KR101440460B1 - Medium composition with improved sensitivity and selectivity for Campylobacter and preparation method thereof - Google Patents
Medium composition with improved sensitivity and selectivity for Campylobacter and preparation method thereof Download PDFInfo
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Abstract
본 발명은 캠필로박터에 대한 민감성 및 선택성을 개선한 배지 조성물 및 그 제조방법에 관한 것이다.The present invention relates to a culture medium having improved sensitivity and selectivity to camphilobacter and a method for producing the same.
Description
본 발명은 캠필로박터에 대한 민감성 및 선택성을 개선한 배지 조성물 및 그 제조방법에 관한 것이다.The present invention relates to a culture medium having improved sensitivity and selectivity to camphilobacter and a method for producing the same.
Campylobacter spp.로 인한 식중독 사고는 전세계적으로 발생되고 있으며, 특히 북미와 유럽 등의 선진국에서는 Salmonella와 더불어 가장 문제시되고 있다. 식품(주로 계육) 내 Campylobacter 검출 시, 국내외 공인 검출법에서는 증균 후, 선택배양을 통해 검출을 실시하고 있다. 가장 널리 사용되는 선택배지는 mCCDA 배지(modified charcoal cefoperazone deoxycholate agar)라는 혈액이 함유되지 않은 선택배지로서, 활성탄, sodium pyruvate, cefoperazone 등을 함유하여 사용하고 있다. 해당 선택배지는 FDA, ISO 등과 같은 국제적 검출기준에서 사용되고 있다. Food poisoning accidents caused by Campylobacter spp. It is occurring worldwide, especially in developed countries such as North America and Europe, with Salmonella being the most problematic. In the detection of Campylobacter in food (mainly chickens), in domestic and overseas certified detection methods, detection is carried out after enrichment and selective culture. The most widely used selective medium is a selective medium containing no blood, such as mCCDA (modified charcoal cefoperazone deoxycholate agar), which contains activated carbon, sodium pyruvate, and cefoperazone. The selected medium is used in international detection standards such as FDA, ISO, etc.
그러나 최근에는 계육(닭고기)에서 cephalosporin 계열의 항생제에 강한 내성을 보이는 세균이 증가하는 추세이다. 이러한 세균은 ESBL이라는 효소를 생산하게 되는데, 이 효소가 cephalosporin에 강한 내성을 보이게 한다. 주로 장내 세균과에서 이러한 현상을 보이며 그 중 E. coli가 대표적이다. However, recently, there has been an increase in the number of bacteria showing strong resistance to cephalosporin antibiotics in chicken meat. These bacteria produce an enzyme called ESBL, which is highly resistant to cephalosporin. Escherichia coli (E. coli) is the most prevalent in intestinal bacteria.
mCCDA를 비롯한 대부분의 캠필로박터 선택배지는 캠필로박터 외의 경쟁균의 배제를 위하여 cephalosporin 계열 항생제의 일종인 cefoperzone을 사용하게 되는데 이러한 경우 ESBL 생성 E. coli가 선택배지 위에서 높은 수준으로 자라 캠필로박터의 선택적인 구분을 어렵게 한다. 특히 전세계적으로 계육에서 ESBL 생성 E. coli가 증가하고 있어 선택배지 상에서 이에 대한 개선이 필요하다. Most campylobacter selection media, including mCCDA, use cefoperzone, a cephalosporin antibiotic, to exclude competitors other than Campylobacter. In this case, ESBL-producing E. coli grows high on the selective medium and it is difficult to selectively distinguish Campylobacter do. Especially, ESBL-producing E. coli in chicken meat is increasing worldwide, so it needs to be improved on selective medium.
관련 선행특허로 대한민국 특허공개번호 제1020090085202호는 캠필로박터균의 배양 또는 수송용 배지에 관한 것으로, (a) 캠필로박터(Campylobacter)균 성장 유지에 유효한 에너지원(energy source)을 포함하는 영양 배지(nutrient medium); (b) 혈액(blood) 또는 혈청(serum); 및 (c) 젤라틴(gelatin) 성분을 포함하는 캠필로박터(Campylobacter) 균의 배양(culture) 또는 수송(transport)용 배지 조성물이 기재되어 있으며,Korean Patent Publication No. 1020090085202 discloses a culture medium for the cultivation or transportation of Campylobacter bacteria. The culture medium comprises (a) a nutrient medium containing an energy source effective for growth of Campylobacter ); (b) blood or serum; And (c) a culture medium for the culture or transport of Campylobacter fungi comprising a gelatin component,
또 다른 선행특허로 대한민국 특허공개번호 제 1020080082561호는 식품 위해 미생물 검출용 프라이머 및 이를 이용한 식품 위해 미생물 검출방법에 관한 것으로, 해당 특허의 서열번호 19의 염기서열로 이루어지는 프라이머 및 서열번호 20의 염기서열로 이루어지는 프라이머로 이루어지고 Tm 값이 80.7℃인 303 bp의 캠필로박터 제주니 특이적 유전자 산물을 증폭시키는 것을 특징으로 하는 캠필로박터 제주니(Camphylobacter jejuni) 검출용 프라이머가 기재되어 있다.
As another prior patent, Korean Patent Publication No. 1020080082561 discloses a primer for detecting food harmful microorganisms and a method for detecting a food microorganism using the same, wherein the primer comprising the nucleotide sequence of SEQ ID NO: 19 and the nucleotide sequence of SEQ ID NO: 20 And a Tm value of 80.7 ° C. The primers for detection of Camphylobacter jejuni are also described.
본 발명은 상기의 문제점을 해결하고 상기의 필요성에 의하여 안출된 것으로서 본 발명의 목적은 개선된 민감도를 가지는 새로운 캠필로박터균 배지를 제공하는 것이다.Disclosure of Invention Technical Problem [8] The present invention has been accomplished to solve the above problems and to provide a novel Campylobacterium cell culture medium having improved sensitivity.
본 발명의 다른 목적은 개선된 선택성을 가지는 새로운 캠필로박터균 배지를 제공하는 것이다.It is another object of the present invention to provide a new camphor bacterium culture medium having improved selectivity.
본 발명의 또 다른 목적은 개선된 민감도를 가지는 새로운 캠필로박터균 배지 제조방법을 제공하는 것이다.It is still another object of the present invention to provide a novel method of producing Campylobacter culture medium having improved sensitivity.
본 발명의 또 다른 목적은 개선된 선택성을 가지는 새로운 캠필로박터균 배지 제조방법을 제공하는 것이다.It is still another object of the present invention to provide a novel method for producing Campylobacter culture medium having improved selectivity.
상기의 목적을 달성하기 위하여 본 발명은 옥소이드(OXOID) 사의 뉴트리언트 브로쓰 넘버 2, 세균 활성탄(Bacteriological charcoal), 카제인 가수분해물(Casein hydrolysate) , 디옥신콜린산 나트륨(Sodium desoxycholate),황산제일철(Ferrous sulphate), 피루브산 나트륨(Sodium pyruvate), 아가, 세포페라존(Cefoperazone),암포테리신(Amphotericin) B 및 포타슘 클라블라네이트를 포함하는 캠필로박터(Campylobacter)에 대한 민감성을 개선한 배지 조성물을 제공한다.In order to accomplish the above object, the present invention provides a pharmaceutical composition comprising a nutrient broth No. 2 of OXOID, a bacteriological charcoal, casein hydrolyzate, sodium desoxycholate, ( Campylobacter ) including Ferrous sulphate, Sodium pyruvate, Agar, Cefoperazone, Amphotericin B and Potassium clavulanate to provide a medium composition improved in sensitivity to Campylobacter do.
본 발명의 일 구현예에 있어서, 상기 조성물의 조성은 뉴트리언트 브로쓰 넘버 2 25 중량부, 세균 활성탄(Bacteriological charcoal) 4 중량부, 카제인 가수분해물(Casein hydrolysate) 3 중량부, 디옥신콜린산 나트륨(Sodium desoxycholate) 1 중량부,황산제일철(Ferrous sulphate) 0.25 중량부, 피루브산 나트륨(Sodium pyruvate) 0.25 중량부 및 아가 12 중량부 비율인 것이 바람직하나 이에 한정되지 아니한다.In one embodiment of the invention, the composition comprises 25 parts by weight of Nutrient Broth No. 2, 4 parts by weight of Bacteriological charcoal, 3 parts by weight of casein hydrolyzate, 3 parts by weight of sodium dioxincollate 1 part by weight of sodium desoxycholate, 0.25 parts by weight of ferrous sulphate, 0.25 parts by weight of sodium pyruvate and 12 parts by weight of agar.
또 본 발명은 옥소이드(OXOID) 사의 뉴트리언트 브로쓰 넘버 2, 세균 활성탄(Bacteriological charcoal), 카제인 가수분해물(Casein hydrolysate) , 디옥신콜린산 나트륨(Sodium desoxycholate),황산제일철(Ferrous sulphate), 피루브산 나트륨(Sodium pyruvate), 아가, 세포페라존(Cefoperazone),암포테리신(Amphotericin) B 및 포타슘 클라블라네이트를 첨가하는 단계를 포함하는 캠필로박터(Campylobacter)에 대한 민감성을 개선한 배지 조성물의 제조방법을 제공한다.The present invention also relates to a pharmaceutical composition comprising an active ingredient selected from the group consisting of Nutrient Broth No. 2 of OXOID, Bacteriological charcoal, Casein hydrolysate, Sodium desoxycholate, Ferrous sulphate, A method for preparing a medium composition having improved sensitivity to Campylobacter comprising the steps of adding sodium pyruvate, agar, Cefoperazone, Amphotericin B and potassium clavulanate, to provide.
또한 본 발명은 옥소이드(OXOID) 사의 뉴트리언트 브로쓰 넘버 2, 세균 활성탄(Bacteriological charcoal), 카제인 가수분해물(Casein hydrolysate) , 디옥신콜린산 나트륨(Sodium desoxycholate),황산제일철(Ferrous sulphate), 피루브산 나트륨(Sodium pyruvate), 아가, 세포페라존(Cefoperazone),암포테리신(Amphotericin) B 및 포타슘 클라블라네이트를 포함하는 캠필로박터(Campylobacter)에 대한 선택성을 개선한 배지 조성물을 제공한다.The present invention also relates to a pharmaceutical composition comprising an active ingredient selected from the group consisting of Nutrient Broth No. 2 of OXOID, Bacteriological charcoal, Casein hydrolyzate, Sodium desoxycholate, Ferrous sulphate, There is provided a medium composition improved in selectivity to Campylobacter including sodium pyruvate, agar, Cefoperazone, Amphotericin B and potassium clavulanate.
또한 본 발명은 옥소이드(OXOID) 사의 뉴트리언트 브로쓰 넘버 2, 세균 활성탄(Bacteriological charcoal), 카제인 가수분해물(Casein hydrolysate) , The present invention also relates to the use of Nutrient Broth No. 2 of OXOID, Bacteriological charcoal, casein hydrolyzate,
디옥신콜린산 나트륨(Sodium desoxycholate),황산제일철(Ferrous sulphate), Sodium desoxycholate, ferrous sulphate,
피루브산 나트륨(Sodium pyruvate), 아가, 세포페라존(Cefoperazone),암포테리신(Amphotericin) B 및 포타슘 클라블라네이트를 첨가하는 단계를 포함하는 캠필로박터(Campylobacter)에 대한 선택성을 개선한 배지 조성물의 제조방법을 제공한다.A method for producing a medium composition improved in selectivity to Campylobacter including the step of adding sodium pyruvate, agar, Cefoperazone, Amphotericin B and potassium clavulanate .
또 본 발명은 식품 샘플을 상기 본 발명의 배지 조성물에서 배양하고, 배양 후 캠필로박터를 확인 동정하는 단계를 포함하는 식품 샘플에서 캠필로박터를 확인 동정하는 방법을 제공한다.The present invention also provides a method for identifying and identifying a Campylobacter in a food sample comprising culturing a food sample in the culture composition of the present invention and identifying and identifying the Campylobacterium after culturing.
이하 본 발명을 설명한다.Hereinafter, the present invention will be described.
mCCDA 배지는 OXOID사의 Campylobacter Blood-Free Selective Agar Base CM0739(표 1) 및 CCDA Selective Supplement SR0155(표 2)로부터 제조될 수 있으며 그 각각의 조성은 아래의 표와 같다. The mCCDA medium can be prepared from OXOID's Campylobacter Blood-Free Selective Agar Base CM0739 (Table 1) and CCDA Selective Supplement SR0155 (Table 2), and their respective compositions are shown in the table below.
본 발명에서는 세계적으로 가장 널리 사용되는 고체배지인 mCCDA 배지에 ESBL inhibitor인 Clavulanic acid (혹은 potassium clavulanate)를 함유시켜 배지의 민감도와 선택성을 개선하였다. 해당 배지의 우수한 민감도과 선택성은 닭의 린스액(rinsate)에서 Clavulanic acid가 첨가되지 않은 일반 mCCDA 배지와 비교검증 하였다. 개발된 배지의 명칭은 C-mCCDA 배지 (Clavulanic acid B supplemented mCCDA)로 아래에서 칭한다.In the present invention, an ESBL inhibitor, clavulanic acid (or potassium clavulanate), was added to the mCCDA medium, which is the most widely used solid medium in the world, to improve the sensitivity and selectivity of the medium. The superior sensitivity and selectivity of the medium were compared with that of normal mCCDA medium without the addition of clavulanic acid in rinsate of chicken. The name of the developed medium is referred to below as C-mCCDA medium (Clavulanic acid B supplemented mCCDA).
본 발명을 통하여 알 수 있는 바와 같이, 본 발명의 배지 조성물은 민감도 및 선택성 면에서 기존의 배지에 비해 뛰어난 효과를 나타낸다.As can be seen from the present invention, the medium composition of the present invention exhibits an excellent effect in sensitivity and selectivity compared with the conventional medium.
도 1은 본 발명의 실험과정 flow chart이다.
도 2는 두 가지 선택배지의 양성검출율 비교(회당 20마리씩 총 6반복, 총 120마리)한 표이다.
도 3은 두 가지 배지의 선택성 비교: 경쟁균이 자란 선택배지의 개수 및 경쟁균의 Growth index 비교(회당 20마리씩 총 6반복, 총 120마리)한 표이다.1 is a flow chart of the experimental procedure of the present invention.
Fig. 2 is a table showing the comparison of the positive detection rates of the two selective mediums (six replicates per 20 rabbits per day, totaling 120 rabbits).
Figure 3 compares the selectivity of the two media: a comparison of the number of selective media in which the competing bacterium grew and the growth index of the competing bacterium (20 replicates per 20 replicates per 120 replicates).
이하, 비한정적인 실시 예를 통하여 본 발명을 더욱 구체적으로 설명한다. 단 하기 실시예는 본 발명을 예시하기 위한 의도로 기재된 것으로서 본 발명의 범위는 하기 실시예에 의하여 제한되는 것으로 해석되지 아니한다.Hereinafter, the present invention will be described more specifically with reference to non-limiting examples. The following examples are intended to illustrate the invention and the scope of the invention is not to be construed as being limited by the following examples.
실시예Example 1:배지의 제조 1: Preparation of medium
기존의 mCCDA 배지는 제조자(Oxoid사, 영국)의 매뉴얼대로 준비되었으며, C- mCCDA 배지는 기존 mCCDA 배지의 성분에 고농도의 clavulanic acid (potassium clavulanate)를 첨가하여 만들었다. 그 조성은 아래의 표에 기재되어 있다. The existing mCCDA medium was prepared according to the manufacturer's manual (Oxoid, UK) and the C-mCCDA medium was prepared by adding high concentration of clavulanic acid (potassium clavulanate) to the components of the mCCDA medium. The composition is shown in the table below.
(OXOID)- mCCDA agar base, 45.5 g
(OXOID)
(OXOID)- mCCDA agar base, 45.5 g
(OXOID)
mCCDA, 2 vials (OXOID)-Antibiotic supplement for
mCCDA, 2 vials (OXOID)
mCCDA, 2 vials (OXOID)
- Clavulanic acid (potassium clavulanate) 0.01-10 mg-Antibiotic supplement for
mCCDA, 2 vials (OXOID)
- Clavulanic acid (potassium clavulanate) 0.01-10 mg
표 3은 본 발명에서 사용된 두 가지 선택배지의 조성을 나타낸 표이다.Table 3 shows the composition of the two selective media used in the present invention.
실시예Example 2: 2: 닭에서의Chicken 개발 배지의 검증실험 Verification experiment of development medium
본 발명에서 개발된 배지를 이용하여 실제 계육샘플에서 그 검출감도를 확인해 보았다. Campylobacter 가 제일 문제가 되는 식품은 닭, 칠면조 등의 가금육으로서, 본 실험에서는 시중에 유통되는 닭을 120마리 사서 mCCDA 배지와 C-mCCDA 배지로 해당 균을 검출하였다. 닭의 린스(rinse) 방법 및 증균방법은 미국 농림부의 식품안전기관인 USDA FSIS의 프로토콜을 바탕으로 하였으며, 아래와 같다.Using the medium developed in the present invention, the detection sensitivity was confirmed in an actual meat sample. In this experiment, 120 chickens were purchased from the market and mCCDA and C-mCCDA media were used for the detection of the bacterium. The method of rinse and the method of propagation of chicken were based on USDA FSIS protocol of US Food and Drug Administration.
계육(전육)을 400ml의 Buffered peptone water과 섞은 후, 1분간 shaking한다. Shaking이 끝난 rinse액중 25ml을 Bolton broth (2X 농도) 25ml과 섞어준 후, 42℃에서 미호기적으로 48시간 배양한다. 배양이 끝난 후, 한 백금이를 mCCDA 배지와 C-mCCDA 배지에 획선 도말한 후, 미호기적 환경에서 42℃에서 48시간 배양한 후, 둥글고 편평한 반투명의 집락을 골라서 성상확인 및 colony PCR을 통하여, Campylobacter에 대한 확인동정을 실시한다. colony PCR은 유전학적으로 Campylobacter의 확인동정을 실시하는 방법으로 방법은 다음과 같다. 계대된 집락을 0.2ml의 멸균증류수에 풀고, 10분간 끓인 후 원심분리하고 상층액을 분리하여 template DNA로 사용한다. DNA는 PCR mix kit인 MaximeTM PCR PreMix (iNtRON biotechnology, Sungnam, Korea)와 섞어주고, forward/reverse primer를 첨가해 준 후, PCR을 돌리고 밴드사이즈를 통해 Campylobacter 양성임을 최종 확인한다. 반응조건, primer의 사용농도, primer의 시퀀스 등은 Denis et al. (Denis et al. Development of a m-PCR assay for simultaneous identification of Campylobacter jejuni and C. coli. Letters in Applied Microbiology 1999, 29, 406-410)에 나온 것과 동일하게 사용하였다. 본 실험의 전반적인 과정은 도 1에 도식화되어 있다. Chicken meat (whole meat) is mixed with 400 ml of Buffered peptone water and shaken for 1 minute. 25 ml of shaking rinse solution is mixed with 25 ml of Bolton broth (2X concentration) and incubated at 42 ° C for 48 hours. After incubation, one platinum aliquot was streaked onto mCCDA medium and C-mCCDA medium. After incubation at 42 ° C for 48 hours in a humid environment, round and flat translucent colonies were selected for identification and colony PCR, Identification of Campylobacter is performed. Colony PCR is a method to identify and identify Campylobacter genetically. Passaged colonies are dissolved in 0.2 ml of sterile distilled water, boiled for 10 minutes, centrifuged and the supernatant is separated and used as template DNA. DNA is mixed with a PCR mix kit, Maxime ™ PCR PreMix (iNtRON biotechnology, Sungnam, Korea), forward / reverse primer is added, PCR is run and final confirmation is confirmed by Campylobacter size. Reaction conditions, primer concentration, and primer sequences are described in Denis et al. (Denis et al., Development of a m-PCR assay for simultaneous identification of Campylobacter jejuni and C. coli . Letters in Applied Microbiology 1999, 29, 406-410). The overall process of this experiment is illustrated in FIG.
실시예Example 3: 비교 및 통계처리 3: Comparison and statistical processing
계육에서의 배지검증 실험의 경우, Chon et al. (Comparison of three selective media and validation of the VIDAS Campylobacter assay for the detection of Campylobacter jejuni in ground beef and fresh-cut vegetables. J Food Prot. 2011. 74:456-60)에 제시된 방법을 이용하여 양성 검출수를 비교함으로서 민감도를 비교 측정하였다. 또한 캠필로박터가 아닌 다른 경쟁균(competing flora)이 발견된 배지의 수를 비교하여 선택성을 비교하였다 (경쟁균이 적다는 것은 불필요한 균을 배제하는 능력이 뛰어난 것을 의미하므로, 경쟁균이 발견된 배지가 적을수록 선택성은 높음). 총 120개의 샘플 중 해당되는 수를 측정하고, GraphPad Instat software (GraphPad Software, Inc. San Diego, CA, USA)를 사용해 Fisher's exact test로 각 배지의 민감도와 선택성을 비교하였다. P value를 측정하여 값이 0.05보다 적으면 유의차가 있는 것으로 판단하였다.In the case of media verification experiments in chickens, Chon et al. (Comparison of three selective media and validation of the VIDAS Campylobacter assay for the detection of Campylobacter jejuni in ground beef and fresh-cut vegetables. J Food Prot. 2011. 74: 456-60). The sensitivity was compared by comparing the number of positive detections. In addition, the selectivity was compared by comparing the number of media in which competing flora other than Campylobacter was found (the less competitive bacteria means superior ability to eliminate unnecessary bacteria, The lower the selectivity is). A total of 120 samples were counted and the sensitivity and selectivity of each medium were compared using Fisher's exact test using GraphPad Instat software (GraphPad Software, Inc. San Diego, Calif., USA). P value was measured and it was judged that there was a significant difference when the value was less than 0.05.
한편 앞선 논문 (Peterz, M. 1991. Comparison of Preston agar and a blood-free selective medium for detection of Campylobacter jejuni in food.. J. Assoc Off Anal Chem. 74:651-654)을 참조하여 각 배지에서 경쟁균이 어느 정도로 성장하는지에 대한 Growth index를 산정하여, 선택성 비교에 참조하였다. Growth index가 높다는 것은 캠필로박터가 아닌 경쟁균이 배지에서 많이 성장한다는 의미로서, growth index가 높을수록 배지의 선택성은 낮아진다. Growth index는 경쟁균이 자란 plate에서만 측정되었고 1-3까지 수치화하였다. 1은 배지에서 소수의 집락만 자라는 것, 2는 배지의 반정도를 덮을 수준의 경쟁균이 자라는 것, 3은 전체 plate를 덮을 수준의 경쟁균이 자라는 것을 의미한다.In the previous paper (Peterz, M. 1991. Comparison of Preston agar and a blood-free selective medium for detection of Campylobacter jejuni in food .. J. Assoc Off Anal Chem . 74: 651-654), the Growth Index was calculated to determine the extent to which the competing strains were grown in each medium, and the growth index was referred to in the selectivity comparison. The higher the growth index, the greater the growth rate of the competitor than the camphor bumper. The higher the growth index, the lower the selectivity of the medium. The growth index was measured only on the plate where the competing strains were grown, and was quantified up to 1-3. 1 means that only a small number of colonies grow in the medium; 2, the competitor grows to cover about half of the medium; and 3, the competent bacteria grows to cover the entire plate.
계육에서의 배지검증 실험을 실험한 결과는 하기에서와 같다.The results of the experiment for the verification of the medium in the broiler meat were as follows.
도 2에서 알 수 있는 바와 같이, C-mCCDA 배지를 사용하였을 때에는 120개 중 67개가 양성으로 검출되고 mCCDA 배지를 사용하였을 경우에는 그 보다 유의적으로 훨씬 적은 38개의 양성이 검출되어 개발된 배지의 민감도가 현재 사용되고 있는 배지에 비해 월등히 좋은 것을 확인하였다. As can be seen from FIG. 2, when the C-mCCDA medium was used, 67 out of 120 cells were detected as positive, and when mCCDA medium was used, 38 positive cells were detected, And the sensitivity was much better than the currently used medium.
또한 도 3에서 알 수 있는 바와 같이, 개발된 C-mCCDA 배지는 120개의 배지중 44개가 다른 경쟁균에 의해 오염되었으나 mCCDA는 그 보다 유의적으로 많은 110개의 배지가 오염되어, C-mCCDA 배지가 훨씬 더 높은 선택성을 보였다. 또한 C-mCCDA의 경쟁균 growth index가 다른 배지에 비해 훨씬 낮아 개발된 배지의 선택성이 더 좋은 것을 확인하였다. As can be seen in FIG. 3, the developed C-mCCDA medium contaminated 44 of the 120 mediums with other competitive bacteria, but mCCDA was significantly more numerous than 110 mediums, and the C-mCCDA medium Showed much higher selectivity. In addition, the competition index of C-mCCDA was much lower than that of the other media, confirming the better selectivity of the medium.
따라서 결론적으로 개발된 C-mCCDA 배지는 민감도와 선택성 면에서 기존의 mCCDA 배지에 비해 훨씬 개선된 성능을 보이는 것으로 보인다.
Therefore, the developed C-mCCDA medium shows much improved performance compared to the conventional mCCDA medium in terms of sensitivity and selectivity.
Claims (8)
상기 조성물의 조성은 뉴트리언트 브로쓰 넘버 2 25 중량부, 세균 활성탄(Bacteriological charcoal) 4 중량부, 카제인 가수분해물(Casein hydrolysate) 3 중량부, 디옥신콜린산 나트륨(Sodium desoxycholate) 1 중량부,황산제일철(Ferrous sulphate) 0.25 중량부, 피루브산 나트륨(Sodium pyruvate) 0.25 중량부 및 아가 12 중량부 비율이고, 포타슘 클라블라네이트는 증류수 1리터에 0.5 mg 함유하고, 세포페라존(Cefoperazone), 및 암포테리신(Amphotericin) B는 1리터에 각각 32 및 10mg포함하는 것을 특징으로 하는 캠필로박터(Campylobacter) jejuni에 대한 민감성을 개선한 배지 조성물.Nutrient broth number 2 of OXOID, Bacteriological charcoal, Casein hydrolyzate, Sodium desoxycholate, Ferrous sulphate, Sodium pyruvate (sodium pyruvate) ), Aga, Cefoperazone, Amphotericin B, and Campylobacter jejuni including potassium clavulanate.
25 parts by weight of Nutrient Broth No. 2, 4 parts by weight of bacteriological charcoal, 3 parts by weight of casein hydrolyzate, 1 part by weight of sodium desoxycholate, 0.25 parts by weight of ferrous sulphate, 0.25 parts by weight of sodium pyruvate and 12 parts by weight of agar. Potassium clavulanate contained 0.5 mg per liter of distilled water, and cellulase (Cefoperazone) and amphotericin Wherein Amphotericin B contains 32 and 10 mg, respectively, per liter. The culture medium according to claim 1, wherein the amphotericin B has a sensitivity to Campylobacter jejuni.
상기 조성물의 조성은 뉴트리언트 브로쓰 넘버 2 25 중량부, 세균 활성탄(Bacteriological charcoal) 4 중량부, 카제인 가수분해물(Casein hydrolysate) 3 중량부, 디옥신콜린산 나트륨(Sodium desoxycholate) 1 중량부,황산제일철(Ferrous sulphate) 0.25 중량부, 피루브산 나트륨(Sodium pyruvate) 0.25 중량부 및 아가 12 중량부 비율이고, 포타슘 클라블라네이트는 증류수 1리터에 0.5 mg 함유하고, 세포페라존(Cefoperazone), 및 암포테리신(Amphotericin) B는 1리터에 각각 32 및 10mg포함하는 것을 특징으로 하는 캠필로박터(Campylobacter) jejuni에 대한 선택성을 개선한 배지 조성물.Nutrient broth number 2 of OXOID, Bacteriological charcoal, Casein hydrolyzate, Sodium desoxycholate, Ferrous sulphate, Sodium pyruvate (sodium pyruvate) ), Agar, Cefoperazone, Amphotericin B, and Campylobacter jejuni including potassium clavulanate.
25 parts by weight of Nutrient Broth No. 2, 4 parts by weight of bacteriological charcoal, 3 parts by weight of casein hydrolyzate, 1 part by weight of sodium desoxycholate, 0.25 parts by weight of ferrous sulphate, 0.25 parts by weight of sodium pyruvate and 12 parts by weight of agar. Potassium clavulanate contained 0.5 mg per liter of distilled water, and cellulase (Cefoperazone) and amphotericin Seen (Amphotericin) B is a medium composition improve selectivity to Campylobacter (Campylobacter), characterized in that, each containing 32 and 10mg in 1 liter jejuni.
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