KR101438767B1 - High density micro-droplet forming and lodging system and method - Google Patents

High density micro-droplet forming and lodging system and method Download PDF

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Publication number
KR101438767B1
KR101438767B1 KR1020140086062A KR20140086062A KR101438767B1 KR 101438767 B1 KR101438767 B1 KR 101438767B1 KR 1020140086062 A KR1020140086062 A KR 1020140086062A KR 20140086062 A KR20140086062 A KR 20140086062A KR 101438767 B1 KR101438767 B1 KR 101438767B1
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South Korea
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liquid
porous template
microwell
space
upper plate
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KR1020140086062A
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Korean (ko)
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송시몬
김지태
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한양대학교 산학협력단
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B81—MICROSTRUCTURAL TECHNOLOGY
    • B81B—MICROSTRUCTURAL DEVICES OR SYSTEMS, e.g. MICROMECHANICAL DEVICES
    • B81B1/00—Devices without movable or flexible elements, e.g. microcapillary devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices

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  • Life Sciences & Earth Sciences (AREA)
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Abstract

The present invention comprises a porous template with a space part filled with a first liquid and a plurality of micro-wells connected to the space part to be able to communicate; a vacuum absorption part which is disposed on the lower part of the porous template and supplies the first liquid filled in the space part to the micro-well; and a cover part which is disposed on the upper part of the porous template and has an injection hole through which a second liquid flows, wherein the second liquid is not mixed with the first liquid. The surface tension of the first liquid is greater than the surface tension of the second liquid.

Description

TECHNICAL FIELD [0001] The present invention relates to a high density micro-droplet forming array system and method,

The present invention relates to a high density microdroplet production array system and method, and more particularly, to a high density microdroplet generation array system capable of generating microdroplets in a porous template by using interfacial tension between two liquids, System and method.

Currently, research and development of ultra-small analyzing devices for analyzing a large number of samples and reagents in accordance with the miniaturization of analytical technology are actively being carried out, and a plurality of channels and microstructures are included in one small chip, Technologies such as biochips, lab-on-a-chip and micro total analysis systems, which require complex fluid control techniques to perform all the necessary processes, .

Recently, micro droplet generation and control technology using a droplet-based microfluidic chip has been actively studied.

In the past, liquid droplets were generated using a microfluidic chip having flow-focusing or T-juction structure, and droplets were classified or rearranged.

However, in a situation where there is a flow of liquid in a channel formed on a microfluidic chip, the process of generating droplets and regularly rearranging them requires a high-level droplet control technique. In addition, there is considerable difficulty in integrating these microfluidic devices into the microfluidic chip.

On the other hand, the micromolding technique using a template does not require a flow rate control and a droplet control technique, has an advantage of being easy to integrate with other micro devices, and has a real- time monitoring, multi-probe sensors, and digital counting assays.

However, the micromolding technique using a conventional template has a disadvantage in that it is difficult to generate a droplet in a high-density arrangement. Further, in order to generate a sphere-type droplet, a Laplace pressure a structure having a different curvature must be formed on the template.

When a high density droplet formation and a droplet formation are simultaneously performed by a micro molding technique using a conventional template, a separate technique for generation and arrangement of the droplet should be applied.

Accordingly, the present applicant has developed the present invention in order to solve the above-mentioned problems, and as a related art document, Korean Patent Laid-Open Publication No. 10-2013-010471, 'Method of manufacturing microspheres using a replica mold' have.

SUMMARY OF THE INVENTION The present invention provides a high density micro droplet generation array system and method capable of generating high density droplets on a microwell and implementing droplet alignment or fixation simultaneously with generation of droplets do.

The present invention provides a high density microdroplet generating array system and method by which the contact line between the liquid and the template can proceed in both the vertical and horizontal directions by utilizing the difference in interfacial tension between the two liquids used for droplet generation.

The present invention provides a porous template having a space portion filled with a first liquid and a plurality of microwells connected to the space portion so as to communicate with each other; A vacuum suction unit provided below the porous template for allowing a first liquid filled in the space to flow into the microwell; And a cover part provided on the porous template and formed with an injection hole into which a second liquid not mixed with the first liquid flows, wherein the surface tension of the first liquid is higher than the surface tension of the second liquid It can include large ones.

In addition, the second liquid may include one having a greater wettability with respect to the porous template than the first liquid.

Also, the vacuum suction unit may include: an upper plate on which the porous template is placed, on which a plurality of first air flow paths are formed at positions corresponding to the porous template; A lower plate provided below the upper plate; A connection member which is disposed between the upper plate and the lower plate and connects the upper plate and the lower plate and has a second air flow path communicably connected to the plurality of first air flow paths; And a vacuum pump connected to the suction hole formed in any one of the upper plate and the lower plate so as to communicate with the second air passage.

The second air passage may include a first opening forming a region corresponding to a region formed by the plurality of first air passages; And a second opening portion having one end connected to the first opening portion and the other end extending in a direction in which the suction hole is formed and communicably connected to the suction hole.

The connection member may include an adhesive material coated on both sides thereof.

In addition, the droplet of the first liquid may be fixedly arranged in the microwell, and the droplet may be fixedly arranged in the microwell in a spherical or dome-like shape.

The porous template may move the contact line between the first liquid and the second liquid in a vertical direction along the sidewall surface of the microwell and then horizontally along the bottom surface of the microwell.

A laminating step of laminating the porous template on an upper plate of the vacuum suction part; A first liquid injection step of filling the space portion of the porous template stacked in the stacking step with the first liquid; Arranging and fixing the first liquid filled in the space part in the first liquid injecting step into the microwell of the porous template; And a second liquid injecting step of injecting a second liquid into the space portion of the porous template.

In the arranging and fixing step, the vacuum pump is driven to suck and remove the air present in the plurality of microwells, and the first liquid filled in the space is introduced into the space formed by the microwells .

In addition, in the arranging and fixing step, the first liquid may be removed from the space portion when excess first liquid remaining in the plurality of microwells is left in the space portion.

Also, air may be present in the lower edge or corner of the microwell filled with the first liquid.

In addition, in the second liquid injecting step, a second liquid having a surface tension smaller than the surface tension of the first liquid is injected into the space portion without being mixed with the first liquid, 1 < / RTI > liquid droplets.

In addition, when the second liquid flows into the space, a difference in interfacial tension occurs between the first liquid and the second liquid, and a contact line between the first liquid and the second liquid is formed on the vertical wall surface And moving along a horizontal plane of the microwell via a lower edge or a corner.

The high density microdroplet generating array system and method of the present invention can simultaneously generate droplets and simultaneously arrange and fix using the interfacial tension between two liquids.

In addition, the system and method for producing high density micro droplets of the present invention is capable of generating spherical or hemispherical (or dome-shaped) droplets in a microwell having a symmetrical structure and reducing the interval between a plurality of microwells, Arrangement is possible.

Further, the high density microdroplet generating array system and method of the present invention can simply generate and arrange droplets without using an external device for flow rate control and droplet control.

In addition, the high density microdroplet generating array system and method of the present invention can generate liquid droplets in a uniform size, and can control the size of droplets by controlling the length and height of microwells.

1 is an exploded perspective view of a high density microdroplet generating array system in accordance with an embodiment of the present invention.
2 is an exploded perspective view of a vacuum suction unit according to an embodiment of the present invention;
3 is a top plan view of a porous template according to an embodiment of the present invention.
4 is a diagram illustrating a process of generating a droplet by a high-density micro-droplet system according to an embodiment of the present invention.
5 is a diagram illustrating a process of generating a spherical droplet in a microwell according to an embodiment of the present invention.
6 is a flowchart of a high density microdroplet generating array method according to an embodiment of the present invention.
7 is a graph showing the percentage of droplets generated in accordance with an aspect ratio of a microwell in accordance with an embodiment of the present invention.

BRIEF DESCRIPTION OF THE DRAWINGS The advantages and features of the present invention, and the manner of achieving them, will be apparent from and elucidated with reference to the embodiments described hereinafter in conjunction with the accompanying drawings.

It should be understood, however, that the invention is not limited to the disclosed embodiments, but is capable of many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, To fully disclose the scope of the invention to those skilled in the art, and the invention is only defined by the scope of the claims.

Hereinafter, a high density microdroplet generating array system and method according to an embodiment of the present invention will be described in detail with reference to FIGS. 1 to 6. FIG. In describing the present invention, the detailed description of known functions and configurations incorporated herein will be omitted so as not to obscure the gist of the invention.

1, a high density microdroplet generating array system 100 according to an embodiment of the present invention includes a space portion 111 in which a first liquid is filled, A porous template 110 having a plurality of microwells 112 formed therein; A vacuum suction unit 120 for sucking air existing in the microwell 112 of the porous template 110 to allow the first liquid filled in the space 111 to flow into the microwell 112; And a cover 130 formed on the porous template 110 and having an injection hole 131 through which a second liquid not mixed with the first liquid is injected.

The porous template 110 may be made of PDMS (polydimethylsiloxane) material, and may be made of a hydrophobic or hydrophilic material depending on the type of the first liquid or the second liquid. However, any material may be used as long as the porous template 110 has porosity. In addition, if the porous template 110 is formed of a porous material, the surface of the template may be hydrophobic or hydrophilic, or may be used independently.

The porous template 110 has a space 111 formed at a predetermined depth from the top surface of the porous template 110 or formed at an obtuse angle with the porous template 110, A space can be accommodated.

A plurality of microwelles 112 formed in the porous template 110 form a space for arranging and fixing liquid droplets of the first liquid filled in the space portion 111. [ As shown in FIG. 3, a plurality of grooves having a hexagonal shape may be formed on the bottom surface of the porous template 111 partitioning the space 111 at regular intervals.

For example, in the embodiment of the present invention, the microwell 112 is formed of a honeycomb having a hexagonal shape so that the droplet of the first liquid filled in the space 111 can be generated as much as possible within a predetermined area. ) Structure. However, the present invention is not limited thereto, and may be formed in various shapes such as a square, a triangle, and a circle. That is, it is necessary to be able to generate as many droplets as possible by using the space portion 111 having a limited area or size so that high density micro droplets can be generated and arranged. To this end, the microwells 112 Lt; / RTI > Accordingly, the shape of the microwell 112 is not limited to the honeycomb shape as long as the microwell 112 can be formed as much as possible.

As described above, the porous template 110 may be made of a material having hydrophobic or hydrophilic properties depending on the type of the first liquid or the second liquid. For example, if the first liquid is water, the porous template 110 is preferably made of a hydrophobic material. If the first liquid is oil, the porous template 110 is preferably made of a hydrophilic material. However, as described above, if the template 110 is formed of a porous material, the surface of the template 110 may be used to generate droplets irrespective of whether the surface is hydrophobic or hydrophilic.

At this time, it is preferable that the second liquid is selected as a liquid having a higher wettability with respect to the porous template 110 than the first liquid, and is also selected as a liquid having a surface tension smaller than the surface tension of the first liquid .

The vacuum suction unit 120 is disposed under the porous template 110 as shown in FIG. 1, and provides a space in which the porous template 110 can be placed.

The vacuum suction unit 120 sucks the porous template 110 in contact with the bottom surface of the porous template 110 to fill the space 111 of the porous template 110, The first liquid is allowed to flow into the plurality of microwells 112 and the air formed in the microwell 112 is sucked in the course of filling the space 111 with the first liquid do.

2 and 4, the vacuum suction unit 120 includes an upper plate 121 on which the porous template 110 is placed and on which a plurality of first air flow paths 122 are formed; A lower plate 123 provided under the upper plate 121; A plurality of first air flow paths 122 communicating with the plurality of first air flow paths 122 and disposed between the upper plate 121 and the lower plate 123 to connect the upper plate 121 and the lower plate 123, A connecting member 125 formed with two air flow paths 124; And a vacuum pump connected to the suction hole 127 formed in any one of the upper plate 121 and the lower plate 123 so as to communicate with the second air passage 124 formed in the connecting member 125. [ 129).

As described above, the vacuum suction unit 120 according to the present invention can be formed as a structure having three layers.

The first air flow path 122 formed in the upper plate 121 is formed along the thickness direction of the upper plate 121 and is formed in a plurality of spaces spaced apart from each other to define an area formed by the porous template 110 May be formed on the upper plate 121 so as to have an area corresponding to that of the upper plate 121.

It is preferable that the entire area where the first air flow path 122 is formed is not smaller than the entire area of the porous template 110 where the microwell 112 is formed.

The lower plate 123 is formed in a shape corresponding to the upper plate 121 and may be connected to the upper plate 121 via the connecting member 125 as described above.

As shown in FIG. 2, the second air passage 124 formed in the connecting member 125 includes a first opening 124a communicably connected to the plurality of first air passage 122, The first opening 124a may have one end connected to the suction hole 127 and the other end may extend in the direction in which the suction hole 127 is disposed and may include a second opening 124b communicably connected to the suction hole 127 have.

The second air passage 124 including the first opening 124a and the second opening 124b may be formed in such a manner that the connecting member 125 is connected to the upper plate 121, The upper surface of the upper plate 121 and the upper surface of the lower plate 123 and the inner surface of the connecting member 125 when the upper plate 121 and the lower plate 123 are coupled to each other.

The first opening 124a may be formed to have a size corresponding to a region of the first air passage 121a formed on the upper plate 121. [

The second opening 124b may guide the air introduced through the first air passage 122 and the first opening 124a to the suction hole 127. [

The connecting member 125 may be coated with an adhesive material on both sides in order to join the upper plate 121 and the lower plate 123. The upper plate 121 and the lower plate 123 may be made of plastic such as acryl and the microwell 112 may be formed of a CO 2 laser. The connection member 125 is preferably formed of a pressure sensitive adhesive tape.

The suction hole 127 may be formed in either the upper plate 121 or the lower plate 123 and may be connected to the vacuum pump 129 as described above. For reference, the suction hole 127 is described as being formed in the upper plate 121 in the embodiment of the present invention.

The vacuum pump 129 can suck air existing in the microwell 112 through the suction hole 127. That is, when the vacuum pump 129 is driven, the air existing in the microwell 112 passes through the first air passage 122 and the second air passage 124, To be discharged to the outside of the vacuum suction unit (120). Air trapped in the microwell 112 can be discharged through the template 110 because the template 110 has porosity.

For reference, the vacuum pump 129 is a vacuum inhaler widely used in general industrial fields, and can be easily used by anyone skilled in the art, so that detailed description of the construction of the vacuum pump 129 is omitted in the specification of the present invention.

As shown in FIG. 1, the cover 130 has a shape and a size to cover the upper portion of the space 111 of the porous plate 110, and can be made of a plastic material.

The cover 130 may be formed with an injection hole 131 through which the second liquid flows into the space 111 of the porous template 110, as described above.

The cover 130 is filled with the second liquid filled in the space 111 through the injection hole 131 and filled in the space formed by the space 111, A discharge hole 132 may be formed to allow the discharge gas to be discharged from the discharge port 111.

In addition, the shape or position of the injection hole 131 and the discharge hole 132 may be changed so that the second liquid can completely fill the interior of the space portion 111.

Hereinafter, a high-density micro-droplet generation array method by a high-density micro-droplet generation array system according to an embodiment of the present invention will be described.

The method of arranging high density microdroplets according to an embodiment of the present invention includes stacking step S10 of stacking the porous template 110 on the upper plate 121 of the vacuum suction unit 120 as shown in FIG. ); A first liquid injection step (S20) of filling a space portion (111) of the porous template (110) stacked in the laminating step (S10) with a first liquid; A step S30 of arranging and fixing the first liquid filled in the space 111 in the first liquid injection step S20 into the microwell 112 of the porous template 110; And a second liquid injection step (S40) of injecting a second liquid into the space part (111) of the porous template (110).

4 (a), the porous template 110 is placed on top of a plurality of first air channels 122 formed in the upper plate 121. In the laminating step (S10), as shown in FIG. Here, it is preferable that the porous template 110 is laminated on the upper plate 121 so that the spatial region formed by the plurality of first air channels 122 may be blocked.

As shown in FIG. 4B, the first liquid injection step S20 may be performed by filling a space 111 of the porous template 110 with a first liquid, which is a source of a droplet to be generated .

For reference, the porous template 110 is preferably made of a material that does not mix with the first liquid, that is, a material that does not absorb the first liquid. If the first liquid is water, the porous template 110 is made of a hydrophobic material.

When the first liquid is injected into the space 111, the first liquid does not flow into the space formed by the plurality of microwells 112 of the porous template 110, The spaces formed by the space portions 111 of the plurality of micro wills 112 are filled. When the first liquid is injected into the space 111, the air inside the microwell 112 is trapped in the microwell 112.

As shown in FIG. 5 (a), no first liquid is present at the corners (or corners) of the microwell 112. The process of filling the corner (or corner) of the microwell 112 with the first liquid in this state will be described later.

The arrangement and fixing step S30 is a step in which the first liquid filled in the space 111 flows into the plurality of microwells 112 and is fixed and arranged on the porous template 110. [

In the arranging and fixing step S30, the vacuum pump 129 is driven to suck and remove the air present in the plurality of microwells 112, and the first liquid To the space formed by the microwell 112.

4 (c), the air existing in the microwell 112 flows through the lower part of the porous template 110 to the upper plate 121 Through the first air passage 122 formed in the first air passage 122. The air having passed through the first air passage 122 passes through the second air passage 124 formed in the connecting member 125 and passes through the suction hole 127 to the outside of the vacuum suction unit 120 .

In addition, the first liquid filled in the space 111 flows into the space formed by the microwell 112 as shown in FIG. 5 (a). At this time, when the excess first liquid remaining in the plurality of microwells 112 remains in the space 111, the first liquid may be removed from the space 111 . If the first liquid not injected into the microwell 112 is present in the space portion 111, the cover portion 130 can not be mounted in the space portion 111, so that the excess first liquid is firstly removed, 130 are preferably mounted.

The second liquid injecting step S40 is a step of injecting a second liquid into the space portion 111 without mixing with the first liquid and having a surface tension smaller than the surface tension of the first liquid, To generate droplets of the first liquid filled in the plurality of microwells 112, respectively.

In the second liquid injecting step S40, the vibrating pump driven in the arranging and fixing step S30 is stopped, and as shown in FIG. 4 (d), the upper surface of the porous template 110 The second liquid can be injected into the space portion 111 through the injection hole 131 formed in the cover portion 130 after the cover portion 130 is seated.

When the second liquid flows into the space 111 through the injection hole 131, the second liquid flows into the plurality of microwells 112 filled with the first liquid.

5A, the microwells 112 filled with the first liquid do not become wet by the first liquid and fine air remains in the lower corners or corners of the microwells 112. As shown in FIG. In this state, when the second liquid flows into the space 111, a difference in interfacial tension occurs between the first liquid and the second liquid, and as shown in FIG. 5 (b) The contact angle CA of the liquid becomes large. The capillary action causes the contact line of the first liquid and the second liquid to descend along the vertical wall surface of the microwell 112.

The contact line continues to move horizontally along the bottom surface of the microwell 112 through the lower edge or corner of the microwell 112. Accordingly, the first liquid filled in the microwell 112 can be generated as a sphere-type droplet as shown in FIG. 5 (c).

Generally, in the case of a wetted corner of a microwell, the contact line of the first liquid and the second liquid is stopped at the bottom corner (wet corner) of the microwell. Accordingly, an additional force, for example, a laplace pressure difference, is needed to move the contact line of the first liquid and the second liquid horizontally. However, in the high-density micro-droplet generation arranging system and method according to the embodiment of the present invention, when the first liquid is introduced into the microwell 112, the non-wetting corner (not shown) formed at the corner of the bottom of the microwell 112 the contact line between the first liquid and the second liquid can be moved horizontally along the bottom surface of the microwell 112 without being fixed at the bottom corner of the microwell. In this process, the difference in interfacial tension between the first liquid and the second liquid is used.

Therefore, high-density droplets can be arranged and fixed on the porous template 110. [

For example, in the embodiment of the present invention, spherical droplets are generated and arranged and fixed in a plurality of microwells 112 of the porous template 110, but the present invention is not limited thereto, A droplet of the dome may be generated and arranged and fixed.

7 is a graph showing the percentage of droplets generated according to aspect ratios (AR) of microwells. Where the aspect ratio AR is defined as the ratio of the length L to the height H (H / L). As shown in FIG. 7, a droplet having a spherical shape is generated as the aspect ratio is lower, and a dome-shaped droplet is generated as the aspect ratio is higher. Accordingly, the size and shape of the droplet can be easily changed by adjusting the aspect ratio of the microwell, that is, the length of the vertical surface and the bottom surface forming the microwell.

A high density microdroplet generating array system and method according to embodiments of the present invention can be used for real-time monitoring of a target material, a multi-probe sensor, a digital counting assay, Can be used.

The high density microdroplet generating array system and method according to the embodiment of the present invention is not only used in the technical field of curing and using droplets but also in a technical field in which droplets are used in arrangement and fixed state without curing, Both can be used.

While the present invention has been described in connection with what is presently considered to be practical exemplary embodiments, it is to be understood that the invention is not limited to the disclosed embodiments.

Therefore, the scope of the present invention should not be limited by the described embodiments, but should be determined by the scope of the appended claims and equivalents thereof.

110: Porous template 111:
112: Microwell 120: Vacuum suction part
121: upper plate 122: first air passage
123: lower plate 124: second air passage
125: connecting member 130: cover part
CA: contact angle

Claims (13)

A porous template in which a first liquid is filled, and a plurality of microwells connected to the space in a communicable manner;
A vacuum suction unit provided below the porous template for allowing a first liquid filled in the space to flow into the microwell; And
And a cover portion provided on the porous template and having an injection hole into which a second liquid not mixed with the first liquid flows,
Wherein the surface tension of the first liquid is greater than the surface tension of the second liquid.
The method according to claim 1,
Wherein the second liquid has a greater wettability with respect to the porous template than the first liquid.
The method according to claim 1,
The vacuum suction unit
An upper plate on which the porous template is placed and on which a plurality of first air flow paths are formed at positions corresponding to the porous template;
A lower plate provided below the upper plate;
A connection member which is disposed between the upper plate and the lower plate and connects the upper plate and the lower plate and has a second air flow path communicably connected to the plurality of first air flow paths; And
A vacuum pump connected to a suction hole formed in any one of the upper plate and the lower plate so as to communicate with the second air passage;
Density micro-droplet generation array system.
The method of claim 3,
Wherein the second air passage
A first opening forming a region corresponding to a region formed by the plurality of first air flow paths; And
A second opening portion having one end connected to the first opening portion and the other end extending in a direction in which the suction hole is formed and communicably connected to the suction hole;
Density micro-droplet generation array system.
The method of claim 3,
Wherein an adhesive material is applied to both sides of the connecting member.
The method according to claim 1,
The droplet of the first liquid is fixedly arranged in the microwell,
Wherein the droplet is fixedly arranged on the microwell in a spherical or dome shape.
7. The method according to any one of claims 1 to 6,
The porous template may be a porous template,
Wherein a contact line between the first liquid and the second liquid moves in a vertical direction along a sidewall surface of the microwell and then moves in a horizontal direction along a bottom surface of the microwell. Array system.
9. A method of high density microdroplet generation using a system according to claim 7,
A lamination step of laminating the porous template on an upper plate of the vacuum suction part;
A first liquid injection step of filling the space portion of the porous template stacked in the stacking step with the first liquid;
Arranging and fixing the first liquid filled in the space part in the first liquid injecting step into the microwell of the porous template; And
A second liquid injection step of injecting a second liquid into the space portion of the porous template;
Density micro-droplet generation array.
9. The method of claim 8,
In the arranging and fixing step,
Wherein the vacuum pump is driven to suck and remove the air present in the plurality of microwells and to introduce the first liquid filled in the space into the space in which the microwells are formed, .
10. The method of claim 9,
In the arranging and fixing step,
Wherein the first liquid is removed from the space when excess first liquid remaining in the plurality of microwells is left in the space.
10. The method of claim 9,
Wherein air is present in the lower edge or corner of the microwell filled with the first liquid.
9. The method of claim 8,
In the second liquid injection step,
And a second liquid having a surface tension smaller than the surface tension of the first liquid is injected into the space portion without being mixed with the first liquid to generate droplets of the first liquid in the plurality of microwells High density microdroplet generating array method.
13. The method of claim 12,
When the second liquid flows into the space, a difference in interfacial tension occurs between the first liquid and the second liquid,
Wherein the contact line of the first liquid and the second liquid is moved along a horizontal plane of the microwell via a vertical wall surface and a lower edge or corner of the microwell.
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KR20170003177A (en) * 2015-06-30 2017-01-09 (주) 마이크로핏 Polygons microplate, preparation mehthod thereof and culture method of cell aggregation using the same
KR20190061672A (en) 2017-11-28 2019-06-05 한국표준과학연구원 Droplet transfer apparatus and Droplet transfer method for using the droplet transfer apparatus
KR20190061712A (en) * 2017-11-28 2019-06-05 인제대학교 산학협력단 A Microfluidic Device for degassing in channel using a porous film
KR102030284B1 (en) * 2018-09-13 2019-10-08 인제대학교 산학협력단 A microfluidic device for degassing in channel using protruding support pattern on porous film and Method thereof
KR20210158139A (en) * 2020-06-23 2021-12-30 인제대학교 산학협력단 A Disposable Micro Fluidic Device

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011141299A (en) 2011-04-25 2011-07-21 Nippon Telegr & Teleph Corp <Ntt> Flow cell
US20120325665A1 (en) 2011-06-03 2012-12-27 The Regents Of The University Of California Microfluidic devices with flexible optically transparent electrodes
KR20130013537A (en) * 2011-07-28 2013-02-06 고려대학교 산학협력단 Surface tension induced concave microwell fabrication and cell aggregation using the same
KR20130101284A (en) * 2012-03-05 2013-09-13 한국과학기술원 Optoelectrofluidic control device integrated with hydrogel microwell arrays and manipulation method of microparticle and microdroplet using the same

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011141299A (en) 2011-04-25 2011-07-21 Nippon Telegr & Teleph Corp <Ntt> Flow cell
US20120325665A1 (en) 2011-06-03 2012-12-27 The Regents Of The University Of California Microfluidic devices with flexible optically transparent electrodes
KR20130013537A (en) * 2011-07-28 2013-02-06 고려대학교 산학협력단 Surface tension induced concave microwell fabrication and cell aggregation using the same
KR20130101284A (en) * 2012-03-05 2013-09-13 한국과학기술원 Optoelectrofluidic control device integrated with hydrogel microwell arrays and manipulation method of microparticle and microdroplet using the same

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20170003177A (en) * 2015-06-30 2017-01-09 (주) 마이크로핏 Polygons microplate, preparation mehthod thereof and culture method of cell aggregation using the same
KR101718920B1 (en) 2015-06-30 2017-03-23 (주) 마이크로핏 Polygons microplate, preparation mehthod thereof and culture method of cell aggregation using the same
KR20190061672A (en) 2017-11-28 2019-06-05 한국표준과학연구원 Droplet transfer apparatus and Droplet transfer method for using the droplet transfer apparatus
KR20190061712A (en) * 2017-11-28 2019-06-05 인제대학교 산학협력단 A Microfluidic Device for degassing in channel using a porous film
KR102030254B1 (en) 2017-11-28 2019-10-08 인제대학교 산학협력단 A Microfluidic Device for degassing in channel using a porous film
KR102030284B1 (en) * 2018-09-13 2019-10-08 인제대학교 산학협력단 A microfluidic device for degassing in channel using protruding support pattern on porous film and Method thereof
KR20210158139A (en) * 2020-06-23 2021-12-30 인제대학교 산학협력단 A Disposable Micro Fluidic Device
KR102451829B1 (en) 2020-06-23 2022-10-06 인제대학교 산학협력단 A Disposable Micro Fluidic Device

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