KR100189391B1 - Preparation of protein fodder using lysine-zymogenic biomass - Google Patents
Preparation of protein fodder using lysine-zymogenic biomass Download PDFInfo
- Publication number
- KR100189391B1 KR100189391B1 KR1019960059382A KR19960059382A KR100189391B1 KR 100189391 B1 KR100189391 B1 KR 100189391B1 KR 1019960059382 A KR1019960059382 A KR 1019960059382A KR 19960059382 A KR19960059382 A KR 19960059382A KR 100189391 B1 KR100189391 B1 KR 100189391B1
- Authority
- KR
- South Korea
- Prior art keywords
- lysine
- cells
- drying
- drum
- protein
- Prior art date
Links
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K30/00—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs
- A23K30/20—Dehydration
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Food Science & Technology (AREA)
- Fodder In General (AREA)
Abstract
본 발명은 라이신 발효균체를 이용한 단백질 사료원의 제조방법에 관한 것으로, 좀더 상세하게는 라이신 발효액에서 분리한 젖은균체를 건조하여 가축의 사료원으로 이용하는 새로운 균체 건조방법에 관한 것이다.The present invention relates to a method for producing a protein feed source using lysine fermentation cells, and more particularly to a new cell drying method for drying the wet cells isolated from the lysine fermentation broth to use as a feed source for livestock.
본 발명의 목적은 라이신 발효액에서 분리한 젖은 균체를 건조하여 가축의 단백질 사료원으로 이용하는 새로운 균체 건조 방법을 제공함에 있다.SUMMARY OF THE INVENTION An object of the present invention is to provide a new cell drying method for drying wet cells isolated from lysine fermentation broth and using them as protein feed sources for livestock.
즉, 본 발명은 라이신 발효액에서 분리한 젖은 균체를 드럼 회전속도 5.4-6.6 rpm 드럼온도 133∼137℃를 유지하면서 시간당 주입량 1.8∼2.0 kl의 운전조건으로 드림건조기에 투입하여 건조시킨 수분함량 10∼12%(w/w)의 건조균체를 얻어 단백질 사료원으로 이용함을 특징으로 한다.That is, in the present invention, the wet cells separated from the lysine fermentation broth are put into the Dream Dryer at an operating condition of 1.8 to 2.0 kl per hour while maintaining the drum rotational speed of 5.4-6.6 rpm and the drum temperature of 133 to 137 ° C. It is characterized by using as a protein feed source to obtain a dry cell of 12% (w / w).
Description
본 발명은 라이신 발효균체를 이용한 단백질 사료원의 제조방법에 관한 것으로, 좀더 상세하게는 라이신 발효액에서 분리한 젖은 균체를 건조하여 가축의 사료원으로 이용하는 새로운 균체 건조방법에 관한 것이다.The present invention relates to a method for producing a protein feed source using lysine fermentation cells, and more particularly to a new cell drying method for drying wet cells isolated from the lysine fermentation broth to use as a feed source for livestock.
라이신 발효에 사용되는 남은 균체는 단백질 함량이 70% 이상으로 가축 사료의 단백질원으로 적당하다. 또한 균체의 아미노산 조성도 가축사료의 단백질원으로 널리 쓰이는 대두박이나 어분과 유사하여 이를 단백질원으로 바람직한 일이다. 균체를 사료원으로 사용하려면 사용상의 편의를 위하여 건조후 분말상태로 제조해야 하는데 균체같은 동물성 단백질원은 건조조건에 다라 상태가 다양하게 변화되어 단백질원으로서의 가치 나아가서는 단백질원으로서의 적격유무가 결정된다.The remaining cells used for lysine fermentation have a protein content of more than 70%, making it a suitable protein source for livestock feed. In addition, the amino acid composition of the cells is similar to soybean meal or fish meal widely used as a protein source of livestock feed is a desirable thing as a protein source. In order to use the cells as a feed source, it is necessary to prepare them in powder form after drying for convenience of use. Animal protein sources such as cells are variously changed depending on drying conditions, and thus the value as a protein source, and also the eligibility as a protein source is determined. .
그 동안 발효균체를 이용한 단백질 사료원의 개발은 시도되었으나 (한국 공개 특허 제 95-16549 호, 한국공개특허 제 95-17770호) 대부분 글루타민산 제조공정을 위주로 한 것인데, 발효균체의 분리에 대해서는 상세한 논의가 있으나 분리된 젖은 균체의 건조방법에 대한 개발은 미흡했다.In the meantime, the development of protein feed sources using fermented cells has been attempted (Korea Patent Publication No. 95-16549, Korean Patent Publication No. 95-17770), but most of them focus on the manufacturing process of glutamic acid. However, there has been insufficient development of a method for drying isolated wet cells.
동물성 단백질원의 가공방법은 건조가 가장 일반적이나 라이신발효 균체의 경우 고열처리에 매우 약해 고열에 수초만 노출되어도 단백질이 변성, 파괴되어 형태나 색깔의 변화는 물론 사료가치의 저하를 관찰할 수 있다. 단백질의 변성 및 파괴는 화학분석상으로는 아미노산의 함량 저하를 나타내고 가축에서 소화율을 저하시킨다. 또한, 고열처리시 단백질이 환원당과 만나서 생기는 메일라드 반응 (Maillard Reaction)이 일어날 경우 화학분석상으로는 아미노산의 함량 변화는 구별되지 않으나 가축실험을 통한 소화율에는 커다란 변화가 나타나기 때문에 제조방법 개발의 성패는 제조물의 평가에 의해 좌우된다.Drying method of animal protein is most common, but lysine fermented cells are very weak to high heat treatment, so even if exposed to high heat for a few seconds, protein can be denatured and destroyed to change shape or color and decrease feed value. . Protein denaturation and destruction, in chemical analysis, indicates a decrease in the content of amino acids and lower digestibility in livestock. In addition, when Maillard Reaction occurs when a protein meets a reducing sugar during high heat treatment, the change of amino acid content is not distinguished by chemical analysis, but the digestibility through the livestock experiment shows a great change. Depends on its evaluation.
한편, 젖은균체는 단시간에 부패하기 때문에 저열건조는 장시간이 소요되므로 건조 도증 부패로 인한 사료가치의 저하는 물론, 독성물질의 생성 등으로 저열처리는 부적당하다.On the other hand, since the wet cells are decayed in a short time, low heat drying takes a long time, so low heat treatment is inadequate due to degradation of feed value due to dryness decay, as well as generation of toxic substances.
본 발명자들은 상기의 문제점을 해결하고자 젖은 균체의 건조방법에 대한 연구를 수행하던 중, 터널건조는 건조물 표면과 내부의 심한 온도차이로 표면은 너무 건조되고 내부가 건조되지 않아 제조물의 외관형태는 이상이 없어 보이나 소화율은 매우 저조하고, 동물성 단백원 제조에 가장 많이 쓰이는 분무건조는 다른 방법에 비해 오랜 건조시간이 요구되고, 건조후 제조물의 입자가 너무 미세하여 사료로 사용하기 위해서는 별도의 입자성형을 필요로 하기 때문에 상업화에는 문제가 있음을 알아내었다.The inventors of the present invention while studying the drying method of the wet cells to solve the above problems, tunnel drying is a surface temperature is too dry and the interior is not dried due to the severe temperature difference between the surface and the interior of the product is abnormal Although it does not seem to have a very low digestibility, spray drying, which is most used for the production of animal protein sources, requires a long drying time compared to other methods. We found that there was a problem with commercialization because it was needed.
이에 본 발명자들은 젖은 균체를 단백질 사료원으로 이용함에 있어서 수분함량에 따라 건조균체의 품질이 결정되는 것에 확인하여 연구를 수행한 결과 드럼건조에 의한 건조방법을 확립하여 본 발명을 완성하였다.Thus, the present inventors conducted a study confirming that the quality of the dry cells is determined according to the moisture content in using the wet cells as a protein feed source, and thus, the present invention was established by establishing a drying method by drum drying.
따라서, 본 발명의 목적은 라이신 발효액에서 분리한 젖은 균체를 건조하여 가축의 단백질 사료원으로 이용하는 새로운 균체 건조 방법을 제공함에 있다.Accordingly, it is an object of the present invention to provide a new cell drying method for drying wet cells isolated from lysine fermentation broth and using them as protein feed source for livestock.
즉, 본 발명은 라이신 발효액에서 분리한 젖은 균체를 드럼회전속도 5.4-6.6rpm 드럼온도 133∼137℃를 유지하면서 시간당 주입향 1.8℃2.0kl의 운전조건으로 드럼건조기에 투입하여 건조시킨 수분함량 10∼12%(w/w)의 건조균체를 단백질 사료원으로 이용함을 특징으로 한다.In other words, the present invention, the wet cells separated from the lysine fermentation broth was added to the drum dryer at an operating condition of 1.8 ℃ 2.0kl per hour injection fragrance while maintaining the drum rotation speed 5.4-6.6rpm drum temperature 133 ~ 137 ℃ 10 It is characterized by using a dry cell of ˜12% (w / w) as a protein feed source.
본 발명에 의한 건조방법을 드럼건조 방법으로, 라이신 발효액에서 분리한 젖은 균체를 드럼회전속도 5.4∼6.6rpm 바람직하게는 5.7∼6.3rpm, 드럼온도 133∼137℃ 바람직하게는 135℃를 유지하면서 시간당 주입량 1.8∼2.0kl의 운전조건으로 드럼건조기에 투입하여 건조시킨다.The drying method according to the present invention is a drum drying method, wherein the wet cells separated from the lysine fermentation broth are kept at a drum rotation speed of 5.4 to 6.6 rpm, preferably 5.7 to 6.3 rpm and a drum temperature of 133 to 137 ° C., preferably at 135 ° C. per hour. It is put into a drum dryer under the operating conditions of an injection amount of 1.8 to 2.0kl and dried.
본 발명에 건조방법에 의해 제조된 건조균체의 수분함량은 10∼12%(w/w)로 수분함량 10%(w/w)이상에서 단백질원 품질평가로 사용된 펩신소화율(Association of Official Analytical Chemists, 1990, Official Method of Analysis 971.09)이 양호했으나 곰팡이의 발생 가능성 때문에 사료원에서는 수분함량 13%(w/w)이상은 금지되어 있기 때문에, 수분함량 10∼12%(w/w)의 건조균체물이 가장 우수한 단백질 사료원으로 사용될 수 있다.The water content of the dried cells prepared by the drying method in the present invention is 10-12% (w / w), the pepsin digestion rate (Association of Official Analytical) used for quality evaluation of protein source at a water content of 10% (w / w) or more Chemists, 1990, Official Method of Analysis 971.09) was good, but water content of 10-12% (w / w) was forbidden due to the possibility of mold development. Cells can be used as the best source of protein feed.
이하 실시예에 의해 본 발명을 보다 상세히 설명한다.The present invention will be described in more detail with reference to the following examples.
(실시예 1)(Example 1)
라이신발효액에서 분리한 젖은 균체를 드럼건조기의 드럼회전속도 6.0rpm, 드럼온도 135℃로 유지하면서 시간당주입량을 1.2kl에서부터 0.2kl씩 증가시키면서 2.2kl 까지 드럼건조기에 투입하여 건조시켰다.The wet cells isolated from the lysine fermentation broth were dried by feeding the drum dryer to 2.2kl while increasing the injection rate from 1.2kl to 0.2kl in increments while maintaining the drum revolution speed of the drum dryer at a speed of 6.0 rpm and the drum temperature of 135 ° C.
건조된 균체사료의 시간당 주입량에 따른 수분함량과 펩신소화율의 변화량을 다음의 표1에 나타내었다.Changes in water content and pepsin digestion rate according to the hourly injection of dried cell feed were shown in Table 1 below.
시간당 주입량에 따른 균체사료의 수분함량과 펩신소화율의 변화Changes in Moisture Content and Pepsin Digestion Rate of Cell Cultures According to Hourly Injection
[표 1]TABLE 1
(실시예 2)(Example 2)
라이신발효액에서 분리한 젖은균체를 드럼건조기의 시간당주입량 1.8kl, 드럼온도 135℃로 유지하면서, 드럼회전속도를 5.1rpm에서부터 0.3rpm 식 증가시켜 6.6rpm까지 드럼건조기에 투입하여 건조시켰다. 건조된 균체사료의 드럼회전속도에 따른 수분함량과 펩신소화율의 변화량을 다음의 표2에 나타냈다.The wet cells separated from the lysine fermentation broth were maintained at an injection rate of 1.8kl per hour of drum dryer and a drum temperature of 135 ° C., and the drum rotation speed was increased from 5.1rpm to 0.3rpm to 6.6rpm. Changes in water content and pepsin digestion rate according to the drum rotation speed of dried cell feed were shown in Table 2 below.
드럼회전속도에 따른 균체사료의 수분함량과 펩신소화율의 변화Changes in Moisture Content and Pepsin Digestion Rate of Cell Culture According to Drum Rotation Speed
[표 2]TABLE 2
(실시예 3)(Example 3)
라이신발효액에서 분리한 젖은 균체를 드럼건조기의 시간당 주부입량 1.8kl, 드럼회전속도 6.0rpm으로 유지하면서 드럼온도를 120℃에서부터 5℃씩 증가시키면서 145℃까지 드럼건조기에 투입하여 건조시켰다. 건조된 균체사료의 드럼온도에 따른 수분함량과 펩신 소화율의 변화량을 다음의 표3에 나타내었다.The wet cells isolated from the lysine fermentation broth were put into the drum dryer at 145 ° C. while increasing the drum temperature from 120 ° C. to 5 ° C. while maintaining the drum dryer's main injection amount of 1.8 kl and the drum rotation speed of 6.0 rpm. Changes in water content and pepsin digestibility according to drum temperature of dried cell feed were shown in Table 3 below.
드럼온도에 따른 균체사료의 수분함량과 펩신소화율의 변화Changes in Water Content and Pepsin Digestion Rate of Cellulose Feeds According to Drum Temperature
[표 3]TABLE 3
(실시예 4)(Example 4)
실시예 1, 2 및 실시예3에 의해 제조된 건조균체 중에서 수분함량이 4.1, 7.3, 10.2, 11.8, 18.5%(w/w)을 선택하여 3주간 육계성장 실험을 실시하였다. 대조구는 옥수수 대두박 위주의 사료를 사용하였고 처리구에는 건조균체를 대두박과 대체하여 급여하였다. 육계 성장실험의 결과는 표4에 나타냈다.The broiler growth experiment was conducted for 3 weeks with the moisture content of 4.1, 7.3, 10.2, 11.8, 18.5% (w / w) selected from the dry cells prepared in Examples 1, 2 and 3. The control group was fed corn based soybean meal, and the treated group was fed by replacing dry cells with soybean meal. The results of broiler growth experiments are shown in Table 4.
육계 성장실험의 결과Result of broiler growth experiment
[표 4]TABLE 4
본 발명의 건조방법에 의해 제조된 건조균체물의 수분함량은 10∼12%(w/w)로 표4에서 보는 바와같이 대조구에 비해 사료의 펩신 소화율을 증대시켜 증체량을 높일수 있어 단백질 사료원으로 사용할 수 있다.The moisture content of the dried cells prepared by the drying method of the present invention is 10 to 12% (w / w) as shown in Table 4 to increase the pepsin digestibility of the feed compared to the control to increase the weight gain can be used as a protein feed source Can be.
즉, 수분함량이 10%(w/w)이하인 건조균체는 사료효율은 높일 수 있으나, 펩신 소화율이 낮아 증체량이 적고, 수분함량이 13%(w/w)이상인 건조균체는 펩신소화율은 양호하나, 곰팡이의 발생가능성 때문에 사료원으로서의 가치가 없는 것이다.In other words, dry cells with water content less than 10% (w / w) can improve feed efficiency, but low pepsin digestion yields low gain, while dry cells with water content over 13% (w / w) have good pepsin digestion rate. It is not worth it as a feed source because of the possibility of mold development.
또한, 표1, 2 및 표3에서 보는 바와 같이 펩신소화율을 열처리 정도의 기준으로 불 때 건조균체의 수분함량에 따라 펩신 소화율이 상관관계를 나타내고 있음을 알 수 있다. 즉, 열처리가 많이 될수록 수분함량이 낮고 펩신 소화율이 낮아서 사료원으로서의 가치가 저하된다.In addition, as shown in Tables 1, 2 and 3, it can be seen that the pepsin digestibility is correlated according to the moisture content of the dry cells when the pepsin digestion rate is blown based on the degree of heat treatment. That is, the more heat treatment, the lower the water content and the lower the pepsin digestibility, the lower the value as a feed source.
Claims (2)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1019960059382A KR100189391B1 (en) | 1996-11-29 | 1996-11-29 | Preparation of protein fodder using lysine-zymogenic biomass |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1019960059382A KR100189391B1 (en) | 1996-11-29 | 1996-11-29 | Preparation of protein fodder using lysine-zymogenic biomass |
Publications (2)
Publication Number | Publication Date |
---|---|
KR19980040234A KR19980040234A (en) | 1998-08-17 |
KR100189391B1 true KR100189391B1 (en) | 1999-06-01 |
Family
ID=19484487
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1019960059382A KR100189391B1 (en) | 1996-11-29 | 1996-11-29 | Preparation of protein fodder using lysine-zymogenic biomass |
Country Status (1)
Country | Link |
---|---|
KR (1) | KR100189391B1 (en) |
-
1996
- 1996-11-29 KR KR1019960059382A patent/KR100189391B1/en not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
KR19980040234A (en) | 1998-08-17 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US6146669A (en) | Method for processing oilseed material | |
SK284392A3 (en) | Feed additive for animals based on aminoacid from fermentative liquor, process for its preparation and its use | |
US6361990B1 (en) | Method for processing oilseed material | |
RU2271120C2 (en) | Lysin-containing animal feed supplement and method for production thereof | |
CN111406714A (en) | Treatment method of fresh chicken manure | |
FR2501233A1 (en) | Protein-rich product by aerobic fermentation of cellulose pastes - using the fungi Chaetomium cellulolyticum with synthetic or animal waste nutritive medium | |
Amin et al. | Proteolytic activity (aspartic endoproteinase and carboxypeptidase) of cocoa bean during fermentation | |
KR100189391B1 (en) | Preparation of protein fodder using lysine-zymogenic biomass | |
CN113101305A (en) | Fermented earthworm powder and preparation method thereof | |
KR101824132B1 (en) | A Method for Manufacturing the Un-coated Lint Containing Whole Cottonseed of Which Stability and Preference are Improved | |
RU2734079C1 (en) | Growth activator of yeast, fungi, microorganisms and crops | |
RU2189150C1 (en) | Method of producing protein-energy vitamin fodder additive on base of full-fat soya | |
RU2278166C1 (en) | Method for production of protein hydrolyzate | |
RU2124845C1 (en) | Method of preparing feed for animals | |
RU2196438C1 (en) | Method for obtaining fodder product | |
KR950009943B1 (en) | Method for preparing feed aditive | |
EP0342744B1 (en) | Procedure for the manufacturing of a base material for cattle feed and base material manufactured according to this procedure | |
SU1750604A1 (en) | Method of preparing fodder vitamin-protein concentrate | |
KR20210158170A (en) | Fermentation product of soybean Meju | |
RU2093041C1 (en) | Method of food addition preparing | |
SU1461397A1 (en) | Method of processing waste of animal origin | |
RU2073460C1 (en) | Method of preparing food addition from waste of microbiological nutrient media production | |
SU704588A1 (en) | Method of preparing feed from plants | |
RU2155496C2 (en) | Method of preparing feed from vegetable raw material | |
SU736939A1 (en) | Method of producing protein-carbohydrate fodder from wood hydrolysate |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A201 | Request for examination | ||
N231 | Notification of change of applicant | ||
E701 | Decision to grant or registration of patent right | ||
GRNT | Written decision to grant | ||
FPAY | Annual fee payment |
Payment date: 20130104 Year of fee payment: 15 |
|
FPAY | Annual fee payment |
Payment date: 20140106 Year of fee payment: 16 |
|
FPAY | Annual fee payment |
Payment date: 20150106 Year of fee payment: 17 |
|
FPAY | Annual fee payment |
Payment date: 20160107 Year of fee payment: 18 |
|
EXPY | Expiration of term |