KR100458487B1 - Animals fodder for composition Bacillus polyfermenticus - Google Patents

Animals fodder for composition Bacillus polyfermenticus Download PDF

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KR100458487B1
KR100458487B1 KR10-2002-0024577A KR20020024577A KR100458487B1 KR 100458487 B1 KR100458487 B1 KR 100458487B1 KR 20020024577 A KR20020024577 A KR 20020024577A KR 100458487 B1 KR100458487 B1 KR 100458487B1
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강재선
김원석
박유수
김혜진
전경동
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주식회사 바이넥스
이백천
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus

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Abstract

본 발명은 알을 낳는 동물의 경우에는 산란 량 증가와 폐사율의 개선시키고, 새끼를 낳는 동물의 경우에는 출산 수의 증가 효과를 나타내는 바실러스 폴리퍼멘티쿠스를 함유한 동물용 사료에 관한 것으로, 이는 동물의 산란율 증가, 출산율 증가, 폐사율 감소 효과를 위하여 동물사료에 바실러스 폴리퍼멘티쿠스(Bacillus polyfermenticus) 성분을 함유시켜 된 것이고 상기 바실러스 폴리퍼멘티쿠스 성분의 함유량은 동물사료 1톤당 바실러스 폴리퍼멘티쿠스를 1 ×109개(1 ×109cfu)∼ 5 ×1010cfu 이다.The present invention relates to an animal feed containing Bacillus polypermanticus, which shows an effect of increasing egg production and mortality in an egg-laying animal and an increase in the number of births in an animal. Bacillus polyfermenticus component is contained in the animal feed to increase the spawning rate, fertility rate, and mortality, and the content of Bacillus polyfermentus is 1% per ton of animal feed. 1 × 10 9 (1 × 10 9 cfu) to 5 × 10 10 cfu.

Description

바실러스 폴리퍼멘티쿠스를 함유한 동물용 사료{Animals fodder for composition Bacillus polyfermenticus}Animal feed containing Bacillus polyfermentus {Animals fodder for composition Bacillus polyfermenticus}

본 발명은 알을 낳는 동물의 경우에는 산란 량 증가와 폐사율의 개선시키고, 새끼를 낳는 동물의 경우에는 출산 수의 증가 효과를 나타내는 바실러스 폴리퍼멘티쿠스를 함유한 동물용 사료에 관한 것이다.The present invention relates to an animal feed containing Bacillus polypermanticus, which shows an effect of increasing egg production and mortality in the case of laying eggs and increasing the number of births in the case of pups.

일반적인 동물용 사료 첨가제로는 영양제 및 항균제 그리고 유산균을 사용한 예는 있으나, 상기 영양제를 첨가할 때에는 가격상승으로 인한 측면에서 불리하고, 또 효과적인 측면에서의 통계 수치가 증명되기 어려우며, 상기 항균제의 사용에서는 내성 및 잔류성 등의 문제로 인하여 유해균의 증가와 항균제의 남용 잔류성으로 인한 인체에 유해를 일으키는 등 사용상 제약이 많았으며, 기타 생균제의 복용에서는 생균제제의 안정성 결핍 및 고가인 점을 고려 할 때 뚜렷한 효과를 나타내기가어려웠다.Examples of general animal feed additives include nutrients, antimicrobials, and lactic acid bacteria, but the addition of the nutrients is disadvantageous due to the increase in price, and it is difficult to prove statistical values in terms of effectiveness. Due to problems such as resistance and persistence, there are many restrictions on the use of harmful bacteria such as the increase in harmful bacteria and the abuse of antimicrobial agents, which are harmful to the human body. It was difficult to indicate.

Bacillus polyfermenticus는 1933년에 일본의 Terakato가 공기로부터 분리한 내생포자(endospore)성 간균으로서 20여종의 소화효소를 분비하며 비타민 B1, B2, K, C를 합성하여 영양을 보급시킨다. 또 티프스균, 파라티프스균, 적리균, 콜레라균, 이질균, 비브리오, 아스페르길러스(황국국, 흑국균), 황색포도상구균, 대장균 0 157, 파상풍균(Clostridium) 등을 용균 또는 정균하는 것으로 본 발명자 등이 밝힌 바 있다. Bacillus polyfermenticus is an endospore bacillus isolated from air by Terakato in Japan in 1933. It secretes 20 digestive enzymes and synthesizes vitamins B1, B2, K, and C. Also, it is considered to be lysing or bacteriostatic bacteria such as Tiffus bacillus, Paratyphoid bacillus, Cyclobacterium, Cholera, Heterogeneous bacteria, Vibrio, Aspergillus, Yellow Staphylococcus aureus, Escherichia coli 0 157, and Clostridium. The inventors have disclosed.

또한 어류의 한 종류인 나일틸라피아를 대상으로 한 실험에서는 인위적으로 병원성 세균인 에드와드시엘라 타르다를 복강 주사하여( 5.7 ×107cfu/ 0.1 ml) 관찰한 결과로서Bacillus polyfermenticus를 복용한 시험군에서 폐사율이 51%정도로 대조군 80%에 비하여 현격히 개선됨을 보였으며 혈액 채취결과 면역 증강 상태를 보여주고 있었으며 구체적으로는 두신내 식세포의 활성산소 방출량 증가, 혈액내 식세포능 증가, 보체용혈능력 향상, 혈청 내 리소좀의 활성증가 등을 보여주었다.In addition, experiments involving Nile tilapia, a type of fish, were observed by intraperitoneal injection of artificial pathogenic bacterium Eddsiella tarda (5.7 × 10 7 cfu / 0.1 ml) in the test group taking Bacillus polyfermenticus . The mortality rate was about 51%, which was markedly improved compared to the 80% control group, and blood collection showed immunity-enhancing status. Increased activity of lysosomes.

바실러스 폴리퍼멘티쿠스가 생성하는 항균성 물질인 박테리오신, 초산 및 젓산의 작용으로 인하여 유해균을 박멸하는 결과를 보였다.(도3 참조)The bacteriocin, acetic acid and lactic acid, which act as antibacterial substances produced by Bacillus polyfermenticus, resulted in the elimination of harmful bacteria (see Fig. 3).

또, 위액과 담즙산에 안정하며 경구투여 후 장 도달율이 90% 이상이며 열이나 화학 물질에도 안정성을 보이므로 인하여 유통중에 품질이 균일하게 유지되며 화학요법제등과의 병용투여에 의한 체내 균총 파괴를 막을 수 있다는 장점이 있다.In addition, it is stable to gastric juice and bile acid, and the intestinal reach rate is more than 90% after oral administration, and it shows stability to heat and chemicals so that the quality is maintained uniformly during distribution and prevents the destruction of the flora in the body by co-administration with chemotherapeutic agents. There is an advantage that it can.

또한 유해균이 장내에서 영양분을 파괴하고 비타민의 분해를 일으켜 영양공급의 불균형을 초래하는데 바실러스 폴리퍼멘티쿠스가 생성하는 항균성 물질로 인해 이런 유해균을 죽이므로 인하여 생체가 영양을 흡수토록 하여 주며, 바실러스 폴리퍼멘티쿠스가 비타민을 합성하여 주고 소화효소를 분비하여 소화작용을 보이므로 인하여 영양의 효율을 높여 결국에는 이런 결과로 산란율 증가 등을 일으키는 것으로 보여진다.In addition, harmful bacteria destroy nutrients in the intestine and break down vitamins, causing an imbalance in nutritional supply.Because the antibacterial substances produced by Bacillus polypermanticus kill these harmful bacteria, the body absorbs nutrition. Because Permenticus synthesizes vitamins and secretes digestive enzymes to show digestion, it increases the efficiency of nutrition and eventually increases egg production.

또한 직접적으로는 유해균을 박멸하므로 인하여 유해균으로 인한 동물에서의 폐사율이 현저히 개선되는 것으로 인식 될 수 있다. 출산율은 영양부족과 질병감염으로 인한 유산율을 낮추므로 인하여 증가하는 것으로 볼 수 있다.It can also be recognized that mortality in animals due to harmful bacteria is significantly improved due to the direct elimination of harmful bacteria. Fertility rates can be seen to increase due to lower rates of miscarriage due to malnutrition and disease infection.

본 발명의 목적은 바실러스 폴리퍼멘티쿠스를 동물용 사료첨가제로 사용함으로서 출산율 증가시키고, 유해균을 죽여 폐사율을 개선하고, 산란율을 높여 농가소득에 기여할 수 있도록 한 바실러스 폴리퍼멘티쿠스를 함유한 동물용 사료를 제공함에 있다.It is an object of the present invention to increase the fertility rate by using Bacillus polypermanticus as an animal feed additive, to improve the mortality by killing harmful bacteria, and to increase the spawning rate for animals containing Bacillus polypermanticus In providing feed.

도1은 바실러스 폴리퍼멘티쿠스가 생성하는 항균성 물질로 인하여 사멸된 유해균을 관찰한 대표 실험 자료로써, 바실러스 폴리퍼멘티쿠스를 배지에서 키운 후 배지를 뒤집어 반대편에 유해균을 생육시켜 그 생육 억제 현상을 관찰한 사진1 is a representative experimental data observing the harmful bacteria killed by the antimicrobial material produced by Bacillus polypermanticus, after growing the Bacillus polypermanticus in the medium and inverting the medium to grow harmful bacteria on the other side to suppress the growth phenomenon Photograph

본 발명은 바이넥스 연구소가 보유중인 종균인 바실러스 폴리퍼멘티쿠스를 동물용 동물사료에 첨가하여 장내 유해균을 억제 시키고 소화능력을 향상 시키며 영양을 보급하고 또한 항생제 투여로 인한 장내 균총의 파괴를 방지할 용도로 사용코자 하며 그 결과로 출산율 증가, 산란율 증가 및 폐사율 개선을 보여주었다.The present invention is added to the animal feed of Bacillus polyperienticus spawned by BINEX R & D to suppress the intestinal harmful bacteria, improve digestive capacity, supply nutrients, and also prevent the destruction of the intestinal flora by antibiotic administration The results showed an increase in fertility, spawning and mortality.

이와 같은 결과를 가져오는 본 발명의 바실러스 폴리퍼멘티쿠스를 함유한 동물용 사료는 동물의 산란율 증가, 출산율 증가, 폐사율 감소 효과를 위하여 동물사료에 바실러스 폴리퍼멘티쿠스(Bacillus polyfermenticus) 성분을 함유시켜 된 것이고, 상기 바실러스 폴리퍼멘티쿠스 성분의 함유량은 동물사료 1톤당 바실러스 폴리퍼멘티쿠스를 1 ×109개(1 ×109cfu)∼ 5 ×1010cfu 이다.Animal feed containing Bacillus polypermenticus of the present invention that results in such a result by containing Bacillus polyfermenticus component in the animal feed for the effect of increasing the egg laying rate, fertility rate, and mortality of the animal The content of the Bacillus polyfermentus component is 1 × 10 9 Bacillus polyfermentus (1 × 10 9 cfu) to 5 × 10 10 cfu per ton of animal feed.

이하, 본 발명의 실시 예(실제 실험 예)를 들어 상세히 설명하면 다음과 같다.Hereinafter, the embodiment (actual experiment example) of the present invention will be described in detail as follows.

본 발명의 실시 예에서는 동물사료 첨가제로서의 바실러스 폴리퍼멘티쿠스를 복용시킨 복용법과 복용량을 나타내었고, 그에 따른 결과를 보여주고 있다. 이 결과는 부산에 위치한 동양축산에 의뢰하여 얻은 결과로서 부산 경남 지역의 농장에서 실시한 결과를 예시한 것이다.In the embodiment of the present invention was shown the dosage and dosage of Bacillus polypermanticus as an animal feed additive, and shows the results accordingly. This result is a result obtained by commissioning from Dongyang Livestock, located in Busan, and illustrates the results from farms in Gyeongnam, Busan.

[실시예 1]Example 1

바실러스 폴리퍼멘티쿠스(상품명 : 바이넥스 뉴비스 )를 시중에서 판매되는 동물사료에 일정량 첨가하여 가축에 복용 시켰다, 바이넥스 뉴비스에는 1킬로그램(1kg)중에 다음과 같이 함유되어 있다.Bacillus polyfermentus (trade name: Binex Nubis) was added to a commercial animal feed in a certain amount and was taken to livestock, and Binex Nubis contained 1 kg (1 kg) as follows.

생균으로서 바실러스 폴리퍼멘티쿠스 백억마리(1 ×1010cfu)를 주성분으로 하여 제조하였고 여기에 유산균(streptococcus faecalis) 70억 마리( 7 ×109cfu), 낙산균 2억마리( 2 ×108cfu)를 첨가하였고 추가적으로 판크레아틴 0.1 배산(소화력의 역가를 나타내는 단위, 1배산은 배당체 1마이크로 당량을 1분동안에 가수분해시키는 속도로 기질을 분해시키는데 필요한 판크레아틴 효소의 량)과 부형제를 사용하여 총 1킬로그램을 만들어서 공급하고 이 제제를 시중에서 판매되는 사료 1톤(1ton)당 1.0kg을 첨가하여 산란계에 복용시켰고, 그 결과를 하기 표1 및 표2에 나타내었다.Bacillus polyfermenticus 10 billion (1 × 10 10 cfu) as a main component was produced as live bacteria, 7 billion (7 × 10 9 cfu) lactic acid bacteria (7 × 10 9 cfu), 200 million (2 × 10 8 cfu) And additionally using pancreatin 0.1 fold (the unit representing the potency of the digestive power, 1 fold is the amount of pancreatin enzyme required to break down the substrate at a rate that hydrolyzes 1 microgram of glycoside in 1 minute) and excipients One kilogram was made and supplied, and the preparation was added to a laying hen with 1.0 kg per ton (1 ton) of commercially available feed, and the results are shown in Tables 1 and 2 below.

먼저 하기 표1은 22만 1천마리의 산란계에 대한 복용 결과로, 폐사율과 평균 산란율을 측정하였다. 그리고 복용량은 시중에서 판매하는 동물용 사료 1톤당 바실러스 폴리퍼멘티쿠스를 1 ×1010개(1 ×1010cfu)를 함유하도록 하여 복용시킨 결과이다.Table 1 below shows the results of doses for 221,000 laying hens, and measured mortality and average laying rate. And the dose is a result of taking, to contain the animal feed per ton of Bacillus poly flops mentee kusu for a commercially available 1 × 10 10 gae (1 × 10 10 cfu).

그 결과 하기 표1과 같이 본 발명에 따른 사료를 복용하기 전의 폐사율이 19.5%인데 비하여 복용 후의 폐사율은 1.98%를 보여 17.52%의 폐사를 줄였고, 평균 산란율도 복용 전의 산란율이 76.0%에서 90.96%로 개선되어 14.96% 증가된 산란율을 보여 주었다. 또한 산란율 90% 이상을 유지한 일수도 연속적으로 16주를 보이는 효과를 나타내었다.As a result, as shown in Table 1, the mortality rate before taking the feed according to the present invention was 19.5%, while the mortality rate after taking the meal was 1.98%, which reduced 17.52% mortality, and the average egg laying rate was 76.0% to 90.96%. It improved, showing an increase of 14.96% spawning rate. In addition, the number of days maintaining more than 90% of the spawning rate also showed the effect of 16 weeks in a row.

[표 1]TABLE 1

하기 표2는 약 22만 마리 닭에 시중에서 판매하는 사료에 사료첨가제로 바실러스 폴리퍼멘티쿠스(Bacillus polyfermenticus)를 첨가한 사료를 동물에 복용시킴으로서 주령에 따른 산란수, 산란율(90.96%)과 폐사율(1.98%) 결과 이다.Table 2 shows the number of laying eggs, spawning rate (90.96%) and mortality according to age by taking the feed of Bacillus polyfermenticus added as a feed additive to a commercially available feed to about 220,000 chickens. 1.98%) result.

[표 2]TABLE 2

[실시예 2]Example 2

바실러스 폴리퍼멘티쿠스 (상품명 : 바이넥스 뉴비스)를 사료에 일정량 첨가하여 가축에 복용시켰다. 시중에서 판매되는 사료 1톤당 바이넥스 뉴비스 330그램을 첨가하여 20만 4천마리의 산란계에 대한 복용 결과로 복용전의 폐사율이 14.5%인데 비하여 복용 후 2.2%를 보여 12.3%의 폐사를 줄였고 평균 산란율이 77.7%에서 83.9%로 개선되어 6.2%증가된 산란율을 보여 주었다. 또한 산란율 90% 이상을 유지한 일수도 연속적으로 16주를 보이는 효과를 나타내었다.(표 3 참조)Bacillus polyfermenticus (trade name: Vinex Nubis) was added to the feed in an amount to be taken to livestock. By adding 330 grams of Binex Newis per tonne of commercial feed, 204,000 laying hens resulted in a 14.5% mortality rate compared to 14.5%, reducing 12.3% mortality and an average laying rate of 77.7. It was improved from 8% to 83.9%, showing an increase of 6.2%. In addition, the number of days maintaining more than 90% of the spawning rate also showed 16 weeks continuous effect (see Table 3).

[표 3]TABLE 3

하기 표4는 20만4천 마리 닭에 시중에서 판매하는 사료에 사료첨가제로 바실러스 폴리퍼멘티쿠스(Bacillus polyfermenticus)를 첨가한 사료를 동물에 복용시킴으로서 주령에 따른 산란수, 산란율(83.9%)과 폐사율(2.2%) 결과 이다.Table 4 shows the spawning water, laying rate (83.9%) and mortality according to age by ingesting a feed containing Bacillus polyfermenticus as a feed additive to a commercially available feed for 204,000 chickens . (2.2%) is the result.

[표 4]TABLE 4

[실시예 3]Example 3

모돈에 사료 1톤(1ton)당 바실러스 폴리퍼멘티쿠스(상품명 : 바이넥스 뉴비스 )를 1.0kg을 첨가하여 복용시켰다. 그 결과 출산수가 10마리에서 12-14마리로 증가됨을 보여주었다.The sows were dosed by adding 1.0 kg of Bacillus polyfermenticus (trade name: Vinex Nubis) per ton of feed. The results showed that the number of births increased from 10 to 12-14.

한편 본 발명에 따른 도 1은 바실러스 폴리퍼멘티쿠스가 생성하는 항균성 물질로 인하여 사멸된 유해균을 관찰한 대표 실험 자료로써, 바실러스 폴리퍼멘티쿠스를 배지에서 키운 후 배지를 뒤집어 반대편에 유해균을 생육시켜 그 생육 억제 현상을 관찰한 것이다.(Flip plate method)Meanwhile, FIG. 1 according to the present invention is a representative experimental data for observing harmful bacteria killed by the antimicrobial material produced by Bacillus polyfermentus, grows Bacillus polyfermentus in the medium and then inverts the medium to grow harmful bacteria on the other side. The growth inhibition phenomenon was observed. (Flip plate method)

이것은 바실러스 폴리퍼멘티쿠스가 생성하는 항균성 물질(박테리오신, 젓산, 초산)로 인하여 사멸된 유해균을 관찰한 것으로써 중앙부위의 괴사되어 검게 보이는 모양이 유해균이 억제됨을 표시하며, 황국균과 흑국균의 경우에 비스루트 접종시에 둥근 원형이 억제되어 생육 저해됨을 보이고 있다.This was observed by the harmful bacteria killed by the antimicrobial substances (bacteriocin, lactic acid, acetic acid) produced by Bacillus polypermanticus, which indicates that the necrotic and black shapes of the central area are suppressed. It has been shown that the round circle is inhibited at the time of inoculation of bisruit, which inhibits growth.

Flip plate method에 의한 항균효과가 검정된 종은 다음과 같다.Species tested for antimicrobial effect by Flip plate method are as follows.

Bacillus cereusATCC 11778 Bacillus cereus ATCC 11778

Pseudomonas aeruginosaATCC 15442 Pseudomonas aeruginosa ATCC 15442

Salmonella enteritidis&paratyphiA Salmonella enteritidis & paratyphi A

Shigella flexneri , boydii&sonnei Shigella flexneri, boydii & sonnei

Vibrio cholerae 0139&parahaemolyticusATCC17802 Vibrio cholerae 0139 & parahaemolyticus ATCC17802

Yersinia enterocolitica& Yersinia enterocolitica &

pseudotuberculosisATCC 6902 pseudotuberculosis ATCC 6902

Staphylococcus aureusATCC 28923 Staphylococcus aureus ATCC 28923

Pseudomonas syringaeATCC 12855 Pseudomonas syringae ATCC 12855

E. coli ATCC29522 & ATCC 8739 E. coli ATCC 29522 & ATCC 8739

Aeromonas hydrophilaAeromonas hydrophila

Listeria ivanoviiATCC 19119 Listeria ivanovii ATCC 19119

Micrococcus luteusATCC 10240 Micrococcus luteus ATCC 10240

Bacillus subtilisATCC 6633 Bacillus subtilis ATCC 6633

Enterococcus faecalisATCC 19433 Enterococcus faecalis ATCC 19433

상기한 구성의 본 발명에 따르면, 바실러스 폴리퍼멘티쿠스가 첨가된 사료를 동물에 복용시킴으로서 폐사율 개선, 출산율 증가, 산란율 증가를 나타내었다. 상기에서 확인 할 수 있는 바와 같이 바실러스 폴리퍼멘티쿠스를 복용시킴으로서 농가소득 증대에 기여 할 수 있음을 보여주고 있다.According to the present invention having the above-described configuration, by taking an animal to which the Bacillus polypermanticus is added to the animal showed an improvement in mortality, increased fertility, and increased egg production. As can be seen above, taking Bacillus polypermanticus has been shown to contribute to increase in farm household income.

Claims (2)

동물의 산란율 증가, 출산율 증가, 폐사율 감소 효과를 위하여 동물사료에 바실러스 폴리퍼멘티쿠스(Bacillus polyfermenticus), 스트렙토코쿠스 피칼리스(Streptococcus faecalis), 낙산균, 판크레아틴 및 부형제를 함유시켜 된 것을 특징으로 하는 바실러스 폴리퍼멘티쿠스를 함유한 동물용 사료.Animal feed contains Bacillus polyfermenticus , Streptococcus faecalis , lactic acid bacteria , pancreatin, and excipients for increased egg production, fertility, and mortality. Animal feed containing Bacillus polyfermentus. 제1항에 있어서, 동물사료 1톤당 상기 바실러스 폴리퍼멘티쿠스 의 함유량은 1 ×1010cfu, 상기 스트렙토코쿠스 피칼리스의 함유량은 7×10cfu, 상기 낙산균의 함유량은 2×10cfu, 상기 판크레아틴의 함유량은 0.1배산임을 특징으로 하는 바실러스 폴리퍼멘티쿠스를 함유한 동물용 사료.The method of claim 1, wherein the content of the Bacillus polypermanticus per tonne of animal feed is 1 × 10 10 cfu, the content of the Streptococcus picalis is 7 × 10 cfu, the content of the lactic acid bacteria is 2 × 10 cfu, the animal feed containing Bacillus polyfermenticus, characterized in that the content of the pancreatin is 0.1-fold.
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