JPWO2019198238A1 - 微細藻類の培養方法 - Google Patents
微細藻類の培養方法 Download PDFInfo
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- 238000012258 culturing Methods 0.000 title claims abstract description 31
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- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241000195493 Cryptophyta Species 0.000 description 2
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- 230000005540 biological transmission Effects 0.000 description 2
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- 229940079593 drug Drugs 0.000 description 2
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- 235000013305 food Nutrition 0.000 description 2
- 239000013505 freshwater Substances 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 229920000915 polyvinyl chloride Polymers 0.000 description 2
- 239000004800 polyvinyl chloride Substances 0.000 description 2
- 230000000630 rising effect Effects 0.000 description 2
- 239000004925 Acrylic resin Substances 0.000 description 1
- 229920000178 Acrylic resin Polymers 0.000 description 1
- 241001495180 Arthrospira Species 0.000 description 1
- 241000620196 Arthrospira maxima Species 0.000 description 1
- 241001536324 Botryococcus Species 0.000 description 1
- 241001536303 Botryococcus braunii Species 0.000 description 1
- 241000195654 Chlorella sorokiniana Species 0.000 description 1
- 240000009108 Chlorella vulgaris Species 0.000 description 1
- 235000007089 Chlorella vulgaris Nutrition 0.000 description 1
- 241000195628 Chlorophyta Species 0.000 description 1
- 241001442241 Chromochloris zofingiensis Species 0.000 description 1
- 241000192700 Cyanobacteria Species 0.000 description 1
- 241000195634 Dunaliella Species 0.000 description 1
- 241000195620 Euglena Species 0.000 description 1
- 241000195619 Euglena gracilis Species 0.000 description 1
- 241000168525 Haematococcus Species 0.000 description 1
- 241001501873 Isochrysis galbana Species 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
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Abstract
Description
工程(1):微細藻類を外気から隔離されるように構成された閉鎖式の生育容器に入れ、該生育容器に収容された液体培地中で光独立栄養的に生育させる。
工程(2):前記工程(1)において前記微細藻類を生育させた前記液体培地の少なくとも一部又は全部を該微細藻類ごと前記生育容器から調整槽に移送する。
工程(3):前記工程(2)において前記調整槽に移送した前記微細藻類及び前記液体培地の混合物を該調整槽内で脱ガス処理する。
工程(4):前記工程(3)において脱ガス処理した前記微細藻類及び前記液体培地の混合物を前記調整槽から前記生育容器に移送する。
Claims (7)
- 下記の工程(1)〜工程(4)を備え、所定の生育期間には工程(1)を行い、且つ、工程(2)〜工程(4)は行なわず、工程(1)を行った後に所定の調整期間には少なくとも工程(2)〜工程(4)を行い、前記生育期間と前記調整期間とを繰り返すことを特徴とする微細藻類の培養方法。
工程(1):微細藻類を外気から隔離されるように構成された閉鎖式の生育容器に入れ、該生育容器に収容された液体培地中で光独立栄養的に生育させる。
工程(2):前記工程(1)において前記微細藻類を生育させた前記液体培地の少なくとも一部又は全部を該微細藻類ごと前記生育容器から調整槽に移送する。
工程(3):前記工程(2)において前記調整槽に移送した前記微細藻類及び前記液体培地の混合物を該調整槽内で脱ガス処理する。
工程(4):前記工程(3)において脱ガス処理した前記微細藻類及び前記液体培地の混合物を前記調整槽から前記生育容器に移送する。 - 請求項1記載の微細藻類の培養方法において、前記生育容器は水平方向に配置される横型の容器であることを特徴とする微細藻類の培養方法。
- 請求項1記載の微細藻類の培養方法において、前記微細藻類を生育させている前記液体培地の溶存酸素濃度に基づいて、前記生育期間から前記調整期間への移行時期を決定することを特徴とする微細藻類の培養方法。
- 請求項1記載の微細藻類の培養方法において、前記生育容器を水面下又は海面下に浮遊させて、前記微細藻類を光独立栄養的に生育させることを特徴とする微細藻類の培養方法。
- 請求項4記載の微細藻類の培養方法において、
前記生育容器は、一端に開口を有し、一部が伸縮性素材からなり、該開口に接続された移送管を介して前記調整槽に連通しており、
該調整槽を該調整槽の底が該開口より低い位置になるように沈降させることにより、前記微細藻類及び前記液体培地の混合物を自重により前記調整槽に移送し、
該調整槽を該調整槽の底が該開口より高い位置になるように上昇させることにより、前記微細藻類及び前記液体培地の混合物を自重により前記生育容器に移送することを特徴とする微細藻類の培養方法。 - 請求項1記載の微細藻類の培養方法において、前記生育容器に収容された前記液体培地の上方に空間を設け、該空間にキャリア気体を流通させて、前記微細藻類の生育により該空間内に溜まった酸素を排出することを特徴とする微細藻類の培養方法。
- 請求項1記載の微細藻類の培養方法において、前記生育容器を複数設けることを特徴とする微細藻類の培養方法。
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Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS59156279A (ja) * | 1983-02-28 | 1984-09-05 | Takashi Mori | 光合成反応装置 |
JPH09121835A (ja) * | 1995-10-27 | 1997-05-13 | Chikyu Kankyo Sangyo Gijutsu Kenkyu Kiko | チューブラ型フォトバイオリアクタ |
JP2005040035A (ja) * | 2003-07-25 | 2005-02-17 | Mitsubishi Heavy Ind Ltd | バイオリアクタ |
JP2013085534A (ja) * | 2011-10-20 | 2013-05-13 | Eco Renaissance Entec:Kk | 微細藻類の培養方法 |
JP2013162762A (ja) * | 2012-02-10 | 2013-08-22 | Sumitomo Heavy Ind Ltd | 光合成微生物培養装置 |
JP2015053896A (ja) * | 2013-09-12 | 2015-03-23 | 本田技研工業株式会社 | バイオマス用反応装置 |
JP2017006090A (ja) * | 2015-06-25 | 2017-01-12 | アルジー グローバル センター プロプライアタリー リミティド | 藻類回収装置、ならびに藻油を製造するためのシステム及び方法 |
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- 2018-04-13 JP JP2019560422A patent/JP6741284B2/ja active Active
- 2018-04-13 WO PCT/JP2018/015594 patent/WO2019198238A1/ja active Application Filing
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS59156279A (ja) * | 1983-02-28 | 1984-09-05 | Takashi Mori | 光合成反応装置 |
JPH09121835A (ja) * | 1995-10-27 | 1997-05-13 | Chikyu Kankyo Sangyo Gijutsu Kenkyu Kiko | チューブラ型フォトバイオリアクタ |
JP2005040035A (ja) * | 2003-07-25 | 2005-02-17 | Mitsubishi Heavy Ind Ltd | バイオリアクタ |
JP2013085534A (ja) * | 2011-10-20 | 2013-05-13 | Eco Renaissance Entec:Kk | 微細藻類の培養方法 |
JP2013162762A (ja) * | 2012-02-10 | 2013-08-22 | Sumitomo Heavy Ind Ltd | 光合成微生物培養装置 |
JP2015053896A (ja) * | 2013-09-12 | 2015-03-23 | 本田技研工業株式会社 | バイオマス用反応装置 |
JP2017006090A (ja) * | 2015-06-25 | 2017-01-12 | アルジー グローバル センター プロプライアタリー リミティド | 藻類回収装置、ならびに藻油を製造するためのシステム及び方法 |
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JP6741284B2 (ja) | 2020-08-19 |
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