KR100440097B1 - 나트륨/프로톤 대향수송체, 그 수송체를 코드하는 dna, 그 수송체의 제조방법 및 그 dna를 보유하는 식물체 - Google Patents
나트륨/프로톤 대향수송체, 그 수송체를 코드하는 dna, 그 수송체의 제조방법 및 그 dna를 보유하는 식물체 Download PDFInfo
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- KR100440097B1 KR100440097B1 KR10-2001-7007836A KR20017007836A KR100440097B1 KR 100440097 B1 KR100440097 B1 KR 100440097B1 KR 20017007836 A KR20017007836 A KR 20017007836A KR 100440097 B1 KR100440097 B1 KR 100440097B1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
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- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
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- Biochemistry (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
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- Gastroenterology & Hepatology (AREA)
- Botany (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
Abstract
Description
Claims (30)
- 서열번호: 2 기재의 아미노산서열로 된 단백질을 코드하는 DNA.
- 서열번호: 1 기재의 염기서열의 코드영역을 포함하는 DNA.
- 서열번호: 1 기재의 염기서열로 된 DNA와, 5x SSPE, 2x 덴하드 용액, 0.1%(w/v) SDS, 및 청어 정자 DNA 100 ㎍/ml를 포함하는 하이브리다이제이션 용액 중 25℃에서 하이브리다이즈를 행하고, 2x SSC, 0.1% SDS 중에서 25℃에서 세정하는 조건하에서 하이브리다이즈하는 DNA로서, Na+/H+대향수송체 활성을 갖는 단자엽식물 유래의 단백질을 코드하는 DNA.
- 서열번호: 1 기재의 염기서열로 된 DNA와, 50% 포름아미드, 5x SSPE, 2x 덴하드 용액, 0.1%(w/v) SDS, 및 청어 정자 DNA 100 ㎍/ml를 포함하는 하이브리다이제이션 용액 중 42℃에서 하이브리다이즈를 행하고, 2x SSC, 0.1% SDS 중에서 42℃에서 세정하는 조건하에서 하이브리다이즈하는 DNA로서, Na+/H+대향수송체 활성을 갖는 단자엽식물 유래의 단백질을 코드하는 DNA.
- 서열번호: 1 기재의 염기서열로 된 DNA와, 50% 포름아미드, 5x SSPE, 2x 덴하드 용액, 0.1%(w/v) SDS, 및 청어 정자 DNA 100 ㎍/ml를 포함하는 하이브리다이제이션 용액 중 42℃에서 하이브리다이즈를 행하고, 0.1x SSC, 0.1% SDS 중에서 56℃에서 세정하는 조건하에서 하이브리다이즈하는 DNA로서, Na+/H+대향수송체 활성을 갖는 단자엽식물 유래의 단백질을 코드하는 DNA.
- 제3항 내지 제5항 중 어느 한 항에 있어서, 단자엽식물이 벼과 식물인 DNA.
- 제1항 내지 제5항 중 어느 한 항의 DNA를 포함하는 벡터.
- 제1항 내지 제5항 중 어느 한 항의 DNA를 보유하는 형질전환 세포.
- 제7항의 벡터를 보유하는 형질전환 세포.
- 제8항에 있어서, 식물세포인 형질전환 세포.
- 제9항에 있어서, 식물세포인 형질전환 세포.
- 제1항 내지 제5항 중 어느 한 항의 DNA에 의해 코드되는 단백질.
- 제8항의 형질전환 세포를 배양하여, 상기 세포 또는 그 배양상청으로부터 발현시킨 단백질을 회수하는 공정을 포함하는, 상기 DNA가 코드하는 단백질의 제조방법.
- 제9항의 형질전환 세포를 배양하여, 상기 세포 또는 그 배양상청으로부터 발현시킨 단백질을 회수하는 공정을 포함하는, 상기 DNA가 코드하는 단백질의 제조방법.
- 제10항의 형질전환 세포를 포함하는 형질전환 식물체로서, 칼루스 재분화를 통한 무성생식이 가능한 식물체.
- 제11항의 형질전환 세포를 포함하는 형질전환 식물체로서, 칼루스 재분화를 통한 무성생식이 가능한 식물체.
- 제15항에 있어서, 단자엽식물인 형질전환 식물체.
- 제16항에 있어서, 단자엽식물인 형질전환 식물체.
- 제17항에 있어서, 벼과 식물인 형질전환 식물체.
- 제18항에 있어서, 벼과 식물인 형질전환 식물체.
- 제19항에 있어서, 벼인 형질전환 식물체.
- 제20항에 있어서, 벼인 형질전환 식물체.
- 제15항의 형질전환 식물체의 자손 또는 클론인, 칼루스 재분화를 통한 무성생식이 가능한 형질전환 식물체.
- 제16항의 형질전환 식물체의 자손 또는 클론인, 칼루스 재분화를 통한 무성생식이 가능한 형질전환 식물체.
- 제15항의 형질전환 식물체의 번식재료.
- 제16항의 형질전환 식물체의 번식재료.
- 제23항의 형질전환 식물체의 번식재료.
- 제24항의 형질전환 식물체의 번식재료.
- 제12항의 단백질에 결합하는 항체.
- 서열번호: 1 기재의 DNA와, 5x SSPE, 2x 덴하드 용액, 0.1%(w/v) SDS, 및 청어 정자 DNA 100 ㎍/ml를 포함하는 하이브리다이제이션 용액 중 25℃에서 하이브리다이즈를 행하고, 2x SSC, 0.1% SDS 중에서 25℃에서 세정하는 조건하에서 하이브리다이즈하는 핵산분자.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP36560498 | 1998-12-22 | ||
JP1998-365604 | 1998-12-22 |
Publications (2)
Publication Number | Publication Date |
---|---|
KR20010104316A KR20010104316A (ko) | 2001-11-24 |
KR100440097B1 true KR100440097B1 (ko) | 2004-07-14 |
Family
ID=18484682
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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KR10-2001-7007836A KR100440097B1 (ko) | 1998-12-22 | 1999-12-22 | 나트륨/프로톤 대향수송체, 그 수송체를 코드하는 dna, 그 수송체의 제조방법 및 그 dna를 보유하는 식물체 |
Country Status (8)
Country | Link |
---|---|
US (2) | US6861574B2 (ko) |
EP (1) | EP1143002B1 (ko) |
JP (1) | JP3955938B2 (ko) |
KR (1) | KR100440097B1 (ko) |
CN (1) | CN1309832C (ko) |
AU (1) | AU751977C (ko) |
CA (1) | CA2369590C (ko) |
WO (1) | WO2000037644A1 (ko) |
Families Citing this family (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7442852B2 (en) | 1998-03-18 | 2008-10-28 | Eduardo Blumwald | High salt plants and uses for bioremediation |
US7041875B1 (en) | 1998-03-18 | 2006-05-09 | Eduardo Blumwald | Genetic engineering salt tolerance in crop plants |
US6936750B2 (en) | 1998-03-18 | 2005-08-30 | Eduardo Blumwald | Increasing salt tolerance in plants by overexpression of a vacuolar Na+/H+ transporter[s] |
AU751977C (en) | 1998-12-22 | 2004-12-23 | National Institute Of Agrobiological Sciences | Sodium/proton antiporter gene |
ATE342984T1 (de) * | 2000-08-25 | 2006-11-15 | Basf Plant Science Gmbh | Neuartige, für na+/h+ antiporter kodierende pflanzliche polynukleotide |
US20050034191A1 (en) * | 2002-07-12 | 2005-02-10 | Eduardo Blumwald | Salt tolerant oil crops |
US20070107082A1 (en) * | 2003-06-03 | 2007-05-10 | Cropdesign N.V. | Transgenic monocotyledonous plants overexpressing a nhx protein and having improved growth characteristics and a method for making the same |
WO2005066350A1 (en) * | 2003-12-27 | 2005-07-21 | Council Of Scientific And Industrial Research | Biological process for the preparation of mineral crystals using plant seeds |
US20050214194A1 (en) * | 2004-03-23 | 2005-09-29 | Absar Ahmad | Biological process for the preparation of mineral crystals using seeds |
WO2007015268A2 (en) * | 2005-08-03 | 2007-02-08 | M.S. Swaminathan Research Foundation | Antiporter gene from porteresia coarctata for conferring stress tolerance |
CN101538573B (zh) * | 2009-04-16 | 2011-05-04 | 华中农业大学 | 利用水稻基因OsNHAD提高植物的耐盐性 |
CN105073771B (zh) * | 2013-04-22 | 2018-03-09 | 创世纪种业有限公司 | 一种小盐芥液泡膜钠氢反向转运蛋白nhx1及其编码基因与应用 |
CN115058434B (zh) * | 2022-05-20 | 2024-03-12 | 上海师范大学 | 一种调控月季花瓣颜色的基因RcNHX2及其应用 |
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US4616100A (en) * | 1984-01-26 | 1986-10-07 | Crop Development Centre Of The University Of Saskatchewan | Production of improved plants having an increased tolerance to the presence of a normally deleterious concentration of a plurality of inorganic salts |
US5272085A (en) | 1989-10-31 | 1993-12-21 | Queen's University | Sodium tolerance genes derived from schizosaccharomyces pombe |
ES2225817T3 (es) | 1991-05-09 | 2005-03-16 | Arizona Board Of Regents On Behalf Of The University Of Arizona | Plantas transgenicas con el contenido en polioles alterado. |
US5346815A (en) | 1992-08-28 | 1994-09-13 | The Mount Sinai School Of Medicine Of The City University Of New York | Sodium ion binding proteins |
US5639950A (en) | 1992-09-29 | 1997-06-17 | The Ohio State University Research Foundation | Nucleotide sequence encoding for bifunctional enzyme for proline production |
US5441875A (en) | 1993-07-23 | 1995-08-15 | Brigham And Women's Hospital | Urea transporter polypeptide |
US5780709A (en) | 1993-08-25 | 1998-07-14 | Dekalb Genetics Corporation | Transgenic maize with increased mannitol content |
US5689039A (en) | 1994-03-16 | 1997-11-18 | The University Of Tennessee Research Corporation | Plant peptide transport gene |
US5859337A (en) | 1995-06-06 | 1999-01-12 | The Regents Of The University Of California | Genes conferring salt tolerance and their uses |
CA2234168A1 (en) | 1995-10-12 | 1997-04-17 | Cornell Research Foundation, Inc. | Production of water stress or salt stress tolerant transgenic cereal plants |
US5750848A (en) | 1996-08-13 | 1998-05-12 | Monsanto Company | DNA sequence useful for the production of polyhydroxyalkanoates |
US6936750B2 (en) * | 1998-03-18 | 2005-08-30 | Eduardo Blumwald | Increasing salt tolerance in plants by overexpression of a vacuolar Na+/H+ transporter[s] |
CA2323756C (en) | 1998-03-18 | 2010-09-21 | Eduardo Blumwald | Genetic engineering salt tolerance in crop plants |
US7442852B2 (en) * | 1998-03-18 | 2008-10-28 | Eduardo Blumwald | High salt plants and uses for bioremediation |
US7041875B1 (en) * | 1998-03-18 | 2006-05-09 | Eduardo Blumwald | Genetic engineering salt tolerance in crop plants |
JP2000157287A (ja) | 1998-09-24 | 2000-06-13 | Shokubutsu Kogaku Kenkyusho:Kk | Na+/H+アンチポ―タ―蛋白質及びそれをコ―ドする遺伝子 |
AU751977C (en) | 1998-12-22 | 2004-12-23 | National Institute Of Agrobiological Sciences | Sodium/proton antiporter gene |
US20050028235A1 (en) * | 2002-07-12 | 2005-02-03 | Hong-Xia Zhang | Plant fruit with elevated potassium levels |
US20050034191A1 (en) * | 2002-07-12 | 2005-02-10 | Eduardo Blumwald | Salt tolerant oil crops |
-
1999
- 1999-12-22 AU AU17985/00A patent/AU751977C/en not_active Expired
- 1999-12-22 CN CNB998160687A patent/CN1309832C/zh not_active Expired - Lifetime
- 1999-12-22 KR KR10-2001-7007836A patent/KR100440097B1/ko active IP Right Grant
- 1999-12-22 JP JP2000589698A patent/JP3955938B2/ja not_active Expired - Lifetime
- 1999-12-22 CA CA002369590A patent/CA2369590C/en not_active Expired - Lifetime
- 1999-12-22 WO PCT/JP1999/007224 patent/WO2000037644A1/ja active IP Right Grant
- 1999-12-22 EP EP99961322.7A patent/EP1143002B1/en not_active Expired - Lifetime
-
2001
- 2001-06-22 US US09/888,035 patent/US6861574B2/en not_active Expired - Lifetime
-
2004
- 2004-09-16 US US10/944,174 patent/US7326827B2/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
US20020083487A1 (en) | 2002-06-27 |
EP1143002A1 (en) | 2001-10-10 |
WO2000037644A1 (fr) | 2000-06-29 |
AU1798500A (en) | 2000-07-12 |
US7326827B2 (en) | 2008-02-05 |
AU751977C (en) | 2004-12-23 |
CA2369590A1 (en) | 2000-06-29 |
JP3955938B2 (ja) | 2007-08-08 |
US6861574B2 (en) | 2005-03-01 |
AU751977B2 (en) | 2002-09-05 |
EP1143002A4 (en) | 2004-05-19 |
CA2369590C (en) | 2008-02-26 |
EP1143002B1 (en) | 2013-10-16 |
CN1309832C (zh) | 2007-04-11 |
CN1342203A (zh) | 2002-03-27 |
KR20010104316A (ko) | 2001-11-24 |
US20050032112A1 (en) | 2005-02-10 |
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