KR101160668B1 - A biochip including a light emitting device and a bio diagnosis device and method using thereof - Google Patents

A biochip including a light emitting device and a bio diagnosis device and method using thereof Download PDF

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KR101160668B1
KR101160668B1 KR1020100063662A KR20100063662A KR101160668B1 KR 101160668 B1 KR101160668 B1 KR 101160668B1 KR 1020100063662 A KR1020100063662 A KR 1020100063662A KR 20100063662 A KR20100063662 A KR 20100063662A KR 101160668 B1 KR101160668 B1 KR 101160668B1
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light emitting
emitting device
biochip
bio
light
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KR1020100063662A
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KR20120003043A (en
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이도영
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(주)실리콘화일
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Abstract

The present invention relates to a biochip, and more particularly, a biolayer in which a biochemical reaction between a reference sample and a target sample occurs and an image sensor layer sensing light emitted from the biolayer are combined into a single chip. It relates to a biochip with a light emitting device.
The present invention has an advantage that the incident light of the light emitting device does not enter the image sensor layer without a separate filter layer by embedding the light emitting device inside the biochip and placing a reflecting plate under the light emitting device.

Description

Biochip including a light emitting device, a bio diagnostic device and method using the biochip {A biochip including a light emitting device and a bio diagnosis device and method using

The present invention relates to a biochip, a biodiagnostic apparatus and a method, and more particularly, to a biochip, a biodiagnostic apparatus and a method incorporating a light emitting device.

In general, a biochip is formed in a form in which a reference sample composed of biological materials such as DNA and protein is regularly arranged on a substrate made of a material such as glass, silicon, metal such as gold, or nylon.

Biochip basically uses the biochemical reaction between the reference sample and the target sample immobilized on the substrate. Representative examples of such biochemical reactions between the reference sample and the target sample include complementary binding between DNA bases and antigen-antibody reactions. .

Diagnosis by biochip is mostly done by detecting the extent to which the biochemical reactions of the reference sample and the target sample occur through optical processes. Generally used optical processes utilize fluorescence or luminescence.

1 is a cross-sectional view of a conventional biochip combined with an image sensor.

Referring to FIG. 1, the biochip 100 to which the conventional image sensor is coupled includes a bio layer 110, a filter layer 120, and an image sensor layer 130.

The biolayer 110 is where the biochemical reaction between the reference sample and the target sample occurs. As a result of the biochemical reaction, the light emitting material or the fluorescent material remains.

In this case, when the light emitting material remains, light is generated from the light emitting material itself, so it is necessary to block external light. However, when the fluorescent material remains, a separate external light is required to emit the fluorescent material. Therefore, in this case, a filter layer is required to block the external light from being incident on the image sensor layer.

 The filter layer 120 is formed under the bio layer 110. The filter layer blocks the external light from entering the lower image sensor layer when fluorescent material remains as a result of the biochemical reaction.

That is, the external light corresponds to noise when determining whether the fluorescent material is fluorescence through the image sensor layer. Therefore, the filter layer serves to remove noise called external light.

The image sensor layer 130 is formed under the filter layer 120 and is composed of a plurality of photo detectors 131. Here, the plurality of light detectors 131 detects the light filtered by the filter layer and converts the light into an electrical signal.

However, such a conventional biochip 100 has a wavelength of external light (

Figure 112010042842690-pat00001
) And the wavelength of light from a luminescent or fluorescent material (
Figure 112010042842690-pat00002
There is a difficulty in designing an elaborate filter layer 120 that is very small and can filter out the small difference.

The technical problem to be solved by the present invention is to provide a biochip and a bio diagnostic apparatus using the same by forming a light emitting device for emitting a fluorescent material inside the biochip and placing a reflector under the light emitting device. have.

Another technical problem to be solved by the present invention is to provide a biochip and a bio diagnostic apparatus using the same so that light generated from a light emitting device or a fluorescent device does not enter the lower image sensor layer.

Another technical problem to be solved by the present invention is to provide a bio-diagnostic method that can properly determine the light generated from the light emitting device or the fluorescent device.

In the biochip including the light emitting device according to the present invention for achieving the above technical problem, a bio layer in which a plurality of reaction regions in which a biochemical reaction between a reference sample and a standard sample occurs is formed in the shape of a groove and a lower portion of the bio layer. And an image sensor layer having a plurality of photo detectors, wherein a light emitting device is included in the bio layer.

According to another aspect of the present invention, there is provided a biodiagnosis apparatus including a biochip having a light emitting device and a signal processor configured to analyze and process a signal output from the biochip.

According to another aspect of the present invention, there is provided a biodiagnostic method comprising: (a) storing first information generated by light emission of a light emitting device before a target sample is added to a reference sample; (b) storing second information generated by light emission of the light emitting device after the target sample is added to the reference sample; And (c) diagnosing the first information by comparing the second information with each other.

The present invention has the advantage that a separate filter layer is not required by forming a light emitting device for emitting a fluorescent material inside the biochip and placing a reflecting plate under the light emitting device.

1 is a cross-sectional view of a conventional biochip combined with an image sensor.
2 is a cross-sectional view of a biochip incorporating a light emitting device according to the present invention.
3 is a block diagram illustrating an embodiment of a bio diagnostic apparatus using a biochip incorporating a light emitting device according to the present invention.
Figure 4 is a block diagram showing another embodiment of a bio diagnostic apparatus using a biochip with a light emitting device according to the present invention.

Hereinafter, embodiments of the present invention will be described in detail with reference to the drawings.

2 is a cross-sectional view of a biochip incorporating a light emitting device according to the present invention.

Referring to FIG. 2, a biochip 200 having a light emitting device according to the present invention includes a bio layer 210 and an image sensor layer 220.

The bio layer 210 includes a plurality of reaction regions 211, a light emitting device 212, and a reflecting plate 213.

The plurality of reaction zones 211 are generally formed in the form of grooves on the upper bio layer 210 and are places where biochemical reactions occur between the reference sample 211a and the target sample 211b. The biochemical reaction is performed by adding the target sample 211b into the reaction region 211 provided with the reference sample 211a.

The reference sample 211a is a variety of samples for the biochemical reaction of the target sample 211b. The reference sample depends on which biochemical reactions are targeted in the biochip. For example, if the biochemical response is an antigen-antibody response, the reference sample may be an antigen.

The target sample 211b is determined according to the reference sample 211a. For example, if the reference sample is an antigen, the target sample may be blood or the like.

The light emitting device 212 is connected to a peripheral circuit (not shown) capable of emitting light of a predetermined wavelength λ 1 and controlling on-off and the like. In general, the light emitting device 212 emits light when a current flows and is preferably an LED (Light Emitting Diode) having excellent luminous efficiency.

The reflective plate 213 is formed inside the bio layer 210 and is positioned below the light emitting device 212. The reflective plate 213 serves as a blocking film so that light emitted from the light emitting device 212 is not incident on the image sensor layer 220. The reflector may be any material capable of reflecting light.

The reflective plate 213 may be appropriately modified in shape so that light emitted from the light emitting element 212 is not incident on the image sensor layer 220. For example, the reflective plate 213 may be configured to surround the light emitting device 212 and open only toward the fluorescent material remaining in the reaction region 211.

Although not shown, a filter layer may be added between the bio layer 210 and the image sensor layer 220 to block light emitted from the light emitting device, similarly to FIG. 1. The added filter layer prevents light emitted from the light emitting device 212 together with the reflector from entering the image sensor layer 220.

The image sensor layer 220 is formed under the bio layer 210 and includes a plurality of light detectors 221.

The plurality of light detectors 221 are formed on the surface of the image sensor layer 220 to sense light and generate a corresponding charge. Each of the plurality of photodetectors 221 is connected to a peripheral circuit (not shown) for generating a corresponding electrical signal based on the generated charges. For example, the plurality of light detectors 221 may include an image sensor in the form of a charge coupled device (CCD) or an image sensor in the form of a complementary MOS (CMOS).

3 is a block diagram illustrating an embodiment of a bio diagnostic apparatus using a biochip incorporating a light emitting device according to the present invention.

Referring to FIG. 3, the bio diagnostic apparatus 300 according to the present invention includes a biochip 310 and a signal processor 320 in which a light emitting device is embedded. Since the biochip 310 having the light emitting device is the same as described with reference to FIG. 3, it is omitted here.

The signal processor 320 analyzes an electrical signal output from the biochip after the biochemical reaction and outputs a diagnosis result. The signal processor 320 may be configured to have a built-in image signal processor (ISP), which is a program capable of analyzing electrical signals, in order to obtain a desired diagnosis result in a short time. In addition, if necessary, the signal processing unit 320 may be formed on the same substrate as the image sensor layer 220.

Figure 4 is a block diagram showing another embodiment of a bio diagnostic apparatus using a biochip with a light emitting device according to the present invention.

Referring to FIG. 4, the bio diagnostic apparatus 400 according to the present invention includes a biochip 410 having a light emitting device, a first storage 420, a second storage 430, a comparator 440, and The signal processor 450 is provided.

Since the biochip 410 having the light emitting device is the same as described with reference to FIG. 3, it is omitted here.

The first storage unit 420 stores the electrical signal output from the biochip by turning on the light emitting device 413 by a peripheral control circuit (not shown) before the biochemical reaction between the reference sample and the standard sample occurs. do.

The second storage unit 430 stores the electrical signal output from the biochip by turning on the light emitting device 413 by a peripheral control circuit (not shown) after the biochemical reaction between the reference sample and the standard sample. do.

In general, the first storage unit 420 and the second storage unit 430 preferably have a flash memory in which power consumption is small and the stored information does not disappear even when the power is turned off. In addition, the first storage unit 420 and the second storage unit 430 may configure one nonvolatile memory by dividing the regions.

The comparator 440 compares the electrical signal stored in the first storage 420 with the electrical signal stored in the second storage 430 and outputs the difference.

That is, the electrical signal stored in the first storage unit 420 is an electrical signal generated by light emitted only from the light emitting device before the biochemical reaction between the reference sample and the standard sample occurs.

In contrast, the electrical signal stored in the second storage unit 430 is an electrical signal generated by light emitted from not only a light emitting device but also a fluorescent material. This is because, after the biochemical reaction between the reference sample and the standard sample, fluorescent material is generated in the reaction zone of the biolayer.

Therefore, the difference between the electrical signals stored in the first storage unit 420 and the second storage unit 430 corresponds to the electrical signal generated by the light emitted from the fluorescent material remaining after the biochemical reaction.

The signal processor 450 analyzes the electrical signal output from the comparator after the biochemical reaction and outputs a diagnosis result. The signal processor 450 may have an operation function for obtaining a more organized signal, for example, a function of removing noise, even before or after storing the signals in the first storage unit 420 and the second storage unit 430. It may be included.

As described above with reference to FIG. 3, the signal processor may include an ISP (Image Signal Processor), which is a program capable of analyzing electrical signals, so that a desired diagnosis result may be obtained in a short time.

If necessary, the first storage unit 420, the second storage unit 430, the comparator 440, and the signal processor 450 may be formed on the same substrate as the image sensor layer 220.

Hereinafter, a biodiagnostic method using a biochip incorporating a light emitting device according to the present invention will be described.

According to an exemplary embodiment of the present invention, a biodiagnostic method stores first information generated by light emission of a light emitting device embedded in a biochip before a target sample is added to the reference sample, and after the target sample is added to the reference sample, Storing second information generated by light emission of an embedded light emitting device, and diagnosing the first information by comparing the second information with each other.

Preferably, the storing of the first information and the second information is performed after detecting the light emission from the light emitting element by a plurality of light detectors, wherein the first information and the second information are stored in the plurality of information. It is an electrical signal from a light sensor.

The diagnosing step is preferably performed by a circuit formed on the semiconductor substrate. If necessary, the diagnostic result can be obtained in advance of a signal processing step including an operation function for obtaining more organized information, for example, a function for removing noise.

While the present invention has been described in connection with what is presently considered to be the most practical and preferred embodiment, it is to be understood that the invention is not limited to the disclosed embodiments. It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the scope of the present invention.

Claims (10)

In the biochip,
Bio layer having a plurality of reaction zones in the form of grooves in which the biochemical reaction between the reference sample and the standard sample occurs;
Is formed below the bio layer, and includes an image sensor layer is formed a plurality of photo detectors (Photo Detector),
A biochip with a light emitting device, characterized in that the light emitting device is included in the bio layer. The method of claim 1, wherein the bio layer is inside
And a reflecting plate formed under the light emitting device and reflecting light emitted from the light emitting device. The method of claim 2, wherein the light emitting device
Biochip with a light emitting device, characterized in that the LED (Light Emitting Diode). The method of claim 3, wherein the plurality of light detectors
Biochip with a light emitting device, characterized in that the CCD (Charge Coupled Device) or CMOS (Complementary MOS) image sensor. The method of claim 4, wherein
And a filter layer positioned between the bio layer and the image sensor layer to block light emitted from the light emitting device. In the bio diagnostic apparatus,
A biochip incorporating the light emitting element according to any one of claims 1 to 5
And a signal processor for analyzing and processing the signal output from the biochip. In the bio diagnostic apparatus,
A biochip containing the light emitting device according to any one of claims 1 to 5;
A first storage unit storing a signal output from the biochip before a biochemical reaction between the reference sample and the standard sample occurs;
A second storage unit storing a signal output from the biochip after the biochemical reaction occurs;
A comparison unit comparing the signal stored in the first storage unit with the signal stored in the second storage unit; And
And a signal processor configured to analyze and process the signal output from the comparator. In the bio diagnostics method,
(a) storing first information generated by light emission of the light emitting device before the target sample is added to the reference sample;
(b) storing second information generated by light emission of the light emitting device after the target sample is added to the reference sample; And
and (c) diagnosing the first information by comparing the second information with each other. The method of claim 8, wherein step (a) and step (b)
The bio-diagnostic method according to claim 1, wherein the bio-diagnosis method is performed after light emission from the light emitting device is detected by a plurality of light sensors. The method of claim 9, wherein step (c)
A biodiagnosis method, characterized in that it is carried out by a circuit formed on a semiconductor substrate.
KR1020100063662A 2010-07-02 2010-07-02 A biochip including a light emitting device and a bio diagnosis device and method using thereof KR101160668B1 (en)

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KR101569515B1 (en) 2014-08-13 2015-11-27 (주)옵토레인 A biochip including a side- light type light emitting device and a manufacturing method thereof
KR101727301B1 (en) * 2014-09-25 2017-04-14 (주)옵토레인 A manufacturing method for biochip improved sensitivity property for fluorescence signal and the biochip manufactured by the same
EP3001182B1 (en) * 2014-09-25 2017-11-29 Optolane Technologies Inc. Method for manufacturing biochip having improved fluorescent signal sensing properties and biochip manufactured by the same
KR102625129B1 (en) * 2018-08-23 2024-01-15 엘지디스플레이 주식회사 Wearable photoluminescence sensor and remote sensing apparatus including the same

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US20050063870A1 (en) * 2003-09-01 2005-03-24 Seiko Epson Corporation Biosensor and method of manufacturing biosensor
KR100801448B1 (en) * 2007-05-16 2008-02-11 (주)실리콘화일 Bio chip
KR20080098855A (en) * 2007-05-07 2008-11-12 삼성전자주식회사 Filterless biosensor using multiple oled light sources
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Publication number Priority date Publication date Assignee Title
US20050063870A1 (en) * 2003-09-01 2005-03-24 Seiko Epson Corporation Biosensor and method of manufacturing biosensor
KR20080098855A (en) * 2007-05-07 2008-11-12 삼성전자주식회사 Filterless biosensor using multiple oled light sources
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KR100923461B1 (en) * 2007-10-31 2009-10-27 전자부품연구원 A sample analysis appparatus for a micro-plate

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