KR101386391B1 - Filter for removing a white corpuscle and method of manufacturing the same - Google Patents
Filter for removing a white corpuscle and method of manufacturing the same Download PDFInfo
- Publication number
- KR101386391B1 KR101386391B1 KR1020080081768A KR20080081768A KR101386391B1 KR 101386391 B1 KR101386391 B1 KR 101386391B1 KR 1020080081768 A KR1020080081768 A KR 1020080081768A KR 20080081768 A KR20080081768 A KR 20080081768A KR 101386391 B1 KR101386391 B1 KR 101386391B1
- Authority
- KR
- South Korea
- Prior art keywords
- polyvinyl alcohol
- chitosan
- leukocyte removal
- removal filter
- nanofiber web
- Prior art date
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
- A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
- A61M1/3621—Extra-corporeal blood circuits
- A61M1/3627—Degassing devices; Buffer reservoirs; Drip chambers; Blood filters
- A61M1/3633—Blood component filters, e.g. leukocyte filters
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
- A61M1/02—Blood transfusion apparatus
- A61M1/0281—Apparatus for treatment of blood or blood constituents prior to transfusion, e.g. washing, filtering or thawing
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
- A61M1/34—Filtering material out of the blood by passing it through a membrane, i.e. hemofiltration or diafiltration
- A61M1/3496—Plasmapheresis; Leucopheresis; Lymphopheresis
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D39/00—Filtering material for liquid or gaseous fluids
- B01D39/14—Other self-supporting filtering material ; Other filtering material
- B01D39/16—Other self-supporting filtering material ; Other filtering material of organic material, e.g. synthetic fibres
- B01D39/1607—Other self-supporting filtering material ; Other filtering material of organic material, e.g. synthetic fibres the material being fibrous
- B01D39/1615—Other self-supporting filtering material ; Other filtering material of organic material, e.g. synthetic fibres the material being fibrous of natural origin
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D39/00—Filtering material for liquid or gaseous fluids
- B01D39/14—Other self-supporting filtering material ; Other filtering material
- B01D39/16—Other self-supporting filtering material ; Other filtering material of organic material, e.g. synthetic fibres
- B01D39/1607—Other self-supporting filtering material ; Other filtering material of organic material, e.g. synthetic fibres the material being fibrous
- B01D39/1623—Other self-supporting filtering material ; Other filtering material of organic material, e.g. synthetic fibres the material being fibrous of synthetic origin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M2202/00—Special media to be introduced, removed or treated
- A61M2202/04—Liquids
- A61M2202/0413—Blood
- A61M2202/0439—White blood cells; Leucocytes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M2205/00—General characteristics of the apparatus
- A61M2205/02—General characteristics of the apparatus characterised by a particular materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M2205/00—General characteristics of the apparatus
- A61M2205/02—General characteristics of the apparatus characterised by a particular materials
- A61M2205/0244—Micromachined materials, e.g. made from silicon wafers, microelectromechanical systems [MEMS] or comprising nanotechnology
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M2205/00—General characteristics of the apparatus
- A61M2205/75—General characteristics of the apparatus with filters
- A61M2205/7545—General characteristics of the apparatus with filters for solid matter, e.g. microaggregates
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M2207/00—Methods of manufacture, assembly or production
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2239/00—Aspects relating to filtering material for liquid or gaseous fluids
- B01D2239/02—Types of fibres, filaments or particles, self-supporting or supported materials
- B01D2239/0216—Bicomponent or multicomponent fibres
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2239/00—Aspects relating to filtering material for liquid or gaseous fluids
- B01D2239/02—Types of fibres, filaments or particles, self-supporting or supported materials
- B01D2239/025—Types of fibres, filaments or particles, self-supporting or supported materials comprising nanofibres
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2239/00—Aspects relating to filtering material for liquid or gaseous fluids
- B01D2239/06—Filter cloth, e.g. knitted, woven non-woven; self-supported material
- B01D2239/0604—Arrangement of the fibres in the filtering material
- B01D2239/0618—Non-woven
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2239/00—Aspects relating to filtering material for liquid or gaseous fluids
- B01D2239/06—Filter cloth, e.g. knitted, woven non-woven; self-supported material
- B01D2239/0604—Arrangement of the fibres in the filtering material
- B01D2239/0631—Electro-spun
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2239/00—Aspects relating to filtering material for liquid or gaseous fluids
- B01D2239/06—Filter cloth, e.g. knitted, woven non-woven; self-supported material
- B01D2239/065—More than one layer present in the filtering material
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2239/00—Aspects relating to filtering material for liquid or gaseous fluids
- B01D2239/10—Filtering material manufacturing
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2239/00—Aspects relating to filtering material for liquid or gaseous fluids
- B01D2239/12—Special parameters characterising the filtering material
- B01D2239/1233—Fibre diameter
Landscapes
- Health & Medical Sciences (AREA)
- Heart & Thoracic Surgery (AREA)
- Vascular Medicine (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Anesthesiology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Engineering & Computer Science (AREA)
- Public Health (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Cardiology (AREA)
- Nonwoven Fabrics (AREA)
- Filtering Materials (AREA)
Abstract
본 발명은 백혈구 제거용 필터 및 그의 제조방법에 관한 것으로서, 평균직경이 100~2,000㎚이고 상온에서 물에 녹지 않는 키토산-폴리비닐알코올 복합 나노섬유들로 구성되며, 상기 키토산-폴리비닐알코올 복합 나노섬유들 사이에 평균직경이 0.5~3㎛인 공극들이 형성되어 있고, 두께가 0.5~50㎛인 키토산-폴리비닐알코올 복합 나노섬유 웹(A)층을 포함하는 것을 특징으로 한다.The present invention relates to a leukocyte removal filter and a method for manufacturing the same, consisting of chitosan-polyvinyl alcohol composite nanofibers having an average diameter of 100-2,000 nm and insoluble in water at room temperature, and the chitosan-polyvinyl alcohol composite nano Pores having an average diameter of 0.5 to 3 µm are formed between the fibers, and the chitosan-polyvinyl alcohol composite nanofiber web (A) layer having a thickness of 0.5 to 50 µm is characterized in that it comprises a layer.
본 발명에 따른 백혈구 제거용 필터는 백혈구 제거율이 높고, 혈소판의 점착 현상이 적어 혈액 흐름성, 여과효율 및 혈액적합성이 우수하고, 적혈구 또는 혈소판의 회수효율이 높고, 필터 여재의 표면 친수화 가공단계를 생략할 수 있어서 제조공정이 간소화되고, 수분에 의한 필터재의 붕괴가 없고, 전기방사시 물을 용매로 사용하므로 수혈 안정성이 우수한 장점이 있다.The leukocyte removal filter according to the present invention has a high leukocyte removal rate, little adhesion of platelets, excellent blood flowability, filtration efficiency and blood compatibility, high recovery efficiency of red blood cells or platelets, and surface hydrophilization processing step of filter media. Since it can be omitted, the manufacturing process is simplified, there is no collapse of the filter material due to moisture, and water is used as a solvent during electrospinning.
필터, 백혈구, 나노섬유, 부직포, 폴리비닐알코올, 전기방사. Filters, white blood cells, nanofibers, nonwoven, polyvinyl alcohol, electrospun.
Description
본 발명은 백혈구 제거용 필터 및 그의 제조방법에 관한 것으로서, 보다 구체적으로는 백혈구 제거율이 높고, 혈소판의 점착 현상이 적어 혈액 흐름성, 여과효율 및 혈액적합성이 우수하고, 적혈구 또는 혈소판의 회수효율이 높고, 필터 여재의 표면 친수화 가공단계를 생략할 수 있어서 제조공정이 간소화되고, 수분에 의한 필터재의 붕괴가 없고, 전기방사시 물을 용매로 사용하므로 수혈 안정성이 우수한 백혈구 제거용 필터 및 그의 제조방법에 관한 것이다.The present invention relates to a leukocyte removal filter and a method for manufacturing the same, more specifically, leukocyte removal rate is high, platelet adhesion is less, blood flow, filtration efficiency and blood compatibility is excellent, recovery efficiency of red blood cells or platelets It is possible to omit the surface hydrophilization processing step of the filter medium, and the manufacturing process is simplified, there is no collapse of the filter material due to moisture, and water is used as a solvent during electrospinning, and thus the leukocyte removal filter having excellent transfusion stability and its manufacture It is about a method.
백혈구제거필터는 수혈 부작용 억제를 위한 가장 적극적인 방법으로 일본, 미국, 유럽을 중심으로 사용의무화 추세에 있다.The leukocyte removal filter is the most aggressive method for inhibiting the side effects of transfusion, and has become mandatory in Japan, USA and Europe.
종래의 백혈구제거필터는 주로 폴리에스테르계 폴리머에 의한 부직포로 구성되어 있다. 그러나, 백혈구제거율과 적혈구 또는 혈소판 회수율을 높이기 위하여 보다 극세한 동시에 혈액적합성이 우수한 섬유에 의한 필터 여과층의 구성이 필요 하며, 일본의 도레이, 아사히 카세이 메디칼 가부시키가이샤, 미국의 e-Spin社 등에서 이에 대한 연구가 진행 중이다.The conventional leukocyte removal filter is mainly composed of a nonwoven fabric made of a polyester-based polymer. However, in order to increase the leukocyte removal rate and erythrocyte or platelet recovery rate, a filter filtration layer made of finer and blood compatible fibers is required.Toray, Asahi Kasei Medical Co., Ltd., e-Spin Co., Ltd. Research is ongoing.
종래의 혈액성분 분리필터는 대부분 태섬도의 섬유로 구성되는 부직포 층과 세섬도의 섬유로 구성되는 부직포 층으로 구성되어 있다. 또한, 대한민국 출원특허 제1997-0023052호 및 대한민국 출원특허 제1997-0023053호에서는 키토산 그라프트 중합체를 상기 종래의 혈액성분 분리필터에 도포함으로써 부직포 표면상의 양이온 잔기와 백혈구 사이의 정전기적 인력에 의하여 백혈구 제거효율을 높이는 기술을 제안하고 있다. 그러나 상기 방법은 키토산 고분자를 필터를 구성하는 섬유의 표면에 화학적인 처리로써 고정시키거나 혹은 키토산 콤플렉스를 부직포 제조과정에 투입하여 물리적으로 고정시키는 방법을 사용하여야 한다. 전자의 경우에는 화학물질을 사용함에 따라 혈액안전성이 떨어지는 단점이 있으며, 키토산 콤플렉스를 물리적인 방법으로 고정시키는 후자의 경우에는 키토산 콤플렉스를 별도로 제조해야한다는 번거로움이 따른다. 또한, 혈액성분 분리필터의 여재는 소수성이 강한 경우 혈소판의 점착 현상을 보이게 되므로 표면의 친수화가 필요하다. The conventional blood component separation filter is composed of a nonwoven fabric layer composed mainly of fibers of Taeseomdo and a nonwoven fabric layer composed of fibers of Sesido. In addition, the Korean Patent Application No. 1997-0023052 and the Korean Patent Application No. 1997-0023053 apply the chitosan graft polymer to the conventional blood component separation filter by the electrostatic attraction between the cationic moiety and the white blood cells on the surface of the nonwoven fabric A technique for increasing the removal efficiency is proposed. However, the method should be a method of fixing the chitosan polymer to the surface of the fiber constituting the filter by a chemical treatment or by physically fixing the chitosan complex in the nonwoven fabric manufacturing process. In the former case, there is a disadvantage in inferior blood safety due to the use of chemicals. In the latter case, the chitosan complex has to be prepared separately. In addition, the media of the blood component separation filter is hydrophobic because the surface of the platelet adhesion phenomenon is required when the hydrophobicity is strong.
또 다른 종래기술로서 대한민국특허 출원번호 1997-0025053에서는 키토산과 혈액 친화성 고분자를 동시에 상기 부직포 표면에 그라프트시키는 방법을 제안하고 있으나, 이와 같은 방법은 폴리알킬렌옥사이드 등의 친수성 고분자를 필터 구성 섬유의 표면에 고정시켜야 하는 어려움이 있다.As another conventional technology, Korean Patent Application No. 1997-0025053 proposes a method of simultaneously grafting chitosan and blood-compatible polymers on the surface of the nonwoven fabric, but such a method comprises filtering a hydrophilic polymer such as polyalkylene oxide into a filter fiber There is a difficulty to fix it on the surface.
본 발명은 이와 같은 종래의 문제점들을 해소할 수 있도록 평균직경이 100~2,000㎚인 키토산-폴리비닐알코올 복합 나노섬유(이하 "복합 나노섬유"라고 약칭한다)들로 구성된 복합 나노섬유 웹을 포함하여 백혈구 제거율이 높고, 혈소판의 점착 현상이 적어 혈액 흐름성, 여과효율 및 혈액적합성이 우수하고, 적혈구 또는 혈소판의 회수효율이 높고, 필터 여재의 표면 친수화 가공단계를 생략할 수 있어서 제조공정이 간소화되고, 수분에 의한 필터재의 붕괴가 없고, 전기방사시 물을 용매로 사용하므로 수혈 안정성이 우수한 백혈구 제거용 필터 및 그의 제조방법을 제공하고자 한다.The present invention includes a composite nanofiber web composed of chitosan-polyvinyl alcohol composite nanofibers (hereinafter, abbreviated as "composite nanofibers") having an average diameter of 100 to 2,000 nm to solve these problems. High leukocyte removal rate, low platelet adhesion, excellent blood flow, filtration efficiency and blood compatibility, high red blood cell or platelet recovery efficiency, omit the surface hydrophilization processing step of filter media, simplifying the manufacturing process The present invention is intended to provide a filter for removing leukocytes and a method for producing the same having excellent transfusion stability because water is used as a solvent during electrospinning without disintegration of the filter material due to moisture.
이와 같은 과제들을 달성하기 위한 본 발명의 백혈구 제거용 필터재는 평균직경이 100~2,000㎚이고 상온에서 물에 녹지 않는 키토산-폴리비닐알코올 복합 나노섬유들로 구성되며, 상기 키토산-폴리비닐알코올 복합 나노섬유들 사이에 평균직경이 0.5~3㎛인 공극들이 형성되어 있고, 두께가 0.5~50㎛인 키토산-폴리비닐알코올 복합 나노섬유 웹(A)층을 포함하는 것을 특징으로 한다.The leukocyte removal filter material of the present invention for achieving the above problems is composed of chitosan-polyvinyl alcohol composite nanofibers having an average diameter of 100-2,000 nm and insoluble in water at room temperature, and the chitosan-polyvinyl alcohol composite nano Pores having an average diameter of 0.5 to 3 µm are formed between the fibers, and the chitosan-polyvinyl alcohol composite nanofiber web (A) layer having a thickness of 0.5 to 50 µm is characterized in that it comprises a layer.
본 발명에 따른 백혈구 제거용 필터의 제조방법은 검화도가 90% 이상이며 중량 평균분자량이 10,000~1,500인 키토산 수지 20~80중량%와 검화도가 80% 이상인 폴리비닐알코올 수지 80~20중량%를 물에 5~40% 농도로 용해시켜 제조한 방사용액을 고전압이 걸려 있는 노즐(3)로 공급한 후, 상기 노즐(3)을 통해 노즐과 반대 전하를 갖는 고전압이 걸려 있는 컬렉터(4) 상에 전기방사하여 폴리비닐알코올을 나노섬유들로 이루어진 나노섬유 웹을 제조하는 공정; 및 제조된 나노섬유 웹을 160~200℃에서 2시간 이상 열처리하는 공정;을 포함하는 것을 특징으로 한다.The method for producing a leukocyte removal filter according to the present invention has a saponification degree of 90% or more and a weight average molecular weight of 10,000 to 1500, chitosan resin 20 to 80% by weight and saponification degree of 80% to 20% by weight of polyvinyl alcohol resin And supplying the spinning solution prepared by dissolving in a concentration of 5 to 40% in water to the nozzle (3) under high voltage, the collector (4) under high voltage having a charge opposite to the nozzle through the nozzle (3) Preparing a nanofiber web made of nanofibers with polyvinyl alcohol by electrospinning onto the substrate; And heat-treating the prepared nanofiber web at 160 to 200 ° C. for at least 2 hours.
본 발명에 따른 백혈구 제거용 필터는 상온에서는 물에 불용성이나 친수성이 높은 키토산-폴리비닐알코올 복합 나노섬유들로 이루어진 나노섬유 웹을 포함하기 때문에 백혈구 제거율이 높고, 혈소판의 점착 현상이 적어 혈액 흐름성, 여과효율 및 혈액적합성이 우수하고, 적혈구 또는 혈소판의 회수효율이 높고, 필터 여재의 표면 친수화 가공단계를 생략할 수 있어서 제조공정이 간소화되고, 수분에 의한 필터재의 붕괴가 없고, 전기방사시 물을 용매로 사용하므로 수혈 안정성이 우수하다.The leukocyte removal filter according to the present invention includes a nanofiber web made of chitosan-polyvinyl alcohol composite nanofibers, which is insoluble in water and high in hydrophilicity at room temperature, and thus has high leukocyte removal rate and low adhesion of platelets. It has excellent filtration efficiency and blood compatibility, high recovery efficiency of red blood cells or platelets, and can omit the surface hydrophilization processing step of the filter media, which simplifies the manufacturing process and does not collapse the filter material due to moisture. Since water is used as a solvent, the transfusion stability is excellent.
이하, 첨부한 도면 등을 통하여 본 발명을 상세하게 설명한다.Hereinafter, the present invention will be described in detail with reference to the accompanying drawings.
먼저, 본 발명에 따른 백혈구 제거용 필터는 상기의 키토산-폴리비닐알코올 복합 나노섬유들로 구성된 나노섬유 웹(이하 "복합 나노섬유 웹"이라고 한다)을 포함한다.First, the leukocyte removal filter according to the present invention includes a nanofiber web (hereinafter referred to as a "composite nanofiber web") composed of the chitosan-polyvinyl alcohol composite nanofibers.
본 발명은 한정된 필터 공간내에서 백혈구 세포와의 접촉기회를 높혀 백혈구 세포의 포집효율을 높히기 위해서 상기 나노섬유 웹(A)를 구성하는 나노섬유의 평 균직경을 100~2,000㎚로 가늘게 한다.The present invention narrows the average diameter of the nanofibers constituting the nanofiber web (A) to 100-2,000 nm in order to increase the contact chance with the white blood cells in the limited filter space to increase the collection efficiency of the white blood cells.
복합 나노섬유의 평균직경이 2,000㎚를 초과하는 경우에는 백혈구 세포의 포집효율이 떨어지고, 100㎚ 미만인 경우에는 나노섬유 간의 미세공극 크기가 너무 작아져 포집된 백혈구가 상기 미세공극을 막아 적혈구 또는 혈소판의 공급이 어렵게 된다.If the average diameter of the composite nanofibers exceeds 2,000nm, the collection efficiency of leukocytes is lowered, and if the average diameter of the composite nanofibers is less than 100nm, the micropore size between nanofibers is too small and the collected leukocytes block the micropores, thereby preventing red blood cells or platelets. Supply becomes difficult.
상기 복합 나노섬유 웹내에 형성된 공극의 평균직경은 0.5~3㎛, 보다 바람직하기로는 1.5~3㎛이다.The average diameter of the pores formed in the composite nanofiber web is 0.5-3 탆, more preferably 1.5-3 탆.
상기 공극의 평균직경이 3㎛를 초과하면 백혈구 세포의 포집효율이 떨어지게 되고, 0.5㎛ 미만인 경우에는 상기 공극이 포집된 백혈구에 의해 쉽게 막혀 적혈구 또는 혈소판의 공급이 어렵게 될 수 있다.If the average diameter of the pores is more than 3 mu m, the efficiency of collection of leukocyte cells is decreased. When the average diameter of the pores is less than 0.5 mu m, the pores may be easily clogged by the collected leukocytes and the supply of red blood cells or platelets may become difficult.
상기 복합 나노섬유 웹의 두께는 0.5~50㎛, 보다 바람직하기로는 2~10㎛이다.The composite nanofiber web has a thickness of 0.5 to 50 µm, more preferably 2 to 10 µm.
복합 나노섬유 웹의 두께가 상기 범위를 초과하는 경우에는 혈액통과에 따른 압력 손실이 너무 많아지고, 상기 범위 미만인 경우에는 백혈구 제거효율이 저하된다.When the thickness of the composite nanofiber web exceeds the above range, the pressure loss due to blood passage is too large, and when the thickness of the composite nanofiber web is below the above range, the leukocyte removal efficiency is lowered.
상기 키토산-폴리비닐알코올 복합 나노섬유는 키토산 20~80중량%와 폴리비닐알코올 수지 80~20중량%로 구성되고, 160~200℃에서 2시간 이상 열처리되어 상온에서 물에 불용성이나 친수성이 높다.The chitosan-polyvinyl alcohol composite nanofiber is composed of 20 to 80% by weight of chitosan and 80 to 20% by weight of polyvinyl alcohol resin, and is heat-treated at 160 to 200 ° C for at least 2 hours to insoluble in water or hydrophilic at room temperature.
한편, 본 발명은 상기 복합 나노섬유 웹(A)이 평균직경이 3~20㎛ 정도인 통상의 태섬도 합성섬유들로 이루어진 합성섬유 부직포(B) 상에 적층되거나, 상기 합 성섬유 부직포(B)들 사이에 적층되어 있는 구조를 포함한다.On the other hand, the present invention is the composite nanofiber web (A) is laminated on a synthetic fiber nonwoven fabric (B) made of conventional Taeseodo synthetic fibers having an average diameter of about 3 ~ 20㎛, or the synthetic fiber nonwoven fabric (B) It includes a structure stacked between the).
도 1은 본 발명에 따른 백혈구 제거용 필터 일례의 단면 모식도이다.1 is a schematic cross-sectional view of an example of a filter for removing leukocytes according to the present invention.
상기 합성섬유 부직포(B)는 평균직경이 2㎛를 초과하는 필라멘트들로 구성된 폴리에스테르 멜트브로운 부직포, 폴리에스테르 스펀본드 부직포 또는 폴리에스테르 니들펀칭 부직포 등이다.The synthetic fiber nonwoven fabric (B) is a polyester melt blown nonwoven fabric, a polyester spunbond nonwoven fabric or a polyester needle punched nonwoven fabric composed of filaments having an average diameter of more than 2 μm.
다음으로는 본 발명에 따른 백혈구 제거용 필터의 제조방법을 구체적으로 살펴본다.Next, a method of manufacturing a filter for removing leukocytes according to the present invention will be described in detail.
본 발명의 제조방법은 검화도가 90% 이상이며 중량 평균분자량이 10,000~1,500인 키토산 수지 20~80중량%와 검화도가 80% 이상인 폴리비닐알코올 수지 80~20중량%를 물에 5~40% 농도로 용해시켜 제조한 방사용액을 고전압이 걸려 있는 노즐(3)로 공급한 후, 상기 노즐(3)을 통해 노즐과 반대 전하를 갖는 고전압이 걸려 있는 컬렉터(4) 상에 전기방사하여 폴리비닐알코올을 나노섬유들로 이루어진 나노섬유 웹을 제조하는 공정; 및 제조된 나노섬유 웹을 160~200℃에서 2시간 이상 열처리하는 공정;을 포함하는 것을 특징으로 한다.In the preparation method of the present invention, 20 to 80% by weight of chitosan resin having a saponification degree of 90% or more and a weight average molecular weight of 10,000 to 1,500 and 80 to 20% by weight of polyvinyl alcohol resin having a saponification degree of 80% or more in water of 5 to 40 The spinning solution prepared by dissolving at a% concentration was supplied to the
상기 키토산-폴리비닐알코올 복합 나노섬유 웹(A)은 도 2에 도시된 전기방사 방식 등으로 제조할 수 있다.The chitosan-polyvinyl alcohol composite nanofiber web (A) can be prepared by the electrospinning method shown in FIG.
도 2는 본 발명에 포함된 키토산-폴리비닐알코올 복합 나노섬유 웹(A)을 전기방사 방식으로 제조하는 공정 개략도이다.Figure 2 is a schematic diagram of a process for producing a chitosan-polyvinyl alcohol composite nanofiber web (A) included in the present invention by an electrospinning method.
구체적으로, 방사액 주탱크(1) 내에 보관중인 키토산-폴리비닐알코올 혼합 수지의 방사용액을 계량펌프(2)를 사용하여 고전압이 걸려 있는 노즐(3)로 공급한 후, 상기 노즐(3)을 통해 방사용액을 고전압이 걸려 있는 컬렉터(4) 상으로 전기방사하여 키토산-폴리비닐알코올 복합 나노섬유를 형성하여, 이를 상기 컬렉터(4)에 적층시켜 키토산-폴리비닐알코올 복합 나노섬유 웹(A)을 제조한 다음, 상기 키토산-폴리비닐알코올 복합 나노섬유 웹(A)을 160~200℃에서 2시간 이상 열처리하여 도 3과 같은 키토산-폴리비닐알코올 복합 나노섬유 웹(A)을 제조한다.Specifically, the spinning solution of the chitosan-polyvinyl alcohol mixed resin stored in the spinning liquid main tank 1 is supplied to the
도 3은 본 발명에 포함된 키토산-폴리비닐알코올 복합 나노섬유 웹(A) 표면의 전자현미경 사진이다.3 is an electron micrograph of the surface of the chitosan-polyvinyl alcohol composite nanofiber web (A) included in the present invention.
상기 방사용액은 (ⅰ) 검화도가 80% 이상, 보다 바람직하기로는 90% 이상인 폴리비닐알코올 수지 20~80중량%와 (ⅱ) 검화도가 90% 이상이고 중량평균 분자량이 1만~150만인 수용성 키토산 80~20중량%를 75℃ 이상으로 가열된 증류수에 5~40 중량%의 농도로 용해시켜 제조한다.The spinning solution has a polyvinyl alcohol resin having a degree of saponification of 80% or more, more preferably 90% or more, and (ii) a degree of saponification of 90% or more and a weight average molecular weight of 10,000 to 1.5 million. 80 to 20% by weight of water-soluble chitosan is prepared by dissolving at a concentration of 5 to 40% by weight in distilled water heated to 75 ℃ or more.
상기 키토산의 중량평균 분자량이 1만 미만인 경우에는 키토산의 성질을 유지하지 못하고 당(糖)으로 변하게되고, 150만을 초과하면 분자량이 너무커서 수용성을 상실하게 된다.When the weight average molecular weight of the chitosan is less than 10,000, it does not maintain the properties of the chitosan and turns to sugar, and when it exceeds 1.5 million, the molecular weight is too large to lose water solubility.
또한, 키토산의 검화도가 90% 미만인 경우에는 혈액이 통과할 때 양이온 잔기의 양이 부족하여 백혈구를 끌어당기는 힘이 부족하여 여과효율이 저하될 수 있다.In addition, when the degree of saponification of chitosan is less than 90%, the amount of cationic residues is insufficient when blood flows, and thus, the ability to attract leukocytes may be insufficient, thereby lowering the filtration efficiency.
폴리비닐알코올의 검화도가 80% 미만인 경우에는 수용성이 높아서 방사용액의 제조는 용이하지만 나노섬유 제조후 열처리 공정으로 나노섬유를 불수용화 시키는 공정이 어렵게 된다.If the saponification degree of polyvinyl alcohol is less than 80%, the water solubility is high, so that the preparation of the spinning solution is easy, but the process of insolubilizing the nanofibers by the heat treatment process after the nanofiber production becomes difficult.
방사용액내 폴리비닐알코올의 농도가 5중량% 미만인 경우에는 전기방사시 나노섬유 형성능이 떨어져 비드(Bead) 발생 가능성이 높아지고, 40중량%를 초과할 경우에는 점도가 너무 높아 전기방사성이 저하될 수 있다.If the concentration of polyvinyl alcohol in the spinning solution is less than 5% by weight, nanofiber formation ability decreases during electrospinning, and thus, beads are more likely to be generated. If the concentration exceeds 40% by weight, the viscosity is too high and the electrospinning property may be reduced. have.
방사용액내 키토산의 함량이 20중량% 미만인 경우에는 필터의 친수성은 향상되나 여과공정에서 충분한 양이온 잔기가 형성되지 못하여 백혈구 제거 효율이 떨어지고, 80중량%를 초과하는 경우에는 백혈구 제거효율은 향상되나 필터의 친수성이 낮아져 혈소판의 침적현상이 심하게 발생될 수 있다.If the content of chitosan in the spinning solution is less than 20% by weight, the hydrophilicity of the filter is improved, but the leukocyte removal efficiency is decreased because sufficient cationic residues are not formed in the filtration process, and if it exceeds 80% by weight, the leukocyte removal efficiency is improved. Due to the low hydrophilicity of the platelet deposition may occur severely.
본 발명에서는 전기방사시 토출량, 전압, 분위기 온도, 습도 컬렉터와 노즐간의 거리, 노즐의 형태, 컬렉터의 이동속도 등은 특별하게 한정하지 않는다.In the present invention, the discharge amount, voltage, atmosphere temperature, distance between the humidity collector and the nozzle, the shape of the nozzle, and the moving speed of the collector during electrospinning are not particularly limited.
상기의 열처리공정은 전기방사된 폴리비닐알코올 나노섬유의 결정화도를 증가시켜 수용성을 낮추므로서 상온에서 불수용성이 되도록 하기 위해 실시하며, 열처리하는 방법에는 제한이 없으나, 균일한 열처리를 하기 위해서는 열풍에 의한 열처리가 바람직하다.The heat treatment process is carried out to increase the crystallinity of the electrospun polyvinyl alcohol nanofibers to reduce the water solubility so as to be insoluble at room temperature, and the method of heat treatment is not limited, but in order to uniform heat treatment to hot air Heat treatment is preferred.
상기 노즐(3)과 컬렉터(4)에는 전압전달로드(5)를 통해 전압발생장치(6)에서 발생되는 고전압을 걸어준다.The high voltage generated in the voltage generator 6 is applied to the
본 발명에서 사용하는 전기방사 장치에는 특별히 제한하지 않는다. 도 2에서 보는 바와 같은 다중 노즐을 사용하는 전기방사 장치를 사용할 수 있으며 이 외의 다른 형태의 전기방사 장치 또한 사용할 수 있다. 전기방사 장치는 고분자 방사 용액을 공급하는 계량 펌프(2)와 다수의 노즐(3)로 구성되는 방사부, 전압발생장치(6)에 의한 고전압발생부와 방사되어 휘산되는 나노섬유를 고착시키는 컬렉터(4)로 구성된다. 본 발명의 나노섬유를 방사하기 위한 발생전압은 수천 내지 수십만 볼트로 방사 용액의 농도, 계량 펌프를 통해 공급되는 방사 용액의 양, 얻고자 하는 나노섬유의 굵기 등을 고려하여 다양하게 적용할 수 있다.The electrospinning apparatus used in the present invention is not particularly limited. An electrospinning device using multiple nozzles as shown in FIG. 2 may be used, and other types of electrospinning devices may be used. The electrospinning device includes a radiator comprising a
상기와 같이 제조된 폴리비닐알코올을 나노섬유는 친수성이 우수하기 때문에 수혈과정에서 혈소판의 점착 현상으로 인해 별도의 친수화 공정을 생략할 수 있어서 공정이 간소화되며 혈액성분의 분리가 어렵게 되는 문제를 효과적으로 해결함과 동시에 상온에서 물에 녹지 않는 불용성이기 때문에 수혈시 수분에 의해 필터층이 붕괴되는 문제도 효과적으로 해결할 수 있다.Since the nanofiber of the polyvinyl alcohol prepared as described above has excellent hydrophilicity, a separate hydrophilization process can be omitted due to the adhesion of platelets in the transfusion process, which simplifies the process and makes it difficult to separate blood components effectively. At the same time, since it is insoluble in water at room temperature, it can be effectively solved the problem that the filter layer is collapsed by water during transfusion.
또한 본 발명은 전기방사시 유기용매 대신에 물을 사용하기 때문에 수혈 안정성이 우수하고 친환경적인 장점을 갖는다.In addition, since the present invention uses water instead of an organic solvent during electrospinning, it has excellent transfusion stability and environmentally friendly advantages.
본 발명에 따른 백혈구 제거용 필터(A)를 제조하는 방법 중 일례를 살펴보면, 앞에서 설명한 통상의 합성섬유 부직포(B) 상에 접착제를 도포 또는 스프레이(Spray)한 다음 앞에서 설명한 전기방사 방식으로 제조한 나노섬유 웹(A)을 접착제가 도포 또는 스프레이된 상기 합성섬유 부직포(B) 위에 겹쳐지게 올려준 다음, 이들을 열로울러 등으로 가열, 압착하여 필터를 제조할 수 있다.Looking at one example of a method for producing a leukocyte removal filter (A) according to the present invention, by applying or spraying the adhesive on the conventional synthetic fiber nonwoven fabric (B) described above and then prepared by the electrospinning method described above The nanofiber web (A) may be placed on the synthetic fiber nonwoven fabric (B) coated with an adhesive or sprayed thereon, and then heated and compressed with a heat roller or the like to prepare a filter.
또한, 도 2의 전기방사 장치 중 컬렉터(4) 위를 통과하는 합성섬유 부직포(B) 상에 키토산-폴리비닐알코올 복합 나노섬유를 전기방사 방식으로 적층한 후 이들을 열로울러 등으로 가열, 압착하여 필터를 제조할 수도 있다.In addition, the chitosan-polyvinyl alcohol composite nanofibers were laminated on the synthetic fiber nonwoven fabric B passing through the collector 4 in the electrospinning apparatus of FIG. 2 by electrospinning, and then heated and compressed by a heat roller or the like. Filters may also be prepared.
본 발명에 따른 백혈구 제거용 필터는 ASTM F 2149-01 방법으로 측정한 백혈구 제거율과 적혈구 회수율이 각각 90% 이상과 85% 이상이고, ASTM F 756 방법으로 측정한 용혈율(적혈구 파괴율)이 10% 이하이고, 아래방법으로 측정한 흡수높이가 2~10㎝ 이다.The leukocyte removal filter according to the present invention has a leukocyte removal rate and an erythrocyte recovery rate of 90% or more and 85% or more, respectively, measured by ASTM F 2149-01 method, and a hemolysis rate (erythrocyte destruction rate) of 10% or more, measured by ASTM F 756 method. It is% or less and the absorption height measured by the following method is 2-10 cm.
흡수높이 측정방법How to measure absorption height
길이가 10㎝이고 폭이 1㎝인 필터(샘플)를 25℃물에 1㎝ 깊이로 담근 후 물의 흡수높이가 최대가 될 때까지 방치하여 상기의 최대 흡수높이를 흡수높이로 한다. 흡수높이를 육안으로 쉽게 확인할 수 있도록 물에 색소를 첨가하는 것이 바람직하다.A filter (sample) having a length of 10 cm and a width of 1 cm is soaked at 25 ° C in water at a depth of 1 cm, and then the water is allowed to stand until its absorption height reaches a maximum. It is preferable to add a dye to the water so that the absorption height can be visually confirmed easily.
이하, 실시예를 통하여 본 발명을 상세하게 설명한다.Hereinafter, the present invention will be described in detail by way of examples.
그러나, 하기 실시예는 본 발명의 일례를 나타내는 것으로서, 본 발명의 보호범위가 하기 실시예로만 한정되는 것은 아니다.However, the following examples are illustrative of the present invention, and the scope of protection of the present invention is not limited to the following examples.
실시예Example 1 One
검화도가 90% 이상이고 중량평균 분자량이 20,000인 키토산과 검화도가 85%인 폴리비닐알코올 수지를 80:20의 중량비율로 90℃로 가열된 증류수에 10%(w/w)의 농도로 용해시켜 방사용액을 제조하였다.A chitosan having a degree of saponification of 90% or more and a weight average molecular weight of 20,000 and a polyvinyl alcohol resin having a degree of 85% saponification in a concentration of 10% (w / w) in distilled water heated to 90 ° C. at a weight ratio of 80:20. It was dissolved to prepare a spinning solution.
상기 방사용액을 도 2에 도시된 전기방사장치의 계량펌프(2)를 통해 28,000볼트(V)의 전압이 걸려있는 노즐(3)을 통해 28,000볼트(V)의 전압이 걸려있는 컬렉터(4) 위를 통과하는 폴리에스테르 멜트 블로운 부직포(기재) 상에 전기방사하여 평균직경이 600㎚인 키토산-폴리비닐알코올 복합 나노섬유들이 5㎛의 두께로 적층 되어 있고, 평균 공극크기가 3㎛인 나노섬유 웹(A)을 형성한 다음, 계속해서 165℃의 열풍으로 3시간 동안 열처리하여 키토산-폴리비닐알코올 복합 나노섬유 웹(A)을 포함하는 2층 구조의 백혈구 제거용 필터를 제조하였다.Collector 4, the voltage of 28,000 volts (V) through the
상기 폴리에스테르 멜트 블로운 부직포(기재)는 평균직경이 3㎛인 폴리에스테르로 구성되며, 공극의 평균직경이 40㎛이였다.The polyester melt blown nonwoven fabric (substrate) was composed of polyester having an average diameter of 3 μm, and the average diameter of the voids was 40 μm.
제조된 백혈구 제거용 필터의 각종 물성을 평가한 결과는 표 2와 같았다.The results of evaluating various physical properties of the manufactured leukocyte removal filter were as shown in Table 2.
실시예Example 2 ~ 2 ~ 실시예Example 4 및 4 and 비교실시예Comparative Example 1 One
키토산-폴리비닐알코올 복합 나노섬유 웹(A)을 제조하는데 사용되는 방사용액의 제조조건과 열처리 온도를 표 1과 같이 변경한 것을 제외하고는 실시예 1과 동일하게 2층 구조의 백혈구 제거용 필터를 제조하였다.A filter for removing leukocytes having a two-layer structure in the same manner as in Example 1 except for changing the manufacturing conditions and heat treatment temperature of the spinning solution used to prepare the chitosan-polyvinyl alcohol composite nanofiber web (A) as shown in Table 1. Was prepared.
제조된 백혈구 제거용 필터의 각종물성을 평가한 결과는 표 2와 같았다.The results of evaluating various physical properties of the prepared leukocyte removal filter were shown in Table 2.
비교실시 2Comparative Example 2
실시예 1에서 기재로 사용한 폴리에스테르 멜트 블로운 부직포만으로 키토산-폴리비닐알코올 복합 나노섬유 웹(A)이 포함되지 않은 백혈구 제거용 필터를 제조하였다.A leukocyte removal filter containing no chitosan-polyvinyl alcohol composite nanofiber web (A) was prepared using only the polyester melt blown nonwoven fabric used as the substrate in Example 1.
제조된 백혈구 제거용 필터의 각종물성을 평가한 결과는 표 2와 같았다.The results of evaluating various physical properties of the prepared leukocyte removal filter were shown in Table 2.
division
열처리온도
(℃)
Heat treatment temperature
(° C)
(열처리 안함)0
(No heat treatment)
도 1은 본 발명에 따른 백혈구 제거용 필터 일례의 단면 모식도.1 is a schematic sectional view of an example of a filter for removing leukocytes according to the present invention.
도 2는 본 발명에 포함된 키토산-폴리비닐알코올을 복합 나노섬유 웹(A)을 전기방사 방식으로 제조하는 공정 개략도.Figure 2 is a schematic diagram of a process for producing a composite nanofiber web (A) of chitosan-polyvinyl alcohol included in the present invention.
도 3은 본 발명에 포함된 키토산-폴리비닐알코올 복합 나노섬유 웹(A) 표면의 전자현미경 사진.Figure 3 is an electron micrograph of the surface of the chitosan-polyvinyl alcohol composite nanofiber web (A) included in the present invention.
* 도면 중 주요부분에 대한 부호설명[0001] Description of the Related Art [0002]
A : 키토산-폴리비닐알코올을 복합 나노섬유 웹 A: composite nanofiber web with chitosan-polyvinyl alcohol
B: 합성섬유 부직포 1 : 방사액 주탱크 2 : 계량펌프B: Synthetic fiber nonwoven fabric 1: Spinning liquid main tank 2: Metering pump
3 : 노즐 4 : 컬렉터3: nozzle 4: collector
5 : 전압전달로드 6 : 전압발생장치5: voltage transfer rod 6: voltage generator
Claims (12)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020080081768A KR101386391B1 (en) | 2008-08-21 | 2008-08-21 | Filter for removing a white corpuscle and method of manufacturing the same |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020080081768A KR101386391B1 (en) | 2008-08-21 | 2008-08-21 | Filter for removing a white corpuscle and method of manufacturing the same |
Publications (2)
Publication Number | Publication Date |
---|---|
KR20100023150A KR20100023150A (en) | 2010-03-04 |
KR101386391B1 true KR101386391B1 (en) | 2014-04-18 |
Family
ID=42175436
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1020080081768A KR101386391B1 (en) | 2008-08-21 | 2008-08-21 | Filter for removing a white corpuscle and method of manufacturing the same |
Country Status (1)
Country | Link |
---|---|
KR (1) | KR101386391B1 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20200005846A (en) * | 2018-07-09 | 2020-01-17 | 한국생명공학연구원 | Method for manufacturing nanofiber membrane, nanofiber membrane and mask filter comprising the same |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102634930B (en) * | 2012-04-13 | 2015-09-02 | 武汉纺织大学 | A kind of filter material containing polymer nanofiber and preparation method thereof |
KR101427700B1 (en) * | 2012-06-29 | 2014-08-07 | 주식회사 아모그린텍 | Cytokine Adsorbent Sheet, Manufacturing Method Thereof and Blood Filter using The same |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP3172542B2 (en) | 1990-06-25 | 2001-06-04 | テルモ株式会社 | Filter material for capturing leukocytes and method for producing the same |
-
2008
- 2008-08-21 KR KR1020080081768A patent/KR101386391B1/en active IP Right Grant
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP3172542B2 (en) | 1990-06-25 | 2001-06-04 | テルモ株式会社 | Filter material for capturing leukocytes and method for producing the same |
Non-Patent Citations (1)
Title |
---|
논문, Carbohydrate polymers(2006.07.25) * |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20200005846A (en) * | 2018-07-09 | 2020-01-17 | 한국생명공학연구원 | Method for manufacturing nanofiber membrane, nanofiber membrane and mask filter comprising the same |
KR102139710B1 (en) | 2018-07-09 | 2020-07-30 | 한국생명공학연구원 | Method for manufacturing nanofiber membrane, nanofiber membrane and mask filter comprising the same |
Also Published As
Publication number | Publication date |
---|---|
KR20100023150A (en) | 2010-03-04 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN109219474B (en) | Filter medium, method for manufacturing the same, and filter module including the same | |
CN107923092B (en) | Nanofiber for filter media, filter media comprising same, preparation method thereof and filter unit comprising same | |
CN109219475B (en) | Filter assembly, method of manufacturing the same, and filter module including the same | |
JP2013535314A (en) | Filter medium for liquid filter using electroradiated nanofiber web, manufacturing method thereof, and liquid filter using the same | |
KR101142853B1 (en) | Ultrafine polymeric fiber-based filter with improved heat resistance and preparation method thereof | |
US11364470B2 (en) | Filter medium, manufacturing method therefor, and filter unit comprising same | |
CN111905453A (en) | Self-supporting pleatable web and oil filter comprising same | |
US11207644B2 (en) | Filter medium with improved backwashing durability, method for manufacturing same, and filter unit comprising same | |
CN113646474A (en) | Composite structure, method for producing same, and filter containing same | |
KR101628898B1 (en) | Liquid Treating Chemical Filter Using Nano-Fiber Web Having Ion Exchange Resin Particle and Method of Manufacturing the Same | |
KR102576129B1 (en) | Filter media and Filter unit comprising the same | |
KR101628899B1 (en) | Liquid Treating Chemical Filter Using Sulfonated Nano-Fiber Web and Method of Manufacturing the Same | |
WO2019058292A1 (en) | Nano-fiber based filter media and methods of preparation thereof | |
KR101691636B1 (en) | Ultrafine fiber-based filter with super-flux and high filtration efficiency and preparation method thereof | |
KR101452251B1 (en) | Filter for removing a white corpuscle and method of manufacturing the same | |
KR101386391B1 (en) | Filter for removing a white corpuscle and method of manufacturing the same | |
KR101619235B1 (en) | Liquid Chemical Filter Using Nano-Fiber Web by Electrospinning and Method of Manufacturing the Same | |
KR102576134B1 (en) | Filter media and Filter unit comprising the same | |
KR101386424B1 (en) | Filter for removing a white corpuscle and method of manufacturing the same | |
KR102563110B1 (en) | Nanofiber filter and preparation method thereof | |
KR102157444B1 (en) | Multy-Layer Structure Filter Medium For High-Performance Air Cleaning Filter | |
KR102064920B1 (en) | Filter media, method for manufacturing thereof and Filter unit comprising the same | |
KR102621800B1 (en) | Nanofiber filter and preparation method thereof | |
KR102680682B1 (en) | Preparation method of composite nanofiber filter | |
KR101921826B1 (en) | Flexible multi-porous multi-layered hydrophilic nanofiber membrane and manufacturing method thereof |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
N231 | Notification of change of applicant | ||
A201 | Request for examination | ||
E902 | Notification of reason for refusal | ||
E701 | Decision to grant or registration of patent right | ||
GRNT | Written decision to grant | ||
FPAY | Annual fee payment |
Payment date: 20190401 Year of fee payment: 6 |