KR100874875B1 - 이식용 스트로 내 단위 구획별 수정란 봉입방법과 이식방법 - Google Patents
이식용 스트로 내 단위 구획별 수정란 봉입방법과 이식방법 Download PDFInfo
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- KR100874875B1 KR100874875B1 KR1020080046663A KR20080046663A KR100874875B1 KR 100874875 B1 KR100874875 B1 KR 100874875B1 KR 1020080046663 A KR1020080046663 A KR 1020080046663A KR 20080046663 A KR20080046663 A KR 20080046663A KR 100874875 B1 KR100874875 B1 KR 100874875B1
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D19/00—Instruments or methods for reproduction or fertilisation
- A61D19/04—Instruments or methods for reproduction or fertilisation for embryo transplantation
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Veterinary Medicine (AREA)
- Environmental Sciences (AREA)
- Animal Husbandry (AREA)
- Transplantation (AREA)
- Reproductive Health (AREA)
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Abstract
Description
구 분 | 이송거리 | ||
100km 이하 | 100~200km | 200km 이상 | |
종래방법(세워서 이송) | 4/20(20%) | 7/20(35%) | 10/20(50%) |
본 발명방법(눕혀서 이송) | 0/20(0%) | 0/20(0%) | 0/20(0%) |
구분 | 임신율 (%) | 유산발생율 (%) | 쌍태분만율 (%) | 기형발생율 (%) | 난산발생율 (%) | 위약송아지 (%) |
종래방법1 | 98/245 (40.0%) | 9/98 (9.2%) | 36/89 (40.4%) | 4/125 (3.2%) | 7/89 (7.9%) | 12/121 (9.9%) |
종래방법2 | 333/792 (42.0%) | 57/333 (17.0%) | 109/276 (39.1%) | 20/385 (5.2%) | 54/276 (19.6%) | 54/365 (14.8%) |
본 발명방법1 | 392/712 (55.1%) | 40/392두 (10.2%) | 193/372 (51.9%) | 11/565두 (1.9%) | 32/372두 (8.6%) | 30/554 (5.4%) |
본 발명방법2 | 119/216두 (55.1%) | 10/119두 (8.4%) | 67/109 A (61.5%) | 시험중 | 시험중 | 시험중 |
Claims (13)
- 이식용 스트로(straw) 내에 인간을 제외한 동물의 수정란을 봉입하는 방법에 있어서,공기 흡입단계, 수정란 흡입단계 및 공기 흡입단계를 포함하는 수정란 봉입 구획 설정 과정을 2회 이상 수행하고, 각 수정란 봉입 구획 설정 과정 사이에 착상증진물질을 흡인하는 단계를 포함하는 수정란 비봉입 구획 설정 과정을 수행함으로써 복수의 수정란 봉입 구획을 설정하는 것을 특징으로 하는 이식용 스트로 내에 수정란을 독립적으로 봉입하는 방법.
- 삭제
- 삭제
- 제1항에 있어서, 상기 각각의 수정란 봉입 구획 내에 1개 이상의 수정란을 봉입함을 특징으로 하는 방법.
- 제1항에 있어서, 상기 착상증진물질은 인터페론, 성호르몬, 비스테로이드 물질 또는 단백분해효소 중 선택된 어느 하나 이상인 것을 특징으로 하는 방법.
- 제1항, 제4항 및 제5항 중 어느 한 항에 따른 방법으로 이식용 스트로 내 봉입된 수정란을 피스톤이 부착된 이식기를 사용하여, 봉입 구획 단위별로 이식하는 것을 특징으로 하는 인간을 제외한 동물의 수정란 이식방법.
- 제6항에 있어서, 상기 이식기를 사용한 수정란 이식 전에, 상기 수정란이 봉입된 스트로우를 외피관 내에 포함시키고, 상기 외피관의 양 말단을 밀봉하여 이식용 스트로우를 보관 및 이동하는 과정을 더 포함하는 방법.
- 제7항에 있어서, 상기 외피관은 두께 0.01-0.2mm, 직경 0.5cm-1cm, 길이 10cm -20cm인 것을 특징으로 하는 폴리비닐관인 것을 특징으로 하고, 상기 이식용 스트로우가 포함된 외피관은 36.5-40.0℃로 보관하며, 상기 외피관은 수평을 유지하며 이동하는 것을 특징으로 하는 방법.
- 제6항에 있어서, 상기 수정란 이식은 수정란 봉입 구획 전.후방의 수정란 비봉입구획 내 물질을 함께 주입 또는 이식하는 것을 특징으로 하는 방법.
- 제6항에 있어서, 상기 수정란 봉입 구획 단위별 이식 또는 주입은 상기 피스톤 후반부에 수정란 봉입 구획 및 비봉입 구획을 단위별로 표시하고, 1 단위의 수 정란 봉입 구획을 포함하여 비봉입 구획 내의 일부 지점까지 피스톤을 진입시킴으로써 수행됨을 특징으로 하는 수정란 이식방법.
- 제6항에 있어서, 상기 동물은 포유동물인 것을 특징으로 하는 방법.
- 제11항에 있어서, 상기 포유동물은 소인 것을 특징으로 하는 방법.
- 제12항에 있어서, 상기 독립된 수정란 봉입 구획 내 수정란을 소의 양측 자궁강 내에 각각 독립된 구획 단위별로 이식하는 방법.
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KR1020080046663A KR100874875B1 (ko) | 2008-05-20 | 2008-05-20 | 이식용 스트로 내 단위 구획별 수정란 봉입방법과 이식방법 |
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Cited By (1)
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KR101289383B1 (ko) | 2010-02-08 | 2013-07-29 | 광개토한우농업법인 주식회사 | 소 생식세포와 임신증진물질을 동시 장착 후 1회로 자궁내에 주입이 가능한 2단 인공수정기 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
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KR100523222B1 (ko) * | 2003-09-04 | 2005-10-24 | 주식회사 에이비에스인코리아 | 수정란 이식용 스트로우 |
WO2007036627A1 (fr) * | 2005-09-28 | 2007-04-05 | Cryo Bio System | Ensemble de conditionnement d'un volume predetermine de substance a conserver par vitrification cryogenique |
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KR100523222B1 (ko) * | 2003-09-04 | 2005-10-24 | 주식회사 에이비에스인코리아 | 수정란 이식용 스트로우 |
WO2007036627A1 (fr) * | 2005-09-28 | 2007-04-05 | Cryo Bio System | Ensemble de conditionnement d'un volume predetermine de substance a conserver par vitrification cryogenique |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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KR101289383B1 (ko) | 2010-02-08 | 2013-07-29 | 광개토한우농업법인 주식회사 | 소 생식세포와 임신증진물질을 동시 장착 후 1회로 자궁내에 주입이 가능한 2단 인공수정기 |
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