KR100234515B1 - The method for cultivating bean sprouts by using ozone water - Google Patents
The method for cultivating bean sprouts by using ozone water Download PDFInfo
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- KR100234515B1 KR100234515B1 KR1019970053328A KR19970053328A KR100234515B1 KR 100234515 B1 KR100234515 B1 KR 100234515B1 KR 1019970053328 A KR1019970053328 A KR 1019970053328A KR 19970053328 A KR19970053328 A KR 19970053328A KR 100234515 B1 KR100234515 B1 KR 100234515B1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
- A01G31/02—Special apparatus therefor
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
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Abstract
본 발명은 오존수로 사용하여 농약을 살포하지 않고도 청정 두과식물을 재배하는 방법에 관한 것이다.The present invention relates to a method of growing clean legumes without spraying pesticides using ozone water.
본 발명은 콩나물 재배용 원료 대두를 0.01~0.9ppm의 오존수로 0.5시간 내외의 침지처리함으로써 콩의 표면에 오염된 잔류농약의 80~100% 까지 분해, 제거시킴으로서 위생적인 콩나물을 만들 수 있으며 오염 미생물을 대두 g당 2.5×107에서 1.5×103이하로 크게 감소시켜 일체의 농약 및 비료의 사용없이 무공해 청정 콩나물을 생산할 수 있는 효과가 있다. 이밖에도 오존수로 재배하면 콩나물의 수율이 5~10% 증대될 뿐만 아니라 비린내를 없게 하고 색상과 광택을 양호하게 하며, 잔뿌리가 적은 양질의 콩나물을 얻을 수 있고, 농약등 일체의 처리없이 재배중 부패율이 현저하게 저하된다. 또한 콩나물 공장에서 발생하는 세척수가 크게 정화되어 환경산업상 유효하다.The present invention can make hygienic bean sprouts by decomposing and removing up to 80-100% of the residual pesticides contaminated on the surface of soybean sprouts by soaking the soybean sprouts raw material soybean with 0.01 ~ 0.9ppm ozone water for about 0.5 hours. It is greatly reduced from 2.5 × 10 7 to less than 1.5 × 10 3 per gram of soybean to produce clean and clean sprouts without using any pesticides and fertilizers. In addition, when grown with ozone water, the yield of bean sprouts is not only increased by 5 ~ 10%, but also makes fishy smell, improves color and luster, and obtains high quality bean sprouts with small roots. Significantly lowered. In addition, the washing water generated from the bean sprouts plant is greatly purified and effective for the environmental industry.
Description
본 발명은 오존수를 사용하여 두과식물을 재배하는 방법에 관한 것이다. 더욱 상세하게는 본 발명은 콩나물 재배용 대두를 오존수를 사용하여 농약을 살포하지 않고도 청정 두과식물을 재배하는 방법에 관한 것이다.The present invention relates to a method for cultivating legumes using ozone water. More specifically, the present invention relates to a method of growing clean legumes without spraying pesticides using soybean sprout cultivation soybeans using ozone water.
오존은 강력한 살균력을 가지는 가스상의 물질로서 농약등 유해물질을 분해하여 무독화하며 미생물을 사멸시키는 작용을 한다. 이들 농약성분의 분해와 미생물을 사멸한 후 자신은 산소로 분해되어 잔류독성을 전혀 남기지 않는 물질이다. 오존을 처리하여 만든 오존수에는 불필요한 미생물이 없을 뿐만 아니라 살균력이 강한 음이온과 산소가 풍부하게 녹아있다.Ozone is a gaseous substance with strong sterilizing power. It detoxifies harmful substances such as pesticides and kills microorganisms. After decomposing these pesticides and killing microorganisms, they are decomposed to oxygen and leave no residual toxicity. The ozone water produced by treating ozone is not only free of unnecessary microorganisms but also rich in anion and oxygen, which have strong sterilizing power.
콩나물은 단백질과 비타민류가 풍부한 식품으로 우리 민족이 전통적으로 식용하여온 주요 부식이며 비타민 C, 섬유소가 풍부한 식품이다. 특히 채소류가 부족한 겨울철에 일반 가정에서 쉽게 재배할 수 있어 국, 무침, 찌개, 탕 등으로 널리 이용되며, 특히 숙취를 해소시키는 작용이 있어 애용되며 그 소비량은 가격으로 1조원에 달한다.Sprouts are foods rich in protein and vitamins, which are the main erosion traditionally consumed by our people, and are rich in vitamin C and fiber. Especially, in winter, when vegetables are scarce, they can be easily grown at home, so it is widely used as soup, radish, stew, and soup. Especially, it has a function to relieve hangover, and its consumption reaches 1 trillion won.
그러나 산업·정보화 시대에 접어 들면서 공장규모로 생산됨에 따라 콩나물의 재배중 부패를 방지할 목적으로 성장촉진제나 농약을 사용하는 사례가 있어 국민 건강을 해칠 수 있어 안정성 면에서의 문제점들이 제기되고 있다. 콩나물 재배시에 생정조절제나 농약을 사용하는 이유로는 발아율이 낮은 콩이 오염미생물에 의하여 부패되어 콩나물의 품질이 떨어짐과 동시에 수율이 낮아지는데 그 원인이 있으며, 아울러 생장촉진제를 처리하여 콩나물의 외관을 먹음직스럽게 하고자 하는데 주된 이유가 있는 것으로 밝혀지고 있다. 콩나물의 원료로 사용되고 있는 콩의 표면에는 107내외의 미생물이 사멸되지 않은채 남아 콩나물 재배중 부패의 원인이 되고 재배 및 유통시에 처리한 잔류농약이 남을 수 있어, 이들을 제거시킴과 동시에 농약처리 없이도 먹음직스런 콩나물을 재배할 수 있는 재배법의 개발이 요구되어 왔다.However, as the industrial and informatization era enters the factory scale, there are cases of using growth accelerators or pesticides to prevent corruption during cultivation of soybean sprouts. The reason for the use of fertilizers or pesticides in the cultivation of bean sprouts is that soybeans with low germination rate are decayed by contaminating microorganisms, so that the quality of bean sprouts is lowered and the yield is lowered. It seems to have a major reason for trying to be delicious. Soybeans, which are used as raw materials for soybean sprouts, remain undisturbed in the microorganisms of about 10 7 , causing decay during soybean sprout cultivation, and residual pesticides processed during cultivation and distribution may remain. There has been a demand for the development of a cultivation method that can grow delicious bean sprouts without.
종래에도 오존수를 이용한 두과식물 재배방법이 국내특허 공개번호 제97-19834호로 공지된 바 있으나 이는 두과종자를 침지함이 없이 1.3~2.0ppm의 오존수를 직접 살포하는 방법인 것이며 더욱이 두과종자를 침지 처리하지 않기 때문에 재배기간이 길어진다는 단점이 내재되어 있었다.Conventionally, a method of cultivating legumes using ozone water has been known as Korean Patent Publication No. 97-19834, but this is a method of directly spraying 1.3-2.0 ppm of ozone water without immersing legume seeds, and further, immersion treatment of legume seeds. There is a disadvantage in that the growing period is long because it is not.
따라서, 본 발명은 상기와 같은 사실에 의거하여 안출한 것으로 예컨대 콩나물 재배용 원료 대두를 미리 오존수로 침지함으로써 콩의 표면에 오염된 잔류농약을 분해, 제거시킴으로서 위생적인 콩나물을 재배하는데 그 목적이 있다. 본 발명의 또다른 목적은 오존수로 침지하거나 살포처리하여 콩의 표면에 있는 오염 미생물을 감소시킴으로서 일체의 농약 및 비료의 사용없이 무공해 청정 콩나물을 재배하는데 그 목적이 있다.Therefore, the present invention has been made based on the above-mentioned facts, for example, the purpose of cultivating sanitary bean sprouts by decomposing and removing the residual pesticides contaminated on the surface of soybeans by immersing the soybean sprouts raw material soybean in advance with ozone water. Another object of the present invention is to cultivate pollution-free sprouts without the use of any pesticides and fertilizers by reducing the contaminating microorganisms on the surface of soybeans by dipping or spraying with ozone water.
상기와 같은 목적을 달성하기 위하여 본 발명자들은 대두를 오존처리 세척장치에 넣은 후 오존수로 침지 처리하여 오존수 세척이 콩나물의 품질과 부패 방지, 잔류농약의 분해에 미치는 영향을 조사하였고, 오존수로 세척한 대두로 콩나물을 재배하면서 오존수로 주수하였을 때의 콩나물의 품질과 부패율 및 잔류농약의 분해에 미치는 영향을 조사하였다.In order to achieve the above object, the present inventors put soybeans into an ozone treatment apparatus and immersion with ozone water to investigate the effects of ozone washing on the quality and decay of bean sprouts and decomposition of residual pesticides. The effects of soybean sprouts grown with soybean sprouts and ozonated water on the quality, decay rate and decomposition of residual pesticides were investigated.
이하, 본 발명의 구체적인 구성과 작용을 첨부한 도면과 실시예에 따라 상세히 설명한다.Hereinafter, with reference to the accompanying drawings and embodiments the specific configuration and operation of the present invention will be described in detail.
제1도는 콩의 오존수 세척시간에 따른 총균수의 변화를 나타낸 것이다.Figure 1 shows the change in total bacterial count according to the ozone water washing time of soybeans.
본 발명의 구체적인 구성과 작용을 첨부한 도면을 참고로 하여 설명한다.Detailed configurations and operations of the present invention will be described with reference to the accompanying drawings.
도 1은 콩의 오존수 세척시간에 따른 총균수 변화를 나타낸 것으로 대두 g당 2.5×107이었던 총균수가 1.5×103수준으로 크게 감소하였다. 본 발명의 오존수 처리장치나 오존수 주수장치는 실시예로서 도시한 것이며 그외의 어떠한 형태라도 본 발명의 권리범위를 제한하지 아니한다.Figure 1 shows the total bacterial count according to the ozone water washing time of soybeans, the total bacterial count was 2.5 × 10 7 / g soybeans significantly reduced to 1.5 × 10 3 level. The ozone water treatment device or ozone water pumping device of the present invention is shown as an example, and any other form does not limit the scope of the present invention.
이하, 본 발명의 구체적인 구성과 작용을 실시예를 들어 설명한다. 콩의 수침중 오존수에 의한 콩 세척 장치를 사용하여 수침중에 또는 오존을 0.01~0.9ppm으로 처리한 후 1일 9회 주수하면서 7일동안 재배하여 콩나물에 대하여 총균수, 잔류농약 및 품질특성을 비교하였다.Hereinafter, the specific structure and operation of the present invention will be described with reference to Examples. Compare soybean sprouts, total pesticides and quality characteristics of soybean sprouts during soaking with soybean sprouts using soybean washing device with ozone water during soaking or cultivation for 7 days after treating with ozone 0.01 ~ 0.9ppm It was.
[실시예 1] 총균수의 변화Example 1 Change of Total Bacteria
원료콩을 마쇄하여 멸균 0.1% peptone수로 10진법으로 희석한 후 총균수를 nutrient 배지(peptone 10g, Lab-lemico meat extract 10g, sodium chloride 5g, agar 15g, 증류수 1L, pH 7)를 사용하여 30℃에서 24~48시간 배양후 colony를 계측하였다.Grind the raw soybeans and dilute them in sterile 0.1% peptone water in 10-decimal method.The total bacterial count is 30 ℃ using nutrient medium (peptone 10g, Lab-lemico meat extract 10g, sodium chloride 5g, agar 15g, distilled water 1L, pH 7). Colony was measured after incubation for 24 to 48 hours.
실험결과, 대두에 대한 오존 처리중의 균수변화는 도 3에서 보는 바와 같이 0.4ppm으로 유지시키면 다른 처리농도에 비하여 30분 처리시의 감균효과가 현저하였으며, 0.2ppm에서도 처리시간의 경과에 따라 점차 감균효과가 높아져서 처리 3시간에는 82~83%의 감균 효과가 있었고, 0.4ppm에서는 90% 이상의 감균효과가 있었다.As a result of the experiment, the bacterial count change during ozone treatment for soybean was 0.4ppm, as shown in FIG. 3, the bactericidal effect at 30 min treatment was remarkable compared to other treatment concentrations. The sterilization effect was increased, and the treatment effect was 82-83% at 3 hours, and more than 90% at 0.4 ppm.
[실시예 2] 잔류농약의 분해Example 2 Degradation of Residual Pesticide
콩의 수침중 오존농도를 0.01~0.9ppm으로 30분 동안 처리하고 물에 7시간 30분 동안 침지한 콩에 대하여 잔류농약을 측정하였다. 실험결과는 GC(gac chromatography) 분석과 HPLC 분석으로 조사하였고, 다이아지논 등 9종의 농약을 각각 0.1ppm으로 처리하였다. 주수회수는 1일 9회(오존수 주수처리는 1일 2~3회)로 하였다. 그 결과는 하기 표 1과 같다. 표 1에서 보듯이, 물침지콩에 비하여 오존수침지콩의 잔류농약이 낮고 오존수 침지후 물재배콩나물은 더욱 낮아졌으며 오존수 침지후 오존수로 재배한 콩나물의 잔류농약은 다이아지논, 펜치온, 카벤다짐, 켑탄은 검출되지 않았다.Residual pesticides were measured for soybeans soaked in soybeans for 30 minutes at 0.01 ~ 0.9ppm and soaked in water for 7 hours and 30 minutes. The experimental results were examined by GC (gac chromatography) analysis and HPLC analysis, and each of nine pesticides, including diazinon, was treated with 0.1 ppm. The number of doubling was 9 times a day (two to three times a day for ozone doubling). The results are shown in Table 1 below. As shown in Table 1, the residual pesticides of ozone-impregnated soybeans were lower than those of soaked soybeans, and the water cultivated bean sprouts were lowered after immersion in ozone water. No peat was detected.
[실시예 3] 수침중 오존처리 콩을 물로 재배Example 3 Ozone Treated Soybeans Grown in Water
콩의 수침중 오존농도를 0.01~0.9ppm으로 하여 30분 동안 처리하고 물에 7시간 30분 동안 침지한 콩으로 콩나물을 재배하여 품질특성을 비교하였다.The soybean sprouts were soaked with soybeans soaked in water for 7 hours and 30 minutes with ozone concentration of 0.01 ~ 0.9ppm during soaking.
콩의 사용량은 250g 이었고 Top loading balance(mettler PJ3600 Delta Range, Switzerland)로 콩나물 총중량, 자엽, 배축 무게를 측정하였다. 배축길이와 뿌리길이는 Digimatic caliper(Mitutoyo, Japan)으로 측정하였다. 냄새(전혀없음 : 1점, 약간 있음 : 2점, 보통 : 3점, 많음 : 4점, 아주 많음 : 5점), 광택 및 색상은 10명의 관능요원에 의해서 자엽의 황색도와 청색도로 구별하여 측정하였다. 부패율은 콩 100개를 임으로 선택하여서 육안적으로 관찰하여 백분율(%)로 나타냈다. 그 결과는 하기 표 2와 같다.The amount of soybean used was 250g and the total weight of the bean sprouts, cotyledon and hypocotyls were measured by Top loading balance (mettler PJ3600 Delta Range, Switzerland). Axle length and root length were measured by Digimatic caliper (Mitutoyo, Japan). Odor (nothing at all: 1 point, slightly present: 2 points, moderate: 3 points, many: 4 points, very much: 5 points), gloss and color are measured by cotyledons of yellow and blue color by 10 sensory personnel It was. The decay rate was selected as 100 beans and visually observed and expressed as a percentage (%). The results are shown in Table 2 below.
실험결과, 오존으로 처리한 콩나물은 수율이 높았고, 잔뿌리가 적으며 황색도가 증가하여 색상과 광택이 양호하였다. 특히 콩이 독특한 비린 냄새가 제거되었으며 농약없이도 부패되지 않았다.As a result, the bean sprouts treated with ozone had high yields, small roots and increased yellowness, which resulted in good color and gloss. In particular, the bean has a unique fishy smell and has not been spoiled without pesticides.
[실시예 4] 물 침지한 콩을 오존수로 재배Example 4 Cultivated Soybean Soaked with Ozone Water
물에 침지한 콩(250g)을 오존수를 주수하여 콩나물을 재배하는 것외에는 상기 실시예 3과 동일하게 실험하고 품질특성을 비교하였다. 실험결과는 표 3과 같다.Soybeans (250 g) immersed in water was tested in the same manner as in Example 3 except that the bean sprouts were grown by pouring ozone water, and the quality characteristics were compared. The experimental results are shown in Table 3.
실험결과, 실시예 3과 비교할 때 수침후 오존수로 재배한 콩나물이 상기 표 3에서 보는 바와 같이 품질이 양호하였고 부패가 전혀 없었다. 같은 두과식물에 속하는 숙주나물 콩도 비슷한 결과를 나타낼 것으로 추정된다.As a result of the experiment, the soybean sprouts grown in ozone water after immersion compared to Example 3 had a good quality and no rot as shown in Table 3 above. Soybean sprouts belonging to the same legumes are expected to show similar results.
[실시예 5] 오존수에 침지한 콩을 오존수로 재배Example 5 Soybeans Soaked in Ozone Water
오존수에 침지한 콩(250g)을 오존수를 주수하여 콩나물 재배하는 것외에는 상기 실시예 3과 동일하게 실험한 후 품질특성을 비교하였다. 실험결과는 표 4와 같다.Soybeans (250 g) immersed in ozone water were tested in the same manner as in Example 3 except for cultivating bean sprouts by pouring ozone water, and then comparing the quality characteristics. The experimental results are shown in Table 4.
실험결과, 실시예 3 또는 실시예 4와 비교할 때 수침중 오존처리한 후, 오존수로 재배한 콩나물이 상기 표 4에서 보는 바와 같이 품질이 양호하였고, 부패가 전혀 없었다. 같은 두과식물에 속하는 숙주나물 콩도 비슷한 결과를 나타낼 것으로 추정된다.As a result of the experiment, the soybean sprouts grown with ozone water after the ozone treatment in water compared to Example 3 or Example 4 were of good quality as shown in Table 4 above, and there was no decay at all. Soybean sprouts belonging to the same legumes are expected to show similar results.
한편, 오존수의 농도 및 침지시간, 사용횟수에 따라 콩나물의 재배에 큰 영향을 미쳤다. 즉, 비교적 고농도의 오존수는 발아율 저하를 가져올 뿐만 아니라 생육촉진보다는 오히려 오존장해가 일어나서 생육을 저해하는 현상이 나타났다. 또한 오존수를 자주 주수하게 되면 생육장해가 더욱 가속화되어 적정농도의 오존수를 지하수와 함께 조합하여 사용하는 것이 콩나물 재배에 매우 바람직한 것으로 판단되었다.On the other hand, according to the concentration, soaking time, and the number of times of use of ozone water had a great influence on the cultivation of sprouts. In other words, relatively high concentrations of ozone water not only resulted in lower germination rates but also appeared to inhibit the growth of ozone rather than promoting growth. In addition, when ozonated water is frequently injected, growth problems are further accelerated. Therefore, it is judged to be suitable for cultivating bean sprouts by using ozone water at an appropriate concentration in combination with groundwater.
이상의 실시예와 실험결과를 통하여 설명한 바와 같이 본 발명은 오존수를 효과적인 농도와 처리방법에 의하여 사용함으로써 콩의 표면에 오염된 잔류농약의 80~100% 까지 분해, 제거시킴으로서 위생적인 콩나물을 만들 수 있으며 오염 미생물을 대두 g당 107에서 103이하로 크게 감소시킴으로서 일체의 농약 및 비료의 사용없이 무공해 청정 콩나물을 생산할 수 있는 효과가 있다. 또한 콩나물의 수율을 증대할 뿐만 아니라 비린내를 없게 하고 색상과 광택을 양호하게 하며, 잔뿌리가 적은 양질의 콩나물을 얻을 수 있으며 이밖에도 콩나물 공장에서 발생하는 세척수가 크게 정화되어 식품산업상 및 환경산업상 매우 유용한 발명인 것이다.As described through the above examples and experimental results, the present invention can make hygienic bean sprouts by decomposing and removing 80 to 100% of residual pesticides contaminated on the surface of the soybean by using ozone water by effective concentration and treatment method. By greatly reducing the contaminating microorganisms from 10 7 to less than 10 3 per g soybeans, there is an effect that can produce clean, clean sprouts without the use of any pesticides and fertilizers. In addition, it increases the yield of bean sprouts, removes fishy smell, improves color and gloss, and obtains high quality bean sprouts with few small roots. It is a useful invention.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100336612B1 (en) * | 1999-06-10 | 2002-05-16 | 대한민국(관리청:특허청장, 승계청:충남대학교총장) | Bean sprout production through irrigation water recycling using ozone to eliminate agricultural chemicals |
| KR101574707B1 (en) | 2015-05-22 | 2015-12-04 | 주식회사 애그로닉스 | Method of cultivating bean sprouts |
| KR102026692B1 (en) * | 2018-05-29 | 2019-09-30 | 이재선 | Germinating method for the growth of germinating coffee beans |
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1997
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100336612B1 (en) * | 1999-06-10 | 2002-05-16 | 대한민국(관리청:특허청장, 승계청:충남대학교총장) | Bean sprout production through irrigation water recycling using ozone to eliminate agricultural chemicals |
| KR101574707B1 (en) | 2015-05-22 | 2015-12-04 | 주식회사 애그로닉스 | Method of cultivating bean sprouts |
| KR102026692B1 (en) * | 2018-05-29 | 2019-09-30 | 이재선 | Germinating method for the growth of germinating coffee beans |
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