KR100483863B1 - 고체분말을 이용한 3차원 세포 배양방법 - Google Patents
고체분말을 이용한 3차원 세포 배양방법 Download PDFInfo
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- KR100483863B1 KR100483863B1 KR10-2002-0085331A KR20020085331A KR100483863B1 KR 100483863 B1 KR100483863 B1 KR 100483863B1 KR 20020085331 A KR20020085331 A KR 20020085331A KR 100483863 B1 KR100483863 B1 KR 100483863B1
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- solid powder
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0653—Adipocytes; Adipose tissue
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0068—General culture methods using substrates
- C12N5/0075—General culture methods using substrates using microcarriers
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0655—Chondrocytes; Cartilage
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2531/00—Microcarriers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue
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- Health & Medical Sciences (AREA)
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- Life Sciences & Earth Sciences (AREA)
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- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
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- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
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- Materials For Medical Uses (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
Claims (10)
- 지방전구세포(preadipocyte), 연골모세포(chondroblast), 골모세포(osteoblast)와 같은 계대배양된 배양체 세포들을 크기가 20-500 ㎛인 기포 없는 고체 분말로 이루어진 배양소재와 함께 배양액에 부유시키는 방법으로 고체분말과 배양소재를 혼합하고 이를 배양기에 넣고 배양하여 고체분말과 배양소재가 부착되도록 하는 것을 특징으로 하는 고체분말을 이용한 3차원 세포 배양방법.
- 제1항에 있어서, 고체 분말은 인체로부터 얻어진 동종진피가루인 것을 특징으로 고체분말을 이용한 3차원 세포 배양방법.
- 제1항에 있어서, 고체 분말은 미세기포를 갖지 않는 키토산인 것을 특징으로 하는 고체분말을 이용한 3차원 세포 배양방법.
- 삭제
- 제1항에 있어서, 고체 분말은 인체 근막조직 가루인 것을 특징으로 하는 고체분말을 이용한 3차원 세포 배양방법.
- 제1항에 있어서, 고체 분말은 미세기포를 갖지 않는 고어텍스(PTFE) 가루인 것을 특징으로 고체분말을 이용한 3차원 세포 배양방법.
- 삭제
- 삭제
- 삭제
- 삭제
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR10-2002-0085331A KR100483863B1 (ko) | 2002-12-27 | 2002-12-27 | 고체분말을 이용한 3차원 세포 배양방법 |
PCT/KR2003/002842 WO2004058952A1 (en) | 2002-12-27 | 2003-12-24 | Method of culturing autologous cell using injectable solid powder |
AU2003289530A AU2003289530A1 (en) | 2002-12-27 | 2003-12-24 | Method of culturing autologous cell using injectable solid powder |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR10-2002-0085331A KR100483863B1 (ko) | 2002-12-27 | 2002-12-27 | 고체분말을 이용한 3차원 세포 배양방법 |
Publications (2)
Publication Number | Publication Date |
---|---|
KR20040058818A KR20040058818A (ko) | 2004-07-05 |
KR100483863B1 true KR100483863B1 (ko) | 2005-04-15 |
Family
ID=32677799
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR10-2002-0085331A KR100483863B1 (ko) | 2002-12-27 | 2002-12-27 | 고체분말을 이용한 3차원 세포 배양방법 |
Country Status (3)
Country | Link |
---|---|
KR (1) | KR100483863B1 (ko) |
AU (1) | AU2003289530A1 (ko) |
WO (1) | WO2004058952A1 (ko) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20030007954A1 (en) | 1999-04-12 | 2003-01-09 | Gail K. Naughton | Methods for using a three-dimensional stromal tissue to promote angiogenesis |
WO2006026652A2 (en) * | 2004-08-30 | 2006-03-09 | Iken Tissue Therapeutics, Inc. | Conditioned medium comprising wnt proteins to promote repair of damaged tissue |
KR101713475B1 (ko) | 2016-01-29 | 2017-03-07 | 류승호 | 연골을 포함하는 주사 이식용 신체 부피 대체재 |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5266480A (en) * | 1986-04-18 | 1993-11-30 | Advanced Tissue Sciences, Inc. | Three-dimensional skin culture system |
AU1931495A (en) * | 1994-03-14 | 1995-10-03 | Cryolife, Inc. | Treated tissue for implantation and preparation methods |
JP2002233567A (ja) * | 2000-12-06 | 2002-08-20 | Mitsuo Ochi | 移植用組織等価物及びその製造方法 |
WO2003009818A1 (fr) * | 2001-07-25 | 2003-02-06 | Applied Cell Biotechnologies, Inc. | Cosmetiques autologues et materiaux medicaux autologues et procede permettant de les produire |
CN1178989C (zh) * | 2001-07-30 | 2004-12-08 | 兴技生物科技股份有限公司 | 制备胶原蛋白和生物陶瓷粉末复合材料微粒的方法 |
KR100532562B1 (ko) * | 2002-01-09 | 2005-12-02 | 메디칸(주) | 주사제로 인체주입이 가능한 스펀지구조를 갖는 고체키토산분말과 그 제조방법 및 주입장치 |
-
2002
- 2002-12-27 KR KR10-2002-0085331A patent/KR100483863B1/ko active IP Right Grant
-
2003
- 2003-12-24 WO PCT/KR2003/002842 patent/WO2004058952A1/en not_active Application Discontinuation
- 2003-12-24 AU AU2003289530A patent/AU2003289530A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
AU2003289530A1 (en) | 2004-07-22 |
KR20040058818A (ko) | 2004-07-05 |
WO2004058952A1 (en) | 2004-07-15 |
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