KR100441741B1 - Improved methods of mycelial culture of Phellinus linteus KCTC 10055BP for increasing anti-oxidanic effects - Google Patents

Improved methods of mycelial culture of Phellinus linteus KCTC 10055BP for increasing anti-oxidanic effects Download PDF

Info

Publication number
KR100441741B1
KR100441741B1 KR10-2001-0055045A KR20010055045A KR100441741B1 KR 100441741 B1 KR100441741 B1 KR 100441741B1 KR 20010055045 A KR20010055045 A KR 20010055045A KR 100441741 B1 KR100441741 B1 KR 100441741B1
Authority
KR
South Korea
Prior art keywords
mushroom mycelium
mycelium
medium
hawthorn
antioxidant
Prior art date
Application number
KR10-2001-0055045A
Other languages
Korean (ko)
Other versions
KR20030021673A (en
Inventor
한영환
오정석
김주영
김성준
Original Assignee
한영환
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 한영환 filed Critical 한영환
Priority to KR10-2001-0055045A priority Critical patent/KR100441741B1/en
Publication of KR20030021673A publication Critical patent/KR20030021673A/en
Application granted granted Critical
Publication of KR100441741B1 publication Critical patent/KR100441741B1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00—Cultivation of mushrooms
    • A01G18/10—Mycorrhiza; Mycorrhizal associations

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Mycology (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Botany (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Environmental Sciences (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

본 발명은 새로운 상황버섯 균사체 제조법에 관한 것으로, 특히 항산화 및 노화방지 효과를 크게 증대시킬 수 있는 상황버섯 균사체 제조법에 관한 것이다. 이를 위해 본 발명은 여러 천연물 중에서 산사 및 산사육 추출물을 기존의 상황버섯 균사체 배양용 배지(YMG)에 첨가하여 새롭게 배지를 제조한다. 제조된 배지를 고온고압 습윤멸균 시킨 후 상황버섯 균사체를 접종하여 배양한다. 배양된 상황버섯 균사체 추출물의 리보플라빈과 빛에 의한 산화·환원시 생성되는 수퍼옥사이드 라디칼에 대해서 증가된 항산화 및 노화방지 효과에 대한 방법을 제공한다.The present invention relates to a new situation mushroom mycelium production method, and more particularly to a method for producing a situation mushroom mycelium that can greatly increase the antioxidant and anti-aging effect. To this end, the present invention is added to the hawthorn and hawthorn extract from the existing natural mushroom mycelium culture medium (YMG) of several natural products to prepare a new medium. After the high temperature and high pressure wet sterilization of the prepared medium was inoculated with the mushroom mycelium cultured. The present invention provides a method for increased antioxidant and anti-aging effects on riboflavin and superoxide radicals produced during oxidation and reduction by light of cultured mushroom mycelium mycelium.

Description

항산화 효과를 증진시키는 상황버섯의 균사체 제조법{Improved methods of mycelial culture of Phellinus linteus KCTC 10055BP for increasing anti-oxidanic effects}Improved method of mycelial culture of Phellinus linteus KCTC 10055BP for increasing anti-oxidanic effects

본 발명은 상황버섯 균사체 배양물의 항산화 및 노화방지 효과를 상승시키기 위하여 천연물 을 이용하여 증강된 항산화 효과가 있는 상황버섯 균사체 제조에 그 목적을 둔다.The present invention aims to produce a situation mushroom mycelium having an enhanced antioxidant effect using natural products to increase the antioxidant and anti-aging effect of the situation mushroom mycelium culture.

상황버섯의 새로운 기능성New functionality of the situation mushroom

상황버섯(Phellinus linteus)은 대표적인 약용버섯 중의 하나로 항보체 활성(송치현 외 1998균학회지26:86-90), B-임파구 활성(Song et. al. 1995.Chem. Pharm. Bull.43:2105-2108),Phellinus linteus (Phellinus linteus) is one of the representative medicinal mushrooms. Anti-complement activity (Song Chi-hyun et al. 1998: 26-86-90), B-lymphocyte activity (Song et. Al. 1995. Chem. Pharm. 2108),

체액 및 세포성 면역 활성(Kim et. al. 1996.Int. J. Immunopharmac. 18:295-303), 암 전이 방지효과(Han et. al. 1999.Immunopharmacol. 41:157-164) 등 상황버섯 균사체 및 균사배양액으로부터 생산된 다당체의 면역증강 및 암의 예방에 관련된 연구들이 주를 이뤄왔다. 최근 상황버섯의 다양한 기능성에 관한 연구가 진행되어 항혈당 효과(Kim et. al. 2001.J. Microbiol. Biotechnol. 11:167-171) 및 항산화 효과(특허출원번호 10-2000-0054321)등 면역증강 효과 이외의 다양한 기능성이 밝혀졌다.Humoral and cellular immune activity (Kim et. Al. 1996. Int. J. Immunopharmac. 18: 295-303), cancer metastasis prevention effect (Han et. Al. 1999. Immunopharmacol. 41: 157-164) Research has been focused on the immunity enhancement and the prevention of cancer of polysaccharides produced from mycelia and mycelium broth. Recently, studies on the various functionalities of the situation mushroom have been conducted, such as anti-glycemic effect (Kim et. Al. 2001. J. Microbiol. Biotechnol. 11: 167-171) and antioxidant effect (Patent application No. 10-2000-0054321) Various functionalities other than enhancement effects have been found.

항산화 효과Antioxidative effect

생명유지에 절대적으로 필요한 산소가 체내 효소계, 환원대사, 화학약품, 공해물질, 광화학반응 등 각종 물리적 화학적요인에 의해 반응성이 매우 큰 활성산소(수퍼옥사이드 라디칼, 하이드록실 라디칼 및 과산화 수소)로 전환되는데 이것이 생체에 치명적인 산소독성을 일으킨다. 즉 활성산소는 세포구성 성분들인 지질, 단백질, 당, DNA 등에 파괴작용을 함으로써, 암을 비롯하여 각종 질환과 노화를 일으키는 것으로 알려져 있는데 이러한 활성산소를 소거하는 기작이 항산화 활성이다. 지금까지 개발되고 사용되고 있는 항산화제로는 토코페롤, 카로틴, 탄닌 등이 있으며, 천연물 추출물에는 산사 등이 알려져 있다. 이렇듯 개별적으로 보유하고 있는 항산화 및 노화방지효과를 생물공학적 배양법을 이용한다면 항산화 상승효과를 얻을 수 있는데 본 기술의 의의를 둔다Oxygen, which is absolutely necessary for life support, is converted into highly reactive reactive oxygen (superoxide radical, hydroxyl radical and hydrogen peroxide) by various physical chemical factors such as enzyme system, reducing metabolism, chemicals, pollutants and photochemical reaction. This causes deadly oxygen toxicity to the living body. That is, active oxygen is known to cause various diseases and aging including cancer by destroying lipids, proteins, sugars, DNA, and the like, which is an antioxidant activity. Antioxidants that have been developed and used so far include tocopherol, carotene, tannin, and the like. The anti-oxidation and anti-aging effects of these individual compounds can be obtained by using biotechnological cultivation methods.

본 발명자들은 상황버섯이 가지고 있는 항산화 및 노화방지능력을 배가시키기 위해 여러 가지 천연물 추출액을 대상으로 하여 연구하였다. 이 중 산사와 산사육를 배지에 첨가하여 상황버섯 균사체을 배양한 결과 각각 독립적인 형태에 비해 최대 21배의 항산화 및 노화방지 효과의 증가를 나타냈기에 본 발명을 완성하게 되었다.The present inventors studied various natural product extracts to double the antioxidant and anti-aging ability of the situation mushroom. Among them, the cultivated mycelium mushroom mycelium was added to the medium and mountain cultivation medium to increase the antioxidant and anti-aging effect of up to 21 times as compared to the independent form was completed the present invention.

본 발명에 사용되는 상황버섯 균사체는 발명자들이 경주지역에서 분리하여 배양하고 부산대학교 미생물학과에 의뢰하여Phellinus linteus로 동정 받았으며(정지원 등. 1999.Kor. J. Mycol. 27:124-131) 생명공학연구소에 유전자은행(KCTC)에 기탁한Phellinus linteusThe situation mushroom mycelium used in the present invention was isolated and cultured in the Gyeongju area by the inventors and was identified as Shellinus linteus by the Department of Microbiology at Pusan National University (Jeong Ji-won et al. 1999. Kor. J. Mycol. 27: 124-131) Shellinus linteus deposited with KCTC at the Institute

본 발명은 발명자들이 분리한 상황버섯Phellinus linteusKCTC 10055BP가 산사 및 산사육이 첨가된 새로운 배지에서 나타내는 증가된 항산화 및 노화방지 효능에 관한 것이다.The present invention relates to the increased antioxidant and anti-aging efficacy of the Phellinus linteus KCTC 10055BP isolated from the inventors in a new medium supplemented with hawthorn and hawthorn.

[실시예 1]본 발명의 배지에 첨가되는 산사 및 산사육는 일반 한약상에서 건조체를 입수하여 사용하였다. 발명에 사용할 산사 및 산사육 추출물을 얻기 위해 건조된 산사 및 산사육 150g을 추출병에 넣고 증류스를 부어 80 ~ 121℃의 온도로 5시간 3회 정도 추출하였다. 이렇게 제조된 추출물은 여과지로 걸러 여과한 후, 감압농축기를 이용하여 10배 감압 농축하여 농축액을 제조하였다. 이를 24시간 냉동시킨 후 동결건조기를 이용하여 시료분말을 제조하였다[Example 1] The hawthorn and lean breeding material added to the medium of the present invention were obtained by using a dry body in general Chinese medicine. In order to obtain the hawthorn and hawthorn extract to be used in the invention, 150 g of dried hawthorn and hawthorn was put in an extraction bottle, and distilled, and extracted three times for 5 hours at a temperature of 80 to 121 ° C. The extract thus prepared was filtered through a filter paper, and then concentrated under reduced pressure using a vacuum condenser 10 times to prepare a concentrate. After freezing for 24 hours, a sample powder was prepared using a lyophilizer.

[실시예 2]상황버섯 균사세 배양에 사용된 배지는 포도당 0.4%, 효모추출물 0.4%, 맥아 추출물 1.0%를 첨가하여 제조 하였으며, 미리 준비된 산사 및 산사육 시료분말 0.1%를 첨가하여 121℃, 1.5기압하에서 20분간 멸균 후, 전배양된 상황버섯 균사체 종균을 5%(v/v) 되게 접종 후, 24℃에서 15일간 액체 심부 배양한다.[실시예 3]본 발명에 사용할 샘플을 준비하기위해 배양된 상황버섯 균사체는 균질기(Homogenizer)를 이용하여 약 5분간 균질화 한 후 원심분리하여 상등액을 취한다. 얻어진 상등액으로 실험예 1의 항산화 및 노화방지작용 시험을 수행하였으며, 수행한 샘플의 종류는 표 1과 같다.[Example 2] The medium used for the cultivation of the mushroom mushroom mycelium was prepared by adding glucose 0.4%, yeast extract 0.4%, and malt extract 1.0%. After sterilization under atmospheric pressure for 20 minutes, the precultured mycelial mushroom mycelium spawn was inoculated at 5% (v / v), and then liquid deep culture was performed at 24 ° C. for 15 days. [Example 3] The cultured mushroom mycelium is homogenized for about 5 minutes using a homogenizer and centrifuged to take a supernatant. The antioxidant and anti-aging test of Experimental Example 1 was carried out with the obtained supernatant, and the types of samples carried out are shown in Table 1 below.

표 1. 항산화 및 노화방지 상승효과를 위한 시료 시료명 조건 PL-C 상황버섯 균사체 PL-CP01 산사 추출물이 첨가 된 배지에서 생장한 상황버섯 균사체 PL-CP02 산사육 추출물이 첨가된 배지에서 생장한 상황버섯 균사체 CP01 산사 추출물 CP02 산사육 추출물 Table 1. Samples for Antioxidant and Anti-aging Synergy Sample name Condition PL-C Situation Mushroom Mycelium PL-CP01 Situation Mushroom Mycelium Growing in Medium Added Sansa Extract PL-CP02 Situation Mushroom Mycelium Growing in Medium Added with Livestock Extract CP01 Hawthorn extract CP02 Mountain Breeding Extract

[실험예 1]항산화 효과 검정 시험Experimental Example 1 Antioxidant Effect Assay

본 발명의 항산화 효과 검정 시험은 Winterbourn(1975.J. Lab. Clin. Med.85:337)의 방법에 준하여 실시하였다. 3 ㎖ 시험관에 0.1 M EDTA(0.3 mM cyanide 포함) 0.2 ㎖, 1.5 mM NBT 0.1 ㎖, 40 mM Tris 완충액(buffer) 2.6 ㎖ 및 샘플 0.1 ㎖을 넣고 약 5분간 안정화 시킨 다음 0.12 mM 리보플라빈 0.05 ㎖을 넣고 1,000 lux의 백열등을 조사한다. 1분에 1번씩 560 ㎚에서 흡광도 값을 10회 측정하고 평균값을 구하여 1분간 변화하는 흡광도 값을 구한다. 이에 이용한 공식은 다음과 같으며, 실험결과는 표 2에 나타낸 바와 같다.The antioxidant effect assay test of the present invention was carried out according to the method of Winterbourn (1975. J. Lab. Clin. Med. 85: 337). Add 0.2 ml of 0.1 M EDTA (containing 0.3 mM cyanide), 0.1 ml of 1.5 mM NBT, 2.6 ml of 40 mM Tris buffer and 0.1 ml of sample in a 3 ml test tube, stabilize for about 5 minutes, add 0.05 ml of 0.12 mM riboflavin Incandescent lamp of 1,000 lux. Once per minute, the absorbance value is measured 10 times at 560 nm, and the average value is obtained to determine the absorbance value that changes for 1 minute. The formula used is as follows, and the experimental results are shown in Table 2.

Unit = (대조군의 1분간 Δ흡광도 560 ㎚ /시료의 1분간 Δ흡광도 560 ㎚)-1[표 2] 각각의 시료의 농도별 항산화 효과 농도별 항산화 효과(Unit) 0.01 0.1 0.5 1 2.0 PLC 0.06 2.8 3.9 4.5 5 PL-CP01 3.1 8.8 9.6 11.5 12.2 PL-CP02 2.5 6.5 8.8 10.5 11 CP01 0.5 3 4 5.2 5.4 CP02 0.2 1.5 1.8 2.5 3.1 Unit = (Δ absorbance 560 nm for 1 minute of control / Δ absorbance 560 nm for 1 minute of sample) -1 [Table 2] Antioxidant effect of each sample concentration Antioxidant Effect by Concentration (Unit) 0.01 0.1 0.5 One 2.0 PLC 0.06 2.8 3.9 4.5 5 PL-CP01 3.1 8.8 9.6 11.5 12.2 PL-CP02 2.5 6.5 8.8 10.5 11 CP01 0.5 3 4 5.2 5.4 CP02 0.2 1.5 1.8 2.5 3.1

상기 결과와 같이 실시예 3의 상황 버섯 균사체 배양액 및 산사 및 산사육이 첨가된 상황 버섯 균사체 배양액, 산사 및 산사육 추출물의 농도가 증가할수록 항산화의 효과가 증가 됨을 알 수 있다. 특히 무첨가 상황 균사체, 산사 및 산사육 추출물이 어느정도의 항산화 활성을 나타내나, 산사 및 산사육 추출물이 첨가된 배지에 상황을 배양할 경우 매우 높은 항산화 활성을 나타내었다. 이는 우수한 약리효능을 나타내는 상황을 생산하기 위해 산사 및 산사육을 상황 배양시 첨가할 경우 항산화 활성을 증가시킬수 있는 결과를 나타낸다.As described above, it can be seen that as the concentration of the mushroom mushroom mycelium culture medium and hawthorn mushroom mycelium culture medium, hawthorn and hawthorn extract of Example 3 is increased, the antioxidant effect increases. In particular, the mycelium, hawthorn and hawthorn extract showed no antioxidant activity. However, when the cultivation of the cultivars in the medium to which the hawthorn and hawthorn extract was added showed a very high antioxidant activity. This results in an increase in antioxidant activity when hawthorn and hawthorn are added during culturing to produce a situation that exhibits good pharmacological efficacy.

산사 및 산사육 추출물 과 기존의 방법으로 배양된 상황균사체 추출물에서도 항산화 및 노화방지 효과를 관찰 할 수 있었으나, 산사 및 산사육 추출물이 첨가된 배지에서 생장된 상황버섯 균사체는 최소 3배에서 최대 21배까지 항산화 및 노화방지 상승효과를 관찰 할 수 있었다. 따라서 상황버섯의 항산화 및 노화방지 효과를 극대화시키기 위해서는 산사 및 산사육 추출물을 첨가하는 것이 본 발명의 효과이다.Antioxidant and anti-aging effects were observed in hawthorn and hawthorn extracts and cultivated mycelium mycelium cultured by conventional methods. And anti-aging synergistic effect could be observed. Therefore, in order to maximize the antioxidant and anti-aging effect of the situation mushrooms, the addition of the hawthorn and mountain breeding extract is the effect of the present invention.

Claims (3)

산사 및 산사육 추출물을 상황버섯 균사체 배양시 0.01 ~ 2.0 중량%으로 배지에 첨가하여 균사체 액체배양하는 것을 특징으로 하는 항산화 활성을 증진시키는 상황버섯 균사체 제조법.Sichuan mushroom mycelium production method to enhance the antioxidant activity, characterized in that the mycelium liquid culture is added to the medium at 0.01 ~ 2.0% by weight during cultivation of mushroom mushroom mycelium. 1항의 산사 추출물은 증류수를 용제로 하여 추출되고, 배양시에 사용되는 배지에 0.01 ~ 2.0 중량%으로 배지에 첨가하여 균사체 액체배양하는 상황버섯 균사체 제조법.The pulverulent mushroom mycelium preparation method of extracting the mycelia of 1 paragraph is extracted with distilled water as a solvent and added to the medium at 0.01 to 2.0% by weight to the medium used in the culture. 1항의 산사육 추출물은 증류수를 용제로 하여 추출되고, 배양시에 사용되는 배지에 0.01 ~ 2.0 중량%으로 배지에 첨가하여 균사체 액체배양하는 상황버섯 균사체 제조법.The cultivation of mycelium mushroom mycelium 1 is extracted with distilled water as a solvent and added to the medium at 0.01 to 2.0% by weight to the medium used in the culture.
KR10-2001-0055045A 2001-09-07 2001-09-07 Improved methods of mycelial culture of Phellinus linteus KCTC 10055BP for increasing anti-oxidanic effects KR100441741B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR10-2001-0055045A KR100441741B1 (en) 2001-09-07 2001-09-07 Improved methods of mycelial culture of Phellinus linteus KCTC 10055BP for increasing anti-oxidanic effects

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR10-2001-0055045A KR100441741B1 (en) 2001-09-07 2001-09-07 Improved methods of mycelial culture of Phellinus linteus KCTC 10055BP for increasing anti-oxidanic effects

Publications (2)

Publication Number Publication Date
KR20030021673A KR20030021673A (en) 2003-03-15
KR100441741B1 true KR100441741B1 (en) 2004-07-23

Family

ID=27722986

Family Applications (1)

Application Number Title Priority Date Filing Date
KR10-2001-0055045A KR100441741B1 (en) 2001-09-07 2001-09-07 Improved methods of mycelial culture of Phellinus linteus KCTC 10055BP for increasing anti-oxidanic effects

Country Status (1)

Country Link
KR (1) KR100441741B1 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101855421B1 (en) * 2014-10-13 2018-07-20 농업회사법인자연생명대체의학(주) Agricultural products with a pharmacological action and the mycelium mixtures are fermented food composition method of preparing the same
KR101599699B1 (en) * 2015-09-15 2016-03-04 주식회사 일심바이오 Method for manufacturing culture medium of sparassis crispa using antioxidantm, amino acid and vitamin
KR101599697B1 (en) * 2015-09-15 2016-03-04 주식회사 일심바이오 Method for manufacturing culture medium of sparassis crispa using antioxidant and amino acid

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995003816A1 (en) * 1993-07-29 1995-02-09 Willmar Schwabe Gmbh & Co. Compositions of crataegus species, medicaments and their use for preventing sudden death due to cardiac arrest and reperfusion-caused cardiovascular lesions
KR0152692B1 (en) * 1994-10-27 1998-09-15 안영남 Method of cultivating phellinus linteus
KR20000045576A (en) * 1998-12-30 2000-07-25 유상옥 Cosmetic composition containing the extract of crataegi fructus useful for removing free radicals from the skin
KR100338318B1 (en) * 1999-09-15 2002-05-27 김천환 Culturing method of cordyceps spp

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995003816A1 (en) * 1993-07-29 1995-02-09 Willmar Schwabe Gmbh & Co. Compositions of crataegus species, medicaments and their use for preventing sudden death due to cardiac arrest and reperfusion-caused cardiovascular lesions
KR0152692B1 (en) * 1994-10-27 1998-09-15 안영남 Method of cultivating phellinus linteus
KR20000045576A (en) * 1998-12-30 2000-07-25 유상옥 Cosmetic composition containing the extract of crataegi fructus useful for removing free radicals from the skin
KR100338318B1 (en) * 1999-09-15 2002-05-27 김천환 Culturing method of cordyceps spp

Also Published As

Publication number Publication date
KR20030021673A (en) 2003-03-15

Similar Documents

Publication Publication Date Title
CN109939027B (en) Method for preparing ergothioneine-containing cosmetic stock solution by fermenting hericium erinaceus
KR101467903B1 (en) The preparing method of immune improving agents
CN103156052A (en) Submerged fermentation technology of producing immunological enhancement active materials of inonotus obliquus
CN101702984B (en) Phellinus igniarius mycelium liquid fermentation method by utilizing fungal elicitor for improving yield of flavone of phellinus igniarius
KR20210118391A (en) Ergothioneine-producing strain and method for selection thereof
JP6560301B2 (en) Method for promoting laccase production by microorganisms
CN112501029B (en) Armillaria matsutake and method for producing ergothioneine by using same
Choi et al. Enhancement of anti-complementary and radical scavenging activities in the submerged culture of Cordyceps sinensis by addition of citrus peel
US20060078971A1 (en) Isoflavone-beta-D-glucan produced by Agaricus blazei in the submerged liquid culture and method of producing same
KR101612421B1 (en) Novel Yeast Overproducing Glutathione and Method for Glutathione Overproduction by Using it
JP5850497B2 (en) Method for producing and purifying cordycepin
KR102216605B1 (en) Method for increasing production of luteolin and luteolin-7-glucoside using microbial fermentation
KR100441741B1 (en) Improved methods of mycelial culture of Phellinus linteus KCTC 10055BP for increasing anti-oxidanic effects
US20040106187A1 (en) Composition for the culturing of Phellinus linteus mycelium
KR100491362B1 (en) Method for preparing immune enhancing polysaccharides
CN113308378A (en) Ganoderma lucidum strain for high-yield ergothioneine and application thereof
KR100706132B1 (en) Composition for the culturing of Phellinus linteus mycelium
CN115491317A (en) Flavonoid compound production strain and application thereof
KR100597950B1 (en) Method of prepareing antioxidant by using mushroom mycelium
CN100389190C (en) Antrodia mycelium active substance preparation method and its components
KR100754821B1 (en) Method of mass production for mycelia and extracellular polysaccharides of the same cordyceps sphecocephala
KR100218897B1 (en) Medium for phellinus linteus culture and culturing method of phellinus linteus with the medium
SASKIAWAN Exopolysaccharide production and its bioactivities of the edible Pleurotus ostreatus in submerged culture
KR20170114603A (en) Mask pack sheet of Ceriporia lacerata and the method for preparing thereof
KR100393254B1 (en) A culture medium for Phellinus linteus and a culture method using thereof

Legal Events

Date Code Title Description
A201 Request for examination
E902 Notification of reason for refusal
E701 Decision to grant or registration of patent right
GRNT Written decision to grant
FPAY Annual fee payment

Payment date: 20080715

Year of fee payment: 5

LAPS Lapse due to unpaid annual fee