KR101119445B1 - Making process of a flatfish feed using curcuma longa and alginic acid - Google Patents
Making process of a flatfish feed using curcuma longa and alginic acid Download PDFInfo
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- KR101119445B1 KR101119445B1 KR1020090099491A KR20090099491A KR101119445B1 KR 101119445 B1 KR101119445 B1 KR 101119445B1 KR 1020090099491 A KR1020090099491 A KR 1020090099491A KR 20090099491 A KR20090099491 A KR 20090099491A KR 101119445 B1 KR101119445 B1 KR 101119445B1
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- A—HUMAN NECESSITIES
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- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
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- A23K20/163—Sugars; Polysaccharides
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Abstract
본 발명은 정수에 알긴산농도가 1~2%가 되도록 알긴산을 첨가하여 90~150℃서 20~30분 가열 용해하고, 상기 알긴산 용해액에 울금의 농도가 1.25~3.75%가 되도록 울금 분말을 첨가하여 90~150℃에서 5~10분 가열 용해한 다음, 상기 알긴산과 울금 용해액을 넙치사료에 분무하여 코팅하고, 60℃ 항온 열풍건조기에서 15~30분 동안 건조하여 제조되는 알긴산과 울금을 이용한 넙치사료 제조방법을 제공하기 위한 것으로, 이처럼 본 발명은 알긴산과 울금을 농도별로 넙치사료에 코팅함으로써 넙치 증체율 개선 효과뿐만 아니라 친환경 양식기반을 구축할 수 있으며, 넙치 양식업자의 소득증진에 크게 기여할 수 있는 매우 유용한 발명인 것이다.The present invention adds alginic acid to the purified water so that the alginic acid concentration is 1 to 2%, and is dissolved by heating for 20 to 30 minutes at 90 to 150 ° C., and turmeric powder is added to the alginic acid solution so that the concentration of turmeric is 1.25 to 3.75%. And heat dissolved at 90-150 ° C. for 5-10 minutes, and then spray the alginic acid and turmeric solution on the flounder feed, and dry it for 15-30 minutes in a 60 ° C. constant temperature hot air dryer. In order to provide a method of manufacturing a feed, the present invention can be coated with the alginate and turmeric on the flounder feed by concentration to build an eco-friendly farming as well as to improve the flounder growth rate, which can greatly contribute to the income of the flounder aquaculture industry It is a very useful invention.
알긴산, 울금, 넙치사료, 제조방법 Alginic acid, turmeric, olive flounder, manufacturing method
Description
본 발명은 알긴산과 울금을 이용한 넙치사료 제조방법에 관한 것으로, 더욱 상세하게는 식용 적법성이 판정된 공시 재료인 알긴산과 울금을 이용하여 넙치사료를 제조하고 사용시 넙치 치어의 증체율 개선뿐만 아니라 친환경 양식기반 구축 및 산업화에 크게 기여할 수 있어 해산물 생산, 가공 및 시판업자의 소득증진과 경제적 부가가치 창출을 가능케 하는 알긴산과 울금을 이용한 넙치사료 제조방법에 관한 것이다.The present invention relates to a method for producing halibut feed using alginic acid and turmeric, and more particularly, to prepare a halibut feed using alginic acid and turmeric, which have been determined to be edible, and to improve not only the weight gain of the flounder fry, but also an eco-friendly farming base. The present invention relates to a method of manufacturing halibut feed using alginic acid and turmeric, which can greatly contribute to the construction and industrialization, which enables income production and economic added value of seafood production, processing, and marketing.
최근 전 세계적으로 식품의 안전성에 대한 소비자의 의식이 높아지고 있고, 농축수산의 모든 분야에서 화학합성 약품에 대한 규제가 강화됨에 따라 차세대 양식산업을 선도하기 위한 친환경 넙치사료의 개발이 절실히 요구되고 있다.Recently, consumer awareness of food safety is increasing all over the world, and as regulations on chemical synthetic drugs are strengthened in all areas of concentrated fisheries, development of eco-friendly halibut feed to lead the next generation aquaculture industry is urgently required.
특히 양식 어류의 사료효율성 및 양어장 내 수질 및 어류의 성장 속도 개선에 적합한 친환경 넙치사료의 개발이 절실히 요구되고 있는 추세이다. 현재 국내 양식장에서 사용되고 있는 넙치사료(MP)의 비중은 약 80%를 차지하고 있으며, 이러한 넙치사료(MP)의 공급시 발생하는 사료의 수중 유실은 양식장 배출수 오염 문제로 인한 연안 환경오염 유발, 양식장 수조의 오염으로 인한 어류질병의 빈번한 발생현황, 생사료의 손실 등의 문제점에 기인하여 친환경 양식 산업을 위한 어류양식용 사료의 코팅기술 개발은 필수불가결하다.In particular, the development of eco-friendly halibut feed suitable for improving feed efficiency of farmed fish, water quality in fish farms, and growth rate of fish is urgently required. Currently, the percentage of halibut feed (MP) used in domestic farms is about 80%, and the loss of feed water caused by the supply of halibut feed (MP) causes coastal environmental pollution due to contamination of farmwater discharges, and fish tanks. Due to problems such as frequent occurrence of fish disease and loss of live feed due to pollution of fish, development of coating technology of fish farming feed for green farming industry is indispensable.
그리고 현재 정부 양식장 사료로 인한 환경개선 방안으로서 2010년까지 넙치사료(EP)를 개발하여 환경오염 방지를 위해 노력하고 있는 추세이다.In addition, as a plan to improve the environment caused by the government's aquaculture feed, the halibut feed (EP) has been developed until 2010 to prevent environmental pollution.
그 일환으로 최근 한약재나 미생물을 이용하여 증체율 개선 및 항생제를 대체하고자 하는 연구가 다양하게 추진되고 있으나, 한약재는 원료의 특성에 따른 적정한 추출법의 확립 등 과학적인 접근이 아직 이루어 지지 않고 있는 실정이다.As part of this research, various studies have been conducted to improve the weight gain rate and replace antibiotics using herbal medicines or microorganisms. However, the scientific approach has not yet been made such as establishment of proper extraction method according to the characteristics of raw materials.
따라서 해양 수산자원 및 천연물로부터 천연물 화학 기법을 토대로 증체율 개선 물질을 생산하여 양식 어류의 증식효율 개선뿐만 아니라 기존의 화학합성제를 대체할 수 있는 친환경 기능성 넙치사료의 개발에 의한 친환경 양식기반 조성이 절실하다 할 것이다.Therefore, it is necessary to produce eco-friendly aquaculture based on the development of eco-functional halibut feed, which can replace the existing chemical synthetic materials as well as improve the growth efficiency of aquaculture fish by producing materials to increase the rate of growth based on natural chemical techniques from marine aquatic resources and natural products. Will do.
이에 본 발명자는 넙치 증체율 개선을 위한 넙치사료를 개발하기 위해 수없이 많은 연구와 노력 끝에, 정수(淨水)에 알긴산농도가 1~2%가 되도록 알긴산을 첨가하여 90~150℃서 20~30분 가열 용해하고, 상기 알긴산 용해액에 울금의 농도가 1.25~3.75%가 되도록 울금 분말을 첨가하여 90~150℃에서 5~10분 가열 용해한 다음, 상기 알긴산과 울금 용해액을 넙치사료에 분무하여 코팅한 후, 60℃ 항온 열풍건조기에서 15~30분 동안 건조하여 제조되는 알긴산과 울금을 이용한 넙치사료를 개발하게 된 것이다.Therefore, the present inventors endeavored to develop the flounder feed for improving the flounder growth rate, and added alginic acid so that the alginate concentration was 1 ~ 2% in purified water. The mixture was heated and dissolved in minutes, and the turmeric powder was added to the alginate solution so that the concentration of turmeric is 1.25 to 3.75%. The solution was heated and dissolved at 90 to 150 ° C. for 5 to 10 minutes, and then the alginic acid and turmeric solution were sprayed onto the flounder feed After coating, it was to develop the halibut feed using alginic acid and turmeric produced by drying for 15-30 minutes in 60 ℃ constant temperature hot air dryer.
이처럼 본 발명은 알긴산과 울금을 농도별로 넙치사료에 코팅함으로써 넙치 증체율 개선 효과뿐만 아니라 친환경 양식기반을 구축할 수 있으며, 넙치 양식업자의 소득증진에 크게 기여할 수 있는 매우 유용한 발명인 것이다.As described above, the present invention is not only to improve the flounder growth rate but also to build an eco-friendly farming base by coating alginic acid and turmeric on the flounder feed by concentration, and is a very useful invention that can greatly contribute to the increase of income of the flounder farmer.
상기한 효과를 달성하기 위한 본 발명에 따른 알긴산과 울금을 이용한 넙치사료 제조방법은, 정수(淨水)에 알긴산농도가 1~2%가 되도록 알긴산을 첨가하여 90~150℃서 20~30분 가열 용해하고, 상기 알긴산 용해액에 울금의 농도가 1.25~3.75%가 되도록 울금 분말을 첨가하여 90~150℃에서 5~10분 가열 용해한 다음, 상기 알긴산과 울금 용해액을 넙치사료에 분무하여 코팅하고, 60℃ 항온 열풍건조기에서 15~30분 동안 건조하여 제조된다.Flounder feed manufacturing method using alginic acid and turmeric in accordance with the present invention for achieving the above effect, by adding alginic acid so that the concentration of alginic acid to 1 ~ 2% in purified water (20 ~ 30 minutes at 90 ~ 150 ℃) Heat dissolve, add turmeric powder to the alginate solution so that the concentration of turmeric is 1.25 ~ 3.75%, heat dissolve at 90 ~ 150 ° C. for 5-10 minutes, and then spray the alginic acid and turmeric solution on halibut feed And, it is prepared by drying for 15-30 minutes in a 60 ℃ constant temperature hot air dryer.
이하 바람직한 실시예를 통해서 본 발명을 상세히 설명하면 다음과 같다.Hereinafter, the present invention will be described in detail with reference to the following preferred embodiments.
[실시예][Example]
먼저 정수 1ℓ에 알긴산이 1%농도를 가지도록 첨가하여 90~150℃에서 20~30분 가열 용해한다.First, alginic acid is added to 1 liter of water to have a concentration of 1%, and dissolved by heating at 90 to 150 ° C. for 20 to 30 minutes.
다음 상기 알긴산 용해액에 울금 분말이 각각 1.25%, 2.5%, 3.75%농도를 가지도록 첨가하여 90~150℃에서 5~10분 가열 용해한다.Next, the turmeric powder is added to the alginic acid solution so as to have a concentration of 1.25%, 2.5%, and 3.75%, respectively, and dissolved by heating at 90 to 150 ° C. for 5 to 10 minutes.
다음 상기 알긴산과 울금 분말의 용해액을 넙치사료, 즉 넙치 공시 치어의 사료로서 넙치 2호에 해당하는 EP사료(수협사료, 입자도 2.0~2.2㎜, 대상어 체장 9~15㎝, 체중 10g 중량)에 0.125%, 0.25%, 0.375%농도를 가지도록 분무하여 코팅한 후, 60℃ 항온 열풍건조기에서 15~30분 동안 건조하여 본 발명 넙치사료를 각각 제조한다.Next, the solution of the alginic acid and turmeric powder is the flounder feed, that is, the EP feed corresponding to the flounder No. 2 as the feed of the flounder published fry (water stalk feed, particle size 2.0-2.2㎜, target fish body length 9-15cm, weight 10g weight) After spraying and coating so as to have a concentration of 0.125%, 0.25%, 0.375%, and dried for 15-30 minutes in a 60 ℃ constant temperature hot air dryer to prepare the halibut feed of the present invention, respectively.
상기와 같이 각각 0.125%, 0.25%, 0.375%농도로 제조된 넙치사료를 넙치 치어(n=30,10-11㎝)에 1일 3회 급여하였다. 이때 넙치 치어의 어항 크기는 가로:세로:높이 = 750*350*500㎜로써 18~20℃의 냉장 조건을 유지할 수 있도록 설비한 실험조건에서 산소공급을 위하여 Aquarium Filter(주, 협신, Model-1500) 및 자동히터 AH-150(주, 삼호)을 구비하여 실시하였다. 급여 4주경과 후 대조군(동일 조건에서 넙치 2호에 해당하는 EP사료를 [표 1]의 권장 급여율 표에 준하여 넙치에 1일 3회 급여)과 0.375%농도로 코팅된 처리군은 아래의 [표 2]와 [그림 1]에서 확인되는 바와 같이 유의적 차이가 있었으며, 넙치 치어의 증체율에 효과(P<0.05)가 있음을 나타내었다.Halibut feed prepared in 0.125%, 0.25%, 0.375% concentration as described above was fed to flounder fry (n = 30,10-11cm) three times a day. At this time, the size of fish tank of halibut fry is horizontal: length: height = 750 * 350 * 500mm, and Aquarium Filter (Note, Hsinshin, Model-1500) for oxygen supply under experimental conditions equipped to maintain refrigeration conditions of 18 ~ 20 ℃. ) And automatic heater AH-150 (Note, Samho). After 4 weeks of salary, the control group (EP feed corresponding to flounder No. 2 under the same conditions, 3 times a day on the flounder according to the recommended salary ratio table in Table 1) and the treatment group coated with 0.375% concentration were As shown in [Table 2] and [Figure 1], there was a significant difference, indicating that there was an effect (P <0.05) on the growth rate of flounder fry.
[표 1] 권장 급여율[Table 1] Recommended Salary Rate
[표 2] 넙치 치어의 체중변화[Table 2] Weight change of flounder fry
[그림 1] 넙치 치어의 체중변화[Figure 1] Changes in weight of flounder fry
본 발명의 기능성 원료물질인 울금, 알긴산을 이용하여 넙치사료의 제조과정 동안 중금속의 오염 또는 잔류 가능성을 입증하기 위해 유해물질 분석을 2007년 6월 28일 식품의약품안전청고시 제2007-51호에서 제시한 "건강기능식품 기능성 원료인정에 관한 규정"에 근거하여 식품공전에 준하여 실시하였다.To prove the possibility of contamination or residual heavy metals during the manufacturing process of halibut feed using the functional raw materials of turmeric and alginic acid of the present invention, the analysis of harmful substances is presented in the Food and Drug Administration Notification No. 2007-51 on June 28, 2007. It was conducted in accordance with the Food Code, based on the "Regulations on Functional Ingredients Recognition of Functional Health Foods".
[표 3] 사료 첨가제의 중금속 분석Table 3 Analysis of Heavy Metals in Feed Additives
상기한 바와 같이, 중금속을 분석한 결과는 3회 반복실험에 의한 것으로 납, 카드뮴, 수은은 검출되지 않았다.As described above, the result of analyzing the heavy metals was three times, and lead, cadmium, and mercury were not detected.
2007년 06월 28일 식품의약품안전청고시 제2007-51호에서 제시한 “건강기능식품 기능성 원료인정에 관한 규정”에 근거하여 BHC, DDT, 엔드린, 디엘드린, 알드린 및 기타 농약성분에 대한 분석을 식품공전에 준하여 실시한 다음 3회 반복 평균값으로 환산하였다.In accordance with the Regulation on the Recognition of Functional Ingredients for Functional Functional Food, as set forth in the Food and Drug Administration Notification No. 2007-51 on June 28, 2007, the BHC, DDT, Endrin, Dieldrin, Aldrin and other pesticide components The analysis was carried out according to the Food Code, and then converted into three replicate average values.
[표 4] 사료 첨가제의 잔류 농약분석Table 4 Residual Pesticide Analysis of Feed Additives
식품공전에 준하여 울금 혼합물에 대한 23종의 잔류농약을 분석한 결과 불 검출로 판정되었다. BHC, DDT, 엔드린, 디엘드린, 알드린 및 기타 농약성분에 대한 분석을 식품공전에 준하여 실시한 다음 3회 반복하여 통계적 신뢰성을 입증하였다.According to the Food Code, 23 residues of pesticides were analyzed for turmeric mixture. Analyzes of BHC, DDT, endrin, dieldrin, aldrin and other pesticides were performed according to the Food Code and then repeated three times to demonstrate statistical reliability.
2007년 06월 28일 식품의약품안전청고시 제2007-51호에서 제시한 “건강기능식품 기능성 원료인정에 관한 규정”에 근거하여 총균수, 병원성 식중독균 (대장균, E. coli O157:H7, Salmonella spp., Staphylococcus aureus, Listeria monocytogenes, Vibrio spp., Clostridium perfringens 등) 및 곰팡이에 대한 분석을 식품공전에 준하여 실시한 다음 3회 반복 평균값으로 환산하였다. 넙치 치어 사료 첨가제의 유해물질 시험방법을 식품공전에 준하여 실시한 3회 반복 실험 결과 모두 총 세균수, 곰팡이 및 병원성 세균이 음성으로 분석되었다.Based on the “Regulations on Functional Ingredients Recognition of Functional Functional Foods” presented in the Food and Drug Administration Notification No. 2007-51 of June 28, 2007, the total number of bacteria and pathogenic food poisoning bacteria (E. coli O157: H7, Salmonella spp. , Staphylococcus aureus, Listeria monocytogenes, Vibrio spp., Clostridium perfringens, etc.) and fungi were analyzed according to the Korean Food Code and then converted into three replicate average values. The total number of bacteria, fungi and pathogenic bacteria were negatively analyzed in the results of three repeated experiments of Halibut Poison Feed Additives according to the Food Code.
[표 5] 사료 첨가제의 총 세균수, 곰팡이 및 병원성 미생물의 분석Table 5 Analysis of total bacterial counts, fungi and pathogenic microorganisms of feed additives
상기한 바와 같이, 본 발명 넙치사료는 사료 첨가제로서 사용가능함을 입증하였다.As mentioned above, the halibut feed of the present invention has proved usable as a feed additive.
본 발명 넙치사료의 일반성분은 AOAC(27) 방법에 따라 분석하였다. 즉 수분 은 105℃ 건조법, 조회분은 550℃ 직접회화법, 조단백은 micro-kjeldahl법, 조지방은 Soxhlet 추출법에 의하여 분석하였다.General components of the halibut feed of the present invention were analyzed according to the AOAC (27) method. Moisture was analyzed by drying at 105 ℃, crude ash at 550 ℃, direct protein by micro-kjeldahl, and crude fat by Soxhlet extraction.
상기한 바와 같이 분석한 결과, 본 발명 넙치사료는 조단백질은 52%, 조지방질은 12%, 조회분은 13% 그리고 수분은 13%로 나타났다.As a result of the analysis as described above, the crude halibut feed of the present invention was found to be 52% crude protein, 12% crude fat, 13% crude ash and 13% moisture.
본 발명 넙치사료 1g을 550℃로 12시간 회화하고 건식분해법으로 분해하여 deionized 증류수로 정용하여 검액으로 하였다. 즉, 마쇄한 시료 1g에 진한 질산 10㎖를 가하여 처음에는 낮은 온도로 가열하고 점차 고온으로 가열하면서 분해하였다. 분해액이 백색 투명하게 되면 냉각시키고 분해액에 증류수를 가하고 50㎖로 정용 후 여과하여 여액을 분석 시료로 하였다. 각 무기성분의 정량은 유도 결합플라스마 분광광도기(이하ICP) 이용하여 분석하였으며 분석 조건은 아래의 [표 6]과 같다.1 g of the halibut feed of the present invention was incubated at 550 ° C. for 12 hours, decomposed by dry decomposition, and then purified by deionized distilled water to obtain a sample solution. That is, 10 ml of concentrated nitric acid was added to 1 g of the ground sample, which was initially decomposed while heating to a low temperature and gradually heating to a high temperature. When the decomposition solution became white transparent, it was cooled, distilled water was added to the decomposition solution, and after filtrating with 50 ml, the filtrate was used as an analytical sample. Quantification of each inorganic component was analyzed using an inductively coupled plasma spectrophotometer (ICP) and the analysis conditions are shown in Table 6 below.
[표 6] 넙치사료의 무기질 ICP 분석 결과[Table 6] Results of Mineral ICP Analysis of Olive Flounder
ICP 방법에 의해 넙치 치어의 넙치사료에 대한 무기질을 분석한 결과, 칼슘(Ca)이 가장 많이 함유되어 있었으며, 그 다음으로 P, Na, Mg 순으로 함유되어 있었다.As a result of analyzing the minerals of the flounder fry by ICP method, calcium (Ca) was the highest, followed by P, Na, and Mg.
본 발명 넙치사료를 AOAC 방법에 준하여 유리당을 분석하였다. 즉, 시료 10 g를 증류수로 100㎖ 정용하여 30℃ 수욕조에서 30분 동안 진탕한 후 3,000rpm에서 30분간 원심분리하였다. 원심분리한 상등액을 취하여 15,000rpm으로 30분간 원심분리하였다. 상등액을 Sepak-C18을 사용하여 정제시킨 후 membrane filter(0.45㎛로 여과한 여액 20㎕를 HPLC에 주입하여 유리당을 분석하였으며 외부표준법으로 계산하였다.The free flounder diet of the present invention was analyzed according to the AOAC method. That is, 10 g of the sample was applied to 100 ml of distilled water, shaken in a 30 ° C. water bath for 30 minutes, and centrifuged at 3,000 rpm for 30 minutes. The supernatant was centrifuged and centrifuged at 15,000 rpm for 30 minutes. After the supernatant was purified using Sepak-C18, 20 μl of a membrane filter (0.45 μm filtered filtrate) was injected into HPLC to analyze free sugar and calculated by external standard method.
[표 7] 넙치사료의 유리당 HPLC 분석 결과Table 7 Free Sugar HPLC Analysis of Olive Flounder Feed
[그림 2] 넙치사료의 유리당 HPLC 크로마토그람[Figure 2] HPLC chromatogram of free sugar of halibut feed
HPLC방법에 의해 넙치 치어의 넙치사료에 대한 유리당을 분석한 결과는 포도당이 가장 많이 함유되어 있었으며 그 다음이 과당이었다.As a result of the analysis of free sugars on the flounder feed of the flounder fry by HPLC method, glucose contained the most, followed by fructose.
본 발명 넙치사료의 지방산을 분석하기 위해서는 아래의 방법을 이용하였다.In order to analyze the fatty acid of the halibut feed of the present invention, the following method was used.
지질성분 추출 및 정제Lipid Component Extraction and Purification
건조시료 5g씩을 chloroform:MeOH:water = 2:1:1(v/v)의 용매계로 Bligh와 Dyer의 방법에 따라 총지질을 추출하였다.5 g each of the dry samples was extracted with total chloroform: MeOH: water = 2: 1: 1 (v / v) solvent according to the method of Bligh and Dyer.
즉, 시료에 대하여 20배의 용매(100 ㎖)를 넣어 homogenizer(Nissei사, Ace homogenizer AM-11, Japan)로 파쇄하면서 섞고, 분액여두에 옮겨 때때로 흔들고 저어 주면서 24시간 동안 정치한 후, chloroform층만 분리하고 잔사에 다시 상기용매를 넣고 같은 조작을 되풀이하여 만 12시간 방치 후, chloroform층을 다시 분리하는 조작을 전 3회 반복 실시하였다.In other words, add 20 times the solvent (100 ml) to the sample, crush it with a homogenizer (Nissei, Ace homogenizer AM-11, Japan), mix, transfer to an aliquot, sometimes shake and stir, and then stand still for 24 hours. After separation, the solvent was added to the residue again, and the same operation was repeated. After standing for 12 hours, the chloroform layer was separated again.
Chloroform층을 감압농축하여 용매를 제거하고 진공 oven을 사용하여 감압하에서 24시간 건조 후, 질소가스를 충전하여 냉동보관하면서 다음 분석에 이용하였다.The chloroform layer was concentrated under reduced pressure to remove the solvent, dried under reduced pressure using a vacuum oven for 24 hours, and then charged with nitrogen gas and used for the next analysis while freezing.
지질 시료를 ethylether에 녹여 10 ㎖로 정용한 다음 그중 2㎖를 취하여 질소가스로 완전 농축하고 0.5N NaOH in MeOH 용액 8㎖를 가하여 100℃의 수조에서 완전히 용해될 때까지 가열(약 5분)한 후, 5 ㎖의 14% BF3-MeOH 용액을 가하고 2분간 비등시켰다.The lipid sample was dissolved in ethylether and applied to 10 ml. After that, 2 ml of it was completely concentrated with nitrogen gas, and 8 ml of 0.5 N NaOH in MeOH solution was added and heated until it completely dissolved in a water bath at 100 ° C. (about 5 minutes). 5 ml of 14% BF3-MeOH solution was added and boiled for 2 minutes.
충분히 포화시킨 NaCl 용액 20 ㎖를 flask에 가한 후, 전체 혼합물을 분액여두에 옮긴 다음, 20 ㎖의 petroleum ehter(b.p 30~60℃)를 가하여 1분간 강하게 흔들고 방치한 후, 층이 갈라지면 수용액 부분은 버리고 ether층만을 50㎖의 비이커에서 여과지(whatman No. 2)로 여과하여 취하고, 질소가스로 농축하였다.20 ml of sufficiently saturated NaCl solution is added to the flask, and then the whole mixture is transferred to the separating funnel. Then, 20 ml of petroleum ehter (bp 30 to 60 ° C.) is added, and the mixture is shaken and left for 1 minute. Was discarded, and only the ether layer was collected by filtration through a filter paper (whatman No. 2) in a 50 ml beaker and concentrated with nitrogen gas.
농축액을 petroleum ehter에 녹여 전량 1㎖로 하여 (2배 농축) GC 분석을 실시하였다. 지방산 표품(500 ppm 농도)에 대하여 동일조작을 실시한 다음 정성 및 정량을 행하였다.The concentrate was dissolved in petroleum ehter and subjected to GC analysis with a total amount of 1 ml (double concentration). The same procedure was carried out on the fatty acid standard (500 ppm concentration), followed by qualitative and quantitative analysis.
[표 8] 넙치사료의 지방산 GC 분석 결과Table 8 Results of Fatty Acid GC Analysis of Olive Flounder
[그림 3] EP 사료의 지방산 GC/MS 크로마토그람[Figure 3] Fatty acid GC / MS chromatogram of EP feed
[그림 4] EP 사료의 지방산 GC Chromatogram[Figure 4] Fatty acid GC Chromatogram of EP feed
상기한 바와 같이, 본 발명에서 제조한 넙치사료에 대해 지질을 추출 및 용매분획을 통하여 정제 후, GC에 의해 지방산을 분석한 결과 포화지방산 중에서는 팔미틴산이 가장 많은 함량을 나타내었다. 불포화지방산 중에서는 올레산이 가장 많은 함량을 나타내었고, 필수지방산인 리놀산, 리놀렌산, 아라키돈산의 함량도 13% 이상 함유되어 있어 영양학적 조성이 우수하였다. 일반적으로 불포화 지방산은 생물체 성장에 효과적인 것으로 알려져 있으며, 치어의 증체율 개선에 효과를 보인 것으로 검토되었다.As described above, after the lipid was extracted and purified through a solvent fraction of the olive flounder feed prepared in the present invention, fatty acid was analyzed by GC, and palmitic acid showed the highest content among saturated fatty acids. Among unsaturated fatty acids, oleic acid showed the highest content, and essential fatty acids such as linoleic acid, linolenic acid, and arachidonic acid contained more than 13%, which was excellent in nutritional composition. In general, unsaturated fatty acids are known to be effective for the growth of living organisms, and have been considered to be effective in improving the growth rate of fry.
이처럼 본 발명 넙치사료는, 즉 넙치 치어 사료(수협 2S호 사료)에 1.0~2.0%농도의 알긴산과 1.25~3.75%농도의 울금 분말을 가열 용해한 다음 상기 알긴산과 울금 분말의 용해액을 넙치사료에 분무하여 코팅한 후 건조하여 제조됨으로써 넙치 치어의 증체율 개선효과를 가능하게 하고, 친환경 양식기반 구축에 크게 기여할 수 있을 것이다.Thus, the present invention of the halibut feed, that is, to melt the 1.0-2.0% concentration of alginic acid and 1.25-3.75% concentration of turmeric powder in the flounder fry feed (suhyup 2S No. feed), and then the solution of the alginic acid and turmeric powder to the halibut feed It is possible to improve the rate of increase of flounder fry and contribute to the establishment of environment-friendly aquaculture base by spraying, coating and drying.
또한 본 발명 알긴산과 울금 분말을 이용하여 제조된 넙치사료의 개발과 산업화는 양식어민의 경제적 부가가치 창출 및 상품성 향상을 위한 유용한 방안이 될 수 있을 것이다.In addition, the development and industrialization of the halibut feed prepared using the alginic acid and turmeric powder of the present invention may be a useful way to create economic value added and commercialization of fishermen.
상기한 바와 같이 본 발명은 비록 한정된 실시예에 의해 설명되었으나, 본 발명은 이것에 한정되지 않으며 본 발명이 속하는 기술분야에서 통상의 지식을 가진 자에 의해 본 발명의 기술사상과 아래에 기재될 특허청구범위의 균등범위 내에서 다양한 수정 및 변형이 가능하다 할 것이다.As described above, the present invention has been described by means of a limited embodiment, but the present invention is not limited thereto, and the technical spirit of the present invention and a patent will be described below by those skilled in the art to which the present invention pertains. Various modifications and variations will be possible within the scope of the claims.
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