KR101456390B1 - Environment-Friendly preparation method of silver nanoparticle using turnip leaves - Google Patents
Environment-Friendly preparation method of silver nanoparticle using turnip leaves Download PDFInfo
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Abstract
Description
본 발명은 손쉽고 안전하며 효율좋게 은나노입자를 제조할 수 있는, 순무청을 이용한 은나노입자의 친환경적인 제조방법에 관한 것이다.The present invention relates to an eco-friendly method for manufacturing silver nanoparticles using pure silver, which can easily, safely and efficiently produce silver nanoparticles.
은나노입자는 소독용 살균수, 무좀약, 아토피 치료제, 기능성 항균치약, 항균연고 등과 같은 의약품, 양말, 셔츠, 스포츠 의류, 작업복, 환자복, 담요, 커튼, 부직포 등과 같은 섬유의 항균 처리, 스킨, 로션, 에센스, 아이크림, 영양크림, 립스틱 등과 같은 화장품류, 물티슈, 생리대, 기저귀, 젖병 및 세정제, 여성 청결제 등과 같은 위생용품, 주방용품, 욕실용품, 물병, 물컵 등의 가정용품, 휴대폰, 노트북, 정수기, 냉장고, 에어컨, 전화기, 에어크리너 등과 같은 가전제품의 내외장제, 타일, 벽지, 인조대리석, 외장용 도료 등과 같은 건축제품, 토양 살균제, 무농약 살균수 등과 같은 농업용품, 항균 배관, 살균 정수처리시스템 등과 같은 산업용품에 이르기까지 매우 다양한 분야에서 사용되고 있다.The silver nanoparticles can be used for antimicrobial treatment of fibers such as disinfecting water for disinfection, athlete's foot medicine, atopic medicine, functional antibacterial toothpaste, antibacterial ointment, etc., socks, shirts, sports clothes, work clothes, patient clothes, blankets, curtains and nonwoven fabrics, Such as toiletries, toiletries, water bottles, water cups, housewares such as toilet paper, cell phones, notebooks, etc., such as toiletries, essences, eye creams, nourishing creams, lipsticks and the like, wipes, sanitary napkins, diapers, Agricultural products such as interior and exterior materials for household appliances such as water purifier, refrigerator, air conditioner, telephone, air cleaner, agricultural products such as tiles, wallpaper, artificial marble, exterior paint, agricultural products such as soil disinfectant and pesticidal water, antibacterial piping, And so on.
이러한 은나노입자는 종래의 벌크 재료 제조방법으로는 제조될 수 없기 때문에, 화학적인 방법으로서 은 나노입자의 원료가 되는 질산은을 용해한 수용액에, 분산제, 소포제, 환원제 등을 함께 첨가하여 환원제에 의한 은의 환원작용을 유도하여 미세한 나노입자를 얻는 방법이 알려져 있다. 그러나, 이러한 방법은 그 과정이 복잡하며, 여러가지 재료가 다량 사용되기 때문에 부산물이 다량 발생될 뿐만 아니라 고비용이며 비효율적이라는 단점이 있다.Since these silver nanoparticles can not be manufactured by conventional bulk material manufacturing methods, a chemical agent such as a dispersant, defoaming agent, and a reducing agent are added together with an aqueous solution of silver nitrate, which is a raw material of silver nanoparticles, A method of inducing action to obtain fine nanoparticles is known. However, this method has a complicated process, and since a large amount of various materials are used, not only a large amount of by-products are produced, but also a high cost and inefficiency.
한편, 대한민국 공개특허 제2009-0021954호에는 정제수에 은염 수용액과 제1환원제 수용액이 혼합된 혼합액을 교반시키고, 별도의 제2환원제 수용액을 첨가한 다음 안정화제를 더 포함시켜 혼합하는, 은나노입자 콜로이드의 제조방법 및 이에 따른 은나노입자 콜로이드를 제공한다. 그러나, 상기 방법은 수소붕소화나트륨(sodium borohydride)와 같은 환원용 화합물을 사용하므로, 환경친화적이지 못한 한계가 있다.Korean Patent Laid-Open Publication No. 2009-0021954 discloses a silver nanoparticle colloid in which purified water is mixed with a mixed solution of a silver salt aqueous solution and a first reducing agent aqueous solution, an additional second reducing agent aqueous solution is added, And a silver nanoparticle colloid therefrom. However, since the above method uses a reducing compound such as sodium borohydride, it is not environmentally friendly.
따라서, 여전히 보다 손쉽고 안전하며 효과적이면서도 환경친화적으로 은나노입자를 제조할 수 있는 방법이 필요한 실정이다.Therefore, there is still a need for a method that can produce silver nanoparticles more easily, safely, effectively and environmentally friendly.
이에, 본 발명자들은 별도의 환원용 화합물을 사용하지 않고, 순무청(turnip leaves)을 이용하여 은나노입자를 환경친화적으로 합성할 수 있다는 것을 밝혀내어 본 발명을 완성한 것이다.Thus, the present inventors have found that silver nanoparticles can be eco-friendly synthesized by using turnip leaves without using a separate reducing compound, thereby completing the present invention.
본 발명의 목적은 손쉽고 안전하며 효율좋게 순무청을 이용한 은나노입자의 친환경적인 제조방법을 제공하는 데에 있다.It is an object of the present invention to provide an eco-friendly method of manufacturing silver nanoparticles using a net silver wire with ease, safety and efficiency.
상기 목적을 달성하기 위하여, 본 발명은 순무청 추출물을 제조하는 단계; 및 상기 순무청 추출물을 은염 화합물에 첨가한 후, 암흑 조건에서 반응시키는 단계를 포함하는, 순무청을 이용한 은나노입자의 제조방법을 제공한다.In order to achieve the above object, And a step of adding the extract to the silver salt compound and then reacting in a dark condition.
상기 순무청 추출물은 순무청을 80 내지 100℃의 수욕 상에서 2 내지 4 시간 동안 추출하여 제조할 수 있다. The extract can be prepared by extracting the extract from a water bath at 80 to 100 ° C for 2 to 4 hours.
상기 은염 화합물은 질산은(AgNO3), 과염소산은(AlClO4), 염소산은(AgClO3), 탄산은(Ag2CO3), 황산은(Ag2SO4), 염화은(AgCl), 브롬화은(AgBr), 초산은 및 불소은(AgF)으로 이루어진 군에서 선택된 어느 하나일 수 있다.The silver halide compounds are silver nitrate (AgNO 3 ), perchloric acid silver (AlClO 4 ), silver chlorate silver (AgClO 3 ), silver carbonate silver (Ag 2 CO 3 ), silver sulfate silver (Ag 2 SO 4 ) ), Silver nitrate and fluorine silver (AgF).
상기 순무청 추출물을 은염 화합물에 첨가한 후, 15 내지 40℃의 암흑 조건에서 2 내지 8시간 동안 반응시킬 수 있다. After the pure extract is added to the silver salt compound, the reaction can be carried out for 2 to 8 hours under dark condition at 15 to 40 ° C.
본 발명에 따라 제조된 은나노입자는 평균 직경이 10 내지 50 nm일 수 있다.The silver nanoparticles prepared according to the present invention may have an average diameter of 10 to 50 nm.
상기 은나노입자는 전나무조개버섯(Gloeophyllum abietinum), 갈색부후균(Gloeophyllum trabeum), 체토미움 글러브섬(Chaetomium globosum) 및 유색고약버섯(Phanerochaete sordida)으로 이루어진 군에서 선택된 목재부후균에 대한 항균활성을 나타낸다.The silver nanoparticles exhibit antimicrobial activity against wood rot fungi selected from the group consisting of Gloeophyllum abietinum, Gloeophyllum trabeum, Chaetomium globosum and Phanerochaete sordida .
본 발명은 상기 제조방법에 의해 얻어진 은나노입자를 함유하는 목재부후균 항균용 조성물을 제공한다.The present invention provides a composition for anti-microbial activity of wood containing silver nanoparticles obtained by the above production method.
상기 목재부후균은 전나무조개버섯(Gloeophyllum abietinum), 갈색부후균(Gloeophyllum trabeum), 체토미움 글러브섬(Chaetomium globosum) 및 유색고약버섯(Phanerochaete sordida)으로 이루어진 군에서 선택될 수 있다.The wood rot fungus may be selected from the group consisting of Gloeophyllum abietinum, Gloeophyllum trabeum, Chaetomium globosum and Phanerochaete sordida.
본 발명에 따르면, 순무청을 이용하여 짧은 시간 내 신속하게 독성 화학물질의 사용없이 나노크기의 은나노입자를 효율좋고 환경친화적으로 제조할 수 있으며, 특히 상기 은나노입자는 목재부후균에 대한 탁월한 항균활성을 나타낼 수 있다.According to the present invention, it is possible to produce nano-sized silver nanoparticles efficiently and environmentally friendly without using toxic chemicals in a short period of time by using pure silver. Particularly, the silver nanoparticles have excellent antimicrobial activity against wood- Lt; / RTI >
도 1은 본 발명의 일실시예에 따른 은나노입자의 UV-Vis 분광 분석 결과이고(삽입도는 질산은 용액에 대한 최대 흡강도와 반응시간 간의 상관관계를 나타낸 그래프),
도 2는 본 발명의 일실시예에 따른 은나노입자의 X선 회절(XRD) 분석 결과이고,
도 3은 본 발명의 일실시예에 따른 은나노입자의 동적 광산란(DLS) 분석 결과이고,
도 4는 본 발명의 일실시예에 따른 은나노입자의 푸리에 변환 적외선(FTIR) 분석 결과이고(A: 순무청 추출물, B: 은나노입자),
도 5는 본 발명의 일실시예에 따른 은나노입자의 다양한 목재부후균에 대한 항균 활성을 나타낸 한천확산시험 결과이고,
도 6은 본 발명의 일실시예에 따른 은나노입자의 다양한 목재부후균에 대한 항균 활성을 나타낸 현미경 분석 결과이다.FIG. 1 is a result of UV-Vis spectroscopic analysis of silver nanoparticles according to an embodiment of the present invention (the degree of insertion is a graph showing a correlation between a maximum absorption intensity and a reaction time for a silver nitrate solution)
2 is a result of X-ray diffraction (XRD) analysis of silver nanoparticles according to an embodiment of the present invention,
3 is a result of dynamic light scattering (DLS) analysis of silver nanoparticles according to an embodiment of the present invention,
FIG. 4 is a Fourier transform infrared (FTIR) analysis result of silver nanoparticles according to an embodiment of the present invention (A: net extract, B: silver nanoparticles)
FIG. 5 shows the results of an agar diffusion test showing antimicrobial activity against various wood rot fungi of silver nanoparticles according to an embodiment of the present invention,
FIG. 6 is a microscopic analysis showing the antimicrobial activity of various wood rot fungi of silver nanoparticles according to an embodiment of the present invention.
이하, 본 발명을 보다 상세하게 설명한다.Hereinafter, the present invention will be described in more detail.
본 발명에서는 별도의 환원용 화합물을 사용하지 않고, 순무청(turnip leaves)을 이용하여 은나노입자를 환경친화적으로 합성할 수 있다는 것을 밝혀내었고, 특히 순무청을 이용하여 은나노입자를 제조할 경우 항균 활성을 갖는 은나노입자가 균일한 나노 크기로 분포하는 것을 확인하여 본 발명을 완성하였다.In the present invention, it has been revealed that silver nanoparticles can be environmentally synthesized using turnip leaves without using a separate reducing compound, and in particular, when silver nanoparticles are prepared using pure silver nanoparticles, The present inventors completed the present invention by confirming that silver nanoparticles having an activity are distributed in a uniform nano size.
보다 상세하게는, 순무청 추출물을 은염 예를들어, 질산염에 첨가하여 반응시킨 후, 도 1과 같이 4시간 만에 은나노입자가 포화상태로 생성됨을 확인할 수 있으며, 도 2와 같이 면심입방구조-지향 결정성 은나노입자의 형성을 XRD 패턴으로부터 확인할 수 있었다. 또한, 도 3과 같이 동적 광산란(DLS) 분석 결과로부터 은나노입자와 순무청 추출물의 생리활성분자 간의 상호작용을 일으키는 관능기를 확인하였고, 은나노입자의 제타전위는 pH 7.5에서 -14.8 mV로 나타났다.More specifically, it can be confirmed that the silver nanoparticles are formed in a saturated state in 4 hours as shown in FIG. 1 after adding the silver manganese extract to the silver salt, for example, the nitrate salt. As shown in FIG. 1, The formation of oriented crystalline silver nanoparticles could be confirmed from the XRD patterns. Also, as shown in FIG. 3, the functional groups causing the interaction between the physiologically active molecules of the silver nanoparticles and the net extracts of silkworm were identified from the results of dynamic light scattering (DLS) analysis, and the zeta potential of the silver nanoparticles was -14.8 mV at pH 7.5.
그리고, 도 4는 은나노입자의 FTIR 분석 결과(B)로서, 순무청 추출물(A)과 비교할 때 은과 결합에 의해 더 높은 주파수로 영역 이동이 일어났으며, 도 5 및 도 6과 같이 본 발명에 따른 은나노입자는 (a) 전나무조개버섯(Gloeophyllum abietinum), (b) 갈색부후균(Gloeophyllum trabeum), (c) 체토미움 글러브섬(Chaetomium globosum) 및 (d) 유색고약버섯(Phanerochaete sordida)의 목재부후균에서 모두 우수한 항균활성을 나타내었다.FIG. 4 shows the FTIR analysis result (B) of the silver nanoparticles. As compared with the extract (A), the region shifted to the higher frequency by silver bonding, and as shown in FIG. 5 and FIG. 6, The silver nanoparticles according to (a) Gloeophyllum abietinum, (b) Gloeophyllum trabeum, (c) Chaetomium globosum and (d) Phanerochaete sordida The antimicrobial activity of the woody rot fungi was excellent.
따라서, 본 발명에서는 어떠한 다른 독성 화합물이나 기술적으로 고비용의 공정을 이용하지 않고 순무청을 이용하여 은나노입자를 환경친화적이면서도 손쉽게 제조할 수 있는 최초의 발명이다.Therefore, in the present invention, it is the first invention that can easily produce the silver nanoparticles in an environmentally friendly manner by using the pure silver powder without using any other toxic compound or a technically expensive process.
또한, 본 발명에서는 상기 제조방법에 의해 얻어진 은나노입자를 함유하는 목재부후균 항균용 조성물을 제공한다.Further, the present invention provides a composition for antifungal antifungal of wood containing silver nanoparticles obtained by the above production method.
상기 조성물은 소독용 살균수, 무좀약, 아토피 치료제, 기능성 항균치약, 항균연고 등과 같은 의약품; 양말, 셔츠, 스포츠 의류, 작업복, 환자복, 담요, 커튼, 부직포 등과 같은 섬유의 항균 처리; 스킨, 로션, 에센스, 아이크림, 영양크림, 립스틱 등과 같은 화장품류; 물티슈, 생리대, 기저귀, 젖병 및 세정제, 여성 청결제 등과 같은 위생용품; 주방용품, 욕실용품, 물병, 물컵 등의 가정용품; 휴대폰, 노트북, 정수기, 냉장고, 에어컨, 세탁기, 전화기, 에어크리너 등과 같은 가전제품의 내외장제; 타일, 벽지, 인조대리석, 외장용 도료 등과 같은 건축제품; 토양 살균제, 무농약 살균수 등과 같은 농업용품; 항균 배관; 살균 정수처리시스템 등과 같은 산업용품에 이르기까지 매우 다양한 분야에서 사용될 수 있다. 특히, 에어컨, 세탁기 등과 같은 살균이 필요한 전자제품, 옷, 신발 등과 같은 의류, 잡화에 사용되는 섬유의 항균 처리에 사용될 수 있다.
The composition may be used as disinfecting water for disinfection, athlete's foot medicine, atopic treatment agent, functional antibacterial toothpaste, antibacterial ointment and the like; Antibacterial treatment of fibers such as socks, shirts, sportswear, work clothes, hospital gowns, blankets, curtains, non-woven fabrics; Cosmetics such as skin, lotion, essence, eye cream, nutritional cream, lipstick and the like; Sanitary articles such as wet tissues, sanitary napkins, diapers, baby bottles and cleaners, and female cleaners; Household appliances such as kitchen utensils, bathroom utensils, water bottles, water cups; In and out of home appliances such as cell phones, laptops, water purifiers, refrigerators, air conditioners, washing machines, telephones, air cleaners and the like; Architectural products such as tiles, wallpaper, artificial marble, exterior paint; Agricultural products such as soil-disinfecting agents, non-pesticidal water; Antibacterial piping; Sterilizing water treatment systems, and the like. Especially, it can be used for antimicrobial treatment of clothes such as electronic products, clothes, shoes, and the like, which are required to be sterilized, such as air conditioners and washing machines.
이하, 하기 실시예에 의해 본 발명을 보다 상세하게 설명한다. 그러나, 하기 실시예는 본 발명의 내용을 구체화하기 위한 설명일 뿐 실시예에 의해 본 발명이 한정되는 것은 아니다. Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the following examples are intended only to illustrate the present invention and are not intended to limit the scope of the present invention.
<실시예 1> 순무청을 이용한 은나노입자 제조Example 1 Production of silver nanoparticles using pure water
1. 순무청 추출물 준비1. Preparation of net extract
순무청 30g을 멸균 증류수에서 세정한 후, 세절하였으며, 100 mL 탈이온수에 첨가하고 30분 동안 90℃ 수욕상에서 유지시켜 순무청 추출물을 준비하였다. 상기 추출물을 여과한 후, 4℃에서 보관하여 이후 실험에 사용하였다. 30 g of pure water was washed in sterilized distilled water, and then it was sieved, added to 100 mL of deionized water, and kept in a water bath at 90 ° C for 30 minutes to prepare a pure water extract. The extract was filtered and stored at 4 ° C for further experiments.
2. 은나노입자 제조2. Manufacturing of silver nanoparticles
상기 준비된 순무청 추출물 1 mL를 1 mM AgNO3 5 mL에 첨가한 후, 25℃, 암조건 하에서 반응시켜 은나노입자를 제조하였다.1 mL of the prepared extract was added to 5 mL of 1 mM AgNO 3 and reacted at 25 ° C under dark conditions to prepare silver nanoparticles.
<실시예 2> 은나노입자의 성능 평가≪ Example 2 > Performance evaluation of silver nanoparticles
1) UV-Vis 분광 분석1) UV-Vis spectroscopy
UV-Vis 분광 분석은 나노입자의 형상과 안정성을 검토하기 위한 중요한 기술로서, 실시예 1에 따른 은나노입자의 UV-Vis 분광 분석을 300 nm 내지 1100 nm에서 Beckman Coulter (Model: DU-730)을 이용하여 수행하였다.UV-Vis spectroscopic analysis is an important technique for examining the shape and stability of nanoparticles. UV-Vis spectroscopic analysis of silver nanoparticles according to Example 1 was performed using a Beckman Coulter (Model: DU-730) at 300 nm to 1100 nm .
그 결과, 4시간 후 용액의 색이 황갈색으로 변화하여 은나노입자의 생성을 확인할 수 있었으며, 특히 도 1은 반응시간에 따라 형성된 은나노입자의 UV-Vis 분광 분석 결과로서, 약 390 nm에서의 표면 플라즈몬 공명(SPR)의 형성이 반응 4시간 후 은나노입자의 형성이 포화에 도달하였음을 나타내었다. As a result, the color of the solution changed to yellowish brown after 4 hours and the formation of silver nanoparticles was confirmed. Particularly, FIG. 1 shows the result of UV-Vis spectroscopic analysis of silver nanoparticles formed according to the reaction time, The formation of resonance (SPR) showed that formation of the silver nanoparticles reached saturation after 4 hours of reaction.
2) X선 회절(XRD) 분석2) X-ray diffraction (XRD) analysis
실시예 1에 따른 은나노입자의 결정구조 및 격자의 셀 파라미터에 대한 정보를 얻기 위해, 2θ가 30° 내지 80°의 영역에서 Cu Kα1 (λ = 1.54178 Å)에 의해 XRD (PANalytical X'pert PRO MPD)를 이용하여 분석을 수행하였다. 이때, 은나노입자의 크기는 Debye-Scherrer 식[Annual Review of Analytical Chemistry 2, 435-462]을 이용하여 산출되었다.In order to obtain information on the crystal structure of the silver nanoparticles according to Example 1 and the cell parameters of the lattice, the XRD (PANalytical X'pert PRO MPD (XRD) by Cu K? 1 ) Were used for analysis. At this time, the size of the silver nanoparticles was calculated using the Debye-Scherrer formula [Annual Review of Analytical Chemistry 2, 435-462].
도 2와 같이, XRD 분석을 통해 은나노입자의 면심 입방구조(fcc)를 기준으로 (111), (200), (220), (311) 및 (222)의 Bragg 반사에 대응하는 강한 피크를 나타내었으며, Debye-Scherrer 식에 의해 미소결정 도메인의 크기가 ~20 nm이었다.2, XRD analysis showed strong peaks corresponding to Bragg reflections of (111), (200), (220), (311) and (222) on the basis of the face-centered cubic structure (fcc) of the silver nanoparticles And the size of the microcrystalline domains was ~ 20 nm by the Debye-Scherrer equation.
3) 동적 광산란(DLS) 및 제타 전위 측정3) Dynamic light scattering (DLS) and zeta potential measurement
실시예 1에 따른 은나노입자의 수력학적 직경과 제타 전위는 Malvern Zetasizer Nano (Malvern Instruments Ltd, UK)를 이용하여 동적 광산란(DLS)에 의해 분석하였다. The hydrodynamic diameter and zeta potential of the silver nanoparticles according to Example 1 were analyzed by dynamic light scattering (DLS) using a Malvern Zetasizer Nano (Malvern Instruments Ltd, UK).
도 3은 실시예 1에 따른 은나노입자의 동적 광산란 분석 결과로서, 39.5 nm의 주요피크 위치가 pH 7.5에서의 은나노입자의 측정된 수력학적 직경으로 확인되었고, 은콜로이드의 제타전위는 pH 7.5에서 -14.8 mV로 나타났다.FIG. 3 shows the result of dynamic light scattering analysis of silver nanoparticles according to Example 1, in which the main peak position of 39.5 nm was confirmed by the measured hydrodynamic diameter of silver nanoparticles at pH 7.5, and the zeta potential of silver colloid was at pH 7.5 - 14.8 mV, respectively.
4) 푸리에 변환 적외선(FTIR) 분석4) Fourier transform infrared (FTIR) analysis
FTIR 분석을 위하여, 실시예 1에 따른 은나노입자를 25,000 x g에서 20분 동안 원심분리한 후, 얻어진 침전을 탈이온수로 3회 세정하여 결합되지 않은 성분들을 제거하였다. 이렇게 정제된 시료를 건조시키고 KBr 분말과 갈아 Jasco FTIR 5300 분광분석기를 이용하여 분석하였다.For FTIR analysis, the silver nanoparticles according to Example 1 were centrifuged at 25,000 x g for 20 minutes, and the resulting precipitate was washed three times with deionized water to remove unbound components. The purified samples were dried and analyzed by KBr powder and ground using a Jasco FTIR 5300 spectrometer.
도 4는 실시예 1에 따른 은나노입자의 FTIR 분석 결과(B)로서, 순무청 추출물(A)과 비교할 때 은과 결합에 의해 더 높은 주파수로 영역 이동이 일어났다.FIG. 4 is a FTIR analysis result (B) of the silver nanoparticles according to Example 1, and region shift was performed at a higher frequency by silver bonding as compared with the extract of pure silver manganese (A).
5) 한천 확산법을 이용한 항균시험5) Antibacterial test using agar diffusion method
실시예 1에 따른 은나노입자의 목재부후균에 대한 항균 효과를 검토하기 위하여, 한천 확산법을 수행하였다. 즉, 목재부후균을 감자 덱스트로즈 한천 플레이트 가운데 상에 접종하고 30℃에서 3일동안 배양하였다. 균의 직경이 4-5 cm에 달할 때 다양한 농도의 은나노입자(1, 2 및 4 mg)로 디스크 로딩된 멸균 페이퍼를 균 주변 상에 위치시켰다. 균사체 성장이 대조군(순무청 추출물)을 함유한 주위 웰을 뒤덮을 때까지 상기 플레이트를 30℃에서 더 배양하였다. 이때, 사용된 목재부후균은 Gloeophyllum abietinum(KACC51949), G.trabeum(KACC43361), Chaetomium globosum(KACC42262) 및 Phanerochaete sordida(KACC43367)이었다.In order to examine the antimicrobial effect of the silver nanoparticles according to Example 1 on wood-borne microorganisms, the agar diffusion method was carried out. That is, the wood-debris was inoculated on a potato dextrose agar plate and cultured at 30 ° C for 3 days. When the diameter of the bacterium reached 4-5 cm, disk-loaded sterile paper was placed on the periplasm of the bacteria with varying concentrations of silver nanoparticles (1, 2 and 4 mg). The plate was further incubated at 30 DEG C until mycelial growth covered surrounding wells containing control (net extract). The wood rot fungi used were Gloeophyllum abietinum ( KACC51949), G.trabeum ( KACC43361), Chaetomium globosum (KACC42262) and Phanerochaete sordida (KACC43367).
도 5와 같이, 실시예 1에 따른 은나노입자는 (a) Gloeophyllum abietinum(KACC51949), (b) G.trabeum(KACC43361), (c) Chaetomium globosum(KACC42262) 및 (d) Phanerochaete sordida(KACC43367)의 목재부후균에서 우수한 항균활성을 나타내었다.As shown in FIG. 5, the silver nanoparticles according to Example 1 were synthesized from (a) Gloeophyllum abietinum ( KACC51949), (b) G.trabeum ( KACC43361), (c) Chaetomium globosum (KACC42262), and (d) Phanerochaete sordida It showed excellent antimicrobial activity in wood rot fungi.
6) 광학현미경 분석6) Optical microscope analysis
실시예 1에 따른 은나노입자를 광학현미경(Nikon Eclipse 80i, Tokyo, Japan) 하에서 관찰하였다. 이때, 4 mg 은나노입자와 배양한 균사체를 락토페놀 코튼 블루로 염색하고 형상 변화를 광학현미경으로 관찰하였다.The silver nanoparticles according to Example 1 were observed under an optical microscope (Nikon Eclipse 80i, Tokyo, Japan). At this time, 4 mg silver nanoparticles and mycelium cultured were stained with lactophenol cotton blue, and the shape change was observed with an optical microscope.
도 6에 도시된 바와 같이, 은나노입자를 처리한 균사는 팽창된 가장자리와 깨진 균사체를 갖는 균사체 변형이 관찰된 반면, 은나노입자를 처리하지 않은 대조군에서는 정상적인 가지 형태를 갖는 균사체를 관찰할 수 있었다.
As shown in Fig. 6, hyphae treated with silver nanoparticles showed mycelial deformation with expanded edges and broken mycelium, whereas normal mycelia with control groups without silver nanoparticles were observed.
이상과 같이, 본 발명은 비록 한정된 실시예와 도면에 의해 설명되었으나, 본 발명은 이것에 의해 한정되지 않으며 본 발명이 속하는 기술 분야에서 통상의 지식을 가진 자에 의해 본 발명의 기술 사상과 아래에 기재될 청구범위의 균등 범위 내에서 다양한 수정 및 변형이 가능함은 물론이다. While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments. It is to be understood that various modifications and changes may be made without departing from the scope of the appended claims.
Claims (8)
상기 순무청 추출물을 은염 화합물에 첨가한 후, 암흑 조건에서 반응시키는 단계
를 포함하는, 순무청을 이용한 은나노입자의 제조방법.Preparing an extract of Pseudomonas sp. And
Adding the pure extract to the silver salt compound, and then reacting in a dark condition
Wherein the silver nanoparticles are produced by a method comprising the steps of:
[Claim 7] The method according to claim 7, wherein the wood-rot fungus is selected from the group consisting of Gloeophyllum abietinum, Gloeophyllum trabeum, Chaetomium globosum and Phanerochaete sordida Wherein the antimicrobial activity of the wood-degrading antimicrobial agent is at least 10%
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| KR102292488B1 (en) | 2020-05-25 | 2021-08-24 | 대진대학교 산학협력단 | Manufacturing method of silver nanoparticles using citrus fruits |
| KR20230043576A (en) | 2021-09-24 | 2023-03-31 | 강원대학교산학협력단 | Silver nanoparticles using sedge flower extract and method for manufacturing the same |
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| KR102292488B1 (en) | 2020-05-25 | 2021-08-24 | 대진대학교 산학협력단 | Manufacturing method of silver nanoparticles using citrus fruits |
| KR20230043576A (en) | 2021-09-24 | 2023-03-31 | 강원대학교산학협력단 | Silver nanoparticles using sedge flower extract and method for manufacturing the same |
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