KR0154294B1 - Novel strain candida tropicalis - Google Patents
Novel strain candida tropicalisInfo
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- KR0154294B1 KR0154294B1 KR1019950037339A KR19950037339A KR0154294B1 KR 0154294 B1 KR0154294 B1 KR 0154294B1 KR 1019950037339 A KR1019950037339 A KR 1019950037339A KR 19950037339 A KR19950037339 A KR 19950037339A KR 0154294 B1 KR0154294 B1 KR 0154294B1
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- phenol
- formaldehyde
- candida tropicalis
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/341—Consortia of bacteria
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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Abstract
본 발명은 신균주 캔디다 트로피카리스(Candida tropicalis)(KCTC 8687P)에 관한 것으로서, 더욱 상세하게는 페놀과 포름알데히드를 동시에 분해하는 능력을 갖음으로써 페놀 또는 포름알데히드를 함유하는 폐수처리에 유용하게 사용할 수 있는 신균주 캔디다 트로피카리스(Candida tropicalis)(KCTC 8687P)에 관한 것이다.The present invention relates to the new strain Candida tropicalis (KCTC 8687P), more specifically, having the ability to simultaneously decompose phenol and formaldehyde can be usefully used for the treatment of wastewater containing phenol or formaldehyde. The new strain Candida tropicalis (KCTC 8687P).
Description
[발명의 명칭][Name of invention]
신균주 캔디다 트로피카리스New Kyun Candida Tropicaris
[발명의 상세한 설명]Detailed description of the invention
본 발명은 신균주 캔디다 트로피카리스(Candida tropicalis)(KCTC 8687P)에 관한 것으로서, 더욱 상세하게는 페놀과 포름알데히드를 동시에 분해하는 능력을 갖음으로써 페놀 또는 포름알데히드를 함유하는 폐수처리에 유용하게 사용할 수 있는 신균주 캔디다 트로피카리스(Candida tropicalis)(KCTC 8687P)에 관한 것이다.The present invention relates to the new strain Candida tropicalis (KCTC 8687P), more specifically, having the ability to simultaneously decompose phenol and formaldehyde can be usefully used for the treatment of wastewater containing phenol or formaldehyde. The new strain Candida tropicalis (KCTC 8687P).
종래 정유공장, 석유화학공장, 석탄전환공장등과 같이 페놀등 난분해성 물질을 함유하고 있는 폐수처리에는 활성탄이나 다공성 유리질에 미생물을 고정화하거나, 생물막을 이용하였다.(Kotturi et al., Appl. Microbiol. Biotechnol., 34. 539, 1991 ; Ehrhardt Rehm, ibid., 30, 312, 1989 ; Morsen Rehm, ibid., 33, 206, 1990 ; Arcangeli Arvin, ibid., 37, 510, 1992 ; van Loosdrecht heijnen, Trends Biotechnol., 11, 117, 1993). 그런데, 페놀수지공장의 폐수에는 페놀외에도 포름알데히드가 고농도로 포함되어 있어 생물학적 처리가 어렵기 때문에 소각처리하고 있다. 그러나, 소각처리를 하는 경우 보다 더 유독한 2차 대기오염물질의 발생가능성이 증가한다. 또한 경제적인 면에서 보더라도 미생물을 이용하여 난분해성 물질을 처리하는 방법이 이상적이다(OECD, Biotechnology for a clean environment, 1994).Conventionally, microorganisms have been immobilized on activated carbon or porous glass or biofilms have been used for wastewater treatment containing hardly decomposable substances such as phenol, such as refineries, petrochemical plants, and coal conversion plants. (Kotturi et al., Appl.Microbiol Biotechnol., 34. 539, 1991; Ehrhardt Rehm, ibid., 30, 312, 1989; Morsen Rehm, ibid., 33, 206, 1990; Arcangeli Arvin, ibid., 37, 510, 1992; van Loosdrecht heijnen, Trends Biotechnol., 11, 117, 1993). By the way, in the waste water of the phenol resin factory, in addition to phenol, formaldehyde is contained at a high concentration, and thus biological treatment is difficult, and thus incineration is performed. However, the incidence of secondary air pollutants, which are more toxic than incineration, increases. In economic terms, it is also ideal to treat microdegradable materials using microorganisms (OECD, Biotechnology for a clean environment, 1994).
따라서, 페놀과 포름알데히드를 동시에 분해할 수 있는 미생물을 폐수처리에 이용하는 방법이 가정 적합한데, 종래 포름알데히드를 분해하는 것으로 알려진 슈도모나스 푸티다(Pseudomonas putida)는 포름알데히드의 농도가 14ppm(mg/l)이상일 경우 성장이 억제됨으로 인하여 그 적용범위가 한정적이다(서봉수 등, 국립환경연구원, v폐수의 공동처리시 효율화기법 개발에 관한 연구, 과학기술처, 1990).Therefore, it is suitable to use microorganisms capable of simultaneously decomposing phenol and formaldehyde in wastewater treatment. Pseudomonas putida, which is known to decompose formaldehyde, has a formaldehyde concentration of 14 ppm (mg / l). If the growth is inhibited, its scope of application is limited (Seo Bong-su, et al., National Institute of Environmental Research, A Study on the Development of Efficiency Techniques for Joint Treatment of Wastewater, Ministry of Science and Technology, 1990).
따라서, 본 발명자들은 상기와 같은 종래의 문제점을 극복하고자 난분해성 물질을 생물학적으로 분해시키는 연구를 진행하여온 결과 페놀과 포름알데히드를 동시에 분해하는 미생물을 폐수처리장 유래의 슬러지로부터 분리하여, 이를 캔디다 트로피카리스(Candida tropicalis)로 동정하였는 바, 종래 캔디다속(Candida sp.) 미생물은 알콜발효후 증류폐액의 처리(김영근 등, 한국산업미생물학회지, 21, 281, 1993) 등에서 사용된 예는 있으나 페놀이나 포름알데히드의 처리에 대한 보고는 전혀 없음을 확인하여 본 발명을 완성하였다.Therefore, the present inventors have conducted studies to biologically decompose hardly decomposable substances in order to overcome the conventional problems as described above, thereby separating microorganisms that simultaneously decompose phenol and formaldehyde from sludge derived from a wastewater treatment plant, and this is Candida Tropicaris. The Candida sp. Microorganism has been used in the treatment of distilled liquor after alcoholic fermentation (Kim Young-geun et al., 21, 281, 1993). The present invention was completed by confirming that there was no report on the treatment of aldehyde.
본 발명은 페놀과 포름알데히드를 동시에 분해할 수 있는 능력을 가진 캔디다 트로피카리스(Candida tropicalis)(KCTC 8687P)를 제공하는 데 그 목적이 있다.It is an object of the present invention to provide Candida tropicalis (KCTC 8687P) having the ability to simultaneously decompose phenol and formaldehyde.
이하, 본 발명을 상세히 설명하면 다음과 같다.Hereinafter, the present invention will be described in detail.
본 발명은 신균주 캔디다 트로피카리스(Candida tropicalis)(KCTC 8687P)에 관한 것이다.The present invention relates to the new strain Candida tropicalis (KCTC 8687P).
또한, 본 발명은 상기 신균주를 페놀과 포름알데히드가 함유된 폐수의 처리에 사용하는 방법에 관한 것을 포함한다.The present invention also includes a method of using the new strain for the treatment of wastewater containing phenol and formaldehyde.
이와같은 본 발명을 더욱 상세하게 설명하면 다음과 같다.The present invention will be described in more detail as follows.
본 발명은 페놀과 포름알데히드를 동시에 분해하는 능력을 가진 신균주 캔디다 트로피카리스(Candida tropicalis)(KCTC 8687P)에 관한 것으로서, 이 신균주의 분리 및 동정은 다음과 같다.The present invention relates to a new strain Candida tropicalis (KCTC 8687P) having the ability to simultaneously decompose phenol and formaldehyde, the isolation and identification of this strain is as follows.
[신균주의 분리][Isolation of mycobacteria]
먼저, 난분해성 물질을 탄소원으로 이용하도록 고안된 최소배지를 이용하여 페놀과 포름알데히드를 분해하는 미생물을 분리할 수 있다. 이때 사용되는 최소배지의 조성은 증류수 1l중에 NH4N031.0g, (NH4)2SO40.5g, MgSO40.5g, K2HPO41.0g, KH2PO40.5g, NaCl 0.5g, CaCl20.02g, FeSO40.02g이며, 미량원소 용액 1ml 첨가하였다. 이때 미량원소 용액의 조성은 증류수 1중에 CuSO450mg, H3BO3100mg, MnCl2400mg, CoCl2100mg, Na2MoO4100mg이다.(Zache Rehm, Appl. Microbiol. Biotechnol., 30, 426, 1989).First, microorganisms that decompose phenol and formaldehyde can be separated using a minimal medium designed to use a hardly decomposable substance as a carbon source. At this time, the composition of the minimum medium used was 1 g of NH 4 N0 3 1.0 g, (NH 4 ) 2 SO 4 0.5 g, MgSO 4 0.5 g, K 2 HPO 4 1.0 g, KH 2 PO 4 0.5 g, NaCl 0.5 g in 1 l of distilled water. 0.02 g of CaCl 2 and 0.02 g of FeSO 4 were added and 1 ml of a trace element solution was added. At this time, the composition of the trace element solution is 50 mg of CuSO 4 , 100 mg of H 3 BO 3, 100 mg of MnCl 2 , 100 mg of CoCl 2, and 100 mg of Na 2 MoO 4 in distilled water. (Zache Rehm, Appl.Microbiol.Biotechnol., 30, 426, 1989).
1차로 페놀을 탄소원으로 사용하는 페놀배지(페놀0.1%, 효모엑기스0.2%, 인산칼륨0.1%, 황산마그네슘0.01%, pH6.5)를 이용하여, 페놀을 탄소원으로 이용하는 미생물을 분리한다. 여기서, 분리된 미생물들을 대상으로 포름알데히드에 대하여 적용한다.First, microorganisms using phenol as a carbon source are separated using a phenol medium (phenol 0.1%, yeast extract 0.2%, potassium phosphate 0.1%, magnesium sulfate 0.01%, pH6.5) using phenol as a carbon source. Here, it is applied to formaldehyde on isolated microorganisms.
포름알데히드 배지의 조성은 인산이칼륨 0.1%, 인산칼륨 0.1%, 황산마그네슘 0.05%, 효모엑기스 0.2%를 멸균한 뒤, 포름알데히드를 250ppm이 되도록 첨가한 것이다. 이때 pH는 6.5이다.The composition of the formaldehyde medium is 0.1% dipotassium phosphate, 0.1% potassium phosphate, 0.05% magnesium sulfate, and 0.2% yeast extract, followed by addition of formaldehyde to 250 ppm. PH is 6.5.
한편, 본 발명자들은 전라북도 전주에 소재하는 제지공장의 폐수처리장 유래의 슬러지로부터 추출된 미생물들을 상기 페놀배지에서 배양하여 페놀 자화성 세균 10종과 효모 2종 그리고 곰팡이 1종을 분리하고, 이들 미생물들을 포름알데히드 배지에 다시 접종하여 페놀과 동시 이용성을 갖는 미생물을 탐색한 결과 효모 1종을 분리하였다.Meanwhile, the present inventors cultured the microorganisms extracted from the sludge derived from the wastewater treatment plant of a paper mill in Jeonju, Jeollabuk-do in the phenol medium to isolate 10 phenol magnetizable bacteria, 2 yeasts and 1 mold, After inoculating the formaldehyde medium again to search for microorganisms having co-availability with phenol, one type of yeast was isolated.
[신균주의 동정][Sympathy with mycobacteria]
상기에서 분리된 미생물의 집락은 흰색이나 크림색의 필라멘트를 형성한다. 그러나, 액체배지에서 얇은 막(pellicle)을 형성하지는 않는다. 온도 25 ~ 37℃에서는 생장하나, 42℃에서는 생장이 활발하지 못하다. 또한, 50% 농도의 포도당에서 자라며, 0.1% 사이클로 헥시미드(cycloheximide)와 페놀 0.2%에서도 생장한다.The colonies of microorganisms separated above form white or creamy filaments. However, it does not form a pellicle in the liquid medium. It grows at a temperature of 25 ~ 37 ℃, but growth is not active at 42 ℃. It also grows in 50% glucose and grows in 0.1% cycloheximide and 0.2% phenol.
본 발명의 캔디다 트로피카리스(Candida tropicalis)의 기질 이용성은 다음과 같다.Substrate availability of the Candida tropicalis (Candida tropicalis) of the present invention is as follows.
아세트산 - 포름산 -Acetic acid-formic acid-
L-글루탐산 + α-케토-글루타르산 -L-Glutamic Acid + α-Keto-Glutaric Acid-
L-말산 + 프로피온산 -L-malic acid + propionic acid-
아도니톨 + L-아라비노스 -Adonitol + L-arabinose-
D-아리비노스 - D-아라비톨 +D-Aribinose-D-Arabitol +
알부틴 - 셀로비오스 -Arbutin-Cellobiose-
덱스트린 - I-에리쓰리톨 -Dextrin-I-erythritol-
D-갈락토오즈 + 젠티비오스 -D-galactose + Gentivios-
D-글루콘산 - 2-케토-D-글루콘산 +D-gluconic acid-2-keto-D-gluconic acid +
N-아세틸-D-글루코스아민 + α-D-글루코스 +N-acetyl-D-glucoseamine + α-D-glucose +
이뉴린 - 락토오즈 -Inulin-Lactose-
말토오즈 + 말티톨 +Maltose + Maltitol +
말토트리오즈 - D-만니톨 +Maltotriose-D-Mannitol +
D-멜레지토오즈 - D-멜리비오스 -D-Melizetose-D-Melibiose-
팔라티노오즈 + L-프롤린 +Palatinoose + L-Proline +
D-라피노오즈 - L-람노오즈 -D-Rapinoose-L-Ramnoose-
D-리보오즈 - 살리신 -D-Ribose-Salisin-
D-솔비톨 + L-소르보오즈 -D-sorbitol + L-sorbose-
슈크로오즈 + D-트레할로오즈 +Sucrose + D-Trehalose +
자일리톨 - D-자일로오즈 +Xylitol-D-Xylose +
글리세롤 + 전분 -Glycerol + Starch-
에탄올 + 숙신산염 +Ethanol + Succinate +
시트르산염 +Citrate +
이상의 결과로부터 본 발명의 분리균은 캔디다 트로피카리스(Candida tropicalis)로 동정되었으며, 이를 한국과학기술연구원 부설 생명공학연구소내 유전자원센타에 1995년 9월 5일자로 기탁하여 수탁번호 KCTC 8687P를 부여받았다.From the above results, the isolated bacterium of the present invention was identified as Candida tropicalis, and it was deposited on September 5, 1995 at the Genetic Resources Center in the Biotechnology Research Institute of Korea Institute of Science and Technology and was given accession number KCTC 8687P. .
이하, 본 발명을 실시예에 의거하여 상세히 성명하면 다음과 같은 바, 본 발명이 실시예에 의해 한정되는 것은 아니다.Hereinafter, the present invention will be described in detail based on the Examples as follows, the present invention is not limited by the Examples.
[실시예 ; 신균주의 분리][Example; Isolation of mycobacteria
전라북도 전주에 소재하는 제지공장의 폐수처리장에서 슬러지 1ml를 채취하고, 물 9ml로 추출한 다음 추출액 1ml를 상기한 페놀 배지에 접종하였다. 30℃에서 배양하였을 때 페놀자화성 세균 10종과 효모 2종 및 곰팡이 1종이 분리되었다. 그리고 이들 미생물들을 상기한 포름알데히드 배지에 다시 접종하여 페놀과 동시 이용성을 갖는 미생물을 탐색하여 효모 1종을 분리하였다.1 ml of sludge was collected from a wastewater treatment plant of a paper mill in Jeonju, Jeollabuk-do, extracted with 9 ml of water, and then 1 ml of the extract was inoculated into the phenolic medium. When cultivated at 30 ° C., 10 phenolic bacteria, 2 yeasts, and 1 mold were isolated. Then, these microorganisms were inoculated again in the above-described formaldehyde medium to search for microorganisms having co-availability with phenol to isolate one species of yeast.
[실험예 1]Experimental Example 1
본 발명 실험예에서 사용된 COD(화학적 산소요구량)측정법 및 기타의 농도 측정방법은 다음과 같다.COD (chemical oxygen demand) measurement and other concentration measurement methods used in the experimental example of the present invention are as follows.
먼저 크롬을 이용한 COD 측정법은 미국 수질시험법(APHA et al., Standard methods for the examination of water and wastewater, 18th ed., APHA, Washington, 1992)에 따르며, 망간을 이용하는 방법은 환경오염 공정시험법(동화기술편집위원회, 수질오염-페기물 공정시험법, 동화기술, 1993)에 따랐다. 그리고, 페놀의 농도는 염화암모늄-암모니아 완충액(pH 10)내에서 4-아미노안티피린과 페리시안화칼륨[K3Fe(CN)6]을 첨가하여 생성된 적색의 안티피린계 색소의 흡광도를 510nm에서 측정한다(동화기술편집위원회, 수질오염-페기물 공정시험법, 동화기술, 1993). 또한, 포름알데히드의 농도는 시료 0.5ml에 1N 염산 0.5ml와 0.4% 크로모트로핀산(67% 황산에 녹인다) 4ml를 첨가하고, 15분간 항온수조에서 가열한 후 방냉하여, 580nm에서 흡광도를 측정하였다(Matsuda et al., J. Biochem., 79, 1197, 1976).First, the method of measuring COD using chromium is based on the US Water Quality Test (APHA et al., Standard methods for the examination of water and wastewater, 18th ed., APHA, Washington, 1992). (Fairy Tale Technical Editing Committee, Water Pollution-Waste Process Test, assimilation technology, 1993). The concentration of phenol was measured at 510 nm for the absorbance of the red antipyrine pigments produced by adding 4-aminoantipyrine and potassium ferricyanide [K 3 Fe (CN) 6 ] in ammonium chloride-ammonia buffer (pH 10). (Fairy tale Technical Editing Committee, Water Pollution-Waste Process Test Method, Fairytale Technology, 1993). In addition, the concentration of formaldehyde was added 0.5 ml of 1 N hydrochloric acid and 4 ml of 0.4% chromotropinic acid (dissolved in 67% sulfuric acid) in 0.5 ml of the sample, heated in a constant temperature water bath for 15 minutes, and allowed to cool. The absorbance was measured at 580 nm. (Matsuda et al., J. Biochem., 79, 1197, 1976).
먼저, 페놀 500ppm을 첨가한 YPD(효모엑기스 0.5%, 펩톤 1%. 덱스트로오즈 2%)배지에서 배양하고, 원심분리하여 회수한 캔디다 트로피카리스(Candida tropicalis)(KCTC 8687P) 균주 4 × 108cell을, 페놀을 각각 500, 1,000, 1,500, 2,000, 2,500 ppm 농도로 첨가한 인공폐수에 접종하였다. 그 결과 페놀을 0.1ppm으로 분해하는 데 소요된 시간은 페놀 농도에 따라 각각 12, 28, 39, 58 시간이었으며, 페놀농도가 2,500ppm인 경우에는 분해가 이루어지지 못하였다.First, Candida tropicalis (KCTC 8687P) strain 4 × 10 8 which was incubated in YPD (yield 0.5%, peptone 1% .dextrose 2%) medium containing 500 ppm of phenol and collected by centrifugation. The cells were inoculated into artificial wastewater containing phenol at 500, 1,000, 1,500, 2,000 and 2,500 ppm concentrations, respectively. As a result, the time taken to decompose phenol to 0.1 ppm was 12, 28, 39, and 58 hours, respectively, depending on the concentration of phenol.
[실험예 2]Experimental Example 2
상기 실험예 1의 방법으로 인공폐수에 포름알데히드를 250ppm 첨가하였다. 그리고 나서, 10, 16, 24, 30 시간 경과후 각각 포름알데히드의 농도를 측정하였으며, 그 결과는 221, 166, 84, 29ppm 이었다.250 ppm of formaldehyde was added to the artificial wastewater by the method of Experimental Example 1. Then, the concentrations of formaldehyde were measured after 10, 16, 24, and 30 hours, respectively, and the results were 221, 166, 84, and 29 ppm.
[실험예 3]Experimental Example 3
상기 실험예 1의 인공폐수에 페놀을 500ppm, 포름알데히드를 250ppm 농도로 첨가하였다. 그리고 나서, 10, 16, 24, 30시간 경과후에 페놀 농도와 포름알데히드의 농도를 측정하였으며, 그 결과 페놀 농도는 500, 500, 266, 5ppm이고, 포름알데히드의 농도는 210, 208, 20, 13ppm이었다.To the artificial wastewater of Experimental Example 1 was added 500 ppm of phenol and formaldehyde at a concentration of 250 ppm. Then, after 10, 16, 24 and 30 hours, the phenol concentration and the formaldehyde concentration were measured. As a result, the phenol concentration was 500, 500, 266, 5 ppm, and the formaldehyde concentration was 210, 208, 20, 13 ppm. It was.
[실험예 4]Experimental Example 4
상기 실험예 3에서 페놀의 농도를 1,000ppm으로 증가시켰으며, 그 결과 미생물의 생장도 억제되었고, 페놀이나 포름알데히드는 분해되지 않았다.In Experimental Example 3, the concentration of phenol was increased to 1,000 ppm. As a result, the growth of microorganisms was also inhibited, and phenol or formaldehyde was not decomposed.
[실험예 5]Experimental Example 5
부산에서 소재한 페놀 수지공장 D사의 폐수 5ml에 최소배지 200ml를 첨가하여 1:40으로 희석하고, 상기 실험예 1의 미생물 배양액을 접종하였다. 이때, 폐수 원액은 옅은 노란색으로 투명하며, pH는 3.0이었다. 폐수중 페놀의 농도는 41,000ppm이고, 포름알데히드의 농도는 2,800ppm이었다. 또한, COD(Mn)은 89,000ppm, COD(Cr)은 150,000ppm이며, BOD는 210ppm이었다. 그리고 나서, 초기, 10, 14, 18, 22, 26, 40시간 경과 후의 페놀 농도를 측정하였으며, 그 결과는 시간에 따라 각각 1,007, 921, 897, 584, 169, 142, 142ppm이었다. 이때 24시간 경과후에도 페놀 농도는 140ppm이하로 저하되지는 않았는데, 이는 폐수중 페놀 이외에 감지되는 페놀류가 함유되어 있음을 의미하며, 실제 가스크로마토그래피로 분석한 결과 소량의 나트로페놀이 검출되었다.A minimum of 200 ml was added to 5 ml of phenol resin factory D company in Busan, which was diluted 1:40 and inoculated with the microbial culture solution of Experimental Example 1. At this time, the wastewater stock solution was pale yellow and transparent, and the pH was 3.0. The concentration of phenol in the wastewater was 41,000 ppm and the concentration of formaldehyde was 2,800 ppm. Moreover, COD (Mn) was 89,000 ppm, COD (Cr) was 150,000 ppm, and BOD was 210 ppm. The phenol concentrations were then measured after initial, 10, 14, 18, 22, 26, 40 hours, and the results were 1,007, 921, 897, 584, 169, 142 and 142 ppm, respectively, over time. At this time, even after 24 hours, the phenol concentration was not lowered to 140 ppm or less, which means that phenols were detected in addition to phenol in the wastewater, and a small amount of natrophenol was detected by gas chromatography.
[실험예 6]Experimental Example 6
상기 실험예 5의 폐수 원액을 수돗물(수돗물을 받아서 이틀간 폭기하여 염소성분을 제거하였다)로 각각 20, 40, 80배로 희석하였다. 영양원은 인산이수소암모늄과 인산수소암모늄 및 염화칼슘을 1 : 1 : 0.1의 비율로 조합하여 0.2g/ㅣ씩 무기영양원으로, 효모엑기스를 0.2g/ㅣ씩 유기영양원으로 하여 희석한 폐수에 첨가하였다. 희석배수에 따른 COD(Mn),페놀,포름알데히드의 농도는 다음 표 1에 나타낸 바와 같다.The wastewater stock solution of Experimental Example 5 was diluted 20, 40 and 80 times with tap water (receiving tap water for 2 days to remove a chlorine component). Nutrients were added to the wastewater diluted with 0.2g / l of inorganic nutrient and 0.2g / l of organic nutrient by combining ammonium dihydrogen phosphate, ammonium hydrogen phosphate and calcium chloride in a ratio of 1: 1: 1. The concentrations of COD (Mn), phenol and formaldehyde according to dilution factor are shown in Table 1 below.
투명 아크릴로 제작한 10ㅣ 용량의 활성오니 반응조에 상기의 희석한 폐수를 채우고, 상기 실험예 1의 배양액과 폐수처리용 종균(Oh et al., Kor. J. Appl. Microbiol. Biotechnol., 22, 415, 1994)을 접종하고, 용존산소를 2 ~ 3ppm, 온도를 25±2℃, 체류시간을 48시간으로 유지하였다.The diluted wastewater was filled in a 10 l volume of activated sludge reactor made of transparent acrylic, and the culture solution of Experimental Example 1 and the seed for treating wastewater (Oh et al., Kor. J. Appl. Microbiol. Biotechnol., 22 , 415, 1994), dissolved oxygen 2 ~ 3ppm, temperature 25 ± 2 ℃, retention time was maintained for 48 hours.
유입수의 희석배수별로 슬러지가 안정화된 후 처리수의 수질은 다음 표 2에 나타낸 바와 같다.After sludge is stabilized for each dilution rate of the influent, the water quality of the treated water is shown in Table 2 below.
상기 표2의 결과로 부터 본 발명의 캔디다 트로피카리스(Candida tropicalis)(KCTC 8687P) 균주로 인해 COD(MN)는 97% 이상, 페놀은 99% 이상, 포름알데히드는 89%이상 제거됨을 알 수 있다.From the results of Table 2, it can be seen that more than 97% of COD (MN), more than 99% of phenol, and more than 89% of formaldehyde due to the Candida tropicalis (KCTC 8687P) strain of the present invention. .
이상의 결과로 부터 본 발명의 캔디다 트로피카리스(Candida tropicalis)(KCTC 8687P)는 페놀과 포름알데히드가 함유된 폐수 처리에 적합하여 해당산업에 유용하게 사용될 수 있다.From the above results, Candida tropicalis (KCTC 8687P) of the present invention is suitable for wastewater treatment containing phenol and formaldehyde and can be usefully used in the industry.
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| KR19980020025A (en) * | 1996-09-05 | 1998-06-25 | 김종진 | Removal of Naphthalene in Wastewater Using Microorganisms |
| KR20020012627A (en) * | 2002-01-30 | 2002-02-16 | 김두현 | A specific protein induced by aromatic surfactants |
| KR20020020601A (en) * | 2000-09-09 | 2002-03-15 | 정대원 | A specific protein induced by aromatic surfactants |
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| KR19980020025A (en) * | 1996-09-05 | 1998-06-25 | 김종진 | Removal of Naphthalene in Wastewater Using Microorganisms |
| KR20020020601A (en) * | 2000-09-09 | 2002-03-15 | 정대원 | A specific protein induced by aromatic surfactants |
| KR20020012627A (en) * | 2002-01-30 | 2002-02-16 | 김두현 | A specific protein induced by aromatic surfactants |
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