KR101174360B1 - Apparatus for measuring ph and method thereof - Google Patents

Apparatus for measuring ph and method thereof Download PDF

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KR101174360B1
KR101174360B1 KR1020100120593A KR20100120593A KR101174360B1 KR 101174360 B1 KR101174360 B1 KR 101174360B1 KR 1020100120593 A KR1020100120593 A KR 1020100120593A KR 20100120593 A KR20100120593 A KR 20100120593A KR 101174360 B1 KR101174360 B1 KR 101174360B1
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microchannel
reaction
indicator
aqueous solution
sensitive material
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KR1020100120593A
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KR20120059006A (en
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배영민
이경희
손서영
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한국전기연구원
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Abstract

An apparatus and method for measuring PH according to the present invention are disclosed.
Apparatus for measuring the pH according to the present invention comprises a indicator microchannel in which the indicator solution is injected and the pH sensitive material is fixed at a predetermined position; a reaction microchannel in which a reaction aqueous solution or a target aqueous solution to measure pH is injected and a pH sensitive material is fixed at a predetermined position; And a connecting microchannel connecting the indicator microchannel and the reaction microchannel and a scale for checking pH according to the reaction of the indicator solution and the target aqueous solution on one side thereof.
Through this, the present invention can visually measure pH without the need for a separate reader, and can be applied to the biochip field because it uses a simple structure in which a microfluidic channel through which a fluid flows is present on a solid substrate.

Description

Apparatus for measuring pH and its method {APPARATUS FOR MEASURING PH AND METHOD THEREOF}

The present invention relates to a pH measuring apparatus, and in particular, when the aqueous solution flows inside the reaction microchannel in which the pH sensitive material is present at a constant flow rate, the volume of the pH sensitive material changes according to the pH of the aqueous solution, and the pH is changed according to the volume change. An apparatus and a method for measuring pH for measuring pH by measuring a change in pressure caused in a region where a sensitizer is present are disclosed.

pH is a measurement widely used in the chemical, biological and medical fields, and refers to a measurement of acidity or basicity in an aqueous solution. Various techniques have been developed to measure pH, and potentiometers are widely used.

In principle, the potentiometric method is to measure the potentiometric change of the sensitized film using a sensitized film that selectively binds to the hydrogen ions contained in the aqueous solution to change the potential difference at the interface. To this end, it is composed of a sensitive film and an internal electrode, a reference electrode, and a reader for measuring such a film potential difference.

In addition, recently, a sensor in which an internal electrode, a sensitive film, and a reference electrode are compactly developed has been developed, which is used by connecting an external reader and a wire.

However, since the pH measurement using the conventional potentiometric method is inevitably connected to the reader to check the pH value, there is a problem that you need to carry the reader in the field test outside the laboratory. In addition, even if a miniaturized sensor is used, there is a physical limitation to embed it in a biochip that is widely used in recent years.

Therefore, an object of the present invention was devised to solve the above problems, and when the aqueous solution flows inside the reaction microchannel in which the pH sensitive material is present at a constant flow rate, the volume of the pH sensitive material changes depending on the pH of the aqueous solution. The present invention provides an apparatus and a method for measuring pH to measure pH by measuring a change in pressure caused in a region where a pH sensitive material is present according to the volume change.

To this end, the device for measuring the pH according to another aspect of the present invention comprises an indicator microchannel in which the indicator solution is injected and the pH sensitive material is fixed at a predetermined position; a reaction microchannel in which a reaction aqueous solution or a target aqueous solution to measure pH is injected and a pH sensitive material is fixed at a predetermined position; And a connecting microchannel connecting the indicator microchannel and the reaction microchannel and a scale for checking pH according to the reaction of the indicator solution and the target aqueous solution on one side thereof.

Preferably, the indicator microchannel and the reaction microchannel have the same cross-sectional area, and the indicator liquid and the target aqueous solution are injected into the indicator microchannel and the reaction microchannel at a constant volume velocity.

Preferably, the pH sensitive material is fixed at a predetermined position of the indicating microchannel and fixed to a connecting portion to which the connecting microchannel is connected, and fixed to a predetermined position of the reaction microchannel, and the connecting microchannel is connected. It is characterized by being fixed to the site.

Preferably, the pH sensitive material is characterized in that its volume changes according to the pH of the indicator solution and the target aqueous solution.

If necessary, the pH sensitive material is characterized by using a hydrogel material.

Apparatus for measuring pH according to another aspect of the present invention is a reaction microchannel in which a reaction solution or a target aqueous solution to measure the pH is injected, the pH sensitive material is fixed at a predetermined position; And the indicator solution is injected, and connected to the reaction microchannel, a scale for checking the pH according to the reaction of the indicator solution and the target aqueous solution is displayed on one side, characterized in that it comprises an indicator microchannel.

Preferably, the indicating microchannel is connected to the reaction microchannel, and both ends thereof are connected to one side and the other side spaced by the same distance from the center of the reaction microchannel.

Preferably, the pH sensitive material is fixed to a predetermined position of the reaction microchannel, characterized in that the one of the connection sites to which the indicator microchannel is connected.

Preferably, the pH sensitive material is characterized in that its volume changes according to the pH of the indicator solution and the target aqueous solution.

If necessary, the pH sensitive material is characterized by using a hydrogel material.

Through this, the present invention, when the aqueous solution flows inside the reaction microchannel in which the pH sensitive material is present at a constant flow rate, the volume of the pH sensitive material is changed according to the pH of the aqueous solution, and the region in which the pH sensitive material is present according to the volume change. By measuring the pressure change that occurs at, there is an effect that the pH can be measured visually without the need for a separate reader.

In addition, the present invention has an effect that can be applied to the field of biochips because it uses a simple structure in which a microfluidic channel through which a fluid flows exists in a solid substrate.

1 is a first exemplary view showing an apparatus for measuring pH according to an embodiment of the present invention.
FIG. 2 is an exemplary diagram for describing an operating principle of the pH measuring apparatus shown in FIG. 1.
3 is a second exemplary view showing an apparatus for measuring pH according to an embodiment of the present invention.
4 is an exemplary view for explaining an operating principle of the pH measuring device shown in FIG.

Hereinafter, an apparatus and method for measuring pH according to an embodiment of the present invention will be described in detail with reference to FIGS. 1 to 4.

In particular, the present invention, when the aqueous solution flows into the reaction microchannel in which the pH sensitive material is present at a constant flow rate, not the potentiometric method, the volume of the pH sensitive material is changed depending on the pH of the aqueous solution. By measuring the change in pressure caused in the region where the material is present, this suggests a way to measure the pH.

1 is a first exemplary view showing an apparatus for measuring pH according to an embodiment of the present invention.

As shown in FIG. 1, the pH measurement apparatus according to the present invention includes a solid substrate 110, a reaction microchannel 120, an indicator microchannel 130, and formed to allow a fluid to flow on the solid substrate 110. And a connection microchannel 140.

The reaction microchannel 120 and the indicator microchannel 130 have the same cross-sectional area, and the reaction microchannel 120 is injected with the reaction aqueous solution or the target aqueous solution and the indicator microchannel 130 is injected with the indicator liquid. Aqueous solution and indicator liquid are injected at a constant volume rate.

The reaction microchannel 120 and the indicating microchannel 130 may be connected by the connection microchannel 140. In this case, the pH sensitive material may be fixed to the connection portion of the reaction microchannel 120 and the connection microchannel 140, and the pH sensitive material may be fixed to the connection portion of the indication microchannel 130 and the connection microchannel 140. have.

Here, the pH sensitive material may vary in volume depending on the pH of the indicator solution or the target aqueous solution, for example, it is preferable to use a hydrogel material. The hydrogel is formed of a network of hydrophilic polymers and can absorb moisture within the network to change its volume.

The change in the composition of the hydrogel can change the absorbency of the water according to the pH of the aqueous solution around the hydrogel, and as a result, the volume is changed according to the change of pH, the present invention uses this principle I would like to.

In the connecting microchannel 140, a scale 150 indicating a pH is displayed on one side, and the pH of the aqueous solution can be visually confirmed according to the reaction of the indicator liquid and the reaction aqueous solution.

FIG. 2 is an exemplary diagram for describing an operating principle of the pH measuring apparatus shown in FIG. 1.

As shown in FIG. 2, in the PH measuring apparatus according to the present invention, the indicating microchannel and the reaction microchannel having the same cross-sectional area are connected by connecting microchannels, and the same volume of pH sensitive material is fixed to each connecting portion. have. In addition, as an example of the pH sensitive material, it is possible to use a hydrogel increases in volume as the pH is increased.

Figure (a) shows the case of using the reaction solution and the indicator liquid with the same pH. The indicator liquid having a constant pH is injected into the indicator microchannel at a constant volume rate. The aqueous solution is injected at the same volume velocity as the indicator liquid. At this time, since the liquid of the same pH flows in each channel, the volume of the pH sensitive material in each channel is the same. Therefore, the pressure in the presence of the pH sensitive material is constant, the interface between each channel aqueous solution through the connecting portion is located in the center of the connecting microchannel.

Figure (b) shows that the pH of the reaction aqueous solution is higher than the pH of the indicator liquid. The volume of the pH-sensitive material immobilized on the reaction microchannel increases and the cross-sectional area of the local channel decreases. Accordingly, the linear velocity of the target aqueous solution maintaining a constant volume velocity is increased.

The relationship between the volume velocity and the linear velocity can be expressed as Equation 1 below.

[Equation 1]

Volume velocity = channel cross section x linear velocity

Thus, as the linear velocity increases, the pressure near the hydrogel in terms of hydrodynamics decreases. This phenomenon can be easily explained through Bernoulli's theorem. When the pressure in the vicinity of the hydrogel is lowered, the interface between the target aqueous solution of the connecting microchannel and the indicator liquid moves toward the lower pressure, that is, toward the reaction microchannel.

Figure (c) shows that the pH of the reaction aqueous solution is lower than the pH of the indicator liquid. The volume of the pH sensitive material immobilized on the reaction microchannel decreases and the cross-sectional area of the local channel increases. As a result, the linear velocity of the liquid maintaining a constant volume velocity decreases, and the pressure near the hydrogel increases.

As the pressure near the hydrogel increases, the interface between the target aqueous solution and the indicator liquid in the linking channel moves toward the lower pressure, that is, toward the indicator microchannel.

Thus, the pH measuring device according to the present invention can confirm the change in the position of the interface between the two aqueous solution of the connecting microchannel as the pH of the reaction aqueous solution by comparing the indicator liquid and the reaction aqueous solution through the scale displayed on the connecting microchannel As a result, the pH of the reaction aqueous solution or the target aqueous solution can be visually confirmed.

3 is a second exemplary view showing an apparatus for measuring pH according to an embodiment of the present invention.

As shown in FIG. 3, the pH measuring apparatus according to the present invention has a structure different from that of FIG. 1, in which the reaction microchannel 120 is formed to allow fluid to flow on the solid substrate 110 and the solid substrate 110. And the indicating microchannel 130.

The reaction microchannel 120 and the indicator microchannel 130 have different cross-sectional areas of the channels, and the reaction microchannel 120 and the indicator microchannel 130 are connected to each other by the same distance from the center of the reaction microchannel 120. The indication microchannel 130 may be connected to one side and the other side spaced apart from each other.

The pH sensitive material may be fixed to one of the connection portions of the reaction microchannel 120 and the indicator microchannel 130. An indicator liquid is present in the reaction microchannel 120, and an aqueous reaction solution or a target aqueous solution is injected into the reaction microchannel 120 at a constant volume velocity.

In the indication microchannel 140, a scale 150 indicating a pH is displayed on one side, and the pH of the aqueous solution may be visually confirmed according to the reaction of the indication liquid and the reaction aqueous solution.

4 is an exemplary view for explaining an operating principle of the pH measuring device shown in FIG.

As shown in FIG. 4, in the pH measuring apparatus according to the present invention, both ends of the indicating microchannel are connected to the reaction microchannel, and the pH sensitive material may be fixed at one of the connection sites of the reaction microchannel and the indicating microchannel. have. In addition, as an example of the pH sensitive material, it is possible to use a hydrogel increases in volume as the pH is increased.

When the aqueous solution to be measured pH is injected into the reaction microchannel at a constant volume rate, the cross-sectional area of one of the two connection sites of the reaction microchannel connected to the indicator microchannel due to the volume of the hydrogel is reduced, and accordingly, Local linear velocity differences occur. This linear velocity difference generates a pressure difference between the two connection sites by Bernoulli's theorem, which is represented by a change in the position of the indicator liquid in the indicator microchannel.

That is, in Figure (a) as a case of injecting the target aqueous solution having a pH value of pH0, the indicator solution is located in a specific position of the scale indicating the pH in the indicator fine channel 140.

In Figure (b), when the target aqueous solution having a pH less than pH0 is injected, the volume of the pH sensitive material immobilized on the reaction microchannel decreases, and the pH sensitive material is The pressure at the position is higher than the site without the pH sensitive material. As a result, the indicator solution located at pH 0 is positioned to the right in the indicator microchannel 140.

In Figure (c), when the target aqueous solution having a pH greater than pH0 is injected, the volume of the pH sensitive substance immobilized on the reaction microchannel increases, and the pH sensitive substance is The pressure at the location is lower than the site without the pH sensitive material. As a result, the indicator solution located at the pH of the indicator microchannel 140 is located to the left.

By confirming the positional change of such an indication liquid, pH of the target aqueous solution can be confirmed.

As described above, the present invention is that when the aqueous solution flows inside the reaction microchannel in which the pH sensitive material is present at a constant flow rate, the volume of the pH sensitive material is changed depending on the pH of the aqueous solution. By measuring the pressure change caused in the existing area, the pH can be measured visually without the need for a separate reader, and it is applied to the biochip field because it uses a simple structure in which a microfluidic channel through which a fluid flows on a solid substrate exists. You may.

Apparatus and method for measuring pH according to the present invention can be modified and applied in various forms within the scope of the technical idea of the present invention and are not limited to the above embodiments. In addition, the embodiments and drawings are merely for the purpose of describing the contents of the invention in detail, not intended to limit the scope of the technical idea of the invention, the present invention described above is common knowledge in the technical field to which the present invention belongs As those skilled in the art can have various substitutions, modifications, and changes without departing from the technical spirit of the present invention, it is not limited to the above embodiments and the accompanying drawings, of course, and not only the claims to be described below but also claims Judgment should be made including scope and equivalence.

110: solid substrate
120: reaction microchannel
130: indicating fine channel
140: connection microchannel
150: graduation

Claims (10)

An indicator microchannel in which an indicator solution is injected and a pH sensitive material is fixed at a predetermined position;
a reaction microchannel in which a reaction aqueous solution or a target aqueous solution to measure pH is injected and a pH sensitive material is fixed at a predetermined position; And
Connected microchannel connecting the indicator microchannel and the reaction microchannel and a scale for checking pH according to the reaction of the indicator solution and the target aqueous solution on one side thereof is displayed.
Apparatus for measuring pH comprising a. The method according to claim 1,
The indicator microchannel and the reaction microchannel have the same cross-sectional area, and the indicator liquid and the target aqueous solution are injected into the indicator microchannel and the reaction microchannel at a constant volume velocity. Device for. The method according to claim 1,
The pH sensitive material,
The fixed microchannel is fixed to a predetermined position, and the connection microchannel is fixed to a connection portion connected, and the reaction microchannel is fixed to a predetermined position, characterized in that the connection microchannel is fixed to a connection portion connected to the Device for measuring pH. The method according to claim 1,
The pH sensitive material,
Apparatus for measuring pH, characterized in that the volume changes in accordance with the pH of the indicator solution and the target aqueous solution The method according to claim 1,
The pH sensitive material,
Apparatus for measuring pH, characterized by using a hydrogel material. a reaction microchannel in which a reaction aqueous solution or a target aqueous solution to measure pH is injected and a pH sensitive material is fixed at a predetermined position; And
The indicator solution is injected, and connected to the reaction microchannel, a scale for checking pH according to the reaction between the indicator solution and the target aqueous solution is displayed on one side thereof, and the indicator microchannel is displayed.
Apparatus for measuring pH comprising a. The method of claim 6,
The indication microchannel is,
Is connected to the reaction microchannel, the device for measuring pH, characterized in that both ends are connected to one side and the other side spaced by the same distance from the center of the reaction microchannel. The method of claim 6,
The pH sensitive material,
Fixing at a predetermined position of the reaction microchannel, the apparatus for measuring pH, characterized in that fixed to one of the connection site to which the indicator microchannel is connected. The method of claim 6,
The pH sensitive material,
Apparatus for measuring pH, characterized in that the volume changes in accordance with the pH of the indicator solution and the target aqueous solution The method of claim 6,
The pH sensitive material,
Apparatus for measuring pH, characterized by using a hydrogel material.
KR1020100120593A 2010-11-30 2010-11-30 Apparatus for measuring ph and method thereof KR101174360B1 (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20190005401A (en) 2017-07-06 2019-01-16 한림대학교 산학협력단 Biosensor for measuring impedance, temperature and hydrogen ion concentration
KR20190112935A (en) 2018-03-27 2019-10-08 재단법인 대구경북첨단의료산업진흥재단 pH DETECTING MICRO-STRUCTURED STICKER AND THE MANUFACTURING METHOD THEREOF

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201317746D0 (en) 2013-10-08 2013-11-20 Smith & Nephew PH indicator
CN106687801A (en) 2014-07-10 2017-05-17 史密夫及内修公开有限公司 Improvements in and relating to polymer materials
GB201600746D0 (en) 2016-01-14 2016-03-02 Smith & Nephew Improvements in and relating to polymer materials
GB201600747D0 (en) 2016-01-14 2016-03-02 Smith & Nephew Improvements in and relating to devices

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Publication number Priority date Publication date Assignee Title
JPH10332634A (en) 1997-06-05 1998-12-18 Nippon Koden Corp Device and method for measuring substance causing ph change or living thing body
WO2007102292A1 (en) 2006-03-07 2007-09-13 Konica Minolta Medical & Graphic, Inc. Inspection equipment and inspection system employing microchip
JP2008026145A (en) 2006-07-21 2008-02-07 Univ Nihon Ph measuring instrument and ph measuring method

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH10332634A (en) 1997-06-05 1998-12-18 Nippon Koden Corp Device and method for measuring substance causing ph change or living thing body
WO2007102292A1 (en) 2006-03-07 2007-09-13 Konica Minolta Medical & Graphic, Inc. Inspection equipment and inspection system employing microchip
JP2008026145A (en) 2006-07-21 2008-02-07 Univ Nihon Ph measuring instrument and ph measuring method

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20190005401A (en) 2017-07-06 2019-01-16 한림대학교 산학협력단 Biosensor for measuring impedance, temperature and hydrogen ion concentration
KR20190112935A (en) 2018-03-27 2019-10-08 재단법인 대구경북첨단의료산업진흥재단 pH DETECTING MICRO-STRUCTURED STICKER AND THE MANUFACTURING METHOD THEREOF

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