KR101626460B1 - Separating apparatus using column chromatography having remote control function and the control method thereof - Google Patents
Separating apparatus using column chromatography having remote control function and the control method thereof Download PDFInfo
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- KR101626460B1 KR101626460B1 KR1020150042129A KR20150042129A KR101626460B1 KR 101626460 B1 KR101626460 B1 KR 101626460B1 KR 1020150042129 A KR1020150042129 A KR 1020150042129A KR 20150042129 A KR20150042129 A KR 20150042129A KR 101626460 B1 KR101626460 B1 KR 101626460B1
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
- F16K—VALVES; TAPS; COCKS; ACTUATING-FLOATS; DEVICES FOR VENTING OR AERATING
- F16K31/00—Actuating devices; Operating means; Releasing devices
- F16K31/02—Actuating devices; Operating means; Releasing devices electric; magnetic
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/16—Injection
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/62—Detectors specially adapted therefor
- G01N30/74—Optical detectors
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- G—PHYSICS
- G05—CONTROLLING; REGULATING
- G05B—CONTROL OR REGULATING SYSTEMS IN GENERAL; FUNCTIONAL ELEMENTS OF SUCH SYSTEMS; MONITORING OR TESTING ARRANGEMENTS FOR SUCH SYSTEMS OR ELEMENTS
- G05B11/00—Automatic controllers
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- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06T1/00—General purpose image data processing
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- H—ELECTRICITY
- H04—ELECTRIC COMMUNICATION TECHNIQUE
- H04Q—SELECTING
- H04Q9/00—Arrangements in telecontrol or telemetry systems for selectively calling a substation from a main station, in which substation desired apparatus is selected for applying a control signal thereto or for obtaining measured values therefrom
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- Analytical Chemistry (AREA)
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- Life Sciences & Earth Sciences (AREA)
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- General Health & Medical Sciences (AREA)
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- Automation & Control Theory (AREA)
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- Treatment Of Liquids With Adsorbents In General (AREA)
Abstract
Description
More particularly, the present invention relates to a separation device using a column chromatography capable of remote control and a control method thereof. More particularly, the present invention relates to a separation device and a control method thereof using a remote-controllable column chromatographic method using a wired / wireless user terminal including a smart phone or a tablet PC The present invention relates to a separation apparatus using a column chromatography which can be remotely controlled and a control method thereof.
Column chromatography is a kind of chromatography that has been widely used for a long time. It is a method of filling a tubular container with a suitable filler to be a stationary phase uniformly, A sample is injected and a suitable solvent (eluent) is used as a mobile phase to separate the components.
The separation of the mixed sample through the column chromatography is performed for several hours. For this purpose, it is inconvenient for the user to manually perform the appropriate control manually while observing the apparatus on which the column chromatography is performed there was.
For example, in order to store a sample discharged with a solvent for separation in a column chromatography column tube, a discharged sample may be replaced with a sample storage tank storing a sample every time the sample is stored, In order to control the supply of the sample to the chromatographic column (container) or to control the operation of the valve installed at the site where the sample is discharged from the column tube, the user directly sees the device on which the column chromatography is performed by the eye There was a need to perform the necessary control.
In order to solve such a problem, in order to automate the separation method of a sample using the column chromatography, Korean Patent Laid-Open No. 10-2012-0005318 (published on 2012.01.06) discloses an elution solution of column chromatography Discloses a method for detecting a substance by attaching various measurement sensors such as Mass, UV Visible, and IR spectrometer to the portion where it appears. In Korean Patent Application Publication No. 2000-0010983 (published on January 16, 2012) An electrochemical detector for liquid chromatography, which detects electrochemically the separation state of a sample to be analyzed by using an electrode reaction, and a liquid chromatography apparatus using the detector.
However, in the case of using the above-described conventional techniques, it is not economical to use a very expensive equipment, and the worker has to work directly in the space where the detecting equipment is located, so that the inconvenience that the degree of freedom of the worker is limited is still Have.
Therefore, in separating the coloring material, it is possible to reduce the production cost by improving the economical efficiency by using an inexpensive apparatus, eliminating the inconvenience that the operator always has to reside near the column chromatography operator, There is a continuing need for the research and development of a method capable of controlling for the monitoring and separation of water.
SUMMARY OF THE INVENTION The present invention has been made to solve the above problems and it is an object of the present invention to provide a method and apparatus for monitoring a state of a separation apparatus using a column chromatography at a remote place by using a user terminal including a smart phone or a tablet PC, The present invention has been made to solve the above-mentioned problems, and it is an object of the present invention to provide a separation apparatus using column chromatography which can control the operation of the separation apparatus using the chromatography.
It is another object of the present invention to improve convenience for a user by providing various sensors in the column chromatography so that a predetermined operation is performed based on information sensed by the sensor even if the user does not directly control the sensor.
A separation apparatus using a column chromatography capable of remote control according to an embodiment of the present invention includes a column chromatography execution unit capable of separating a sample mixture through column chromatography and storing separated samples, respectively; An image information collecting unit including at least one camera for photographing separated images of the column chromatography performing unit in real time; A communication interface which is connected to a user terminal through a wired / wireless communication network and performs an interface relating to control and monitoring of the column chromatography performing unit and the image information collecting unit; And a controller for controlling the operation of the column chromatography unit based on the control signal received at the communication interface.
In addition, in the separation apparatus using a column chromatography capable of remote control according to an embodiment of the present invention, the column chromatography performing section includes a column tube in which column chromatography is performed, And an electronic control valve that is operated according to a control signal of the control unit is provided at a lower end of the column tube to be discharged.
In addition, in the separation apparatus using a column chromatography capable of remote control according to an embodiment of the present invention, the column chromatography performing unit may include a solvent supply unit for storing and supplying a solvent required to perform the separation of the sample mixture in the column tube Wherein the solvent supply unit is provided with an electronically controlled pump operated according to a control signal of the control unit, whereby solvent is supplied to the column tube.
Also, in the separation apparatus using a column chromatography capable of remote control according to an embodiment of the present invention, the column chromatography performing unit includes a sample storage unit for storing a sample separated and discharged from the column tube, The sample storage unit comprises a plurality of sample storage tanks, and the sample is separated by storing the sample discharged from the column tube sequentially to each sample storage tank according to the control signal of the control unit.
In addition, in the separation apparatus using a column chromatography capable of remote control according to an embodiment of the present invention, each of the sample storage tanks further includes a volume level sensor, and the sample discharged from the column tube is supplied to a sample storage tank The volume level sensor senses it and transmits it to the control unit so that the control unit automatically replaces the sample storage tank with the next sample storage tank.
According to another aspect of the present invention, there is provided a separation apparatus using a column chromatography capable of remote control, wherein the image information collecting unit recognizes a color of a sample extracted from a column chromatography performing unit based on an image input to a camera The sample color recognizing unit may further include a sample color recognizing unit for automatically changing the sample storage tank when the color of the portion having a specific range on the lower side of the column is different from the color inputted in advance .
In addition, a separation apparatus using a column chromatography capable of remote control according to an embodiment of the present invention includes a manual control mode in which a user manually controls a column chromatography performing unit and an image information collecting unit using a user terminal; A first automatic control mode for controlling the column chromatography performing unit every time a predetermined amount of sample is extracted using the volume level sensor of the sample storage unit; And a second automatic control mode for automatically controlling the column chromatography performing unit based on the color information of the sample recognized by the sample color recognizing unit. The user terminal can set at least one of the modes.
In addition, in the separation apparatus using column chromatography capable of remote control according to an embodiment of the present invention, the user terminal includes a smart device including a smart phone and a tablet PC; And a remote control dedicated user terminal for the separation device using the chromatography; , And the like.
The present invention also provides a method for separating a sample mixture of a separation apparatus using a remote controllable column chromatography, the method comprising: collecting a separated image of a column chromatography performing unit using at least one camera in real time, A video transmission step for transmitting the video data to the terminal; A control signal transmitting step of transmitting a control signal for the column chromatography performing unit and the image information collecting unit to a separating apparatus using column chromatography in a user terminal according to an image transmitted by the image transmitting step; And a control step of controlling operations of the column chromatography performing unit and the image information collecting unit according to the control signal received from the user terminal. The present invention also provides a method of separating a sample mixture using a remote controllable column chromatography.
Further, in the method of separating a sample mixture of a separation apparatus using a column chromatography capable of remotely controlling according to another embodiment of the present invention, the control step may include a step of providing a sample at a lower end of the column tube through which the sample separated through the column chromatography is discharged An electronic control valve; A solvent supplier for storing and supplying the developing solvent to the column tube; And a sample storage portion for storing the sample separated and extracted from the column tube; The control unit controls the operation of any one selected from the group consisting of:
According to another aspect of the present invention, there is provided a method of separating a sample mixture of a separation apparatus using a column chromatography capable of remote control, wherein the control step comprises a column chromatography execution unit The control unit automatically controls the operation of the information collecting unit so that the color of the portion having a specific range at the lower end of the column tube is different from the color inputted in advance based on the image input to the camera, Is replaced.
A remote controllable separation apparatus using column chromatography and a control method therefor according to the present invention can perform separation by using a column chromatography using at least one camera in real time in performing column chromatography, By transmitting the data to the user terminal, the user can monitor the separation process and the result of the column chromatography at a remote site, and the user performs column chromatography directly at the remote site using the user terminal based on the monitoring result By conventionally allowing the user to perform the column chromatography, the separation method using the column chromatography from the start to the end of the separation method using the column chromatography It is possible to solve the inconvenience that the user has to adhere to and improve the convenience of the user.
BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a diagram showing the overall configuration of a separation apparatus using a column chromatography capable of remote control according to an embodiment of the present invention. FIG.
2 is a block diagram showing a configuration of a column chromatography capable of remote control according to an embodiment of the present invention.
FIG. 3 is a schematic view of a remote controllable column chromatography according to an embodiment of the present invention.
4 is a flowchart for explaining a remote control method of a separation apparatus using column chromatography according to an embodiment of the present invention.
DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS The present invention will now be described in detail with reference to the accompanying drawings. While the invention is susceptible to various modifications and alternative forms, specific embodiments thereof are shown by way of example in the drawings and will herein be described in detail. It is to be understood, however, that the invention is not intended to be limited to the particular forms disclosed, but on the contrary, is intended to cover all modifications, equivalents, and alternatives falling within the spirit and scope of the invention.
Unless otherwise defined in this invention, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Terms such as those defined in commonly used dictionaries are to be interpreted as having a meaning consistent with the contextual meaning of the related art and are to be interpreted as either ideal or overly formal in the sense of the present application Do not.
BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a diagram showing the overall configuration of a separation apparatus using a column chromatography capable of remote control according to an embodiment of the present invention. FIG.
As shown in FIG. 1, the remote
The column chromatography performing unit 110 separates, extracts, and stores the mixed sample using the column chromatography. The image
The
The
That is, in the
The
Hereinafter, the control operation of the
The column chromatography performing unit 110 includes a
In this way, the user can control the point of time at which the separated sample is extracted by opening or closing the electronic control valve at the remote site through the
That is, according to the present invention, when the user monitors the discharge of the sample separated through the column chromatography through the
In addition, the
In the present invention, an electronic control pump is installed in the
That is, the present invention provides a method for supplying a developing solvent, which is a mobile phase, in a state in which a sample is loaded on a filler in a column tube 111 (that is, a separation process of column chromatography) Column chromatography can be carried out.
The
More specifically, the
The
The
As described above, the
The image
The camera includes an IP camera, and in order to realize multi-view on images of respective parts of the column chromatography performing unit 110, it is necessary to install and operate two or more cameras.
In addition, a camera having a zoom, pan, and tilt function may be installed so that the user can control the operation of the camera through the
The image of the execution part of the column chromatography taken by the image
The
The camera (IP camera) generally outputs compressed frames compressed by JPEG (Joint Photographic Expert Group) as IP packets. If necessary, the JPEG compressed image is converted into a video having a higher compression ratio such as H.264 And can be transmitted as a file or stream through the IP network.
At this time, the compression standard of the image compression stream is not limited to a specific standard, and a chunk is formed by segmenting a compressed file of a predetermined size, and the app installed in the
That is, the
The
In addition, in addition to the manual control mode in which the
For example, the
The image
The method for distinguishing the color of the sample extracted from the column color tube of the sample color recognizing unit is to compare the color of the portion having a specific range at the lower end of the column tube with the color previously inputted to the sample color recognizing unit, The method of recognizing the change of the extracted sample component is used.
In addition, a manual control mode for manually controlling the
That is, when the manual control mode and the first automatic control mode are selected at the same time, when the user does not input the control command on the
If the first automatic mode and the second automatic mode are selected at the same time, the sample storage tank is replaced according to a certain volume and color change of the sample to be automatically extracted without a user's control command.
FIG. 2 is a view for explaining a remote controllable column chromatography system according to an embodiment of the present invention.
As shown in FIG. 2, the separating
The present invention can develop a system for connecting a plurality of column chromatography-based separation apparatuses 100-n and a plurality of user terminals 10-n through the column
The transmission and reception of information between the separating apparatus using the column chromatography and the
FIG. 3 is a flowchart for explaining a control method of a separation apparatus using a column chromatography capable of remote control according to an embodiment of the present invention.
As shown in FIG. 3, the control method of the separation apparatus using the column chromatography, which can be remotely controlled, according to the present invention, first captures an image of the separation apparatus using column chromatography using at least one camera, To the user terminal in real time via the communication network (S101).
Thereafter, a control signal for the column chromatography performing unit and the image information collecting unit is transmitted from the user terminal to the separating apparatus using column chromatography according to the transmitted image (S102).
In accordance with the received control signal, the controller of the separation apparatus using the column chromatography controls operation of the column chromatography performing unit and the image information collecting unit (S103).
The operation of the column chromatography performing section is controlled by controlling the operation of the electronic control valve provided at the lower end from which the sample separated by the column chromatography is extracted, the solvent for storing and supplying the developing solvent (eluent) Controlling the operation of the feeder, controlling the operation of the sample reservoir to replace the sample reservoir containing the sample to be extracted separately from the column tube, and the like.
In order to control the operation of the column chromatography unit, if a sample extracted from the column tube is contained in the sample storage tank by a predetermined amount or more, the volume level sensor in the sample storage unit detects this and the control unit automatically replaces the sample storage tank , It is possible to automatically set the sample storage tank to be replaced when the color of the portion of the column tube at the lower end of the column is different from the previously input color based on the image input to the camera.
As described above, according to the present invention, a user monitors a separation device using column chromatography using a user terminal at a remote site, and controls operations necessary for direct column chromatography at a remote site using the user terminal based on the monitoring result The convenience of the user can be greatly improved by eliminating the inconvenience that the user has to stay near the separation device using the column chromatography until the column chromatography is completed when the user performs the column chromatography.
While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is clearly understood that the same is by way of illustration and example only and is not to be taken by way of limitation, I will understand. Accordingly, the technical scope of the present invention should be defined by the following claims.
10: User terminal
100: Separation device using column chromatography
110: column chromatographic performance unit
111: Column tube
112: solvent supply
113: sample storage unit
120: Image information collecting unit
121: camera
122: sample color recognition unit
130: Communication interface
140:
200: Server
Claims (11)
A column chromatographic performance unit capable of separating the sample mixture through column chromatography and storing each of the separated samples;
An image information collecting unit including at least one camera for photographing separated images of the column chromatography performing unit in real time;
A communication interface which is connected to a user terminal through a wired / wireless communication network and performs an interface relating to control and monitoring of the column chromatography performing unit and the image information collecting unit; And
And a control unit for controlling the operation of the column chromatography execution unit based on the control signal received at the communication interface.
Wherein the column chromatography performing section includes a column tube in which column chromatography is performed,
Wherein the column tube is provided with an electron control valve which is operated at a lower end of a column through which the separated sample is discharged in accordance with a control signal of the control unit.
Wherein the column chromatography performing unit further comprises a solvent supplier for storing and supplying the solvent required to perform the separation of the sample mixture in the column tube,
Wherein the solvent supply unit is provided with an electronic control pump operated according to a control signal of the control unit, whereby a solvent is supplied to the column tube.
Wherein the column chromatography execution unit includes a sample storage unit for storing a sample separated and discharged from the column tube,
Wherein the sample storage part comprises a plurality of sample storage tanks and samples are discharged by sequentially storing the samples discharged from the column tubes in the respective sample storage tanks according to the control signal of the controller. Separation device using chromatography.
Each of the sample reservoirs further comprising a volume level sensor,
Wherein the volume level sensor detects the volume of the sample discharged from the column tube in the sample storage tank and transfers the sample to the control unit so that the control unit automatically replaces the sample storage tank with the next sample storage tank. Separation device using column chromatography possible.
The image information collecting unit may further include a sample color recognizing unit that recognizes the color of the sample extracted from the column chromatography performing unit based on the image input to the camera,
Wherein the sample color recognizing unit automatically changes the sample storage tank when the color in the portion having a specific range at the lower end of the column tube is different from the previously inputted color. Separation device using column chromatography.
The separation apparatus using the column chromatography may include a manual control mode in which a user manually controls a column chromatography performing unit and an image information collecting unit using a user terminal;
A first automatic control mode for controlling the column chromatography performing unit every time a predetermined amount of sample is extracted using the volume level sensor of the sample storage unit; And
A second automatic control mode for automatically controlling the column chromatography performing unit based on the color information of the sample recognized by the sample color recognizing unit; Wherein the at least one mode is set through a user terminal.
The user terminal includes a smart device including a smart phone and a tablet PC; And
A remote control dedicated user terminal for the separation device using the chromatography; Wherein the column chromatography is performed by using a column chromatography.
An image transmission step of collecting separated images of the column chromatography performing unit using at least one camera in real time and transmitting the separated images to a user terminal through a wired / wireless communication network;
A control signal transmitting step of transmitting a control signal for the column chromatography performing unit and the image information collecting unit to a separating apparatus using column chromatography in a user terminal according to an image transmitted by the image transmitting step; And
And controlling the operation of the column chromatography performing unit and the image information collecting unit according to the control signal received from the user terminal. The remote control method according to claim 1, .
Wherein the control step includes an electronic control valve installed at a lower end of a column through which a sample separated through column chromatography is discharged; A solvent supplier for storing and supplying the developing solvent to the column tube; And a sample storage portion for storing the sample separated and extracted from the column tube; The method comprising the steps of: a) separating the sample mixture into a plurality of samples;
Wherein the controlling step controls the operation of the column chromatography performing unit and the image information collecting unit according to the control signal received from the user terminal,
Wherein the control unit automatically changes the sample storage tank when the color of the portion having a specific range on the lower side of the column tube is different from the color inputted in advance based on the image input to the camera, A method for separating a sample mixture of a separation apparatus using a column chromatography capable of performing a column chromatography.
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR102263055B1 (en) * | 2021-02-24 | 2021-06-09 | (주)아주과학 | Apparatus and method for analyzing amino acids |
WO2023080351A1 (en) * | 2021-11-05 | 2023-05-11 | 주식회사 엑소퍼트 | Automatic liquid fractionation apparatus for column chromatography |
JP7496901B2 (en) | 2021-11-05 | 2024-06-07 | エクソパート コーポレーション | Automated liquid fractionator for column chromatography. |
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KR20000010983A (en) | 1997-03-14 | 2000-02-25 | 겜마 아키라 | Electric-chemistry detector and apparatus for liquid chromatography and analysis method using thereof |
JP2000266751A (en) * | 1999-03-15 | 2000-09-29 | Matsushita Electric Ind Co Ltd | Immunochromatography measurement device |
KR20020083653A (en) * | 2001-04-27 | 2002-11-04 | 조수동 | UV-cloumn chromatography |
KR20050071572A (en) * | 2002-11-19 | 2005-07-07 | 하마마츠 포토닉스 가부시키가이샤 | Coloration measuring device |
KR20120005318A (en) | 2010-07-08 | 2012-01-16 | 삼성전기주식회사 | High temperature chromatography apparatus and method thereof |
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Publication number | Priority date | Publication date | Assignee | Title |
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KR102263055B1 (en) * | 2021-02-24 | 2021-06-09 | (주)아주과학 | Apparatus and method for analyzing amino acids |
WO2023080351A1 (en) * | 2021-11-05 | 2023-05-11 | 주식회사 엑소퍼트 | Automatic liquid fractionation apparatus for column chromatography |
JP7496901B2 (en) | 2021-11-05 | 2024-06-07 | エクソパート コーポレーション | Automated liquid fractionator for column chromatography. |
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