KR100772054B1 - Composition for sterilization of pathogenic avian influenza - Google Patents

Composition for sterilization of pathogenic avian influenza Download PDF

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KR100772054B1
KR100772054B1 KR1020060084202A KR20060084202A KR100772054B1 KR 100772054 B1 KR100772054 B1 KR 100772054B1 KR 1020060084202 A KR1020060084202 A KR 1020060084202A KR 20060084202 A KR20060084202 A KR 20060084202A KR 100772054 B1 KR100772054 B1 KR 100772054B1
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weight
parts
composition
avian influenza
dissolve
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KR1020060084202A
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Korean (ko)
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김고성
이기용
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김고성
이기용
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Priority to PCT/KR2007/001090 priority patent/WO2007102688A1/en
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/14—Boron; Compounds thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/16—Heavy metals; Compounds thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
    • A61L2/16—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using chemical substances
    • A61L2/18—Liquid substances or solutions comprising solids or dissolved gases
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L9/00—Disinfection, sterilisation or deodorisation of air
    • A61L9/01—Deodorant compositions

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  • Life Sciences & Earth Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Inorganic Chemistry (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

An avian influenza sterilizing composition is provided to be freely sprayed to a place with foul odor in a gel, spray, dispenser or aerosol form, thereby removing foul odor and disinfecting foul odor generating places such as a stable. A composition for sterilizing pathogenic avian influenza comprises 40-60 parts by weight of sodium metasilicate, 45-70 parts by weight of potassium carbonate, 1-4 parts by weight of potassium citrate, 1-3 parts by weight of borax, and 10-60 parts by weight of white sugar regarding 100 parts by weight of water. A method for preparing the composition comprises the steps of: (a) putting 40-60 parts by weight of sodium metasilicate in 100 parts by weight of water at a temperature of 80-100 deg.C with heating and uniformly agitating to dissolve it; (b) putting 45-70 parts by weight of potassium carbonate in the solution obtained from the step(a) with uniformly agitating to dissolve it; (c) putting 1-4 parts by weight of potassium citrate in the solution obtained from the step(b) with uniformly agitating to dissolve it; (d) putting 1-3 parts by weight of borax in the solution obtained from the step(c) with uniformly agitating to dissolve it; and (e) putting 10-60 parts by weight of white sugar in the solution obtained from the step(d) with uniformly agitating to dissolve it.

Description

조류 인플루엔자 소독용 조성물{Composition for sterilization of pathogenic avian influenza}Composition for sterilization of avian influenza {Composition for sterilization of pathogenic avian influenza}

본 발명은 조류 축사 주변과 각종 축산기구 및 시설물을 소독하기 위한 조류 인플루엔자 소독용 조성물에 관한 것이다.The present invention relates to a composition for disinfecting avian influenza for disinfecting the surrounding birds and various livestock equipment and facilities.

종래 가축용 축사 주변과 축산기구 및 시설물을 살균, 소독하기위하여 가성소다, 소석회를 혼합유화한 농알카리성 수용액에 알카리에 안전한 항료를 혼화시킨 복합살균소독제 및 나푸톨색소를 첨가한 축사소독제가 개발되었으나, 이는 살균소독력이 약할 뿐만 아니라 소석회에 의해 축사 등의 시설물에 퇴적물이 쌓이게 되는 폐단이 있어 거의 실용화되지 못한 채 사장된 상태에 있다.In order to sterilize and disinfect livestock barns and livestock equipment and facilities, a complex disinfectant and a naphthol pigment were added to an alkaline alkaline solution mixed with caustic soda and hydrated lime and a naphthol pigment. In addition, the sterilization power is weak, and the deposits accumulate in facilities such as barns by slaked lime.

이는 가축용(축사 및 기타 시설물 포함) 살균, 소독제의 수요가 극히 미미하여 체산성이 없고, 대중적이지 못하기 때문에 개발이 지연된 것으로 추정된다.It is estimated that the development is delayed because the demand for sterilization and disinfectant for livestock (including livestock and other facilities) is very small and it is not acidified and not popular.

한편, 조류 인플루엔자는 닭, 오리, 칠면조 및 야생조류 등에 감염되는 급성 바이러스성 질병으로 병원성이 없는 것에서부터 치사율이 100%인 고병원성까지 다 양하게 있으며, 고병원성 조류인플루엔자는 국내에서 제1종 가축전염병, OIE(국제수역사무국)에서 리스트 A 등급으로 분류하고 있다. 원인체인 조류 인플루엔자 바이러스는 인수 공통바이러스로, 병원성에 따라 고병원성, 약병원성, 비병원성으로 구분하며, 혈철형(HA 단백질 15종류, NA 단백질 9종류)에 따라 135종류로 분류할 수 있다. 최근 사람에게 전파된 조류인플루엔자 바이러스로는 H5N1, H9N2 등이 있으며, 조류인플루엔자 바이러스는 비말(飛沫), 공기, 물 등에 의하여 전파되나, 주요 전파요인은 오염된 분변인데, 즉, 사람의 손, 발, 사료차, 기구, 장비 등에 분변이 묻어 간접적으로 전파된다.On the other hand, avian influenza is an acute viral disease that infects chickens, ducks, turkeys and wild birds, ranging from non-pathogenic to highly pathogenic with a 100% mortality rate. Classified as List A by the OIE. The causative agent of avian influenza virus is a common acquired virus, which is classified as highly pathogenic, weakly pathogenic, and non-pathogenic depending on pathogenicity, and can be classified into 135 types according to hemotype (15 types of HA protein and 9 types of NA protein). Avian influenza viruses recently transmitted to humans include H5N1 and H9N2. Avian influenza viruses are transmitted by droplets, air, and water, but the main propagation factor is contaminated feces, ie, human hands and feet. Feces can be found on food, tea, appliances, and equipment and spread indirectly.

또한 조류인플루엔자 바이러스가 혈청형이 다양하고 변이가 심하여 현재까지 효과적인 예방약이 개발되어 있지 않다. 따라서 대부분의 국가에서는 고병원성 조류인플루엔자 바이러스에 대하여 전량 도살 처분 정책을 실시하고 있으며, 발생국가에는 양계산물을 수출할 수 없게 된다. 농가에서는 철저한 출입통제 및 소독 등 차단방역만이 최선의 예방대책이나, 축사나 시설물, 기구 또는 주변환경을 소독하기 위한 이렇다할 소독제가 없는 실정이다.In addition, avian influenza virus has a wide range of serotypes and variations, so no effective preventive drugs have been developed. Therefore, most countries have a total slaughter policy against highly pathogenic avian influenza virus, and they cannot export poultry products to the developing country. In farmhouses, only preventive measures such as thorough access control and disinfection do not have the best preventive measures or such disinfectants to disinfect barns, facilities, equipment or the surrounding environment.

이에, 본 발명자는 상기와 같은 문제점을 해결하기 위해, 비용은 저렴하고 인체나 환경에는 전혀 무해하면서, 축사 및 기타 시설물의 악취 제거와 소독을 동시에 수행할 수 있는 조류 인플루엔자 소독용 조성물을 개발하고자 하였으며, 메타규산소다, 탄산칼슘, 구연산칼륨 및 붕사를 특정 배합 배율로 이루어지는 조성물 이, 조류 인플루엔자 바이러스인 H5A1 및 H5N1의 예방 효과와 악취 제거효과가 있음을 입증하여, 본 발명을 완성하였다.Accordingly, the present inventors have attempted to develop a composition for disinfecting avian influenza, which is inexpensive and harmless to the human body or the environment, and can simultaneously perform odor removal and disinfection of barns and other facilities. The composition consisting of sodium metasilicate, calcium carbonate, potassium citrate, and borax in a specific compounding ratio proved that the avian influenza viruses H5A1 and H5N1 had a prophylactic effect and an odor removing effect, thereby completing the present invention.

따라서, 본 발명의 목적은 메타규산소다, 탄산칼륨, 구연산칼륨 및 붕사로 이루어지는 조류 인플루엔자 소독용 조성물을 제공하는 것이다.Accordingly, it is an object of the present invention to provide a composition for disinfecting avian influenza comprising sodium metasilicate, potassium carbonate, potassium citrate and borax.

또한, 상기 조성물의 효과를 배가하기 위하여, 질산은, 염화금 또는 정백당을 더 첨가한 조류 인플루엔자 소독용 조성물을 제공하는 것이다.In addition, in order to double the effect of the composition, nitric acid is to provide a composition for disinfecting avian influenza further added gold chloride or white sugar.

상기와 같은 목적을 달성하기 위하여, 본 발명은 물 100 중량부에, 메타규산소다 40~60 중량부, 탄산칼륨 45~70 중량부, 구연산칼륨 1~4 중량부, 붕사 1~3 중량부를 포함하여 이루어지는 조류 인플루엔자 소독용 조성물을 제공한다.In order to achieve the above object, the present invention includes 100 parts by weight of water, 40 to 60 parts by weight of sodium metasilicate, 45 to 70 parts by weight of potassium carbonate, 1 to 4 parts by weight of potassium citrate, 1 to 3 parts by weight of borax. It provides a composition for disinfecting avian influenza.

이때, 본 발명에서 물의 중량부 측정은 단위의 편리성을 위하여, 밀도가 1인 순수한 물임을 가정하여 산출한 양을 사용한다.In this case, in the present invention, the weight part of water is measured for the convenience of the unit, using the amount calculated on the assumption that the density is 1 pure water.

또한, 또 다른 목적을 달성하기 위하여, 본 발명은 (a) 물 100 중량부를 80~100℃로 가열하여 유지하면서, 메타규산소다 40~60 중량부를 투입하고 균일하게 교반하여 용해시키는 단계;In addition, in order to achieve another object, the present invention (a) 40 to 60 parts by weight of sodium metasilicate while maintaining 100 parts by weight of water heated to 80 ~ 100 ℃ and uniformly stirred to dissolve;

(b) 상기 (a)의 용액에 탄산칼륨 45~70 중량부를 투입하고 균일하게 교반하여 용해시키는 단계;(b) adding 45 to 70 parts by weight of potassium carbonate to the solution of (a) and stirring to dissolve uniformly;

(c) 상기 (b)의 용액에 구연산칼륨 1~4 중량부를 투입하고 균일하게 교반하여 용해시키는 단계; 및(c) adding 1 to 4 parts by weight of potassium citrate to the solution of (b) and stirring to dissolve uniformly; And

(d) 상기 (c) 용액에 붕사 1~3 중량부를 투입하고 균일하게 교반하여 용해시키는 단계;(d) adding 1 to 3 parts by weight of borax to the solution (c) and stirring to dissolve uniformly;

로 제조되는 조류 인플루엔자 소독용 조성물을 제공한다.It provides a composition for disinfecting avian influenza prepared by.

이하, 본 발명을 상세히 설명하기로 한다.Hereinafter, the present invention will be described in detail.

이때, 사용되는 기술 용어 및 과학 용어에 있어서 다른 정의가 없다면, 이 발명이 속하는 기술 분야에서 통상의 지식을 가진 자가 통상적으로 이해하고 있는 의미를 가진다.At this time, if there is no other definition in the technical terms and scientific terms used, it has a meaning commonly understood by those of ordinary skill in the art.

또한, 종래와 동일한 기술적 구성 및 작용에 대한 반복되는 설명은 생략하기로 한다.In addition, repeated description of the same technical configuration and operation as in the prior art will be omitted.

본 발명에 따른 조류 인플루엔자 소독용 조성물은 사용 후 인체 및 동물에 무해한 것으로, 그 자체 또는 도료, 콘크리트, 합성수지 및 종이 등의 각종 기재에 사용하여, 축사 등의 악취 유발 장소에 악취를 제거하고 세균 및 곰팡이 등이 발생되지 않게 한 것이다.The composition for disinfecting avian influenza according to the present invention is harmless to humans and animals after use, and is used on its own or on various substrates such as paint, concrete, synthetic resin, and paper, to remove odors at odor causing places such as barns, and Mold is not generated.

이때, 본 발명은 상기 조성물을 유효성분으로 하고, 여기에 물 등의 희석제를 사용하여 원하는 농도로 조절한 다음, 겔, 분무, 디스펜서, 에어로졸 형태 등의 살포 방법으로 소독의 부위에 자유롭게 사용할 수 있으며, 축사 소독제로 사용 시에는 1000: 1 로 희석하여 사용하되, 분뇨 소독 시에는 500:1로 희석하여 사용하는 것이 효과적으로 바람직하다.At this time, the present invention uses the composition as an active ingredient, it is adjusted to a desired concentration using a diluent such as water, and then can be freely used at the site of disinfection by spraying method, such as gel, spray, dispenser, aerosol form, When used as a livestock disinfectant, dilute to 1000: 1, but dilute to 500: 1 when manure is disinfected.

또한, 본 발명에 따른 조성물을 축사에 사용하면, 악취는 제거하면서 분뇨 등을 분해하는 유익 미생물의 활성을 촉진하여, 분뇨 등의 축산 폐수를 처리하는 정화조로 유입되기 전에 일차 분해를 유도하여 폐수의 처리 효율을 향상시킬 수 있다.In addition, when the composition according to the present invention is used in a livestock house, it promotes the activity of beneficial microorganisms that decompose manure while removing odors, thereby inducing primary decomposition before entering the septic tank for treating livestock waste water such as manure. Processing efficiency can be improved.

본 발명에서 사용하는 물은 가급적 불순물을 정제한 정제수를 사용하는 것이 바람직하다.As the water used in the present invention, it is preferable to use purified water purified from impurities.

본 발명에서 사용하는 메타규산소다(Sodium meta silicate;Na2SiO3)는 알칼리 빌더로서 경도(硬度, 알카리성)과 pH를 높여서 요구되는 탄산칼슘의 사용량을 충분히 용해될 수 있도록 하고, 알칼리 조건을 유지하여 세정력을 강화하고 지방을 중화시켜 무취 상태로 유지하며, 계면활성력을 촉진하여 미생물의 침투력과 살균 효과를 상승시켜 주는 역할을 한다. 이때, 본 발명의 조성물에서 물 100 중량부 기준으로 메타규산소다를 40 중량부 미만을 사용하면 상기 역할의 효과가 저감되고, 60 중량부를 초과하면 인체나 동물의 피부나 점막 또는 눈 등에 자극을 줄 수 있기 때문에, 상술한 범위 내에서 사용하는 것이 바람직하다.Sodium meta silicate (Na 2 SiO 3 ) used in the present invention is an alkali builder to increase the hardness (alkalineity) and pH, so that the required amount of calcium carbonate can be sufficiently dissolved, maintaining the alkaline conditions It enhances cleansing power, neutralizes fat, keeps it odorless, and promotes surface active power to increase microbial penetration and sterilization effect. At this time, using less than 40 parts by weight of sodium metasilicate on the basis of 100 parts by weight of water in the composition of the present invention, the effect of the role is reduced, if more than 60 parts by weight give irritation to the skin or mucous membranes or eyes of the human body or animal Since it can be used, it is preferable to use within the range mentioned above.

그리고, 본 발명에서 탄산칼륨(Potassium carbonate;K2CO3)는 백색분말 형태의 저렴한 알칼리 소독제로서, 악취를 발생하거나 질병을 전염하는 바이러스나 세균 등을 소독하여 제거하는 역할을 하는데, 본 발명의 조성물에서 물 100 중량부 기준으로 45 중량부 미만을 사용하면 상술한 소독 효과가 저감되고 70 중량부를 초과하면 인체나 동물의 피부나 점막 또는 눈 등에 자극을 줄 수 있기 때문에, 상술한 범위 내에서 사용하는 것이 바람직하다.In the present invention, potassium carbonate (K2CO3) is an inexpensive alkali disinfectant in the form of white powder, and serves to disinfect and remove viruses or bacteria that cause odors or transmit diseases, and in the composition of the present invention, water The use of less than 45 parts by weight based on 100 parts by weight reduces the above-mentioned disinfection effect, and if it exceeds 70 parts by weight, it may cause irritation to the skin, mucous membranes or eyes of a human body or an animal. Do.

구연산칼륨(Potassium citrate; C6H5K3O7)은 탄산칼륨과 함께 칼륨이 이온화되면서 경도를 높이고, 자체의 살균 작용으로 소독 효과를 보다 상승시키며, 건축 기재가 부식되는 것을 방지하고, 탄산칼륨과 메타규산소다의 사용으로 인하여 지나치게 조성물이 알카리화되는 것을 억제해 주는 pH 조절제의 역할을 한다. 이때, 본 발명의 조성물에서 물 100 중량부 기준으로 구연산칼륨을 1 중량부 미만으로 사용하면 상술한 효과가 미미하고, 4 중량부를 초과하여 사용하면 초과 사용량에 비하여 효과가 미미하고 오히려 충분히 용해되지 않아 침전되어 조성물이 탁하게 되는 문제점이 발생할 수 있기 때문에, 상술한 범위 내에서 사용하는 것이 바람직하다.Potassium citrate (C 6 H 5 K 3 O 7 ), together with potassium carbonate, increases the hardness as potassium is ionized, increases its disinfection effect by its sterilization action, prevents corrosion of building substrates, The use of potassium and sodium metasilicate acts as a pH adjuster that inhibits excessive alkalinization of the composition. At this time, when the potassium citrate is used in an amount of less than 1 part by weight based on 100 parts by weight of water in the composition of the present invention, the above-described effects are insignificant. The problem of precipitation and turbidity of the composition may occur, so it is preferable to use it within the above-mentioned range.

붕사(Borex;Na2B4O7)는 살균 및 방부의 역할을 하여, 유기성 폐기물을 분해하는 미생물을 소독하거나 유기성 폐기물를 방부하여 부패되지 않게 하여 악취를 억제하고 지방과 유분을 유화하여 지방의 산화로 인한 악취를 방지하는 역할을 하는데, 본 발명의 조성물에서는 물 100 중량부 기준으로 1 중량부 미만을 사용하면 상술한 효과를 달성할 수 없고, 3 중량부를 초과하여 사용하면 충분히 용해되지 않고 침전되어 조성물이 탁해질 수 있기 때문에, 상술한 범위 내에서 사용하는 것이 바람직하다.Borax (Borex; Na 2 B 4 O 7 ) plays a role in sterilization and antiseptic, disinfecting microorganisms that decompose organic wastes or preserving organic wastes to prevent decay. It serves to prevent the odor due to, in the composition of the present invention using less than 1 part by weight based on 100 parts by weight of water can not achieve the above-described effect, when used in excess of 3 parts by weight is precipitated without being sufficiently dissolved Since the composition may become cloudy, it is preferable to use it within the above-mentioned range.

이와 같이, 본 발명의 조성물은 칼륨염을 위주로 사용함으로써, 구성 성분간에 서로 반응이 잘 되도록 유도한 것이다. As described above, the composition of the present invention is derived by using potassium salt mainly, so that the components react well with each other.

또한, 본 발명에서 사용하는 질산은(Silver nitrate;AgNO3)과 염화금(Gold chloride;AuCl)은 성분 자체가 물에서 이온화되어 은과 금을 석출하여 살균과 악취 를 분해하여 탈취하는 효과를 나타내어, 본 발명의 조성물에 소독 및 탈취의 효과를 보조하는 역할을 하는데, 특히, 두 성분을 병용하여 소독 및 탈취의 시너지 효과를 얻는 것이 바람직하다. 이때, 본 발명에서는 물 100중량부에 대하여 각각 0.03~0.04 중량부를 사용하는 것이 바람직한데, 이는 0.03 중량부 미만을 사용하면 상술한 효과가 미미하고, 0.04 중량부 초과하여 사용하면 첨가 사용량에 비하여 효과가 미미하고, 고가의 물질을 다량사용하여 제조 단가가 높일 필요가 없기 때문이다.In addition, the silver nitrate (AgNO 3 ) and gold chloride (AuCl) used in the present invention, the components themselves are ionized in water to precipitate silver and gold to decompose sterilization and odor to deodorize, The composition of the invention serves to assist the effect of disinfection and deodorization, in particular, it is preferable to use the two components in combination to obtain the synergistic effect of disinfection and deodorization. At this time, in the present invention, it is preferable to use 0.03 to 0.04 parts by weight with respect to 100 parts by weight of water, respectively, which is less than the above-described effects when using less than 0.03 parts by weight, and when used in excess of 0.04 parts by weight compared to the amount of addition This is because it is insignificant and the manufacturing cost does not need to be increased by using a large amount of expensive materials.

그리고, 본 발명에서 정백당(sugar)는 축사 등의 악취 유발 장소에서 유기물을 발효하는 미생물의 활성을 촉진하여 악취의 원인인 질소화합물이나 황 함유 화합물의 생성을 억제하고, 이의 악취를 제거하는 역할을 한다. 이때, 본 발명에서는 정백당을 10~60 중량부를 사용하는 것이 바람직한데, 이는 본 발명의 조성물에서 물 100 중량부 기준으로 10 중량부 미만을 사용하면 상기 효과가 저감되고, 60 중량부를 초과하여 사용하면 오히려 부패균을 촉진하여 악취를 더욱 유발하는 문제점이 발생할 수 있기 때문이다.In the present invention, the white sugar (sugar) serves to suppress the production of nitrogen compounds or sulfur-containing compounds that cause odors by promoting the activity of microorganisms that ferment organic matters in odor-inducing places such as barns, and removes odors thereof. do. At this time, in the present invention, it is preferable to use 10 to 60 parts by weight of white sugar, which is less than 10 parts by weight based on 100 parts by weight of water in the composition of the present invention, the effect is reduced, if used in excess of 60 parts by weight Rather, because it promotes the decay bacteria may cause a problem that causes more odor.

한편, 본 발명에 의한 조류 인플루엔자 소독용 조성물의 제조방법은 본 발명의 구성성분이 균일하게 용해될 수 있도록, 물을 80~100℃로 가열한 상태에서 메타규산소다, 탄산칼륨, 구연산 칼륨 및 붕사의 순으로 투입하고 완전히 용해될 때까지 다음 성분을 투입하지 않는 것이 바람직하다. 이는 각각 투입하여 용해시킬 경우, 모든 구성성분이 균일하게 용해되면서, 보관 시 미 반응된 성분 간의 반응으로 침전물이 발생하지 않기 때문이다.On the other hand, the method for producing a composition for disinfecting algae influenza according to the present invention, sodium metasilicate, potassium carbonate, potassium citrate and borax in a state in which water is heated to 80 to 100 ℃ so that the components of the present invention can be uniformly dissolved. It is preferable to add in the following order and not add the next ingredient until it is completely dissolved. This is because, when each is added and dissolved, all of the components are uniformly dissolved, and no precipitate is generated by the reaction between the unreacted components during storage.

또한, 상기의 용액에 질산은, 염화금 또는 정백당을 순서대로 더 투여하고 완전히 용해시켜 사용하는 것이 바람직하다.In addition, it is preferable to use silver nitrate in order to further administer gold chloride or white sugar, and to dissolve completely.

그러나, 본 발명의 제조방법은 바람직한 일 실시예의 방법일 뿐, 본 발명이 상술한 제조방법으로 국한되는 것은 아니며, 이는 본 발명의 분야에서 통상의 지식을 가진 자라면 용이하게 알 수 있는 자명한 것이다.However, the manufacturing method of the present invention is not only the method of the preferred embodiment, but the present invention is not limited to the above-described manufacturing method, which will be apparent to those skilled in the art. .

이하, 본 발명을 구체적인 실시예에 의해 보다 더 상세히 설명하고자 한다. 하지만, 본 발명은 하기 실시예에 의해 한정되는 것은 아니며, 본 발명의 사상과 범위 내에서 여러 가지 변형 또는 수정할 수 있음은 이 분야에서 당업자에게 명백한 것이다.Hereinafter, the present invention will be described in more detail with reference to specific examples. However, the present invention is not limited to the following examples, and it will be apparent to those skilled in the art that various changes or modifications can be made within the spirit and scope of the present invention.

[실시예 1] Example 1

90℃ 이상으로 가열한 물 100ℓ에 메타규산소다(Na2SiO3) 45 kg을 투입하여 균일하게 용해하고, 상기 용액에 탄산칼륨(K2CO3) 50 kg을 투입하고 용해한 다음, 구연산칼륨(C6H5K3O7) 2 kg을 투입하여 다시 균일하게 용해하고, 붕사(Na2B4O7) 1.33 kg를 투입하여 완전히 용해시켜, 본 발명의 조류 인플루엔자 소독용 조성물을 제조하였다.45 kg of sodium metasilicate (Na 2 SiO 3 ) was added to 100 l of water heated to 90 ° C. or higher to dissolve uniformly, and 50 kg of potassium carbonate (K 2 CO 3 ) was added to the solution and dissolved therein, followed by potassium citrate ( 2 kg of C 6 H 5 K 3 O 7 ) was added to dissolve uniformly, and 1.33 kg of borax (Na 2 B 4 O 7 ) was added to dissolve completely to prepare a composition for disinfecting avian influenza of the present invention.

[실시예 2]Example 2

상기 실시예 1과 동일한 방법으로 90℃ 이상으로 가열한 물 100ℓ에 메타규산소다(Na2SiO3) 45 kg, 탄산칼륨 50 kg, 구연산칼륨 2 kg, 붕사 1.33 kg 및 염화금(AuCl) 0.033g을 투입하여 용해하고, 마지막으로 정백당(C12H22O11) 46.7 kg을 투입하고 완전히 용해시켜, 본 발명의 조류 인플루엔자 소독용 조성물을 제조하였다. 45 kg of sodium metasilicate (Na 2 SiO 3 ), 50 kg of potassium carbonate, 2 kg of potassium citrate, 1.33 kg of borax and 0.033 g of gold chloride (AuCl) were added to 100 l of water heated to 90 ° C. or higher in the same manner as in Example 1. It was added and dissolved, and finally 46.7 kg of white sugar (C 12 H 22 O 11 ) was added and completely dissolved to prepare a composition for disinfecting avian influenza of the present invention.

이때, 본 발명의 조성물의 비중은 1.49, pH는 13.78이었다.At this time, the specific gravity of the composition of the present invention was 1.49, pH was 13.78.

[실시예 3]Example 3

상기 실시예 1과 동일한 방법으로, 90℃ 이상으로 가열한 물 100ℓ에 메타규산소다(Na2SiO3) 45 kg, 탄산칼륨 46.7 kg, 구연산칼륨 2 kg, 붕사 2 kg 및 정백당 30 kg을 차례대로 투입하고 용해시켜, 본 발명의 조류 인플루엔자 소독용 조성물을 제조하였다. In the same manner as in Example 1, 45 kg of sodium metasilicate (Na 2 SiO 3 ), 46.7 kg of potassium carbonate, 2 kg of potassium citrate, 2 kg of borax and 30 kg of white spirit were sequentially added to 100 l of water heated to 90 ° C. or higher. It was added and dissolved to prepare a composition for disinfecting avian influenza of the present invention.

이때, 본 발명의 조성물의 비중은 1.48, pH는 14.6이었다.At this time, the specific gravity of the composition of the present invention was 1.48, pH was 14.6.

[실시예 4]Example 4

상기 실시예 1과 동일한 방법으로, 90℃ 이상으로 가열한 물 100ℓ에 메타규산소다(Na2SiO3) 55 kg, 탄산칼륨 65 kg, 구연산칼륨 3 kg, 붕사 2.5 kg 및 질산은(AgNO3) 0.033g을 차례대로 투입하고 용해시켜, 본 발명의 조류 인플루엔자 소독용 조성물을 제조하였다.In the same manner as in Example 1, 55 kg of sodium metasilicate (Na 2 SiO 3 ), 65 kg of potassium carbonate, 3 kg of potassium citrate, 2.5 kg of borax and silver nitrate (AgNO 3 ) in 100 l of water heated to 90 ° C. or higher. g was added sequentially and dissolved to prepare a composition for disinfecting avian influenza of the present invention.

이때, 본 발명의 조성물의 비중은 1.46, pH는 14.8이었다.At this time, the specific gravity of the composition of the present invention was 1.46, pH was 14.8.

[실시예 5]Example 5

상기 실시예 1과 동일한 방법으로, 90℃ 이상으로 가열한 물 100ℓ에 메타규산소다(Na2SiO3) 43.3 kg, 탄산칼륨 46.7 kg, 구연산칼륨 1.67 kg, 붕사 2.67 kg 및 정백당 17 kg을 차례대로 투입하고 용해시켜, 본 발명의 조류 인플루엔자 소독용 조성물을 제조하였다.In the same manner as in Example 1, 43.3 kg of sodium metasilicate (Na 2 SiO 3 ), 46.7 kg of potassium carbonate, 1.67 kg of potassium citrate, 2.67 kg of borax and 17 kg of white spirit were sequentially added to 100 l of water heated to 90 ° C. or higher. It was added and dissolved to prepare a composition for disinfecting avian influenza of the present invention.

이때, 본 발명의 조성물의 비중은 1.41, pH는 13.85이었다.At this time, the specific gravity of the composition of the present invention was 1.41, pH was 13.85.

[실험예 1]Experimental Example 1

본 발명의 조성물(실시예 3)을 증류수 1ℓ에 염화칼슘(CaCl2) 0.305g 및 염화마그네슘6수화물(MgCl26H2O) 0.139g을 용해시킨 경수에 200, 250, 300, 400, 500배로 희석한 후 하기의 방법에 따라 조류 인플루엔자 바이러스에 대한 소독 효력 시험을 하였다. 상기 표에 나타난 바와 같이, 본 발명에 따른 소독제를 희석하여 사용함으로써 조류 인플루엔자 바이러스를 예방할 수 있음을 알 수 있었다.The composition (Example 3) of the present invention was diluted 200, 250, 300, 400, 500 times in hard water in which 0.305 g of calcium chloride (CaCl 2 ) and 0.139 g of magnesium chloride hexahydrate (MgCl 2 6H 2 O) were dissolved in 1 L of distilled water. After that, the disinfection effect test for avian influenza virus was performed according to the following method. As shown in the table, it was found that by using dilution of the disinfectant according to the present invention can prevent avian influenza virus.

1. 사용된 균주 : Avian influeneza virus(H9N2, MS96, A/Chicken/Korea/S4/2003, 2005년 Virogy, 332; 529-537 발표)Strains used: Avian influeneza virus (H9N2, MS96, A / Chicken / Korea / S4 / 2003, 2005 Virogy, 332; published on 529-537)

2. 사용된 배지 : 10일령 유정란2. Medium used: 10-day-old fertilized egg

3. 바이러스 배양 : 10일령 유정란에 균주를 접종한고 48시간동안 배양한 후 요막강액을 수득하여 egg infectious dose 50를 측정한 후 100EID50의 바이러스 양을 효능시험에 이용.3. Virus culture: After inoculating the 10-day-old fertilized egg strain and incubating for 48 hours, the urinary fluid was obtained, the egg infectious dose 50 was measured, and the virus amount of 100EID50 was used for the efficacy test.

4. 소독제 반응 : 121℃에서 15분간 고압멸균시킨 경수(증류수 1ℓ+ CaCl2 0.305g + MgCl2 6H20 0.139g)에 소태아혈청 1%(w/v)을 용해시킨 유기물희석액 24.0㎖와 요막강액에서 증식한 바이러스 100EID50 1.0㎖를 혼합시켜 얻어진 혼합물 2.5㎖와 200, 250, 300, 400, 500배로 희석된 각각의 소독제 희석액 2.5㎖을 혼합한 후 4℃에서 30분간 반응시키고, 반응 도중에 10분마다 혼합하여 준다.4. Disinfectant reaction: 24.0 ml of organic diluent with 1% (w / v) of fetal bovine serum dissolved in hard water (distilled water 1ℓ + CaCl 2 0.305g + MgCl 2 6H 2 0 0.139g) autoclaved at 121 ° C for 15 minutes and 2.5 ml of the mixture obtained by mixing 1.0 ml of virus 100EID50 propagated in the urinary cavity fluid and 2.5 ml of each diluent diluted 200, 250, 300, 400, and 500 times were mixed and reacted at 4 ° C. for 30 minutes. Mix every 10 minutes.

5. 중화반응 : 소독제의 효능을 중화시키기 위하여 소독제 희석액과의 반응이 완료된 후 즉시 1.0㎖를 취하여 37℃의 중화배지에 첨가하여 혼합한 후 각 소독제 희석액 별로 0.2㎖씩 취하여 5개의 유정란에 접종하고, 37℃ 항온실에서 48시간동안 배양하고 요막강액을 수득하기 전에 12시간동안 4℃에서 냉장보관한다. 상기 중화배지는 MEM 영양배지에 불활화(56℃에서 30분 처리)한 말혈청 5%를 첨가한 용액 1 분량에 5% 비동화 말혈청 199 분량과 혼합하여 제조된다. 5. Neutralization: To neutralize the efficacy of the disinfectant, take 1.0 ml immediately after completion of the reaction with the disinfectant diluent, add it to a neutralizing medium at 37 ° C, mix it, take 0.2 ml of each disinfectant diluent, and inoculate it into five eggs. Incubate for 48 hours in a 37 ° C. constant temperature room and refrigerate at 4 ° C. for 12 hours before obtaining the lumen. The neutralizing medium is prepared by mixing 199 parts of 5% immobilized horse serum to 1 part of a solution to which 5% of horse serum inactivated (treated at 56 ° C. for 30 minutes) is added to MEM nutrient medium.

6. 증식여부 판정 : 바이러스의 증식은 혈구응집반응에 의해 결정된다.6. Judgment of the proliferation: The proliferation of the virus is determined by the hemagglutination reaction.

[표 1]TABLE 1

Figure 112006063474543-pat00001
Figure 112006063474543-pat00001

따라서, 본 발명에 따른 조류 인플루엔자 소독용 조성물은 조류 인플루엔자 바이러스를 예방하는데 효과가 있음을 알 수 있었다.Therefore, the composition for disinfecting avian influenza according to the present invention was found to be effective in preventing avian influenza virus.

이상과 같이, 본 발명은 인체, 동물에 대하여 안정하고 무해하면서, 겔, 분무, 디스펜서, 에어로졸 형태 등의 살포 방법으로 악취나 소독이 필요한 장소에 자유롭게 사용하거나, 도료, 콘크리트, 합성수지 및 종이 등의 각종 기재에 사용하여, 축사 등의 악취 유발 장소에 사용하여 악취 제거 및 소독효과를 제공하게 되었다.As described above, the present invention is stable and harmless to humans and animals, and can be freely used in places where odor or disinfection is necessary by spraying methods such as gel, spray, dispenser, aerosol, or the like, such as paint, concrete, synthetic resin, paper, etc. It is used in various substrates, and used in places of causing odors, such as barns, to provide odor removal and disinfection effects.

또한, 본 발명에 따른 조성물을 축사에 사용하면, 악취는 제거하면서 분뇨 등을 분해하는 유익 미생물의 활성을 촉진하여, 분뇨 등의 축산 폐수를 처리하는 정화조로 유입되기 전에 일차 분해를 유도하여 폐수의 처리 효율을 향상시킬 수 있을 뿐만 아니라 본 발명에 따른 조성물을 사용하여 조류 인플루엔자 바이러스를 예방할 수 있는 효과가 있다.In addition, when the composition according to the present invention is used in a livestock house, it promotes the activity of beneficial microorganisms that decompose manure while removing odors, thereby inducing primary decomposition before entering the septic tank for treating livestock waste water such as manure. Not only can the treatment efficiency be improved, but the composition according to the present invention has the effect of preventing avian influenza virus.

Claims (6)

물 100 중량부에,To 100 parts by weight of water, 메타규산소다 40~60 중량부, 탄산칼륨 45~70 중량부, 구연산칼륨 1~4 중량부, 붕사 1~3 중량부 및 정백당 10~60 중량부를 포함하여 이루어지는 조류 인플루엔자 소독용 조성물.A composition for disinfecting avian influenza comprising 40 to 60 parts by weight of sodium metasilicate, 45 to 70 parts by weight of potassium carbonate, 1 to 4 parts by weight of potassium citrate, 1 to 3 parts by weight of borax and 10 to 60 parts by weight per white spirit. 제 1 항에 있어서, The method of claim 1, 물 100 중량부에 대하여 질산은(Siver nitrate) 0.03~0.04 중량부를 더 포함하는 것을 특징으로 하는 조류 인플루엔자 소독용 조성물.A composition for disinfecting avian influenza, further comprising 0.03 to 0.04 parts by weight of silver nitrate based on 100 parts by weight of water. 제 1 항 또는 제 2 항에 있어서, The method according to claim 1 or 2, 물 100 중량부에 대하여 염화금(Gold chloride) 0.03~0.04 중량부를 더 포함하는 것을 특징으로 하는 조류 인플루엔자 소독용 조성물.Algae influenza disinfecting composition, characterized in that it further comprises 0.03 to 0.04 parts by weight of gold chloride with respect to 100 parts by weight of water. 삭제delete (a) 물 100 중량부를 80~100℃로 가열하여 유지하면서, 메타규산소다 40~60 중량부를 투입하고 균일하게 교반하여 용해시키는 단계; (A) 40 to 60 parts by weight of sodium metasilicate while maintaining 100 parts by weight of water heated to 80 ~ 100 ℃ and uniformly stirred to dissolve; (b) 상기 (a)의 용액에 탄산칼륨 45~70 중량부를 투입하고 균일하게 교반하여 용해시키는 단계;(b) adding 45 to 70 parts by weight of potassium carbonate to the solution of (a) and stirring to dissolve uniformly; (c) 상기 (b)의 용액에 구연산칼륨 1~4 중량부를 투입하고 균일하게 교반하여 용해시키는 단계;(c) adding 1 to 4 parts by weight of potassium citrate to the solution of (b) and stirring to dissolve uniformly; (d) 상기 (c) 용액에 붕사 1~3 중량부를 투입하고 균일하게 교반하여 용해시키는 단계; 및(d) adding 1 to 3 parts by weight of borax to the solution (c) and stirring to dissolve uniformly; And (e) 상기 (d) 단계의 용액에 정백당 10~60 중량부를 투입하고 균일하게 교반하여 용해시키는 단계;(e) adding 10 to 60 parts by weight of the white spirit to the solution of step (d) and stirring to dissolve uniformly; 를 포함하여 이루어지는 조류 인플루엔자 소독용 조성물의 제조방법.Method for producing a composition for disinfecting avian influenza comprising a. 제 5 항에 있어서,The method of claim 5, 상기 (e) 단계의 용액에 물 100 중량부에 대하여 질산은 0.03~0.04 중량부 또는 염화금 0.03~0.04 중량부를 투입하여 균일하게 교반하여 용해시키는 단계가 더 포함하는 것을 특징으로 하는 조류 인플루엔자 소독용 조성물의 제조방법.To the solution of step (e) is added to 0.03 ~ 0.04 parts by weight of silver nitrate or 0.03 ~ 0.04 parts by weight of gold chloride with respect to 100 parts by weight of water, the step of uniformly stirring to dissolve the influenza avian influenza composition Manufacturing method.
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KR20160142114A (en) 2015-06-02 2016-12-12 (주)이엘티사이언스 The composition of foot dip disinfectant for livestock quarantine and method for manufacturing thereof
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