhodamine)으로 이루어진 군으로부터 선택되는 어느 하나인 것이 바람직하다. The fluorescent material of step 2) is preferably selected from the group consisting of Cy3, Cy5, poly L-lysine-fluorescein isothiocyanate (RITC), rhodamine-B-isothiocyanate (RITC), and rhodamine .

또한, 본 발명은 In addition,

1) 피검시료에 노출된 실험군의 바다송사리(Oryzias javanicus)와, 정상 대조군의 바다송사리에서 각각 RNA를 분리하는 단계;1) separating the RNA from the Oryzias javanicus of the test group exposed to the test sample and the sea lion of the normal control group, respectively;

2) 단계 1)의 RNA를, 서열번호 1 내지 123으로 기재되는 각각의 유전자에 상보적이고 유전자를 증폭할 수 있는 프라이머 쌍을 모두 사용하여 정량 실시간 RT-PCR(Quantitative real-time reverse transcript polymerase chain reaction, qRT-PCR)을 수행하는 단계; 및2) The RNA of step 1) was subjected to quantitative real-time reverse transcriptase polymerase chain reaction (RT-PCR) using both primer pairs complementary to each gene described in SEQ ID NOS: , qRT-PCR); And

3) 단계 2)의 유전자 산물을 대조군과 비교하여 발현 정도를 확인하는 단계를 포함하는, 시료내 노닐페놀 노출 여부 검출 방법을 제공한다. 3) comparing the gene product of step 2) with a control group to confirm the degree of expression, and detecting the presence or absence of nonylphenol in the sample.

또한, 본 발명은 상기 마이크로어래이 칩을 포함하는 노닐페놀 노출 여부 검출용 키트를 제공한다. The present invention also provides a kit for detecting whether or not to expose nonylphenol containing the microarray chip.

상기 키트는 스트렙타비딘-알칼리 탈인화효소 접합물질(strepavidin -like phosphatease conjugate), 화학형광물질(chemiflurorensce) 및 화학발광물질(chemiluminescent)로 이루어진 형광물질군으로부터 선택되는 어느 하나를 추가적으로 포함하는 것이 바람직하다.It is preferable that the kit further comprises any one selected from the group consisting of a fluorescent substance group consisting of a streptavidin-like phosphatease conjugate, a chemiluminescent substance and a chemiluminescent substance Do.

또한, 상기 키트는 혼성화에 사용되는 완충용액, RNA로부터 cDNA(complementary DNA)를 합성하기 위한 역전사효소, dNTPs(deoxynucleotide triphosphates) 및 rNTPs(ribonucleotide triPhosphates, 사전 혼합형 또는 분리 공급형), 표식시약, 및 세척 완충용액으로 이루어진 반응 시약군으로부터 선택되는 어느 하나를 추가적으로 포함하는 것이 바람직하다. In addition, the kit can be used as a buffer solution used for hybridization, a reverse transcriptase for synthesizing cDNA (complementary DNA) from RNA, deoxynucleotide triphosphates (dNTPs) and rNTPs (ribonucleotide triphosphates, pre-mixed or separated feed type) A buffer solution, and a buffer reagent.

아울러, 본 발명은 서열번호 1 내지 123으로 기재되는 각각의 유전자에 상보적이고 유전자를 증폭할 수 있는 프라이머 쌍을 모두 포함하는 노닐페놀 노출 여부 검출용 키트를 제공한다. In addition, the present invention provides a kit for detecting exposure to nonylphenol comprising all of the primer pairs complementary to each gene described in SEQ ID NOS: 1-123 and capable of amplifying a gene.

본 발명의 구체적인 실시예에서, 본 발명자들은 바다송사리를 배양하여 노닐페놀 20 ug/L 농도로 12, 24, 48 또는 72동안 노출 시킨 후, 이로부터 RNA를 분리하고, cDNA를 합성한 뒤, Cy3 및 Cy5로 표지하여 혼성화하였고, 바다송사리(Oryzias javanicus) 유전자 앤비헤즈 피쉬 배열(EnviHaz-Fish Array)를 활용한 이종간의 마이크로어래이 실험분석을 통해, 노닐페놀 노출에 의해 123종의 유전자 발현량이 대조군 대비 2배이상 증가 또는 감소하는 유전자를 선별하였다(표1 참조). 따라서, 상기 유전자들을 포함하는 바이오마커를 프로브로 이용하여 피검체와 대조군의 RNA를 추출하여 상기 마커 유전자들의 발현 변화를 비교분석함으로써, 피검체가 노닐페놀에 노출되었는지 여부를 검출하는데 유용하게 이용할 수 있다. In a specific example of the present invention, the present inventors cultured sea trouts and exposed 12, 24, 48, or 72% nonylphenol at a concentration of 20 ug / L, RNA was isolated therefrom, cDNA was synthesized, And Cy5. Hybridization was carried out by using microbial microarray analysis using an Oryzias javanicus gene EnviHaz-Fish Array. The expression levels of 123 genes were compared with those of the control group by nonylphenol exposure 2-fold increase or decrease was selected (see Table 1). Therefore, the biomarkers containing the genes are used as probes to extract the RNA of the test sample and the control group, and the change in the expression of the marker genes is comparatively analyzed, thereby being useful for detecting whether or not the subject is exposed to nonylphenol have.

이하 본 발명을 실시예에 의해서 상세히 설명한다. Hereinafter, the present invention will be described in detail with reference to Examples.

단 하기 실시예는 본 발명을 예시하기 위한 것일 뿐, 본 발명이 하기 실시예에 의해서 한정되는 것은 아니다. The following examples are for illustrative purposes only and are not intended to limit the scope of the present invention.

<< 실시예1Example 1 > > 바다송사리의Sea trout 배양 및 노닐페놀 노출  Cultivation and nonylphenol exposure

<1-1> <1-1> 바다송사리의Sea trout 배양 culture

바다송사리(Oryzias javanicus)는 세 종류의 필터(10, 10 및 1 ㎛)를 거친 자연해수에 배양하였다. 수중 히터를 이용하여 섭씨 25℃로 고정하였으며, 하루에 한번 아르테미아(Artemia) 유생을 먹이로 공급하였다. Oryzias javanicus was cultured in natural seawater through three different filters (10, 10 and 1 ㎛). It was fixed at 25 ° C using an underwater heater and once a day Artemia larvae were fed as feed.

<1-2> 노닐페놀(<1-2> Nonylphenol ( NonylphenolNonylphenol , NP) 노출, NP) Exposure

배양수조에서 사육중인 바다송사리 수컷을 여과된 해수 2 L가 담긴 3 L 비이커로 10 마리씩 옮기고, 24시간 동안 순치시켰다. Male offspring in breeding rabbits were transferred to a 3 L beaker containing 2 liters of filtered seawater and incubated for 24 hours.

상기 실시예 <1-1>의 바다송사리 수컷을 5 개체씩을 노닐페놀 20 ㎍/L에 12, 24, 48, 72시간 동안 노출하였다. 노출 농도는 송사리(O. latipes)의 노닐페놀 반수 치사농도(Lethal concentration 50, LC50, 0.85 mg/L 72 h)의 약 1/40에 해당되는 매우 낮은 수준의 농도로 결정하였다. 바다송사리를 한 마리씩 얼음물로 옮겨, 순간적으로 기절시킨 후, 머리를 잘라내고, 배를 갈라, 간을 적출하였다. Five male offspring male fishes of Example <1-1> were exposed to 20 μg / L of nonylphenol for 12, 24, 48 and 72 hours, respectively. The exposure concentration was determined at a very low level of concentration corresponding to about 1/40 of the nonylphenol half-life (Lethal concentration 50, LC 50, 0.85 mg / L 72 h) of O. latipes . They moved one sea lion to ice water, instantly stunned them, then cut their heads, split their belly, and extracted the liver.

<< 실시예Example 2> 노닐페놀 노출에 의한 유전자 발현량 변화 측정 2> Measurement of gene expression level by exposure to nonylphenol

<2-1> RNA의 분리<2-1> Isolation of RNA

상기 실시예 <1-2>에서 수득한 노닐페놀 노출 시험군(12, 24, 48, 72 시간) 및 노출하지 않은 대조군의 바다송사리 간에 TRI Reagent 용액(Molecular Research Center Inc, Cincinnati, Ohaio, USA) 1 ㎖를 넣고, 유리 균질기(glass homogenizer)를 이용하여 균질화하고, 실온에 5 분간 방치하였다. 클로로포름 200 ㎕를 첨가하여 잘 섞고, 실온에서 10 분간 방치하고, 15 분간 원심분리(12,000g, 4℃)하였다. 상층액을 취하여, 아이소프로판올(isopropanol) 500 ㎕를 넣고, 상온에 5 분간 방치하였다. 약 20 분간 원심분리(12,000×g, 4℃)한 후, 용액을 제거하여 침전물만을 취했다. 상기 침전물에 70% 에탄올 용액 50 ㎕를 넣어 5 분간 원심분리한 뒤, 에탄올 용액을 제거하고 침전된 RNA를 건조시켰다. 건조 후, 적당량의 에틸피로카보네이트(diethylpyrocarbonate, DEPC)에 용해하였다.A TRI Reagent solution (Molecular Research Center Inc, Cincinnati, Ohao, USA) was applied between the nonylphenol exposure test group (12, 24, 48, 72 hours) obtained in Example <1-2> 1 ml, homogenized using a glass homogenizer, and allowed to stand at room temperature for 5 minutes. 200 μl of chloroform was added, mixed well, left at room temperature for 10 minutes, and centrifuged (12,000 g, 4 ° C) for 15 minutes. The supernatant was taken, 500 μl of isopropanol was added, and the mixture was allowed to stand at room temperature for 5 minutes. After centrifugation (12,000 x g, 4 캜) for about 20 minutes, the solution was removed and only the precipitate was taken. 50 μl of a 70% ethanol solution was added to the precipitate, followed by centrifugation for 5 minutes. Then, the ethanol solution was removed and the precipitated RNA was dried. After drying, it was dissolved in an appropriate amount of ethyl pyrocarbonate (DEPC).

<2-2> <2-2> Cy3Cy3 및  And Cy5Cy5 표지  sign

정제된 노출군 및 대조군의 전체 RNA는 Agilent's Low RNA Input Linear Amplification Kit Plus (Agilent Technologies, USA)를 사용하여 다음과 같이 Cy3 및 Cy5로 표지하였다.The total RNAs from the purified exposed and control groups were labeled with Cy3 and Cy5 using Agilent's Low RNA Input Linear Amplification Kit Plus (Agilent Technologies, USA) as follows.

구체적으로, RNA 1 ㎍을 dT-프로모터 프라이머(dT-promoter primer)와 MMLV-역전사효소(MMLV-Reverse transcriptase)와 혼합하여 40℃에서 2시간 동안 역전사반응을 수행하였다. 그런 다음, T7 중합효소(T7 polymerase)를 첨가하여 40℃에서 2시간 동안 선형 증폭(linear amplification)을 수행하였다. 이와 같은 증폭 과정을 통해 실험군 및 대조군의 시료를 각각 Cy3-CTP와 Cy5-CTP로 표지하였다.Specifically, 1 μg of RNA was mixed with dT-promoter primer (dT-promoter primer) and MMLV-reverse transcriptase and reverse transcription was performed at 40 ° C. for 2 hours. Then, T7 polymerase (T7 polymerase) was added and linear amplification was performed at 40 DEG C for 2 hours. Through the amplification process, the samples of the experimental group and the control group were labeled with Cy3-CTP and Cy5-CTP, respectively.

<2-3> <2-3> 혼성화Hybridization (Hybridization) 및 스캐닝(scanning) (Hybridization) and scanning (scanning)

노닐페놀 노출에 따라 발현량이 변화되는 유전자들을 얻기위한 차등발현 유전자 프로파일링에는 EnviHaz-Fish Array가 사용되었다. 이 마이크로어래이에는 바다송사리 간기원의 유전자 22,000여개에 해당되는 oligo-probe가 탑재되어 있다.The EnviHaz-Fish Array was used for profiling differentially expressed genes to obtain genes whose expression levels were changed by nonylphenol exposure. The microarray contains over 22,000 oligo-probes, which are originated from the sea lily of the sea.

형광물질이 라벨링된 cRNA 시료를 Qiagen PCR purification kit을 사용하여 정제하고, 증류수로 용출하였다. 정제된 형광표지-cRNA 시료를 혼성화 완충액(hybridization buffer)(3x SSC, 0.3% SDS, 50% 포름아미드(formamide), 20 ㎍ Cot-1 DNA, 20 ㎍ 효모균(yeast) tRNA)에 첨가한 후, microcon YM-30으로 농축하여 혼성화 혼합물을 만들었다. 혼성화 혼합물 95℃로 3분 동안 가열하여 변성시키고 12,000 g에서 30초간 원심분리하며 온도를 식혔다. 제조된 바다송사리 마이크로어래이 (EnviHaz-Fish Array)에 커버슬립(coverslip)을 덮고, 변성시킨 혼성화 혼합물을 파이펫팅(pipetting)하였다. 마이크로어래이를 GT-Hyb 챔버(Chamber)에 넣고 65℃에서 16시간 동안 반응시켰다. 혼성화가 끝난 후, 챔버에서 마이크로어래이를 꺼내어 아래의 순서대로 세척과정을 수행하고, 마이크로어래이를 회전하여 건조한 후 스캐닝 할 때까지 암실에서 보관하였다. 실험이 완료된 바다송사리 마이크로어레이를 Axon GenePix 4000B scanner(Axon Instrument, USA)를 사용하여 스캔하였다. GenePix Pro 6.0 program에서, 스캔 이미지로부터 각 점을 그리 딩 파일(gridding file)을 이용하여 그리딩하고, 정량화하여 각 점의 Cy5/Cy3 강도 및 비율등의 분석값이 포함된 GPR 파일(GPR file)을 얻었다.Fluorescent labeled cRNA samples were purified using Qiagen PCR purification kit and eluted with distilled water. The purified fluorescent marker-cRNA sample was added to a hybridization buffer (3 x SSC, 0.3% SDS, 50% formamide, 20 Cot Cot-1 DNA, 20 ㎍ yeast tRNA) and concentrated with microcon YM-30 to form a hybridization mixture. The hybridization mixture was denatured by heating at 95 DEG C for 3 minutes, centrifuged at 12,000 g for 30 seconds, and the temperature was cooled. The thus-prepared EnviHaz-Fish Array was covered with a coverslip, and the modified hybridization mixture was pipetted. The microarray was placed in a GT-Hyb chamber and reacted at 65 ° C for 16 hours. After hybridization was completed, the micro flounder was removed from the chamber and washed in the following order. The micro flounder was rotated, dried and stored in a dark room until scanning. After the experiment, the sea microsleigh array was scanned using an Axon GenePix 4000B scanner (Axon Instrument, USA). In GenePix Pro 6.0 program, GPR file (GPR file) containing analysis values such as Cy5 / Cy3 intensity and ratio of each point by drawing and quantifying each point from a scanned image using a gridding file, &Lt; / RTI &gt;

<< 실시예Example 3>  3> 마이크로어래이Microarray 자료 분석  Data analysis

GenePix Pro Program에서 얻어진 GPR 파일로부터, 분석 프로그램인 GeneSpring 7.3.1(Agilent Technologies, USA)를 이용하여 분석을 수행하였다. 표준화(Normalization): LOWESS(locally weighted regression scatterplot smoothing)를 이용하여 수행하였다. 신뢰할 수 있는 유전자(Reliable gene): 중앙값의 합이 배경(background) 보다 낮거나, 각 화소(pixel) 값의 표준편차가 유의하지 않은 점을 플래그 아웃(flag-out)함으로써 유의한 유전자를 얻었다. 유의한 유전자(Significant genes) : 평준화된 비율(normalized ratio) 값이 2 배 이상 차이를 보이는 점을 선별하였다. From the GPR files obtained from the GenePix Pro program, analysis was performed using the analysis program GeneSpring 7.3.1 (Agilent Technologies, USA). Normalization: LOWESS (locally weighted regression scatterplot smoothing) was used. Reliable gene: Significant genes were obtained by flagging out that the sum of the median values is lower than the background, or the standard deviation of each pixel value is not significant. Significant genes: We selected the point where the normalized ratio difference is more than 2 times.

<3-1> 특이유전자후보군의 확인<3-1> Identification of candidate genes for specific genes

1차적으로 트리클로산 노출 시간별 유전자 발현량이 2배이상 변화되는 유전자들을 선별하였다. 노출 실험은 3회 실시하였으며, 통계적으로 유의한 유전자들을 선별하였다. Genes were selected whose gene expression levels were changed by more than 2 times in terms of exposure time of triclosan. Exposure experiments were performed 3 times and statistically significant genes were selected.

그 결과, 12, 24, 48, 72시간 동안의 노닐페놀 노출에 의해 유전자 발현량이 대조군에 비해 2배 이상 증가 또는 감소하는 유전자들 123종을 새롭게 확인하여, 이들을 서열번호 1 내지 123으로 기재하였다(표 1). As a result, 123 kinds of genes in which the amount of gene expression was increased or decreased by 2 times or more than that of the control group by the exposure to nonylphenol for 12, 24, 48, and 72 hours were newly identified and described as SEQ ID NOs: 1 to 123 Table 1).

노닐페놀 노출에 의해 발현량이 2배이상 변화되는 유전자 및 그 변화량 The gene whose expression level is changed more than 2 times by nonylphenol exposure and its change amount 서열번호SEQ ID NO: 유전자gene 발현량 변화Change in expression level 1st 1st 2nd 2nd 3rd 3rd 1One insulin-like insulin-like -39.79 -39.79 -85.96 -85.96 -97.29 -97.29 22 complement C1q-like protein 2-like (A)complement C1q-like protein 2-like (A) -13.81 -13.81 -8.60 -8.60 -7.76 -7.76 33 trypsin-3-liketrypsin-3-like -3.85 -3.85 -17.04 -17.04 -25.45 -25.45 44 transmembrane protease serine 9-like transmembrane protease serine 9-like -3.49 -3.49 -12.04 -12.04 -18.55 -18.55 55 glucagon-1-like glucagon-1-like -5.97 -5.97 -7.16 -7.16 -8.48 -8.48 66 alpha-amylase 1-like isoform 1 alpha-amylase 1-like isoform 1 -3.50 -3.50 -10.35 -10.35 -17.78 -17.78 77 pancreatic alpha-amylasepancreatic alpha-amylase -3.35 -3.35 -11.30 -11.30 -17.56 -17.56 88 three finger toxin MALT0070C-likethree finger toxin MALT0070C-like -2.40 -2.40 -20.26 -20.26 -76.67 -76.67 99 chymotrypsin-like elastase family member 2A-like (A) chymotrypsin-like elastase family member 2A-like (A) -2.64 -2.64 -15.04 -15.04 -29.05 -29.05 1010 guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-8-likeguanine nucleotide-binding protein G (I) / G (S) / G (O) subunit gamma-8-like -10.83 -10.83 -6.36 -6.36 -4.15 -4.15 1111 complement C1q-like protein 2-like (B)complement C1q-like protein 2-like (B) -5.93 -5.93 -6.71 -6.71 -5.24 -5.24 1212 lymphocyte antigen 6D-likelymphocyte antigen 6D-like -6.51 -6.51 -6.19 -6.19 -4.84 -4.84 1313 chymotrypsin-like elastase family member 2A-like (B)chymotrypsin-like elastase family member 2A-like (B) -2.49 -2.49 -9.94 -9.94 -29.82 -29.82 1414 elastase-1-likeelastase-1-like -2.94 -2.94 -8.46 -8.46 -12.44 -12.44 1515 Vacuolar protein sorting-associated protein 52 homolog Vacuolar protein sorting-associated protein 52 homolog -37.94 -37.94 -4.77 -4.77 -3.27 -3.27 1616 trypsin-liketrypsin-like -3.12 -3.12 -7.70 -7.70 -10.28 -10.28 1717 Ubiquitin-like proteinUbiquitin-like protein -2.95 -2.95 -6.29 -6.29 -12.85 -12.85 1818 matrix-remodeling-associated protein 8-like (A)matrix-remodeling-associated protein 8-like (A) -2.25 -2.25 -21.35 -21.35 -11.44 -11.44 1919 astacin like metallo-protease precursorastacin-like metallo-protease precursor -2.80 -2.80 -5.21 -5.21 -27.84 -27.84 2020 keratin, type I cytoskeletal 13-likekeratin, type I cytoskeletal 13-like -2.76 -2.76 -10.43 -10.43 -5.20 -5.20 2121 chymotrypsin B-likechymotrypsin B-like -2.56 -2.56 -5.28 -5.28 -8.40 -8.40 2222 L-SF precursorL-SF precursor -25.55 -25.55 -2.45 -2.45 -3.88 -3.88 2323 kinesin-like protein KIF1A-likekinesin-like protein KIF1A-like -4.02 -4.02 -4.11 -4.11 -3.89 -3.89 2424 heme oxygenase-likeheme oxygenase-like -7.49 -7.49 -2.86 -2.86 -3.72 -3.72 2525 carboxypeptidase A2-likecarboxypeptidase A2-like -2.04 -2.04 -6.43 -6.43 -8.35 -8.35 2626 type-4 ice-structuring protein LS-12-like isoform 1type-4 ice-structuring protein LS-12-like isoform 1 -2.20 -2.20 -6.27 -6.27 -6.49 -6.49 2727 carboxypeptidase B-likecarboxypeptidase B-like -2.54 -2.54 -3.80 -3.80 -8.13 -8.13 2828 acyl-coenzyme A thioesterase 5-likeacyl-coenzyme A thioesterase 5-like -21.06 -21.06 -3.12 -3.12 -2.31 -2.31 2929 placenta-specific gene 8 protein-likeplacenta-specific gene 8 protein-like -11.78 -11.78 -2.67 -2.67 -2.86 -2.86 3030 protein S100-P-likeprotein S100-P-like -2.16 -2.16 -3.22 -3.22 -19.53 -19.53 3131 neurogenic locus notch homolog protein 1-likeneurogenic locus notch homolog protein 1-like -2.54 -2.54 -2.49 -2.49 -32.97 -32.97 3232 insulin-induced gene 1 protein-likeinsulin-induced gene 1 protein-like -4.57 -4.57 -2.71 -2.71 -4.14 -4.14 3333 alpha-amylase 1-like isoform 2alpha-amylase 1-like isoform 2 -2.13 -2.13 -3.35 -3.35 -12.50 -12.50 3434 somatostatin-2-likesomatostatin-2-like -3.88 -3.88 -2.34 -2.34 -5.33 -5.33 3535 C-reactive protein-likeC-reactive protein-like -6.08 -6.08 -4.78 -4.78 -2.00 -2.00 3636 chymotrypsin-like elastase family member 2B-likechymotrypsin-like elastase family member 2B-like -2.05 -2.05 -3.30 -3.30 -11.16 -11.16 3737 microfibril-associated glycoprotein 4-like (A)microfibril-associated glycoprotein 4-like (A) -3.90 -3.90 -2.09 -2.09 -6.79 -6.79 3838 microfibril-associated glycoprotein 4-like (B)microfibril-associated glycoprotein 4-like (B) -3.84 -3.84 -2.08 -2.08 -6.83 -6.83 3939 claudin-15-likeclaudin-15-like -2.37 -2.37 -2.53 -2.53 -13.10 -13.10 4040 h-2 class II histocompatibility antigen gamma chain-likeh-2 class II histocompatibility antigen gamma chain-like -8.95 -8.95 -2.15 -2.15 -3.15 -3.15 4141 cone type phosducincone type phosducin -2.91 -2.91 -10.67 -10.67 -2.11 -2.11 4242 cysteine-rich venom protein kaouthin-1-like cysteine-rich venom protein kaouthin-1-like -2.04 -2.04 -3.04 -3.04 -9.65 -9.65 4343 microfibril-associated glycoprotein 4-like isoform 1microfibril-associated glycoprotein 4-like isoform 1 -4.23 -4.23 -2.30 -2.30 -3.98 -3.98 4444 microfibril-associated glycoprotein 4-like (C)microfibril-associated glycoprotein 4-like (C) -3.39 -3.39 -2.09 -2.09 -6.03 -6.03 4545 ras association domain-containing protein 6-likeras association domain-containing protein 6-like -2.44 -2.44 -3.13 -3.13 -4.70 -4.70 4646 apolipoprotein Eb-likeapolipoprotein Eb-like -17.24 -17.24 -2.45 -2.45 -2.03 -2.03 4747 class I histocompatibility antigen, F10 alpha chain-like isoform X3class I histocompatibility antigen, F10 alpha chain-like isoform X3 -2.62 -2.62 -5.85 -5.85 -2.30 -2.30 4848 SH2 domain-containing protein 4A-likeSH2 domain-containing protein 4A-like -2.74 -2.74 -3.46 -3.46 -2.99 -2.99 4949 c-fos proteinc-fos protein -2.12 -2.12 -2.57 -2.57 -7.56 -7.56 5050 beta-microseminoprotein-likebeta-microseminoprotein-like -2.63 -2.63 -4.34 -4.34 -2.47 -2.47 5151 F-box only protein 2-like F-box only protein 2-like -2.03 -2.03 -3.97 -3.97 -3.45 -3.45 5252 matrix-remodeling-associated protein 8-like (B)matrix-remodeling-associated protein 8-like (B) -2.74 -2.74 -2.28 -2.28 -4.05 -4.05 5353 CD59B glycoprotein-like isoform X1CD59B glycoprotein-like isoform X1 -4.90 -4.90 -2.42 -2.42 -2.16 -2.16 5454 glyceraldehyde 3-phosphate dehydrogenase 2-likeglyceraldehyde 3-phosphate dehydrogenase 2-like -4.51 -4.51 -2.13 -2.13 -2.57 -2.57 5555 complement C1q subcomponent subunit A-like, partialcomplement C1q subcomponent subunit-like, partial -3.61 -3.61 -2.03 -2.03 -3.08 -3.08 5656 DNA repair protein XRCC1-likeDNA repair protein XRCC1-like -2.98 -2.98 -2.18 -2.18 -3.27 -3.27 5757 DAP10 precursorDAP10 precursor -3.28 -3.28 -2.37 -2.37 -2.33 -2.33 5858 betaine--homocysteine S-methyltransferase 1-likebetaine-homocysteine S-methyltransferase 1-like -2.71 -2.71 -2.99 -2.99 -2.06 -2.06 5959 coronin-1A-likecoronin-1A-like -2.42 -2.42 -2.36 -2.36 -2.65 -2.65 6060 rho-related GTP-binding protein RhoG-like isoform 1rho-related GTP-binding protein RhoG-like isoform 1 -2.51 -2.51 -2.30 -2.30 -2.44 -2.44 6161 actin-related protein 2/3 complex subunit 1B-likeactin-related protein 2/3 complex subunit 1B-like -2.35 -2.35 -2.23 -2.23 -2.13 -2.13 6262 high affinity immunoglobulin epsilon receptor subunit gamma-likehigh affinity immunoglobulin epsilon receptor subunit gamma-like -2.03 -2.03 -2.29 -2.29 -2.40 -2.40 6363 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial-like1,25-dihydroxyvitamin D (3) 24-hydroxylase, mitochondrial-like -15.24 -15.24 2.28 2.28 -5.03 -5.03 6464 immunoglobulin lambda-like polypeptide 1-likeimmunoglobulin lambda-like polypeptide 1-like 2.18 2.18 -3.07 -3.07 -10.24 -10.24 6565 gamma-crystallin N-A-likegamma-crystallin N-A-like -3.72 -3.72 -4.35 -4.35 2.13 2.13 6666 aminopeptidase O-likeaminopeptidase O-like 2.19 2.19 -2.54 -2.54 -2.78 -2.78 6767 ES1 protein homolog, mitochondrial-like isoform 2ES1 protein homolog, mitochondrial-like isoform 2 2.75 2.75 -3.69 -3.69 -16.08 -16.08 6868 RNA-directed DNA polymerase from mobile element jockey-like (A)RNA-directed DNA polymerase from mobile element jockey-like (A) -2.94 -2.94 2.26 2.26 -2.01 -2.01 6969 solute carrier family 22 member 5-likesolute carrier family 22 member 5-like -2.60 -2.60 -11.73 -11.73 2.71 2.71 7070 complement C1q-like protein 4-likecomplement C1q-like protein 4-like -2.37 -2.37 2.47 2.47 -2.65 -2.65 7171 chymotrypsinogen 2-like isoform 1chymotrypsinogen 2-like isoform 1 2.82 2.82 -4.18 -4.18 -4.16 -4.16 7272 catenin alpha-2 isoform X4catenin alpha-2 isoform X4 2.77 2.77 -2.20 -2.20 -6.87 -6.87 7373 N-acylglucosamine 2-epimerase-likeN-acylglucosamine 2-epimerase-like -6.12 -6.12 -3.77 -3.77 2.94 2.94 7474 plakophilin-3-likeplakophilin-3-like -4.48 -4.48 -2.58 -2.58 3.09 3.09 7575 keratin, type I cytoskeletal 18-likekeratin, type I cytoskeletal 18-like -2.03 -2.03 3.21 3.21 -8.29 -8.29 7676 thiosulfate sulfurtransferase/rhodanese-like domain-containing protein 1-likethiosulfate sulfurtransferase / rhodanese-like domain-containing protein 1-like -2.32 -2.32 3.31 3.31 -3.33 -3.33 7777 protein disulfide-isomerase A2-likeprotein disulfide-isomerase A2-like 4.00 4.00 -3.82 -3.82 -3.71 -3.71 7878 inward rectifier potassium channel 16-likeinward rectifier potassium channel 16-like -2.81 -2.81 -2.07 -2.07 3.76 3.76 7979 dehydrogenase/reductase SDR family member on chromosome X-likedehydrogenase / reductase SDR family member on chromosome X-like 2.28 2.28 -2.46 -2.46 2.10 2.10 8080 2-epi-5-epi-valiolone synthase-like2-epi-5-epi-valiolone synthase-like 4.62 4.62 -8.12 -8.12 -18.23 -18.23 8181 major histocompatibility complex class I-related gene protein-like precursormajor histocompatibility complex class I-related gene protein-like precursor 4.62 4.62 -5.88 -5.88 -12.91 -12.91 8282 aquaporin-12-likeaquaporin-12-like -2.28 -2.28 2.37 2.37 2.10 2.10 8383 RNA-directed DNA polymerase from mobile element jockey-like (B)RNA-directed DNA polymerase from mobile element jockey-like (B) -10.89 -10.89 -2.02 -2.02 4.48 4.48 8484 78 kDa glucose-regulated protein-like precursor78 kDa glucose-regulated protein-like precursor -2.56 -2.56 2.74 2.74 2.03 2.03 8585 hemoglobin subunit beta-likehemoglobin subunit beta-like 2.27 2.27 -2.13 -2.13 3.16 3.16 8686 splicing factor U2AF 35 kDa subunit-like isoform X2splicing factor U2AF 35 kDa subunit-like isoform X2 2.05 2.05 2.04 2.04 2.17 2.17 8787 long-chain-fatty-acid--CoA ligase ACSBG2-likelong-chain-fatty-acid-CoA ligase ACSBG2-like 3.42 3.42 2.40 2.40 -2.03 -2.03 8888 vitelline membrane outer layer protein 1 homologvitelline membrane outer layer protein 1 homolog 5.89 5.89 -5.65 -5.65 -3.76 -3.76 8989 diphosphomevalonate decarboxylase-likediphosphomevalonate decarboxylase-like 2.30 2.30 3.74 3.74 -3.01 -3.01 9090 solute carrier family 15 member 1-likesolute carrier family 15 member 1-like -2.34 -2.34 -2.13 -2.13 5.49 5.49 9191 extracellular calcium-sensing receptor-likeextracellular calcium-sensing receptor-like 3.14 3.14 -2.46 -2.46 2.87 2.87 9292 macrophage-capping protein-like메크피 파그 cap cap 3.79 3.79 -2.34 -2.34 2.23 2.23 9393 transducin beta-like protein 2-like isoform 2transducin beta-like protein 2-like isoform 2 -2.38 -2.38 4.12 4.12 2.31 2.31 9494 squalene synthase-likesqualene synthase-like 3.29 3.29 3.42 3.42 -6.95 -6.95 9595 carboxypeptidase A1-likecarboxypeptidase A1-like 6.55 6.55 -5.81 -5.81 -4.54 -4.54 9696 CTP synthase 1-likeCTP synthase 1-like 2.93 2.93 2.20 2.20 2.04 2.04 9797 ethanolamine-phosphate cytidylyltransferase-likeethanolamine-phosphate cytidylyltransferase-like 3.22 3.22 4.13 4.13 -4.71 -4.71 9898 guanine nucleotide-binding protein-like 3-likeguanine nucleotide-binding protein-like 3-like 3.29 3.29 2.16 2.16 2.12 2.12 9999 rab3 GTPase-activating protein non-catalytic subunit-likerab3 GTPase-activating protein non-catalytic subunit-like 2.20 2.20 2.43 2.43 3.01 3.01 100100 L-xylulose reductase-likeL-xylulose reductase-like 3.30 3.30 2.00 2.00 2.49 2.49 101101 pentraxin fusion protein-likepentraxin fusion protein-like -5.57 -5.57 4.04 4.04 3.58 3.58 102102 mitochondrial import inner membrane translocase subunit Tim21-likemitochondrial import inner membrane translocase subunit Tim21-like 3.61 3.61 2.05 2.05 2.32 2.32 103103 bile salt-activated lipase-likeeven salt-activated lipase-like 7.70 7.70 -7.80 -7.80 -4.95 -4.95 104104 peptidyl-prolyl cis-trans isomerase FKBP11-like peptidyl-prolyl cis-trans isomerase FKBP11-like -2.59 -2.59 4.16 4.16 3.60 3.60 105105 sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating-likesterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating-like 4.43 4.43 3.90 3.90 -3.40 -3.40 106106 cleavage and polyadenylation specificity factor subunit 2-likecleavage and polyadenylation specificity factor subunit 2-like 3.18 3.18 2.86 2.86 2.64 2.64 107107 ribosome biogenesis protein bop1-likeribosome biogenesis protein bop1-like 3.83 3.83 2.64 2.64 2.27 2.27 108108 somatostatin-1-likesomatostatin-1-like 8.69 8.69 -4.86 -4.86 -6.40 -6.40 109109 lipocalin-likelipocalin-like 3.90 3.90 5.24 5.24 -37.36 -37.36 110110 39S ribosomal protein L12, mitochondrial-like39S ribosomal protein L12, mitochondrial-like 3.94 3.94 2.56 2.56 2.85 2.85 111111 interferon-induced protein with tetratricopeptide repeats 1-like interferon-induced protein with tetratricopeptide repeats 1-like -2.55 -2.55 3.22 3.22 6.01 6.01 112112 UDP-glucuronosyltransferase 1-5-likeUDP-glucuronosyltransferase 1-5-like 4.08 4.08 2.77 2.77 3.16 3.16 113113 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase-like3-beta-hydroxysteroid-Delta (8), Delta (7) -isomerase-like 6.66 6.66 3.50 3.50 -5.79 -5.79 114114 elongation of very long chain fatty acids protein 5-likeelongation of very long chain fatty acids protein 5-like 7.45 7.45 3.50 3.50 -2.03 -2.03 115115 collagen alpha-1(VI) chain-like collagen alpha-1 (VI) chain-like -3.15 -3.15 3.64 3.64 7.86 7.86 116116 insulinoma-associated protein 1-like insulinoma-associated protein 1-like 12.56 12.56 -4.15 -4.15 -3.64 -3.64 117117 PTB domain-containing engulfment adapter protein 1-like The PTB domain-containing engulfment adapter protein 1-like 5.43 5.43 5.36 5.36 2.92 2.92 118118 protein S100-A13-likeprotein S100-A13-like 5.76 5.76 8.54 8.54 -13.72 -13.72 119119 fatty acid-binding protein, brain-likefatty acid-binding protein, brain-like -11.18 -11.18 6.37 6.37 9.38 9.38 120120 nidogen-2-likenidogen-2-like 5.34 5.34 10.00 10.00 3.09 3.09 121121 glutamate decarboxylase-like protein 1-likeglutamate decarboxylase-like protein 1-like 6.68 6.68 3.74 3.74 9.55 9.55 122122 DNA damage-inducible transcript 4 protein-like DNA damage-inducible transcript 4 protein-like 5.93 5.93 21.44 21.44 -18.80 -18.80 123123 fatty acid desaturase 2-like fatty acid desaturase 2-like 79.79 79.79 6.61 6.61 -8.19-8.19

발현량 변화에서, 양수는 발현량의 증가, 음수는 발현량의 감소 배율을 나타낸다.In the change of the expression amount, the positive number indicates an increase in the expression amount, and the negative number indicates the decrease amount of the expression amount.

<110> Korea Institute of Ocean Science & Technology <120> Nonylphenol responsive genes in Oryzias javanicus and the method for diagnosing aquatic environment pollution using the same <160> 123 <170> KoPatentIn 3.0 <210> 1 <211> 232 <212> DNA <213> Oryzias sp. <400> 1 cgggaccaga tgaatgccat ggcaaaacga aacattctgg agcgctgctg ttacatgccc 60 tgcaccatct acgacctggc gaactattgt tcctgaagag ccggcgcaga caccaatcgc 120 tgttagatta gcttacagag cagtattcaa ttagctgttc aagtgtcagc aacagtagtt 180 cttattgcta gctatccgtt tttaaagaat gtgctgataa attattcaaa at 232 <210> 2 <211> 325 <212> DNA <213> Oryzias sp. <400> 2 tttccaatgt tggtcacgac cttcttgaat tcaagggttt tgtctgcctc aaacggcccc 60 aaagtggtcc attcctgggt ctcaagaagt gcagcagaga aggccacctg tctcgctgat 120 acatccctct ggagctcctc cactttggtt gttagctgtt gcactttggt gttcagctcc 180 tgcagtgtgg ggtcctgagc ccccaccaaa ccacaggcaa gagccaccag aaggatccag 240 ctggacatca tctttgctaa gagagaaccg ggcttaaagc aggctccccg gccgtaatgg 300 ccactctgcg ttgataccac tgctt 325 <210> 3 <211> 383 <212> DNA <213> Oryzias sp. <400> 3 aagcagtggt atcaacgcag agtggccatt acggccgggg gtgtctcgac gcaggaagca 60 gccatgaagt tcttcattct cctggccctt tgtgctgcag catatgctgc tcctcttgag 120 gatgacaaga tcgttggggg ttacgagtgc aggaagaact ctgtgcccta ccaggtgtct 180 ctgaatgtgg gctaccactt ctgtggaggc tccctgatct ccagcacctg ggtggtgtcc 240 gctgctcact gctaccagtc tcgtatccag gtgcgtctcg gtgagcacaa cattgctgtg 300 aacgagggaa ccgagcagtt catcgactcc gccaagatca tccgtcaccc cagctacagc 360 agctggaacc tggacaacga cat 383 <210> 4 <211> 155 <212> DNA <213> Oryzias sp. <400> 4 aagcagtggt atcaacgcag agtggccatt acggccgggg tcatcaacag gatcactcag 60 cagccatgag gtctctggtc ttcgttctgc tcattggagc cgccttcgcc cttgatgacg 120 acaagatcgt cggagggtat gagtgcaccc cctac 155 <210> 5 <211> 685 <212> DNA <213> Oryzias sp. <400> 5 aagcagtggt atcaacgcag agtgccatta cggccgggga gagacaacaa gagaaggaac 60 taagtgccac ttgggaagaa gaaaaacaga aggttacaac catgaaaagc ctccagtcgc 120 tggccactat cctcctcctc ctcggctttt tccacaacag ctggcaggtt cctctactcc 180 agaacgagga cagctccagt tttacagcag acgacacgct agctgatgag ctgcgggagc 240 tgtccagcat gaagagacac tcggagggaa ccttctccaa cgactacagc aaatatctgg 300 aggacaggaa ggcgcaggac ttcgttcggt ggctgatgaa caacaaaagg agcggtgatg 360 caggcaagcg ccatgcagat ggtaccttca ccagcgacgt gagcgcctac ctcaaggagc 420 aggcaatcaa agactttgtt gccaagctga aggccggtca aatcccaaga gagtagggtt 480 gtccttcgta tctcctcctc ctcctcctcc tgcagtgaaa acgacccgtg tctgatcctg 540 gcgaccgacg agccaatcat ctgcttcttg ttcttcttct ggcgcttttt ccctgatttt 600 cccaaaataa aaatcaccta aacttttaaa aaaaaaaaaa aaaaaaaaag gccgcctcgg 660 ccactctgcg ttgataccac tgctt 685 <210> 6 <211> 470 <212> DNA <213> Oryzias sp. <400> 6 ggtgcaggag gagcctccat cctcaccttc tgggatccca gactttacaa gatggctgtg 60 ggatacatgc tggctcatcc ttacggattc accagagtca tgtctagcta ccgctgggac 120 cgcaactttg tgaacggaaa ggatcagaat gactggattg gcccccccag caacggagac 180 ggatccacca agccagttcc catcaaccct gaccagacgt gtggagatgg atgggtgtgt 240 gagcacagat ggcgtcagat tacgaacatg gtccaattcc gtaatgtggt cagtggacag 300 cctcatgcca actggtggga caacggcaac aatcaggtcg cctttggccg cggaaaccgt 360 ggattcatcg tgttcaacaa tgacgactgg gctctggatg tgacgctgaa cactggcctt 420 cctggcggca cctactgtga cgtcatctct ggcaataaga acggaggcag 470 <210> 7 <211> 110 <212> DNA <213> Oryzias sp. <400> 7 ggatacatgc tggctcatcc ttacggattc accagagtca tgtctagcta ccgctgggac 60 cgcaactttg tgaacggaaa ggatcagaat gactggattg gcccccccag 110 <210> 8 <211> 397 <212> DNA <213> Oryzias sp. <400> 8 aagcagtggt atcaacgcag agtgccatta cggccgggaa aaaaattctc agattttctg 60 gagagacgac agagttaacg gacatgaagc ctgtcattct tacggttctg gttctgctgc 120 tcgtcagtca gaatgaggct ttgaagtgtt actgtgaaaa cacggtggtt tgtgacggac 180 ccgtccacga gtgtaaacct gaagaaacgg tctgtctggc gacggccctt cttcaagtcg 240 gtcgatactt caggaggtgt gcagaaccca gcgcatgctc caacctgaat gtacctggtg 300 ttgcctattc cacttgctgc aggaccgact tgtgcaactg atgctctgga gccgattaaa 360 actgctttga attcctcaat aaaaatctat tttacac 397 <210> 9 <211> 130 <212> DNA <213> Oryzias sp. <400> 9 aagcagtggt atcaacgcag agtggccatt acggccgggg agtgacggtc acaatgaagt 60 tcctgatctt ggctttgttt gttgctggtg cctatggttg tggcctgccc acataccccc 120 ctgttgttac 130 <210> 10 <211> 635 <212> DNA <213> Oryzias sp. <400> 10 aagcagtggt atcaacgcag agtggccatt acggccgggg gagaccttcc ctgcaccacc 60 tgcagctgag acccagggag cagaattaac aagcaaattg aaacagactc caaccagcgt 120 tggagatgct ttcaataata tgttgttcat taagtcgggc tctttgttgt ttttgttttt 180 ttgcaactga atctctgctc tctctcgctt gctgccagct gactgacgtt tagtgggaga 240 agaaaaggaa aggatgtcca acaacatggc caagattgca gatgctagaa agactgtgga 300 gcaactgaaa cttgaggtca acatagagag gatgatggtg tcaaaagctg cagccgagct 360 gatgtccttc tgtgaatctc acgccaaaga ggaccctctg gtgacaccgg taccttcatc 420 agaaaaccct ttacagagaa aagaaagctt ttctgtgcca ttctgtgacc ccctacgacc 480 ctccaaagta gacatgtagc acaaaaatta acatttttat ttgtttttag agtcaatgca 540 gctcttttgc attgatctat gagcatgttt agaaaaaaaa aanaaaaaaa aaaaaaaaag 600 gccgcctcgg ccactctgcg ttgataccac tgctt 635 <210> 11 <211> 247 <212> DNA <213> Oryzias sp. <400> 11 tggatataac ccagagacag gcatcttcac ggctccagtc aaagggctgt actacttcag 60 ggtcactggg attgctggga gcaatgggga attaaacgca ggactcaaaa agaacggaca 120 gaacatgttt gcaatctacc agaaggcagg aacacaggct ggcacctcaa acgggatggc 180 tctggaactg gagcagggtg accgggtcta cgtccagatt tggccaaact tgaccgtggc 240 tgaccag 247 <210> 12 <211> 697 <212> DNA <213> Oryzias sp. <400> 12 aagcagtggt atcaacgcag agtggcatta cggccgggga tacacctgcc aacatgaagg 60 ttatgctgat ctccatggtg ctcctgctgc tgtgcagcgc tcaagtgctc acactcaaat 120 gctacacctg cgatggggag aatgacaaaa tctgtaagac ggagaccgtc tgcccccctc 180 ttaaagatta ttgcaggacc taccagaagg gtgatgtgtt ttcccgaagc tgtgagagcc 240 tctgcgcaga ggactacttc accacctgct gcaccaccga tctctgctga tctaaccttt 300 taaaatcaag ataaatcaat gattatctct tccatttagt tcgtcttggg tgtggaagcc 360 aaatctggca atctttatct tttttttcca cctttgatca aaatgaaaca cagaaagtgg 420 acagttgagt ccttttcatc gtcccacagc ctggcttttt atccctcagg ccgggtgggt 480 gtttcaaaag cgggacaccc actcgcttac actcttttaa tccgctcctg atgtcactta 540 ttgggtcaca gctgcagtct tgtgctatgt gggtcactgt ctataagttg tactcctgct 600 ctcatctgtg ttaaactcat taaagaaaag tggaattaat ccttaaaaaa aaaaaaaaaa 660 aaaaggccgc ctcggccact ctgcgttgat accactg 697 <210> 13 <211> 653 <212> DNA <213> Oryzias sp. <400> 13 aagcagtggt atcaacgcag agtggccatt acggccgggg tggaggttca gacgccatga 60 agttcttagt gcttgccttg tttgttgctg gtgcctacgg gtgcggcttg cccacctttc 120 cccccatctt gaccagagtg gttggaggac aggacgtcag gcctaacagt tggccatggc 180 agatttccct gcagtataac aacaatggtg agtggagaca cacctgtgga ggtactctga 240 tttctgacca atgggtgctc actgctgctc attgtatcag ttctggaagg gagtacagag 300 ttgccctggg gaagcacaac ctggtggaga cagaggaggg ctctgtcttt aagagcacag 360 ccaacatcat tgtccatgag aactggagcg ccctgttcat tcgaaatgac attgccctga 420 tcaagctgga ttcccctgtt gagttctcag actcaatcat ggctgcttgt ctccccgtgg 480 ctggctttct ccttccccac aatgaatcct gcttcgtcac tgggtggggt cgcatctaca 540 ctggaggtcc cattgcagac atcctgcagc aggctctcct gcctgtggtg gaccacgcga 600 cctgcagcca ggcagactgg tggggcttcc aggtgacaga gagcatggtc tgt 653 <210> 14 <211> 392 <212> DNA <213> Oryzias sp. <400> 14 ttgccaatgg atacgacatt gctcttctga gactttcctc tgacgccgcc ctgaactctt 60 acgtccagct ggcttctctg cctccctctg ggcagattct gcctcacaac aatctctgtt 120 acatcactgg atggggactc acctctactg gtggaagcct gtcctctaag ctgaaggagg 180 cctatcttcc cttggtcgac cacaacacct gctcccgcag tgactggtgg ggcagcactg 240 tgaagaccac catggtgtgt ggaggaggtg gttctgagtc tggatgcaac ggtgactccg 300 gtggccctct gaactgccag gttagcggaa aatactacgt tcatggtatt gctagctttg 360 tgtctggata tggatgcaac tacccccaga ag 392 <210> 15 <211> 495 <212> DNA <213> Oryzias sp. <400> 15 aaatgcacag taccagaaaa catgaggcac cgtttcaggc gacacatgac aaaaagaaca 60 attaaaatca atatcttttt ttaaactttg taaaagataa catttaagaa taattttaaa 120 ggaaatttcc ttaatcttat tagaaacgaa atatttatgg ggcaatgtcc aaactttatc 180 ccactgaatc ttataaccaa atgtattcca ataggagata acgtggggct tagtgataat 240 ctcatttaaa aataatgaac gaatattttt tgttattttt accttagaaa aacagatttt 300 tccacaatat gaatctgtta atgcgggtgg agaacatggt aagttaaaag aggaagtaca 360 gcaaatcaac ttgacaccac taggaataga tctgatgact aatgcaaact cttcagaaga 420 gacagataga ttatatgtgg aaagaaattc gctataagtc attaaatttc catgtggagt 480 tatcagttgc gttac 495 <210> 16 <211> 162 <212> DNA <213> Oryzias sp. <400> 16 catgctgatc aagctgagca cgcccgccag cctgaacagc tatgtgagca ccgtgtctct 60 gccctccagc tgtgcttctg ccggcacctc ctgcctgatc tctggatggg gaaacaccag 120 cggcagtgga agcaactacc ccgaccgcct gatgtgcctg ga 162 <210> 17 <211> 565 <212> DNA <213> Oryzias sp. <400> 17 aagcagtggt atcaacgcag agtgccatta cggccgggga gatgatgcag gcagcgctgc 60 cggcgggact gaaacgatgg tgatccaggt gatcgtgaac acctttgagg gccagaagat 120 aaccgtggac ctgtgtcaag atgagaaaca gctgcagctg atgaccgtgc tgcaactgaa 180 gcagaaaata gcgctaaagc ttcccgggag agcagagcgg atggcggcag acatgcagct 240 gatcttcgcg gataagaggc tggacaagac agcaaactgc tgtgtgaatt cgggatcgtc 300 cacaagtctc tgatccagat ggtgatgtct ctggatggag gaagaggagc atgaccgcag 360 gaaaccagac atccagctgc acattcatca tgcatcaaac agactgttta cttgtttctt 420 ttatgcttta attgccttga tattgttgtc aaattctccc cagttggagt ttgttgcact 480 aataaaagtg gaaaacttag aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 540 aaaaaaaaaa aaaaaaaaaa aaaaa 565 <210> 18 <211> 922 <212> DNA <213> Oryzias sp. <400> 18 aagcagtggt atcaacgcag agtggccatt acggccgggg ggaaaagact ccgaagacaa 60 actgcgcagt tgaatctcca acatgtggac cctcggttct gttctgttct cggttctggt 120 cggttcatct gtttctggat cttcagatct gatcatctct gctgaacctg gacagaacat 180 cactctacca tgtggagatc caaacatcaa cgaggttttt cttttagagt ggatcagaac 240 tgatctgaag aaggatgaat atgtattttt atacagggat aaccatactt atcctgatga 300 tcaacatgaa tctttcaaga atcgcgtgtt tctgaagaac aatcagatga aagatgaaga 360 tctgtctgtg gttctgaaga acctgaagac ggaagattct ggaagatatg aatgtagagt 420 tgttaaagaa gatgatccac agagagaact gaagctgatc tccagcatcc atctgcaggt 480 gtctcctcca gatctgaaca tcactgctaa acctggagag aacgtcactc taccatgtgg 540 agatgcaaag atcaacaagg attcagtttt ggagtggacc agaactgatc tgcagaagga 600 taaagatgtg ttttttagaa tgaacaactc tgcagtttct gaaggtcgac ctgaatcttt 660 caagaatcga gtgattctga agaagaatca ggtgaaggat ggagatctgt ctgtggttct 720 gaagaacctg aagattgaag attctggaac atatgaatgt aaagatatga agagaagaag 780 caggagatca gctgatcaac gtcctccgat cgccatcatc aacctgctgg tgtctcctgg 840 agagaaagaa ggacaacaca aaggaggagg aggagatgga ggcagtcgtg gtcatcttgg 900 tctgattgct gcatcagttt ct 922 <210> 19 <211> 982 <212> DNA <213> Oryzias sp. <400> 19 aagcagtggt atcaacgcag agtggccatt acggccgggg actacagttt gaggacatgg 60 attccctcag gttaagcgtt gccatccttc tggctgtttc cacctgctcc tgggctgcag 120 agaaggaact gtccgtctct gagctcctgt ggaaggccaa caaggatgtc gtgcgttcag 180 gagacgagcc ctttgtggtg gatgacattg cttatgtcag taaatctgac aggaacgctg 240 accagtgcac gtccggcagc tgcttgtggc ccaaatccgc tgatggaagg gtctacatac 300 cctatgtgat cgctaaccat tacacttcta gtgagctcca agtcattgag cgcggcctgc 360 agtcgttctc tgaggtttcc tgcatccggt ttatccctta ttctggccac agagactaca 420 tccacatcca gtcacagaac ggctgttact cctacgtcgg acgcatcggc tacggtcagg 480 cgctgtcgtt gagccgtcag ggctgcatct accacagcac ggttcagcac gaactgctcc 540 acgccctcgg cttccaccac gagcaatgtc gttctgacag ggaccagtac atccgagtcc 600 tgtgggagaa catccagtcc ggtctggagt acgcctttga taaactcaac actgtgaacc 660 tggacacccc ctacgactac aactctgtca tgcagtacca cagatatgcc ttctctgcaa 720 acaacaagcc caccatggag ccctatcctg atcctaacgt tccttttggc acggctaccc 780 agatgagcaa caacgacatc acaagactga acaggctgta caaatgttga gccgtaagaa 840 caagtttgta cagcaagtcc tcaaagtacc gtggaaaggt ttccagatac aaataaatca 900 ataaaggaaa aataagtttg atggttgtag agctcagact ttaaccgcat catgactaaa 960 taaatgtaat acaaagctct ag 982 <210> 20 <211> 334 <212> DNA <213> Oryzias sp. <400> 20 gcagtggtat caacgcagag tagccattac ggccggggat gagtgtgaaa caagaaactt 60 taatcacgac aaagaccgaa atctctgatc tgagacgatc attacaaagc ctggaaatag 120 agctgcagtc tcaactcagt ttgaaatctg caatggaggg cacattgtca gaaacagaag 180 cgcgcttttc cagtcaacca tgagcctcca gcaacaggtg acaagtctag aagaacaatt 240 agtcgtggtg cgcactgaca cggaacgcca acgtcaagaa taccagatgt tgttggacat 300 caggaccagg ctggagatgg agatcgcaga atac 334 <210> 21 <211> 337 <212> DNA <213> Oryzias sp. <400> 21 aagcagtggt atcaacgcag agtgccatta cggccggggc ccccaacgac attgccctca 60 tcaagctgtc cagccccgcc aaactgaacg cccacgtgtc ccccgtctgc cttgctgagt 120 ccgccgattt cttcgcccct ggctcaacct gcgtcacctc tggctggggc ctgacccact 180 acctggctcc cagcactccc aacaagctgc agcaggcctc cctgcccctt ctgtccaacg 240 acgactgcaa gcgctactgg ggcagcaaca tctctgacgt catggtctgt gctggaggag 300 ctggtgccac ttcctgcatg ggagactctg gcggtcc 337 <210> 22 <211> 391 <212> DNA <213> Oryzias sp. <400> 22 atgcaaactc cagccccagt tatgccttca ttgataactt tggatgtatg cttgacggca 60 gaatcacagg ttctgactca aagttcgtgc ctcgaccagc tgagaacaag cttaacttcc 120 agttggaagc tttctggttc cagggtgctg acagcggaat gatctacatc acctgccatg 180 tgaaggcgac atctgctgct tatccccttg acgctgaaca caagcttgct cttacgtcaa 240 tggatggagg gaggtcagtg gtgcagatcc agtttacgcc ccctgtgagt cgagtggatt 300 tacggctcat accaatacag cagtatcacc tggcacctca attattagtg gaggcggtca 360 cggatctgga aaaccttcag actcttcaag g 391 <210> 23 <211> 670 <212> DNA <213> Oryzias sp. <400> 23 aagcagtggt atcaacgcag agtgcgagga gatgctgctc cacgcttttg agggttacaa 60 cgtttgcata tttgcttacg gacaaacggg agcgggcaaa agctacacaa tgatgggccg 120 acaggaacag gaccagcagg ggatcatacc cctgctgtgc gaagaccttt tcacaaagat 180 aaacgatagc aataatgaca acaacatgtc gtactctgtg gaggtgagct acatggagat 240 ctactgtgag cgtgtgcgtg accttctcaa ccccaaaaac aaaggcaacc tgcgagtcag 300 agagcaccct ctgctggggc cgtacgttga ggatctgtcc aaactggccg tcacctccta 360 caatgacatc caggacctga tggactctgg gaacaaggcc aggactgtgg ctgcaacaaa 420 catgaacgag accagcagcc gctctcatgc tgtctttaac atcatcttca cacagaagaa 480 atacgacatg gagacggaca acacgtcaga gaaggtcagt aagattagtc tggtggattt 540 ggctggcagt gagagagcag actcaactgg agccaaagga accagactga aggaaggagc 600 aaatattaat aaatccctga ccacactggg gaaagtcatt tctgctctgg ctgaaattga 660 ctctgctcca 670 <210> 24 <211> 829 <212> DNA <213> Oryzias sp. <400> 24 aagcagtggt atcaacgcag agtgaccatt acggccggga gggatctgtc tgaacagatc 60 aagaaagtta caaaggagag tcatgtcaga gcagaaaaca ccgagctcat gctcagcttc 120 cagcggggac aagtcacact tccacagtat aagctcctcc tgtgttccct gtacgagatt 180 tacaaagctc tggaggaaga gctggacagg aactccaacc atgctggtgt tgcacccatt 240 tactttccat ctgaactggc cagactggag tccattgaga aagacctgga gtatttctac 300 agcaccaact ggagggagaa gatcgttgtt cctgctgcta cgaagagata ctgtcacagg 360 atcagacaga ttggcaaaga gaacccagag tttctggtgg cccacgctta cacccgttac 420 ctcggcgatc tgtccggagg ccaggttctt ggtcggatcg cccagaaatc catggggctg 480 aaaaccagcg agggtctgtc tttcttcgcc ttccctggag tctccagccc caacctgttc 540 aagcagctct accgcagccg aatgaacagc gtggagctga cggaggagca gaggaacggc 600 gtgctggagg aggcggtccg gtccttcgag ttcaacatcc aggtctttga tgacatccag 660 aagatgctgg acgtcacaga aaacaagctg cagaacagtt taacagacaa gtcaagagag 720 gctaaaacgc tccacgtccc gctcatcagg atggtgctgg gactttttgt ggctctggct 780 acagtcagca tgggattcta cgctctataa aattagactt ttttttttt 829 <210> 25 <211> 1396 <212> DNA <213> Oryzias sp. <400> 25 aagcagtggt atcaacgcag agtggccatt acggccgggg agaagcttcg ggacgttgga 60 acaggatgaa gtctgtttgg ttgctgttgg tgcttgtagc tgcagtcaaa gcggagagga 120 cttttgttgg tgatcaggtt ttcaggatcc atttgcagtc tcaggaacag ctccgtgttc 180 tccagtctct ggaacatgaa gaggaatggc agctggactt ctggctccac cccgtttcca 240 cagacctgcc agtggatatc cgagttccca gctccagtct gagctccatg aaggagttcc 300 tcctcaccca taacatcccc ttcaccatca tcattgagaa ccttcaggag cttattgata 360 tggagaaaac tgaaatggag gagagccaaa aaatggagaa gagcaccagg agcttcaact 420 ttggagctta tcatactctg gagacaatct acagctggat ggacacactg gtggctcaat 480 acccacactt ggtcaccaaa caagaaatcg gtaaatccta tgagaacagg cccatgtacg 540 tcctgaagtt cagcactggc ggcaccaacc gccccgccat ctggattgac acggggatcc 600 actccagaga atgggtgtct cccgctacag gactgtggac ggctaacaag attgtctctg 660 atttcggcag tgacgcctct ctgacctccc ttttaaacac catggacatt tacatgctga 720 tcctggccaa tcctgatggc tatgtgtaca cccacactaa taaccgaatg tggcgcaaga 780 cccgctccct aaactctggc tcatcgtgtc gtggagtaga tcccaacaga aactgggatg 840 caggcttcgg tggtcctggt gccagtacaa gcccctgctc tgattcctat cacggaccct 900 ttgctcactc tgagattgaa gtgaagaacg tggtggattt gatcaagagc cacggcaatt 960 ttaagtcgtt catctccgtc catgcctact cccagctgct catgtatccc tatggatata 1020 cctgcacaga tgtggcagac aaggctgagc tggattctct tggcagagca gccacacaga 1080 aactcacctc cctctatggc acctattaca aagttggaaa catttgcaac attatctatc 1140 cagcaagcgg aggcagcatt gactggtcct atgatacagg catcaaatac tcgtttgcct 1200 ttgagctgcg ggacaccggc tactatggct tcctcttacc atccaatcag atcatcccca 1260 cggccacaga gacgtggctt gctctaaaac acatcatgga gtatgtgcgt gatcacccgt 1320 actaatggct gcatagtcat ctgtgaattt tagattcagc tttccctctg tgaccaatta 1380 aagtcatcaa aaccct 1396 <210> 26 <211> 576 <212> DNA <213> Oryzias sp. <400> 26 aagcagtggt atcaacgcag agtggccatt acggccgggg acatccgtcc ttctccgtca 60 tacctttacc cccccagagg cgtctcatcc gcagccatga agttctcctg tgttgccgct 120 cttcttgtgg tgctggccgt cactcgtggg tgtgaggccg tgtccctggt gaggcgggac 180 gccccgtccg aggtggacag gctcacccag ctgatggaga acatgtccgc cagcatcgcc 240 gccgccaccc aggaggtggt ggacaagatg aaggcgctcg agatgaccag cgccgcccat 300 gtttccaagc tccagcccct ggtggagcag gtccaggccg aggccgccaa gctgcaggag 360 caggtcaaac cctacgtgtc caacatcgag gagcacatcc gacccctgac cgacaacctc 420 aacagccacg tcaagcctct caccgacatg atggagaagc ttttgcagca ggtggtggac 480 cagtccaaag ccctgctgcc cccccagtag acctgtgtct gttttcttct gtctgtaatt 540 tatctaaccg tgtttgaatt aaaggcttca aaagtt 576 <210> 27 <211> 1486 <212> DNA <213> Oryzias sp. <400> 27 aagcagtggt atcaacgcag agtggccatt acggccgggg actgccaaca tgaaggtcct 60 gctgtttttt ggactggtgg ctcttgccct ggctgacgtc actcgatttg aaggagaaaa 120 agtcctccgt ctgaagcctg tcctcgctga acatgtgacc ctcatcaagg atctggctga 180 gagcatcgaa gtggacttct ggagccctga cagtcctgag ctggtgacta tcgacatcga 240 tgtggacatc cgtgtgcccg ccaagtacct ctccctggtg tacaccaccg tgggtcagag 300 cggcatggag catgagactc tcatcgatga cgttcaggtt gccatcaacc gccaggctga 360 caacgggatc agtcctagaa atctgcacag ctacaccaag tacaacactt gggacactat 420 cctatcctgg atcaacacca tcgcctcctc caaccccagc ctgatcagca agcaggagat 480 cggaaacacg tttgaaggac gccccatgac tctcctcaag attggcaaat cctctggcgc 540 tactaagccg gccatcttta tggactgcgg catccacgcc agagagtgga tctctcctgc 600 tttctgccag tggtttgtga aggaggcttt ggagacctat ggcagtgact ctcagatgac 660 cagcctgctt gaccagatgg acatctacgt cctgcccgta ttcaacatcg acggctacga 720 atactcacac aactctaaca ggatgtggag aaagacccgc tccaagcttt ctggaagcag 780 ctgccttgga gctgatccca acaggaactt tgatgccggc tggtgcaccg tcggagcctc 840 cagtaacccc tgcagcgaca ccttctgtgg ctccaccccc gagtctgaga tcgaggtcaa 900 gaacgtggct aacttcatcc gcaagtacaa gtcctccatc aaagcctacc tcaccgtcca 960 ctcctactcc cagctccttc tcttccccta ctcctactcc tacaacctgg ctgccgacca 1020 cagtgagctg atgagtgttg ctcagggagc ctctgctgct ctgactagtc tgtatggcac 1080 ccgctatacc agtgggcctg gtgcttctac tatctatctt gctgcaggag gttctgatga 1140 ctgggcctat gacctggggg tgaaatattc ctataccttt gagttgcgtg acactggtta 1200 ttatggcttc ctgctgccag agtctcagat caagcccaca tgcgaggaga ccatgctggc 1260 cgtcaagtac attgctgccc atgtgcagaa gaacctctat taaaaagcgt agcagtcagg 1320 acccctgcca acgcaatgcc tctgccccag cccccgagcc cctccaacct ctcagccgtc 1380 tcatgcccgt atctatggat acgggcgcca cccgccatgt tttctcactg tgcttgacag 1440 agtgtcacga tgcaagtgca aacaataaaa tcaatgatcc atcccc 1486 <210> 28 <211> 426 <212> DNA <213> Oryzias sp. <400> 28 taaagtatgc cgtacctgat cctctaaaag aggagaacag ggacacttta gtccccgttg 60 aacaagcaaa cacacatttc ctctttgtag cctcagaaga tgatctcagt ttggacagta 120 gagctttcat gatagagatg atggagagat tgcagcgtct tgggaagaaa aactttgaca 180 gtgtgtgtta tcctaaagct ggacactttc tagagccccc ttatggccca ttctgcccct 240 ccagcttaaa caggtttgta aaaaacatat ctttgtgggg gggagaatca agggcccatg 300 cagcagctga agttgacatg tggaagaaga tccaagagtt cctgaaaagt catgtgagct 360 gtgatcctgt gcactttgat gccaaattat agatttaatg tgagtgttaa taatcataac 420 tatcaa 426 <210> 29 <211> 844 <212> DNA <213> Oryzias sp. <400> 29 aagcagtggt atcaacgcag agtggccatt acggccgggg acatttcaac acgtgctctg 60 ttctgtgttg cacctctatc aaaaaagatc cattatggca actgtggtgg tggtgaatca 120 aggcccggga aactggagca ccgggttgtg cagttgctgc tctgatatgt ctacctgttg 180 ctgctccttg tggtgcttcc cctgcatgca gtgtcagacg gccagccgcc atggctggtg 240 ctgcgccatg ccgctcctgg atgtgttctg cggcgtggtg acctgcgttc tgcagtcctc 300 catcagaaag cgcccaacat ccctggttcg tgctgcgagg actgcctcaa gtgctcgttc 360 tgctacgtgt gcgtctggtg ccagatgaac cgagaggtga agatccgatc caagaccgtc 420 agccccaccg tggtcaccac gcaggtcgcc agagcttagc cggctggagt taaccattcg 480 tttaaggaaa cgccttaaag gggttcaaga aatccataat gtaactgttt ttgttggtat 540 attaaatgtt ttcagtttgt taaagctaca ggaaatgttg taataataat ctttggtttg 600 actccaaatg tttttttctt gcatccagaa aaaaatatga ctttaagttt gtaaacagaa 660 aaaatatctt aaattacata caaattgatt ttgtttatag aaatggtttt atatgtgagt 720 cacggcctgt tttgatggat ccatttaaaa ctgttcgtaa aaggctttcc taacatacgg 780 ttctgtgaaa aaacatttcc cttttcatat ttttatttgt cttaaataaa aagctttgag 840 tcaa 844 <210> 30 <211> 532 <212> DNA <213> Oryzias sp. <400> 30 tgtctccgac gactaagcag tggtatcaac gcagagtgcc attacggccg gggacatctc 60 ctcagatttc ttttctctgc acaccaaaaa ccaagccaaa accatgagtc agctggagac 120 ggccatggcc atgctgatgc agaccttcga cacgtacgcc ggcaaagatg gcaagaagga 180 atcgatgagc aagacagaag tgaagaccat gttggagaag gagctccccg cactcctgaa 240 ggcagcaaag aaccaagctg aggtggacaa gctgctgaag ggcctggact tcaacggaga 300 ctcggaggtg gacttcagcg agttcgtggt gatggtcgcc gctctgacgt gcgctgcgca 360 cggccgctgc tccaagtaga actgagctgc gcccttgggc gggacacgga gacgtcaaca 420 acatgaaatc ctcacggttg cttttgtaac gacagaataa agtgatttac ctacaaaaaa 480 aaaaaaaaaa aaaaaaggcc gcctcggcca ctctgcgttg ataccactgc tt 532 <210> 31 <211> 427 <212> DNA <213> Oryzias sp. <400> 31 aagcagtggt atcaacgcag agtgcgccag tcagccttgt cttaacaacg gcaaatgcat 60 cgacaaaatc aactccttct actgcgagtg ccccaaaggg ttttcaggaa atctgtgtca 120 ggtggatata gatgagtgtg ctagtacgcc ctgcaggaat ggagccaaat gtacagatgg 180 tcccaacaag tataattgtg aatgtgctga aggtatgata tgatggaatt ttgtcaaaag 240 ttgcttgtaa cttttcgttt tcctttgagg gcatgaaaat cttttcatat gtgtgtggag 300 aatttcaaaa ggcatattga catttttgca aaaaatcaaa tttttgtaac acaaatttca 360 tgtttgagct aagagtctca aatgtgtatt aaagggtaac aaataaagat caatttgtga 420 caaaact 427 <210> 32 <211> 701 <212> DNA <213> Oryzias sp. <400> 32 cgttgttggc aaagtctaac ttaacactgg cgtggttgat tcctacaaag acggcgatgc 60 atctcatgac gctggcccac tccctcttga acttgtgggg ctctcccaga tgactgtcga 120 ggcaggggta cagcaggccg ataacagctg ctcctgtccc gcagcaaggt gggatccacc 180 aggcggagga gaacaacgtt gtcatcacct cctcgggaaa cagagtgacg tttctttgta 240 tctgtagtaa attgagcacc agagccagga agaccccgac cgtgaagagg accagacccc 300 ggcggatgag gttggtggtc cggacgtcgt gcagagaccc gtaagcgctc ccgagcaccg 360 tggttatgat ggacatcatc tcctcagcct tggatgcaaa ccagctcgcc ccagatgaat 420 gcttagcgtc agccttggga gaacaagagc agctccagca gtggtcctcc agtctcggca 480 tttggcactt tttcacttga tttctcttca gactgtgacc caaacttccg tcctgctcgt 540 tggcgcctcg gccagtgatc aaactatcgc tctgttttgt tttcccgcac tctacgacaa 600 ggactgcggt cacgttgttt gctcacatca tcgtttcagg aacaaaaaaa aagaaaagaa 660 agagaaaaca cacacacaca cacacagggt tgcttcattc c 701 <210> 33 <211> 440 <212> DNA <213> Oryzias sp. <400> 33 ggatccacca agccagttcc catcaaccct gaccagacgt gtggagatgg atgggtgtgt 60 gagcacagat ggcgtcagat tacgaacatg gtccaattcc gtaatgtggt cagtggacag 120 cctcatgcca actggtggga caacggcaac aatcaggtcg cctttggccg cggaaaccgt 180 ggattcatcg tgttcaacaa tgacgactgg gctctggatg tgacgctgaa cactggcctt 240 cctggcggca cctactgtga cgtcatctct ggcaataaga acggaggcag ctgcagtggg 300 aagcagatca ctgttggggg tgatggccgt gcacacttct acatcaacaa ctcagaagaa 360 gaccctttca ttgctattca tgctgactct aagctgtaaa tgaaaaccgg agacaataaa 420 acctgcaaac taaaaaaaaa 440 <210> 34 <211> 659 <212> DNA <213> Oryzias sp. <400> 34 aagcagtggt atcaacgcag agtgccatta cggccgggga cagaacctga gccagaaccc 60 ggaccggcac aagacgcccg acccagatcc agtatgcagc acgctcgtgg cgccgtcttc 120 gtggttctcg ccgcgctggc tctcagcagc gtgggagtat cctcgcagtc tgacagagac 180 caggaccagt tccagaacca ggacctggac ctggagctgc gtcaccaccg gctgctgcag 240 cgagctcgca gcgccgggct gctgtcacag gactggagta aacgggccat cgagaacatg 300 ctggcccgca tgtcggaccc cgaggccgaa cctgagcggg aggccgaggt gatctccatg 360 acctcagggg ggcggggcaa cctggagcgc tcggtggaca ccgtgccccc ccgccaaccg 420 caaagatggc tgcaaaaact tctactggaa aggcctcact tcctgctgat gccccgccca 480 gctgaggctc cgcctccccg acgaccaaca gacccgtcct gatgtcctga cagagaagac 540 caatcagcag atgacctgaa taattaatgt aattatccaa taaagagaga aatacaatac 600 agaaaaaaaa naaaaaaaaa aaaggccgcc tcggccactc tgcgttgata ccactgctt 659 <210> 35 <211> 146 <212> DNA <213> Oryzias sp. <400> 35 caatcagcta ggaactgtgc tgagtcatat ttaggggcgt tctggtacaa aaagtgtcac 60 agcacgaacc ctaacggcgt ttatcgttgg gggagtgatg ctgcctttcg tgggattgga 120 gtggattggt ccacctggaa gggtca 146 <210> 36 <211> 955 <212> DNA <213> Oryzias sp. <400> 36 aagcagtggt atcaacgcag agtggccatt acggccgggg gcagaagcat caatcaacat 60 gatccccatt gtgctggcct cagtcctcat cgctagtgcc cttgggtgcg gcaccccacc 120 cattcaaccc ctcacttccc gcgtggtcaa cggagaggat gccaagcccc acagctggcc 180 ctggcagatc tccctgcagt acgagaggga tggagagtgg aggcacactt gtgggggaac 240 tctgatcgct gccaactggg tgatgactgc tgctcattgc atcaactccg acttctctta 300 cagagtgttt gtaggtaaac acaatctgct ggaggacgaa gtcggttcca aagccatttt 360 cccagagaag attgtcgtcc atgagaactg gaactccttt ttggtggctt taggtaacga 420 cattgccctg attaaactgt ccgagcctgt gactttgagt gatcatgtgc agctggcatg 480 tattccacct gctgagacgg tgctgtccaa cctctacccc tgctacatca ctggatgggg 540 caggctgtac actggaggcc ccattgctga taacttgcag caagctttga tgcccgtggc 600 cgaccacgcc acctgctccc agcccgactg gtggggtttc attgtcagga ccaacatggt 660 gtgcgccggc ggtgatggaa ttgtggctgg atgcaatggt gactcaggcg gcccgctgaa 720 ctgtcaaaat gctcagggtg tctgggaggt gcacggcatt gccagctttg tgtccggtct 780 tggctgcaac tttgagaaga agccaactgt cttcaccaga gtctctgctt tcaatggctg 840 gatcgaccag gttatgataa ataactgaag aaactgaata aatatgccca gaaaaccaca 900 aaaaaaaana aaaaaaaaag gccgcctcgg ccactctgcg ttgataccac tgctt 955 <210> 37 <211> 342 <212> DNA <213> Oryzias sp. <400> 37 gaaggataca aactggaggt gacgggattc actgatggag gagcaggaga ctctctgggt 60 taccacaatg gaaggaagtt ctccaccttt gacaaagacc aggacgtttc atcccagaac 120 tgtgctgagt tatatttagg ggcgttctgg tacaaacagt gtcactacac aaaccctaac 180 ggcgtttatc gctgggggag tgatgctgcc attcgtgcga ttggagtgga gtggtacacc 240 tggaagggat atgactattc tctgaagaga atcagcttca agatccgtcc tgtgaaataa 300 atctgcagga gaaccgttca acaggtccgt ctgtgtggtt cc 342 <210> 38 <211> 146 <212> DNA <213> Oryzias sp. <400> 38 caatcagcta ggaactgtgc tgagtcatat ttaggggcgt tctggtacaa aaagtgtcac 60 agcacgaacc ctaacggcgt ttatcgttgg gggagtgatg ctgcctttcg tgggattgga 120 gtggattggt ccacctggaa gggtca 146 <210> 39 <211> 1215 <212> DNA <213> Oryzias sp. <400> 39 aagcagtggt atcaacgcag agtggccatt acggccgggg acacctccca agtggtacaa 60 cgcacacaca ctcggaggat ttgatgatct gaccttaaga gcagagatat atatcaccag 120 ttagaccatc taatagacct ggatctactg tttcgggaca tcgaggggtc atggatccga 180 tcgtggaagt ggtggctctg attttaggct tcaccgggtg ggtgatggtg ggagtcgccc 240 tccccaaccg ttactggaag gtctcgaccg tggacgggaa tgtcatcacc acctccacca 300 tctacgagaa cctgtggatg tcctgcgcca cggattcgac cggggtgcac aactgcaggg 360 agttcccgtc gctactggct ttaaatggtt acatccaagc ttctcgcgcc ttgatgatcg 420 tgtccatagt gatgggaaca ttcggtctgg tgggggccct cataggaatc cagtgctcca 480 aagcaggggg ggaaaactat cttctcaaag ggaggattgc tggcactgct ggagtcttct 540 ttatacttca aggtttgtgc accatggtgt cggtgtcctg gtatgccttc aacatcacgc 600 aggatttctt tgaccctttc tatccgggga taaggtatga aatcggagaa gggctttaca 660 tcggctggtg ctccggagtg ctcgccatcg ccgggggagt ctgcctcacg tgttcctgca 720 aagtggcgag agaggaaaaa tacaatatcc cgtatcaagc agaggaacgg tttactccgg 780 tgcagcaccg accagaagtg tagtcgccag cacttacgga agaaacgctt acgtctgaag 840 gacacaagag cggggacatt ttcctacgtt tttctttttt tgttgttgtt gctgatgcat 900 gttgtgggtt taaattggaa cttttggtcc atgacacaac tggaaagaac caagcacaac 960 tgcacgtgtt tgacttcaat acaaggacgt ccttttgtaa acattgtaca aagcccacta 1020 cttttcctga atgaatgttt tcgtttttaa ttgatagtgt acgctgaaaa aaaacatata 1080 atcatttgat gatctgagct gaaatgacca gaagtatgaa caaagtcaaa attattcaaa 1140 tgttattttt gtgtgttttt tgtacagttt tgtaaagttc tttaagcaca ataaagggag 1200 ggtttttttg ttgaa 1215 <210> 40 <211> 841 <212> DNA <213> Oryzias sp. <400> 40 gtggctccag cgaggatggc catgccaatg aacagcctgt ctctggtgtc agacttcagt 60 gaagatacta agactccttt gattaaattg caggacacag ctgtggtcag cgtggagaag 120 cagctgatgg atctcatgca ggatgtcagt ctgccaaagt tcaatgagac cttcctggcc 180 aacctgcaga ccctgaggca gcagatcaat gcagcgagtg gcagaccttt gagtcttgga 240 tgcgcaactg gctgatcttc cagatggctc agaagaagcc tcctgctcca actccccagc 300 cagcatccat gatcaagacc aaatgccagc tggaggcagc accggacacc atcgccaaga 360 tcggcacgta caaacctcag tgtgacgagc agggcaaata caagcccatg cagtgctggc 420 acgccaccgg gtactgctgg tgtgtggacg agtctggcaa ccccatcgag gggaccacca 480 tgcgcggtcg ccctgactgc cgtcgaggct ttgcacctta tcgcaagatg gttgcaccca 540 gaatgatgca gaggacttat cttgaggatg atgctctcac aaaagacgag tgaatgaagc 600 cgagccgagc cgaagcgagg agtttcttct cctgatcctg tctttgcaga actgtatatc 660 ctattattgt aaatctgagg cagatctgac aatttgctgt tcaaatcacg ccacctgtcc 720 atgttatcac ttgattttaa tttgctggct gcttcaaaac aatcatgaca aagcttttac 780 gcttctaatg tcttacttct gtcacctttc acagactttt ctgggtgttt ccacttaatt 840 t 841 <210> 41 <211> 1489 <212> DNA <213> Oryzias sp. <400> 41 aagcagtggt atcaacgcag agtggccatt acggccgggg gacaaacaga tagggcggtg 60 aagtggtcga aagcacacca gtggatggtt tgtaacttac acaaagggag gactttactg 120 tgactgtggg aggaagctga ccgcaaaaat gtcaaacaaa atgatcgagc ttgaggagaa 180 tgcgacccac acaggtccaa aaggagtcat caatgactgg aggaggttta aactggaaag 240 tatggaccag gaaaacttgc cttctgcaaa aaaagaactg ctgagacaaa tgtcttcccc 300 caataaatca aaagatgact ccagagcaaa cctaaaccgc aagatgagtg tccaagagta 360 tgagctgttg aaggaagagg atgaagggtg tctgaagaag tacagaaggc aatgcatgga 420 agaaatgcac aacaagctca gcttcgggcc caagtttgaa ggcgtacacg atctggacag 480 tggagaggcc ttcctagaag tcatagagaa ggagcatcac agcacggtgg tggttgtcca 540 catctataag attggtatca aaggttgtga gcagctcaac agctgccttg actgcctggc 600 aactgagtac cccaccgtaa agttctgcag gatcgacgct gttgcgtctg gagccgctga 660 gcgcttctcg gatgaatatc tacctactct gctggtgtac aaagcaggag agctactagg 720 gaatttcctc tcctgcacac agcacctaag tgaagaattc tttgccacag acgtggaagc 780 ctttctcaat agctatggtc tgctgccaga aaaagagctg cccaagctgg aggatgaaga 840 agaaaatgat gtagagtaaa tcaagtgtgg ccactgtcag tgaaaagaag cagtgtcaaa 900 gatccaagac tgggaagatt catacataaa atatgatgag tattctttat tgccttagca 960 aataggaaat ccatatcatc ttctctagaa ctctagttct ggattattct ctaaaggaga 1020 cacaacaatg gcagccttaa aacaccaaat atgggagctg ctaattgact gaaggataac 1080 aaaacaacta aaacagtttt attttttaat tttgtaattt tagataatgt tacatactgt 1140 gtctttgtaa aaaggattca tacttatctt tttttagtct tagtttatag atatttaata 1200 ttgaaaatga ttttaaaata gtttgatata ttgttcctaa attatcttga acgtatagta 1260 caattcccat gtttctatgg catcactgta atgttctgct agaacttaat acatgaagga 1320 ctgaaatgaa ccataaaaac gttgtttgat tgtatatgtt aaagtagtga agttgactgt 1380 tgtctctgga gaacatttat gagaaacaaa ttaatcaata aaacatgaca actgaggaac 1440 aaaaaaaaaa aaaggccgcc tcggccactc tgcgttgata ccactgctt 1489 <210> 42 <211> 848 <212> DNA <213> Oryzias sp. <400> 42 aagcagtggt atcaacgcag agtggccatt acggccgggg ggacagtcca ggatccgacc 60 tcaacaatgt tcgctccgct tttcgtgtgt gccctgggcc tgattgcggc ccctcaggtg 120 tttggggtct tggaagagtt tcaactcaaa gatgaggctg ttactcctta ctctccgagt 180 gagattgtga acaagcacaa tgacctgagg aggagcgttc agcccacagc cagcaacatg 240 atgaaaatga aatggaacac tgaagctgaa gccaatgcta agaagtgggc caatacctgc 300 tccatgaccc acagccccgc cagctccaga gaaatcagca ccagtggttg tggagagaac 360 ctttacatgt ccagtttcca gaactcgtgg agcaacgcga tccaggcctg gtacaacgag 420 gtgaaggatt tccagtatgg agtgggatcc atcaacggag gcgtggtcgg acattacacc 480 caggtcgttt ggcacaactc taaccagatc ggctgtgcca tggcttactg tcccaactcc 540 acctacaaat acttctatgt ctgccactac tgcccaccag gaaactacca gctgtctcgg 600 ccttacaagt cgggaccctc ctgcgccgac tgcccctccg cctgcgagaa caaattgtgc 660 ttgaggtagc tccgagggag cggggggacc gcacagtcac caaccgcaca gtcaccagga 720 tgggggcgtt tttgcgtcca atcagatcct ctgactccga ttcaataaag acatcaatcc 780 ttagaaaaaa aaaaaaaaaa aaaaaaaaaa aaggccgcct cggccactct gcgttgatac 840 cactgctt 848 <210> 43 <211> 473 <212> DNA <213> Oryzias sp. <400> 43 catggaagac tttgaaggaa acaaggcgta cgctcgctac tcctccttct ctgttggtcc 60 agaggttgaa ggatacaaac tggaggtgac gggattcact gatggaggag caggagactc 120 tctgggttac cacaatggaa tgaagttctc cacctttgac aaagaccagg acgttttatc 180 tagcaactgt gctgaattat atttaggggc gttctggtat agagattgtc accacacaaa 240 ccctaacggc gtttatcgtt gggggagtga tgctgccatt cgtgcgattg gagtggagtg 300 gtacacctgg aagggatatg actattccct gaagagaatc agcttcaaga tccgtcctgt 360 gaaataaatc tgcaggagaa acgactaaca ggtccgtctg tgtggaacat cgaaagaatc 420 attcagaaat ggaagagaga tcagatgatg atagagagga gaagcaaagg aat 473 <210> 44 <211> 146 <212> DNA <213> Oryzias sp. <400> 44 attgtatcta tcaactgtgc taagtcatat ttaggggcgt tctggtacaa aaagtgtcac 60 cgcacgaacc ctaacggcgt ttatcgttgg gggagtgatg ctgcctttcg tgggattgga 120 gtggagtggt acgcctggaa gggtaa 146 <210> 45 <211> 693 <212> DNA <213> Oryzias sp. <400> 45 tccagcagga tgaccacaaa ccaaagtcat tgagcaacta ttaaataagt tcaagatcga 60 gaatgatcca gaggagtttg ctctatattg cattcatcag agtggngaaa agaggaagct 120 gagcaacaaa gatctgccgc tgtgggagcg cgttctgtac gggccctctg atgacatcat 180 gaagatcttt ctgatggaca tgcatgaaga ggaagtcagc gatgatgttg ctcagtatct 240 gaacttggag cgttcaatac tggagcaggt tcttctgcag ctcagagagg aagaaaacag 300 agaaatagag agtgtcatca tcaagtacca gcaccagcac agacttctgt ctcaggtgct 360 gaactccaaa atgtcccctc acatcgagac cactgtgtga cagcaccatc catccatcca 420 tattgaaacc tgctcattca cggtcacagg gttgctggag cctatccagc tatggttggt 480 atcaacgcag agtatacccc gggctgtttg ctaatccatt gtagatggat taattactac 540 accacccttc aactcaagga acaatttgct tttttttttt tttcataaaa tgttaatttt 600 aaatttagat agttaaaaat aaagtttggt gttgttctac ttttgttaca tgctctttta 660 aacacatgtt gcttctgtat taagttttac att 693 <210> 46 <211> 1009 <212> DNA <213> Oryzias sp. <400> 46 aagcagtggt atcaacgcag agtggccatt acggccgggg acgtcagatt acaccttaca 60 ggtgctcgtg ctcatcatga gggttcttgc cgcgatcctc gctctggctg tcctctcagg 120 gtcccacgcc cgcagcgtgc ccctggagga cctgcagaac acgtgggagg tgaccgtgga 180 gaagttccag aattacatca cagacctgaa cacaaaggct gacggcgtgc tggagaacat 240 caggagctct cagatcagca gagagttgga aactctgatc caggacagca tgtcagagct 300 ggccatgtac agagacgacc tgcagaacaa gctgggcccc tacacccagc agagcgcgga 360 gcgcctgagc caggacctgc agctgatggc ggacaaactg cgcgagaaca tgaatgaggc 420 gcgcgagcag gtggaccgct acgtccagga gctgcagacc atgatggagc agaacgccga 480 cgacgtcaag ctcagggtgt ccacctacag ccgcaagctg aagaagcgcc tcaacaagga 540 cacgcaggag atcaagaggt acgtcagcga gtacgtcgag gagctccact ctcgcacctc 600 cgagaatgtg ggagagctga aagcccgcct ggatccgtac ttctctcagg tccgggacaa 660 cgcccaggcc aagatctcca ccctgaacga gctgctccag tcccagatcg agaacgtgaa 720 gaccagggtg cagaccaccc acgaggacat caaggagcgc gttaaggtcg ccaccgagga 780 cctgcgctct tctgtggagg gaaggattca ggaggtgcgc aactggttcc agccctacgt 840 gtccatggtc acggagagcc tgtagataag actggtgacg acccctcatg gaaacatgtt 900 cagactgaaa ccaacaagtc tgtggggaag catgaaatat taaaagatac taaaaacaaa 960 aaaaaaaaaa aaggccgacc tcggccactc tgcgttgata ccactgctt 1009 <210> 47 <211> 394 <212> DNA <213> Oryzias sp. <400> 47 tgctgtggat caacagtact gggagagtca aactcaagcc tctctgggtg cacagcagac 60 ctacaaagcc aacattgaaa tagcaaagca gcgcttcaac caaactggag gtgttcacgt 120 gttccagaac atgtacggat gtgagtggga tgatgagact ggagaggtca aaggctatga 180 tcagtatggt tatgatggag aagacttcat taatcttgac caaaaggaag aatcatgggt 240 tgctcctaaa caacaggctt tgattaccaa acagaagtgg gaccattcga gagcttttac 300 agcatctgaa aagcaatact taacacagga gtgtcctgag tggctgaaga aatatgtgga 360 ctctggaagg agttctctga tgaagaaagt gttt 394 <210> 48 <211> 828 <212> DNA <213> Oryzias sp. <400> 48 gaaggacatg tgggtggagc cggagttgct ggaggccctg aacgaagagc agaagaagat 60 cctcttcctg aagatgagag aggagcaggt gcgccgctgg accgagaggg aacagaggga 120 agagaaagaa ggacggagca acgcagagac caagaaagct acaagtaaac gcgtgtcgtg 180 gcttctcggc cgtgatgggg acgtgagcgt cagcgtgata ggagaggtgg acgaattcag 240 gtcctccaaa atcctgcaga acctccagac cagactccac actgataata tgaatggcat 300 ccagacggcg gacttgctgc caggaagaga ggcccagcag ctcagcttca aagacaacgt 360 caagctgccc ctcgcagatg tgaacgaaga accggtttcg tcgaacggcc aatcgtctga 420 ggagggtttt agtgccgacg gcgacctgaa agaccccgca aaggacggtc gcgtaacaga 480 gctaaagaag acgttcgcag accatcccca caacacatca cctgcccgcc ccagaccgct 540 catacctgac aaaccggctc acctacagag gacggcgcgc agttgacggc tgtaggactg 600 catccaccct gctgcaagac gacactcgac cctcacagag ccaaaggtta cattactgag 660 cgtgctgagc tccacgcaag cgtcacagct cgcttaggaa aacagccaaa gcatcgatga 720 actgaacatc actgcaagat ttgatgtcta gatctgacct gaatgggaag attccagatt 780 aaatttgagc tcaaacactt gcagagacac aaaagctttg gcttaaat 828 <210> 49 <211> 1735 <212> DNA <213> Oryzias sp. <400> 49 aagcagtggt atcaacgcag agtggccatt acggccgggg agagcaactt cgagtagcaa 60 agtagcaact tcaactccct ctcaaggaac tttctcaaag gcagaaaaaa ggaattttac 120 ccacttccga gtggagtttt actggaatct ttcttcttgt tttttcgggg aaatatgatg 180 ttcaccgcct tcaacacaga gtgcgactct tcctcccgct gcagcaccgc ttctccgtcc 240 ggagacaacc tgggatacta cccgtcacct gcgggatctt actccagcat gggatctcct 300 cagtctcagg atttcactga cctgacagca tcaagtgcct ccttcatccc caccgtcaca 360 gccatttcaa caagtccaga cctgcagtgg atggtccagc ctgtggtctc ctcagtggct 420 ccctcccaca gagtccaccc ctacagctcc agccccgctt accccagatc atccatgagc 480 tctccgtcca agatttgctc ttccagacgt ggcaaagtag aaaagataag ccctgaggag 540 gaggagaaga gaagaatccg cagagagaga aataagcagg cagcagctaa atgccgcaac 600 aggaggcgag agctcacaga cacccttcaa gctgaaactg accagcttga agatgagaag 660 tccagcctgc agaacgatat tgctaatctg ctgaaggaga aagaacggct ggagttcatc 720 ctggctgccc accagcccat ctgcaaaatc ccatcagagc tggagagcga gttccccctg 780 acctccatct cctccaccct tccatgcctc tccaccacag tgtcttccac gaccaccttc 840 acctccagcc agcaaaccat acctccaact tccaactcta tcttctccag ctccatcctc 900 tccaccacca cctcaacaac caccaccact gcctctgaca gcacagtcaa gatgagcgac 960 ctcgaggctt ccattctgga ggagtctttg gacctgctgg aaaaaacgga catgaagaca 1020 gcgcagtctg tgccagaggt tgacctttcc agctctcttt acagtaatca agaatgggag 1080 cctcttcatg cctcttcagc caacgtcgac tttgagcccc tgtgcacacc agtggtgact 1140 tgcactccgg cctgcacaac caacacgttc tctctggtgt tcaacttccc agaagctgag 1200 accttcccca cctgtggcgt tgcacacagg agagggagca acagcaatga ccattcctca 1260 gactcgctaa actcacccac cctgctcgct ctttaaggac attttctatt aaaaaaaaaa 1320 tccttcctat caagtcttga aaaattagca gaggcatgag ctttggaaca gatggcaaat 1380 cctatgttac ttgcctttat ctctacttgc ctattaacac caatgtagca agttattgag 1440 catgatttaa acaaaaaaaa cacattgttg ttactattat atttatggtg ctatagatga 1500 caatacttgt tgtgttttca gagcaatagt tatatttttg gtttgttttc tggttatttg 1560 aatgtcaaag taaatttttt tctcccatta aactgctgtg gtgccatggt ctgcaagtcc 1620 atcagactgt gaagttttct gtgaaaacat tagtgcttgt aatttattaa atttctattt 1680 atttattttt tacagacgaa tgttgtgtaa ataaaacata tatatgaaat gaacg 1735 <210> 50 <211> 1210 <212> DNA <213> Oryzias sp. <400> 50 tcaacgcaga gtggcattac ggccggggct ttccaaatgt tttgttccaa agttcactga 60 acctgtaaca tttaaaagac actgttcatg gggacatgaa acattacacc caaaggttat 120 cacaccttct cctcaccatg aaaaccttga ctctggcttt ggtgttttgt gcagtgttat 180 cactggctaa tgctcactgc ttctttatgc gtgtcgatcc tgacgcaact cattgtcagg 240 atgagtcgga tgggagctgg catgcagtgg ggtcctcatg gataagtaac tgcatgaact 300 gcacctgctt ctcatgttgc tctacgtacg ccattccatg gattttcccc gaagaggact 360 gtgaatctgt ctttgacaat tcaacgtgtg aatacattgt gcgaagaaaa gatgatccat 420 ctgtgctgtg tccagtcacg ggtgcagtag ggaaatgaag ccattgttga catgttttac 480 cacaatgtca gatacatttt tataaattgt gctctaagca agatataatt acccaaatta 540 agttacattt attaatttgt atagtttaaa tgacatattc acatgcctac tgcaatatga 600 ctattataaa tacaaataaa atctaatgtt tgttgtatct acttcaactg tttagaagtg 660 tgaagtacca attacattta taaccagaga aaagtaagat cctgcatgtg aagatttttg 720 aaaaacaaag taaaaaaaaa tagtttttga ttaaataaag acttgatgcc cacgaatata 780 atagtcgttc cacagaaata ctttggaaac acttgtatac acaacttttt gtataatttt 840 atgttttaga acagaaaagt attttttgga ttgatacatt taaaaaagaa agttaaaagt 900 aaagtgaaga aatatgttaa catctgtgta gtttgctctt ctcaaaatta actgttttat 960 aattgacaca cgacaaaatc attatgtcaa attgaatgat ttgcaagtca aatactgagc 1020 aaatattaaa caaaaattgt cacttcatga catttcagaa agaaattata ttagtgatgg 1080 atgagatcta aactacaaca atatcactga caaagtattt ttctaagaat ttcagaccat 1140 ggctgctgtg aaaatacttt ccaacaagat tattttccag gttttcaaaa aaaatgtcat 1200 tctgaatctc 1210 <210> 51 <211> 692 <212> DNA <213> Oryzias sp. <400> 51 gcagtggtat caacgcagag tggccattac ggccgggggc agtctctgcc tgttcaacaa 60 ggaacagtca accagaagac tgtgttccag aaactgccga agattgttac aaacctgcgg 120 acatcatgcc cagaaacctg ctgatgaacc cttgtggtga agatgaccta aaattctggg 180 agctggtgga caatgaaggg gacggatgga aagtggagga catgcagggg gacgacggtc 240 aagacatccg taatggggaa gtgaaaaagt attttgcgac ttcttttgat ttgtgttcaa 300 agatgcaagt gatcgatctg ttgactgaag attacacctc agagcaactg gacgctcagc 360 cagaggtcac ggtggaggac tggttctgca gtcgaagaga ctgcggctgc gagtaccagt 420 tggccgtgag cttgctagat aaaacaacgt gatccttcag gagttcctac cagagcgggt 480 ggtcatagac cctgagaagg acgacggctc ctggaagcag accaaacata cattttctgg 540 atacggccct ggcttgcgct tcatctcctt tgaacatgga gggaaagaca ccaagtactg 600 gaagggttgg tatggagttc gggtgactgg gagctccgtt accatcaacc tgtgaactgg 660 tttaatccgg aaatagagaa aagaaaaaca gt 692 <210> 52 <211> 692 <212> DNA <213> Oryzias sp. <400> 52 gcagtggtat caacgcagag tggccattac ggccgggggc agtctctgcc tgttcaacaa 60 ggaacagtca accagaagac tgtgttccag aaactgccga agattgttac aaacctgcgg 120 acatcatgcc cagaaacctg ctgatgaacc cttgtggtga agatgaccta aaattctggg 180 agctggtgga caatgaaggg gacggatgga aagtggagga catgcagggg gacgacggtc 240 aagacatccg taatggggaa gtgaaaaagt attttgcgac ttcttttgat ttgtgttcaa 300 agatgcaagt gatcgatctg ttgactgaag attacacctc agagcaactg gacgctcagc 360 cagaggtcac ggtggaggac tggttctgca gtcgaagaga ctgcggctgc gagtaccagt 420 tggccgtgag cttgctagat aaaacaacgt gatccttcag gagttcctac cagagcgggt 480 ggtcatagac cctgagaagg acgacggctc ctggaagcag accaaacata cattttctgg 540 atacggccct ggcttgcgct tcatctcctt tgaacatgga gggaaagaca ccaagtactg 600 gaagggttgg tatggagttc gggtgactgg gagctccgtt accatcaacc tgtgaactgg 660 tttaatccgg aaatagagaa aagaaaaaca gt 692 <210> 53 <211> 415 <212> DNA <213> Oryzias sp. <400> 53 aagcagtggt atcaacgcag agtggccatt acggccgggg agtctgttct gaatatcaca 60 ggattacaaa atgagagctg cagtgacctt cattctcctc ctgatagctt tacatcaagg 120 tgaggctctg gaatgtaact tctgcttctc cacgggaggg gaactgtgca ctcccaccag 180 cactcagatc tgctccagaa cccagaatgc ctgtggtgcc gtcattttcc aaggagctct 240 caaccgttcg tttcgtcagt gcatgaacat ggcggtgtgt cagggctaca tctccactcc 300 tggaattctg gccacatgct gcagcactga cctctgtaac tgagactgtc gtcagagcct 360 gaagacactt ctgaccttcc agcttcacgc tagtttgatt cctctgaaga aattc 415 <210> 54 <211> 1585 <212> DNA <213> Oryzias sp. <400> 54 aagcagtggt atcaacgcag agtggccatt acggccgggg ctactcggac tgaagaacta 60 tcatccgtaa gagacctcca ctccacctaa gcagcagcag tcatggcaga cctccaagtt 120 ggaatcaatg gctttggccg cattggccgc ctggtgctga gggcctgcct ccagaaaggc 180 atcaaagttg tggccatcaa cgatcccttc attgacctgc agtacatggt ctatatgttc 240 aaatatgatt cactcatgga cgttaccacg gagatgtctc ccacgaggat ggcaagctga 300 tcgtcgatgg aaacgccatc tctgtcttcc agtgcatgaa ccagctgaga tcccctgggg 360 tagcgctgga gccaaatacg tagtcgagtc cactggagtg tttctcagtg tggaaaaggc 420 ctcacttcac atccagggtg gggcacagcg cgtggtcgtg tctgccccct cacccgacgc 480 tccaatgttt gtcatgggag tcaacgaaga caaatacgac ccctccagca tgaccatcgt 540 cagcaatgcc tcctgtacca ctaactgcct ggcccccctg gctaaagtca ttcatgataa 600 ctttggcatt gaggaggccc tcatgactac agtccatgcg tacacagcca ctcagaagac 660 agtggacggt cccagcgcca aggcctggcg tgatggacgt ggtgcccacg ctaacataat 720 tccagcttcc actggtgctg ccaaagcagt gggcaaagtc atccctgctc tcaacggaaa 780 gctgacaggt atggcgttca gggtgccggt ggctgatgtg tctgtggtag atctaacatg 840 ccgcctgtcc aagcccgcat cttatggtga gatcaaggag gccgttaaga aggctgcaca 900 tggacccatg aagggtgtgc tgggatacac tgaagaccag gtggtgtcct ctgatttcat 960 tggtgacact cactcttcca tctttgatgc tggtgcaggc atctctctca atgacaactt 1020 tgtcaagctc atttcctggt atgacaatga gtatggctac agcaaccgcg tggctgacct 1080 gctgctgtac atgcactcca aggagtagac acttcctgcc cagagaggaa gttaggaaag 1140 ggaccacccc aaccctcctc attaccgccc tgctctttta cacaccagcc cacccatagc 1200 caagtcatag cgtccccgcc cttagctcta ctacagtata tgtaggcggc agcaacagtg 1260 tttgaatgtt ttgagttcat ctgtctcttc ctttttcatt acgaggcgac cggtcggtaa 1320 cagtgttttg tgtccgtgtg cccctttaaa gctccttctg cccttctaga tgttgttatg 1380 actgagttct gtggtgacta ctgtatccat ctcactgtgg ttgtgtttta aaagcttatt 1440 caccagtgga aggaggaagt tgaaaggcag gccttgtgtt atcctgcttt aaagtttaac 1500 gagaccaaga aataaagttt gaaaaaacct caaaaaaaaa aaaaaaaaaa ggccgcctcg 1560 gccactctgc gttgatacca ctgct 1585 <210> 55 <211> 466 <212> DNA <213> Oryzias sp. <400> 55 aagcagtggt atcaacgcag agtcagccga ggtcaacaga ctgacaaggt tggcgatcaa 60 tcggccttct cggtcatcag gaacatcacc acctatcctc ccttcgctca ggttgttaaa 120 gactggatcg atgtggtcaa catcccaggt cattttaaaa ccgacacagg cgagttcatc 180 tgtgacatat ctggagttta ttacttcaac tttcactccg ttgccaaggt cagcatgtgc 240 ctggccatcg tcagcaccgt tctggacaac aagctggggt tctgtgatta cagcaaaggt 300 ggtgaacagg tgctctctgg gggcgtggtc ttacagctga cagcaggtga caaggtgtgg 360 ctcgagtcct ttagagacca gcaagaggcc aaagtcatgg ataatagtga gagcaaacag 420 atcatcttca acggcttcct gatcttcaca gcccaatagc tcagat 466 <210> 56 <211> 1563 <212> DNA <213> Oryzias sp. <400> 56 ccttcagagg ggagctgaga gaaaaggctc tggagatggg agccaagtac cgacccgact 60 ggacgcctga ctcaacacat ctcatctgcg ccttcgccaa caccccaaag tacagccagg 120 tgaagtcggc gggggggctc atcgtacgga aggagtgggt gttggattgc cacaagagga 180 agcagaagat ctcctataaa cgttatttga tggatggaga cgagtcgagc tccgaaagcg 240 aaatggaggt tgacgaagat agcgaggaag aagtcaagac aaagactccg cagaagcagg 300 aaagacaagt cactccgaag aaaactccag caaagaggga tgaagaggag gagtacgctg 360 gatccactga tgtggaggaa ccaggagctg aggacgatga atcaggtgtg gacacagagg 420 atgagctgca gagggtggag aacgagagca ggaggaagaa agcagcggca gaaacgacag 480 tgaacgaaga ggatccttac ggggggtcga cagacgaaaa caccgacatt gaagctgagg 540 aggatcatcc catccccgag ctaccagatt tcctgagtgg aaagcgattt ttcctgtacg 600 gaaaattccc caacgatgag cggcgactgc tgttgcggta catcgttgcc tttaatgggg 660 tgattgagga ctacatgaca gagaaagtgc agtttgtggt gaccagtgaa gggtggcacg 720 attcctttga agatgccctg atggaaaacg gcaatctgaa cttcgtgaag cctgcttgga 780 tttatgccat caacgagcga cagaaactgc tgccctacca gccttacacc gtcgtcccct 840 gagctgcacc cctccctgaa catcctcaca aactgaggtt tgcaaagcca cgtttcacct 900 caaaagggca ataaatcagc gtctcttctt tatgtctcta cactgacttc acacctttag 960 gttaatctct tctacagaaa aatgtgtttg tgtcccttaa ggattcatta aaaatagatt 1020 tatttttagc tcccccatta aaggctggtc agccgaagcc tgtccgagca ctagaataac 1080 atttttcttg cgccacgttt cactgggaca ccatggaaaa agtcaaatct ctgttttttg 1140 tgtgtgtgta cacgtctctg ctcttcctcg gctacctcag gctacacggg ggaatgagta 1200 atcggcctgt cctgactgcc actcgcggcg ataagactca gaatacagtt gaattaatga 1260 gactaaacgc agaagccaca ggaaaactgc tgctagtttt tctacacaac aataaaaaaa 1320 tagtttaaat gaagacaaaa gcaattgaat ttaagtagta gaggcttcat ttttttttct 1380 ttgtgtgtta gagtttgatg ggtttgtttt attaatattt gtgttataaa tctaccagag 1440 acagcaataa gaacatacat tgtctaattc atgtggaaac atttgaaact ttgcaaagca 1500 gtttttaact ctcaaacgtt tttttattat tattattatt attatattgt tgtacctaat 1560 taa 1563 <210> 57 <211> 478 <212> DNA <213> Oryzias sp. <400> 57 aagcagtggt atcaacgcag agtgctgtgt cctgctaccg aattgagccg gggacaatag 60 ctgggattgt ctgcgtggat gtgctgctga ccctcatcat cgtagctgtc acctaccgct 120 gtgccagtta tagacataag aaagtagaaa atgctaacaa agtgtatatg aacgtccggg 180 ccaactgcaa gaaaacataa aaatggactc cacagttttt ggctttttaa cttgtttttt 240 attttctttc aatagatata acaaacttgt ataccaggtt acttaaaaaa acttgctcat 300 aagacatttt taatatatgt ttgctcaaat taaccatatc tcttgaataa agaattttga 360 aaattaaaga agcccattat ataaactatt attactttca ttcaaaatgt ctttgtcttt 420 tgtctgaaat gtttgctttt ttgaggtcct tgcataaata aatattccat atctacct 478 <210> 58 <211> 1055 <212> DNA <213> Oryzias sp. <400> 58 aagcagtggt atcaacgcag aggccgagtg ggcggttcaa atcctgaaga ccagcgggaa 60 gcccgtggcc gcgactctgt gcatcggacc ggagggggac ctgaacggag tcagccccgg 120 agactgcgct gtcaagctgg tgaaggctgg tgcagatatc gtgggggtaa actgccaatt 180 tgacccggag agctgcgtga agaccgtgaa gatgatgaag gagggcgtgg agaaggcggg 240 gcttaaagcc cactacatga gccagccgct ggccatgcac acccccgact gcaatcgcca 300 gggcttcatc gacctgccgg agtttccgtt cgctttggag cccagagcgt tgaccagatg 360 ggacgtgcac aggtacgcgc gggaagccta caacgccggc atccgttaca tcggcggctg 420 ctgcgggttc gagccgtacc acatccgagc cgtggccgag gagctggcgc ccgagcgcgg 480 tttcctgccg cccgcttccg acaagcacgg cgtgtggggc gccggcctgg agatgcacac 540 caagccttgg gtcagggcca gggctcgccg tgaatactgg gagaatctga agcctgcatc 600 tggacgcccg ttctgccccg ccatgtccaa accggactgc tggggctact ccaaaggcca 660 ccccgacctg atgcagcaga aggaggccac cagcgaggaa cagctcaagg ccgtctttga 720 aaaagctgcc gaaaacaaat gagagccccc cacccccccg agggtcacaa tgaattcaga 780 tcaattcaga agttgacccc cccacctaag ccgaagagat atgaaacttc cctgagcctg 840 acccgcagga acaactagtt tttaaagctt tgtttaggaa catttctgtc tttttgtagt 900 gtttttagcc gatttgtact ttaaaagctg cttagattaa ctgctgaagt aaagtcctaa 960 ccaccgagtg atgttatgat gcaaaaataa agctctgcta agaaaaaaaa aaaaaaaaaa 1020 aaaggccgcc tcggccactc tgcgttgata ccact 1055 <210> 59 <211> 1196 <212> DNA <213> Oryzias sp. <400> 59 gagtcggcat cctgagctgg caccccacgg ctcacaacgt tctgatgagt gcaggctgtg 60 ataacgtggt catcctgtgg aacgtggccc gcggggaggc ggccgtgagg atcgacaccg 120 ttcacactga cctcatctac tgcgcctgct ggaaccggga cgggtcccag atcctcacct 180 cctgcaaaga caaggcgctg cgagtgctgg acccccgcaa gggcaccgtc ctctatgagc 240 gggaaaagcc tcacgagggt tccaggccca ttcgagccat tttcacgtct gacgggaaga 300 tcctgagcac cggtttcagc cggatgagcg agaggcaggt ggcgctgtgg gatccgaaaa 360 acttctcgga ggcgctgact ctgcaggagc tggacaccag cagcggggtt ctgctgccct 420 tctatgaccc agacaccaac gtggtctacc tgtgtggcaa gggagacagc agcatccgct 480 actttgaagt gacggacgag gctccttaca tccactacct gtccatttac agcagcaagg 540 agagtcagaa ggggatgggc tacatgccca agagggggct ggagatcaac aaatgtgaaa 600 tcgccaggtt ttacaaactg cacgagagga agtgtgagcc cattgtgatg accgtacctc 660 gcaagtcgga tctgttccag gaggatctgt accccgacac cctcgggccc gagccctcgg 720 ccgaggccga cgagtggttt gcaggaaaag acgctcctcc ggttttgatt tctctgaagg 780 acggctttgt ggcgacgacc aaagccaagg agttcaaagt tcacaagagt cttctgaaga 840 ccacgagcgc ttcagcggcg agtcagccgg acgccagcgg ggaggtgcag tccttgaaga 900 aggaggtgaa ggacctgaaa gctgcgatgg aggagctgac caagcgcgtg atggagctgg 960 agagcaaaga ttaaagagga aagacgagaa aacgaagcca aaaatctgga tgaaagagca 1020 ggagcacgac ctgaaactgt tgttcgcttt gggtggaagt gagacaaagg gatagattag 1080 gaaacatttc cagtttgaca attcatttgt ttgattctag tgaccaattt ttacaaaaaa 1140 aagaaaaatc ctttttttaa tatatgattt tatttgaaat aaagtctttc gtgttt 1196 <210> 60 <211> 1489 <212> DNA <213> Oryzias sp. <400> 60 aagcagtggt atcaacgcag agtggccatt acggccgggg gacaaacaga tagggcggtg 60 aagtggtcga aagcacacca gtggatggtt tgtaacttac acaaagggag gactttactg 120 tgactgtggg aggaagctga ccgcaaaaat gtcaaacaaa atgatcgagc ttgaggagaa 180 tgcgacccac acaggtccaa aaggagtcat caatgactgg aggaggttta aactggaaag 240 tatggaccag gaaaacttgc cttctgcaaa aaaagaactg ctgagacaaa tgtcttcccc 300 caataaatca aaagatgact ccagagcaaa cctaaaccgc aagatgagtg tccaagagta 360 tgagctgttg aaggaagagg atgaagggtg tctgaagaag tacagaaggc aatgcatgga 420 agaaatgcac aacaagctca gcttcgggcc caagtttgaa ggcgtacacg atctggacag 480 tggagaggcc ttcctagaag tcatagagaa ggagcatcac agcacggtgg tggttgtcca 540 catctataag attggtatca aaggttgtga gcagctcaac agctgccttg actgcctggc 600 aactgagtac cccaccgtaa agttctgcag gatcgacgct gttgcgtctg gagccgctga 660 gcgcttctcg gatgaatatc tacctactct gctggtgtac aaagcaggag agctactagg 720 gaatttcctc tcctgcacac agcacctaag tgaagaattc tttgccacag acgtggaagc 780 ctttctcaat agctatggtc tgctgccaga aaaagagctg cccaagctgg aggatgaaga 840 agaaaatgat gtagagtaaa tcaagtgtgg ccactgtcag tgaaaagaag cagtgtcaaa 900 gatccaagac tgggaagatt catacataaa atatgatgag tattctttat tgccttagca 960 aataggaaat ccatatcatc ttctctagaa ctctagttct ggattattct ctaaaggaga 1020 cacaacaatg gcagccttaa aacaccaaat atgggagctg ctaattgact gaaggataac 1080 aaaacaacta aaacagtttt attttttaat tttgtaattt tagataatgt tacatactgt 1140 gtctttgtaa aaaggattca tacttatctt tttttagtct tagtttatag atatttaata 1200 ttgaaaatga ttttaaaata gtttgatata ttgttcctaa attatcttga acgtatagta 1260 caattcccat gtttctatgg catcactgta atgttctgct agaacttaat acatgaagga 1320 ctgaaatgaa ccataaaaac gttgtttgat tgtatatgtt aaagtagtga agttgactgt 1380 tgtctctgga gaacatttat gagaaacaaa ttaatcaata aaacatgaca actgaggaac 1440 aaaaaaaaaa aaaggccgcc tcggccactc tgcgttgata ccactgctt 1489 <210> 61 <211> 839 <212> DNA <213> Oryzias sp. <400> 61 cattgcgtgt gtttctcgca ctccggcaac cgtctggcct gggtttccca tgattccacg 60 gtgtctgtcg cggagggagg caagactggc acggtgaaca gcctgagctc agaggcgctt 120 cctctcctgt gtgcgacttt catcactgag aacagcatag tagcagctgg tcacgactgc 180 tacccggtgc tgtttgtgta cgatggcgcc aaaggcagtc tgacatttgg cggtaagctg 240 gatgttccaa agcaagctgc ccagaaggga atcagcgcca gagagcgttt ccagaacctg 300 gatcgccgag cctcagagac tcagggcact gacaaggacc tcaacacgct ccacaagaac 360 agcatcagcc aaatctcttt gctggaggga ggaagaaacc agtgcaccaa gttctgcacc 420 accggcatgg atggaggaat gggcatctgg gatgtcaaga ctctggagtc ggctatgaag 480 gacttgaaga ttgtgtgagc tgctctgcat ttacctgatc aaaatcaaaa tgcttctgta 540 ttaagaaaac tgctgcattg ttatacaaag cttcatggct gtatttttat caaaaatgtg 600 ccactctttt aaatttacaa acgaaacagt gttttttcgt ctacacatga caaggctctg 660 taaaaagggg ctggcctcag gttggcatat ggtacttatt ctgctgcatt agtacagtca 720 cagctcagtc tgagctcaaa gcttaaagca gccattttat atacatagca atggaaatct 780 ggaatctttt tacaaagctt atgctggtca taatatgcat ttaataaata atttctgtc 839 <210> 62 <211> 703 <212> DNA <213> Oryzias sp. <400> 62 gcagtggtat caacgcagag tggccattac ggccggggac acgctgccga gctcgccgtc 60 gactgaagga gcccgccacc cgagctgagc cggaaccgtc atggccgtgt ggggcaggaa 120 ggcgctcctg ggggtcgccc ctctgtggat gtgcttcggg agagccgagg cgctggctct 180 gtccgagcct cagatctgct acgtgctgga cggcatcctg ttcctgtacg gcatcatcct 240 gaccgccctg tactgcagaa tcaagatcaa cagcgccaga gaggccaacg gagctaaagg 300 cagacagaac gtggaggagg gcatctacac gggcctgaaa cctcacgcca ccgacacata 360 cgagaccatc ggcatgaaga agtgatgcgg caggaaggaa gtccacttct ttttttctct 420 gtatttaata accgcattta ccgctctgct tttatagagc cttttcctca cacgctgttt 480 cagaatgtcc agcatgcatg caactttgaa atctgcttga caaacatgtg gatagaacca 540 gacccaatcg tttgattcat ttttgtagaa taaaaaaata aagctttgaa gcacaaactc 600 atgaataaaa gaacgcaggc agaaagattt taatttattt atcactcaaa ggagaaaaat 660 cattcagcaa attaaaatga agataaagtt agaaacctta aaa 703 <210> 63 <211> 733 <212> DNA <213> Oryzias sp. <400> 63 atactttgac gacagcaagc agtttaagcc ggagcgctgg ctgagggaga acagcaacat 60 caaccctttt gctcacgtcc cctttggaat cgggaagcgg atgtgcatcg gccggagact 120 ggcagagctg cagctgcagc tggcgatgtg caggttggtt agagactatg aaattgtggc 180 aacagataat gaaccacttg atgtgataca ctctggactt ctggttccca gcagagaact 240 gccagttgcc ttcatcaagc gataaagctt catttgaaag tcttctggat gtgacgacag 300 accgtcgatg tgtctgttgt gatgatcctg acggtccagt gactcctacc actgctgctc 360 tgtacataga aaaatatcat aattgcgcct gaaatacctc attattattt aaaagtagtt 420 ttgcaactaa gtacgactga gcaaagcacc aaatgtaatg tacgatttaa aggttttaac 480 gaggcagctg tctttcaatc ttcattatca gacgtgtaga atgtagatgt taaactatta 540 ctttatacta ttattgtttt gttgtgtttg tacatatcca tatatctata tatgtatata 600 tcagtctgtt tatctttttt agtgtttatt ggtagaatcg ctactataat taatatctta 660 ttcctgtacg ttgttgtatg tagaaacata ttttattctc tcaaataaat gcatcgatga 720 atgcatcaaa aac 733 <210> 64 <211> 443 <212> DNA <213> Oryzias sp. <400> 64 catcaacagc cagtatgtgt ggacttttgg tggaggaacc agactggatg ttggaagtga 60 taccagtcca tccctgaccg tgctcctccc ctccacgaag gagctgcagc aggggaaggc 120 cacactcatg tgtgtggcca acaagggctt cccctcagac tggactctgt cctggaaggt 180 ggtcggcagc agcaactggg aggagatcag gagccccggg gtgctgcaga aggacggcct 240 ctacagctgg agcagcaccc tgaggctctc tgcagaccag tgggagaagg tgacctgtga 300 ggccaagaga ggatcccaga ctgcattctc agagactctc aggagagacc agtgctccca 360 gtcctgaact caaacattct ggatataaat ctgttttcta tgctgttctt cttttacatt 420 ctcttctttt caaactctgt ttg 443 <210> 65 <211> 758 <212> DNA <213> Oryzias sp. <400> 65 aagcagtggt atcaacgcag agtggcatta cggccggggg gactgcgagg agtttggagg 60 agtttggagg agtttggagg agccgcgggt ggaaatgtct cagtactcag gaaagatcgt 120 cctctacgag gggaaaagct tcacggggag gaagctggag ctctgcagcg aatgcgacaa 180 cttccaggac cgcggcttca tgaaccgggt caactcggcc cgcgtggaga gcggcgcctt 240 cgtctgcttc caccacccgg acttcaaggg tcagcagtac atcctggagc acggggagta 300 ccccgaattc cagcgctgga acggccacaa tgatcacatg ggctcctgca gaccaatcag 360 gatgcacgga cagcactacc ggctggagct gtatgagggg gaccggttca gcggtccgtg 420 tgtggagctg agcgacgact gtccgtttct gcaggccaga ggtctgacca aaacctgcat 480 caactccctc agggtttatg gagatggagc gtgggtgttg tacgaggagc caaactaccg 540 cggtcggatg tacgtcgtcg agcggggaga cttcggctcg cccgcggact ggcaggcgga 600 gaaccccaac atccagtctg tgcgcagggt ggctaactac ttctgaagct agctcccatc 660 cgcctcatgc agctgacaat aaagatgcag caaacgaaca aaaaaaaana aaaaaaaaaa 720 aaggccgcct cggccactct gcgttgatac cactgctt 758 <210> 66 <211> 818 <212> DNA <213> Oryzias sp. <400> 66 ctgacggcgg accagctcgt gctgcttttg gaacttctgt tggaggaacc ggagctgagt 60 gcggcatcca tgcacgccct gtacaagatc tacaatctgc acagacaaga cgctgaggtt 120 cgacatcgct ggtgtgagct ggtggtcaaa catgctttta ctcagggcta cgcagatgtt 180 gaacattttt tactttacga tcaggccatg ggtgtgtatc tgtacgggga actgatgctc 240 caagaggacc ctgaacagca ggctctggct cgccgctgcc tctcagcagt tgcagaggaa 300 atggaccagt cagcgcgcag agtggtggag gagatgatcc tgtgactctg gaggttgcag 360 agacgttatc aggatccaga actcacctgt ttttctggga tactgggcac caaccgtcca 420 aactgttcac acaagactat tacatcttta cactgtaaat gttttaggaa ttaatttttt 480 tttttactca acgcagagag ccatgtttta gagtctgagc tgctgtgtta gtgtgagttc 540 atgtctgttt agtctaaagt catcatctgg tttttaagag tttagactga aaatacattc 600 agtgagttct tatttatttt attttttttt taaaaacaaa ctgcatctct gacccacgac 660 cgatctgaaa tggatgaatc agcactttcc aatgcgttta aataaaagtt tatttatgtt 720 gtgtaaatga tcaataatcc attcaataaa aacaaatcct acaaaaaaaa aaaaaaaaaa 780 aaggccgcct cggccactct gcgttgatac cactgctt 818 <210> 67 <211> 230 <212> DNA <213> Oryzias sp. <400> 67 taagctggtc accacttctg ctttcatgtg caatgctccc atacacaaag tgtttgatgg 60 aatcggcgag atggtgaaag aaactctgaa gctggcttag ctccagcttt ttatgttatg 120 ttttatagat ttgctgacaa attcttttct ttctatgttt ctgagtggat ctgaaccaga 180 cgtcacactt tttttaaact ttcaataaac tcttgttttg tttgaaaaat 230 <210> 68 <211> 880 <212> DNA <213> Oryzias sp. <400> 68 tcagtctggt tctagatcaa acagatcaac atcactggct ttaatggaat taacagaaga 60 aataactatc actgaagtga ataaatatgc aacaggagtt ttcatagatc taaaaaaaaa 120 agcttttgat acaataaatc atgatattct aatttataaa atgaataaac ttggagtgcg 180 aggatcagct ttagactggg agaaaagtat ttacacgaga ggcaacaatc tgtgacgatg 240 tgagaccata actcaacagt tttagacatt gtgtgtggtg tccctcaagg ctcagtgctt 300 gggccagttc tatttaacat ttacatcaac gatatagtta aaacatcaga tgaaatgaaa 360 ttcatgttat ttacagatga tacaaacatc ctgttttttg acagaaatat ccaggaactc 420 ctctagagtg accagaggac tgtataatct gaaggactgg tttgatagga acaagctgtc 480 acttcattta gacaaaatta aaatcatgtt ttttggtaaa aaaaaataca aaagagcaaa 540 ttaaaataag aatagcagga gtaacattag aaacagtgaa taatatcaga ttcctgggcg 600 tgacaataga tgacgagatt tcctggaaac agcatattag ttctatacag acaaaaatat 660 ccaaaagtat ttcagtcatg gacaaagtga aacatatttg accttcagat cacgtcagat 720 attatagaat tctctggtgt tgccgtactc aacctactgt tctgaaattt ggggaagtta 780 aaacaaaact tctttaaatc cactataaat gttacaaata gagcagtaag aatcattcat 840 aaaatgtaag ttatcgccat cacactaatc aattatttct 880 <210> 69 <211> 2119 <212> DNA <213> Oryzias sp. <400> 69 aagcagtggt atcaacgcag agtggccatt acggccgggg actgagctga aatctttcag 60 aggaacagca cgtctctctc actctgcaga gaagggaaga ttttatgttt ctgtttattt 120 atttattttt tactttaaaa aaaaaaaatg ggagattatg atgaggacac tgcatttctt 180 ggacagaccg gtccttattt ttgggtcacc ttctttcttc tgaacacggt ttttgtgtcc 240 actggattca acggacttta cataatcttt gctggagcga ccccgaagca ccactgtcta 300 attccagatg ttaacttgac ggaagagtgg aggaacgcca tcattccagt cacagaagtg 360 gatggagaag aaattagaag tcagtgcagc aggtataagc tcgacgtagt taaaaacctc 420 tccagtgagg ggtacattcc aggaagggat gtcaacctca ccaacctgca gcaggaagcc 480 tgcttagatg gttggagcta cagcaaagaa atctaccaat ccaccatcgt cactgagtgg 540 aaccttgtgt gtgacaacca gtggaaagtt ccatttgcat cctcatctct ctttgtggga 600 tacctttttg gctccctaat atcaggacaa ctttctgaca ggtttggaag gaaaaaagtc 660 gttttcctgt ccctggcggc ccagtgcctc tccgttctcc ttcagtcttt ttcccaatca 720 tggagaatgt tctgcatcat attcctcttt gtcggagcct ctcagatttc catctatatc 780 tctgcttttg tactaggtac ggaggtgttg agcaaaacca tgcgggttct cttcacaaca 840 cttggggcat tcttgtttta ttgcatcggc tacatgactc tgccctggat tgcatttggt 900 atcagagact ggaggaccct gcttgctgtt ctgtctgcaa cttcggtcgt ttacatccca 960 ctgtggtggt tcgtctcaga gtctcctcgg tggttgatta ctcagggacg agtggttgag 1020 gccgaggcca ttgtgagaga tgctgccagg agaaataaag ttgctgctcc tcctgtgatc 1080 tttaaagagt ctgagataca ggaaatatct gctggcagga aatacaccat gcttgatatt 1140 ttgaaattta agaacatccg gtgcatcaca gtgatgtgtc tccttttgtg gatgtcaata 1200 aacattgggt actttggatt atccctgaac acctccaacc ttagtggcaa tccctttatg 1260 aactgcttcc tgtcagccat cagtgaagtt cctgcctaca tagtttccac tttgctgctt 1320 agggtttgcc caagaagaat tcttctgtca gtgttcctct tcctgggtgg aggagttctc 1380 cttctcatcc agttcatccc tgagactgtt cagcaggttg ctttggctct ggaaatgact 1440 gggaagtttg gctttaccat ggccttcagc atcgtgtaca tctacaccgc tgagctctac 1500 cccacagtgc tcaggaacgt cggcatggga gtgtgctcct ctgctgctcg cataggcagc 1560 atcacagcgc cctacatcat ctatttaggt acatacgata aagttctgcc ttatatttta 1620 atggggagtc tcaccatctc ctcctcgata gttaacttgt tcctccctga gactctccac 1680 agagatctac cagaaacagt ggagcaaatg caagaatgtc aggggccgtg caaaagagcc 1740 acaaagagga aatgtattca aaatggagcc acacacaacg cactctctca atgtgaagcc 1800 aaatctgaag gggaaactct caatctgtag ttactttatg tggcaggact taaagtctct 1860 tttttgttag aagtggtcaa acattaaaag aaaactccct tgtgattgtt atatttacat 1920 agttacataa acctaaccag ctgttttgta ggacaggagt gaaaacagtt tctctttttt 1980 ttggtaatta aatcaatgta tttgtttgtg attttaaggc aatatttcta cagctaataa 2040 ttaactgata ataattataa ataataattt caactttttt aaattgaaaa gttaaaaagg 2100 tcatttacta tttttattg 2119 <210> 70 <211> 615 <212> DNA <213> Oryzias sp. <400> 70 nacygucsam nmraskryas atstacacct tcagccactt tcctgacgac aagagtggcg 60 agtggtttgg ttatctgacc cgcgatggca gcgtggctct ggacttcatg ggaggcccct 120 tcaaagggtt cttccatgtg ccccgctgcc tgctgatgtg tgaacgcctg ctggacgatc 180 tgctgcaaac acacgacgaa tgaattctac aactgaaata aaaagaaatc aaagtgtctc 240 tctgttcaga agcactgaag ctcttcttct cacttttctg tctcatttct tctgcttttg 300 tgttgatttc tcattgattc cagcggagga gcagcgttcg acagtaagga aagactgttt 360 tcatgaagga gtttgggacg gacaacacat gctctgagct ccacctcact tgctggggcc 420 ctctcctgtc atatatgggt ggggtctgcc tgtaggatgg agtctggggg tcgggggccc 480 atctttccac cttaaatgac tagtctaaag taatttaact taaaatctaa tttaaaaaat 540 aatagaaatt acaccatatc aagttataga atttatatat tgcataaaca tattattaat 600 attaattgcc ttgcc 615 <210> 71 <211> 1067 <212> DNA <213> Oryzias sp. <400> 71 aagcagtggt atcaacgcag agtggccgag gcggcctttt tttttttact atgttttatt 60 ctgttcatgc tttggattca catgagaaaa atacgagggg atcctgaaga gaaacaggag 120 aactaggaaa gcaacctttc gttcatgctg tctcaaagaa attaaaaatt tcctgatgta 180 gagcttctga atgggatcgg ggtgctggag gggaactggg cgcttcagtt ggcagcgatg 240 gtctggtcca cccaggcacg cagcttagtg actcgggcgt acacaccagg catggaggtg 300 ctgcaggttg agcttcccca ggacacaata cccaccagag tccaggctcc gtccttctgg 360 cagaccaagg gaccgccgga gtcccccatg catgaagagg ctccggaggc accagcgcag 420 atcatcagat ctgtgatgtt gctgccccag tagctgctgc agttagcatt ggtcaggagg 480 ggcagggagg cctgctgcag cagagcgggg gtgttggcat tggagtggtg ggtcagaccc 540 cagccagagg tcatacacat catgcctcca gggaagttgt cgttggtttc agccacacac 600 acgggggaga cgcgggtgtt gagctgggcg ggggtggcca gcttgatgag cgtgatgtcg 660 ttgttgatgg tgtaggagtt gtactgaggg tgcttgaaca cccggccaac attcaacacc 720 tggatgttct cagcgttgga gttgcggtcg tgttctccaa ggatgacgta gtgagaagtc 780 ctgacgttgc agtgggctgc ggtcaccacc cagttctcat tgatgagaga gccgccacag 840 aagtggaatc cagaccaatc ctgcagggac acctgccagg gccaggagtg aggcaccgcc 900 tcctcgccat tcacaatccg agagtaaccg gtcacgatgg gggagatggc aggcctccca 960 caaccatggg cagcaccaat gaaggcgagg caagagagga tccacaggaa ggccatggct 1020 cgatcatccc cggccgtaat ggccactctg cgttgatacc actgctt 1067 <210> 72 <211> 1099 <212> DNA <213> Oryzias sp. <400> 72 gtgtggttca catcattaat gctgagatgg aaaattatga acctggggtc tacactgaac 60 gcgtgttgga gtccatcaag ctcctgtctg aaactgtcat gccccgattt gctgaacaag 120 tggaggtagc aatcgaagcg ctgagtacga accccccgca ggcctttgag gagaacgagt 180 tcattgatgc ctcccgtttg gtgtatgatg gcgtccgtga catccggaaa gccgtcctca 240 tgataaggac accagaagag ttggaggatg actcagactt tgaacaagag gactatgata 300 ctcgcagcag gaccagcgtc cagaccgaag atgaccagct tattgcagga cagagtgctc 360 gggccatcat ggctcagctt ccacaggagg agaaggccaa aatcgccgag caggtggaga 420 gcttcagaca ggagaagtgc aagctggatg cggaggtcgc caagtgggac gacaacggca 480 acgacatcat tgtgctagcc aagcagatgt gcatgatcat gatggagatg acagacttca 540 ccagaggcaa aggccccctg aagaactcct ctgatgtgat caacgccgcg aagaagatcg 600 cagaggctgg atccaggatg gacaaattgg ctcgggctgt cgccgaccag tgccctgatt 660 cagcctgcaa gcaggatctg ctggcctacc tgcagagaat cgccctgtac tgccaccagc 720 tcaacatctg cagcaaagtg aaggctgagg tgcagaatct gggtggagag ctcatcgtat 780 ctgggctgga cagcgccacg tctctaatcc aagcagccaa gaacctgatg aacgccgtgg 840 tgctgaccgt caaagcctcc tacgtagctt ccaccaagta ccagaaggtg tacgggacgg 900 ctgcggtcaa ctcgcccgtg gtatcttggc gcatgaaggc gcccgagaag aagcctctgg 960 taaagcggga gaagccggag gagtgtcaaa cgcgtgtacg gcgaggctcc cagaagaaac 1020 acatctcccc cgtccaggct ctcagcgaat tcaaggccat ggactccttc taagaggttc 1080 aaggaaaaag ctgcgaaaa 1099 <210> 73 <211> 592 <212> DNA <213> Oryzias sp. <400> 73 tacaccttca gccactttcc tgacgacaag agtggcgagt ggtttggtta tctgacccgc 60 gatggcagcg tggctctgga cttcatggga ggccccttca aagggttctt ccatgtgccc 120 cgctgcctgc tgatgtgtga acgcctgctg gacgatctgc tgcaaacaca cgacgaatga 180 attctacaac tgaaataaaa agaaatcaaa gtgtctctct gttcagaagc actgaagctc 240 ttcttctcac ttttctgtct catttcttct gcttttgtgt tgatttctca ttgattccag 300 cggaggagca gcgttcgaca gtaaggaaag actgttttca tgaaggagtt tgggacggac 360 aacacatgct ctgagctcca cctcacttgc tggggccctc tcctgtcata tatgggtggg 420 gtctgcctgt aggatggagt ctgggggtcg ggggcccatc tttccacctt aaatgactag 480 tctaaagtaa tttaacttaa aatctaattt aaaaaataat agaaattaca ccatatcaag 540 ttatagaatt tatatattgc ataaacatat tattaatatt aattgccttg cc 592 <210> 74 <211> 438 <212> DNA <213> Oryzias sp. <400> 74 tgtcttgatg gaaaaattgc ctgacaacgt tggaaaaaaa agatttctta ccccgtgctc 60 atcaacattt gtggcatttt gaacaacctg gtcatcggcg gccttgaagc tgcaaaaatg 120 atcaccgaaa aggatggctt gcccaaacta ataggaatca gggattctgg agacattacc 180 gccgaaggtt gtcaagcatc cagatcagca ggaacagtcg tatccaacat gtactattat 240 aggaaactgc acaaactgta caaatcgaaa ggctacaaaa gtggagactt tgctgacaag 300 tttgcactct agacctttct cctttaagca aagttcattc tttatcttca agattttgca 360 ccaagaccac aagctaagca aagaaaatga ctgtgtttta ctacaacaaa tatctttgga 420 ggcggtacct gtggatcc 438 <210> 75 <211> 1282 <212> DNA <213> Oryzias sp. <400> 75 aagcagtggt atcaacgcag agtaaccatt acggccgggc agggatgaag tgaatgtgga 60 ggtggactca gccactgggc cagaactggg caccgttctt tcagagctgc gctcccagta 120 tgaagggatt gtccggaaga acaaggcgca ggccgagcag tagtaccgca agaagctgga 180 gacagtgcag aatgaggtga aggagagcaa tgaagctcta agaggagctc agggagagct 240 gactgagagg cagcgcttcc tgcagaccct ggaagtggag ctggacagtc tgcacaaaca 300 gatagcagct ctggaaggaa acctggccga gacgggccag aaatattctg gtgaaatgga 360 gcgcttgcag gtcacgctga gccagctgga agatgacctc tcccagctca ggctggacat 420 gcagcgcacc aagaccgact atgaacagct tctccgcatc aagcagaacc tggaaatgga 480 gatcgctact tacaggcgct tgctggaggg agaagaagca gccaaagaaa ttcctcctcc 540 accaaaaaaa gaaccggacg tccgcaccag gaagattgtg aaagtggtga ctcaaactat 600 ggtgaacggc aaagtggtgg atgagtctag tgaggtggag caaattgaag agtcaaagaa 660 atagaaatgg agacaccgat cgaagctgaa aaagatgttg tttttctttt tatgaagcag 720 ttaaggaaat gtattttagg aatcataaag aaaaaatatt tacatactgt acatggagca 780 ttagccttct attctattgg gaaaatatta ttcaattgag tcagttaatg tgctataagt 840 tgctgttgag tataagatgt agacacaaac aaaattacac acaagtcaca cttttgggag 900 aaatttattt aacaaaaaac atgaacagct gaattctttg tctgtgctcc actgaggctg 960 gcgcttcttc ttctgtgttg caaaaacaga taaacaccta cagtgtcttc aagagaatat 1020 caatgtatgc atgagccttt caaaaaatta aataaaaaaa aataccaaac ataggccgca 1080 cccctgacca aacgattcaa aaatgtgaat tatgctccaa aattacagtt gtttttcatt 1140 attcttgatg ggtctttagt agttgtgtat gtgtgtgtct tggtttgatg taggtttcta 1200 ttagaagaga acagagtgaa ctaataaaaa actgcatact tagaacactt ggaaaactgg 1260 taattttgtc tttactaaat aa 1282 <210> 76 <211> 733 <212> DNA <213> Oryzias sp. <400> 76 aagcagtggt atcaacgcag agtggccatt acggccgggg actaaggatt gaagatggca 60 ggtacagact ccggtacggt ctcctatgag gaattgaagc atttattggc aaacaacccc 120 aatctatttc tggttgatgt acgcaaccaa gacgagtggg atgctggaca tattccaagg 180 gccattcaca tccaattgga cacggtagag gctgcctttt caatgaaccc agaagacttc 240 agagccaggt atggagtgga gaaaccgccg ctgaattcac caaacatggt gttttactgc 300 cggaccggaa aaagatccgc acagggtttg tgcacggtca aacgcctggg atatgtcaat 360 gttcgcaatt accctggcag ctacacagag tggtctgcga gagaaggaag aggctcatcg 420 taaactgcac acaagtcttg tatttttgtc ttgcttttgt ttttttttta aattggaaaa 480 tgtttttagg caaagccatt tgagtcacct tagttggaaa agtgctacaa aattaaaatt 540 tgatttgatt tgatttgaag aaagactgat aatgctaatt gttcccattg aggacacgaa 600 gtgtcacttg taagatgagc taccaaataa aatattttgc tctttgtgct tgttgtgtta 660 cttttatatg aatataaaaa gaagaggaca aaaagatact ttgtaccagt tcagttttgt 720 gacatacatc tgg 733 <210> 77 <211> 1941 <212> DNA <213> Oryzias sp. <400> 77 aagcagtggt atcaacgcag agtggccatt acggccgggg gacatgcaag ctgaagctct 60 cccacacctg acaggaggag ctcacacaca gtcatgagga tgaggaagct cctgtgggtg 120 agtctgctgg ctctgctgct gctgcaggcc tcctgcatcc acgccaacga ttcagacgac 180 agcacagagg agcacagaaa tgctcaagac gacacaacaa aggaaaccac agaggaggcc 240 tcggcagaga cagacaaacc tgaagagcca ccgaagaagg agaaaacaac agagatagaa 300 gaagagcaag acgtcatggt tctgcacatc aacaacttcg acagagctct aagcgagaat 360 gaacatctac tggttgaatt ctatgctccc tggtgtggct actgtagaag atttgagccc 420 atttatgccg atgctgctgg gatgttgaag gaggaaggct cagccatgcg cttggccaaa 480 gtggatgcca tcgaggagaa agagctggct gaagaattta acatcgacag cttccccact 540 gtgaagctgt tcatgaatgg agaccgaaag cagcctattg aatacactgg taagaggaca 600 ccttcaggaa tagttcagtg gttgaagcgt cgctctggac ctggagctgc agacctggac 660 tctgcagact cggcagctga gttcatcaac agccacaacg tcacagttgt tggattcttt 720 gatgacctgg aaagtgaagc tgccggtgtc tttaaggaag ttgcttttga tttgacagat 780 gttgagtttg cagttacaac atctcctgaa gtctttaagg agtatgaggt gacatccaac 840 tcagtggtgc tgttcaagaa gttcgatgat ggcagagcag actttgcact gtctgaagag 900 gacaagctcg acaaacacaa cctgacaact ttcattaagg aaaacagtct ggagctgatc 960 gtctcattca accaagagac atcagacaag atcttccact ctcgtgtgcg gctgcactgc 1020 cttttcttca ttaactccac tgtgcagagt cagatgagcc tgctggagga gtacaaggct 1080 gtcgccagag agtttaaagg aaaggtgctg tacatcatga tcgacatcat gacatctgtg 1140 tcacatgtgc tgaactactt tggcgtgtta gagacggatg taccgactgt acgcatcatc 1200 aacatggaga cacaaaaaaa gttcaaaatc gactctgacc tgaccattga ctctctgaga 1260 cagctttccc aggacgtagt tgatgacact gcacagccgt acttgcggtc tgaggagatc 1320 cctgaggact gggacagggg cccagttaaa gtcctggtgg ccaagaactt tgagtctgtt 1380 gctatggacc ccaccaaaag cgtctttgtt gaattttatg ctccctggtg tggacattgc 1440 aaggagctgg ctcccatctg ggagcagctg ggtgaaaaat atgcagatca tgatgacatc 1500 atcattgcaa agatggacgc cacagccaat gaagtggaga gtgtggcagt tgatggattt 1560 ccaacattga aatttttccc agctggtggc aaagaggtaa ttagctacac tggaaacaga 1620 gatctagaaa cgttttccaa gtttcttgac aacggaggag tgctgccaga agaggaaagt 1680 gatgaggacg atgacgatga agaagaggtt aacaaagatg aagctaagcc aaatgaagga 1740 gatgacgctg atgatcagag caagcagaca gatgactctt cagagggacc aacaaacaac 1800 acatccaaag acgagctgtg atgacaattt aaactcaacc actctgtcat ttttcttaat 1860 aaaatagtat tcaagttaaa aaaaaaanaa aaaaaaaaaa aaaaaggccg cctcggccac 1920 tctgcgttga taccactgct t 1941 <210> 78 <211> 980 <212> DNA <213> Oryzias sp. <400> 78 ttgttctggc caagatggca tccgcccgga agagggctca gacggtgggt ttcagcaagt 60 ttgccgtggt caatcttcgg gatggagttc tgtgtctgtc atggcgcctc ggggacttca 120 gagggaacca tatactggag ggggtggtca cagctcagct ggtccggtgc atgaggcggc 180 cgcagggttt catcgcgtgg tcataccagg atctgaacat agaagagcca gaaattgccc 240 tcaccacacc agctactatc atccacaagc tggaaccagg aagtccgctg tacagcttgg 300 atccggacga gctcctggag gagaactttg aactggtcgt ctctttcacc tatacaggag 360 actcgacggg catgctccac cagacacgca ccacctacac accggcagac atccgctggg 420 gtcagcgctt caaggacatt ttaaaagtgg gcaagaagca ctacaaggtg gactacgctt 480 tcttcaacga gaccacatgg gtgcaagtgc ccatgatgag cgcagagtcc tttaacagga 540 agcgacacct tgaagacggc cggctacacg tgtcatcggt caaaagaaaa ggcggcactg 600 aacacgtgac agtagaaatc actgaagaac cagtgttgca gacatttttt gtaacatttt 660 gagtttgagg agtcctattc tttaaaatgt tgccccatgt gagaagttcc catttcccta 720 gaactttcat tttgcaaacc gatgttattt tcctcagatt tcacatcatt gttcacacac 780 acacacaaaa aaagaataaa cactgtagaa cacttgtatg tattcaaaaa tggttcaaaa 840 tgtacttttt agaggctttt atctgaaaca tgttatcttt tgacatggca taacataaag 900 aaatgtggat cataccatat tgtgtaggag tggggggaca acttcagact tgtacatttt 960 tctatttaga tttcagatca 980 <210> 79 <211> 431 <212> DNA <213> Oryzias sp. <400> 79 aagcagtggt atcaacgcag agtggccatt acggcctggg gaggtccacc tggacgacct 60 gaaccgcagg atctgctaca gttctcatgg agcctacgcc cagagcaagc tggctctggt 120 cctcttcacc taccacctgc aggagcagct cagcgccggc ggcttccccg tcaccgtcaa 180 cgccgtggac cccggcatgg tggacacggc gctctacgat aacctgtgga ccctggcgca 240 ggtgctgaag aggccggtgg ccaaggttct attcaggact ccggcagagg gcgcctccat 300 ctccgtgtac ggcgccgcgg ccgcagagct ggagggcgtg ggcggctgct acctgtacaa 360 cggtctgaag acccggtcct cagagctgtc ctacgactgc cagctgcagg agcagctgtg 420 gaagaagagc t 431 <210> 80 <211> 1876 <212> DNA <213> Oryzias sp. <400> 80 aagcagtggt atcaacgcag agtggccatt acggccgggg actctttcat gagccatcat 60 aaccaacagc tgtccgtcta gaatggggaa agttcatcag gaggatagca atgtcaaaga 120 gaagaacagt gagttgagct tggtgagggt caaaggtaca tggaagagca aaacgggctt 180 gaagatcaag gacgctgttg actgtgtttc tgctgctaaa atatatgaaa gcatctcaga 240 gcaaggcact acttggacag tggtcagccc catcaccttt acctacaagg tcactgagac 300 tcatagttta ctggatccaa gcaatgacac tcttctactg ggccacatca ccgacccaca 360 gcaaatggag gacattaaac gctcaaacaa gcccatgagg cgatttgtgg tcgtcgatca 420 ggaagtctac aaaatctatg gtcccaagtt aactgaatac ttagaagcta atgatgtggt 480 ttacaaagtc ttggctcttc caaccacaga ggagaataaa tccatggaaa ctgcactgaa 540 aatcctggaa gaagtgaaca acttttccct ggaccggcgc acggagcccg tcattgctat 600 tggtggaggg gtttgcttag acatagtggg tctggctgcg tcgctgtaca ggcggcgcac 660 tccgtacatc cgggtcccaa ccaccctcct gtcctacatc gacgccagcg tgggggcgaa 720 gacgggcgtc aattttgcaa actgcaagaa caagctgggg gcctacatcc caccagctgc 780 tgccttcctg gacctgtcct tcatgcagag tgtcccgagg agacacatct ccaacggtct 840 ggctgagatg ttgaagatgg ctctgatgaa gcacaagggc ctctttgagc tcctggagga 900 acacggctgt atgctcttag acaccaagtt tcagactgga aacaatgtat atggacacaa 960 cagtgtgaag gttgcatctc agacaacccg aatagcaatt ctaaccatgc tggaggagct 1020 ggctccaaat ctttgggagg atgacctgaa cagactcgtt gactttggtc atcttataag 1080 tccagcacta gaaatgaaag ttctcccgtc cctgctgcac ggggaggcgg tcaacatcga 1140 catgtcctac atgacttacg tgtctcacga gagcggcctg ctgacggagg aggagaagca 1200 gaggatcatc agctgcatgg tggggctgga gcttcccgtc tggcacgagg agttcaccat 1260 ggagctggtc cagaagtccc tgcaggacag actgaaacat tccggcggcc tggtccgaat 1320 gcctctacct gttggtctgg gacaagcaga aatatttaac aacatttcat acgacatcct 1380 ccagaaagct tttatcaagt ggtgtgaaga gctgagcgtc tcctctgata gcacctgtga 1440 cgcttgagct tcagcttttt aaagaaacgg gtttttattt cagctactac gtttggtgtt 1500 tggaagtgta atgttgttca agcagtttgc tgtggttaaa gtctgatgtc caacaatgat 1560 aaaatgagat ggttccactt ccactgatgg agactgtaaa tatcctttct gaaaaacaca 1620 ttatagcagc tacttctgtg agtcatgaag acaaatgatg acagcatttc ttcactttgt 1680 tttacagttt cagaatagct tgaatggaat aatcaagaaa tgtttttaag tggtttaaca 1740 ctaatttaca ttaaacagtt gaagtgttat ttgggggaaa aaatggatta aaggaaccag 1800 aagttagttt ggtacgttct tgtttgtgct taaaaaatgg aaacaaactc cattaaaagt 1860 tttttattaa cagtac 1876 <210> 81 <211> 685 <212> DNA <213> Oryzias sp. <400> 81 aagcagtggt atcaacgcag agtggccatt acggccgggg agaaacagat tcagcatcag 60 acggtagaag actcagatcg tcaaaatgaa gattctggtg tttctgtgtt tggtgggatt 120 gagttttgac ggcgctactg cagtgacgca ttctctgaag tatttctaca ccgcttcctc 180 tcacgtccag aacttcccag agtttgttgc tgtcggtttg gttgatgatg ttcagatgat 240 tcactatgac agcaacacca gagtggcggt tcctaaacag aactggatga aggagggtgt 300 ggatcaacag tactgggaga gtcaaactgg aggccttctg ggtgctcagc agagcttcaa 360 agccaacatt gaaatagcaa agcagcgctt caaccaaact ggaggtgttc acgtgttcca 420 gaacatgtac ggatgtgagt gggatgatga gactgaagag gtcaaaggtt attatcagtt 480 tggttatgat ggagaagact tcattaatct tgacctgaag gatgactcat ggactgctcc 540 aaaacagcag gctgtcatca ctaaacagaa gtgggataat gacaaagctg acatggcata 600 cacaaagaac tacttgtctc tggagtgtcc cgagtggctg aagaaatact tgaaatatgg 660 aagttctttg aaaagaacag tgtct 685 <210> 82 <211> 994 <212> DNA <213> Oryzias sp. <400> 82 aagcagtggt atcaacgcag agtgcccatt acggccgggg tttcatcagt tttttcagaa 60 agagaagaga gctgggctcc tgatctcagc tgagtcatgt ctggactcaa tgcgtccctc 120 ggatacttcc tggctgtcgt gatttttgca actttgctgc gaatgctttt caacaaatgg 180 ccacgtctcc atttcatggc agagtttgcc tcttctttca tgctggtggc atgttggctg 240 gaggttcaga ccatagtgga ggtgggcgag tgggctgggg gtctgggtcc tgatgtgact 300 gccaccatgc tgcttgtggt gctgctgact catggtgtga tcagtggggg ggcatcgggg 360 aaccccagct tggctgtgca gaagttcctg cagctggagg acagccccct gcccaccctg 420 ctgacggtgg cagcccagtt tctggggtct cacgcgggtt tccttgcagc agggttttac 480 tggaggctgg agctgacgga catgcacatg atcaaaaacc tgatgtccag tgagtgcagt 540 acttccctgc aggtccctgt gctgcagggc ttttccactg agttgctctg tgcgatcgtc 600 tttcatctcc tctgcctgag cctgaagcgc agatctgctc tgattggcct tccgctggtg 660 gctctcctcc gaacattgct gtctcattct gcccgacgct acacttcagc ttacatgaac 720 ccatctttgg cgtacggcct caccttccac tgtcctggat tcaccttcac agagtatgca 780 gtggtctact ggctgagctc tctcacaggc atgactttgg ctcttctgct gtacatgggc 840 cacattccca ggatgtttac caaaaacctg ctgtatctcc agaagacacg cttcagagtg 900 cccaaaggag acaaagcaga gaagaaaaag atgtgaacga tggagaaaac acactcaatc 960 attaggatgc atttatttta gacaaagtgt aggg 994 <210> 83 <211> 865 <212> DNA <213> Oryzias sp. <400> 83 cgttgacaag ctaatattaa taacagtgag aatcagttgc cctatgctgg tgaagattag 60 tttaaaaaac gaggggggac ggcatccaga ggagagcctg atggtttcat gtggttgaca 120 atgtcttcaa gaaaagacag agtcacaggc tcgaacgact ggagcacaga tcgaggaggg 180 ataaaatcag atggatcaaa gccgggtgct agaataacag atctgatgtt agcaaccttc 240 tgagtaaaaa agtttaaaaa ttcatcacac atttcagaag atgcttcctg gcagtcagac 300 tgtggggtgt taagaacaga ggtgacagtt ttaaaaagca cacgtggatt gtggctgtta 360 gtctcgataa tattggacaa atatgcttca cgtgattttt tgacagtgat cagataatga 420 tgccaacagt cctttaaaat ctggagtgaa acctgtagct tgtccttttt ccacctccgc 480 tccgctctac gagtctccct ccttgcctgc cgggtgctct cattaaacca gggctccggt 540 ttggccctgg gtttcatggt tttaaatgga gcaacagaat ccagaacatc caggcaggag 600 gagtgaagaa tacgtttatg tttatatatg tttatttttc tacatctgct aaacctaagt 660 cgtatgctct gtaaaaggta ctttgttttc aaagaatgtc aagcttcatg gctttaatct 720 tgtcgatcat tttttgttct tttaattatt ttcttttatt ttttgtgaaa acaagggact 780 tagtatgtta agacactaca gattttaaca ttggggtgta atgcctctgt attgaaaaaa 840 aatgtttagt ctttctttgt aacta 865 <210> 84 <211> 2216 <212> DNA <213> Oryzias sp. <400> 84 ttacggccgg ggatcattta acacagcaaa gaacacagaa aggagaatag ttgagagaat 60 ctgcgcagaa aggaagaggc ttctttcagc tgagactacg cgaaaggcca tcgttttact 120 gaaaaaatat tagcattggt tttcgtagat ctgtgggcat acgcgcgaaa gatgaagctg 180 ttgtgggcgg taatgctggt agtcggcgtc gtgttcgccg aggaggagga caagaaggac 240 agcgtgggaa ctgtgattgg aattgacttg gggactacct actcttgtgt tggagtcttc 300 aagaatggcc gtgtggagat cattgctaac gaccagggta accgcatcac tccctcctat 360 gtggccttca caagtgaggg agagcgtctg attggtgatg ctgccaagaa ccagctgacc 420 tccaacccag agaacacagt ctttgatgct aagaggctga tcgggcgtac ttggggtgac 480 tcctctgttc agcaggacat caagtatttt cccttcaagg ttattgagaa gaaaagcaag 540 ccccatattc agattgacat tggtggtggt caaatgaaga cttttgcccc tgaggagatc 600 tctgctatgg ttttgaccaa gatgaaggag actgctgagg cctatctggg aaaaaaggtc 660 actcatgccg tggtcactgt tcctgcatac ttcaatgatg ctcagcgcca ggccaccaag 720 gatgctggaa ccattgctgg cttgaatgtg atgaggatca tcaatgagcc aactgctgct 780 gcaatcgctt atggtctgga caagaaagac ggcgagaaga acatccttgt gttcgacctt 840 ggtggcggca ccttcgacgt ctcccttttg accattgaca acggtgtctt tgaggtggtg 900 gccaccaacg gtgacactca tctgggaggt gaagacttcg accagcgcgt catggagcac 960 ttcatcaagc tgtataagaa gaagaccggc aaagatgtgc gcaaagataa ccgggctgtg 1020 cagaaactgc gccgtgaagt tgagaaagcg aagagggctc tgtctgctca acatcaggcc 1080 cgcattgaga tcgagtcctt ctttgaggga gaagacttct ctgagactct cacccgtgcc 1140 aaatttgagg agctgaacat ggatctgttc cgttctacca tgaagcctgt ccagaaggtg 1200 cttgaggatg ctgacctgaa gaagtctgaa attgacgaaa ttgtgctggt tggaggctcc 1260 acccgtattc ccaaaatcca gcagcttgtc aaggagttct tcaatggaaa agaaccctct 1320 agaggcatca accctgatga agctgttgca tacggagctg ctgtgcaagc tggagtgctc 1380 tctggagaag aagacactgg tgatgtggtt ctcctggatg tgtgccccct gactcttgga 1440 attgagacag ttggaggagt aatgaccaaa ttgatcccca gaaacactgt ggtgccaacc 1500 aagaaatctc agatcttttc taccgcctct gataaccagc caactgtcac cattaaagtt 1560 tatgaaggtg aacgtcctct gacaaaagac aaccatctgc tgggcacttt cgacctgacc 1620 ggcatccccc ctgcacctcg cggcgttcca cagattgaag tcaccttcga gattgacgtg 1680 aacggcattc ttagggtcac tgctgaagac aagggcactg gaaacaagaa caagatcaca 1740 atcacaaacg accagaaccg tctgacgccc gaagacatcg agcgaatggt gaacgacgct 1800 gagcgcttcg ctgacgaaga caagaagctg aaggagagga tcgacgctcg caatgagttg 1860 gagagctatg cctattctct gaagaaccag attggtgaca aggagaagct tggtggcaaa 1920 ctctcagatg aagacaaaga ggccattgag aaagcagtgg aggagaagat tgagtggatg 1980 gagtcacatc aagatgctga gacggaagat ttccaggcca agaagaaaga actagaagag 2040 gtggttcagc caattattag caagctatat ggaagtgcag gtggtccacc accagagggc 2100 gctgagagtg agggagagaa ggatgaattg tagggaggtt tggcctcatt tgttgcctct 2160 tgccaccatt acagtggctt ctgcttatga aaataagact gatgggactc gatagt 2216 <210> 85 <211> 241 <212> DNA <213> Oryzias sp. <400> 85 attctagctc tcagtttctg ttgtctagat cttgctctca tctcctcacg cgtcctcagg 60 tgtctgatcg tctacccctg gactcagagg tacttcggca gttttggaaa cctctacaat 120 gccgctgcca tcagctcaac ccaaaggtcg cggcccacgg aaaggtggtc ctctcgggtc 180 tgcagttggc cgtgaataac atggatgaca tcaagcagac ttacgctgat ctgagcgtgc 240 t 241 <210> 86 <211> 900 <212> DNA <213> Oryzias sp. <400> 86 aagcagtggt atcaacgcag agttggccat tacggccggg gacgactggg cgaaaatggc 60 ggagtatctg gcatccattt tcggcacaga gaaagacaag gtcaactgtt ccttctattt 120 caaaattgga gcttgcagac atggagaccg ctgctcgaga ttgcataaca aaccgacctt 180 tagtcagacc atcctgatcc agaacatcta ccgtaatcct caaaacagcg ctcaaacggc 240 tgatgcttct cgtgccgtca gcgatgtgga aatgcaggaa cattacgatg agttctttga 300 ggaagtcttc acagagatgg aggagaagta cggagaggtg gaggagatga acgtgtgcga 360 taacttgggc gaccacctcg tcggcaacgt ctacgtgaag ttccgccgcg aggaggacgc 420 ggagaaggcg gtcatggacc tgaacaaccg ctggttcaac ggccagccca tccatgcaga 480 gctgtcgccc gtcaccgact tcagggaagc ctgctgccgt cagtatgaaa tgggagagtg 540 cacccgcggg ggcttctgca acttcatgca tctgaaaccc atatccaggg aacttcggag 600 ggagctgtac ggccgccgca ggaaaaggca ccgctctcgc tcacgctccc gcgaacggcg 660 ctcccgctcc agggatcgac gacgggaccg cgagaggcgg aggtcgaggg accgagaacg 720 ctccggacgc ttctagagga aggccggata gatccaccga acccctccct cctaacacga 780 cagtgacaac gttttcattt gtttttcttg gtgaattgtt ggacgtgttt gtttctttta 840 ttcccttttt taagacaagc ttgtgtcgct ttctcaataa aacagatttg caactctaag 900 900 <210> 87 <211> 1389 <212> DNA <213> Oryzias sp. <400> 87 ggagacctgg ggaaatatga ccaggacaac ttcctgtaca tcacagggag gatcaaagag 60 ctgatcatca ccgccggcgg cgagaacatc cctcctgtgc caattgagga cgcagtgaag 120 agcgagcttc ccatcatcag caacgccatg ctgattggag acaagctcaa gttcctctcc 180 atgctcctca ctcttaaatg tgtcatggat gataacggcg agccaaccaa tgagctgagc 240 tcagaggctt tggacttctg ccggcatcac ggagttaatg ccaccaaggt gtcggagatc 300 acagccaata aggagcctgc tatctacaaa gctattcagg aaggcataga tcgcgtcaac 360 gcaagatcaa cgtccaacgc tcagaggatc cagaagtttg tcgtgctgga gcgagatttc 420 tccgtcgttg gaggagagct gggacccacc ttgaaactgc ggagacccat tgttgtgaaa 480 atgtaccagg aaaaaataga tgagatatac tcaggagctg cagaaaaaca gtagactgca 540 ctgcactgca ctgcactgtt cggagagccg aggcgctcat gggaaatgtg gccagaaaca 600 gaggagaagt ttgtcttgcc aaaagctgtg aggatgtctc tctttgcttt gaatgtaaat 660 ctacgactta cagtctgaca ctggcagcac agaaaagatc gcaaagcaga gcaaattttt 720 ttattttttc ccatgcgaaa cacggaggtg ggagtttcat ggcttctaaa gaaaaaagga 780 cgggacggtg caactaaaac gagttcaaat gtttacttag aagaaaagca ttcacaacca 840 ttccagttta tgttttctgg tgaagtacat ttgaatgcag cccaatgttt aaatctttca 900 tcacatggtc cccatgacaa cctggagcaa agaaacgaat gtcagctgtc acaacaagtg 960 caatgaagaa ttatcctgta tgtcggaaac gtttttttga aatatttatt tagtggtaga 1020 ttcgtcttca taaagtgaaa aaaaggctga atgttgacat gtactttatt ttgtttaagt 1080 catcacattt ttgtctgtat cagcacaaac aatgcatatc aaggggtaca ctgtctgtga 1140 aactcataaa tcatgtcaaa gcaatgaata agcaaaaata ttcatcaacg gataattgct 1200 gccactttaa ataaatgtaa agaatttttc tacgtataaa tctgattcta aatcgatttt 1260 taagattgtt ttaatcatat atatttggtt atttcaacta tttaaaaaat gattttaatt 1320 gtgttcttta aaaatgagaa aaaatgattg tgtttttaaa gaaattaaat aaatgaagtt 1380 tactaggtt 1389 <210> 88 <211> 674 <212> DNA <213> Oryzias sp. <400> 88 aagcagtggt atcaacgcag agtggccatt acggccgggg gacagttacg tcccagcaga 60 atcctttcca acactcgagt cagaatgtgg ggtctgctcg ccaccgtggc ctcgctggcc 120 gtgctgtctc tgggggtttg tgaggagaag gtctttctgc agcgagcggg cacggcctac 180 aacagcagaa agtaccgatc tgagctgaac gtgaccaacg gagagcgctt cggaacctgg 240 acctggcccg agatgtgtcc ggagaactac tttgctgttg gattcagtgt tcgggtggag 300 tcaaaacagt acggagggga cgacaccgcc ctgaacggga tccgcctcat ctgctccaaa 360 gacgagaacc ggaacttcct gtattacgtc gaatcgcaca ctggacactt cggtgagtgg 420 tctcaccctc agtactgccc cggcggcgtg ctgacctcct tccagctgcg cgtggagtcc 480 taccgcgggc ctctgggaga cgacacggcc gccaacaaca tcaagttccg ctgcagcagc 540 aaccccacgc tggaggctcc cggcatgagc tggggggata cggctactgg agccaggact 600 gtctgggggg agggatctgc ggcatcgaga ccaagatgga ggagtaccag gttggcctgg 660 acgacaccag cctc 674 <210> 89 <211> 1275 <212> DNA <213> Oryzias sp. <400> 89 gaggtcggct gcttactcta aaagctaaac acaagataaa ccccgtacgt gcaaggatta 60 aagctcacac tttgtgaaat aaacattcat tttaaagcta gcagaatgga aaagccgcac 120 attgtcacgt gtactgcccc tgtgaatata gctgttgtta agtactgggg aaagagaaac 180 gaagaattga ttttaccaat caactcatct ttgagcgtca cgttgcatca agaccagtta 240 aaaacaacga caacagtagc aaccagcaaa tcgtttaagg aggatcgaat atggctcaat 300 ggcaaagagg aggatgtcac ccatccaaga cttcagtcat gtctcagaga aatccgacga 360 ctggcacgaa agaggcataa caatggagac accacttcaa cagacttgac tggtctttct 420 cagaaagtcc acatttgctc tgtaaacaat tttcccaccg ctgccggact cgcctcgtct 480 gcagcaggct ttgcatgtct agtttacagt cttgctcagg catttggtgt cgaaggagag 540 ctgtcaggta tcgctcgtca aggttctggc agcgcctgtc gcagcatgta tggagggttt 600 gttcagtgga tcatgggaaa ccaggaggat ggaaaggaca gtttagccca gcaggtggag 660 ccagagagtc actggcctga actcagaatc cttgtacttg tggtcagcgc tgagaggaag 720 ccagtcggca gcacttctgg gatgcaaact agcgtacaaa caagttgcct actaaagcat 780 cgtgcagagt cggttgttcc tcaccggatg gaggagatga tcaaagcggt gcgaggaaaa 840 gacttcgctg cgtttgctga attgaccatg aaggacagca accagtttca tgccacctgc 900 ttggacacat accctccaat cttctacctg aacagagtct ctcaacaggt catccacctg 960 gtgcatcgat acaacagaca ttatggggag acgcgggtgg cttacagctt tgacgccggg 1020 ccaaacgctg ttattttcac tttacaacag catgttccag agtttgtcca catggtccag 1080 catttcttcc caccagaaac caatggagaa gacttcatca aaggtcttcc agtcaagcat 1140 acccccgtct ctgagcagct gaagcagagt gttggtttag agcccatgcc aaagggaata 1200 aactacatta ttagcactaa ggctggtcca ggcccacggg ttgtgaagga tccctctcta 1260 cacctgctgt cacct 1275 <210> 90 <211> 1411 <212> DNA <213> Oryzias sp. <400> 90 aagcagtggt atcaacgcag agtggccatt acggccgggc agttgccaga taatgttatg 60 gagaatcttc ctccagctca agccaatttg gaatacttca catttaaaga acaggaagtt 120 agcgtttcga taggcacccc tccattgacg agaactattt ccttgaccaa aggactacgc 180 aaactcttct tattccctca gacatcaatg aagaatgggt gctgattgag gatttaacct 240 ccaagccaga agagggaaac aatgcaatcc gatttgtgaa cggactggcc ggagacctca 300 acgtctcaac ttctgcagtg gactttggaa tcattgagcc attcatgtct tcaaactact 360 cgctgttgag aaatggacaa cacaccttca gcttgatgag tggatcacag tcatgtgaat 420 acaccagaaa atttggattt gggagttcat acactttctt cattcccagc actttcactt 480 tcggaccaaa ttgtcaggaa tctttcacag aagttgagga aataaagccc aacacggttc 540 acatggccct ccagatccct cagtactttc taatcactgc aggtgaagtg atgttttccg 600 tcaccgggtt ggagttctcg tactcacagg cccccagtaa catgaaagca gtgctgcagg 660 cgggctggct gctaacggtt gccatcggta actttatcgt cctgattgtt gcagagatcg 720 caaagcttcc taaaaagtgg gcagagttct tcctttttgc atccctgctg gtggcggtct 780 gcgtcatctt ctccatcatg gcttacttct acacttacat cgaccccacg gagatcgaag 840 ctcagttcaa ggagaagaag gaagacagcg aggaggagga ggaaaaagat gagatgaaga 900 aaactgaagt gcagatgatg aaaaaagact cgattaaaag taatgacagt gacgacggat 960 tcaaacaatc aaaaatgtaa aaagtagaac cccaccatcc aaaatctgca ataatgtcat 1020 agtaccggta atatatttgt ttaaactatt tttaacttta ataaacagaa gcatcatgag 1080 tgacgcacac ccacaatcag ttttcagagc tgatattttt aaagattaaa aaaaactaag 1140 gttgtccatt tattttattt ctgaatggaa tatttgtgta ttcatactaa ggtgtgttga 1200 agaaagcaat taattttttt atccaagttg tggtactttt tgtacattaa gtttacttca 1260 gttactagtc ctgcatcccc gctgttttaa aattaatttg tcataaagtt tcttcaaatt 1320 ccttgagtag aatatgtatc ttagtctttc tttgctttcc ccctaatcct tcctgactgc 1380 gtccacaggc ctcatcagcc cctgaatcag c 1411 <210> 91 <211> 638 <212> DNA <213> Oryzias sp. <400> 91 atttgatcca ctggattcta aaccacaaat atctgatcca ctggaatcta aacgtcaaat 60 atctgatcca ctggaatcta aaccacaaat atctgatcca ctgaccacca gtgaacacca 120 gtgaacacct ttttgcaaag ttgttgacat ttttgcaaaa tgtcaacaac gcctcttttt 180 tctacgtaaa cttcgtaaac tccaaattca tccgtccatc ctgacaacct tttacaaatg 240 tttcattgaa tccatacttt cattcaacat tgtcgcttgg tttggttcac tcagtcttgg 300 caatcgtaat gcaatcaaca gaataattaa aatcagcagt aaaattgtag gccaagaact 360 ggagtcactc atgacaattt tcaataaaaa agctaaacgt aaaggttcac acatcgccac 420 agacgccaca cacattcttc acacccatta cagcttgttg ccctcaggtc gcagactcag 480 atctctgcct ttcaaaacaa agagagctat gtccagtttt gtaccaactt caattcgcct 540 gctaaacagc taaagttgga aactctactt tagggagtct gcatcttaaa agggtatgtg 600 tgttgtatgt atgctactgt ttttattggt agtatttg 638 <210> 92 <211> 1373 <212> DNA <213> Oryzias sp. <400> 92 gtgtgttcag ggacgtgttt gtcgtctctc ctccgcattc ctgctcattc acagctcagg 60 cgtgtaactt tacacaacca actgactttc cagcatgctc cacttccagc cagcgccggg 120 tcagttcggc cccgaggtcc gagatccggg tctgtgggtg tggagggtgg agaagatgaa 180 ggcggtgccg ctggaccctt cccgaggtgg ggtctttcta caacggggac tcgtatttgg 240 tgctgaaaaa ccacggcgag cagggagcag acctccacat gtggctagga gagaagtcgt 300 ctcgcgatga gcaggttgca tgtgccatgc tggccaccca gctagacaac tttcttggcg 360 gtgaccccgt tcagcaccgg caggttcagg gctttgagac gccggagttc atggtcctct 420 tccccagagg agtcagctac aaggatggag gggtggagtc gggctttcgg aggactcagg 480 ggtctggacc ggtgcacagg ttgtaccaga tcaaggggaa acgcaacatc cgtgccaagg 540 aggtggagct gagctggagc agcttcaaca agggcgactg cttcatcctt gacctcggag 600 agattatcgt gtcgtggatc ggatcgcagg ccaacatctt cgagaaacaa aaggtgcgtg 660 aaatagcatc gttgatccgt gacacggaca gacacgggaa agccaggatc gtggacatca 720 ccgagggcga ggaaccggag gagatgctta aggtcctggg gcagaagccg gagctagcag 780 agagcacgcc agaagaagac agcaaagcgg acgcttccaa caccgctgct ctttacaagg 840 tgtccgatgc aaccggttcc atgacaatga ccaaaatctc agagaagagc ccgtttgcca 900 aggatctgct tgtgcgcgat gactgcttca tcctggacaa cggagccaac gggaaaatct 960 tcgtctggaa agggaacgga gcaaacgccg aggagaagag ggtggccctg cagatggccg 1020 acagcttcat caagcaaatg cagtacaaca tgatgaagac gcaggtggag attctgccac 1080 aagggaagga gaccatcatc ttcaagcagt tcttcaagaa ctggaactga agaggcagaa 1140 caatctccat gtcatttttt ttatacacat ttccacagaa gcagctctct ctggatttca 1200 gtggaatcgg agacgtgatg ccacttttgt aaaaattccc aaaattgttt catttggtga 1260 aattttaaag tatcatagga gttatttgtt aatatgcaga acaaaaactg tatagaggag 1320 caagatatta aacagtggtg caaatctact gacataataa accctggaaa gtt 1373 <210> 93 <211> 405 <212> DNA <213> Oryzias sp. <400> 93 gcaacgtggc gatgtacgac gccaccagcg ggaagctgga ggaggagctg cagggggtcc 60 acagcgagga gatcactgac ctcagatttg atattaatgg ccggtttttg gtgagcagcg 120 gcgacaaggc tatccgtgtg tttcacaatg cgccgggtta tcgggcggcg attagagaca 180 tgaatgacat gctcaaaaag gctcagaacg aagccatgaa gcagagactg cagcagcaga 240 tcaaagaggc tcagagcgcc ctggacttgg cactcgctgc tcctgctaac tgaaaagact 300 gtgaccctgt ttgatgtaac acttgtgctg taaggtgatg tggttgaaaa aaaaaggact 360 ttgcatgaga ttttaattta aaaaaatgct ctttttatgg attga 405 <210> 94 <211> 712 <212> DNA <213> Oryzias sp. <400> 94 attctagctg tcatccaggg aaagtccacc atgtcccagt ccaggagcca ccacctgtct 60 cccatgtacc tgtctgctgc catgctgctg gctgtgctca gctggcagta catcagcacc 120 gctgccgccc aggcgcaggg gcacgggggg acgtgtaggg gggcagtttc tcactcgtcc 180 tcacagaggt tcacagactg aagagcagac ctctgctggt ccatggaaat gcactaaatt 240 taaggattca ctatgactgt gaacaaactg gacactttga ccctagtggg gtgtattaat 300 ctggtcacac atggcttgta gatcacgatg gcaatgaaga aacttcactc agctttaggt 360 ttctcctcct ttatggcaaa tcttctctgg tttcatttct ctgatcagaa gtcagcaaac 420 ctaaccatct ttttacagag tcccagagat gtttttcaac actttttgca atccactcga 480 tctccgtaga ccacatgaga gtaaactgtg caataaacta gtttttatca agataaagag 540 gaaaacggag cgatgaactt aagttaaagt ctgttagcta agtgtcctta agtcggcttt 600 tgtgagtagt tcagcgtaaa tgtgtacaga gccaaaggaa agctgcaaac attgctatgt 660 aatattttaa tttgtacata tttgaaataa gaaataaaag tgattaagtt tt 712 <210> 95 <211> 1400 <212> DNA <213> Oryzias sp. <400> 95 aagcagtggt atcaacgcag agtggccatt acggccgggg gcactctact ggtcaacatg 60 agggggctgc tggcgttttc cctgctgttt gtagctgttc taagcaagga gacttttgtg 120 gggcaacaag tccttcgcat cgttccgaag gatgaagtcc aacaatctca gctcagaaat 180 ctggaggaga tgatggagtc cgagctggac ttctggagag gggtcacccg catgttcacc 240 cctgtggatg tcagagttcc ctcccacgcc ctgcagtttg tcaaagactt tctggggagt 300 cacaacattg agtactccgt cataattaga gatctgcagg cactgctgga tgaggagcag 360 gaggaaatgg atgcagcagc tctttctgga agtcccagga acactgacag cttcgactac 420 gcaaactacc acagcatcag cgagctttac agttttcaag acatgctggt ggctgagaac 480 cccaacctgg tcagcaagtt ggtgattggt cagagctacg agggccgtcc cctgaatgtg 540 ctcaagttca gcactggtgg atccaaccgc cctgccatct ggatcgacac tggaatccac 600 tctagggaat gggtcactca ggccagtggc acctggtttg caaagaagat tgtgaaggat 660 tatggaactg accctgctct cactgccatc ctcaacaaca tggacatctt cctggagaca 720 gtgaccaacc ctgatggata cgcatacact cacagctcta accgtatgtg gcgtaagacc 780 agaaagccca accctggatc cagctgcgtt ggagttgatc ccaacaggaa ctgggatgcc 840 ggcttcggag agcccggtgc cagcagcagc ccctgctcag agacctaccg tggacccagt 900 cctaattctg agtctgaggt caaggctatt gtggactttg tggtcgccca taaaaacatc 960 aaggccttca tctccatcca ctcctactct cagatgctct tgtaccctta tggctacacc 1020 tccacaccgg ccaaggacga ggctgagctg catagcctgg ctcagaaagc catcactgac 1080 ctggcttcct tgtatggaac tcgctaccgc tacggcagca ttatcgacac tatctaccag 1140 gcaagtggaa gtactgttga ctggacgtac gagcagggca tcaagtactc atacaccttt 1200 gagctgagag acaccggcta ccacggtttc ctgctgcccg ccaaacagat tgttcccacc 1260 gcagaggaga cttggctggc tctgatggca atcatggagc acacctacaa gaacccgtac 1320 taagtccaga actcccttca gggccccaca gcgctcaccg ttggggtgta atctgtataa 1380 ataaaacatg aatctttacc 1400 <210> 96 <211> 2112 <212> DNA <213> Oryzias sp. <400> 96 aagcagtggt atcaacgcag agtggccatt acggccgggg agaatgaggg atgcaaagcg 60 gctcgtgggc tgcgcacgtg tagcgcacat ttagaggttg ctttaggaaa acgttatctg 120 gttcttggat cagaagttta ttttagaata gagggactgt gaacaatttc tcggtttggg 180 cgaaactctt gaccggagaa gattcacgac taatgagtaa aatgatgaag tacatcctgg 240 tgactggtgg ggtcatctcc ggcatcggca aaggcatcat tgcgagcagt gtggggacca 300 tcctcaagtc gtgcggtttg catgtgacgg ccatcaaaat cgacccgtac atcaacatcg 360 acgcagggac tttttcaccc tacgagcacg gtgaggtgtt tgtgctcgac gacggcgggg 420 aggtggactt ggatttgggg aactatgagc gcttcctcga tatccgcctg accagagaca 480 acaacctgac caccgggaag atttatcagt cggtcattaa caaagagagg aggggagatt 540 acctgggcaa aaccgttcaa gtggtgccac acatcacaga tgccatccag gaatgggtgg 600 tgaagcaggc cagagtgccg gtggatgatg atcaagtaga acctcaagtg tgtgtgattg 660 agctgggggg cacggtggga gacatcgaga gcatgccctt catcgaggct ttccgacagt 720 tccagttcaa agtgaaaaga gagaacttct gcaacatcca cgtcagcctg atcccacagc 780 ccagcgctac aggggagcag aagaccaaac ccacgcagaa cagcgtcagg gagctgcgag 840 gcctgggcct gtctcctgac ctgatcatgt gccgctgttc tactgttctg gagacttcag 900 tcaaagagaa gatctccatg ttctgccacg tggagcctga gcaggtcatc tgtgtccacg 960 acgtgtcgtc catctacaga gtgcccttac tgctggagga tcagggtgtg gtgagctttc 1020 tgagcaggag actcaacatg cccatcgagg ccaagcccag gaagatgctg accaagtgga 1080 aggagatgtc cgacaggtcg gaccggctgc tggagcagtg ctccatcgcg ctggtgggga 1140 agtacaccaa gttctcagac tcgtacgcct cggtcattaa ggctctggag cactctgcac 1200 tggccatcag tcacaagctg gaggtgaagt acatagactc ggcgtgtttg gagccagcca 1260 ccctgcagga ggagccggtg aaataccacg aggcgtggca gaagctctgc agcgccgatg 1320 gaatcctggt tccaggaggg ttcggggtga gaggaacgga aggaaagatt cacgccatca 1380 cctgggccag gaagcagaag aaacccttct taggtgtgtg tctggggatg cagctggccg 1440 tctgcgagta tgcaaggagc gtcctggagt ggacagatgc caactccaca gagtttgacc 1500 cggacaccag gcatcctgtg gttatcgata tgccagagca caaccctggg cagatgggcg 1560 gcaccatgag gctggggaag agacggacca tcttcaagag cggcagcagc gtcctgcgga 1620 aactttatgg agatgcagag tatgtagagg agaggcaccg gcatcgcttt gaggtaaatc 1680 cagagctgaa gagtcacttt gaggagaaag gcttccggtt cgttggtcag gacatcgaag 1740 gggagaggat ggaggtcata gagctggacg atcacccgta tttcgtgggg gtgcagtacc 1800 acccagagtt cacgtcccgt cccatcaaac catcgcctcc ctacctgggt ctgctgctgg 1860 cggcagcagg gaagctacag tcgtatttgc tcaaaggctg ccggctttct cctcgggaca 1920 cgtacagcga tcgcagcggc agcagcacgc cagactccga gatttcagag ttgaagttgc 1980 cgtctctggg gacagattct gcgtgaagac gaattcctca ggagggagca gctgctcctg 2040 tcatgtggcc ttgtgtgatc atgtatttta agcatgtttg tagaatattt caataaataa 2100 gattcacaca ag 2112 <210> 97 <211> 1201 <212> DNA <213> Oryzias sp. <400> 97 atagtgggct tgcactttga tcaggaggtc aatcgctaca aagggaagaa ctacccgatc 60 atgaatatcc atgagagaac cctcagcgtg ctggcttgtc gatatgtatc agaagttgtg 120 attggtgcgc cattcgccgt cactaaagac ctaatagacc atttgagggt ggatcttgtt 180 tgccatggga agacagaaat atatcctgac aaggatggat ctgaccccta tgctgagccc 240 agaaggaaag ggatcctgcg catggttgat agtggaaaca gcctcacaac agacgccatt 300 gtgcagagga tcatcaaaaa caggttgctt tttgagacaa ggaaccagaa gaaagaggcc 360 aaagagatcg ctgtgatcca agcaatgaaa cagaaagaag aggaaaagac taaagagcga 420 gcccaagctg tgctctaagc acttccacta catttgttaa cactgacatg cctagtttat 480 ataactccag tgaaatcaac tattagaatt tggcttacta tagttttgtt ttgttttcgg 540 atgttttaag ttcctcaatt atctcttcca gaggaatttg ccttaaacaa aataaataac 600 ttttttatgg gctttgagta aaatgaacac gcttgaaatt tttgtttagc ccaacaggaa 660 atcaatctgt atatctaata tatctttgag atgataaact actataagac ttgaatagat 720 ctccctgatt gtctgtggac acaatgtaca gtatacccaa agcatattat taaaattgtt 780 ctaatttaat aggaatttat ttttttaacc taaaaggcac attgtggaga cctcaaagtc 840 atgcttaatg agtgcaattg ttgtttttaa tcaatatttg aaatattgac actactgaga 900 aaaggtattg ttgtcatgac ggttgtatgc cacaaaaata tgagtgtaaa atgtaactaa 960 attatatata ttttagaatt atattatgaa ttttaacaat atgatataca cataaataaa 1020 tatacacata catttttatg tagaaatata tattttgttc acctccttct aaacgtgttt 1080 agcttgtctc atgtaaaaca gaacgctctg tgttgaaatt cttgtaggct ttgatgcata 1140 tgttacagaa tactcccatt ttaataaaat gtagaaaata attggattgt gatatatgat 1200 t 1201 <210> 98 <211> 484 <212> DNA <213> Oryzias sp. <400> 98 gaggagttcc tgacacgcag ctggcggccg ccgtctttct gtctgactgg accggggcaa 60 agctaagcta ccactgtaaa gttccgggga agcacacccc tcctgcttac ctggtggatg 120 ccgtggtcac ccaaatgcag agtggctggg atttgaagaa actgaaagca ggaaatgagg 180 aaactctgac aggtgttaaa tttccaaacc ctgcgagcag catatgcttc atctctaaag 240 gtccaacttc tggtgttctg ggtgtttctg aagaccaacc cggtgctgca aacagagcgg 300 aggagaagca gaacgaggag tctgtggact cgaatgaagc cgtggagaat ccagagaaaa 360 catcacagag caaaaaaccc aaaaaagtgc agtttcagtc atcgaccttg gaggtgaatc 420 tgacggctac gagtacagac gacgcctacg actttaacgc ccattttaaa tgaacttcag 480 ctcc 484 <210> 99 <211> 468 <212> DNA <213> Oryzias sp. <400> 99 ctctgcacat aggctgaagg ctatggaccc cagttgttag ctgcggtgcc ccctagtgcc 60 tctgtcccag accagccgct tggtgagccg cctgattgaa atcctgccag aaaccacgcc 120 cagtactctc tggccttaca cctcctggag gctgtggacg tcctcatcac ggaggaggac 180 tgaagagccg ggtcctggtc tgttatacag tcatcctcat catgtctctg tcatagaagc 240 tgcttttaaa ggtttacttc atcaggttga gtgcatgaat cagaatctgg ttgaatctgt 300 gggttaagca gagcgttgtc atagaagact tggtcctctc gtgccacatg agtttagttt 360 ctctgctctc tgttagtcaa catgtaaata aagaagagcc ttatttttac ctcgcctgtc 420 cctctatgcc caactgcttt cactaagaaa taaagaggct atgagaac 468 <210> 100 <211> 360 <212> DNA <213> Oryzias sp. <400> 100 aagcagtggt atcaacgcag agtggctcag gtgacagacc tgcaggcggc tgtggtctac 60 ttggcatctg acacctccga ctacatgaca gggcataact tagtcataga gggtgggcag 120 agtctgtggt agaaggaaaa acaacttttt attctttggt ttctggatat cctctcagaa 180 atactccagg aatcgttctt ttaggaagac ggaatgatga aatgtgacca attttctctg 240 cttgtatttg atccttgaat catttattta ttttatttac ctttatttca tcaggcaaaa 300 acattaagaa caatttctta tttacaatgt tggcctgagg aaaagattaa atatcttttt 360 360 <210> 101 <211> 833 <212> DNA <213> Oryzias sp. <400> 101 aagcagtggt atcaacgcag agtgccatta cggccggggg ctcgtcccag ccgtgcaatt 60 gtcctccaac aaaatgagac ttttctcctg ccttttgttc atcactgctt ccttcatggt 120 ggaaggggct ctgtccacga ggacggtcat cttcccttat caaacaagcc acagttatgt 180 ggagatcatt cccatgaagg agatggagct gggggctttc actctgtgca tgcaggtggc 240 cacggagctc gccggcgctc ggaagatcat cctcttcgcg tacggaaaga cggacgatga 300 gctgaatgtg tggcgggagc tgaacggcgg gatctccctc tacctgggag atggttacag 360 cgactccttc aaagttcctg accccggccc cctgaactct cacctgtgtt tgacctggga 420 ctccagaaca ggcgcgacca ccgtcttcat ggacggcagg aggagtctga ccaaattcct 480 ccggaagggt cacgtcatac ctgccggggg caaagttttc ctggggcagg acccggacga 540 cgttgaacaa atgcagtcgg gcttcgatgg tgaagagtgt ttggtcggag aagtctccga 600 cgtccacctg tgggactctg tcctgtcaga cggggaaatc cgagacttgc attctgggaa 660 atcagtgctg aagggaaacg tcatcaactg ggaaaccgca aacctcagag aacgtggaaa 720 cactgagatc ataacccggc gggtgtaagt ccggggtcat cggtcctgct gcggcccaga 780 ttgaacggct ggagcaaata aaatgaaatt taaaaagcaa aaaaaaaaaa cgg 833 <210> 102 <211> 256 <212> DNA <213> Oryzias sp. <400> 102 aaaatatgaa ttccgttatg tatttgtaga gatagacacc taccccagac ggactattgt 60 tgtggaggac aacagatgag tttttcctac actaagaagt ttgcatttgt aaagcgggtt 120 tttatggctg tgaactaaat acattgcttt tcttgaatat tttgttgtga aattttaata 180 aaaatattgg ttaattgaat tattgcccag catttggtca aaaaacttct gtcacagtgt 240 caaattaaaa aaacat 256 <210> 103 <211> 1819 <212> DNA <213> Oryzias sp. <400> 103 aagcagtggt atcaacgcag agtggccatt acggccgggg cagttggatc ctgacgacca 60 tgcttgggat tttggttgcc gttgccgtgt ttttgcagac ggtctctgct gcctctctcg 120 gtgtcgtgaa caccgaggga gggagggtgg agggcaaaac catccgcctg gggttatttc 180 gtaccatgga cgtcttcaag ggcatcccct tcgctgccgc tcctggacgg tttgagaagc 240 cgaggcctca cgatggatgg gacggtgtta tgaaggccac tgagttcaag agcaggtgtc 300 ttcagctgga aattaccttg gttggtacct atggcagtga agactgtttg tacctcaaca 360 tctgggttcc ccacggctcc tcagtatcca ccaacttacc tgtcatggtc tggatctacg 420 gtggcggttt cttggctgga gctgcatcag gtgccaactt cctggacaat tacttgtatg 480 acgggcagga actggcggac agaggaaacg tcatcgtggt gacgctggga taccgtgttg 540 gaagcctggg cttcctgagc accggagact ctgaacttcc tggaaactat ggtctgtggg 600 accagcaggc tgccatcgcg tgggtgcaca ggaacattcg gtcatttgga ggagacccca 660 gcaacatcac cgtcttcgga gagtcagcgg gtggagccag cgttaacttc cagattctca 720 ctcctcacaa caaagggctc atcaggagag ccatctccca gagtggggtt gccctttgcc 780 cttgggcaat taaccacaac ccccgcaagt atgctgagga ggtcgccctc agagtcaact 840 gccccaccga ttccagaatg gctgcctgtc tgaagatgac agatcctgtg gccttgacaa 900 aggcgggaac tattcatctg tctggctctc ctgatcagcc cattgtgaac aacctggttc 960 tgtcccccgt gatcgatgga gactttctgc cagatgagcc tcacaacttg ttccacaacg 1020 ccgcagaagt cgactacatt gctggagcca atgatatgga cggacatatc ttcactggtt 1080 tggatgttcc atctatcaac aatgatttag ttgaaactcc cattgatgat gtgaagaggc 1140 tgttggccgc cttgacaaag gaaaagggac agctgggttt ggagaatgct ttctccacct 1200 actcctcaga ctgggggtca aatcccagca agcaaaaggt gaagcaaact gtcgtggcca 1260 tcgagacgga ctacattttc ctgattccaa cacaaaccgc cctttatctt catgctgaaa 1320 aggcaacaac tggtcgtacc tactcctacc ttttctctga gcctaaccgt atgggtggcc 1380 tcattgcact ctaccccctg tggatgggcg ctgaccacgc tgacgaccta cagtatgtgt 1440 tcggaaaacc ttttaccaca ccgctgggat actggcctcg tcacagagat gtctccagct 1500 acatgattgc atactggacc aactttgcca aaactggaga ccctaacagt ggagatctga 1560 aagtccctgt tgcctggcca aaattccaaa acacagacca caagtttttg gagatcaacg 1620 ccagaacgag cactgaatct gtgcaggaga tgttgaggtt gcgctacgtg catttctggg 1680 ccagtgtctt tcccaaactt ccttcactga agtaaacttg tagacccgag gaacaaatgc 1740 aaaataaagt ctgatctaag aaaaaaaaaa aaaaaaaaaa aaaggccgcc tcggccactc 1800 tgcgttgata ccactgctt 1819 <210> 104 <211> 567 <212> DNA <213> Oryzias sp. <400> 104 aaacggtgga gaggcccgaa acttgctccg tgcagtccag gatgggagac accctgcaga 60 tccactacac ggggagactt atggatggaa aggtgatcga cacgtcgctg tcgcgggatc 120 ctcttgttgt cgaactggga aagaggacag ttattcctgg tctggaacaa agcttggttg 180 gtgtgtgtga agggcaaaag atcaaagcta ctattccacc tcacctcgca tatggaaaaa 240 aaggttatcc gcccacaatt ccaggtgatg cagccctaga atttgaagtg gaggtgattt 300 ccttgtcgca gcagacgcca ctgcagaaaa tgatcaacga cgtgtttcct ctagtctgtt 360 tggctctggt gcccactttg ctcggtctgg tgggacttta cctctacaaa aaatccagtg 420 cccaaaaacc aaaacaaaaa gaagccaaag gacaagaaga gcaagaagaa ataaggagaa 480 aaagcaagag gctatttaaa ttaaagtttt taaaacaaaa aaaagaaaaa aggccgcctc 540 ggccactctg cgttgatacc actgctt 567 <210> 105 <211> 590 <212> DNA <213> Oryzias sp. <400> 105 gtctgggata cccggctcca cgctttcact tcccttactg gctggtttac ggaccggccc 60 tgctcctctg gctgctgacc gtgctcctgc ggccttttgt ctccttcaaa ccgacattca 120 cgcccatgag agtggctttg gccggaacct accactacta cagctgcgaa cgggccaaag 180 agcacctggg ctacaaaccg gtggtcaact tgaaggaggg gatcgcgcgc acagttcaga 240 gctaccccca cctcaaacac agcgcttgat tgtagaaaaa aaactgttta tacacaacat 300 cagtgccaaa aacttggcca ggttttgctg aaaggacttc ctgctcacat agctgttatg 360 tcccatttcc accagcagat ggcagcaaat agtccctttt ttgtggattg ataattgcca 420 ttccacctgt tgatacaggt atacaacaga gatgaatccg ccacaatgcc ttattacttt 480 acattcctga catatgtgta aaagcaactg tttaaggtac agttttcaga atttaatgac 540 aaaatatgta aaactaggga tcttaaatca tgattaaaac aggctgtacc 590 <210> 106 <211> 420 <212> DNA <213> Oryzias sp. <400> 106 aagcagtggt atcaacgcag agagggcatc caggccgagt ttgtgggagg agtgctggtg 60 tgcaacaaca tggtggctgt ccgcaggacg gaggccggac gcatcggctt ggaaggctgc 120 ctgtgtgacg actactacaa gatccgagag ctgctgtatc agcagtacgc tgtcgtatag 180 cagcagcagc agcaggaaat aaagctgcag aaaactgcca gacaaaatag aaaaaaaata 240 atactttgta cattttctca ttttttatag tattttgttt ccatgagtca agatggtctc 300 tgtgtaaaca gtctggaggt ctgaacattt tgcaaaattc acccacaatt cctttgtatt 360 tgtatccgtt ttgtttcatt aaacctactt tttcttataa aaaaaagaaa aaaaaaaaaa 420 420 <210> 107 <211> 580 <212> DNA <213> Oryzias sp. <400> 107 aagcagtggt atcaacgcag agtggatctc caacatcgca gtccaccccg gaggtgatca 60 tgtgatctgt gggagttacg actgtaagct gagctggttt gacctggatc tctcgacgaa 120 accgtacaag gttctacgac accacaagag ggcggtgagg agcgtggcct atcacagagg 180 ttacccgctg tttgcctcgg cgtctgacga cggaacggtc atcgtctgtc acggaaccgt 240 ctacaatgat ttgcttcaga acccgctgat cgttcctgtc aaagtcctaa gaggtcacgt 300 gatcacgcac gacctcggag ttctggatgt cacttttcat cccacacaac cttggatttt 360 ctcctcagga gccgatgcaa cgatcagact tttcacctaa aaaaaaatgt taaattgttt 420 tggatctaca ataaagacgg gattgttttt cctttttttt agtcagataa atttcaaatc 480 tttgtctgga atattacagt cttctgtttc tcactacaga aaatagtgtc atttcctagc 540 aacattaagt tgtttgttta ataaattgta atattaactt 580 <210> 108 <211> 525 <212> DNA <213> Oryzias sp. <400> 108 aagcagtggt atcaacgcag agtgtccatt acggccgggg ggaagcccct ccgcagacgg 60 acaggtgaag aagatgacgt cctcctcgcg cctcctcctc ctgctgctcc tctccctcag 120 cgcctccttc agctgctcct ccgccgcgca gagagactcc aaactccgcc tgctgctgca 180 ccggaccccc ctgctgggct ccaagcagga cgtgtctcgc tcctccctgg ccgagctgct 240 cctctcggac ctcctgcaga tggagaacga ggctctggag gaggacgggt tccccctgag 300 cgaaggcgag cccgaagaca tccgggtcga cctggagcgc gccgccgcca gcggcccgct 360 tctcgccccc cgggacagga aggcgggctg caagaacttc ttctggaaga ccttcacgtc 420 ctgctgaggg ccggcctcgg cagaagctcc tccccctgcg gacgctctca ttagtttgtt 480 tagtattttt cttggtttga tctgaacttg atgaaaacta ttttt 525 <210> 109 <211> 270 <212> DNA <213> Oryzias sp. <400> 109 aagcagtggt atcaacgcag agtgagactt ggacatggcc tactctagtc tcaatgctga 60 tgggacttgc tggagaaaga acagccttgc gaagaaaaca agcgtacctg ggaagttcac 120 gtatacgtgt caaagatggg gaagtttgaa tgacttgagt gtggttgatg caaaatatga 180 tgaagtatgc cctgatccan accctcaaaa caaaaggaga tgatgtctac agcgtcacct 240 aagctctatg gccgcagtcc tgacctcagt 270 <210> 110 <211> 1160 <212> DNA <213> Oryzias sp. <400> 110 aagcagtggt atcaacgcag agtggccatt acggccgggg gtaggtcagg agggacgcca 60 tgtgctgttg aggcaggggt gcaggagaag gaccgggctg gaccagcaga acagattcgg 120 ttgaaaattc agcgggacag cggggagacg tattccgtga acgttagcat gtactgcacc 180 agccgctgcc tccggagcgc gctgcgcgcc gccgccagca cgcaccggta ccgacaacag 240 ctccaacatc aggttccgtc cttgtgtgcc ctcagacttc tgaagaccag ctcatcctct 300 cattcagaaa ctattgccac gcctccatta gacggagcac ccaaacagta ctcccccaaa 360 attcagcagc tcgtcaacga cattgccagc ctcaccttac tagaggtgtc ggatctgaat 420 gagcttctga agaaaactct gaacattcag gatgttggaa tgatgccgat gggggcaatg 480 gcagcaggtg ccatgccagc aacgccggct gcagaggagg aggaggcacc ggccaaaaaa 540 gagaagactc acttcacagt caaattgaca gaactgaaag cagcagataa agtaaagtta 600 ataaaagaag tgaaaaattg catccaaggc ttgaacctgg tccaagctaa gaagcttgta 660 gaatctcttc cacaagaaat ccgggccaac gtatccaaag aagaagctga gaagctgaaa 720 gcagccctgg aggctgcagg cggcactgtg gtcttggagt aaatcagtgc atttctacat 780 ctgcctctta cattgccatt tctctggcca cgcacttcgt ttttcattat tttataactt 840 tttttttttc ctgggcgaag acgtggacac tgcaaaagaa gcaaatgcct tcatttactc 900 gaagcgtcca tagcatcagg tggggtgctg cagattgaac ttttccaggg acacggcctg 960 cacaaaccac ctccttgcag tttgtttgtg tgctcccgtt tgtctgaaac tcgtgtagca 1020 aacgctacca aaactctggc ccccggtgcc aggcggtatt tctgtgtgtt tgtcaagccc 1080 ttcataaagg gacggtttcg ctgtgaaagt aggaatactg gaacagcagg aactgtattt 1140 gactctgaaa tgagtagtta 1160 <210> 111 <211> 747 <212> DNA <213> Oryzias sp. <400> 111 tccgaactgg aggccctgga gtgtcacttc acctgggtca aggatgtcac tagatcacag 60 ttgttggaca tgaaggtcaa gcttgaggac attgggacac atgagggaaa tttttggttg 120 ggctttattt acaacatgtg ggggtttgtc ctctacaagc tggactctag tgaagaagcc 180 cttcagttcc tcaggaaggc cacagaaacc ttaaagcgct tgagggagga agatgagggt 240 ccctggttgg tggtgaacta tgggaatctg gcctggctgc accatcatct tggagaagag 300 gcaatgagtc aggactacct gtcaaagatt gagctctcaa aagtaaattc ccatctccat 360 cccaggacga gctccatcca gagatctacg ccgagaaagc ctggatctga tgaagttagg 420 taaagagaag aagcagctgg ctgcagatta ctttcagata gccttcaaca tgcagccaga 480 catggtgcag tggcaatcca gccgtgtcat tgcactggcc agtgctgcta agcactggga 540 cacagacctg agtgatgact tcttgcagga aataagaaca gcccgagagc aggatccaga 600 gaatctgtac gtggctgctg tggagcttaa agtaagaaac agaaaaggag aatcagtgaa 660 agatgaggcg gagacgctgg gccagaagat cctcctgcag cctgtcagca gctacagtgg 720 tctgaagcct ctcctgaggc tgcacag 747 <210> 112 <211> 2103 <212> DNA <213> Oryzias sp. <400> 112 aagcagtggt atcaacgcag agtggccatt acggccgggg atgttctgtg agaaaggaca 60 accattgaca gctgagaaca ggagaaatgt atcaagcatg tggaggagtt gtattcttta 120 gtctatgcct cattttcttg gtgccatcat gcagtggagg aaacattctg gtgtaccctg 180 ttgatggaag ccattggata aacatgaaga ttcttctaga agaacttcat gccaggggtc 240 acaacatcac tgtgatcagg gcttccagca gctggtacat taaagagaag tctcctctct 300 actcatccat caatcttgaa atggaagagg cctttgaaga tttctttgat attttcgtag 360 ctgagcaaat taaggtgcag cgagaaggtg cttcaatgtt gacgttcctc aaactcaaca 420 gagactttct ttcaatgctc acccaggctc atgtattgtg gtgtgatgtt acgaaagaca 480 tctttgaaaa taagactctg gcaaaacaaa taattgattc aaagtatgat ttggttctga 540 ctgatccagc aataggtgga ggtcttgtct tagccaaata ttttaattta cctttggtac 600 tcaatgtacg ttggatccca cttggagaag gccatcttgt tctggctcct tctccacttt 660 cttatatccc attaccagga tcaggcttaa ccgatcaaat gaactttatt cagaggctga 720 aaaacatatt tttttacggc atctcaaagt ttcaggaaat tgtcttggtg ggacccattt 780 atgatgacat ctgcagtaaa tacatagagg ggggatgtag tgtcgcctct ctgcttcagc 840 aagcagacat ttggcttttc agatcagatt ttgtgtttga cttccctcga cccaccatgc 900 caaacgtggt ctacatcgga ggattccagt gcaaaccagc tgaacctctg ccagcggatc 960 tggagaagtt tgttcaaagt tctggagaac atggagtgat aatcatgact ttgggaacac 1020 tagtcaatgc tctgccaaaa gacattgcag atgagattgc catggtcttt tccaagatgc 1080 cccagaaggt gatatggaaa cacaaagggg accgtccctc tacactgggc agtaacactc 1140 tcctggtgga ctggatgcca cagaaagacc tccttggtca tccacaggtt aaagtctttg 1200 tagctcatgg aggaaccaac ggagtccaag aggccattta ccacggagtg ccagtgctgg 1260 gcatcccact gttctttgat cagtacgaca acctattacg tttacaggaa agaggagctg 1320 gaaaaatcat tcaacttgcc gacttgaata gccacacctt tgaagaaggt cttcaggaac 1380 tgctccataa tactagctac aggcagaaca ttcaaagact ttctcgtttg cacaaagatc 1440 aaccaatgtc acccatggat caggctattt tctggataga gtatgtgata cggcacaaag 1500 gggctcgcca tctgatttca gaggcttaca agatgccttg gtactcatat cacagtttag 1560 atgttctcct tctgctgctg gctgtactaa cggtgctgct ttactccatt tatgctgttt 1620 tcagatgtct atgctgcaga aggaaaagaa agacaaaaac taaacaaaac tgaagttgtt 1680 agatttgttt gaaaaaaaat gtgtttggaa tttaaatttt atgcatgtat ttaataaaac 1740 ataaaagatt ttttttttta ttatgttgca attttgaaac caagggattt ggatgattgg 1800 ctcataaatt tacatggtaa cttattacac aaatctagtc agaggttctc tctgaactat 1860 ttttttaaaa ctttttcttt cattgcaatg tatgtacttg ttcttgtttt aattttccaa 1920 tcacaatccc atcgttaact tgttgaatgt aacactttct tattgcaatt accacttaaa 1980 ttttaccagc tctcaaatac ttttgctttc tcaacatttt gagttctgct tctgatctca 2040 attttctttg caaattttat ctttttaaat tagtatatat cattaaagta cttttttaaa 2100 tct 2103 <210> 113 <211> 993 <212> DNA <213> Oryzias sp. <400> 113 aagcagtggt atcaacgcag agtggccatt acggccgggg acctgcaagc gctgcacgag 60 gctgccagac acagaggtcc ttcactgtaa cgtaaagaaa aatcagaaat gggcgtttct 120 tcggcacaag ggagtcttca tccgtactgg ccgcgggacc ttctgattcc caactatgtg 180 gctaatgacc gctccatgtc cgagattctg gctttccttt tctcggtgtc gggggtgttt 240 ctgctcgcca cctggctgat taccggggcc gcggacaggt ttgggatctg gaggcggctg 300 gctatttgtt ggtttgcagt gtgcggtttc atccatggtg tcattgaagg ctggttctca 360 ctttattacg acattctacc tgcagaccag agtttcctgt cacagctgtg gaaagagtat 420 tcaaaaggag acagcagata tgtaatagct gataacttta ctgtctgcat ggagactgtg 480 actgcttgtt tatggggtcc cttcagcttt tgggctgtat actcttttct atcacataaa 540 ccctacagat ttattctgca gctcatcatt tcattagggc agctgtatgg agccgtgctt 600 tacttcttca cagaacacag agacggctat gcccacagcg agttgggaca tcccatctac 660 ttctggttct actttgtgtt catgaatgtt ctatggattg tcataccact aatgcttatc 720 gtggatgcat ggagacagct atcagctgcc cagaaacaca cagatggcat caggacccac 780 aagagtaaaa agaagtaatc tcaggatacc aagggactga aagacactct ccttcttaac 840 aatctttatt tcaacaacca gacattcctg atgctgatta aagcagaaag actacaatga 900 ttgaatgtca aattattgta ggcaaaactg aaattttctt gttttgtact tttttaagaa 960 atctgcttct gacaattaaa atatatatat ata 993 <210> 114 <211> 2171 <212> DNA <213> Oryzias sp. <400> 114 aagcagtggt atcaacgcag agtggccatt acggccgggg ctgactctgc tgtgagggca 60 tcgggccaac tccgagaggt cctcccactc tcctccaacc aggcaggcac ccggctgcgc 120 acggagctcc tcaccgccac agtctcaccg cctcctccgg acgcagcaga cagcgcgcgg 180 ctcctccacc tcctcagcgc tccatcagct ggctcctcaa atggtcaaaa atggactctt 240 taaatcataa cctcaacact tactttgaat catggctcgg ccctagagat aagcgggtgc 300 agcaaatgct tttgctcgac aactacctgc caacctttgc acttaccgtc atgtaccttc 360 tgatcgtgtg gttggggccc aagtacatga gacaccggca gccgtattcc tgcagaggtc 420 tcatgttgct gtacaatctg ggcgttactg tcttgtcctt ctacatgtgc tatcagcttg 480 ttagcacttt ttggagcggt ggctacaact tttactgcca gaacacatac agcaaaccag 540 aagcagattt aaagatcgta aatgccctgt ggtggtacta cttttccaaa ctcattgagt 600 ttgcagacac tttcatcttc atcctacgaa agaacaatca tcagatcaca tttcttcacg 660 tctaccacca cgctagcatg ctgaatattt ggtggtttgt catgaactgg attccctgtg 720 gtcactcata ctttggtgcc tccctcaaca gcttcgtgca cgttgtgatg tattcttact 780 atggtctctc atccatccct gctctccggc cttatctgtg gtggaagaaa tacatcacgc 840 agctccagct gatccagttc gttttaacca tatgccagac tgcctgcgct gctatatggc 900 catgcggctt ccccatcagg tggctgtatt tccagatcag ctacatgggg acgttcgtcc 960 tacttttctc aaatttctac attcagacat acaagaagca gagtgcctca cggcagaagg 1020 agttcaagaa tggttcttct atgtcgacga acggtcacgc aaacgggacg ccgctggtgg 1080 agcgcgccgc atccaagaag ctgaggacgg attgacgttg gagaaagcct cactcaagat 1140 tagctgtagc gtgttagccg atgctgctag ggggcgtagg cgtcctctct tctagaatag 1200 tcaagaacta atttacaata aaacaagcca tagccacaca tacaatacaa aggagacaaa 1260 aacacaaata ccacttattt ggtttaatca tgttaccctt ataattatag aagaatacta 1320 atgcactagt gttgacacag caataaactt tctttgcctt ttatgctaat ttatttcaga 1380 acagaatgtt ttttttcact gccagagcaa aaaaaanaaa aaaaaaacta cacatctaca 1440 ctcattcagt gatgctttac acaactaaag tccaaagatg aacaatcaga tgtgttgaca 1500 gataaatgtt ttatgacaga tcagtgtgag aatgaaggct atgatctttg aacgtttatg 1560 ccactccggc cagcctacct gtgtgtgtgt ctacatattc cagaatgatt gttctcagta 1620 atactttcaa tcatcaatct ctacaaataa agagcttcaa cagctttttg caggtgtgtg 1680 aaaggacaac tgttgtattt gtgtaaacat gtcaaagaaa ctcagttttg tcagtattac 1740 agaaaatctg tgtctcactt tgtccttttg gcaccttatt taaaatggtc atgtctgttt 1800 tgttgtggag aatcacagag tttgaaaata caactatgca gtacagttta ttgttaaata 1860 ttacagtaga attacagttg gatttgctgc accatttagg acgtaatttc tttcaatttc 1920 tgcataaaga tgacttaatg tatgtttaat aaaggtaatg gtataagttt tatgtatgtg 1980 tctggcaagt ttatttcaag ttaaggtcac aaacatcaat caacagtttt acaactctca 2040 atttctgctg aaattcagtt tgttgtgaca atttaacatg gtaatttaca ttttttgagg 2100 gaagttgtgt tttatttgct atttacattt tttgaaacta caatttgcat taaaatttat 2160 ttaatataac t 2171 <210> 115 <211> 545 <212> DNA <213> Oryzias sp. <400> 115 caaggtcttt gctgttgcca tctcgccaga ccaggaggac acccgactct cactcattgc 60 cacaaaccag aactatcgac agaacttcac agctgcagac aacatcagat ccaccaagat 120 gcaaaccatt cggtccatca ttgacatcat taccaacgaa acaaaggatg tgtgctgctc 180 ctttgactgc aacccttccg gaggagccaa aggaccacct ggagatgccg gtggaatggg 240 agagatgggt agacctggga tgccaggaga gaaaggagac ataggcaatg tgggcagcgt 300 tggagatcct ggacctgttg gctacagtgg gatgaaggga gatcgaggtc caagaggagg 360 aaagggtgaa agaggagcca aaggctttaa ggggacaaag gtcaaagggg gttagatggg 420 actgatggac ggaagggtga ggctggtttc cctggacttc ccggatgtaa aggttctcca 480 ggaggcgatg gccagcaagg ggagcctgga ccaaagggag atgctggtcc ttatggcagg 540 aaagg 545 <210> 116 <211> 405 <212> DNA <213> Oryzias sp. <400> 116 aagcagtggt atcaacgcag agtgtcctct tcttctttag cgtcatcatc atcttcatcc 60 tcctcctcct cctcagagga agcctcaaac caaagccctc tcaacctgag cccagtggac 120 tgcctgctgt gtccggtgtg cggggagagt ttcaccagca gggccggcca ggagaggcac 180 ctgcgcctca tgcactcctc ccagatctac ccgtgcaagt actgccccgc caccctctac 240 agctcaccgg gcctcaccag gcacataaac aagtgccacc cctcggagaa caggcaggtg 300 atcctgctgc agatgccggt gcgccccgcc tgctgaagac aacacagaac tcgtgccttt 360 gatggatgga gggaggaagg aaggagaaga aaaaaagaag aatgg 405 <210> 117 <211> 961 <212> DNA <213> Oryzias sp. <400> 117 aagcagtggt atcaacgcag agtggccatt acggccgggg gttttgagca acgttagaaa 60 aagagctcca ggtgagaatt tggttgcagc tgaagaagat gtacgagcct ctggaagaca 120 gtgttgagat tacgttccct gtgaagtttc tgggccgggt ggaggtggtt cgttctgatg 180 gacttcagat cctggaagag gcggcttaca gtctaaagac cccagataag tattcatctg 240 agaaggtgac gaagaacact agagtcctca tcttcctatc tctgagtggg attgacatct 300 tggagcataa aaccaagttt ctgctgtacc agtgttctct ctccactgtc tccttctgcg 360 ccgtcctgcc atcttcaccc aaagttttcg gttttgtggc caaacatcct gcctcagata 420 tctaccactg ctacttgttt cagagcaaag cctatgccca cgtcctggtg tccgccatcg 480 gagatgtctt cagagcgtcg aaaaatcagg aaagcgtcag aggagggcga gatctgatcg 540 tggaggccct caggcacaag aataaaatac ttcagaagga gaacgatgag ctgaagagga 600 agcttgcagc cctgcagaaa taaaaagatg gctcatacgc aatcaatttt tcaacttcct 660 gtttcaagtt cgttccagaa aacgcttcaa aaacctgcaa gaaacatgaa aaaaaaggtt 720 tttcctaaag ctccaaatct ttacatttaa tttaaataat ttaatgattt tttttcttat 780 ccagcacttt actgttcatg atactgaaac cttttttccc aaagtgtaaa gataaatgtg 840 ttgcttttga ctacatcctg tttgctctgt acattcattg ttcggtgctt tagaaaaata 900 aatccttttt gccaacctgg gatataaata caacaaaaat gttcaaacag taaaataaaa 960 t 961 <210> 118 <211> 538 <212> DNA <213> Oryzias sp. <400> 118 aagcagtggt atcaacgcag agtgccatta cggccgggga acacaaactt ttgtttaaag 60 ctcacgatgg agcctgccat cgatgtcctg atgtctaagt ttaaggtcta cgctggaatt 120 gatggttcat ccgtgactct gagcagggat gagtttggaa aactggtgaa atcagagctg 180 cccaactttc tgcagaattc tggagaccct gccgccatcg atgagctcat gagatcattg 240 gacaagaaca acgatggaga gctgagcttc atggagttct ggcagctgat tggacacctt 300 gccagcaagc atgggggctt agaccaatag cgtctcaaca gacagcttcc atgcgttcca 360 gttctgtcag cgtgccatcc tcgcttctgc ttttctttca cgccctgtct gaccaaagtg 420 aacccggtgc tgccacagga gctggtcgtg cgcagctgcg ctctgattta cgcgcacatc 480 tagaatcatg gaacggagtg ggaatgcatt gtcttcatga gcagtttctg gctcctct 538 <210> 119 <211> 431 <212> DNA <213> Oryzias sp. <400> 119 aagcagtggt atcaacgcag agtgccatta cggccggggg tctgagtctc cagctcgagg 60 agcttcagtg tggcagcttg acccaacctg gaccgatacg ttctgcaagc atggtggata 120 ctttctgcgc tacgtggaag ctggtggaca gccagaactt tgatgaatac atgaaggcac 180 ttggtgtcgg ttttgccaca agacaagtgg gcaacgtcac caaacccaca atagtgatca 240 gccaggatgg agataaggtg gtggtgaaga cccagagtac cttcagaaac acggagctct 300 ctgccaaact gggagaggag tttgacgaga cgacacctga tgaccgtcac gttaaatcca 360 ctttcaccat ggacggagac aaatttgtgc acacgcagaa gtgggatggc aaggagacca 420 agtttgtgag a 431 <210> 120 <211> 649 <212> DNA <213> Oryzias sp. <400> 120 aagcagtggt atcaacgcag agtggtggtg tccgacggga tcggcttacc gaacgcgctg 60 gcctacgact actccactga tcaggtctgc tgggccgatg caggtacgaa gcgtttagag 120 tgcgtatctc caaacggctc cggcagcaga gtgatccatc ccaacctgaa ctacccgttc 180 agcatggtct actacagaga ccacttctac tacacagact ggaggaggga cggcgtgatc 240 gcagtgaaca gagacagcag caagattacg gaggagtacc tacctgacca gcgctcacac 300 ctgtacggca tcgctgtagc aaccaaccac tgtctaccag ggagccacta accaccagcc 360 ggcagctatg gggggggcct atcggtgcca cagaggcagg agacggtcca ggaggagccg 420 ctcccctccc cccgctctga agatgtaacc caaaagttct gtacatttgt tttatacctc 480 atttctaaag tttttactgt atttttttta tattttgtat cgattaaaag ccacaatcac 540 ctaaagattg ttcaactctg aggccaaacg tcaccagaaa cggagaagaa ataaacagag 600 aaactgcctg aactcacctc tgcactaact tctcaccttt tttttgttg 649 <210> 121 <211> 2145 <212> DNA <213> Oryzias sp. <400> 121 aagcagtggt atcaacgcag agtggccatt acggccgggg agctggagtc caatcaggca 60 cgtggggcaa aattcttcaa catctcagct aagaagtggc actgagcatg gaacaacaca 120 tgaatggcca gagccctgag acgggcttaa ggaatctcag caaattctgt caggctgtca 180 gagatggtct tactctggag gaatcagctg tggaaatgaa agcagctgag tgcttcatcc 240 acgaagccat ggaagtgatt attgatgaag cagtaaaaaa ggccactgat gttcaagaaa 300 aggtatgtga gtggcactcc cctgaacagc taaaagagct gctggacttg gaactgaaag 360 atggagggga atctgactct aaaattctgc agcgctgcaa aaatgccatc agatacagcg 420 tcaagaccag tcatcctcgt ttttttaacc aactgtttgc agggctggag ccttactcta 480 tggtggcaag cttcatcatc gaagcagtca aaaccagtct gtacacatat gaggtggccc 540 cagtctttac cttgatggag gatgctgtgt tgaaaaagat gatggagttg atcggctggg 600 atgagggagg agatggcatc tttaatccag gtggatcaat atccaatatg tatgctgtga 660 gtttagccag gtatcgattc tgtccagaca ttaaagaggt tgggttctat gatgctccac 720 gactggtcat gtttacatca caggagggac attattcaat ttccaaagct gctgctttgc 780 tgggatttgg cacaaaaaat gtttatgtgg ttccatccga taacagagga aagatgattc 840 ctgcggctct ggaagagaaa atagagttgg cacgtagtga aggcgcagtc cccctcttgg 900 tgaatgccac cgcagggacc acagtgttcg gagcctttga tcctgttgac aagatcgcag 960 acatctgtga gcggcacagt ctgtggctgc atgtggatgc atgttggggg ggtgcagctc 1020 tgctttctaa tgagcataaa cacctgctaa aaggcattac cagagctgat tctgtagctt 1080 ggaacccaca caaaatgctg atggcttgtc tgcagtgttc agcattcctg gtcaaagata 1140 aaatgggtct cctccaacgc tgccactctg caaaggcatc atacctcttt cagcaagaca 1200 agttctacga tgtgagctat gacacagggg acaagtcagt ccagtgcagc aggaaacctg 1260 atgcttttaa gttctggctc atgtggaagg ctttgggatc tacagagctt gagcgtaggg 1320 ttgacaaagc tctggccatg gccagatatc ttgctcaaga agtcaagaaa agagagggat 1380 ttcgtttgtt aatggaacct gaatatgcaa atgtttgctt ctggtacttt ccaccggcgt 1440 tgagagacat tcctgatggc cctgaactct ggagaaagct acatactgta gcccctgtgg 1500 tcaaggagcg gatgatgaag acaggcagca tgatgattgg ttaccagacc catggggaca 1560 agccgaactt cttcaggatg gtggtcatca atccccaagt cagcaaaaaa gacctggact 1620 tcgtcttgaa tgagatccat aacctgggaa aggacttaag atgaaacatg aacacttttc 1680 agctctgctg acagcagcag aagattacag ttaatttctc tacaatacaa ttcatagtga 1740 ttattgtgtg taaaagtttg tttgctaagg caatactcat ttggaagtct gtcttttgta 1800 tcctatactg cccttgattt ctgacctggt ttgttttgag cattgtttgg gggtgctttt 1860 ctttgtgcct agtgctaaca ctttcaatct ttggacacca gttttgggga tttttgtctt 1920 ttgctgcccc ttcctcactg tttttgtttg catgtctatg gactccggtc gcattttctt 1980 gtctactgcc ttattttaat gggtggtgtt actcaccctt cccctgcctc agtttttttg 2040 tgggtggggg tattgtgtcc tgttgctaca gttagaagac aataaaaaca tgttcatcct 2100 agtttaataa aaaaaaaaaa aggaccgcct cggccactct gcgtt 2145 <210> 122 <211> 1333 <212> DNA <213> Oryzias sp. <400> 122 aagcagtggt atcaacgcag agtggccatt acggccgggg gcttggcagc tggtgagagc 60 aaggacggtg gtgtcgacgc tagaagtttc tttgcagaca ataaccatcc aattcggaat 120 ctaaaacctc ggaataagga aactggacac gtttgaattc gcttaaatta ctcttcagca 180 gctgttgaat actgcaacca tgccagaagt ctttgcaccc gcagacatgg gcagctcacc 240 tccatccccc gtggacagca gtccgaggag actgtcctgg ggcaaactgg tccacaggtt 300 gacagatttc agcacaaaca gcagcagtat cacgtcaggg tccaacaatg gcagcaggag 360 tgacctctcg gaatctgggt ccgatacctc cagtgacctg tcaccgtaca atgatgacct 420 cttcttcgat ccgatggagg aaaccatcct gaaagaagtg gtggacctca tcgcatccag 480 ccttagagaa gccaaagact cggactgtgc tctgaggtgc gccaagctcc tcattcccga 540 gaaactgctg gagcacatcg gacgggagct ccttcatctg gctgccagcg agccctgcgg 600 attgaggggg gctctcattg acctttgtgt ggagcaagga gatgtgtgtg agagcatggc 660 tcagctatcg gtggacccct gcctcgtccc cacttttcag ctgactttgg tgttgaggct 720 tgagtccggt ggcttgtggc caaaaattca aggacttttt agctccagat ctacttccac 780 tccagcagtc cgacaagcac ttaaactcag cactggtttc cgcgtgatca aaaagaaact 840 gtactgttct gaagacctgc tcattgagga atgttaagaa atgaaagact cgtttttttt 900 ttaatataat gcgagcttac aatgaaagct caaagaaggg ccttttaaga ttatgcctac 960 agaagtataa agaaggactt tgttctgatg tagcctttgg ttcctgctgt aagtttctgg 1020 tacttggatg tcttcatgca gcacttgctc aaaacttaag tttaaaaaat gtaaccatat 1080 aatatctggt acatctgcat ttgaaactga atttgaattt taaatgcacc gacgtttcta 1140 ctgaaacatt gaaaaaggca gctagctgta ttgtgttacg cattgaagac aactgaattg 1200 tacggcaact catgacatgt actccaattc ctcagtttgg gaacatttaa gctaaagctt 1260 gcacatgtgt aagcctccac tttgatatca cgtctaagtt tttttttttt ttttgttttt 1320 ttttttcttt tac 1333 <210> 123 <211> 2120 <212> DNA <213> Oryzias sp. <400> 123 aagcagtggt atcaacgcag agtggccatt acggccgggg agtcagagga tggtttatat 60 gatgcttgcc aggctttttg agcgaatctg cattgaaaag actctgctga tgtgaaagga 120 gccaagaatc tggatttggt gtgaaacttt cagccagacc gttccgttac caagtttaag 180 tcagcagcag cagcagccga gaggatggga ggtggaggcc agctgtctgg gagcgtggag 240 cccggcagtg ggagagattc aggtgttttt acctgggagg aggtgcagaa gcactgcacc 300 aggaccgatc agtggctggt gatcaatcga aaggtttata acatcactca gtgggtcaaa 360 agacacccag gagggtctcg tgtcattagc cactatgctg gagaagatgc cacggaggca 420 ttcactgctt ttcaccctga tctgaggttt gttcagaagt ttctgaagcc tctgctgatt 480 ggggagttgg ccccaacaga gcccagtcaa gacagaaaca agaatgcctc catcatacag 540 gattttgaga acttaagagt tcaggcagag aagaatgata tgtttaaaac taagcctctg 600 ttcttctgct tccatctggg tcacatctta ctgctggagg ctcttgcgtg gctcctcgtc 660 tggttatgtg ggactggttg gacgatgaca tttttgtgct caataatttt ggcaactgct 720 cagtcacagg ccggatggtt gcagcacgac tttggccacc tctctgtttt tagaaagtcc 780 aaatggaatc atctggtgca caagtttgtc atcgggcatt taaagggagc ttctgccaac 840 tggtggaatc accgacattt tcagcatcac gcaaagccca acatcttcag gaaggaccct 900 gatgtcaaca tgctggacat ctttgtactc ggaaacactc aaccagtgga gtacggagtt 960 aaaaagatta aacggatgcc ctacaaccac caacaccagt attttttctt ttgggtccac 1020 ctctgctcat tccagtttac ttccacatgc agataatgaa gactatgatt caccatcatc 1080 actgggtgga tctggtttgg tctttatcct actaccttcg ctttttcttt agctacatcc 1140 ccctgtatgg tgtgtttggc tcttttgccc tcctcacctt tgtcaggttt ttggagagtc 1200 actggtttgt gtgggtgact cagatgaacc atctaccaat ggagattgag aatgagaagc 1260 accaggactg ggtgacgatg cagttgcagt ccacatgtaa cattgagcag tccctcttca 1320 atgactggtt cagtggacat ctcaattttc aaattgagca ccatctgttt ccaacgatgc 1380 cccgccacaa ctaccacctt attgccccac aagttcgtgt tctttgtaag aaatatggga 1440 tcccttacca ggtcaaatct ttatggcaag ggattgtgga tgttgtcagg tctctgagga 1500 cttctggaga gctgtggctt gatgcatacc tgcacaaata aaccaaacaa caataaaata 1560 aaaaatgtgt ttgcaataaa ataaatattg tacctttgtg gatccactta cacagtttgg 1620 attcataatt tgttttcagt gaagtttaaa ttataaagtt tttagtcttg ttatcggact 1680 ggaaaggggt ttttgattag attcttctga acaaatgttg gtaatataaa taaaacttta 1740 aaatctgtaa gtcacttgaa attatttaca tctggtgatt ttatatgagc aatgaatctg 1800 aaataagtta aattattttg acacgaaccg taacattaat atgaaactga atcatgtaaa 1860 ttctttataa cttgtgaaaa taagcaactt ttacactttt acacaacaca ataaaaacct 1920 gaaatgaaat tgttcattat tttgtaattt cttaagttta aatgatcact gttctgttct 1980 ttttaaggat ctcctacatg tatagtacat gcattcttgt cagatttttg ctgtaattaa 2040 aaactatttt gagacctgca taaaaaaaaa aaaaaaaaaa aaaaggccgc ctcggccact 2100 ctgcgttgat accactgctt 2120 <110> Korea Institute of Ocean Science & Technology <120> Nonylphenol responsive genes in Oryzias javanicus and the method          for diagnosing aquatic environment pollution using the same <160> 123 <170> KoPatentin 3.0 <210> 1 <211> 232 <212> DNA <213> Oryzias sp. <400> 1 cgggaccaga tgaatgccat ggcaaaacga aacattctgg agcgctgctg ttacatgccc 60 tgcaccatct acgacctggc gaactattgt tcctgaagag ccggcgcaga caccaatcgc 120 tgttagatta gcttacagag cagtattcaa ttagctgttc aagtgtcagc aacagtagtt 180 cttattgcta gctatccgtt tttaaagaat gtgctgataa attattcaaa at 232 <210> 2 <211> 325 <212> DNA <213> Oryzias sp. <400> 2 tttccaatgt tggtcacgac cttcttgaat tcaagggttt tgtctgcctc aaacggcccc 60 aaagtggtcc attcctgggt ctcaagaagt gcagcagaga aggccacctg tctcgctgat 120 acatccctct ggagctcctc cactttggtt gttagctgtt gcactttggt gttcagctcc 180 tgcagtgtgg ggtcctgagc ccccaccaaa ccacaggcaa gagccaccag aaggatccag 240 ctggacatca tctttgctaa gagagaaccg ggcttaaagc aggctccccg gccgtaatgg 300 ccactctgcg ttgataccac tgctt 325 <210> 3 <211> 383 <212> DNA <213> Oryzias sp. <400> 3 aagcagtggt atcaacgcag agtggccatt acggccgggg gtgtctcgac gcaggaagca 60 gccatgaagt tcttcattct cctggccctt tgtgctgcag catatgctgc tcctcttgag 120 gatgacaaga tcgttggggg ttacgagtgc aggaagaact ctgtgcccta ccaggtgtct 180 ctgaatgtgg gctaccactt ctgtggaggc tccctgatct ccagcacctg ggtggtgtcc 240 gctgctcact gctaccagtc tcgtatccag gtgcgtctcg gtgagcacaa cattgctgtg 300 aacgagggaa ccgagcagtt catcgactcc gccaagatca tccgtcaccc cagctacagc 360 agctggaacc tggacaacga cat 383 <210> 4 <211> 155 <212> DNA <213> Oryzias sp. <400> 4 aagcagtggt atcaacgcag agtggccatt acggccgggg tcatcaacag gatcactcag 60 cagccatgag gtctctggtc ttcgttctgc tcattggagc cgccttcgcc cttgatgacg 120 acaagatcgt cggagggtat gagtgcaccc cctac 155 <210> 5 <211> 685 <212> DNA <213> Oryzias sp. <400> 5 aagcagtggt atcaacgcag agtgccatta cggccgggga gagacaacaa gagaaggaac 60 taagtgccac ttgggaagaa gaaaaacaga aggttacaac catgaaaagc ctccagtcgc 120 tggccactat cctcctcctc ctcggctttt tccacaacag ctggcaggtt cctctactcc 180 agaacgagga cagctccagt tttacagcag acgacacgct agctgatgag ctgcgggagc 240 tgtccagcat gaagagacac tcggagggaa ccttctccaa cgactacagc aaatatctgg 300 aggacaggaa ggcgcaggac ttcgttcggt ggctgatgaa caacaaaagg agcggtgatg 360 caggcaagcg ccatgcagat ggtaccttca ccagcgacgt gagcgcctac ctcaaggagc 420 aggcaatcaa agactttgtt gccaagctga aggccggtca aatcccaaga gagtagggtt 480 gtccttcgta tctcctcctc ctcctcctcc tgcagtgaaa acgacccgtg tctgatcctg 540 gcgaccgacg agccaatcat ctgcttcttg ttcttcttct ggcgcttttt ccctgatttt 600 cccaaaataa aaatcaccta aacttttaaa aaaaaaaaaaaaaaaaaaag gccgcctcgg 660 ccactctgcg ttgataccac tgctt 685 <210> 6 <211> 470 <212> DNA <213> Oryzias sp. <400> 6 ggtgcaggag gagcctccat cctcaccttc tgggatccca gactttacaa gatggctgtg 60 ggatacatgc tggctcatcc ttacggattc accagagtca tgtctagcta ccgctgggac 120 cgcaactttg tgaacggaaa ggatcagaat gactggattg gcccccccag caacggagac 180 ggatccacca agccagttcc catcaaccct gaccagacgt gtggagatgg atgggtgtgt 240 gagcacagat ggcgtcagat tacgaacatg gtccaattcc gtaatgtggt cagtggacag 300 cctcatgcca actggtggga caacggcaac aatcaggtcg cctttggccg cggaaaccgt 360 ggattcatcg tgttcaacaa tgacgactgg gctctggatg tgacgctgaa cactggcctt 420 cctggcggca cctactgtga cgtcatctct ggcaataaga acggaggcag 470 <210> 7 <211> 110 <212> DNA <213> Oryzias sp. <400> 7 ggatacatgc tggctcatcc ttacggattc accagagtca tgtctagcta ccgctgggac 60 cgcaactttg tgaacggaaa ggatcagaat gactggattg gcccccccag 110 <210> 8 <211> 397 <212> DNA <213> Oryzias sp. <400> 8 aagcagtggt atcaacgcag agtgccatta cggccgggaa aaaaattctc agattttctg 60 gagagacgac agagttaacg gacatgaagc ctgtcattct tacggttctg gttctgctgc 120 tcgtcagtca gaatgaggct ttgaagtgtt actgtgaaaa cacggtggtt tgtgacggac 180 ccgtccacga gtgtaaacct gaagaaacgg tctgtctggc gacggccctt cttcaagtcg 240 gtcgatactt caggaggtgt gcagaaccca gcgcatgctc caacctgaat gtacctggtg 300 ttgcctattc cacttgctgc aggaccgact tgtgcaactg atgctctgga gccgattaaa 360 actgctttga attcctcaat aaaaatctat tttacac 397 <210> 9 <211> 130 <212> DNA <213> Oryzias sp. <400> 9 aagcagtggt atcaacgcag agtggccatt acggccgggg agtgacggtc acaatgaagt 60 tcctgatctt ggctttgttt gttgctggtg cctatggttg tggcctgccc acataccccc 120 ctgttgttac 130 <210> 10 <211> 635 <212> DNA <213> Oryzias sp. <400> 10 aagcagtggt atcaacgcag agtggccatt acggccgggg gagaccttcc ctgcaccacc 60 tgcagctgag acccagggag cagaattaac aagcaaattg aaacagactc caaccagcgt 120 tggagatgct ttcaataata tgttgttcat taagtcgggc tctttgttgt ttttgttttt 180 ttgcaactga atctctgctc tctctcgctt gctgccagct gactgacgtt tagtgggaga 240 agaaaaggaa aggatgtcca acaacatggc caagattgca gatgctagaa agactgtgga 300 gcaactgaaa cttgaggtca acatagagag gatgatggtg tcaaaagctg cagccgagct 360 gatgtccttc tgtgaatctc acgccaaaga ggaccctctg gtgacaccgg taccttcatc 420 agaaaaccct ttacagagaa aagaaagctt ttctgtgcca ttctgtgacc ccctacgacc 480 ctccaaagta gacatgtagc acaaaaatta acatttttat ttgtttttag agtcaatgca 540 gctcttttgc attgatctat gagcatgttt agaaaaaaaa aanaaaaaaa aaaaaaaaag 600 gccgcctcgg ccactctgcg ttgataccac tgctt 635 <210> 11 <211> 247 <212> DNA <213> Oryzias sp. <400> 11 tggatataac ccagagacag gcatcttcac ggctccagtc aaagggctgt actacttcag 60 ggtcactggg attgctggga gcaatgggga attaaacgca ggactcaaaa agaacggaca 120 gaacatgttt gcaatctacc agaaggcagg aacacaggct ggcacctcaa acgggatggc 180 tctggaactg gagcagggtg accgggtcta cgtccagatt tggccaaact tgaccgtggc 240 tgaccag 247 <210> 12 <211> 697 <212> DNA <213> Oryzias sp. <400> 12 aagcagtggt atcaacgcag agtggcatta cggccgggga tacacctgcc aacatgaagg 60 ttatgctgat ctccatggtg ctcctgctgc tgtgcagcgc tcaagtgctc acactcaaat 120 gctacacctg cgatggggag aatgacaaaa tctgtaagac ggagaccgtc tgcccccctc 180 ttaaagatta ttgcaggacc taccagaagg gtgatgtgtt ttcccgaagc tgtgagagcc 240 tctgcgcaga ggactacttc accacctgct gcaccaccga tctctgctga tctaaccttt 300 taaaatcaag ataaatcaat gattatctct tccatttagt tcgtcttggg tgtggaagcc 360 aaatctggca atctttatct tttttttcca cctttgatca aaatgaaaca cagaaagtgg 420 acagttgagt ccttttcatc gtcccacagc ctggcttttt atccctcagg ccgggtgggt 480 gtttcaaaag cgggacaccc actcgcttac actcttttaa tccgctcctg atgtcactta 540 ttgggtcaca gctgcagtct tgtgctatgt gggtcactgt ctataagttg tactcctgct 600 ctcatctgtg ttaaactcat taaagaaaag tggaattaat ccttaaaaaa aaaaaaaaaa 660 aaaaggccgc ctcggccact ctgcgttgat accactg 697 <210> 13 <211> 653 <212> DNA <213> Oryzias sp. <400> 13 aagcagtggt atcaacgcag agtggccatt acggccgggg tggaggttca gacgccatga 60 agttcttagt gcttgccttg tttgttgctg gtgcctacgg gtgcggcttg cccacctttc 120 cccccatctt gaccagagtg gttggaggac aggacgtcag gcctaacagt tggccatggc 180 agatttccct gcagtataac aacaatggtg agtggagaca cacctgtgga ggtactctga 240 tttctgacca atgggtgctc actgctgctc attgtatcag ttctggaagg gagtacagag 300 ttgccctggg gaagcacaac ctggtggaga cagaggaggg ctctgtcttt aagagcacag 360 ccaacatcat tgtccatgag aactggagcg ccctgttcat tcgaaatgac attgccctga 420 tcaagctgga ttcccctgtt gagttctcag actcaatcat ggctgcttgt ctccccgtgg 480 ctggctttct ccttccccac aatgaatcct gcttcgtcac tgggtggggt cgcatctaca 540 ctggaggtcc cattgcagac atcctgcagc aggctctcct gcctgtggtg gaccacgcga 600 cctgcagcca ggcagactgg tggggcttcc aggtgacaga gagcatggtc tgt 653 <210> 14 <211> 392 <212> DNA <213> Oryzias sp. <400> 14 ttgccaatgg atacgacatt gctcttctga gactttcctc tgacgccgcc ctgaactctt 60 acgtccagct ggcttctctg cctccctctg ggcagattct gcctcacaac aatctctgtt 120 acatcactgg atggggactc acctctactg gtggaagcct gtcctctaag ctgaaggagg 180 cctatcttcc cttggtcgac cacaacacct gctcccgcag tgactggtgg ggcagcactg 240 tgaagaccac catggtgtgt ggaggaggtg gttctgagtc tggatgcaac ggtgactccg 300 gtggccctct gaactgccag gttagcggaa aatactacgt tcatggtatt gctagctttg 360 tgtctggata tggatgcaac tacccccaga ag 392 <210> 15 <211> 495 <212> DNA <213> Oryzias sp. <400> 15 aaatgcacag taccagaaaa catgaggcac cgtttcaggc gacacatgac aaaaagaaca 60 attaaaatca atatcttttt ttaaactttg taaaagataa catttaagaa taattttaaa 120 ggaaatttcc ttaatcttat tagaaacgaa atatttatgg ggcaatgtcc aaactttatc 180 ccactgaatc ttataaccaa atgtattcca ataggagata acgtggggct tagtgataat 240 ctcatttaaa aataatgaac gaatattttt tgttattttt accttagaaa aacagatttt 300 tccacaatat gaatctgtta atgcgggtgg agaacatggt aagttaaaag aggaagtaca 360 gcaaatcaac ttgacaccac taggaataga tctgatgact aatgcaaact cttcagaaga 420 gacagataga ttatatgtgg aaagaaattc gctataagtc attaaatttc catgtggagt 480 tatcagttgc gttac 495 <210> 16 <211> 162 <212> DNA <213> Oryzias sp. <400> 16 catgctgatc aagctgagca cgcccgccag cctgaacagc tatgtgagca ccgtgtctct 60 gccctccagc tgtgcttctg ccggcacctc ctgcctgatc tctggatggg gaaacaccag 120 cggcagtgga agcaactacc ccgaccgcct gatgtgcctg ga 162 <210> 17 <211> 565 <212> DNA <213> Oryzias sp. <400> 17 aagcagtggt atcaacgcag agtgccatta cggccgggga gatgatgcag gcagcgctgc 60 cggcgggact gaaacgatgg tgatccaggt gatcgtgaac acctttgagg gccagaagat 120 aaccgtggac ctgtgtcaag atgagaaaca gctgcagctg atgaccgtgc tgcaactgaa 180 gcagaaaata gcgctaaagc ttcccgggag agcagagcgg atggcggcag acatgcagct 240 gatcttcgcg gataagaggc tggacaagac agcaaactgc tgtgtgaatt cgggatcgtc 300 cacaagtctc tgatccagat ggtgatgtct ctggatggag gaagaggagc atgaccgcag 360 gaaaccagac atccagctgc acattcatca tgcatcaaac agactgttta cttgtttctt 420 ttatgcttta attgccttga tattgttgtc aaattctccc cagttggagt ttgttgcact 480 aataaaagtg gaaaacttag aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 540 aaaaaaaaaa aaaaaaaaaa aaaaa 565 <210> 18 <211> 922 <212> DNA <213> Oryzias sp. <400> 18 aagcagtggt atcaacgcag agtggccatt acggccgggg ggaaaagact ccgaagacaa 60 actgcgcagt tgaatctcca acatgtggac cctcggttct gttctgttct cggttctggt 120 cggttcatct gtttctggat cttcagatct gatcatctct gctgaacctg gacagaacat 180 cactctacca tgtggagatc caaacatcaa cgaggttttt cttttagagt ggatcagaac 240 tgatctgaag aaggatgaat atgtattttt atacagggat aaccatactt atcctgatga 300 tcaacatgaa tctttcaaga atcgcgtgtt tctgaagaac aatcagatga aagatgaaga 360 tctgtctgtg gttctgaaga acctgaagac ggaagattct ggaagatatg aatgtagagt 420 tgttaaagaa gatgatccac agagagaact gaagctgatc tccagcatcc atctgcaggt 480 gtctcctcca gatctgaaca tcactgctaa acctggagag aacgtcactc taccatgtgg 540 agatgcaaag atcaacaagg attcagtttt ggagtggacc agaactgatc tgcagaagga 600 taaagatgtg ttttttagaa tgaacaactc tgcagtttct gaaggtcgac ctgaatcttt 660 caagaatcga gtgattctga agaagaatca ggtgaaggat ggagatctgt ctgtggttct 720 gaagaacctg aagattgaag attctggaac atatgaatgt aaagatatga agagaagaag 780 caggagatca gctgatcaac gtcctccgat cgccatcatc aacctgctgg tgtctcctgg 840 agagaaagaa ggacaacaca aaggaggagg aggagatgga ggcagtcgtg gtcatcttgg 900 tctgattgct gcatcagttt ct 922 <210> 19 <211> 982 <212> DNA <213> Oryzias sp. <400> 19 aagcagtggt atcaacgcag agtggccatt acggccgggg actacagttt gaggacatgg 60 attccctcag gttaagcgtt gccatccttc tggctgtttc cacctgctcc tgggctgcag 120 agaaggaact gtccgtctct gagctcctgt ggaaggccaa caaggatgtc gtgcgttcag 180 gagacgagcc ctttgtggtg gatgacattg cttatgtcag taaatctgac aggaacgctg 240 accagtgcac gtccggcagc tgcttgtggc ccaaatccgc tgatggaagg gtctacatac 300 cctatgtgat cgctaaccat tacacttcta gtgagctcca agtcattgag cgcggcctgc 360 agtcgttctc tgaggtttcc tgcatccggt ttatccctta ttctggccac agagactaca 420 tccacatcca gtcacagaac ggctgttact cctacgtcgg acgcatcggc tacggtcagg 480 cgctgtcgtt gagccgtcag ggctgcatct accacagcac ggttcagcac gaactgctcc 540 acgccctcgg cttccaccac gagcaatgtc gttctgacag ggaccagtac atccgagtcc 600 tgtgggagaa catccagtcc ggtctggagt acgcctttga taaactcaac actgtgaacc 660 tggacacccc ctacgactac aactctgtca tgcagtacca cagatatgcc ttctctgcaa 720 acaacaagcc caccatggag ccctatcctg atcctaacgt tccttttggc acggctaccc 780 agatgagcaa caacgacatc acaagactga acaggctgta caaatgttga gccgtaagaa 840 caagtttgta cagcaagtcc tcaaagtacc gtggaaaggt ttccagatac aaataaatca 900 ataaaggaaa aataagtttg atggttgtag agctcagact ttaaccgcat catgactaaa 960 taaatgtaat acaaagctct ag 982 <210> 20 <211> 334 <212> DNA <213> Oryzias sp. <400> 20 gcagtggtat caacgcagag tagccattac ggccggggat gagtgtgaaa caagaaactt 60 taatcacgac aaagaccgaa atctctgatc tgagacgatc attacaaagc ctggaaatag 120 agctgcagtc tcaactcagt ttgaaatctg caatggaggg cacattgtca gaaacagaag 180 cgcgcttttc cagtcaacca tgagcctcca gcaacaggtg acaagtctag aagaacaatt 240 agtcgtggtg cgcactgaca cggaacgcca acgtcaagaa taccagatgt tgttggacat 300 caggaccagg ctggagatgg agatcgcaga atac 334 <210> 21 <211> 337 <212> DNA <213> Oryzias sp. <400> 21 aagcagtggt atcaacgcag agtgccatta cggccggggc ccccaacgac attgccctca 60 tcaagctgtc cagccccgcc aaactgaacg cccacgtgtc ccccgtctgc cttgctgagt 120 ccgccgattt cttcgcccct ggctcaacct gcgtcacctc tggctggggc ctgacccact 180 acctggctcc cagcactccc aacaagctgc agcaggcctc cctgcccctt ctgtccaacg 240 acgactgcaa gcgctactgg ggcagcaaca tctctgacgt catggtctgt gctggaggag 300 ctggtgccac ttcctgcatg ggagactctg gcggtcc 337 <210> 22 <211> 391 <212> DNA <213> Oryzias sp. <400> 22 atgcaaactc cagccccagt tatgccttca ttgataactt tggatgtatg cttgacggca 60 gaatcacagg ttctgactca aagttcgtgc ctcgaccagc tgagaacaag cttaacttcc 120 agttggaagc tttctggttc cagggtgctg acagcggaat gatctacatc acctgccatg 180 tgaaggcgac atctgctgct tatccccttg acgctgaaca caagcttgct cttacgtcaa 240 tggatggagg gaggtcagtg gtgcagatcc agtttacgcc ccctgtgagt cgagtggatt 300 tacggctcat accaatacag cagtatcacc tggcacctca attattagtg gaggcggtca 360 cggatctgga aaaccttcag actcttcaag g 391 <210> 23 <211> 670 <212> DNA <213> Oryzias sp. <400> 23 aagcagtggt atcaacgcag agtgcgagga gatgctgctc cacgcttttg agggttacaa 60 cgtttgcata tttgcttacg gacaaacggg agcgggcaaa agctacacaa tgatgggccg 120 acaggaacag gaccagcagg ggatcatacc cctgctgtgc gaagaccttt tcacaaagat 180 aaacgatagc aataatgaca acaacatgtc gtactctgtg gaggtgagct acatggagat 240 ctactgtgag cgtgtgcgtg accttctcaa ccccaaaaac aaaggcaacc tgcgagtcag 300 agagcaccct ctgctggggc cgtacgttga ggatctgtcc aaactggccg tcacctccta 360 caatgacatc caggacctga tggactctgg gaacaaggcc aggactgtgg ctgcaacaaa 420 catgaacgag accagcagcc gctctcatgc tgtctttaac atcatcttca cacagaagaa 480 atacgacatg gagacggaca acacgtcaga gaaggtcagt aagattagtc tggtggattt 540 ggctggcagt gagagagcag actcaactgg agccaaagga accagactga aggaaggagc 600 aaatattaat aaatccctga ccacactggg gaaagtcatt tctgctctgg ctgaaattga 660 ctctgctcca 670 <210> 24 <211> 829 <212> DNA <213> Oryzias sp. <400> 24 aagcagtggt atcaacgcag agtgaccatt acggccggga gggatctgtc tgaacagatc 60 ccgagctcat cagcggggac aagtcacact tccacagtat aagctcctcc tgtgttccct gtacgagatt 180 tacaagctc tggaggaaga gctggacagg aactccaacc atgctggtgt tgcacccatt 240 tactttccat ctgaactggc cagactggag tccattgaga aagacctgga gtatttctac 300 agcaccaact ggagggagaa gatcgttgtt cctgctgcta cgaagagata ctgtcacagg 360 atcagacaga ttggcaaaga gaacccagag tttctggtgg cccacgctta cacccgttac 420 ctcggcgatc tgtccggagg ccaggttctt ggtcggatcg cccagaaatc catggggctg 480 aaaaccagcg agggtctgtc tttcttcgcc ttccctggag tctccagccc caacctgttc 540 aagcagctct accgcagccg aatgaacagc gtggagctga cggaggagca gaggaacggc 600 gtgctggagg aggcggtccg gtccttcgag ttcaacatcc aggtctttga tgacatccag 660 aagatgctgg acgtcacaga aaacaagctg cagaacagtt taacagacaa gtcaagagag 720 gctaaaacgc tccacgtccc gctcatcagg atggtgctgg gactttttgt ggctctggct 780 acagtcagca tgggattcta cgctctataa aattagactt ttttttttt 829 <210> 25 <211> 1396 <212> DNA <213> Oryzias sp. <400> 25 aagcagtggt atcaacgcag agtggccatt acggccgggg agaagcttcg ggacgttgga 60 acaggatgaa gtctgtttgg ttgctgttgg tgcttgtagc tgcagtcaaa gcggagagga 120 cttttgttgg tgatcaggtt ttcaggatcc atttgcagtc tcaggaacag ctccgtgttc 180 tccagtctct ggaacatgaa gaggaatggc agctggactt ctggctccac cccgtttcca 240 cagacctgcc agtggatatc cgagttccca gctccagtct gagctccatg aaggagttcc 300 tcctcaccca taacatcccc ttcaccatca tcattgagaa ccttcaggag cttattgata 360 tggagaaaac tgaaatggag gagagccaaa aaatggagaa gagcaccagg agcttcaact 420 ttggagctta tcatactctg gagacaatct acagctggat ggacacactg gtggctcaat 480 acccacactt ggtcaccaaa caagaaatcg gtaaatccta tgagaacagg cccatgtacg 540 tcctgaagtt cagcactggc ggcaccaacc gccccgccat ctggattgac acggggatcc 600 actccagaga atgggtgtct cccgctacag gactgtggac ggctaacaag attgtctctg 660 atttcggcag tgacgcctct ctgacctccc ttttaaacac catggacatt tacatgctga 720 tcctggccaa tcctgatggc tatgtgtaca cccacactaa taaccgaatg tggcgcaaga 780 cccgctccct aaactctggc tcatcgtgtc gtggagtaga tcccaacaga aactgggatg 840 caggcttcgg tggtcctggt gccagtacaa gcccctgctc tgattcctat cacggaccct 900 ttgctcactc tgagattgaa gtgaagaacg tggtggattt gatcaagagc cacggcaatt 960 ttaagtcgtt catctccgtc catgcctact cccagctgct catgtatccc tatggatata 1020 cctgcacaga tgtggcagac aaggctgagc tggattctct tggcagagca gccacacaga 1080 aactcacctc cctctatggc acctattaca aagttggaaa catttgcaac attatctatc 1140 cagcaagcgg aggcagcatt gactggtcct atgatacagg catcaaatac tcgtttgcct 1200 ttgagctgcg ggacaccggc tactatggct tcctcttacc atccaatcag atcatcccca 1260 cggccacaga gacgtggctt gctctaaaac acatcatgga gtatgtgcgt gatcacccgt 1320 actaatggct gcatagtcat ctgtgaattt tagattcagc tttccctctg tgaccaatta 1380 aagtcatcaa aaccct 1396 <210> 26 <211> 576 <212> DNA <213> Oryzias sp. <400> 26 aagcagtggt atcaacgcag agtggccatt acggccgggg acatccgtcc ttctccgtca 60 tacctttacc cccccagagg cgtctcatcc gcagccatga agttctcctg tgttgccgct 120 cttcttgtgg tgctggccgt cactcgtggg tgtgaggccg tgtccctggt gaggcgggac 180 gccccgtccg aggtggacag gctcacccag ctgatggaga acatgtccgc cagcatcgcc 240 gccgccaccc aggaggtggt ggacaagatg aaggcgctcg agatgaccag cgccgcccat 300 gtttccaagc tccagcccct ggtggagcag gtccaggccg aggccgccaa gctgcaggag 360 caggtcaaac cctacgtgtc caacatcgag gagcacatcc gacccctgac cgacaacctc 420 aacagccacg tcaagcctct caccgacatg atggagaagc ttttgcagca ggtggtggac 480 cagtccaaag ccctgctgcc cccccagtag acctgtgtct gttttcttct gtctgtaatt 540 tatctaaccg tgtttgaatt aaaggcttca aaagtt 576 <210> 27 <211> 1486 <212> DNA <213> Oryzias sp. <400> 27 aagcagtggt atcaacgcag agtggccatt acggccgggg actgccaaca tgaaggtcct 60 gctgtttttt ggactggtgg ctcttgccct ggctgacgtc actcgatttg aaggagaaaa 120 agtcctccgt ctgaagcctg tcctcgctga acatgtgacc ctcatcaagg atctggctga 180 gagcatcgaa gtggacttct ggagccctga cagtcctgag ctggtgacta tcgacatcga 240 tgtggacatc cgtgtgcccg ccaagtacct ctccctggtg tacaccaccg tgggtcagag 300 cggcatggag catgagactc tcatcgatga cgttcaggtt gccatcaacc gccaggctga 360 caacgggatc agtcctagaa atctgcacag ctacaccaag tacaacactt gggacactat 420 cctatcctgg atcaacacca tcgcctcctc caaccccagc ctgatcagca agcaggagat 480 cggaaacacg tttgaaggac gccccatgac tctcctcaag attggcaaat cctctggcgc 540 tactaagccg gccatcttta tggactgcgg catccacgcc agagagtgga tctctcctgc 600 tttctgccag tggtttgtga aggaggcttt ggagacctat ggcagtgact ctcagatgac 660 cagcctgctt gaccagatgg acatctacgt cctgcccgta ttcaacatcg acggctacga 720 atactcacac aactctaaca ggatgtggag aaagacccgc tccaagcttt ctggaagcag 780 ctgccttgga gctgatccca acaggaactt tgatgccggc tggtgcaccg tcggagcctc 840 cagtaacccc tgcagcgaca ccttctgtgg ctccaccccc gagtctgaga tcgaggtcaa 900 gaacgtggct aacttcatcc gcaagtacaa gtcctccatc aaagcctacc tcaccgtcca 960 ctcctactcc cagctccttc tcttccccta ctcctactcc tacaacctgg ctgccgacca 1020 cagtgagctg atgagtgttg ctcagggagc ctctgctgct ctgactagtc tgtatggcac 1080 ccgctatacc agtgggcctg gtgcttctac tatctatctt gctgcaggag gttctgatga 1140 ctgggcctat gacctggggg tgaaatattc ctataccttt gagttgcgtg acactggtta 1200 ttatggcttc ctgctgccag agtctcagat caagcccaca tgcgaggaga ccatgctggc 1260 cgtcaagtac attgctgccc atgtgcagaa gaacctctat taaaaagcgt agcagtcagg 1320 acccctgcca acgcaatgcc tctgccccag cccccgagcc cctccaacct ctcagccgtc 1380 tcatgcccgt atctatggat acgggcgcca cccgccatgt tttctcactg tgcttgacag 1440 agtgtcacga tgcaagtgca aacaataaaa tcaatgatcc atcccc 1486 <210> 28 <211> 426 <212> DNA <213> Oryzias sp. <400> 28 taaagtatgc cgtacctgat cctctaaaag aggagaacag ggacacttta gtccccgttg 60 aacaagcaaa cacacatttc ctctttgtag cctcagaaga tgatctcagt ttggacagta 120 gagctttcat gatagagatg atggagagat tgcagcgtct tgggaagaaa aactttgaca 180 gtgtgtgtta tcctaaagct ggacactttc tagagccccc ttatggccca ttctgcccct 240 ccagcttaaa caggtttgta aaaaacatat ctttgtgggg gggagaatca agggcccatg 300 cagcagctga agttgacatg tggaagaaga tccaagagtt cctgaaaagt catgtgagct 360 gtgatcctgt gcactttgat gccaaattat agatttaatg tgagtgttaa taatcataac 420 tatcaa 426 <210> 29 <211> 844 <212> DNA <213> Oryzias sp. <400> 29 aagcagtggt atcaacgcag agtggccatt acggccgggg acatttcaac acgtgctctg 60 ttctgtgttg cacctctatc aaaaaagatc cattatggca actgtggtgg tggtgaatca 120 aggcccggga aactggagca ccgggttgtg cagttgctgc tctgatatgt ctacctgttg 180 ctgctccttg tggtgcttcc cctgcatgca gtgtcagacg gccagccgcc atggctggtg 240 ctgcgccatg ccgctcctgg atgtgttctg cggcgtggtg acctgcgttc tgcagtcctc 300 catcagaaag cgcccaacat ccctggttcg tgctgcgagg actgcctcaa gtgctcgttc 360 tgctacgtgt gcgtctggtg ccagatgaac cgagaggtga agatccgatc caagaccgtc 420 agccccaccg tggtcaccac gcaggtcgcc agagcttagc cggctggagt taaccattcg 480 tttaaggaaa cgccttaaag gggttcaaga aatccataat gtaactgttt ttgttggtat 540 attaaatgtt ttcagtttgt taaagctaca ggaaatgttg taataataat ctttggtttg 600 actccaaatg tttttttctt gcatccagaa aaaaatatga ctttaagttt gtaaacagaa 660 aaaatatctt aaattacata caaattgatt ttgtttatag aaatggtttt atatgtgagt 720 cacggcctgt tttgatggat ccatttaaaa ctgttcgtaa aaggctttcc taacatacgg 780 ttctgtgaaa aaacatttcc cttttcatat ttttatttgt cttaaataaa aagctttgag 840 tcaa 844 <210> 30 <211> 532 <212> DNA <213> Oryzias sp. <400> 30 tgtctccgac gactaagcag tggtatcaac gcagagtgcc attacggccg gggacatctc 60 ctcagatttc ttttctctgc acaccaaaaa ccaagccaaa accatgagtc agctggagac 120 ggccatggcc atgctgatgc agaccttcga cacgtacgcc ggcaaagatg gcaagaagga 180 atcgatgagc aagacagaag tgaagaccat gttggagaag gagctccccg cactcctgaa 240 ggcagcaaag aaccaagctg aggtggacaa gctgctgaag ggcctggact tcaacggaga 300 ctcggaggtg gacttcagcg agttcgtggt gatggtcgcc gctctgacgt gcgctgcgca 360 cggccgctgc tccaagtaga actgagctgc gcccttgggc gggacacgga gacgtcaaca 420 acatgaaatc ctcacggttg cttttgtaac gacagaataa agtgatttac ctacaaaaaa 480 aaaaaaaaaa aaaaaaggcc gcctcggcca ctctgcgttg ataccactgc tt 532 <210> 31 <211> 427 <212> DNA <213> Oryzias sp. <400> 31 aagcagtggt atcaacgcag agtgcgccag tcagccttgt cttaacaacg gcaaatgcat 60 cgacaaaatc aactccttct actgcgagtg ccccaaaggg ttttcaggaa atctgtgtca 120 ggtggatata gatgagtgtg ctagtacgcc ctgcaggaat ggagccaaat gtacagatgg 180 tcccaacaag tataattgtg aatgtgctga aggtatgata tgatggaatt ttgtcaaaag 240 ttgcttgtaa cttttcgttt tcctttgagg gcatgaaaat cttttcatat gtgtgtggag 300 aatttcaaaa ggcatattga catttttgca aaaaatcaaa tttttgtaac acaaatttca 360 tgtttgagct aagagtctca aatgtgtatt aaagggtaac aaataaagat caatttgtga 420 caaaact 427 <210> 32 <211> 701 <212> DNA <213> Oryzias sp. <400> 32 cgttgttggc aaagtctaac ttaacactgg cgtggttgat tcctacaaag acggcgatgc 60 atctcatgac gctggcccac tccctcttga acttgtgggg ctctcccaga tgactgtcga 120 ggcaggggta cagcaggccg ataacagctg ctcctgtccc gcagcaaggt gggatccacc 180 aggcggagga gaacaacgtt gtcatcacct cctcgggaaa cagagtgacg tttctttgta 240 tctgtagtaa attgagcacc agagccagga agaccccgac cgtgaagagg accagacccc 300 ggcggatgag gttggtggtc cggacgtcgt gcagagaccc gtaagcgctc ccgagcaccg 360 tggttatgat ggacatcatc tcctcagcct tggatgcaaa ccagctcgcc ccagatgaat 420 gcttagcgtc agccttggga gaacaagagc agctccagca gtggtcctcc agtctcggca 480 tttggcactt tttcacttga tttctcttca gactgtgacc caaacttccg tcctgctcgt 540 tggcgcctcg gccagtgatc aaactatcgc tctgttttgt tttcccgcac tctacgacaa 600 ggactgcggt cacgttgttt gctcacatca tcgtttcagg aacaaaaaaa aagaaaagaa 660 agagaaaaca cacacacaca cacacagggt tgcttcattc c 701 <210> 33 <211> 440 <212> DNA <213> Oryzias sp. <400> 33 ggatccacca agccagttcc catcaaccct gaccagacgt gtggagatgg atgggtgtgt 60 gagcacagat ggcgtcagat tacgaacatg gtccaattcc gtaatgtggt cagtggacag 120 cctcatgcca actggtggga caacggcaac aatcaggtcg cctttggccg cggaaaccgt 180 ggattcatcg tgttcaacaa tgacgactgg gctctggatg tgacgctgaa cactggcctt 240 cctggcggca cctactgtga cgtcatctct ggcaataaga acggaggcag ctgcagtggg 300 aagcagatca ctgttggggg tgatggccgt gcacacttct acatcaacaa ctcagaagaa 360 gaccctttca ttgctattca tgctgactct aagctgtaaa tgaaaaccgg agacaataaa 420 acctgcaaac taaaaaaaaa 440 <210> 34 <211> 659 <212> DNA <213> Oryzias sp. <400> 34 aagcagtggt atcaacgcag agtgccatta cggccgggga cagaacctga gccagaaccc 60 ggaccggcac aagacgcccg acccagatcc agtatgcagc acgctcgtgg cgccgtcttc 120 gtggttctcg ccgcgctggc tctcagcagc gtgggagtat cctcgcagtc tgacagagac 180 caggaccagt tccagacca ggacctggac ctggagctgc gtcaccaccg gctgctgcag 240 cgagctcgca gcgccgggct gctgtcacag gactggagta aacgggccat cgagaacatg 300 ctggcccgca tgtcggaccc cgaggccgaa cctgagcggg aggccgaggt gatctccatg 360 acctcagggg ggcggggcaa cctggagcgc tcggtggaca ccgtgccccc ccgccaaccg 420 caaagatggc tgcaaaaact tctactggaa aggcctcact tcctgctgat gccccgccca 480 gctgaggctc cgcctccccg acgaccaaca gacccgtcct gatgtcctga cagagaagac 540 caatcagcag atgacctgaa taattaatgt aattatccaa taaagagaga aatacaatac 600 agaaaaaaaa naaaaaaaaa aaaggccgcc tcggccactc tgcgttgata ccactgctt 659 <210> 35 <211> 146 <212> DNA <213> Oryzias sp. <400> 35 caatcagcta ggaactgtgc tgagtcatat ttaggggcgt tctggtacaa aaagtgtcac 60 agcacgaacc ctaacggcgt ttatcgttgg gggagtgatg ctgcctttcg tgggattgga 120 gtggattggt ccacctggaa gggtca 146 <210> 36 <211> 955 <212> DNA <213> Oryzias sp. <400> 36 aagcagtggt atcaacgcag agtggccatt acggccgggg gcagaagcat caatcaacat 60 gatccccatt gtgctggcct cagtcctcat cgctagtgcc cttgggtgcg gcaccccacc 120 cattcaaccc ctcacttccc gcgtggtcaa cggagaggat gccaagcccc acagctggcc 180 ctggcagatc tccctgcagt acgagaggga tggagagtgg aggcacactt gtgggggaac 240 tctgatcgct gccaactggg tgatgactgc tgctcattgc atcaactccg acttctctta 300 cagagtgttt gtaggtaaac acaatctgct ggaggacgaa gtcggttcca aagccatttt 360 cccagagaag attgtcgtcc atgagaactg gaactccttt ttggtggctt taggtaacga 420 cattgccctg attaaactgt ccgagcctgt gactttgagt gatcatgtgc agctggcatg 480 tattccacct gctgagacgg tgctgtccaa cctctacccc tgctacatca ctggatgggg 540 caggctgtac actggaggcc ccattgctga taacttgcag caagctttga tgcccgtggc 600 cgaccacgcc acctgctccc agcccgactg gtggggtttc attgtcagga ccaacatggt 660 gtgcgccggc ggtgatggaa ttgtggctgg atgcaatggt gactcaggcg gcccgctgaa 720 ctgtcaaaat gctcagggtg tctgggaggt gcacggcatt gccagctttg tgtccggtct 780 tggctgcaac tttgagaaga agccaactgt cttcaccaga gtctctgctt tcaatggctg 840 gatcgaccag gttatgataa ataactgaag aaactgaata aatatgccca gaaaaccaca 900 aaaaaaaana aaaaaaaaag gccgcctcgg ccactctgcg ttgataccac tgctt 955 <210> 37 <211> 342 <212> DNA <213> Oryzias sp. <400> 37 gaaggataca aactggaggt gacgggattc actgatggag gagcaggaga ctctctgggt 60 taccacaatg gaaggaagtt ctccaccttt gacaaagacc aggacgtttc atcccagaac 120 tgtgctgagt tatatttagg ggcgttctgg tacaaacagt gtcactacac aaaccctaac 180 ggcgtttatc gctgggggag tgatgctgcc attcgtgcga ttggagtgga gtggtacacc 240 tggaagggat atgactattc tctgaagaga atcagcttca agatccgtcc tgtgaaataa 300 atctgcagga gaaccgttca acaggtccgt ctgtgtggtt cc 342 <210> 38 <211> 146 <212> DNA <213> Oryzias sp. <400> 38 caatcagcta ggaactgtgc tgagtcatat ttaggggcgt tctggtacaa aaagtgtcac 60 agcacgaacc ctaacggcgt ttatcgttgg gggagtgatg ctgcctttcg tgggattgga 120 gtggattggt ccacctggaa gggtca 146 <210> 39 <211> 1215 <212> DNA <213> Oryzias sp. <400> 39 aagcagtggt atcaacgcag agtggccatt acggccgggg acacctccca agtggtacaa 60 cgcacacaca ctcggaggat ttgatgatct gaccttaaga gcagagatat atatcaccag 120 ttagaccatc taatagacct ggatctactg tttcgggaca tcgaggggtc atggatccga 180 tcgtggaagt ggtggctctg attttaggct tcaccgggtg ggtgatggtg ggagtcgccc 240 tccccaaccg ttactggaag gtctcgaccg tggacgggaa tgtcatcacc acctccacca 300 tctacgagaa cctgtggatg tcctgcgcca cggattcgac cggggtgcac aactgcaggg 360 agttcccgtc gctactggct ttaaatggtt acatccaagc ttctcgcgcc ttgatgatcg 420 tgtccatagt gatgggaaca ttcggtctgg tgggggccct cataggaatc cagtgctcca 480 aagcaggggg ggaaaactat cttctcaaag ggaggattgc tggcactgct ggagtcttct 540 ttatacttca aggtttgtgc accatggtgt cggtgtcctg gtatgccttc aacatcacgc 600 aggatttctt tgaccctttc tatccgggga taaggtatga aatcggagaa gggctttaca 660 tcggctggtg ctccggagtg ctcgccatcg ccgggggagt ctgcctcacg tgttcctgca 720 aagtggcgag agaggaaaaa tacaatatcc cgtatcaagc agaggaacgg tttactccgg 780 tgcagcaccg accagaagtg tagtcgccag cacttacgga agaaacgctt acgtctgaag 840 gacacaagag cggggacatt ttcctacgtt tttctttttt tgttgttgtt gctgatgcat 900 gttgtgggtt taaattggaa cttttggtcc atgacacaac tggaaagaac caagcacaac 960 tgcacgtgtt tgacttcaat acaaggacgt ccttttgtaa acattgtaca aagcccacta 1020 cttttcctga atgaatgttt tcgtttttaa ttgatagtgt acgctgaaaa aaaacatata 1080 atcatttgat gatctgagct gaaatgacca gaagtatgaa caaagtcaaa attattcaaa 1140 tgttattttt gtgtgttttt tgtacagttt tgtaaagttc tttaagcaca ataaagggag 1200 ggtttttttg ttgaa 1215 <210> 40 <211> 841 <212> DNA <213> Oryzias sp. <400> 40 gtggctccag cgaggatggc catgccaatg aacagcctgt ctctggtgtc agacttcagt 60 gaagatacta agactccttt gattaaattg caggacacag ctgtggtcag cgtggagaag 120 cagctgatgg atctcatgca ggatgtcagt ctgccaaagt tcaatgagac cttcctggcc 180 aacctgcaga ccctgaggca gcagatcaat gcagcgagtg gcagaccttt gagtcttgga 240 tgcgcaactg gctgatcttc cagatggctc agaagaagcc tcctgctcca actccccagc 300 cagcatccat gatcaagacc aaatgccagc tggaggcagc accggacacc atcgccaaga 360 tcggcacgta caaacctcag tgtgacgagc agggcaaata caagcccatg cagtgctggc 420 acgccaccgg gtactgctgg tgtgtggacg agtctggcaa ccccatcgag gggaccacca 480 tgcgcggtcg ccctgactgc cgtcgaggct ttgcacctta tcgcaagatg gttgcaccca 540 gaatgatgca gaggacttat cttgaggatg atgctctcac aaaagacgag tgaatgaagc 600 cgagccgagc cgaagcgagg agtttcttct cctgatcctg tctttgcaga actgtatatc 660 ctattattgt aaatctgagg cagatctgac aatttgctgt tcaaatcacg ccacctgtcc 720 atgttatcac ttgattttaa tttgctggct gcttcaaaac aatcatgaca aagcttttac 780 gcttctaatg tcttacttct gtcacctttc acagactttt ctgggtgttt ccacttaatt 840 t 841 <210> 41 <211> 1489 <212> DNA <213> Oryzias sp. <400> 41 aagcagtggt atcaacgcag agtggccatt acggccgggg gacaaacaga tagggcggtg 60 aagtggtcga aagcacacca gtggatggtt tgtaacttac acaaagggag gactttactg 120 tgactgtggg aggaagctga ccgcaaaaat gtcaaacaaa atgatcgagc ttgaggagaa 180 tgcgacccac acaggtccaa aaggagtcat caatgactgg aggaggttta aactggaaag 240 tatggaccag gaaaacttgc cttctgcaaa aaaagaactg ctgagacaaa tgtcttcccc 300 caataaatca aaagatgact ccagagcaaa cctaaaccgc aagatgagtg tccaagagta 360 tgagctgttg aaggaagagg atgaagggtg tctgaagaag tacagaaggc aatgcatgga 420 agaaatgcac aacaagctca gcttcgggcc caagtttgaa ggcgtacacg atctggacag 480 tggagaggcc ttcctagaag tcatagagaa ggagcatcac agcacggtgg tggttgtcca 540 catctataag attggtatca aaggttgtga gcagctcaac agctgccttg actgcctggc 600 aactgagtac cccaccgtaa agttctgcag gatcgacgct gttgcgtctg gagccgctga 660 gcgcttctcg gatgaatatc tacctactct gctggtgtac aaagcaggag agctactagg 720 gaatttcctc tcctgcacac agcacctaag tgaagaattc tttgccacag acgtggaagc 780 ctttctcaat agctatggtc tgctgccaga aaaagagctg cccaagctgg aggatgaaga 840 agaaaatgat gtagagtaaa tcaagtgtgg ccactgtcag tgaaaagaag cagtgtcaaa 900 gatccaagac tgggaagatt catacataaa atatgatgag tattctttat tgccttagca 960 aataggaaat ccatatcatc ttctctagaa ctctagttct ggattattct ctaaaggaga 1020 cacaacaatg gcagccttaa aacaccaaat atgggagctg ctaattgact gaaggataac 1080 aaaacaacta aaacagtttt attttttaat tttgtaattt tagataatgt tacatactgt 1140 gtctttgtaa aaaggattca tacttatctt tttttagtct tagtttatag atatttaata 1200 ttgaaaatga ttttaaaata gtttgatata ttgttcctaa attatcttga acgtatagta 1260 caattcccat gtttctatgg catcactgta atgttctgct agaacttaat acatgaagga 1320 ctgaaatgaa ccataaaaac gttgtttgat tgtatatgtt aaagtagtga agttgactgt 1380 tgtctctgga gaacatttat gagaaacaaa ttaatcaata aaacatgaca actgaggaac 1440 aaaaaaaaaa aaaggccgcc tcggccactc tgcgttgata ccactgctt 1489 <210> 42 <211> 848 <212> DNA <213> Oryzias sp. <400> 42 aagcagtggt atcaacgcag agtggccatt acggccgggg ggacagtcca ggatccgacc 60 tcaacaatgt tcgctccgct tttcgtgtgt gccctgggcc tgattgcggc ccctcaggtg 120 tttggggtct tggaagagtt tcaactcaaa gatgaggctg ttactcctta ctctccgagt 180 gagattgtga acaagcacaa tgacctgagg aggagcgttc agcccacagc cagcaacatg 240 atgaaaatga aatggaacac tgaagctgaa gccaatgcta agaagtgggc caatacctgc 300 tccatgaccc acagccccgc cagctccaga gaaatcagca ccagtggttg tggagagaac 360 ctttacatgt ccagtttcca gaactcgtgg agcaacgcga tccaggcctg gtacaacgag 420 gtgaaggatt tccagtatgg agtgggatcc atcaacggag gcgtggtcgg acattacacc 480 caggtcgttt ggcacaactc taaccagatc ggctgtgcca tggcttactg tcccaactcc 540 acctacaaat acttctatgt ctgccactac tgcccaccag gaaactacca gctgtctcgg 600 ccttacaagt cgggaccctc ctgcgccgac tgcccctccg cctgcgagaa caaattgtgc 660 ttgaggtagc tccgagggag cggggggacc gcacagtcac caaccgcaca gtcaccagga 720 tgggggcgtt tttgcgtcca atcagatcct ctgactccga ttcaataaag acatcaatcc 780 ttagaaaaaa aaaaaaaaaa aaaaaaaaaa aaggccgcct cggccactct gcgttgatac 840 cactgctt 848 <210> 43 <211> 473 <212> DNA <213> Oryzias sp. <400> 43 catggaagac tttgaaggaa acaaggcgta cgctcgctac tcctccttct ctgttggtcc 60 agaggttgaa ggatacaaac tggaggtgac gggattcact gatggaggag caggagactc 120 tctgggttac cacaatggaa tgaagttctc cacctttgac aaagaccagg acgttttatc 180 tagcaactgt gctgaattat atttaggggc gttctggtat agagattgtc accacacaaa 240 ccctaacggc gtttatcgtt gggggagtga tgctgccatt cgtgcgattg gagtggagtg 300 gtacacctgg aagggatatg actattccct gaagagaatc agcttcaaga tccgtcctgt 360 gaaataaatc tgcaggagaa acgactaaca ggtccgtctg tgtggaacat cgaaagaatc 420 attcagaaat ggaagagaga tcagatgatg atagagagga gaagcaaagg aat 473 <210> 44 <211> 146 <212> DNA <213> Oryzias sp. <400> 44 attgtatcta tcaactgtgc taagtcatat ttaggggcgt tctggtacaa aaagtgtcac 60 cgcacgaacc ctaacggcgt ttatcgttgg gggagtgatg ctgcctttcg tgggattgga 120 gtggagtggt acgcctggaa gggtaa 146 <210> 45 <211> 693 <212> DNA <213> Oryzias sp. <400> 45 tccagcagga tgaccacaaa ccaaagtcat tgagcaacta ttaaataagt tcaagatcga 60 gaatgatcca gaggagtttg ctctatattg cattcatcag agtggngaaa agaggaagct 120 gagcaacaaa gatctgccgc tgtgggagcg cgttctgtac gggccctctg atgacatcat 180 gaagatcttt ctgatggaca tgcatgaaga ggaagtcagc gatgatgttg ctcagtatct 240 gaacttggag cgttcaatac tggagcaggt tcttctgcag ctcagagagg aagaaaacag 300 agaaatagag agtgtcatca tcaagtacca gcaccagcac agacttctgt ctcaggtgct 360 gaactccaaa atgtcccctc acatcgagac cactgtgtga cagcaccatc catccatcca 420 tattgaaacc tgctcattca cggtcacagg gttgctggag cctatccagc tatggttggt 480 atcaacgcag agtatacccc gggctgtttg ctaatccatt gtagatggat taattactac 540 accacccttc aactcaagga acaatttgct tttttttttt tttcataaaa tgttaatttt 600 aaatttagat agttaaaaat aaagtttggt gttgttctac ttttgttaca tgctctttta 660 aacacatgtt gcttctgtat taagttttac att 693 <210> 46 <211> 1009 <212> DNA <213> Oryzias sp. <400> 46 aagcagtggt atcaacgcag agtggccatt acggccgggg acgtcagatt acaccttaca 60 ggtgctcgtg ctcatcatga gggttcttgc cgcgatcctc gctctggctg tcctctcagg 120 gtcccacgcc cgcagcgtgc ccctggagga cctgcagaac acgtgggagg tgaccgtgga 180 gaagttccag aattacatca cagacctgaa cacaaaggct gacggcgtgc tggagaacat 240 caggagctct cagatcagca gagagttgga aactctgatc caggacagca tgtcagagct 300 ggccatgtac agagacgacc tgcagaacaa gctgggcccc tacacccagc agagcgcgga 360 gcgcctgagc caggacctgc agctgatggc ggacaaactg cgcgagaaca tgaatgaggc 420 gcgcgagcag gtggaccgct acgtccagga gctgcagacc atgatggagc agaacgccga 480 cgacgtcaag ctcagggtgt ccacctacag ccgcaagctg aagaagcgcc tcaacaagga 540 cacgcaggag atcaagaggt acgtcagcga gtacgtcgag gagctccact ctcgcacctc 600 cgagaatgtg ggagagctga aagcccgcct ggatccgtac ttctctcagg tccgggacaa 660 cgcccaggcc aagatctcca ccctgaacga gctgctccag tcccagatcg agaacgtgaa 720 gaccagggtg cagaccaccc acgaggacat caaggagcgc gttaaggtcg ccaccgagga 780 cctgcgctct tctgtggagg gaaggattca ggaggtgcgc aactggttcc agccctacgt 840 gtccatggtc acggagagcc tgtagataag actggtgacg acccctcatg gaaacatgtt 900 cagactgaaa ccaacaagtc tgtggggaag catgaaatat taaaagatac taaaaacaaa 960 aaaaaaaaaa aaggccgacc tcggccactc tgcgttgata ccactgctt 1009 <210> 47 <211> 394 <212> DNA <213> Oryzias sp. <400> 47 tgctgtggat caacagtact gggagagtca aactcaagcc tctctgggtg cacagcagac 60 ctacaaagcc aacattgaaa tagcaaagca gcgcttcaac caaactggag gtgttcacgt 120 gttccagaac atgtacggat gtgagtggga tgatgagact ggagaggtca aaggctatga 180 tcagtatggt tatgatggag aagacttcat taatcttgac caaaaggaag aatcatgggt 240 tgctcctaaa caacaggctt tgattaccaa acagaagtgg gaccattcga gagcttttac 300 agcatctgaa aagcaatact taacacagga gtgtcctgag tggctgaaga aatatgtgga 360 ctctggaagg agttctctga tgaagaaagt gttt 394 <210> 48 <211> 828 <212> DNA <213> Oryzias sp. <400> 48 gaaggacatg tgggtggagc cggagttgct ggaggccctg aacgaagagc agaagaagat 60 cctcttcctg aagatgagag aggagcaggt gcgccgctgg accgagaggg aacagaggga 120 agagaaagaa ggacggagca acgcagagac caagaaagct acaagtaaac gcgtgtcgtg 180 gcttctcggc cgtgatgggg acgtgagcgt cagcgtgata ggagaggtgg acgaattcag 240 gtcctccaaa atcctgcaga acctccagac cagactccac actgataata tgaatggcat 300 ccagacggcg gacttgctgc caggaagaga ggcccagcag ctcagcttca aagacaacgt 360 caagctgccc ctcgcagatg tgaacgaaga accggtttcg tcgaacggcc aatcgtctga 420 ggagggtttt agtgccgacg gcgacctgaa agaccccgca aaggacggtc gcgtaacaga 480 gctaaagaag acgttcgcag accatcccca caacacatca cctgcccgcc ccagaccgct 540 catacctgac aaaccggctc acctacagag gacggcgcgc agttgacggc tgtaggactg 600 catccaccct gctgcaagac gacactcgac cctcacagag ccaaaggtta cattactgag 660 cgtgctgagc tccacgcaag cgtcacagct cgcttaggaa aacagccaaa gcatcgatga 720 actgaacatc actgcaagat ttgatgtcta gatctgacct gaatgggaag attccagatt 780 aaatttgagc tcaaacactt gcagagacac aaaagctttg gcttaaat 828 <210> 49 <211> 1735 <212> DNA <213> Oryzias sp. <400> 49 aagcagtggt atcaacgcag agtggccatt acggccgggg agagcaactt cgagtagcaa 60 agtagcaact tcaactccct ctcaaggaac tttctcaaag gcagaaaaaa ggaattttac 120 ccacttccga gtggagtttt actggaatct ttcttcttgt tttttcgggg aaatatgatg 180 ttcaccgcct tcaacacaga gtgcgactct tcctcccgct gcagcaccgc ttctccgtcc 240 ggagacaacc tgggatacta cccgtcacct gcgggatctt actccagcat gggatctcct 300 cagtctcagg atttcactga cctgacagca tcaagtgcct ccttcatccc caccgtcaca 360 gccatttcaa caagtccaga cctgcagtgg atggtccagc ctgtggtctc ctcagtggct 420 ccctcccaca gagtccaccc ctacagctcc agccccgctt accccagatc atccatgagc 480 tctccgtcca agatttgctc ttccagacgt ggcaaagtag aaaagataag ccctgaggag 540 gaggagaaga gaagaatccg cagagagaga aataagcagg cagcagctaa atgccgcaac 600 aggaggcgag agctcacaga cacccttcaa gctgaaactg accagcttga agatgagaag 660 tccagcctgc agaacgatat tgctaatctg ctgaaggaga aagaacggct ggagttcatc 720 ctggctgccc accagcccat ctgcaaaatc ccatcagagc tggagagcga gttccccctg 780 acctccatct cctccaccct tccatgcctc tccaccacag tgtcttccac gaccaccttc 840 acctccagcc agcaaaccat acctccaact tccaactcta tcttctccag ctccatcctc 900 tccaccacca cctcaacaac caccaccact gcctctgaca gcacagtcaa gatgagcgac 960 ctcgaggctt ccattctgga ggagtctttg gacctgctgg aaaaaacgga catgaagaca 1020 gcgcagtctg tgccagaggt tgacctttcc agctctcttt acagtaatca agaatgggag 1080 cctcttcatg cctcttcagc caacgtcgac tttgagcccc tgtgcacacc agtggtgact 1140 tgcactccgg cctgcacaac caacacgttc tctctggtgt tcaacttccc agaagctgag 1200 accttcccca cctgtggcgt tgcacacagg agagggagca acagcaatga ccattcctca 1260 gactcgctaa actcacccac cctgctcgct ctttaaggac attttctatt aaaaaaaaaa 1320 tccttcctat caagtcttga aaaattagca gaggcatgag ctttggaaca gatggcaaat 1380 cctatgttac ttgcctttat ctctacttgc ctattaacac caatgtagca agttattgag 1440 catgatttaa acaaaaaaaa cacattgttg ttactattat atttatggtg ctatagatga 1500 caatacttgt tgtgttttca gagcaatagt tatatttttg gtttgttttc tggttatttg 1560 aatgtcaaag taaatttttt tctcccatta aactgctgtg gtgccatggt ctgcaagtcc 1620 atcagactgt gaagttttct gtgaaaacat tagtgcttgt aatttattaa atttctattt 1680 atttattttt tacagacgaa tgttgtgtaa ataaaacata tatatgaaat gaacg 1735 <210> 50 <211> 1210 <212> DNA <213> Oryzias sp. <400> 50 tcaacgcaga gtggcattac ggccggggct ttccaaatgt tttgttccaa agttcactga 60 acctgtaaca tttaaaagac actgttcatg gggacatgaa acattacacc caaaggttat 120 cacaccttct cctcaccatg aaaaccttga ctctggcttt ggtgttttgt gcagtgttat 180 cactggctaa tgctcactgc ttctttatgc gtgtcgatcc tgacgcaact cattgtcagg 240 atgagtcgga tgggagctgg catgcagtgg ggtcctcatg gataagtaac tgcatgaact 300 gcacctgctt ctcatgttgc tctacgtacg ccattccatg gattttcccc gaagaggact 360 gtgaatctgt ctttgacaat tcaacgtgtg aatacattgt gcgaagaaaa gatgatccat 420 ctgtgctgtg tccagtcacg ggtgcagtag ggaaatgaag ccattgttga catgttttac 480 cacaatgtca gatacatttt tataaattgt gctctaagca agatataatt acccaaatta 540 agttacattt attaatttgt atagtttaaa tgacatattc acatgcctac tgcaatatga 600 ctattataaa tacaaataaa atctaatgtt tgttgtatct acttcaactg tttagaagtg 660 tgaagtacca attacattta taaccagaga aaagtaagat cctgcatgtg aagatttttg 720 aaaaacaaag taaaaaaaaa tagtttttga ttaaataaag acttgatgcc cacgaatata 780 atagtcgttc cacagaaata ctttggaaac acttgtatac acaacttttt gtataatttt 840 atgttttaga acagaaaagt attttttgga ttgatacatt taaaaaagaa agttaaaagt 900 aaagtgaaga aatatgttaa catctgtgta gtttgctctt ctcaaaatta actgttttat 960 aattgacaca cgacaaaatc attatgtcaa attgaatgat ttgcaagtca aatactgagc 1020 aaatattaaa caaaaattgt cacttcatga catttcagaa agaaattata ttagtgatgg 1080 atgagatcta aactacaaca atatcactga caaagtattt ttctaagaat ttcagaccat 1140 ggctgctgtg aaaatacttt ccaacaagat tattttccag gttttcaaaa aaaatgtcat 1200 tctgaatctc 1210 <210> 51 <211> 692 <212> DNA <213> Oryzias sp. <400> 51 gcagtggtat caacgcagag tggccattac ggccgggggc agtctctgcc tgttcaacaa 60 ggaacagtca accagaagac tgtgttccag aaactgccga agattgttac aaacctgcgg 120 acatcatgcc cagaaacctg ctgatgaacc cttgtggtga agatgaccta aaattctggg 180 agctggtgga caatgaaggg gacggatgga aagtggagga catgcagggg gacgacggtc 240 aagacatccg taatggggaa gtgaaaaagt attttgcgac ttcttttgat ttgtgttcaa 300 agatgcaagt gatcgatctg ttgactgaag attacacctc agagcaactg gacgctcagc 360 cagaggtcac ggtggaggac tggttctgca gtcgaagaga ctgcggctgc gagtaccagt 420 tggccgtgag cttgctagat aaaacaacgt gatccttcag gagttcctac cagagcgggt 480 ggtcatagac cctgagaagg acgacggctc ctggaagcag accaaacata cattttctgg 540 atacggccct ggcttgcgct tcatctcctt tgaacatgga gggaaagaca ccaagtactg 600 gaagggttgg tatggagttc gggtgactgg gagctccgtt accatcaacc tgtgaactgg 660 tttaatccgg aaatagagaa aagaaaaaca gt 692 <210> 52 <211> 692 <212> DNA <213> Oryzias sp. <400> 52 gcagtggtat caacgcagag tggccattac ggccgggggc agtctctgcc tgttcaacaa 60 ggaacagtca accagaagac tgtgttccag aaactgccga agattgttac aaacctgcgg 120 acatcatgcc cagaaacctg ctgatgaacc cttgtggtga agatgaccta aaattctggg 180 agctggtgga caatgaaggg gacggatgga aagtggagga catgcagggg gacgacggtc 240 aagacatccg taatggggaa gtgaaaaagt attttgcgac ttcttttgat ttgtgttcaa 300 agatgcaagt gatcgatctg ttgactgaag attacacctc agagcaactg gacgctcagc 360 cagaggtcac ggtggaggac tggttctgca gtcgaagaga ctgcggctgc gagtaccagt 420 tggccgtgag cttgctagat aaaacaacgt gatccttcag gagttcctac cagagcgggt 480 ggtcatagac cctgagaagg acgacggctc ctggaagcag accaaacata cattttctgg 540 atacggccct ggcttgcgct tcatctcctt tgaacatgga gggaaagaca ccaagtactg 600 gaagggttgg tatggagttc gggtgactgg gagctccgtt accatcaacc tgtgaactgg 660 tttaatccgg aaatagagaa aagaaaaaca gt 692 <210> 53 <211> 415 <212> DNA <213> Oryzias sp. <400> 53 aagcagtggt atcaacgcag agtggccatt acggccgggg agtctgttct gaatatcaca 60 ggattacaaa atgagagctg cagtgacctt cattctcctc ctgatagctt tacatcaagg 120 tgaggctctg gaatgtaact tctgcttctc cacgggaggg gaactgtgca ctcccaccag 180 cactcagatc tgctccagaa cccagaatgc ctgtggtgcc gtcattttcc aaggagctct 240 caaccgttcg tttcgtcagt gcatgaacat ggcggtgtgt cagggctaca tctccactcc 300 tggaattctg gccacatgct gcagcactga cctctgtaac tgagactgtc gtcagagcct 360 gaagacactt ctgaccttcc agcttcacgc tagtttgatt cctctgaaga aattc 415 <210> 54 <211> 1585 <212> DNA <213> Oryzias sp. <400> 54 aagcagtggt atcaacgcag agtggccatt acggccgggg ctactcggac tgaagaacta 60 tcatccgtaa gagacctcca ctccacctaa gcagcagcag tcatggcaga cctccaagtt 120 ggaatcaatg gctttggccg cattggccgc ctggtgctga gggcctgcct ccagaaaggc 180 atcaaagttg tggccatcaa cgatcccttc attgacctgc agtacatggt ctatatgttc 240 aaatatgatt cactcatgga cgttaccacg gagatgtctc ccacgaggat ggcaagctga 300 tcgtcgatgg aaacgccatc tctgtcttcc agtgcatgaa ccagctgaga tcccctgggg 360 tagcgctgga gccaaatacg tagtcgagtc cactggagtg tttctcagtg tggaaaaggc 420 ctcacttcac atccagggtg gggcacagcg cgtggtcgtg tctgccccct cacccgacgc 480 tccaatgttt gtcatgggag tcaacgaaga caaatacgac ccctccagca tgaccatcgt 540 cagcaatgcc tcctgtacca ctaactgcct ggcccccctg gctaaagtca ttcatgataa 600 ctttggcatt gaggaggccc tcatgactac agtccatgcg tacacagcca ctcagaagac 660 agtggacggt cccagcgcca aggcctggcg tgatggacgt ggtgcccacg ctaacataat 720 tccagcttcc actggtgctg ccaaagcagt gggcaaagtc atccctgctc tcaacggaaa 780 gctgacaggt atggcgttca gggtgccggt ggctgatgtg tctgtggtag atctaacatg 840 ccgcctgtcc aagcccgcat cttatggtga gatcaaggag gccgttaaga aggctgcaca 900 tggacccatg aagggtgtgc tgggatacac tgaagaccag gtggtgtcct ctgatttcat 960 tggtgacact cactcttcca tctttgatgc tggtgcaggc atctctctca atgacaactt 1020 tgtcaagctc atttcctggt atgacaatga gtatggctac agcaaccgcg tggctgacct 1080 gctgctgtac atgcactcca aggagtagac acttcctgcc cagagaggaa gttaggaaag 1140 ggaccacccc aaccctcctc attaccgccc tgctctttta cacaccagcc cacccatagc 1200 caagtcatag cgtccccgcc cttagctcta ctacagtata tgtaggcggc agcaacagtg 1260 tttgaatgtt ttgagttcat ctgtctcttc ctttttcatt acgaggcgac cggtcggtaa 1320 cagtgttttg tgtccgtgtg cccctttaaa gctccttctg cccttctaga tgttgttatg 1380 actgagttct gtggtgacta ctgtatccat ctcactgtgg ttgtgtttta aaagcttatt 1440 caccagtgga aggaggaagt tgaaaggcag gccttgtgtt atcctgcttt aaagtttaac 1500 gagaccaaga aataaagttt gaaaaaacct caaaaaaaaa aaaaaaaaaa ggccgcctcg 1560 gccactctgc gttgatacca ctgct 1585 <210> 55 <211> 466 <212> DNA <213> Oryzias sp. <400> 55 aagcagtggt atcaacgcag agtcagccga ggtcaacaga ctgacaaggt tggcgatcaa 60 tcggccttct cggtcatcag gaacatcacc acctatcctc ccttcgctca ggttgttaaa 120 gactggatcg atgtggtcaa catcccaggt cattttaaaa ccgacacagg cgagttcatc 180 tgtgacatat ctggagttta ttacttcaac tttcactccg ttgccaaggt cagcatgtgc 240 ctggccatcg tcagcaccgt tctggacaac aagctggggt tctgtgatta cagcaaaggt 300 ggtgaacagg tgctctctgg gggcgtggtc ttacagctga cagcaggtga caaggtgtgg 360 ctcgagtcct ttagagacca gcaagaggcc aaagtcatgg ataatagtga gagcaaacag 420 atcatcttca acggcttcct gatcttcaca gcccaatagc tcagat 466 <210> 56 <211> 1563 <212> DNA <213> Oryzias sp. <400> 56 ccttcagagg ggagctgaga gaaaaggctc tggagatggg agccaagtac cgacccgact 60 ggacgcctga ctcaacacat ctcatctgcg ccttcgccaa caccccaaag tacagccagg 120 tgaagtcggc gggggggctc atcgtacgga aggagtgggt gttggattgc cacaagagga 180 agcagaagat ctcctataaa cgttatttga tggatggaga cgagtcgagc tccgaaagcg 240 aaatggaggt tgacgaagat agcgaggaag aagtcaagac aaagactccg cagaagcagg 300 aaagacaagt cactccgaag aaaactccag caaagaggga tgaagaggag gagtacgctg 360 gatccactga tgtggaggaa ccaggagctg aggacgatga atcaggtgtg gacacagagg 420 atgagctgca gagggtggag aacgagagca ggaggaagaa agcagcggca gaaacgacag 480 tgaacgaaga ggatccttac ggggggtcga cagacgaaaa caccgacatt gaagctgagg 540 aggatcatcc catccccgag ctaccagatt tcctgagtgg aaagcgattt ttcctgtacg 600 gaaaattccc caacgatgag cggcgactgc tgttgcggta catcgttgcc tttaatgggg 660 tgattgagga ctacatgaca gagaaagtgc agtttgtggt gaccagtgaa gggtggcacg 720 attcctttga agatgccctg atggaaaacg gcaatctgaa cttcgtgaag cctgcttgga 780 tttatgccat caacgagcga cagaaactgc tgccctacca gccttacacc gtcgtcccct 840 gagctgcacc cctccctgaa catcctcaca aactgaggtt tgcaaagcca cgtttcacct 900 caaaagggca ataaatcagc gtctcttctt tatgtctcta cactgacttc acacctttag 960 gttaatctct tctacagaaa aatgtgtttg tgtcccttaa ggattcatta aaaatagatt 1020 tatttttagc tcccccatta aaggctggtc agccgaagcc tgtccgagca ctagaataac 1080 atttttcttg cgccacgttt cactgggaca ccatggaaaa agtcaaatct ctgttttttg 1140 tgtgtgtgta cacgtctctg ctcttcctcg gctacctcag gctacacggg ggaatgagta 1200 atcggcctgt cctgactgcc actcgcggcg ataagactca gaatacagtt gaattaatga 1260 gactaaacgc agaagccaca ggaaaactgc tgctagtttt tctacacaac aataaaaaaa 1320 tagtttaaat gaagacaaaa gcaattgaat ttaagtagta gaggcttcat ttttttttct 1380 ttgtgtgtta gagtttgatg ggtttgtttt attaatattt gtgttataaa tctaccagag 1440 acagcaataa gaacatacat tgtctaattc atgtggaaac atttgaaact ttgcaaagca 1500 gtttttaact ctcaaacgtt tttttattat tattattatt attatattgt tgtacctaat 1560 taa 1563 <210> 57 <211> 478 <212> DNA <213> Oryzias sp. <400> 57 aagcagtggt atcaacgcag agtgctgtgt cctgctaccg aattgagccg gggacaatag 60 ctgggattgt ctgcgtggat gtgctgctga ccctcatcat cgtagctgtc acctaccgct 120 gtgccagtta tagacataag aaagtagaaa atgctaacaa agtgtatatg aacgtccggg 180 ccaactgcaa gaaaacataa aaatggactc cacagttttt ggctttttaa cttgtttttt 240 attttctttc aatagatata acaaacttgt ataccaggtt acttaaaaaa acttgctcat 300 aagacatttt taatatatgt ttgctcaaat taaccatatc tcttgaataa agaattttga 360 aaattaaaga agcccattat ataaactatt attactttca ttcaaaatgt ctttgtcttt 420 tgtctgaaat gtttgctttt ttgaggtcct tgcataaata aatattccat atctacct 478 <210> 58 <211> 1055 <212> DNA <213> Oryzias sp. <400> 58 aagcagtggt atcaacgcag aggccgagtg ggcggttcaa atcctgaaga ccagcgggaa 60 gcccgtggcc gcgactctgt gcatcggacc ggagggggac ctgaacggag tcagccccgg 120 agactgcgct gtcaagctgg tgaaggctgg tgcagatatc gtgggggtaa actgccaatt 180 tgacccggag agctgcgtga agaccgtgaa gatgatgaag gagggcgtgg agaaggcggg 240 gcttaaagcc cactacatga gccagccgct ggccatgcac acccccgact gcaatcgcca 300 gggcttcatc gacctgccgg agtttccgtt cgctttggag cccagagcgt tgaccagatg 360 ggacgtgcac aggtacgcgc gggaagccta caacgccggc atccgttaca tcggcggctg 420 ctgcgggttc gagccgtacc acatccgagc cgtggccgag gagctggcgc ccgagcgcgg 480 tttcctgccg cccgcttccg acaagcacgg cgtgtggggc gccggcctgg agatgcacac 540 caagccttgg gtcagggcca gggctcgccg tgaatactgg gagaatctga agcctgcatc 600 tggacgcccg ttctgccccg ccatgtccaa accggactgc tggggctact ccaaaggcca 660 ccccgacctg atgcagcaga aggaggccac cagcgaggaa cagctcaagg ccgtctttga 720 aaaagctgcc gaaaacaaat gagagccccc cacccccccg agggtcacaa tgaattcaga 780 tcaattcaga agttgacccc cccacctaag ccgaagagat atgaaacttc cctgagcctg 840 acccgcagga acaactagtt tttaaagctt tgtttaggaa catttctgtc tttttgtagt 900 gtttttagcc gatttgtact ttaaaagctg cttagattaa ctgctgaagt aaagtcctaa 960 ccaccgagtg atgttatgat gcaaaaataa agctctgcta agaaaaaaaaaaaaaaaaa 1020 aaaggccgcc tcggccactc tgcgttgata ccact 1055 <210> 59 <211> 1196 <212> DNA <213> Oryzias sp. <400> 59 gagtcggcat cctgagctgg caccccacgg ctcacaacgt tctgatgagt gcaggctgtg 60 ataacgtggt catcctgtgg aacgtggccc gcggggaggc ggccgtgagg atcgacaccg 120 ttcacactga cctcatctac tgcgcctgct ggaaccggga cgggtcccag atcctcacct 180 cctgcaaaga caaggcgctg cgagtgctgg acccccgcaa gggcaccgtc ctctatgagc 240 gggaaaagcc tcacgagggt tccaggccca ttcgagccat tttcacgtct gacgggaaga 300 tcctgagcac cggtttcagc cggatgagcg agaggcaggt ggcgctgtgg gatccgaaaa 360 acttctcgga ggcgctgact ctgcaggagc tggacaccag cagcggggtt ctgctgccct 420 tctatgaccc agacaccaac gtggtctacc tgtgtggcaa gggagacagc agcatccgct 480 actttgaagt gacggacgag gctccttaca tccactacct gtccatttac agcagcaagg 540 agagtcagaa ggggatgggc tacatgccca agagggggct ggagatcaac aaatgtgaaa 600 tcgccaggtt ttacaaactg cacgagagga agtgtgagcc cattgtgatg accgtacctc 660 gcaagtcgga tctgttccag gaggatctgt accccgacac cctcgggccc gagccctcgg 720 ccgaggccga cgagtggttt gcaggaaaag acgctcctcc ggttttgatt tctctgaagg 780 acggctttgt ggcgacgacc aaagccaagg agttcaaagt tcacaagagt cttctgaaga 840 ccacgagcgc ttcagcggcg agtcagccgg acgccagcgg ggaggtgcag tccttgaaga 900 aggaggtgaa ggacctgaaa gctgcgatgg aggagctgac caagcgcgtg atggagctgg 960 agagcaaaga ttaaagagga aagacgagaa aacgaagcca aaaatctgga tgaaagagca 1020 ggagcacgac ctgaaactgt tgttcgcttt gggtggaagt gagacaaagg gatagattag 1080 gaaacatttc cagtttgaca attcatttgt ttgattctag tgaccaattt ttacaaaaaa 1140 aagaaaaatc ctttttttaa tatatgattt tatttgaaat aaagtctttc gtgttt 1196 <210> 60 <211> 1489 <212> DNA <213> Oryzias sp. <400> 60 aagcagtggt atcaacgcag agtggccatt acggccgggg gacaaacaga tagggcggtg 60 aagtggtcga aagcacacca gtggatggtt tgtaacttac acaaagggag gactttactg 120 tgactgtggg aggaagctga ccgcaaaaat gtcaaacaaa atgatcgagc ttgaggagaa 180 tgcgacccac acaggtccaa aaggagtcat caatgactgg aggaggttta aactggaaag 240 tatggaccag gaaaacttgc cttctgcaaa aaaagaactg ctgagacaaa tgtcttcccc 300 caataaatca aaagatgact ccagagcaaa cctaaaccgc aagatgagtg tccaagagta 360 tgagctgttg aaggaagagg atgaagggtg tctgaagaag tacagaaggc aatgcatgga 420 agaaatgcac aacaagctca gcttcgggcc caagtttgaa ggcgtacacg atctggacag 480 tggagaggcc ttcctagaag tcatagagaa ggagcatcac agcacggtgg tggttgtcca 540 catctataag attggtatca aaggttgtga gcagctcaac agctgccttg actgcctggc 600 aactgagtac cccaccgtaa agttctgcag gatcgacgct gttgcgtctg gagccgctga 660 gcgcttctcg gatgaatatc tacctactct gctggtgtac aaagcaggag agctactagg 720 gaatttcctc tcctgcacac agcacctaag tgaagaattc tttgccacag acgtggaagc 780 ctttctcaat agctatggtc tgctgccaga aaaagagctg cccaagctgg aggatgaaga 840 agaaaatgat gtagagtaaa tcaagtgtgg ccactgtcag tgaaaagaag cagtgtcaaa 900 gatccaagac tgggaagatt catacataaa atatgatgag tattctttat tgccttagca 960 aataggaaat ccatatcatc ttctctagaa ctctagttct ggattattct ctaaaggaga 1020 cacaacaatg gcagccttaa aacaccaaat atgggagctg ctaattgact gaaggataac 1080 aaaacaacta aaacagtttt attttttaat tttgtaattt tagataatgt tacatactgt 1140 gtctttgtaa aaaggattca tacttatctt tttttagtct tagtttatag atatttaata 1200 ttgaaaatga ttttaaaata gtttgatata ttgttcctaa attatcttga acgtatagta 1260 caattcccat gtttctatgg catcactgta atgttctgct agaacttaat acatgaagga 1320 ctgaaatgaa ccataaaaac gttgtttgat tgtatatgtt aaagtagtga agttgactgt 1380 tgtctctgga gaacatttat gagaaacaaa ttaatcaata aaacatgaca actgaggaac 1440 aaaaaaaaaa aaaggccgcc tcggccactc tgcgttgata ccactgctt 1489 <210> 61 <211> 839 <212> DNA <213> Oryzias sp. <400> 61 cattgcgtgt gtttctcgca ctccggcaac cgtctggcct gggtttccca tgattccacg 60 gtgtctgtcg cggagggagg caagactggc acggtgaaca gcctgagctc agaggcgctt 120 cctctcctgt gtgcgacttt catcactgag aacagcatag tagcagctgg tcacgactgc 180 tacccggtgc tgtttgtgta cgatggcgcc aaaggcagtc tgacatttgg cggtaagctg 240 gatgttccaa agcaagctgc ccagaaggga atcagcgcca gagagcgttt ccagaacctg 300 gatcgccgag cctcagagac tcagggcact gacaaggacc tcaacacgct ccacaagaac 360 agcatcagcc aaatctcttt gctggaggga ggaagaaacc agtgcaccaa gttctgcacc 420 accggcatgg atggaggaat gggcatctgg gatgtcaaga ctctggagtc ggctatgaag 480 gacttgaaga ttgtgtgagc tgctctgcat ttacctgatc aaaatcaaaa tgcttctgta 540 ttaagaaaac tgctgcattg ttatacaaag cttcatggct gtatttttat caaaaatgtg 600 ccactctttt aaatttacaa acgaaacagt gttttttcgt ctacacatga caaggctctg 660 taaaaagggg ctggcctcag gttggcatat ggtacttatt ctgctgcatt agtacagtca 720 cagctcagtc tgagctcaaa gcttaaagca gccattttat atacatagca atggaaatct 780 ggaatctttt tacaaagctt atgctggtca taatatgcat ttaataaata atttctgtc 839 <210> 62 <211> 703 <212> DNA <213> Oryzias sp. <400> 62 gcagtggtat caacgcagag tggccattac ggccggggac acgctgccga gctcgccgtc 60 gactgaagga gcccgccacc cgagctgagc cggaaccgtc atggccgtgt ggggcaggaa 120 ggcgctcctg ggggtcgccc ctctgtggat gtgcttcggg agagccgagg cgctggctct 180 gtccgagcct cagatctgct acgtgctgga cggcatcctg ttcctgtacg gcatcatcct 240 gaccgccctg tactgcagaa tcaagatcaa cagcgccaga gaggccaacg gagctaaagg 300 cagacagaac gtggaggagg gcatctacac gggcctgaaa cctcacgcca ccgacacata 360 cgagaccatc ggcatgaaga agtgatgcgg caggaaggaa gtccacttct ttttttctct 420 gtatttaata accgcattta ccgctctgct tttatagagc cttttcctca cacgctgttt 480 cagaatgtcc agcatgcatg caactttgaa atctgcttga caaacatgtg gatagaacca 540 gacccaatcg tttgattcat ttttgtagaa taaaaaaata aagctttgaa gcacaaactc 600 atgaataaaa gaacgcaggc agaaagattt taatttattt atcactcaaa ggagaaaaat 660 cattcagcaa attaaaatga agataaagtt agaaacctta aaa 703 <210> 63 <211> 733 <212> DNA <213> Oryzias sp. <400> 63 atactttgac gacagcaagc agtttaagcc ggagcgctgg ctgagggaga acagcaacat 60 caaccctttt gctcacgtcc cctttggaat cgggaagcgg atgtgcatcg gccggagact 120 ggcagagctg cagctgcagc tggcgatgtg caggttggtt agagactatg aaattgtggc 180 aacagataat gaaccacttg atgtgataca ctctggactt ctggttccca gcagagaact 240 gccagttgcc ttcatcaagc gataaagctt catttgaaag tcttctggat gtgacgacag 300 accgtcgatg tgtctgttgt gatgatcctg acggtccagt gactcctacc actgctgctc 360 tgtacataga aaaatatcat aattgcgcct gaaatacctc attattattt aaaagtagtt 420 ttgcaactaa gtacgactga gcaaagcacc aaatgtaatg tacgatttaa aggttttaac 480 gaggcagctg tctttcaatc ttcattatca gacgtgtaga atgtagatgt taaactatta 540 ctttatacta ttattgtttt gttgtgtttg tacatatcca tatatctata tatgtatata 600 tcagtctgtt tatctttttt agtgtttatt ggtagaatcg ctactataat taatatctta 660 ttcctgtacg ttgttgtatg tagaaacata ttttattctc tcaaataaat gcatcgatga 720 atgcatcaaa aac 733 <210> 64 <211> 443 <212> DNA <213> Oryzias sp. <400> 64 catcaacagc cagtatgtgt ggacttttgg tggaggaacc agactggatg ttggaagtga 60 taccagtcca tccctgaccg tgctcctccc ctccacgaag gagctgcagc aggggaaggc 120 cacactcatg tgtgtggcca acaagggctt cccctcagac tggactctgt cctggaaggt 180 ggtcggcagc agcaactggg aggagatcag gagccccggg gtgctgcaga aggacggcct 240 ctacagctgg agcagcaccc tgaggctctc tgcagaccag tgggagaagg tgacctgtga 300 ggccaagaga ggatcccaga ctgcattctc agagactctc aggagagacc agtgctccca 360 gtcctgaact caaacattct ggatataaat ctgttttcta tgctgttctt cttttacatt 420 ctcttctttt caaactctgt ttg 443 <210> 65 <211> 758 <212> DNA <213> Oryzias sp. <400> 65 aagcagtggt atcaacgcag agtggcatta cggccggggg gactgcgagg agtttggagg 60 agtttggagg agtttggagg agccgcgggt ggaaatgtct cagtactcag gaaagatcgt 120 cctctacgag gggaaaagct tcacggggag gaagctggag ctctgcagcg aatgcgacaa 180 cttccaggac cgcggcttca tgaaccgggt caactcggcc cgcgtggaga gcggcgcctt 240 cgtctgcttc caccacccgg acttcaaggg tcagcagtac atcctggagc acggggagta 300 ccccgaattc cagcgctgga acggccacaa tgatcacatg ggctcctgca gaccaatcag 360 gatgcacgga cagcactacc ggctggagct gtatgagggg gaccggttca gcggtccgtg 420 tgtggagctg agcgacgact gtccgtttct gcaggccaga ggtctgacca aaacctgcat 480 caactccctc agggtttatg gagatggagc gtgggtgttg tacgaggagc caaactaccg 540 cggtcggatg tacgtcgtcg agcggggaga cttcggctcg cccgcggact ggcaggcgga 600 gaaccccaac atccagtctg tgcgcagggt ggctaactac ttctgaagct agctcccatc 660 cgcctcatgc agctgacaat aaagatgcag caaacgaaca aaaaaaaana aaaaaaaaaa 720 aaggccgcct cggccactct gcgttgatac cactgctt 758 <210> 66 <211> 818 <212> DNA <213> Oryzias sp. <400> 66 ctgacggcgg accagctcgt gctgcttttg gaacttctgt tggaggaacc ggagctgagt 60 gcggcatcca tgcacgccct gtacaagatc tacaatctgc acagacaaga cgctgaggtt 120 cgacatcgct ggtgtgagct ggtggtcaaa catgctttta ctcagggcta cgcagatgtt 180 gaacattttt tactttacga tcaggccatg ggtgtgtatc tgtacgggga actgatgctc 240 caagaggacc ctgaacagca ggctctggct cgccgctgcc tctcagcagt tgcagaggaa 300 atggaccagt cagcgcgcag agtggtggag gagatgatcc tgtgactctg gaggttgcag 360 agacgttatc aggatccaga actcacctgt ttttctggga tactgggcac caaccgtcca 420 aactgttcac acaagactat tacatcttta cactgtaaat gttttaggaa ttaatttttt 480 tttttactca acgcagagag ccatgtttta gagtctgagc tgctgtgtta gtgtgagttc 540 atgtctgttt agtctaaagt catcatctgg tttttaagag tttagactga aaatacattc 600 agtgagttct tatttatttt attttttttt taaaaacaaa ctgcatctct gacccacgac 660 cgatctgaaa tggatgaatc agcactttcc aatgcgttta aataaaagtt tatttatgtt 720 gtgtaaatga tcaataatcc attcaataaa aacaaatcct acaaaaaaaa aaaaaaaaaa 780 aaggccgcct cggccactct gcgttgatac cactgctt 818 <210> 67 <211> 230 <212> DNA <213> Oryzias sp. <400> 67 taagctggtc accacttctg ctttcatgtg caatgctccc atacacaaag tgtttgatgg 60 aatcggcgag atggtgaaag aaactctgaa gctggcttag ctccagcttt ttatgttatg 120 ttttatagat ttgctgacaa attcttttct ttctatgttt ctgagtggat ctgaaccaga 180 cgtcacactt tttttaaact ttcaataaac tcttgttttg tttgaaaaat 230 <210> 68 <211> 880 <212> DNA <213> Oryzias sp. <400> 68 tcagtctggt tctagatcaa acagatcaac atcactggct ttaatggaat taacagaaga 60 aataactatc actgaagtga ataaatatgc aacaggagtt ttcatagatc taaaaaaaaa 120 agcttttgat acaataaatc atgatattct aatttataaa atgaataaac ttggagtgcg 180 aggatcagct ttagactggg agaaaagtat ttacacgaga ggcaacaatc tgtgacgatg 240 tgagaccata actcaacagt tttagacatt gtgtgtggtg tccctcaagg ctcagtgctt 300 gggccagttc tatttaacat ttacatcaac gatatagtta aaacatcaga tgaaatgaaa 360 ttcatgttat ttacagatga tacaaacatc ctgttttttg acagaaatat ccaggaactc 420 ctctagagtg accagaggac tgtataatct gaaggactgg tttgatagga acaagctgtc 480 acttcattta gacaaaatta aaatcatgtt ttttggtaaa aaaaaataca aaagagcaaa 540 ttaaaataag aatagcagga gtaacattag aaacagtgaa taatatcaga ttcctgggcg 600 tgacaataga tgacgagatt tcctggaaac agcatattag ttctatacag acaaaaatat 660 ccaaaagtat ttcagtcatg gacaaagtga aacatatttg accttcagat cacgtcagat 720 attatagaat tctctggtgt tgccgtactc aacctactgt tctgaaattt ggggaagtta 780 aaacaaaact tctttaaatc cactataaat gttacaaata gagcagtaag aatcattcat 840 aaaatgtaag ttatcgccat cacactaatc aattatttct 880 <210> 69 <211> 2119 <212> DNA <213> Oryzias sp. <400> 69 aagcagtggt atcaacgcag agtggccatt acggccgggg actgagctga aatctttcag 60 aggaacagca cgtctctctc actctgcaga gaagggaaga ttttatgttt ctgtttattt 120 atttattttt tactttaaaa aaaaaaaatg ggagattatg atgaggacac tgcatttctt 180 ggacagaccg gtccttattt ttgggtcacc ttctttcttc tgaacacggt ttttgtgtcc 240 cctactgtcta attccagatg ttaacttgac ggaagagtgg aggaacgcca tcattccagt cacagaagtg 360 gatggagaag aaattagaag tcagtgcagc aggtataagc tcgacgtagt taaaaacctc 420 tccagtgagg ggtacattcc aggaagggat gtcaacctca ccaacctgca gcaggaagcc 480 tgcttagatg gttggagcta cagcaaagaa atctaccaat ccaccatcgt cactgagtgg 540 aaccttgtgt gtgacaacca gtggaaagtt ccatttgcat cctcatctct ctttgtggga 600 tacctttttg gctccctaat atcaggacaa ctttctgaca ggtttggaag gaaaaaagtc 660 gttttcctgt ccctggcggc ccagtgcctc tccgttctcc ttcagtcttt ttcccaatca 720 tggagaatgt tctgcatcat attcctcttt gtcggagcct ctcagatttc catctatatc 780 tctgcttttg tactaggtac ggaggtgttg agcaaaacca tgcgggttct cttcacaaca 840 cttggggcat tcttgtttta ttgcatcggc tacatgactc tgccctggat tgcatttggt 900 atcagagact ggaggaccct gcttgctgtt ctgtctgcaa cttcggtcgt ttacatccca 960 ctgtggtggt tcgtctcaga gtctcctcgg tggttgatta ctcagggacg agtggttgag 1020 gccgaggcca ttgtgagaga tgctgccagg agaaataaag ttgctgctcc tcctgtgatc 1080 tttaaagagt ctgagataca ggaaatatct gctggcagga aatacaccat gcttgatatt 1140 ttgaaattta agaacatccg gtgcatcaca gtgatgtgtc tccttttgtg gatgtcaata 1200 aacattgggt actttggatt atccctgaac acctccaacc ttagtggcaa tccctttatg 1260 aactgcttcc tgtcagccat cagtgaagtt cctgcctaca tagtttccac tttgctgctt 1320 agggtttgcc caagaagaat tcttctgtca gtgttcctct tcctgggtgg aggagttctc 1380 cttctcatcc agttcatccc tgagactgtt cagcaggttg ctttggctct ggaaatgact 1440 gggaagtttg gctttaccat ggccttcagc atcgtgtaca tctacaccgc tgagctctac 1500 cccacagtgc tcaggaacgt cggcatggga gtgtgctcct ctgctgctcg cataggcagc 1560 atcacagcgc cctacatcat ctatttaggt acatacgata aagttctgcc ttatatttta 1620 atggggagtc tcaccatctc ctcctcgata gttaacttgt tcctccctga gactctccac 1680 agagatctac cagaaacagt ggagcaaatg caagaatgtc aggggccgtg caaaagagcc 1740 acaaagagga aatgtattca aaatggagcc acacacaacg cactctctca atgtgaagcc 1800 aaatctgaag gggaaactct caatctgtag ttactttatg tggcaggact taaagtctct 1860 tttttgttag aagtggtcaa acattaaaag aaaactccct tgtgattgtt atatttacat 1920 agttacataa acctaaccag ctgttttgta ggacaggagt gaaaacagtt tctctttttt 1980 ttggtaatta aatcaatgta tttgtttgtg attttaaggc aatatttcta cagctaataa 2040 ttaactgata ataattataa ataataattt caactttttt aaattgaaaa gttaaaaagg 2100 tcatttacta tttttattg 2119 <210> 70 <211> 615 <212> DNA <213> Oryzias sp. <400> 70 nacygucsam nmraskryas atstacacct tcagccactt tcctgacgac aagagtggcg 60 agtggtttgg ttatctgacc cgcgatggca gcgtggctct ggacttcatg ggaggcccct 120 tcaaagggtt cttccatgtg ccccgctgcc tgctgatgtg tgaacgcctg ctggacgatc 180 tgctgcaaac acacgacgaa tgaattctac aactgaaata aaaagaaatc aaagtgtctc 240 tctgttcaga agcactgaag ctcttcttct cacttttctg tctcatttct tctgcttttg 300 tgttgatttc tcattgattc cagcggagga gcagcgttcg acagtaagga aagactgttt 360 tcatgaagga gtttgggacg gacaacacat gctctgagct ccacctcact tgctggggcc 420 ctctcctgtc atatatgggt ggggtctgcc tgtaggatgg agtctggggg tcgggggccc 480 atctttccac cttaaatgac tagtctaaag taatttaact taaaatctaa tttaaaaaat 540 aatagaaatt acaccatatc aagttataga atttatatat tgcataaaca tattattaat 600 attaattgcc ttgcc 615 <210> 71 <211> 1067 <212> DNA <213> Oryzias sp. <400> 71 aagcagtggt atcaacgcag agtggccgag gcggcctttt tttttttact atgttttatt 60 ctgttcatgc tttggattca catgagaaaa atacgagggg atcctgaaga gaaacaggag 120 aactaggaaa gcaacctttc gttcatgctg tctcaaagaa attaaaaatt tcctgatgta 180 ggcttctga atgggatcgg ggtgctggag gggaactggg cgcttcagtt ggcagcgatg 240 gtctggtcca cccaggcacg cagcttagtg actcgggcgt acacaccagg catggaggtg 300 ctgcaggttg agcttcccca ggacacaata cccaccagag tccaggctcc gtccttctgg 360 cagaccaagg gaccgccgga gtcccccatg catgaagagg ctccggaggc accagcgcag 420 atcatcagat ctgtgatgtt gctgccccag tagctgctgc agttagcatt ggtcaggagg 480 ggcagggagg cctgctgcag cagagcgggg gtgttggcat tggagtggtg ggtcagaccc 540 cagccagagg tcatacacat catgcctcca gggaagttgt cgttggtttc agccacacac 600 acgggggaga cgcgggtgtt gagctgggcg ggggtggcca gcttgatgag cgtgatgtcg 660 ttgttgatgg tgtaggagtt gtactgaggg tgcttgaaca cccggccaac attcaacacc 720 tggatgttct cagcgttgga gttgcggtcg tgttctccaa ggatgacgta gtgagaagtc 780 ctgacgttgc agtgggctgc ggtcaccacc cagttctcat tgatgagaga gccgccacag 840 aagtggaatc cagaccaatc ctgcagggac acctgccagg gccaggagtg aggcaccgcc 900 tcctcgccat tcacaatccg agagtaaccg gtcacgatgg gggagatggc aggcctccca 960 caaccatggg cagcaccaat gaaggcgagg caagagagga tccacaggaa ggccatggct 1020 cgatcatccc cggccgtaat ggccactctg cgttgatacc actgctt 1067 <210> 72 <211> 1099 <212> DNA <213> Oryzias sp. <400> 72 gtgtggttca catcattaat gctgagatgg aaaattatga acctggggtc tacactgaac 60 gcgtgttgga gtccatcaag ctcctgtctg aaactgtcat gccccgattt gctgaacaag 120 tggaggtagc aatcgaagcg ctgagtacga accccccgca ggcctttgag gagaacgagt 180 tcattgatgc ctcccgtttg gtgtatgatg gcgtccgtga catccggaaa gccgtcctca 240 tgataaggac accagaagag ttggaggatg actcagactt tgaacaagag gactatgata 300 ctcgcagcag gaccagcgtc cagaccgaag atgaccagct tattgcagga cagagtgctc 360 gggccatcat ggctcagctt ccacaggagg agaaggccaa aatcgccgag caggtggaga 420 gcttcagaca ggagaagtgc aagctggatg cggaggtcgc caagtgggac gacaacggca 480 acgacatcat tgtgctagcc aagcagatgt gcatgatcat gatggagatg acagacttca 540 ccagaggcaa aggccccctg aagaactcct ctgatgtgat caacgccgcg aagaagatcg 600 cagaggctgg atccaggatg gacaaattgg ctcgggctgt cgccgaccag tgccctgatt 660 cagcctgcaa gcaggatctg ctggcctacc tgcagagaat cgccctgtac tgccaccagc 720 tcaacatctg cagcaaagtg aaggctgagg tgcagaatct gggtggagag ctcatcgtat 780 ctgggctgga cagcgccacg tctctaatcc aagcagccaa gaacctgatg aacgccgtgg 840 tgctgaccgt caaagcctcc tacgtagctt ccaccaagta ccagaaggtg tacgggacgg 900 ctgcggtcaa ctcgcccgtg gtatcttggc gcatgaaggc gcccgagaag aagcctctgg 960 taaagcggga gaagccggag gagtgtcaaa cgcgtgtacg gcgaggctcc cagaagaaac 1020 acatctcccc cgtccaggct ctcagcgaat tcaaggccat ggactccttc taagaggttc 1080 aaggaaaaag ctgcgaaaa 1099 <210> 73 <211> 592 <212> DNA <213> Oryzias sp. <400> 73 tacaccttca gccactttcc tgacgacaag agtggcgagt ggtttggtta tctgacccgc 60 gatggcagcg tggctctgga cttcatggga ggccccttca aagggttctt ccatgtgccc 120 cgctgcctgc tgatgtgtga acgcctgctg gacgatctgc tgcaaacaca cgacgaatga 180 attctacaac tgaaataaaa agaaatcaaa gtgtctctct gttcagaagc actgaagctc 240 ttcttctcac ttttctgtct catttcttct gcttttgtgt tgatttctca ttgattccag 300 cggaggagca gcgttcgaca gtaaggaaag actgttttca tgaaggagtt tgggacggac 360 aacacatgct ctgagctcca cctcacttgc tggggccctc tcctgtcata tatgggtggg 420 gtctgcctgt aggatggagt ctgggggtcg ggggcccatc tttccacctt aaatgactag 480 tctaaagtaa tttaacttaa aatctaattt aaaaaataat agaaattaca ccatatcaag 540 ttatagaatt tatatattgc ataaacatat tattaatatt aattgccttg cc 592 <210> 74 <211> 438 <212> DNA <213> Oryzias sp. <400> 74 tgtcttgatg gaaaaattgc ctgacaacgt tggaaaaaaa agatttctta ccccgtgctc 60 atcaacattt gtggcatttt gaacaacctg gtcatcggcg gccttgaagc tgcaaaaatg 120 atcaccgaaa aggatggctt gcccaaacta ataggaatca gggattctgg agacattacc 180 gccgaaggtt gtcaagcatc cagatcagca ggaacagtcg tatccaacat gtactattat 240 aggaaactgc acaaactgta caaatcgaaa ggctacaaaa gtggagactt tgctgacaag 300 tttgcactct agacctttct cctttaagca aagttcattc tttatcttca agattttgca 360 ccaagaccac aagtaaagca aagaaaatga ctgtgtttta ctacaacaaa tatctttgga 420 ggcggtacct gtggatcc 438 <210> 75 <211> 1282 <212> DNA <213> Oryzias sp. <400> 75 aagcagtggt atcaacgcag agtaaccatt acggccgggc agggatgaag tgaatgtgga 60 ggtggactca gccactgggc cagaactggg caccgttctt tcagagctgc gctcccagta 120 tgaagggatt gtccggaaga acaaggcgca ggccgagcag tagtaccgca agaagctgga 180 gacagtgcag aatgaggtga aggagagcaa tgaagctcta agaggagctc agggagagct 240 gactgagagg cagcgcttcc tgcagaccct ggaagtggag ctggacagtc tgcacaaaca 300 gatagcagct ctggaaggaa acctggccga gacgggccag aaatattctg gtgaaatgga 360 gcgcttgcag gtcacgctga gccagctgga agatgacctc tcccagctca ggctggacat 420 gcagcgcacc aagaccgact atgaacagct tctccgcatc aagcagaacc tggaaatgga 480 gatcgctact tacaggcgct tgctggaggg agaagaagca gccaaagaaa ttcctcctcc 540 accaaaaaaa gaaccggacg tccgcaccag gaagattgtg aaagtggtga ctcaaactat 600 ggtgaacggc aaagtggtgg atgagtctag tgaggtggag caaattgaag agtcaaagaa 660 atagaaatgg agacaccgat cgaagctgaa aaagatgttg tttttctttt tatgaagcag 720 ttaaggaaat gtattttagg aatcataaag aaaaaatatt tacatactgt acatggagca 780 ttagccttct attctattgg gaaaatatta ttcaattgag tcagttaatg tgctataagt 840 tgctgttgag tataagatgt agacacaaac aaaattacac acaagtcaca cttttgggag 900 aaatttattt aacaaaaaac atgaacagct gaattctttg tctgtgctcc actgaggctg 960 gcgcttcttc ttctgtgttg caaaaacaga taaacaccta cagtgtcttc aagagaatat 1020 caatgtatgc atgagccttt caaaaaatta aataaaaaaa aataccaaac ataggccgca 1080 cccctgacca aacgattcaa aaatgtgaat tatgctccaa aattacagtt gtttttcatt 1140 attcttgatg ggtctttagt agttgtgtat gtgtgtgtct tggtttgatg taggtttcta 1200 ttagaagaga acagagtgaa ctaataaaaa actgcatact tagaacactt ggaaaactgg 1260 taattttgtc tttactaaat aa 1282 <210> 76 <211> 733 <212> DNA <213> Oryzias sp. <400> 76 aagcagtggt atcaacgcag agtggccatt acggccgggg actaaggatt gaagatggca 60 ggtacagact ccggtacggt ctcctatgag gaattgaagc atttattggc aaacaacccc 120 aatctatttc tggttgatgt acgcaaccaa gacgagtggg atgctggaca tattccaagg 180 gccattcaca tccaattgga cacggtagag gctgcctttt caatgaaccc agaagacttc 240 agagccaggt atggagtgga gaaaccgccg ctgaattcac caaacatggt gttttactgc 300 cggaccggaa aaagatccgc acagggtttg tgcacggtca aacgcctggg atatgtcaat 360 gttcgcaatt accctggcag ctacacagag tggtctgcga gagaaggaag aggctcatcg 420 taaactgcac acaagtcttg tatttttgtc ttgcttttgt ttttttttta aattggaaaa 480 tgtttttagg caaagccatt tgagtcacct tagttggaaa agtgctacaa aattaaaatt 540 tgatttgatt tgatttgaag aaagactgat aatgctaatt gttcccattg aggacacgaa 600 gtgtcacttg taagatgagc taccaaataa aatattttgc tctttgtgct tgttgtgtta 660 cttttatatg aatataaaaa gaagaggaca aaaagatact ttgtaccagt tcagttttgt 720 gacatacatc tgg 733 <210> 77 <211> 1941 <212> DNA <213> Oryzias sp. <400> 77 aagcagtggt atcaacgcag agtggccatt acggccgggg gacatgcaag ctgaagctct 60 cccacacctg acaggaggag ctcacacaca gtcatgagga tgaggaagct cctgtgggtg 120 agtctgctgg ctctgctgct gctgcaggcc tcctgcatcc acgccaacga ttcagacgac 180 agcacagagg agcacagaaa tgctcaagac gacacaacaa aggaaaccac agaggaggcc 240 tcggcagaga cagacaaacc tgaagagcca ccgaagaagg agaaaacaac agagatagaa 300 gaagagcaag acgtcatggt tctgcacatc aacaacttcg acagagctct aagcgagaat 360 gaacatctac tggttgaatt ctatgctccc tggtgtggct actgtagaag atttgagccc 420 atttatgccg atgctgctgg gatgttgaag gaggaaggct cagccatgcg cttggccaaa 480 gtggatgcca tcgaggagaa agagctggct gaagaattta acatcgacag cttccccact 540 gtgaagctgt tcatgaatgg agaccgaaag cagcctattg aatacactgg taagaggaca 600 ccttcaggaa tagttcagtg gttgaagcgt cgctctggac ctggagctgc agacctggac 660 tctgcagact cggcagctga gttcatcaac agccacaacg tcacagttgt tggattcttt 720 gatgacctgg aaagtgaagc tgccggtgtc tttaaggaag ttgcttttga tttgacagat 780 gttgagtttg cagttacaac atctcctgaa gtctttaagg agtatgaggt gacatccaac 840 tcagtggtgc tgttcaagaa gttcgatgat ggcagagcag actttgcact gtctgaagag 900 gacaagctcg acaaacacaa cctgacaact ttcattaagg aaaacagtct ggagctgatc 960 gtctcattca accaagagac atcagacaag atcttccact ctcgtgtgcg gctgcactgc 1020 cttttcttca ttaactccac tgtgcagagt cagatgagcc tgctggagga gtacaaggct 1080 gtcgccagag agtttaaagg aaaggtgctg tacatcatga tcgacatcat gacatctgtg 1140 tcacatgtgc tgaactactt tggcgtgtta gagacggatg taccgactgt acgcatcatc 1200 aacatggaga cacaaaaaaa gttcaaaatc gactctgacc tgaccattga ctctctgaga 1260 cagctttccc aggacgtagt tgatgacact gcacagccgt acttgcggtc tgaggagatc 1320 cctgaggact gggacagggg cccagttaaa gtcctggtgg ccaagaactt tgagtctgtt 1380 gctatggacc ccaccaaaag cgtctttgtt gaattttatg ctccctggtg tggacattgc 1440 aaggagctgg ctcccatctg ggagcagctg ggtgaaaaat atgcagatca tgatgacatc 1500 atcattgcaa agatggacgc cacagccaat gaagtggaga gtgtggcagt tgatggattt 1560 ccaacattga aatttttccc agctggtggc aaagaggtaa ttagctacac tggaaacaga 1620 gatctagaaa cgttttccaa gtttcttgac aacggaggag tgctgccaga agaggaaagt 1680 gatgaggacg atgacgatga agaagaggtt aacaaagatg aagctaagcc aaatgaagga 1740 gatgacgctg atgatcagag caagcagaca gatgactctt cagagggacc aacaaacaac 1800 acatccaaag acgagctgtg atgacaattt aaactcaacc actctgtcat ttttcttaat 1860 aaaatagtat tcaagttaaa aaaaaaanaa aaaaaaaaaa aaaaaggccg cctcggccac 1920 tctgcgttga taccactgct t 1941 <210> 78 <211> 980 <212> DNA <213> Oryzias sp. <400> 78 ttgttctggc caagatggca tccgcccgga agagggctca gacggtgggt ttcagcaagt 60 ttgccgtggt caatcttcgg gatggagttc tgtgtctgtc atggcgcctc ggggacttca 120 gagggaacca tatactggag ggggtggtca cagctcagct ggtccggtgc atgaggcggc 180 cgcagggttt catcgcgtgg tcataccagg atctgaacat agaagagcca gaaattgccc 240 tcaccacacc agctactatc atccacaagc tggaaccagg aagtccgctg tacagcttgg 300 atccggacga gctcctggag gagaactttg aactggtcgt ctctttcacc tatacaggag 360 actcgacggg catgctccac cagacacgca ccacctacac accggcagac atccgctggg 420 gtcagcgctt caaggacatt ttaaaagtgg gcaagaagca ctacaaggtg gactacgctt 480 tcttcaacga gaccacatgg gtgcaagtgc ccatgatgag cgcagagtcc tttaacagga 540 agcgacacct tgaagacggc cggctacacg tgtcatcggt caaaagaaaa ggcggcactg 600 aacacgtgac agtagaaatc actgaagaac cagtgttgca gacatttttt gtaacatttt 660 gagtttgagg agtcctattc tttaaaatgt tgccccatgt gagaagttcc catttcccta 720 gaactttcat tttgcaaacc gatgttattt tcctcagatt tcacatcatt gttcacacac 780 acacacaaaa aaagaataaa cactgtagaa cacttgtatg tattcaaaaa tggttcaaaa 840 tgtacttttt agaggctttt atctgaaaca tgttatcttt tgacatggca taacataaag 900 aaatgtggat cataccatat tgtgtaggag tggggggaca acttcagact tgtacatttt 960 tctatttaga tttcagatca 980 <210> 79 <211> 431 <212> DNA <213> Oryzias sp. <400> 79 aagcagtggt atcaacgcag agtggccatt acggcctggg gaggtccacc tggacgacct 60 gaaccgcagg atctgctaca gttctcatgg agcctacgcc cagagcaagc tggctctggt 120 cctcttcacc taccacctgc aggagcagct cagcgccggc ggcttccccg tcaccgtcaa 180 cgccgtggac cccggcatgg tggacacggc gctctacgat aacctgtgga ccctggcgca 240 ggtgctgaag aggccggtgg ccaaggttct attcaggact ccggcagagg gcgcctccat 300 ctccgtgtac ggcgccgcgg ccgcagagct ggagggcgtg ggcggctgct acctgtacaa 360 cggtctgaag acccggtcct cagagctgtc ctacgactgc cagctgcagg agcagctgtg 420 gaagaagagc t 431 <210> 80 <211> 1876 <212> DNA <213> Oryzias sp. <400> 80 aagcagtggt atcaacgcag agtggccatt acggccgggg actctttcat gagccatcat 60 aaccaacagc tgtccgtcta gaatggggaa agttcatcag gaggatagca atgtcaaaga 120 gaagaacagt gagttgagct tggtgagggt caaaggtaca tggaagagca aaacgggctt 180 gaagatcaag gacgctgttg actgtgtttc tgctgctaaa atatatgaaa gcatctcaga 240 gcaaggcact acttggacag tggtcagccc catcaccttt acctacaagg tcactgagac 300 tcatagttta ctggatccaa gcaatgacac tcttctactg ggccacatca ccgacccaca 360 gcaaatggag gacattaaac gctcaaacaa gcccatgagg cgatttgtgg tcgtcgatca 420 ggaagtctac aaaatctatg gtcccaagtt aactgaatac ttagaagcta atgatgtggt 480 ttacaaagtc ttggctcttc caaccacaga ggagaataaa tccatggaaa ctgcactgaa 540 aatcctggaa gaagtgaaca acttttccct ggaccggcgc acggagcccg tcattgctat 600 tggtggaggg gtttgcttag acatagtggg tctggctgcg tcgctgtaca ggcggcgcac 660 tccgtacatc cgggtcccaa ccaccctcct gtcctacatc gacgccagcg tgggggcgaa 720 gacgggcgtc aattttgcaa actgcaagaa caagctgggg gcctacatcc caccagctgc 780 tgccttcctg gacctgtcct tcatgcagag tgtcccgagg agacacatct ccaacggtct 840 ggctgagatg ttgaagatgg ctctgatgaa gcacaagggc ctctttgagc tcctggagga 900 acacggctgt atgctcttag acaccaagtt tcagactgga aacaatgtat atggacacaa 960 cagtgtgaag gttgcatctc agacaacccg aatagcaatt ctaaccatgc tggaggagct 1020 ggctccaaat ctttgggagg atgacctgaa cagactcgtt gactttggtc atcttataag 1080 tccagcacta gaaatgaaag ttctcccgtc cctgctgcac ggggaggcgg tcaacatcga 1140 catgtcctac atgacttacg tgtctcacga gagcggcctg ctgacggagg aggagaagca 1200 gaggatcatc agctgcatgg tggggctgga gcttcccgtc tggcacgagg agttcaccat 1260 ggagctggtc cagaagtccc tgcaggacag actgaaacat tccggcggcc tggtccgaat 1320 gcctctacct gttggtctgg gacaagcaga aatatttaac aacatttcat acgacatcct 1380 ccagaaagct tttatcaagt ggtgtgaaga gctgagcgtc tcctctgata gcacctgtga 1440 cgcttgagct tcagcttttt aaagaaacgg gtttttattt cagctactac gtttggtgtt 1500 tggaagtgta atgttgttca agcagtttgc tgtggttaaa gtctgatgtc caacaatgat 1560 aaaatgagat ggttccactt ccactgatgg agactgtaaa tatcctttct gaaaaacaca 1620 ttatagcagc tacttctgtg agtcatgaag acaaatgatg acagcatttc ttcactttgt 1680 tttacagttt cagaatagct tgaatggaat aatcaagaaa tgtttttaag tggtttaaca 1740 ctaatttaca ttaaacagtt gaagtgttat ttgggggaaa aaatggatta aaggaaccag 1800 aagttagttt ggtacgttct tgtttgtgct taaaaaatgg aaacaaactc cattaaaagt 1860 tttttattaa cagtac 1876 <210> 81 <211> 685 <212> DNA <213> Oryzias sp. <400> 81 aagcagtggt atcaacgcag agtggccatt acggccgggg agaaacagat tcagcatcag 60 acggtagaag actcagatcg tcaaaatgaa gattctggtg tttctgtgtt tggtgggatt 120 gagttttgac ggcgctactg cagtgacgca ttctctgaag tatttctaca ccgcttcctc 180 tcacgtccag aacttcccag agtttgttgc tgtcggtttg gttgatgatg ttcagatgat 240 tcactatgac agcaacacca gagtggcggt tcctaaacag aactggatga aggagggtgt 300 ggatcaacag tactgggaga gtcaaactgg aggccttctg ggtgctcagc agagcttcaa 360 agccaacatt gaaatagcaa agcagcgctt caaccaaact ggaggtgttc acgtgttcca 420 gaacatgtac ggatgtgagt gggatgatga gactgaagag gtcaaaggtt attatcagtt 480 tggttatgat ggagaagact tcattaatct tgacctgaag gatgactcat ggactgctcc 540 aaaacagcag gctgtcatca ctaaacagaa gtgggataat gacaaagctg acatggcata 600 cacaaagaac tacttgtctc tggagtgtcc cgagtggctg aagaaatact tgaaatatgg 660 aagttctttg aaaagaacag tgtct 685 <210> 82 <211> 994 <212> DNA <213> Oryzias sp. <400> 82 aagcagtggt atcaacgcag agtgcccatt acggccgggg tttcatcagt tttttcagaa 60 agagaagaga gctgggctcc tgatctcagc tgagtcatgt ctggactcaa tgcgtccctc 120 ggatacttcc tggctgtcgt gatttttgca actttgctgc gaatgctttt caacaaatgg 180 ccacgtctcc atttcatggc agagtttgcc tcttctttca tgctggtggc atgttggctg 240 gaggttcaga ccatagtgga ggtgggcgag tgggctgggg gtctgggtcc tgatgtgact 300 gccaccatgc tgcttgtggt gctgctgact catggtgtga tcagtggggg ggcatcgggg 360 aaccccagct tggctgtgca gaagttcctg cagctggagg acagccccct gcccaccctg 420 ctgacggtgg cagcccagtt tctggggtct cacgcgggtt tccttgcagc agggttttac 480 tggaggctgg agctgacgga catgcacatg atcaaaaacc tgatgtccag tgagtgcagt 540 acttccctgc aggtccctgt gctgcagggc ttttccactg agttgctctg tgcgatcgtc 600 tttcatctcc tctgcctgag cctgaagcgc agatctgctc tgattggcct tccgctggtg 660 gctctcctcc gaacattgct gtctcattct gcccgacgct acacttcagc ttacatgaac 720 ccatctttgg cgtacggcct caccttccac tgtcctggat tcaccttcac agagtatgca 780 gtggtctact ggctgagctc tctcacaggc atgactttgg ctcttctgct gtacatgggc 840 cacattccca ggatgtttac caaaaacctg ctgtatctcc agaagacacg cttcagagtg 900 cccaaaggag acaaagcaga gaagaaaaag atgtgaacga tggagaaaac acactcaatc 960 attaggatgc atttatttta gacaaagtgt aggg 994 <210> 83 <211> 865 <212> DNA <213> Oryzias sp. <400> 83 cgttgacaag ctaatattaa taacagtgag aatcagttgc cctatgctgg tgaagattag 60 tttaaaaaac gggggggac ggcatccaga ggagagcctg atggtttcat gtggttgaca 120 atgtcttcaa gaaaagacag agtcacaggc tcgaacgact ggagcacaga tcgaggaggg 180 ataaaatcag atggatcaaa gccgggtgct agaataacag atctgatgtt agcaaccttc 240 tgagtaaaaa agtttaaaaa ttcatcacac atttcagaag atgcttcctg gcagtcagac 300 tgtggggtgt taagaacaga ggtgacagtt ttaaaaagca cacgtggatt gtggctgtta 360 gtctcgataa tattggacaa atatgcttca cgtgattttt tgacagtgat cagataatga 420 tgccaacagt cctttaaaat ctggagtgaa acctgtagct tgtccttttt ccacctccgc 480 tccgctctac gagtctccct ccttgcctgc cgggtgctct cattaaacca gggctccggt 540 ttggccctgg gtttcatggt tttaaatgga gcaacagaat ccagaacatc caggcaggag 600 gagtgaagaa tacgtttatg tttatatatg tttatttttc tacatctgct aaacctaagt 660 cgtatgctct gtaaaaggta ctttgttttc aaagaatgtc aagcttcatg gctttaatct 720 tgtcgatcat tttttgttct tttaattatt ttcttttatt ttttgtgaaa acaagggact 780 tagtatgtta agacactaca gattttaaca ttggggtgta atgcctctgt attgaaaaaa 840 aatgtttagt ctttctttgt aacta 865 <210> 84 <211> 2216 <212> DNA <213> Oryzias sp. <400> 84 ttacggccgg ggatcattta acacagcaaa gaacacagaa aggagaatag ttgagagaat 60 ctgcgcagaa aggaagaggc ttctttcagc tgagactacg cgaaaggcca tcgttttact 120 gattaagctg ttgtgggcgg taatgctggt agtcggcgtc gtgttcgccg aggaggagga caagaaggac 240 agcgtgggaa ctgtgattgg aattgacttg gggactacct actcttgtgt tggagtcttc 300 aagaatggcc gtgtggagat cattgctaac gaccagggta accgcatcac tccctcctat 360 gtggccttca caagtgaggg agagcgtctg attggtgatg ctgccaagaa ccagctgacc 420 tccaacccag agaacacagt ctttgatgct aagaggctga tcgggcgtac ttggggtgac 480 tcctctgttc agcaggacat caagtatttt cccttcaagg ttattgagaa gaaaagcaag 540 ccccatattc agattgacat tggtggtggt caaatgaaga cttttgcccc tgaggagatc 600 tctgctatgg ttttgaccaa gatgaaggag actgctgagg cctatctggg aaaaaaggtc 660 actcatgccg tggtcactgt tcctgcatac ttcaatgatg ctcagcgcca ggccaccaag 720 gatgctggaa ccattgctgg cttgaatgtg atgaggatca tcaatgagcc aactgctgct 780 gcaatcgctt atggtctgga caagaaagac ggcgagaaga acatccttgt gttcgacctt 840 ggtggcggca ccttcgacgt ctcccttttg accattgaca acggtgtctt tgaggtggtg 900 gccaccaacg gtgacactca tctgggaggt gaagacttcg accagcgcgt catggagcac 960 ttcatcaagc tgtataagaa gaagaccggc aaagatgtgc gcaaagataa ccgggctgtg 1020 cagaaactgc gccgtgaagt tgagaaagcg aagagggctc tgtctgctca acatcaggcc 1080 cgcattgaga tcgagtcctt ctttgaggga gaagacttct ctgagactct cacccgtgcc 1140 aaatttgagg agctgaacat ggatctgttc cgttctacca tgaagcctgt ccagaaggtg 1200 cttgaggatg ctgacctgaa gaagtctgaa attgacgaaa ttgtgctggt tggaggctcc 1260 acccgtattc ccaaaatcca gcagcttgtc aaggagttct tcaatggaaa agaaccctct 1320 agaggcatca accctgatga agctgttgca tacggagctg ctgtgcaagc tggagtgctc 1380 tctggagaag aagacactgg tgatgtggtt ctcctggatg tgtgccccct gactcttgga 1440 attgagacag ttggaggagt aatgaccaaa ttgatcccca gaaacactgt ggtgccaacc 1500 aagaaatctc agatcttttc taccgcctct gataaccagc caactgtcac cattaaagtt 1560 tatgaaggtg aacgtcctct gacaaaagac aaccatctgc tgggcacttt cgacctgacc 1620 ggcatccccc ctgcacctcg cggcgttcca cagattgaag tcaccttcga gattgacgtg 1680 aacggcattc ttagggtcac tgctgaagac aagggcactg gaaacaagaa caagatcaca 1740 atcacaaacg accagaaccg tctgacgccc gaagacatcg agcgaatggt gaacgacgct 1800 gagcgcttcg ctgacgaaga caagaagctg aaggagagga tcgacgctcg caatgagttg 1860 gagagctatg cctattctct gaagaaccag attggtgaca aggagaagct tggtggcaaa 1920 ctctcagatg aagacaaaga ggccattgag aaagcagtgg aggagaagat tgagtggatg 1980 gagtcacatc aagatgctga gacggaagat ttccaggcca agaagaaaga actagaagag 2040 gtggttcagc caattattag caagctatat ggaagtgcag gtggtccacc accagagggc 2100 gctgagagtg agggagagaa ggatgaattg tagggaggtt tggcctcatt tgttgcctct 2160 tgccaccatt acagtggctt ctgcttatga aaataagact gatgggactc gatagt 2216 <210> 85 <211> 241 <212> DNA <213> Oryzias sp. <400> 85 attctagctc tcagtttctg ttgtctagat cttgctctca tctcctcacg cgtcctcagg 60 tgtctgatcg tctacccctg gactcagagg tacttcggca gttttggaaa cctctacaat 120 gccgctgcca tcagctcaac ccaaaggtcg cggcccacgg aaaggtggtc ctctcgggtc 180 tgcagttggc cgtgaataac atggatgaca tcaagcagac ttacgctgat ctgagcgtgc 240 t 241 <210> 86 <211> 900 <212> DNA <213> Oryzias sp. <400> 86 aagcagtggt atcaacgcag agttggccat tacggccggg gacgactggg cgaaaatggc 60 ggagtatctg gcatccattt tcggcacaga gaaagacaag gtcaactgtt ccttctattt 120 caaaattgga gcttgcagac atggagaccg ctgctcgaga ttgcataaca aaccgacctt 180 tagtcagacc atcctgatcc agaacatcta ccgtaatcct caaaacagcg ctcaaacggc 240 tgatgcttct cgtgccgtca gcgatgtgga aatgcaggaa cattacgatg agttctttga 300 ggaagtcttc acagagatgg aggagaagta cggagaggtg gaggagatga acgtgtgcga 360 taacttgggc gaccacctcg tcggcaacgt ctacgtgaag ttccgccgcg aggaggacgc 420 ggagaaggcg gtcatggacc tgaacaaccg ctggttcaac ggccagccca tccatgcaga 480 gctgtcgccc gtcaccgact tcagggaagc ctgctgccgt cagtatgaaa tgggagagtg 540 cacccgcggg ggcttctgca acttcatgca tctgaaaccc atatccaggg aacttcggag 600 ggagctgtac ggccgccgca ggaaaaggca ccgctctcgc tcacgctccc gcgaacggcg 660 ctcccgctcc agggatcgac gacgggaccg cgagaggcgg aggtcgaggg accgagaacg 720 ctccggacgc ttctagagga aggccggata gatccaccga acccctccct cctaacacga 780 cagtgacaac gttttcattt gtttttcttg gtgaattgtt ggacgtgttt gtttctttta 840 ttcccttttt taagacaagc ttgtgtcgct ttctcaataa aacagatttg caactctaag 900                                                                          900 <210> 87 <211> 1389 <212> DNA <213> Oryzias sp. <400> 87 ggagacctgg ggaaatatga ccaggacaac ttcctgtaca tcacagggag gatcaaagag 60 ctgatcatca ccgccggcgg cgagaacatc cctcctgtgc caattgagga cgcagtgaag 120 agcgagcttc ccatcatcag caacgccatg ctgattggag acaagctcaa gttcctctcc 180 atgctcctca ctcttaaatg tgtcatggat gataacggcg agccaaccaa tgagctgagc 240 tcagaggctt tggacttctg ccggcatcac ggagttaatg ccaccaaggt gtcggagatc 300 acagccaata aggagcctgc tatctacaaa gctattcagg aaggcataga tcgcgtcaac 360 gcaagatcaa cgtccaacgc tcagaggatc cagaagtttg tcgtgctgga gcgagatttc 420 tccgtcgttg gaggagagct gggacccacc ttgaaactgc ggagacccat tgttgtgaaa 480 atgtaccagg aaaaaataga tgagatatac tcaggagctg cagaaaaaca gtagactgca 540 ctgcactgca ctgcactgtt cggagagccg aggcgctcat gggaaatgtg gccagaaaca 600 gaggagaagt ttgtcttgcc aaaagctgtg aggatgtctc tctttgcttt gaatgtaaat 660 ctacgactta cagtctgaca ctggcagcac agaaaagatc gcaaagcaga gcaaattttt 720 ttattttttc ccatgcgaaa cacggaggtg ggagtttcat ggcttctaaa gaaaaaagga 780 cgggacggtg caactaaaac gagttcaaat gtttacttag aagaaaagca ttcacaacca 840 ttccagttta tgttttctgg tgaagtacat ttgaatgcag cccaatgttt aaatctttca 900 tcacatggtc cccatgacaa cctggagcaa agaaacgaat gtcagctgtc acaacaagtg 960 caatgaagaa ttatcctgta tgtcggaaac gtttttttga aatatttatt tagtggtaga 1020 ttcgtcttca taaagtgaaa aaaaggctga atgttgacat gtactttatt ttgtttaagt 1080 catcacattt ttgtctgtat cagcacaaac aatgcatatc aaggggtaca ctgtctgtga 1140 aactcataaa tcatgtcaaa gcaatgaata agcaaaaata ttcatcaacg gataattgct 1200 gccactttaa ataaatgtaa agaatttttc tacgtataaa tctgattcta aatcgatttt 1260 taagattgtt ttaatcatat atatttggtt atttcaacta tttaaaaaat gattttaatt 1320 gtgttcttta aaaatgagaa aaaatgattg tgtttttaaa gaaattaaat aaatgaagtt 1380 tactaggtt 1389 <210> 88 <211> 674 <212> DNA <213> Oryzias sp. <400> 88 aagcagtggt atcaacgcag agtggccatt acggccgggg gacagttacg tcccagcaga 60 atcctttcca acactcgagt cagaatgtgg ggtctgctcg ccaccgtggc ctcgctggcc 120 gtgctgtctc tgggggtttg tgaggagaag gtctttctgc agcgagcggg cacggcctac 180 aacagcagaa agtaccgatc tgagctgaac gtgaccaacg gagagcgctt cggaacctgg 240 acctggcccg agatgtgtcc ggagaactac tttgctgttg gattcagtgt tcgggtggag 300 tcaaaacagt acggagggga cgacaccgcc ctgaacggga tccgcctcat ctgctccaaa 360 gacgagaacc ggaacttcct gtattacgtc gaatcgcaca ctggacactt cggtgagtgg 420 tctcaccctc agtactgccc cggcggcgtg ctgacctcct tccagctgcg cgtggagtcc 480 taccgcgggc ctctgggaga cgacacggcc gccaacaaca tcaagttccg ctgcagcagc 540 aaccccacgc tggaggctcc cggcatgagc tggggggata cggctactgg agccaggact 600 gtctgggggg agggatctgc ggcatcgaga ccaagatgga ggagtaccag gttggcctgg 660 acgacaccag cctc 674 <210> 89 <211> 1275 <212> DNA <213> Oryzias sp. <400> 89 gaggtcggct gcttactcta aaagctaaac acaagataaa ccccgtacgt gcaaggatta 60 aagctcacac tttgtgaaat aaacattcat tttaaagcta gcagaatgga aaagccgcac 120 attgtcacgt gtactgcccc tgtgaatata gctgttgtta agtactgggg aaagagaaac 180 gaagaattga ttttaccaat caactcatct ttgagcgtca cgttgcatca agaccagtta 240 aaaacaacga caacagtagc aaccagcaaa tcgtttaagg aggatcgaat atggctcaat 300 ggcaaagagg aggatgtcac ccatccaaga cttcagtcat gtctcagaga aatccgacga 360 ctggcacgaa agaggcataa caatggagac accacttcaa cagacttgac tggtctttct 420 cagaaagtcc acatttgctc tgtaaacaat tttcccaccg ctgccggact cgcctcgtct 480 gcagcaggct ttgcatgtct agtttacagt cttgctcagg catttggtgt cgaaggagag 540 ctgtcaggta tcgctcgtca aggttctggc agcgcctgtc gcagcatgta tggagggttt 600 gttcagtgga tcatgggaaa ccaggaggat ggaaaggaca gtttagccca gcaggtggag 660 ccagagagtc actggcctga actcagaatc cttgtacttg tggtcagcgc tgagaggaag 720 ccagtcggca gcacttctgg gatgcaaact agcgtacaaa caagttgcct actaaagcat 780 cgtgcagagt cggttgttcc tcaccggatg gaggagatga tcaaagcggt gcgaggaaaa 840 gacttcgctg cgtttgctga attgaccatg aaggacagca accagtttca tgccacctgc 900 ttggacacat accctccaat cttctacctg aacagagtct ctcaacaggt catccacctg 960 gtgcatcgat acaacagaca ttatggggag acgcgggtgg cttacagctt tgacgccggg 1020 ccaaacgctg ttattttcac tttacaacag catgttccag agtttgtcca catggtccag 1080 catttcttcc caccagaaac caatggagaa gacttcatca aaggtcttcc agtcaagcat 1140 acccccgtct ctgagcagct gaagcagagt gttggtttag agcccatgcc aaagggaata 1200 aactacatta ttagcactaa ggctggtcca ggcccacggg ttgtgaagga tccctctcta 1260 cacctgctgt cacct 1275 <210> 90 <211> 1411 <212> DNA <213> Oryzias sp. <400> 90 aagcagtggt atcaacgcag agtggccatt acggccgggc agttgccaga taatgttatg 60 gagaatcttc ctccagctca agccaatttg gaatacttca catttaaaga acaggaagtt 120 agcgtttcga taggcacccc tccattgacg agaactattt ccttgaccaa aggactacgc 180 aaactcttct tattccctca gacatcaatg aagaatgggt gctgattgag gatttaacct 240 ccaagccaga agagggaaac aatgcaatcc gatttgtgaa cggactggcc ggagacctca 300 acgtctcaac ttctgcagtg gactttggaa tcattgagcc attcatgtct tcaaactact 360 cgctgttgag aaatggacaa cacaccttca gcttgatgag tggatcacag tcatgtgaat 420 acaccagaaa atttggattt gggagttcat acactttctt cattcccagc actttcactt 480 tcggaccaaa ttgtcaggaa tctttcacag aagttgagga aataaagccc aacacggttc 540 acatggccct ccagatccct cagtactttc taatcactgc aggtgaagtg atgttttccg 600 tcaccgggtt ggagttctcg tactcacagg cccccagtaa catgaaagca gtgctgcagg 660 cgggctggct gctaacggtt gccatcggta actttatcgt cctgattgtt gcagagatcg 720 caaagcttcc taaaaagtgg gcagagttct tcctttttgc atccctgctg gtggcggtct 780 gcgtcatctt ctccatcatg gcttacttct acacttacat cgaccccacg gagatcgaag 840 ctcagttcaa ggagaagaag gaagacagcg aggaggagga ggaaaaagat gagatgaaga 900 aaactgaagt gcagatgatg aaaaaagact cgattaaaag taatgacagt gacgacggat 960 tcaaacaatc aaaaatgtaa aaagtagaac cccaccatcc aaaatctgca ataatgtcat 1020 agtaccggta atatatttgt ttaaactatt tttaacttta ataaacagaa gcatcatgag 1080 tgacgcacac ccacaatcag ttttcagagc tgatattttt aaagattaaa aaaaactaag 1140 gttgtccatt tattttattt ctgaatggaa tatttgtgta ttcatactaa ggtgtgttga 1200 agaaagcaat taattttttt atccaagttg tggtactttt tgtacattaa gtttacttca 1260 gttactagtc ctgcatcccc gctgttttaa aattaatttg tcataaagtt tcttcaaatt 1320 ccttgagtag aatatgtatc ttagtctttc tttgctttcc ccctaatcct tcctgactgc 1380 gtccacaggc ctcatcagcc cctgaatcag c 1411 <210> 91 <211> 638 <212> DNA <213> Oryzias sp. <400> 91 atttgatcca ctggattcta aaccacaaat atctgatcca ctggaatcta aacgtcaaat 60 atctgatcca ctggaatcta aaccacaaat atctgatcca ctgaccacca gtgaacacca 120 gtgaacacct ttttgcaaag ttgttgacat ttttgcaaaa tgtcaacaac gcctcttttt 180 tctacgtaaa cttcgtaaac tccaaattca tccgtccatc ctgacaacct tttacaaatg 240 tttcattgaa tccatacttt cattcaacat tgtcgcttgg tttggttcac tcagtcttgg 300 caatcgtaat gcaatcaaca gaataattaa aatcagcagt aaaattgtag gccaagaact 360 ggagtcactc atgacaattt tcaataaaaa agctaaacgt aaaggttcac acatcgccac 420 agacgccaca cacattcttc acacccatta cagcttgttg ccctcaggtc gcagactcag 480 atctctgcct ttcaaaacaa agagagctat gtccagtttt gtaccaactt caattcgcct 540 gctaaacagc taaagttgga aactctactt tagggagtct gcatcttaaa agggtatgtg 600 tgttgtatgt atgctactgt ttttattggt agtatttg 638 <210> 92 <211> 1373 <212> DNA <213> Oryzias sp. <400> 92 gtgtgttcag ggacgtgttt gtcgtctctc ctccgcattc ctgctcattc acagctcagg 60 cgtgtaactt tacacaacca actgactttc cagcatgctc cacttccagc cagcgccggg 120 tcagttcggc cccgaggtcc gagatccggg tctgtgggtg tggagggtgg agaagatgaa 180 ggcggtgccg ctggaccctt cccgaggtgg ggtctttcta caacggggac tcgtatttgg 240 tgctgaaaaa ccacggcgag cagggagcag acctccacat gtggctagga gagaagtcgt 300 ctcgcgatga gcaggttgca tgtgccatgc tggccaccca gctagacaac tttcttggcg 360 gtgaccccgt tcagcaccgg caggttcagg gctttgagac gccggagttc atggtcctct 420 tccccagagg agtcagctac aaggatggag gggtggagtc gggctttcgg aggactcagg 480 ggtctggacc ggtgcacagg ttgtaccaga tcaaggggaa acgcaacatc cgtgccaagg 540 aggtggagct gagctggagc agcttcaaca agggcgactg cttcatcctt gacctcggag 600 agattatcgt gtcgtggatc ggatcgcagg ccaacatctt cgagaaacaa aaggtgcgtg 660 aaatagcatc gttgatccgt gacacggaca gacacgggaa agccaggatc gtggacatca 720 ccgagggcga ggaaccggag gagatgctta aggtcctggg gcagaagccg gagctagcag 780 agagcacgcc agaagaagac agcaaagcgg acgcttccaa caccgctgct ctttacaagg 840 tgtccgatgc aaccggttcc atgacaatga ccaaaatctc agagaagagc ccgtttgcca 900 aggatctgct tgtgcgcgat gactgcttca tcctggacaa cggagccaac gggaaaatct 960 tcgtctggaa agggaacgga gcaaacgccg aggagaagag ggtggccctg cagatggccg 1020 acagcttcat caagcaaatg cagtacaaca tgatgaagac gcaggtggag attctgccac 1080 aagggaagga gaccatcatc ttcaagcagt tcttcaagaa ctggaactga agaggcagaa 1140 caatctccat gtcatttttt ttatacacat ttccacagaa gcagctctct ctggatttca 1200 gtggaatcgg agacgtgatg ccacttttgt aaaaattccc aaaattgttt catttggtga 1260 aattttaaag tatcatagga gttatttgtt aatatgcaga acaaaaactg tatagaggag 1320 caagatatta aacagtggtg caaatctact gacataataa accctggaaa gtt 1373 <210> 93 <211> 405 <212> DNA <213> Oryzias sp. <400> 93 gcaacgtggc gatgtacgac gccaccagcg ggaagctgga ggaggagctg cagggggtcc 60 acagcgagga gatcactgac ctcagatttg atattaatgg ccggtttttg gtgagcagcg 120 gcgacaaggc tatccgtgtg tttcacaatg cgccgggtta tcgggcggcg attagagaca 180 tgaatgacat gctcaaaaag gctcagaacg aagccatgaa gcagagactg cagcagcaga 240 tcaaagaggc tcagagcgcc ctggacttgg cactcgctgc tcctgctaac tgaaaagact 300 gtgaccctgt ttgatgtaac acttgtgctg taaggtgatg tggttgaaaa aaaaaggact 360 ttgcatgaga ttttaattta aaaaaatgct ctttttatgg attga 405 <210> 94 <211> 712 <212> DNA <213> Oryzias sp. <400> 94 attctagctg tcatccaggg aaagtccacc atgtcccagt ccaggagcca ccacctgtct 60 cccatgtacc tgtctgctgc catgctgctg gctgtgctca gctggcagta catcagcacc 120 gctgccgccc aggcgcaggg gcacgggggg acgtgtaggg gggcagtttc tcactcgtcc 180 tcacagaggt tcacagactg aagagcagac ctctgctggt ccatggaaat gcactaaatt 240 taaggattca ctatgactgt gaacaaactg gacactttga ccctagtggg gtgtattaat 300 ctggtcacac atggcttgta gatcacgatg gcaatgaaga aacttcactc agctttaggt 360 ttctcctcct ttatggcaaa tcttctctgg tttcatttct ctgatcagaa gtcagcaaac 420 ctaaccatct ttttacagag tcccagagat gtttttcaac actttttgca atccactcga 480 tctccgtaga ccacatgaga gtaaactgtg caataaacta gtttttatca agataaagag 540 gaaaacggag cgatgaactt aagttaaagt ctgttagcta agtgtcctta agtcggcttt 600 tgtgagtagt tcagcgtaaa tgtgtacaga gccaaaggaa agctgcaaac attgctatgt 660 aatattttaa tttgtacata tttgaaataa gaaataaaag tgattaagtt tt 712 <210> 95 <211> 1400 <212> DNA <213> Oryzias sp. <400> 95 aagcagtggt atcaacgcag agtggccatt acggccgggg gcactctact ggtcaacatg 60 agggggctgc tggcgttttc cctgctgttt gtagctgttc taagcaagga gacttttgtg 120 gggcaacaag tccttcgcat cgttccgaag gatgaagtcc aacaatctca gctcagaaat 180 ctggaggaga tgatggagtc cgagctggac ttctggagag gggtcacccg catgttcacc 240 cctgtggatg tcagagttcc ctcccacgcc ctgcagtttg tcaaagactt tctggggagt 300 cacaacattg agtactccgt cataattaga gatctgcagg cactgctgga tgaggagcag 360 gaggaaatgg atgcagcagc tctttctgga agtcccagga acactgacag cttcgactac 420 gcaaactacc acagcatcag cgagctttac agttttcaag acatgctggt ggctgagaac 480 cccaacctgg tcagcaagtt ggtgattggt cagagctacg agggccgtcc cctgaatgtg 540 ctcaagttca gcactggtgg atccaaccgc cctgccatct ggatcgacac tggaatccac 600 tctagggaat gggtcactca ggccagtggc acctggtttg caaagaagat tgtgaaggat 660 tatggaactg accctgctct cactgccatc ctcaacaaca tggacatctt cctggagaca 720 gtgaccaacc ctgatggata cgcatacact cacagctcta accgtatgtg gcgtaagacc 780 agaaagccca accctggatc cagctgcgtt ggagttgatc ccaacaggaa ctgggatgcc 840 ggcttcggag agcccggtgc cagcagcagc ccctgctcag agacctaccg tggacccagt 900 cctaattctg agtctgaggt caaggctatt gtggactttg tggtcgccca taaaaacatc 960 aaggccttca tctccatcca ctcctactct cagatgctct tgtaccctta tggctacacc 1020 tccacaccgg ccaaggacga ggctgagctg catagcctgg ctcagaaagc catcactgac 1080 ctggcttcct tgtatggaac tcgctaccgc tacggcagca ttatcgacac tatctaccag 1140 gcaagtggaa gtactgttga ctggacgtac gagcagggca tcaagtactc atacaccttt 1200 gagctgagag acaccggcta ccacggtttc ctgctgcccg ccaaacagat tgttcccacc 1260 gcagaggaga cttggctggc tctgatggca atcatggagc acacctacaa gaacccgtac 1320 taagtccaga actcccttca gggccccaca gcgctcaccg ttggggtgta atctgtataa 1380 ataaaacatg aatctttacc 1400 <210> 96 <211> 2112 <212> DNA <213> Oryzias sp. <400> 96 aagcagtggt atcaacgcag agtggccatt acggccgggg agaatgaggg atgcaaagcg 60 gctcgtgggc tgcgcacgtg tagcgcacat ttagaggttg ctttaggaaa acgttatctg 120 gttcttggat cagaagttta ttttagaata gagggactgt gaacaatttc tcggtttggg 180 cgaaactctt gaccggagaa gattcacgac taatgagtaa aatgatgaag tacatcctgg 240 tgactggtgg ggtcatctcc ggcatcggca aaggcatcat tgcgagcagt gtggggacca 300 tcctcaagtc gtgcggtttg catgtgacgg ccatcaaaat cgacccgtac atcaacatcg 360 acgcagggac tttttcaccc tacgagcacg gtgaggtgtt tgtgctcgac gacggcgggg 420 aggtggactt ggatttgggg aactatgagc gcttcctcga tatccgcctg accagagaca 480 acaacctgac caccgggaag atttatcagt cggtcattaa caaagagagg aggggagatt 540 acctgggcaa aaccgttcaa gtggtgccac acatcacaga tgccatccag gaatgggtgg 600 tgaagcaggc cagagtgccg gtggatgatg atcaagtaga acctcaagtg tgtgtgattg 660 agctgggggg cacggtggga gacatcgaga gcatgccctt catcgaggct ttccgacagt 720 tccagttcaa agtgaaaaga gagaacttct gcaacatcca cgtcagcctg atcccacagc 780 ccagcgctac aggggagcag aagaccaaac ccacgcagaa cagcgtcagg gagctgcgag 840 gcctgggcct gtctcctgac ctgatcatgt gccgctgttc tactgttctg gagacttcag 900 tcaaagagaa gatctccatg ttctgccacg tggagcctga gcaggtcatc tgtgtccacg 960 acgtgtcgtc catctacaga gtgcccttac tgctggagga tcagggtgtg gtgagctttc 1020 tgagcaggag actcaacatg cccatcgagg ccaagcccag gaagatgctg accaagtgga 1080 aggagatgtc cgacaggtcg gaccggctgc tggagcagtg ctccatcgcg ctggtgggga 1140 agtacaccaa gttctcagac tcgtacgcct cggtcattaa ggctctggag cactctgcac 1200 tggccatcag tcacaagctg gaggtgaagt acatagactc ggcgtgtttg gagccagcca 1260 ccctgcagga ggagccggtg aaataccacg aggcgtggca gaagctctgc agcgccgatg 1320 gaatcctggt tccaggaggg ttcggggtga gaggaacgga aggaaagatt cacgccatca 1380 cctgggccag gaagcagaag aaacccttct taggtgtgtg tctggggatg cagctggccg 1440 tctgcgagta tgcaaggagc gtcctggagt ggacagatgc caactccaca gagtttgacc 1500 cggacaccag gcatcctgtg gttatcgata tgccagagca caaccctggg cagatgggcg 1560 gcaccatgag gctggggaag agacggacca tcttcaagag cggcagcagc gtcctgcgga 1620 aactttatgg agatgcagag tatgtagagg agaggcaccg gcatcgcttt gaggtaaatc 1680 cagagctgaa gagtcacttt gaggagaaag gcttccggtt cgttggtcag gacatcgaag 1740 gggagaggat ggaggtcata gagctggacg atcacccgta tttcgtgggg gtgcagtacc 1800 acccagagtt cacgtcccgt cccatcaaac catcgcctcc ctacctgggt ctgctgctgg 1860 cggcagcagg gaagctacag tcgtatttgc tcaaaggctg ccggctttct cctcgggaca 1920 cgtacagcga tcgcagcggc agcagcacgc cagactccga gatttcagag ttgaagttgc 1980 cgtctctggg gacagattct gcgtgaagac gaattcctca ggagggagca gctgctcctg 2040 tcatgtggcc ttgtgtgatc atgtatttta agcatgtttg tagaatattt caataaataa 2100 gattcacaca ag 2112 <210> 97 <211> 1201 <212> DNA <213> Oryzias sp. <400> 97 atagtgggct tgcactttga tcaggaggtc aatcgctaca aagggaagaa ctacccgatc 60 atgaatatcc atgagagaac cctcagcgtg ctggcttgtc gatatgtatc agaagttgtg 120 attggtgcgc cattcgccgt cactaaagac ctaatagacc atttgagggt ggatcttgtt 180 tgccatggga agacagaaat atatcctgac aaggatggat ctgaccccta tgctgagccc 240 agaaggaaag ggatcctgcg catggttgat agtggaaaca gcctcacaac agacgccatt 300 gtgcagagga tcatcaaaaa caggttgctt tttgagacaa ggaaccagaa gaaagaggcc 360 aaagagatcg ctgtgatcca agcaatgaaa cagaaagaag aggaaaagac taaagagcga 420 gcccaagctg tgctctaagc acttccacta catttgttaa cactgacatg cctagtttat 480 ataactccag tgaaatcaac tattagaatt tggcttacta tagttttgtt ttgttttcgg 540 atgttttaag ttcctcaatt atctcttcca gaggaatttg ccttaaacaa aataaataac 600 ttttttatgg gctttgagta aaatgaacac gcttgaaatt tttgtttagc ccaacaggaa 660 atcaatctgt atatctaata tatctttgag atgataaact actataagac ttgaatagat 720 ctccctgatt gtctgtggac acaatgtaca gtatacccaa agcatattat taaaattgtt 780 ctaatttaat aggaatttat ttttttaacc taaaaggcac attgtggaga cctcaaagtc 840 atgcttaatg agtgcaattg ttgtttttaa tcaatatttg aaatattgac actactgaga 900 aaaggtattg ttgtcatgac ggttgtatgc cacaaaaata tgagtgtaaa atgtaactaa 960 attatatata ttttagaatt atattatgaa ttttaacaat atgatataca cataaataaa 1020 tatacacata catttttatg tagaaatata tattttgttc acctccttct aaacgtgttt 1080 agcttgtctc atgtaaaaca gaacgctctg tgttgaaatt cttgtaggct ttgatgcata 1140 tgttacagaa tactcccatt ttaataaaat gtagaaaata attggattgt gatatatgat 1200 t 1201 <210> 98 <211> 484 <212> DNA <213> Oryzias sp. <400> 98 gaggagttcc tgacacgcag ctggcggccg ccgtctttct gtctgactgg accggggcaa 60 agctaagcta ccactgtaaa gttccgggga agcacacccc tcctgcttac ctggtggatg 120 ccgtggtcac ccaaatgcag agtggctggg atttgaagaa actgaaagca ggaaatgagg 180 aaactctgac aggtgttaaa tttccaaacc ctgcgagcag catatgcttc atctctaaag 240 gtccaacttc tggtgttctg ggtgtttctg aagaccaacc cggtgctgca aacagagcgg 300 aggagaagca gaacgaggag tctgtggact cgaatgaagc cgtggagaat ccagagaaaa 360 catcacagag caaaaaaccc aaaaaagtgc agtttcagtc atcgaccttg gaggtgaatc 420 tgacggctac gagtacagac gacgcctacg actttaacgc ccattttaaa tgaacttcag 480 ctcc 484 <210> 99 <211> 468 <212> DNA <213> Oryzias sp. <400> 99 ctctgcacat aggctgaagg ctatggaccc cagttgttag ctgcggtgcc ccctagtgcc 60 tctgtcccag accagccgct tggtgagccg cctgattgaa atcctgccag aaaccacgcc 120 cagtactctc tggccttaca cctcctggag gctgtggacg tcctcatcac ggaggaggac 180 tgaagagccg ggtcctggtc tgttatacag tcatcctcat catgtctctg tcatagaagc 240 tgcttttaaa ggtttacttc atcaggttga gtgcatgaat cagaatctgg ttgaatctgt 300 gggttaagca gagcgttgtc atagaagact tggtcctctc gtgccacatg agtttagttt 360 ctctgctctc tgttagtcaa catgtaaata aagaagagcc ttatttttac ctcgcctgtc 420 cctctatgcc caactgcttt cactaagaaa taaagaggct atgagaac 468 <210> 100 <211> 360 <212> DNA <213> Oryzias sp. <400> 100 aagcagtggt atcaacgcag agtggctcag gtgacagacc tgcaggcggc tgtggtctac 60 ttggcatctg acacctccga ctacatgaca gggcataact tagtcataga gggtgggcag 120 agtctgtggt agaaggaaaa acaacttttt attctttggt ttctggatat cctctcagaa 180 atactccagg aatcgttctt ttaggaagac ggaatgatga aatgtgacca attttctctg 240 cttgtatttg atccttgaat catttattta ttttatttac ctttatttca tcaggcaaaa 300 acattaagaa caatttctta tttacaatgt tggcctgagg aaaagattaa atatcttttt 360                                                                          360 <210> 101 <211> 833 <212> DNA <213> Oryzias sp. <400> 101 aagcagtggt atcaacgcag agtgccatta cggccggggg ctcgtcccag ccgtgcaatt 60 gtcctccaac aaaatgagac ttttctcctg ccttttgttc atcactgctt ccttcatggt 120 ggaaggggct ctgtccacga ggacggtcat cttcccttat caaacaagcc acagttatgt 180 ggagatcatt cccatgaagg agatggagct gggggctttc actctgtgca tgcaggtggc 240 cacggagctc gccggcgctc ggaagatcat cctcttcgcg tacggaaaga cggacgatga 300 gctgaatgtg tggcgggagc tgaacggcgg gatctccctc tacctgggag atggttacag 360 cgactccttc aaagttcctg accccggccc cctgaactct cacctgtgtt tgacctggga 420 ctccagaaca ggcgcgacca ccgtcttcat ggacggcagg aggagtctga ccaaattcct 480 ccggaagggt cacgtcatac ctgccggggg caaagttttc ctggggcagg acccggacga 540 cgttgaacaa atgcagtcgg gcttcgatgg tgaagagtgt ttggtcggag aagtctccga 600 cgtccacctg tgggactctg tcctgtcaga cggggaaatc cgagacttgc attctgggaa 660 atcagtgctg aagggaaacg tcatcaactg ggaaaccgca aacctcagag aacgtggaaa 720 cactgagatc ataacccggc gggtgtaagt ccggggtcat cggtcctgct gcggcccaga 780 ttgaacggct ggagcaaata aaatgaaatt taaaaagcaa aaaaaaaaaa cgg 833 <210> 102 <211> 256 <212> DNA <213> Oryzias sp. <400> 102 aaaatatgaa ttccgttatg tatttgtaga gatagacacc taccccagac ggactattgt 60 tgtggaggac aacagatgag tttttcctac actaagaagt ttgcatttgt aaagcgggtt 120 tttatggctg tgaactaaat acattgcttt tcttgaatat tttgttgtga aattttaata 180 aaaatattgg ttaattgaat tattgcccag catttggtca aaaaacttct gtcacagtgt 240 caaattaaaa aaacat 256 <210> 103 <211> 1819 <212> DNA <213> Oryzias sp. <400> 103 aagcagtggt atcaacgcag agtggccatt acggccgggg cagttggatc ctgacgacca 60 tgcttgggat tttggttgcc gttgccgtgt ttttgcagac ggtctctgct gcctctctcg 120 gtgtcgtgaa caccgaggga gggagggtgg agggcaaaac catccgcctg gggttatttc 180 gtaccatgga cgtcttcaag ggcatcccct tcgctgccgc tcctggacgg tttgagaagc 240 cgaggcctca cgatggatgg gacggtgtta tgaaggccac tgagttcaag agcaggtgtc 300 ttcagctgga aattaccttg gttggtacct atggcagtga agactgtttg tacctcaaca 360 tctgggttcc ccacggctcc tcagtatcca ccaacttacc tgtcatggtc tggatctacg 420 gtggcggttt cttggctgga gctgcatcag gtgccaactt cctggacaat tacttgtatg 480 acgggcagga actggcggac agaggaaacg tcatcgtggt gacgctggga taccgtgttg 540 gaagcctggg cttcctgagc accggagact ctgaacttcc tggaaactat ggtctgtggg 600 accagcaggc tgccatcgcg tgggtgcaca ggaacattcg gtcatttgga ggagacccca 660 gcaacatcac cgtcttcgga gagtcagcgg gtggagccag cgttaacttc cagattctca 720 ctcctcacaa caaagggctc atcaggagag ccatctccca gagtggggtt gccctttgcc 780 cttgggcaat taaccacaac ccccgcaagt atgctgagga ggtcgccctc agagtcaact 840 gccccaccga ttccagaatg gctgcctgtc tgaagatgac agatcctgtg gccttgacaa 900 aggcgggaac tattcatctg tctggctctc ctgatcagcc cattgtgaac aacctggttc 960 tgtcccccgt gatcgatgga gactttctgc cagatgagcc tcacaacttg ttccacaacg 1020 ccgcagaagt cgactacatt gctggagcca atgatatgga cggacatatc ttcactggtt 1080 tggatgttcc atctatcaac aatgatttag ttgaaactcc cattgatgat gtgaagaggc 1140 tgttggccgc cttgacaaag gaaaagggac agctgggttt ggagaatgct ttctccacct 1200 actcctcaga ctgggggtca aatcccagca agcaaaaggt gaagcaaact gtcgtggcca 1260 tcgagacgga ctacattttc ctgattccaa cacaaaccgc cctttatctt catgctgaaa 1320 aggcaacaac tggtcgtacc tactcctacc ttttctctga gcctaaccgt atgggtggcc 1380 tcattgcact ctaccccctg tggatgggcg ctgaccacgc tgacgaccta cagtatgtgt 1440 tcggaaaacc ttttaccaca ccgctgggat actggcctcg tcacagagat gtctccagct 1500 acatgattgc atactggacc aactttgcca aaactggaga ccctaacagt ggagatctga 1560 aagtccctgt tgcctggcca aaattccaaa acacagacca caagtttttg gagatcaacg 1620 ccagaacgag cactgaatct gtgcaggaga tgttgaggtt gcgctacgtg catttctggg 1680 ccagtgtctt tcccaaactt ccttcactga agtaaacttg tagacccgag gaacaaatgc 1740 aaaataaagt ctgatctaag aaaaaaaaaa aaaaaaaaaa aaaggccgcc tcggccactc 1800 tgcgttgata ccactgctt 1819 <210> 104 <211> 567 <212> DNA <213> Oryzias sp. <400> 104 aaacggtgga gaggcccgaa acttgctccg tgcagtccag gatgggagac accctgcaga 60 tccactacac ggggagactt atggatggaa aggtgatcga cacgtcgctg tcgcgggatc 120 ctcttgttgt cgaactggga aagaggacag ttattcctgg tctggaacaa agcttggttg 180 gtgtgtgtga agggcaaaag atcaaagcta ctattccacc tcacctcgca tatggaaaaa 240 aaggttatcc gcccacaatt ccaggtgatg cagccctaga atttgaagtg gaggtgattt 300 ccttgtcgca gcagacgcca ctgcagaaaa tgatcaacga cgtgtttcct ctagtctgtt 360 tggctctggt gcccactttg ctcggtctgg tgggacttta cctctacaaa aaatccagtg 420 cccaaaaacc aaaacaaaaa gaagccaaag gacaagaaga gcaagaagaa ataaggagaa 480 aaagcaagag gctatttaaa ttaaagtttt taaaacaaaa aaaagaaaaa aggccgcctc 540 ggccactctg cgttgatacc actgctt 567 <210> 105 <211> 590 <212> DNA <213> Oryzias sp. <400> 105 gtctgggata cccggctcca cgctttcact tcccttactg gctggtttac ggaccggccc 60 tgctcctctg gctgctgacc gtgctcctgc ggccttttgt ctccttcaaa ccgacattca 120 cgcccatgag agtggctttg gccggaacct accactacta cagctgcgaa cgggccaaag 180 agcacctggg ctacaaaccg gtggtcaact tgaaggaggg gatcgcgcgc acagttcaga 240 gctaccccca cctcaaacac agcgcttgat tgtagaaaaa aaactgttta tacacaacat 300 cagtgccaaa aacttggcca ggttttgctg aaaggacttc ctgctcacat agctgttatg 360 tcccatttcc accagcagat ggcagcaaat agtccctttt ttgtggattg ataattgcca 420 ttccacctgt tgatacaggt atacaacaga gatgaatccg ccacaatgcc ttattacttt 480 acattcctga catatgtgta aaagcaactg tttaaggtac agttttcaga atttaatgac 540 aaaatatgta aaactaggga tcttaaatca tgattaaaac aggctgtacc 590 <210> 106 <211> 420 <212> DNA <213> Oryzias sp. <400> 106 aagcagtggt atcaacgcag agagggcatc caggccgagt ttgtgggagg agtgctggtg 60 tgcaacaaca tggtggctgt ccgcaggacg gaggccggac gcatcggctt ggaaggctgc 120 ctgtgtgacg actactacaa gatccgagag ctgctgtatc agcagtacgc tgtcgtatag 180 cagcagcagc agcaggaaat aaagctgcag aaaactgcca gacaaaatag aaaaaaaaa 240 atactttgta cattttctca ttttttatag tattttgttt ccatgagtca agatggtctc 300 tgtgtaaaca gtctggaggt ctgaacattt tgcaaaattc acccacaatt cctttgtatt 360 tgtatccgtt ttgtttcatt aaacctactt tttcttataa aaaaaagaaa aaaaaaaaaa 420                                                                          420 <210> 107 <211> 580 <212> DNA <213> Oryzias sp. <400> 107 aagcagtggt atcaacgcag agtggatctc caacatcgca gtccaccccg gaggtgatca 60 tgtgatctgt gggagttacg actgtaagct gagctggttt gacctggatc tctcgacgaa 120 accgtacaag gttctacgac accacaagag ggcggtgagg agcgtggcct atcacagagg 180 ttacccgctg tttgcctcgg cgtctgacga cggaacggtc atcgtctgtc acggaaccgt 240 ctacaatgat ttgcttcaga acccgctgat cgttcctgtc aaagtcctaa gaggtcacgt 300 gatcacgcac gacctcggag ttctggatgt cacttttcat cccacacaac cttggatttt 360 ctcctcagga gccgatgcaa cgatcagact tttcacctaa aaaaaaatgt taaattgttt 420 tggatctaca ataaagacgg gattgttttt cctttttttt agtcagataa atttcaaatc 480 tttgtctgga atattacagt cttctgtttc tcactacaga aaatagtgtc atttcctagc 540 aacattaagt tgtttgttta ataaattgta atattaactt 580 <210> 108 <211> 525 <212> DNA <213> Oryzias sp. <400> 108 aagcagtggt atcaacgcag agtgtccatt acggccgggg ggaagcccct ccgcagacgg 60 acaggtgaag aagatgacgt cctcctcgcg cctcctcctc ctgctgctcc tctccctcag 120 cgcctccttc agctgctcct ccgccgcgca gagagactcc aaactccgcc tgctgctgca 180 ccggaccccc ctgctgggct ccaagcagga cgtgtctcgc tcctccctgg ccgagctgct 240 cctctcggac ctcctgcaga tggagaacga ggctctggag gaggacgggt tccccctgag 300 cgaaggcgag cccgaagaca tccgggtcga cctggagcgc gccgccgcca gcggcccgct 360 tctcgccccc cgggacagga aggcgggctg caagaacttc ttctggaaga ccttcacgtc 420 ctgctgaggg ccggcctcgg cagaagctcc tccccctgcg gacgctctca ttagtttgtt 480 tagtattttt cttggtttga tctgaacttg atgaaaacta ttttt 525 <210> 109 <211> 270 <212> DNA <213> Oryzias sp. <400> 109 aagcagtggt atcaacgcag agtgagactt ggacatggcc tactctagtc tcaatgctga 60 tgggacttgc tggagaaaga acagccttgc gaagaaaaca agcgtacctg ggaagttcac 120 gtatacgtgt caaagatggg gaagtttgaa tgacttgagt gtggttgatg caaaatatga 180 tgaagtatgc cctgatccan accctcaaaa caaaaggaga tgatgtctac agcgtcacct 240 aagctctatg gccgcagtcc tgacctcagt 270 <210> 110 <211> 1160 <212> DNA <213> Oryzias sp. <400> 110 aagcagtggt atcaacgcag agtggccatt acggccgggg gtaggtcagg agggacgcca 60 tgtgctgttg aggcaggggt gcaggagaag gaccgggctg gaccagcaga acagattcgg 120 ttgaaaattc agcgggacag cggggagacg tattccgtga acgttagcat gtactgcacc 180 agccgctgcc tccggagcgc gctgcgcgcc gccgccagca cgcaccggta ccgacaacag 240 ctccaacatc aggttccgtc cttgtgtgcc ctcagacttc tgaagaccag ctcatcctct 300 cattcagaaa ctattgccac gcctccatta gacggagcac ccaaacagta ctcccccaaa 360 attcagcagc tcgtcaacga cattgccagc ctcaccttac tagaggtgtc ggatctgaat 420 gagcttctga agaaaactct gaacattcag gatgttggaa tgatgccgat gggggcaatg 480 gcagcaggtg ccatgccagc aacgccggct gcagaggagg aggaggcacc ggccaaaaaa 540 gagaagactc acttcacagt caaattgaca gaactgaaag cagcagataa agtaaagtta 600 ataaaagaag tgaaaaattg catccaaggc ttgaacctgg tccaagctaa gaagcttgta 660 gaatctcttc cacaagaaat ccgggccaac gtatccaaag aagaagctga gaagctgaaa 720 gcagccctgg aggctgcagg cggcactgtg gtcttggagt aaatcagtgc atttctacat 780 ctgcctctta cattgccatt tctctggcca cgcacttcgt ttttcattat tttataactt 840 tttttttttc ctgggcgaag acgtggacac tgcaaaagaa gcaaatgcct tcatttactc 900 gaagcgtcca tagcatcagg tggggtgctg cagattgaac ttttccaggg acacggcctg 960 cacaaaccac ctccttgcag tttgtttgtg tgctcccgtt tgtctgaaac tcgtgtagca 1020 aacgctacca aaactctggc ccccggtgcc aggcggtatt tctgtgtgtt tgtcaagccc 1080 ttcataaagg gacggtttcg ctgtgaaagt aggaatactg gaacagcagg aactgtattt 1140 gactctgaaa tgagtagtta 1160 <210> 111 <211> 747 <212> DNA <213> Oryzias sp. <400> 111 tccgaactgg aggccctgga gtgtcacttc acctgggtca aggatgtcac tagatcacag 60 ttgttggaca tgaaggtcaa gcttgaggac attgggacac atgagggaaa tttttggttg 120 ggctttattt acaacatgtg ggggtttgtc ctctacaagc tggactctag tgaagaagcc 180 cttcagttcc tcaggaaggc cacagaaacc ttaaagcgct tgagggagga agatgagggt 240 ccctggttgg tggtgaacta tgggaatctg gcctggctgc accatcatct tggagaagag 300 gcaatgagtc aggactacct gtcaaagatt gagctctcaa aagtaaattc ccatctccat 360 cccaggacga gctccatcca gagatctacg ccgagaaagc ctggatctga tgaagttagg 420 taaagagaag aagcagctgg ctgcagatta ctttcagata gccttcaaca tgcagccaga 480 catggtgcag tggcaatcca gccgtgtcat tgcactggcc agtgctgcta agcactggga 540 cacagacctg agtgatgact tcttgcagga aataagaaca gcccgagagc aggatccaga 600 gaatctgtac gtggctgctg tggagcttaa agtaagaaac agaaaaggag aatcagtgaa 660 agatgaggcg gagacgctgg gccagaagat cctcctgcag cctgtcagca gctacagtgg 720 tctgaagcct ctcctgaggc tgcacag 747 <210> 112 <211> 2103 <212> DNA <213> Oryzias sp. <400> 112 aagcagtggt atcaacgcag agtggccatt acggccgggg atgttctgtg agaaaggaca 60 accattgaca gctgagaaca ggagaaatgt atcaagcatg tggaggagtt gtattcttta 120 gtctatgcct cattttcttg gtgccatcat gcagtggagg aaacattctg gtgtaccctg 180 ttgatggaag ccattggata aacatgaaga ttcttctaga agaacttcat gccaggggtc 240 acaacatcac tgtgatcagg gcttccagca gctggtacat taaagagaag tctcctctct 300 actcatccat caatcttgaa atggaagagg cctttgaaga tttctttgat attttcgtag 360 ctgagcaaat taaggtgcag cgagaaggtg cttcaatgtt gacgttcctc aaactcaaca 420 gagactttct ttcaatgctc acccaggctc atgtattgtg gtgtgatgtt acgaaagaca 480 tctttgaaaa taagactctg gcaaaacaaa taattgattc aaagtatgat ttggttctga 540 ctgatccagc aataggtgga ggtcttgtct tagccaaata ttttaattta cctttggtac 600 tcaatgtacg ttggatccca cttggagaag gccatcttgt tctggctcct tctccacttt 660 cttatatccc attaccagga tcaggcttaa ccgatcaaat gaactttatt cagaggctga 720 aaaacatatt tttttacggc atctcaaagt ttcaggaaat tgtcttggtg ggacccattt 780 atgatgacat ctgcagtaaa tacatagagg ggggatgtag tgtcgcctct ctgcttcagc 840 aagcagacat ttggcttttc agatcagatt ttgtgtttga cttccctcga cccaccatgc 900 caaacgtggt ctacatcgga ggattccagt gcaaaccagc tgaacctctg ccagcggatc 960 tggagaagtt tgttcaaagt tctggagaac atggagtgat aatcatgact ttgggaacac 1020 tagtcaatgc tctgccaaaa gacattgcag atgagattgc catggtcttt tccaagatgc 1080 cccagaaggt gatatggaaa cacaaagggg accgtccctc tacactgggc agtaacactc 1140 tcctggtgga ctggatgcca cagaaagacc tccttggtca tccacaggtt aaagtctttg 1200 tagctcatgg aggaaccaac ggagtccaag aggccattta ccacggagtg ccagtgctgg 1260 gcatcccact gttctttgat cagtacgaca acctattacg tttacaggaa agaggagctg 1320 gaaaaatcat tcaacttgcc gacttgaata gccacacctt tgaagaaggt cttcaggaac 1380 tgctccataa tactagctac aggcagaaca ttcaaagact ttctcgtttg cacaaagatc 1440 aaccaatgtc acccatggat caggctattt tctggataga gtatgtgata cggcacaaag 1500 gggctcgcca tctgatttca gaggcttaca agatgccttg gtactcatat cacagtttag 1560 atgttctcct tctgctgctg gctgtactaa cggtgctgct ttactccatt tatgctgttt 1620 tcagatgtct atgctgcaga aggaaaagaa agacaaaaac taaacaaaac tgaagttgtt 1680 agatttgttt gaaaaaaaat gtgtttggaa tttaaatttt atgcatgtat ttaataaaac 1740 ataaaagatt ttttttttta ttatgttgca attttgaaac caagggattt ggatgattgg 1800 ctcataaatt tacatggtaa cttattacac aaatctagtc agaggttctc tctgaactat 1860 ttttttaaaa ctttttcttt cattgcaatg tatgtacttg ttcttgtttt aattttccaa 1920 tcacaatccc atcgttaact tgttgaatgt aacactttct tattgcaatt accacttaaa 1980 ttttaccagc tctcaaatac ttttgctttc tcaacatttt gagttctgct tctgatctca 2040 attttctttg caaattttat ctttttaaat tagtatatat cattaaagta cttttttaaa 2100 tCt 2103 <210> 113 <211> 993 <212> DNA <213> Oryzias sp. <400> 113 aagcagtggt atcaacgcag agtggccatt acggccgggg acctgcaagc gctgcacgag 60 gctgccagac acagaggtcc ttcactgtaa cgtaaagaaa aatcagaaat gggcgtttct 120 tcggcacaag ggagtcttca tccgtactgg ccgcgggacc ttctgattcc caactatgtg 180 gctaatgacc gctccatgtc cgagattctg gctttccttt tctcggtgtc gggggtgttt 240 ctgctcgcca cctggctgat taccggggcc gcggacaggt ttgggatctg gaggcggctg 300 gctatttgtt ggtttgcagt gtgcggtttc atccatggtg tcattgaagg ctggttctca 360 ctttattacg acattctacc tgcagaccag agtttcctgt cacagctgtg gaaagagtat 420 tcaaaaggag acagcagata tgtaatagct gataacttta ctgtctgcat ggagactgtg 480 actgcttgtt tatggggtcc cttcagcttt tgggctgtat actcttttct atcacataaa 540 ccctacagat ttattctgca gctcatcatt tcattagggc agctgtatgg agccgtgctt 600 tacttcttca cagaacacag agacggctat gcccacagcg agttgggaca tcccatctac 660 ttctggttct actttgtgtt catgaatgtt ctatggattg tcataccact aatgcttatc 720 gtggatgcat ggagacagct atcagctgcc cagaaacaca cagatggcat caggacccac 780 aagagtaaaa agaagtaatc tcaggatacc aagggactga aagacactct ccttcttaac 840 aatctttatt tcaacaacca gacattcctg atgctgatta aagcagaaag actacaatga 900 ttgaatgtca aattattgta ggcaaaactg aaattttctt gttttgtact tttttaagaa 960 atctgcttct gacaattaaa atatatatat ata 993 <210> 114 <211> 2171 <212> DNA <213> Oryzias sp. <400> 114 aagcagtggt atcaacgcag agtggccatt acggccgggg ctgactctgc tgtgagggca 60 tcgggccaac tccgagaggt cctcccactc tcctccaacc aggcaggcac ccggctgcgc 120 acggagctcc tcaccgccac agtctcaccg cctcctccgg acgcagcaga cagcgcgcgg 180 ctcctccacc tcctcagcgc tccatcagct ggctcctcaa atggtcaaaa atggactctt 240 taaatcataa cctcaacact tactttgaat catggctcgg ccctagagat aagcgggtgc 300 agcaaatgct tttgctcgac aactacctgc caacctttgc acttaccgtc atgtaccttc 360 tgatcgtgtg gttggggccc aagtacatga gacaccggca gccgtattcc tgcagaggtc 420 tcatgttgct gtacaatctg ggcgttactg tcttgtcctt ctacatgtgc tatcagcttg 480 ttagcacttt ttggagcggt ggctacaact tttactgcca gaacacatac agcaaaccag 540 aagcagattt aaagatcgta aatgccctgt ggtggtacta cttttccaaa ctcattgagt 600 ttgcagacac tttcatcttc atcctacgaa agaacaatca tcagatcaca tttcttcacg 660 tctaccacca cgctagcatg ctgaatattt ggtggtttgt catgaactgg attccctgtg 720 gtcactcata ctttggtgcc tccctcaaca gcttcgtgca cgttgtgatg tattcttact 780 atgatctctc atccatccct gctctccggc cttatctgtg gtggaagaaa tacatcacgc 840 agctccagct gatccagttc gttttaacca tatgccagac tgcctgcgct gctatatggc 900 catgcggctt ccccatcagg tggctgtatt tccagatcag ctacatgggg acgttcgtcc 960 tacttttctc aaatttctac attcagacat acaagaagca gagtgcctca cggcagaagg 1020 agttcaagaa tggttcttct atgtcgacga acggtcacgc aaacgggacg ccgctggtgg 1080 agcgcgccgc atccaagaag ctgaggacgg attgacgttg gagaaagcct cactcaagat 1140 tagctgtagc gtgttagccg atgctgctag ggggcgtagg cgtcctctct tctagaatag 1200 tcaagaacta atttacaata aaacaagcca tagccacaca tacaatacaa aggagacaaa 1260 aacacaaata ccacttattt ggtttaatca tgttaccctt ataattatag aagaatacta 1320 atgcactagt gttgacacag caataaactt tctttgcctt ttatgctaat ttatttcaga 1380 acagaatgtt ttttttcact gccagagcaa aaaaaanaaa aaaaaaacta cacatctaca 1440 ctcattcagt gatgctttac acaactaaag tccaaagatg aacaatcaga tgtgttgaca 1500 gataaatgtt ttatgacaga tcagtgtgag aatgaaggct atgatctttg aacgtttatg 1560 ccactccggc cagcctacct gtgtgtgtgt ctacatattc cagaatgatt gttctcagta 1620 atactttcaa tcatcaatct ctacaaataa agagcttcaa cagctttttg caggtgtgtg 1680 aaaggacaac tgttgtattt gtgtaaacat gtcaaagaaa ctcagttttg tcagtattac 1740 agaaaatctg tgtctcactt tgtccttttg gcaccttatt taaaatggtc atgtctgttt 1800 tgttgtggag aatcacagag tttgaaaata caactatgca gtacagttta ttgttaaata 1860 ttacagtaga attacagttg gatttgctgc accatttagg acgtaatttc tttcaatttc 1920 tgcataaaga tgacttaatg tatgtttaat aaaggtaatg gtataagttt tatgtatgtg 1980 tctggcaagt ttatttcaag ttaaggtcac aaacatcaat caacagtttt acaactctca 2040 atttctgctg aaattcagtt tgttgtgaca atttaacatg gtaatttaca ttttttgagg 2100 gaagttgtgt tttatttgct atttacattt tttgaaacta caatttgcat taaaatttat 2160 ttaatataac t 2171 <210> 115 <211> 545 <212> DNA <213> Oryzias sp. <400> 115 caaggtcttt gctgttgcca tctcgccaga ccaggaggac acccgactct cactcattgc 60 cacaaaccag aactatcgac agaacttcac agctgcagac aacatcagat ccaccaagat 120 gcaaaccatt cggtccatca ttgacatcat taccaacgaa acaaaggatg tgtgctgctc 180 ctttgactgc aacccttccg gaggagccaa aggaccacct ggagatgccg gtggaatggg 240 agagatgggt agacctggga tgccaggaga gaaaggagac ataggcaatg tgggcagcgt 300 tggagatcct ggacctgttg gctacagtgg gatgaaggga gatcgaggtc caagaggagg 360 aaagggtgaa agaggagcca aaggctttaa ggggacaaag gtcaaagggg gttagatggg 420 actgatggac ggaagggtga ggctggtttc cctggacttc ccggatgtaa aggttctcca 480 ggaggcgatg gccagcaagg ggagcctgga ccaaagggag atgctggtcc ttatggcagg 540 aaagg 545 <210> 116 <211> 405 <212> DNA <213> Oryzias sp. <400> 116 aagcagtggt atcaacgcag agtgtcctct tcttctttag cgtcatcatc atcttcatcc 60 tcctcctcct cctcagagga agcctcaaac caaagccctc tcaacctgag cccagtggac 120 tgcctgctgt gtccggtgtg cggggagagt ttcaccagca gggccggcca ggagaggcac 180 ctgcgcctca tgcactcctc ccagatctac ccgtgcaagt actgccccgc caccctctac 240 agctcaccgg gcctcaccag gcacataaac aagtgccacc cctcggagaa caggcaggtg 300 atcctgctgc agatgccggt gcgccccgcc tgctgaagac aacacagaac tcgtgccttt 360 gatggatgga gggaggaagg aaggagaaga aaaaaagaag aatgg 405 <210> 117 <211> 961 <212> DNA <213> Oryzias sp. <400> 117 aagcagtggt atcaacgcag agtggccatt acggccgggg gttttgagca acgttagaaa 60 aagagctcca ggtgagaatt tggttgcagc tgaagaagat gtacgagcct ctggaagaca 120 gtgttgagat tacgttccct gtgaagtttc tgggccgggt ggaggtggtt cgttctgatg 180 gacttcagat cctggaagag gcggcttaca gtctaaagac cccagataag tattcatctg 240 agaaggtgac gaagaacact agagtcctca tcttcctatc tctgagtggg attgacatct 300 tggagcataa aaccaagttt ctgctgtacc agtgttctct ctccactgtc tccttctgcg 360 ccgtcctgcc atcttcaccc aaagttttcg gttttgtggc caaacatcct gcctcagata 420 tctaccactg ctacttgttt cagagcaaag cctatgccca cgtcctggtg tccgccatcg 480 gagatgtctt cagagcgtcg aaaaatcagg aaagcgtcag aggagggcga gatctgatcg 540 tggaggccct caggcacaag aataaaatac ttcagaagga gaacgatgag ctgaagagga 600 agcttgcagc cctgcagaaa taaaaagatg gctcatacgc aatcaatttt tcaacttcct 660 gtttcaagtt cgttccagaa aacgcttcaa aaacctgcaa gaaacatgaa aaaaaaggtt 720 tttcctaaag ctccaaatct ttacatttaa tttaaataat ttaatgattt tttttcttat 780 ccagcacttt actgttcatg atactgaaac cttttttccc aaagtgtaaa gataaatgtg 840 ttgcttttga ctacatcctg tttgctctgt acattcattg ttcggtgctt tagaaaaata 900 aatccttttt gccaacctgg gatataaata caacaaaaat gttcaaacag taaaataaaa 960 t 961 <210> 118 <211> 538 <212> DNA <213> Oryzias sp. <400> 118 aagcagtggt atcaacgcag agtgccatta cggccgggga acacaaactt ttgtttaaag 60 ctcacgatgg agcctgccat cgatgtcctg atgtctaagt ttaaggtcta cgctggaatt 120 gatggttcat ccgtgactct gagcagggat gagtttggaa aactggtgaa atcagagctg 180 cccaactttc tgcagaattc tggagaccct gccgccatcg atgagctcat gagatcattg 240 gacaagaaca acgatggaga gctgagcttc atggagttct ggcagctgat tggacacctt 300 gccagcaagc atgggggctt agaccaatag cgtctcaaca gacagcttcc atgcgttcca 360 gttctgtcag cgtgccatcc tcgcttctgc ttttctttca cgccctgtct gaccaaagtg 420 aacccggtgc tgccacagga gctggtcgtg cgcagctgcg ctctgattta cgcgcacatc 480 tagaatcatg gaacggagtg ggaatgcatt gtcttcatga gcagtttctg gctcctct 538 <210> 119 <211> 431 <212> DNA <213> Oryzias sp. <400> 119 aagcagtggt atcaacgcag agtgccatta cggccggggg tctgagtctc cagctcgagg 60 agcttcagtg tggcagcttg acccaacctg gaccgatacg ttctgcaagc atggtggata 120 ctttctgcgc tacgtggaag ctggtggaca gccagaactt tgatgaatac atgaaggcac 180 ttggtgtcgg ttttgccaca agacaagtgg gcaacgtcac caaacccaca atagtgatca 240 gccaggatgg agataaggtg gtggtgaaga cccagagtac cttcagaaac acggagctct 300 ctgccaaact gggagaggag tttgacgaga cgacacctga tgaccgtcac gttaaatcca 360 ctttcaccat ggacggagac aaatttgtgc acacgcagaa gtgggatggc aaggagacca 420 agtttgtgag a 431 <210> 120 <211> 649 <212> DNA <213> Oryzias sp. <400> 120 aagcagtggt atcaacgcag agtggtggtg tccgacggga tcggcttacc gaacgcgctg 60 gcctacgact actccactga tcaggtctgc tgggccgatg caggtacgaa gcgtttagag 120 tgcgtatctc caaacggctc cggcagcaga gtgatccatc ccaacctgaa ctacccgttc 180 agcatggtct actacagaga ccacttctac tacacagact ggaggaggga cggcgtgatc 240 gcagtgaaca gagacagcag caagattacg gaggagtacc tacctgacca gcgctcacac 300 ctgtacggca tcgctgtagc aaccaaccac tgtctaccag ggagccacta accaccagcc 360 ggcagctatg gggggggcct atcggtgcca cagaggcagg agacggtcca ggaggagccg 420 ctcccctccc cccgctctga agatgtaacc caaaagttct gtacatttgt tttatacctc 480 atttctaaag tttttactgt atttttttta tattttgtat cgattaaaag ccacaatcac 540 ctaaagattg ttcaactctg aggccaaacg tcaccagaaa cggagaagaa ataaacagag 600 aaactgcctg aactcacctc tgcactaact tctcaccttt tttttgttg 649 <210> 121 <211> 2145 <212> DNA <213> Oryzias sp. <400> 121 aagcagtggt atcaacgcag agtggccatt acggccgggg agctggagtc caatcaggca 60 cgtggggcaa aattcttcaa catctcagct aagaagtggc actgagcatg gaacaacaca 120 tgaatggcca gagccctgag acgggcttaa ggaatctcag caaattctgt caggctgtca 180 gagatggtct tactctggag gaatcagctg tggaaatgaa agcagctgag tgcttcatcc 240 acgaagccat ggaagtgatt attgatgaag cagtaaaaaa ggccactgat gttcaagaaa 300 aggtatgtga gtggcactcc cctgaacagc taaaagagct gctggacttg gaactgaaag 360 atggagggga atctgactct aaaattctgc agcgctgcaa aaatgccatc agatacagcg 420 tcaagaccag tcatcctcgt ttttttaacc aactgtttgc agggctggag ccttactcta 480 tggtggcaag cttcatcatc gaagcagtca aaaccagtct gtacacatat gaggtggccc 540 cagtctttac cttgatggag gatgctgtgt tgaaaaagat gatggagttg atcggctggg 600 atgagggagg agatggcatc tttaatccag gtggatcaat atccaatatg tatgctgtga 660 gtttagccag gtatcgattc tgtccagaca ttaaagaggt tgggttctat gatgctccac 720 gactggtcat gtttacatca caggagggac attattcaat ttccaaagct gctgctttgc 780 tgggatttgg cacaaaaaat gtttatgtgg ttccatccga taacagagga aagatgattc 840 ctgcggctct ggaagagaaa atagagttgg cacgtagtga aggcgcagtc cccctcttgg 900 tgaatgccac cgcagggacc acagtgttcg gagcctttga tcctgttgac aagatcgcag 960 acatctgtga gcggcacagt ctgtggctgc atgtggatgc atgttggggg ggtgcagctc 1020 tgctttctaa tgagcataaa cacctgctaa aaggcattac cagagctgat tctgtagctt 1080 ggaacccaca caaaatgctg atggcttgtc tgcagtgttc agcattcctg gtcaaagata 1140 aaatgggtct cctccaacgc tgccactctg caaaggcatc atacctcttt cagcaagaca 1200 agttctacga tgtgagctat gacacagggg acaagtcagt ccagtgcagc aggaaacctg 1260 atgcttttaa gttctggctc atgtggaagg ctttgggatc tacagagctt gagcgtaggg 1320 ttgacaaagc tctggccatg gccagatatc ttgctcaaga agtcaagaaa agagagggat 1380 ttcgtttgtt aatggaacct gaatatgcaa atgtttgctt ctggtacttt ccaccggcgt 1440 tgagagacat tcctgatggc cctgaactct ggagaaagct acatactgta gcccctgtgg 1500 tcaaggagcg gatgatgaag acaggcagca tgatgattgg ttaccagacc catggggaca 1560 agccgaactt cttcaggatg gtggtcatca atccccaagt cagcaaaaaa gacctggact 1620 tcgtcttgaa tgagatccat aacctgggaa aggacttaag atgaaacatg aacacttttc 1680 agctctgctg acagcagcag aagattacagta ttaatttctc tacaatacaa ttcatagtga 1740 ttattgtgtg taaaagtttg tttgctaagg caatactcat ttggaagtct gtcttttgta 1800 tcctatactg cccttgattt ctgacctggt ttgttttgag cattgtttgg gggtgctttt 1860 ctttgtgcct agtgctaaca ctttcaatct ttggacacca gttttgggga tttttgtctt 1920 ttgctgcccc ttcctcactg tttttgtttg catgtctatg gactccggtc gcattttctt 1980 gtctactgcc ttattttaat gggtggtgtt actcaccctt cccctgcctc agtttttttg 2040 tgggtggggg tattgtgtcc tgttgctaca gttagaagac aataaaaaca tgttcatcct 2100 agtttaataa aaaaaaaaaa aggaccgcct cggccactct gcgtt 2145 <210> 122 <211> 1333 <212> DNA <213> Oryzias sp. <400> 122 aagcagtggt atcaacgcag agtggccatt acggccgggg gcttggcagc tggtgagagc 60 aaggacggtg gtgtcgacgc tagaagtttc tttgcagaca ataaccatcc aattcggaat 120 ctaaaacctc ggaataagga aactggacac gtttgaattc gcttaaatta ctcttcagca 180 gctgttgaat actgcaacca tgccagaagt ctttgcaccc gcagacatgg gcagctcacc 240 tccatccccc gtggacagca gtccgaggag actgtcctgg ggcaaactgg tccacaggtt 300 gacagatttc agcacaaaca gcagcagtat cacgtcaggg tccaacaatg gcagcaggag 360 tgacctctcg gaatctgggt ccgatacctc cagtgacctg tcaccgtaca atgatgacct 420 cttcttcgat ccgatggagg aaaccatcct gaaagaagtg gtggacctca tcgcatccag 480 ccttagagaa gccaaagact cggactgtgc tctgaggtgc gccaagctcc tcattcccga 540 gaaactgctg gagcacatcg gacgggagct ccttcatctg gctgccagcg agccctgcgg 600 attgaggggg gctctcattg acctttgtgt ggagcaagga gatgtgtgtg agagcatggc 660 tcagctatcg gtggacccct gcctcgtccc cacttttcag ctgactttgg tgttgaggct 720 tgagtccggt ggcttgtggc caaaaattca aggacttttt agctccagat ctacttccac 780 tccagcagtc cgacaagcac ttaaactcag cactggtttc cgcgtgatca aaaagaaact 840 gtactgttct gaagacctgc tcattgagga atgttaagaa atgaaagact cgtttttttt 900 ttaatataat gcgagcttac aatgaaagct caaagaaggg ccttttaaga ttatgcctac 960 agaagtataa agaaggactt tgttctgatg tagcctttgg ttcctgctgt aagtttctgg 1020 tacttggatg tcttcatgca gcacttgctc aaaacttaag tttaaaaaat gtaaccatat 1080 aatatctggt acatctgcat ttgaaactga atttgaattt taaatgcacc gacgtttcta 1140 ctgaaacatt gaaaaaggca gctagctgta ttgtgttacg cattgaagac aactgaattg 1200 tacggcaact catgacatgt actccaattc ctcagtttgg gaacatttaa gctaaagctt 1260 gcacatgtgt aagcctccac tttgatatca cgtctaagtt tttttttttt ttttgttttt 1320 ttttttcttt tac 1333 <210> 123 <211> 2120 <212> DNA <213> Oryzias sp. <400> 123 aagcagtggt atcaacgcag agtggccatt acggccgggg agtcagagga tggtttatat 60 gatgcttgcc aggctttttg agcgaatctg cattgaaaag actctgctga tgtgaaagga 120 gccaagaatc tggatttggt gtgaaacttt cagccagacc gttccgttac caagtttaag 180 tcagcagcag cagcagccga gaggatggga ggtggaggcc agctgtctgg gagcgtggag 240 cccggcagtg ggagagattc aggtgttttt acctgggagg aggtgcagaa gcactgcacc 300 aggaccgatc agtggctggt gatcaatcga aaggtttata acatcactca gtgggtcaaa 360 agacacccag gagggtctcg tgtcattagc cactatgctg gagaagatgc cacggaggca 420 ttcactgctt ttcaccctga tctgaggttt gttcagaagt ttctgaagcc tctgctgatt 480 ggggagttgg ccccaacaga gcccagtcaa gacagaaaca agaatgcctc catcatacag 540 gattttgaga acttaagagt tcaggcagag aagaatgata tgtttaaaac taagcctctg 600 ttcttctgct tccatctggg tcacatctta ctgctggagg ctcttgcgtg gctcctcgtc 660 tggttatgtg ggactggttg gacgatgaca tttttgtgct caataatttt ggcaactgct 720 cagtcacagg ccggatggtt gcagcacgac tttggccacc tctctgtttt tagaaagtcc 780 aaatggaatc atctggtgca caagtttgtc atcgggcatt taaagggagc ttctgccaac 840 tggtggaatc accgacattt tcagcatcac gcaaagccca acatcttcag gaaggaccct 900 gatgtcaaca tgctggacat ctttgtactc ggaaacactc aaccagtgga gtacggagtt 960 aaaaagatta aacggatgcc ctacaaccac caacaccagt attttttctt ttgggtccac 1020 ctctgctcat tccagtttac ttccacatgc agataatgaa gactatgatt caccatcatc 1080 actgggtgga tctggtttgg tctttatcct actaccttcg ctttttcttt agctacatcc 1140 ccctgtatgg tgtgtttggc tcttttgccc tcctcacctt tgtcaggttt ttggagagtc 1200 actggtttgt gtgggtgact cagatgaacc atctaccaat ggagattgag aatgagaagc 1260 accaggactg ggtgacgatg cagttgcagt ccacatgtaa cattgagcag tccctcttca 1320 atgactggtt cagtggacat ctcaattttc aaattgagca ccatctgttt ccaacgatgc 1380 cccgccacaa ctaccacctt attgccccac aagttcgtgt tctttgtaag aaatatggga 1440 tcccttacca ggtcaaatct ttatggcaag ggattgtgga tgttgtcagg tctctgagga 1500 cttctggaga gctgtggctt gatgcatacc tgcacaaata aaccaaacaa caataaaata 1560 aaaaatgtgt ttgcaataaa ataaatattg tacctttgtg gatccactta cacagtttgg 1620 attcataatt tgttttcagt gaagtttaaa ttataaagtt tttagtcttg ttatcggact 1680 ggaaaggggt ttttgattag attcttctga acaaatgttg gtaatataaa taaaacttta 1740 aaatctgtaa gtcacttgaa attatttaca tctggtgatt ttatatgagc aatgaatctg 1800 aaataagtta aattattttg acacgaaccg taacattaat atgaaactga atcatgtaaa 1860 ttctttataa cttgtgaaaa taagcaactt ttacactttt acacaacaca ataaaaacct 1920 gaaatgaaat tgttcattat tttgtaattt cttaagttta aatgatcact gttctgttct 1980 ttttaaggat ctcctacatg tatagtacat gcattcttgt cagatttttg ctgtaattaa 2040 aaactatttt gagacctgca taaaaaaaaa aaaaaaaaaa aaaaggccgc ctcggccact 2100 ctgcgttgat accactgctt 2120

Claims (12)

서열번호 1 내지 123으로 기재되는 모든 유전자 각각의 핵산 서열의 올리고뉴클레오티드 전부 또는 그의 상보가닥 분자가 집적된, 시료내 노닐페놀(Nonylphenol, NP) 노출 여부 검출용 마이크로어래이 칩(microarray chip).
A microarray chip for detecting the presence or absence of nonylphenol (NP) in a sample, in which oligonucleotides of all the nucleotide sequences of all the genes of SEQ ID NOS: 1 to 123, or complementary strand molecules thereof are integrated.
제 1항에 있어서, 상기 유전자는 바다송사리(Oryzias javanicus)로부터 유래된 것을 특징으로 하는 노닐페놀 노출 여부 검출용 마이크로어래이 칩.
The microarray chip according to claim 1, wherein the gene is derived from Oryzias javanicus .
삭제delete 1) 피검 시료에 노출된 실험군의 바다송사리와, 정상 대조군의 바다송사리에서 각각 RNA를 분리하는 단계;
2) 단계 1)의 실험군 및 대조군의 RNA로부터 cDNA를 합성하면서 실험군과 대조군을 각기 다른 형광물질로 표지하는 단계;
3) 단계 2)의 각기 다른 형광물질로 표지된 cDNA를 제 1항의 마이크로어래이 칩과 혼성화시키는 단계;
4) 반응한 마이크로어래이 칩을 분석하는 단계; 및
5) 분석한 데이터에서 제 1항의 마이크로어래이 칩에 집적된 유전자 발현 정도를 대조군과 비교하여 확인하는 단계를 포함하는, 시료내 노닐페놀 노출 여부 검출 방법.
1) separating the RNA from the sea cucumber of the test group exposed to the test sample and the sea cucumber of the normal control group, respectively;
2) labeling the experimental group and the control group with different fluorescent substances while synthesizing cDNA from the RNA of the experimental group and the control group of step 1);
3) hybridizing the cDNA labeled with different fluorescent substances of step 2) with the microarray chip of claim 1;
4) analyzing the reacted microarray chip; And
5) A method for detecting nonylphenol exposure in a sample, comprising the step of comparing the degree of gene expression integrated in the microarray chip of claim 1 with the control group.
제 4항에 있어서, 상기 시료는 생체시료, 식품시료, 화학시료, 공업시료, 임상시료 및 환경시료로 구성된 군으로부터 선택되는 어느 하나인 것을 특징으로 하는 시료내 노닐페놀 노출 여부 검출 방법.
5. The method according to claim 4, wherein the sample is any one selected from the group consisting of a biological sample, a food sample, a chemical sample, an industrial sample, a clinical sample, and an environmental sample.
제 4항에 있어서, 상기 단계 2)의 형광물질은 Cy3, Cy5, FITC(poly L-lysine-fluorescein isothiocyanate), RITC(rhodamine-B-isothiocyanate) 및 로다민(rhodamine)으로 이루어진 군으로부터 선택되는 어느 하나인 것을 특징으로 하는 시료내 노닐페놀 노출 여부 검출 방법.
5. The method according to claim 4, wherein the fluorescent material of step 2) is selected from the group consisting of Cy3, Cy5, poly-L-lysine-fluorescein isothiocyanate (RITC), rhodamine-B-isothiocyanate (RITC), and rhodamine Wherein the nonylphenol is present in the sample.
1) 피검시료에 노출된 실험군의 바다송사리(Oryzias javanicus)와, 정상 대조군의 바다송사리에서 각각 RNA를 분리하는 단계;
2) 단계 1)의 RNA를, 서열번호 1 내지 123으로 기재되는 각각의 유전자에 상보적이고 유전자를 증폭할 수 있는 프라이머 쌍을 모두 사용하여 정량 실시간 RT-PCR(Quantitative real-time reverse transcript polymerase chain reaction, qRT-PCR)을 수행하는 단계; 및
3) 단계 2)의 유전자 산물을 대조군과 비교하여 발현 정도를 확인하는 단계를 포함하는, 시료내 노닐페놀 노출 여부 검출 방법.
1) separating the RNA from the Oryzias javanicus of the test group exposed to the test sample and the sea lion of the normal control group, respectively;
2) The RNA of step 1) was subjected to quantitative real-time reverse transcriptase polymerase chain reaction (RT-PCR) using both primer pairs complementary to each gene described in SEQ ID NOS: , qRT-PCR); And
3) comparing the gene product of step 2) with a control group to confirm the degree of expression, and detecting the presence or absence of nonylphenol in the sample.
제 7항에 있어서, 상기 시료는 생체시료, 식품시료, 화학시료, 공업시료, 임상시료 및 환경시료로 구성된 군으로부터 선택되는 어느 하나인 것을 특징으로 하는 노닐페놀 노출 여부 검출 방법.
8. The method according to claim 7, wherein the sample is any one selected from the group consisting of a biological sample, a food sample, a chemical sample, an industrial sample, a clinical sample, and an environmental sample.
제 1항의 마이크로어래이 칩을 포함하는 노닐페놀 노출 여부 검출용 키트.
A kit for detecting exposure to nonylphenol comprising the microarray chip of claim 1.
제 9항에 있어서, 스트렙타비딘-알칼리 탈인화효소 접합물질(strepavidin -like phosphatease conjugate), 화학형광물질(chemiflurorensce) 및 화학발광물질(chemiluminescent)로 이루어진 형광물질군으로부터 선택되는 어느 하나를 추가적으로 포함하는 것을 특징으로 하는 노닐페놀 노출 여부 검출용 키트.
The method according to claim 9, further comprising the step of adding any one selected from the group consisting of a fluorescent substance consisting of a strepavidin-like phosphatease conjugate, a chemiluminescent substance, and a chemiluminescent substance Wherein the nonylphenol is present in an amount of from about 1% to about 10% by weight.
제 9항에 있어서, 혼성화에 사용되는 완충용액, RNA로부터 cDNA(complementary DNA)를 합성하기 위한 역전사효소, dNTPs(deoxynucleotide triphosphates) 및 rNTPs(ribonucleotide triPhosphates, 사전 혼합형 또는 분리 공급형), 표식시약, 및 세척 완충용액으로 이루어진 반응 시약군으로부터 선택되는 어느 하나를 추가적으로 포함하는 것을 특징으로 하는 노닐페놀 노출 여부 검출용 키트.
10. The method according to claim 9, wherein the buffer solution used for hybridization, reverse transcriptase for synthesis of cDNA (complementary DNA) from RNA, deoxynucleotide triphosphates (dNTPs) and rNTPs (ribonucleotide triphosphates, And a washing buffer solution. The kit for detecting whether or not to expose nonylphenol.
서열번호 1 내지 123으로 기재되는 각각의 유전자에 상보적이고 유전자를 증폭할 수 있는 프라이머 쌍을 모두 포함하는 노닐페놀 노출 여부 검출용 키트.

A kit for detecting exposure to nonylphenol comprising all of primer pairs complementary to each gene described in SEQ ID NOS: 1 to 123 and capable of amplifying a gene.

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