KR101439105B1

KR101439105B1 - System For Bioconcentration Test

Info

Publication number: KR101439105B1
Application number: KR1020140085048A
Authority: KR
Inventors: 서종수; 이봉재; 김종환; 최성길; 최봉진; 배성열
Original assignee: 계룡과학주식회사; 한국화학연구원
Priority date: 2014-07-08
Filing date: 2014-07-08
Publication date: 2014-09-12

Abstract

The present invention relates to an apparatus for testing bioconcentration. According to the present invention, the apparatus supplies dilution water and an experiment material solution to a mixing tank of a mixing apparatus to dilute the solution to a predetermined ratio, and continuously supplies the mixed liquid to a test tank from the mixing tank so that the apparatus is appropriate for constantly maintaining the concentration inside a specific chemical substance. In addition, the dilution water and the experiment material solution is mixed primarily, secondarily and tertiarily to make a test solution having a constant concentration. Further, discharge water, which has been subject to the test in the test tank, is passed through an activated carbon and drainage provided inside a drainage tank and purified, so environmental pollution can be prevented.

Description

System For Bioconcentration Test "

The present invention relates to a bioaccumulation test apparatus, and more particularly, to a syringe pump operated by control of a controller and diluted water (water) whose flow rate is controlled as a flow meter and a flow rate control valve, It is possible to carry out a bioconcentration test by keeping the amount of dissolved oxygen and temperature required for maintaining the growth of the organisms constant, and to purify and discharge the effluent discharged through the test using activated carbon.

Due to the increase of harmful substances due to industrialization (eg, environmental hormone risk), damage to the ecosystem due to the increase of wastes or pesticides as water pollutants, severity of residual pesticides in food, and heavy pollution of heavy metals in soil The human body and the environment are threatened. It is very important and urgent to monitor the harmfulness caused by chemical substances that adversely affect the human body and the environment. Currently, more than 80,000 kinds of chemical substances are used commercially at home and abroad. Each year, thousands of new substances are used as raw materials for foods, medicines, cosmetics and medical equipment. How much do they affect the human body and the environment? Toxicity And exposure levels and the degree of accumulation of toxic substances in vivo upon exposure are very important.

As a field for securing the safety of such chemical substances, there is an environmental hazard evaluation. To this end, legally required environmental toxicity test items are known, and the above test items are classified into a risk of direct and indirect human and environmental organisms The possibility is tested and analyzed.

All the data related to the exposure dose, the decomposition rate, the concentration rate and the toxicity are analyzed and analyzed through the above-described environmental hazard evaluation, and the results are collected, so that a reasonable evaluation is made for one compound. .

A wide range of environmental monitoring methods are needed to diagnose and respond to such environmental hazards early, and toxicity is tested using recombinant microorganisms, luminescent bacteria or yeast, or using enzymes. They mainly measure harmfulness by optical methods.

In order to protect the ecosystem from the above chemical substances or environmental pollutants and to promote sustainable development, toxicological tests of aquatic organisms (fish, daphnia, etc.) and terrestrial organisms are carried out on industrial chemicals, pesticides, veterinary drugs and pharmaceuticals , And bioconcentration tests on aquatic organisms. All tests are conducted in accordance with the OECD Test Guidelines and test methods in the United States, Japan and Europe. The most important characteristic of the toxicity evaluation and bioconcentration test for aquatic organisms is that they have an indirect route of administration in which the test substance (test solution) is dissolved in water and moved into the organism.

However, the exposure method is an exponential method (Static test) in which the test solution is not exchanged during the test period, a semistatic test in which the test solution is changed in a predetermined period during the test period, a test solution is continuously exchanged (Flow-through test), it is important to select the most appropriate route of exposure for each substance. Such water-soluble formulas are produced manually by hand in order to maintain the concentration of the test substance when the test substance is not stable in the aqueous (water) state, so that the precision and the continuous supply are difficult.

In order to solve such a conventional problem, the prior art document 10-0906180 (the name of the invention: a test solution producing apparatus of the present invention) and the registered patent 10-0958859 (a water-type daphnia toxicity test apparatus) are provided. In this prior art reference 1, a quantitative concentration of diluted water (water) and a stock solution can be secured by using a flow meter and a pump, and diluent water (water) supplied to the first mixing vessel The stock solution is firstly mixed using a stirrer and then dropped into a second mixing vessel to make a test solution to a specific concentration by secondary mixing and then the outlet of the second mixing vessel And it is designed to be able to supply continuously.

The prior art document 2 includes at least one mixing section for mixing a diluting water and a toxic substance with the diluting water supplied from the pumping section to produce a test solution, One or more test specimens are exposed to the test solution

And the test solution is continuously supplied and replaced during the test period so as to be suitable for the ecological characteristics of the water fleas to be tested.

In this prior art document 1, even if the existing chemical substance or newly developed substance is not stable in the aqueous solution state, the test substance can be supplied at a constant concentration using an agitator, and the flow rate of the diluting water can be precisely controlled using a flow meter The test solution can be continuously supplied at a constant concentration by continuously and precisely controlling the supply amount of the test solution per unit time through the pump controlled by the controller and continuously supplying the diluted water and the amount of the test solution, The amount of dissolved oxygen in the solution can be maintained through the continuous supply of oxygen, so that it is not necessary to provide an oxygen supplier separately.

Prior Art 2 can be applied to the water-based exposure method suitable for the ecological characteristics of aquatic organisms, and as it becomes possible to collect accurate test data, it can be expected that the environmental hazard evaluation of harmful substances will be more accurate have.

However, in this prior art document 1, the mixed water mixed by the stirrer in the first mixing vessel is supplied to the second mixing vessel as a solenoid valve operated under the control of the controller, whereby the mixed water is continuously supplied to the second mixing vessel And the dilution ratio of the test solution was kept different in the second mixing vessel due to intermittent discontinuation, and thus an inappropriate problem occurred because the specific chemical substance could not be exposed to the organism continuously and at a constant concentration.

In addition, the prior art 2 requires a large number of pumps and chambers, which are composed of a pumping section, a mixing section, a branching section and an exposing section, so that the apparatus is complicated and a lot of space and cost are required for installation. In the upper chamber of the mixing section, It is not easy to prepare a test solution of a certain concentration due to the mixing of the diluted water and the toxic substances as the rotational force of the upper chamber and the intermediate chamber and the supply is not easy due to the discharge through the discharge hose connected to one side between the upper chamber and the intermediate chamber A problem has occurred.

Disclosure of the Invention The present invention has been made in order to solve the conventional problems of a bioreactor test which is more versatile than a pouring device capable of confirming half size of fish (i.e., half of fish die) The diluted mixed water is fed into the mixed water tank and diluted to a predetermined ratio, and the diluted mixed water is continuously supplied to the test water tank.

Another object of the present invention is to mix diluted water and a solution of a test substance in the first, second and third stages to supply a test solution of a certain concentration to the test water tank so that the organism in the test water tank is properly exposed to a certain concentration of the test solution have.

Another object of the present invention is to purify the discharged water discharged through the test in the test water tank by a supplied amount through a drain water purifier provided in the drain water tank and discharge the purified water to the outside.

According to an aspect of the present invention, there is provided a washing machine comprising: a diluting water storage tank for storing diluted water; A test solution container for storing a test solution; A flow meter and a flow rate control valve for discharging diluted water of the dilution water storage tank by a predetermined amount; A mixing and discharging device for supplying diluted water and test solution from the diluting water storage tank and the test solution container, A stirring device for stirring the diluted water and the test solution in the mixing and discharging device with the mixed water to maintain the test solution at a specific concentration; A syringe pump for feeding a test solution of the test solution container into the mixing and discharging device in a predetermined amount; A controller for controlling the syringe pump to supply the test solution at the set concentration calculated by the test solution supply amount per unit time; It is composed of a test water tank provided at the lower part of the mixing and discharging unit and used to test the dissolved oxygen amount as aquatic organisms by receiving the diluted mixed water from the mixing and discharging unit. Wherein the mixing and discharging device comprises: a lower pedestal having a circular seating groove formed at an upper portion thereof; A mixing water tank for mixing and diluting the diluted water (water) with the test solution by means of an agitating device, the mixed water being introduced into the circular recessed groove of the lower pedestal so as to prevent water leakage; A discharge port formed on both sides of the upper portion of the mixing water tank to communicate with each other at the same height so as to discharge mixed water to the outside; An overflow hose whose upper end is connected to the outlet and whose lower end is connected to a "Y" And a supply hose connected to an upper portion of the lower portion of the "Y" -shaped connector to supply mixed water to the test water tank, and is mounted on the vertical moving plate of the front and rear moving devices.

According to the present invention, the diluted water and the experimental material solution, which are maintained at a saturation concentration of 60% and a temperature of 20 to 25 ° C and maintained at a temperature within 2 ° C, are fed into the mixing water tank of the mixing apparatus, And the diluted mixed water is continuously supplied to the test water tank from the mixed water tank. By supplying the diluted mixed water to the test water tank while maintaining the set dilution ratio, it is suitable to keep the concentration of the specific chemical in the living water constant In addition, it was possible to prepare a test solution with a constant concentration by mixing the diluted water and the test substance solution in the first, second, and third levels. Also, the effluent water generated in the test water tank was supplied to the activated carbon, It is possible to prevent the environmental pollution by discharging the purified water through the cylinder.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a schematic view showing a bioaccumulation test apparatus according to the present invention; FIG.
2 is a perspective view of a bioaccumulation test apparatus according to the present invention.
FIG. 3 is a front view of a bioaccumulation test apparatus according to the present invention. FIG.
4 is an enlarged view of the portion "A" in Fig.
FIG. 5 is a side view of a bioconcentration test apparatus according to the present invention. FIG.
Fig. 6 is a front view of the main part of the bioaccumulation test apparatus according to the present invention. Fig.
Fig. 7 is an enlarged configuration view of the portion "B" in Fig. 6; Fig.
8 is a configuration diagram showing a mixing apparatus of a bioaccumulation test apparatus according to the present invention.
Fig. 9 is an enlarged configuration view for the portion "C" in Fig. 8; Fig.

Hereinafter, the present invention will be described in detail with reference to the accompanying drawings.

FIG. 2 is a perspective view of a bioaccumulation test apparatus according to the present invention. FIG. 3 is a schematic view showing a bioconcentration test apparatus according to the present invention, FIG. 4 is an enlarged configuration view of the "A" portion of FIG. 3, and FIG. 5 is a side structural view of the bioaccumulation test apparatus according to the present invention.

1 to 5, the bioaccumulation test apparatus according to the present invention includes an upper horizontal frame 2, an upper horizontal frame 3, a lower horizontal frame 4, a lower horizontal frame 5, and a vertical A worktable (1) assembled and held as a frame (6) and finished with a vertical finishing panel (7); A guide bar 12 passing through the vertical finishing panel 7 of the workbench 1 so as to be fixed between the front and rear of the upper horizontal frame 2 and the upper horizontal frame 3, A front and rear moving device (10) comprising a vertical moving plate (14) movably coupled to the moving plate (14); A dilution water storage tank 20 mounted above the upper horizontal frame 2 for storing dilution water (water); A test solution container (30) placed in the lower horizontal frame (4) for storing the test solution; A flow meter 40 and a flow control valve 41 mounted on the vertical finishing panel 7 and connected to the dilution water drain pipe 20a connected to the lower portion of the dilution water storage tank 20 to discharge diluted water in a predetermined amount; A predetermined amount of dilution water is supplied from the dilution water storage tank 20 and a predetermined amount of the test solution is supplied from the test solution container 30 through the vertical collection tube 60 to mix the diluted water and the test solution, A mixing and discharging device (50) mounted on the vertical movable plate (14) of the front and rear moving device (10) for discharging to the bottom; A stirrer 70 for vertically mounting the vertical moving plate 14 on the mixing and discharging device 50 to dilute water supplied into the mixing device 50 and stirring the test solution with the mixed water to maintain the concentration thereof at a specific concentration )Wow; A syringe pump 80 for supplying a predetermined amount of the test solution stored in the test solution container 30 to the mixing device 50; A controller (90) for controlling the syringe pump (80) so as to supply the test solution at a predetermined concentration calculated as the test solution supply amount per unit time; The diluted mixed water supplied from the mixing and discharging device 50 is supplied to the test water tank (not shown) mounted on the lower horizontal frame 4 to test the dissolved oxygen amount included in the mixed water with aquatic organisms (carp, carp, 100); A siphon (110) for discharging the drain water, which is contained in the test water tank (100) and has been tested, while maintaining the water level in the drain water tank (120) provided in the lower region; And a drain water purifier 130 provided in the drain water tank 120 to purify the drain water and discharge the drain water to the outside.

The dilution water storage tank 20 is mounted on the upper end of the upper horizontal frame 2 and is equipped with a dilution water supply device 21 for supplying dilution water and is provided with a water level sensor 22 for maintaining the water level, A heater 23 is provided to heat the dilution water. A temperature sensor 24 is provided to maintain the temperature of the heated water. An oxygen supply device 25 is provided to supply oxygen to the inside of the dilution water, So that the saturated concentration of the oxygen amount was 60%.

At this time, the diluting water supply device 21 includes a main valve 21a connected to the water line, a filter 21b for filtering the foreign substances contained in the supplied water, and a water supply solenoid valve 21c, The filtered dilution water (water) is supplied.

The mixing and discharging device 50 includes a lower pedestal 51 which is detachably mounted on the vertical movable plate 14 of the front and rear moving device 10 and has a circular seating groove 51a formed thereon, Wow; The lower end of the lower mount 51 is seated and fixed in the circular seating groove 51a so as to prevent water leakage and the mixed water is introduced into the mixing water tank 52 for diluting the diluted water and the test solution by the stirring device 70 )Wow; Discharge ports 53 and 54 which are formed on both sides of the upper portion of the mixing water tank 52 so as to communicate with each other at the same height so as to discharge the mixed water to the outside; An overflow hose 56 whose upper end is connected to the outlet 53 (54) and whose lower end is connected to the "Y" And a supply hose 57 connected to an upper portion of the lower portion of the "Y" -shaped connector 55 to supply mixed water to the test water tank 100. At this time, the water level of the mixed water in the mixing water tank 52 is uniformly positioned between the discharge ports 53 and 54 on the upper part of the mixing water tank 52 as shown in FIG.

A vertical through hole 58 is formed in the circular mounting recess 51a of the lower pedestal 51 and an auxiliary overflow hose 59 is connected between the vertical through hole 58 and the "Y" , And the mixed water overflowing the mixing water tank (52) is safely discharged by the stirring device (70).

At this time, the dilution water of the dilution water storage tank 20 whose flow rate is controlled by the flow meter 40 and the flow rate control valve 41 is calculated by the syringe pump 80 controlled by the controller 90 and the test solution supply amount per unit time The test solution in the test solution container 30 supplied at a predetermined concentration is flowed at about 200 ml / min and 10 to 100 ul / min to maintain the concentration of the test water tank 100, And is supplied into the mixing water tank 52 of the mixing and discharging device 50 and diluted to about 2,000 to 20,000 times.

The vertical collection pipe (60) includes a bracket (62) fixed to the vertical movable plate (14); A vertical tube 64 vertically fixed to the bracket 62 and having a lower end located close to the bottom of the mixing water tank 52; And a collecting part 66 integrally formed on the upper end of the vertical tube 64 and connected to the dilution water discharge pipe 20a and to which the test solution supply pipe 32 is inserted.

At this time, the test solution supply pipe 32 is inserted into the collecting part 66, and the lower end is positioned below the vertical tube 64, whereby the test solution and the dilution water are mixed first.

The stirring device 70 includes a motor 72 mounted on the vertical movable plate 14 above the mixing device 50; An agitating shaft 74 rotatably mounted on the motor 72 so as to be vertically positioned at the center inside the mixing water tank 52 of the mixing and discharging device 50; And a blade 76 coupled to the lower end of the agitating shaft 74. The test water is supplied to the test water tank 100 by continuously mixing the diluted water and the test solution in the second mix and continuously maintaining the specific concentration required for the test.

The pump 80 controls the supply of the test solution per unit time (10 to 100 ul) per unit time under the control of the controller 90 in order to supply the test solution stored in the test solution container 30 to the mixing / / min) and supplied into the mixing water tank 52 of the mixing and discharging device 50 at a predetermined concentration.

The test water tank 100 is placed on the drain water tank 120 and is supplied with the diluted mixed water supplied through the mixing and discharging device 50 and supplied to the aquatic organisms ), And the test substance is exposed to the test substance to determine whether the test substance is concentrated in the living body.

The siphon 110 is formed with a latching portion 111 to be hooked on the upper end of the test water tank 100. The lower end of the siphon 110 is located near the bottom surface of the test water tank 120, A bend section (112) located on the outer surface of the water tub (120); And a discharge water discharge hole 113 and an air inflow hole 114 through which the discharge water is discharged and into which the air is introduced.

1 and 5, a line is formed at a position near the drainage hole 114 of the siphon 110 and is maintained at a constant level.

The drain water purifier 130 includes a drain trap 132 disposed in a central region of the drain water tank 120 and discharging drain water downward; A drainage network 134 detachably coupled within the drainage trap 132; A drainage cylinder 136 which is seated on the drainage wire network 134 and has a plurality of vertical holes 136a formed on an outer circumferential surface thereof and which surrounds the vertical holes 136a and to which the net 136b is fixed; And an activated carbon 138 which is filled in the drainage water tank 120 and inside the drainage cylinder 136 to filter out foreign substances contained in the drain water while passing the drain water. At this time, the activated carbon 138 is made of granules that do not pass through the wire mesh formed in the drainage wire network 134 and the net 136b.

The test solution container 30, the mixing water tank 52, the "Y" -shaped connection pipe 55, the vertical collection pipe 60, the test water tank 100 and the siphon 110 are made of tempered glass and the test solution supply hose 32, the overflow hose 56, the supply hose 58 and the auxiliary overflow hose 59 are made of Teflon and the stirring shaft 74, (76) and drainage tank (120) are coated with Teflon to prevent chemical reaction or adsorption to the test solution.

The operation state of the bioaccumulation test apparatus according to the present invention thus configured will be briefly described below.

1 to 9, the dilution water (water) stored in the dilution water storage tank 20 mounted on the upper end of the upper horizontal frame 2 of the work table 1 is supplied to the dilution water supply device 22, The water level is maintained by the heater 23 and the temperature sensor 24 and the oxygen is supplied by the oxygen supply device 25 to be dissolved The saturated concentration of the oxygen amount is maintained at 60% and is adjusted to a predetermined amount by the flow meter 40 and the flow control valve 41 to be supplied to the vertical collection pipe 60 along the dilution water discharge pipe 20a under the dilution water storage tank 20. [ Is supplied to the collecting section 66 of the apparatus.

At the same time, the test solution in the test solution container 30 is maintained at the concentration determined by calculating the test solution supply amount per unit time by the operation of the syringe pump 80 controlled by the controller 90, The dilution water and the test solution are firstly mixed in the vertical tube 64 of the vertical collection tube 60 as supplied to the collection section 66 of the vertical collection tube 60 9)

The mixed water primarily mixed as described above is diluted to about 2,000 to 20,000 times into the mixed water tank 52 constituting the mixing and discharging device 50 through the vertical pipe 64 of the vertical collecting pipe 60, As the stirring shaft 74 is rotated by the operation of the motor 72 constituting the apparatus 70, the blade 76 at the lower end of the stirring shaft 74 rotates and the diluted water and the test solution (See Fig. 8). &Lt; tb > < TABLE >

The mixed water is stirred in the mixing water tank 52 by the continuous rotation of the blades 76 and then discharged through the discharge ports 53 and 54 formed at the same height on both sides of the mixing water tank 52, The upper end is connected to the discharge ports 53 and 54 and is discharged to the overflow hose 56 connected to the lower end of the "Y" 55 and is continuously supplied to the test water tank 100 through the supply hose 56 connected to the lower part.

As a result, aquatic organisms (carp, carp, etc.) grow on the dissolved oxygen amount contained in the mixed water supplied into the test water tank 100, and the test substance is exposed to the test substance to determine whether the test substance is concentrated in the living body do.

3 and 4, the drain water supplied from the test water tank 100 and discharged through the test is discharged to the bent portion 112 of the siphon 110, which is hooked on the upper end of the test water tank 100, The water is discharged to the outside through the drain water discharge hole 113 and the drain water is supplied to the drain water tank 120 through the drain water purifier 130 provided inside.

That is, the activated carbon 138 filled in the drainage tank 120 and the net 136b of the drainage cylinder 136 seated on the drainage wire network 134 detachably coupled to the inside of the drainage trap 132 and the vertical holes 136a, purified through a drainage network 134 and a drain trap 132, discharged to the outside, and treated.

As described above, the diluted water and the test solution are diluted to a predetermined concentration and continuously supplied to the test water tank 100 to expose the test solution to the sea lion, carp, etc. and determine whether the test solution is concentrated in the living body. The purified water is continuously discharged through the drain water purifier 130 to the outside.

5, the mixing and discharging device 50, the vertical collection pipe 60 and the stirring device 70 are mounted on the vertical moving plate 14 constituting the front and rear moving device 10, It is possible to selectively adjust the position with respect to the test water tub 100 so as to facilitate the supply of the mixed water.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments. .

1: Workbench 2: Upper horizontal frame
3: upper horizontal frame 10: front and rear moving device
12: Guide rod 14: Vertical copper plate
20: Dilution water storage tank 30: Test solution container
40: Flow meter 41: Flow control valve
50: Mixing and discharging device 51: Lower pedestal
51a: circular seating groove 52: mixing tank
53,54: Outlet 55: "Y"
56: overflow hose 57: supply hose
58: Vertical through hole 59: Auxiliary overflow hose
60: vertical collection tube 62: bracket
64: vertical tube 66:
100: Test water tank 110: Siphon
111: engaging portion 112: bent portion
113: drain water discharge hole 114: air inlet hole
120: drain water tank 130: drain water purifier
132: drain trap 134: drainage wire mesh
136: drainage cylinder 136a: vertical passage
136b: net 138: Activated carbon

Claims

A worktable 1 to be assembled with an upper horizontal frame 2, an upper horizontal frame 3, a lower horizontal frame 4, a lower horizontal frame 5 and a vertical frame 6; A diluting water storage tank 20 mounted on the upper horizontal frame 2 for storing dilution water (water); A test solution container 30 for storing a test solution; A flow meter 40 and a flow control valve 41 for discharging diluted water of the dilution water storage tank 20 by a predetermined amount; A mixing and discharging device (50) for supplying diluted water and a test solution from the diluting water storage tank (20) and the test solution container (30), mixing and diluting the diluted water and the test solution; A stirring device 70 for stirring the diluted water and the test solution in the mixing and discharging device 50 with the mixed water to keep the test solution at a specific concentration; A syringe pump 80 for supplying the test solution of the test solution container 30 to the mixing and discharging device 50; A controller (90) for controlling the syringe pump (80) so as to supply the test solution at a predetermined concentration calculated as the test solution supply amount per unit time; And a test water tank (100) provided below the mixing and discharging device (50) to receive mixed water diluted in the mixing and discharging device (50) and to test the dissolved oxygen amount with aquatic organisms. And continuously supplying the test water to the test water tank 100,
The mixing and discharging device (50)
A lower pedestal 51 having a circular seating groove 51a at an upper portion thereof;
The lower end of the lower pedestal 51 is seated and fixed in the circular seating groove 51a so as to prevent leakage of the mixed water. The mixing water is introduced into the circular receiving recess 51a of the lower pedestal 51 to mix and dilute the diluted water (water) (52);
An outlet 53 (54) formed outwardly on both sides of the mixing water tank (52) so as to communicate with each other at the same height and discharging mixed water to the outside;
An overflow hose 56 whose upper end is connected to the discharge ports 53 and 54 and whose lower end is connected to the "Y"
And a supply hose (57) connected to an upper portion of the lower portion of the "Y " -shaped connector (55) to supply mixed water to the test water tank (100).

2. The apparatus according to claim 1, wherein a vertical through hole (58) is formed in the circular seating groove (51a) of the lower pedestal (51)
Wherein an auxiliary overflow hose (59) is connected between the vertical through hole (58) and the "Y" shaped connecting port (55) to securely discharge mixed water over the mixing water tank (52) Test equipment.

The method according to claim 1,
A guide bar 12 fixed correspondingly between front and rear sides of the upper horizontal frame 2 and the upper horizontal frame 3 of the work table 1,
A vertical movable plate 14 of a front and rear moving device 10 constituted by vertical movable plates 14 movably coupled to upper and lower portions of the guide bars 12 forward and backward,
Wherein the mixing and discharging device (50) and the agitating device (70) are installed to move forward and backward to adjust the position of the mixing and discharging device (50) and the stirring water tank (100).

The method of claim 3,
A bracket 62 fixed to the vertical movement plate 14;
A vertical tube 64 vertically fixed to the bracket 62 and having a lower end positioned close to the bottom of the mixing water tank 52 and a dilution water pipe 20a formed at the upper end of the vertical tube 64, Wherein the diluting water and the test solution are mixed with each other and supplied to the mixing water tank (52) through a vertical collecting pipe (60) composed of a collecting part (66) into which the test solution supplying pipe (32) is inserted.

The test apparatus according to claim 1, wherein a latching portion (111) is formed to be hooked on an upper end of the test water tank (100), a lower end of the test water tank (120) A bending portion 112 located on the outer surface of the test water tank 120;
The water level of the water in the test water tank 100 through the siphon 110 which is formed integrally with the upper portion of the bending portion 112 and is formed integrally with the water outlet hole 113 and the air inflow hole 114, So that the biocompatible material is discharged.

The apparatus according to claim 1, further comprising a drain water purifier (130) in the drain water tank (120) for purifying the drain water generated in the test water tank (100)
The exhaust purification water system (130)
A drain trap (132) provided in a central region of the drain water tank (120) and discharging drain water downward;
A drainage network 134 detachably coupled within the drainage trap 132;
A drainage cylinder 136 which is seated on the drainage wire network 134 and has a plurality of vertical holes 136a formed on an outer circumferential surface thereof and has a netting 136b surrounding the vertical holes 136a;
And an activated carbon (138) filled in the drainage water tank (120) and inside the drainage pipe (136) to filter out foreign substances contained in the drain water while passing the drain water.