JPWO2005089782A1 - Stevia fermented liquid production method, livestock health drink, and livestock breast cleaning agent - Google Patents
Stevia fermented liquid production method, livestock health drink, and livestock breast cleaning agent Download PDFInfo
- Publication number
- JPWO2005089782A1 JPWO2005089782A1 JP2006511087A JP2006511087A JPWO2005089782A1 JP WO2005089782 A1 JPWO2005089782 A1 JP WO2005089782A1 JP 2006511087 A JP2006511087 A JP 2006511087A JP 2006511087 A JP2006511087 A JP 2006511087A JP WO2005089782 A1 JPWO2005089782 A1 JP WO2005089782A1
- Authority
- JP
- Japan
- Prior art keywords
- stevia
- livestock
- fermented liquid
- cleaning agent
- breast cleaning
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
- A61P15/14—Drugs for genital or sexual disorders; Contraceptives for lactation disorders, e.g. galactorrhoea
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Organic Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Food Science & Technology (AREA)
- Animal Husbandry (AREA)
- Physiology (AREA)
- Diabetes (AREA)
- Reproductive Health (AREA)
- Hematology (AREA)
- Sustainable Development (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Pregnancy & Childbirth (AREA)
- Molecular Biology (AREA)
- Obesity (AREA)
- Gynecology & Obstetrics (AREA)
- Endocrinology (AREA)
- Oncology (AREA)
- Dermatology (AREA)
- Birds (AREA)
- Communicable Diseases (AREA)
- Alternative & Traditional Medicine (AREA)
- Botany (AREA)
Abstract
ステビアの茎部からの抽出で得られるステビア抽出液の濃縮液を熟成(発酵)させたステビア発酵液の製造方法、ステビア発酵液を含有する家畜用健康飲料、及びステビア発酵液を含有する家畜用***洗浄剤に関するものである。 ステビアの茎部に含まれているビタミンや酵母等が分解又は死滅するおそれがないステビア発酵液の製造方法、家畜用***炎治療剤等の家畜用薬剤としての効能が高い家畜用健康飲料、及び抗菌効果に優れた家畜用***洗浄剤を提供することを目的とする。 ステビアの茎部に対して活性水により45乃至60℃で抽出を行い、得られたステビア抽出液を45乃至60℃で濃縮した後、熟成させる。家畜用健康飲料や家畜用***洗浄剤は、上記の方法で製造されたステビア発酵液を含有する。Stevia fermented liquid produced by aging (fermenting) a stevia extract concentrated by extraction from stevia stems, livestock health drink containing stevia fermented liquid, and livestock containing stevia fermented liquid It relates to breast cleaning agents. Stevia fermented liquid that does not cause decomposition or death of vitamins or yeast contained in Stevia stems, livestock health drinks with high efficacy as livestock medicines such as livestock mastitis treatment agents, and An object of the present invention is to provide a livestock breast cleaning agent having an excellent antibacterial effect. Stevia stems are extracted with active water at 45 to 60 ° C., and the obtained stevia extract is concentrated at 45 to 60 ° C. and then aged. Livestock health drinks and livestock breast cleaning agents contain the stevia fermentation liquor produced by the above method.
Description
本発明は、ステビアの茎部からの抽出で得られるステビア抽出液の濃縮液を熟成(発酵)させたステビア発酵液の製造方法、ステビア発酵液を含有する家畜用健康飲料、及びステビア発酵液を含有する家畜用***洗浄剤に関するものである。 The present invention relates to a method for producing a stevia fermented liquid obtained by aging (fermenting) a concentrated stevia extract obtained by extraction from a stem portion of stevia, a health drink for livestock containing a stevia fermented liquid, and a stevia fermented liquid. The present invention relates to a breast cleaning agent for livestock.
従来のこの種の技術としては、ステビアの茎部から抽出した発酵濃縮液を有効成分とする家畜消化器系疾患治療及び体質改善薬剤(家畜用薬剤)や外用皮膚病治療剤等が知られている(例えば、特許文献1及び特許文献2参照。)。
上記の発酵濃縮液の製造に際してはステビア抽出液の濃縮液を熟成させているが、ステビアの茎部の乾燥粉末を水に入れて煮沸することによりステビア抽出液を調製しているので、ステビアの茎部に含まれているビタミンや酵母等が煮沸によって分解又は死滅することが多い。そのため、得られたステビア抽出液の濃縮液を熟成させた発酵濃縮液を有効成分とする家畜消化器系疾患治療及び体質改善薬剤や外用皮膚病治療剤としての効能(薬効)も不十分であるという問題点がある。
本発明は、以上のような事情や問題点に鑑みてなされたものであり、ステビアの茎部に含まれているビタミンや酵母等が分解又は死滅するおそれがないステビア発酵液の製造方法、家畜用***炎治療剤等の家畜用薬剤としての効能が高い家畜用健康飲料、及び抗菌効果に優れた家畜用***洗浄剤を提供することを目的とする。As this type of conventional technology, there are known livestock digestive system disease treatment and constitution improving drugs (livestock drugs), external skin disease treatment agents, etc., which contain a fermented concentrate extracted from stevia stems as an active ingredient. (For example, refer to Patent Document 1 and
In the production of the above fermentation concentrate, the stevia extract concentrate is aged, but since the stevia extract is prepared by boiling the stevia dry stem powder in water, Vitamins and yeasts contained in the stem are often decomposed or killed by boiling. Therefore, the effect (medicine effect) as a livestock digestive system disease treatment and constitution improvement drug or a topical skin disease treatment agent using the fermented concentrate obtained by aging the concentrate of the obtained stevia extract as an active ingredient is also insufficient. There is a problem.
The present invention has been made in view of the above circumstances and problems, and a method for producing a stevia fermented liquid that does not cause degradation or death of vitamins or yeasts contained in the stem portion of stevia. An object of the present invention is to provide a livestock health drink having a high efficacy as a veterinary drug such as a therapeutic agent for mastitis for livestock, and a veterinary breast cleaning agent having an excellent antibacterial effect.
本発明のステビア発酵液の製造方法は、ステビアの茎部に対して活性水により45乃至60℃で抽出を行い、得られたステビア抽出液を45乃至60℃で濃縮した後、熟成させるものである。この製造方法によれば、45乃至60℃でステビア抽出液の抽出及び濃縮を行うので、ステビアの茎部に含まれているビタミンや酵母等が分解又は死滅するおそれがない。そして、活性水を用いるので、ステビア発酵液の熟成途中や熟成後における腐敗を防止できると共に、ステビアの茎部に含まれているビタミン、ミネラル成分、糖質、脂質、タンパク質、繊維質等の抽出を効率良く行うことができる。
また、本発明のステビア発酵液の製造方法は、前記抽出を前記ステビアの茎部と前記活性水との混合物に遠赤外線を照射しながら行うものである。この製造方法によれば、抽出をより効率良く行うことができる。
更に、本発明のステビア発酵液の製造方法は、前記抽出を前記ステビアの茎部と前記活性水との混合物に振動を与えながら行うものである。この製造方法によれば、抽出をより効率良く行うことができる。
加えて、本発明の家畜用健康飲料は、上記の方法で製造されたステビア発酵液を含有するものである。この家畜用健康飲料によれば、ステビア発酵液の甘味により家畜への嗜好性に富むと共に、家畜用***炎治療剤等の家畜用薬剤としての効能が高い。
また、本発明の家畜用***洗浄剤は、上記の方法で製造されたステビア発酵液を含有するものである。この家畜用***洗浄剤によれば、抗菌効果に優れているので、家畜が***炎にかかりにくい。
更に、本発明の家畜用***洗浄剤は、前記ステビア発酵液に加えて二酸化チタン粉末を含有するものである。この家畜用***洗浄剤によれば、二酸化チタン粉末の光触媒作用により抗菌効果が更に高くなる。
また、本発明の家畜用***洗浄剤は、前記ステビア発酵液に加えて3,6−O−ジメチルキチンを含有するものである。この家畜用***洗浄剤によれば、家畜の皮膚になじみ易く(家畜の皮膚に対する親和性が高く)、ゲル状(クリーム状)の形態で家畜の***に塗布できるので、抗菌効果の持続時間を長くできると共に、乳口にすり込むように塗布することもできる。The method for producing a stevia fermented liquid according to the present invention comprises extracting stevia stems with active water at 45 to 60 ° C., concentrating the obtained stevia extract at 45 to 60 ° C., and then aging. is there. According to this production method, the stevia extract is extracted and concentrated at 45 to 60 ° C., so there is no possibility that vitamins or yeasts contained in the stem portion of stevia are decomposed or killed. And because it uses active water, it can prevent decay during and after ripening of stevia fermented liquid, and extract vitamins, mineral components, carbohydrates, lipids, proteins, fibers, etc. contained in stevia stems Can be performed efficiently.
Moreover, the manufacturing method of the stevia fermentation liquid of this invention performs the said extraction, irradiating far infrared rays to the mixture of the stem part of the said stevia, and the said active water. According to this manufacturing method, extraction can be performed more efficiently.
Furthermore, the manufacturing method of the stevia fermentation liquid of this invention performs the said extraction, giving a vibration to the mixture of the stem part of the said stevia, and the said active water. According to this manufacturing method, extraction can be performed more efficiently.
In addition, the health drink for livestock of this invention contains the stevia fermented liquid manufactured by said method. According to the health drink for livestock, the sweetness of the stevia fermented liquid has high palatability for livestock, and is highly effective as a livestock medicine such as a therapeutic agent for livestock mastitis.
Moreover, the breast cleaning agent for livestock of this invention contains the stevia fermented liquid manufactured by said method. According to the breast cleaning agent for livestock, since the antibacterial effect is excellent, the livestock is less susceptible to mastitis.
Furthermore, the livestock breast cleaning agent of the present invention contains titanium dioxide powder in addition to the stevia fermented liquid. According to this breast cleaning agent for livestock, the antibacterial effect is further enhanced by the photocatalytic action of the titanium dioxide powder.
Moreover, the breast cleaning agent for livestock of this invention contains a 3, 6-O- dimethyl chitin in addition to the said stevia fermented liquid. According to this livestock breast cleaning agent, it can be easily applied to livestock skin (high affinity for livestock skin) and can be applied to livestock breasts in the form of a gel (cream). It can be long and can also be applied to rub into the nipple.
第1図は、実施形態に係るステビア発酵液の製造方法の一例を示すフローチャートである。 FIG. 1 is a flowchart showing an example of a method for producing a stevia fermentation broth according to an embodiment.
以下、本発明の実施形態について説明する。
〔第1実施形態〕
第1実施形態に係るステビア発酵液の製造方法は、ステビアの茎部に対して活性水により45〜60℃で抽出を行い、得られたステビア抽出液を45〜60℃で濃縮した後、熟成(発酵)させるものである。この製造方法の一例を図1に示す。
ステビアとは、南米(パラグアイ)原産のキク科の植物〔学名:ステビア・レバウディアナ・ベルトーニ(Stevia rebaudiana Bertoni)〕である。このようなステビアとしては、アワユキギク(S.ovata)、ムラサキステビア(S.purprea)、イビフォリア(S.iviforia)、サリシフォリア(S.salicifolia)等が挙げられる。ステビアの茎部とは、ステビアの葉部や根部を除いた茎の部分をいう。
ステビア発酵液の製造に際しては、図1に示すように、まず、ステビアの茎部を多段式温風乾燥機等の乾燥機を用いて70℃以下、好ましくは60℃以下で数十時間乾燥(ステップS1)させた後、平均粒径15μm以下の粉末に粉砕(ステップS2)するのが望ましい。乾燥を60℃以下で行えば、ステビアの茎部に含まれているビタミンや酵母等がより分解又は死滅しにくいという利点がある。また、乾燥粉末の平均粒径を15μm以下とすれば、抽出をより効率良く行うことができる。
次いで、得られた乾燥粉末を活性水に浸漬(ステップS3)し、真空濃縮装置等の加熱装置を用いて45〜60℃で数十分間〜数時間抽出(ステップS4)を行う。活性水(改質水)とは、活性化(改質)された水をいう。水の活性化法としては、電気分解、磁化処理、電子処理、超音波処理、鉱石・ミネラル処理等のうちの1種又は2種以上の併用が挙げられる。なお、鉱石・ミネラル処理とは、水を塊状、粒状、又は粉末状の鉱石と接触させて水にミネラル成分を添加する処理をいう。鉱石としては、トルマリン(電気石)、麦飯石(ベントナイト,石英・長石班石)、医王石(石川県の医王山の戸室地区で採取される天然鉱石,別名:戸室石,学名:石英閃緑玲石)、水晶、エメラルド、ルビー、サファイヤ、アメジスト等のうちの1種又は2種以上の混合物が挙げられる。乾燥粉末と活性水との重量比は特に限定されるものではないが、
活性水/乾燥粉末=5〜7が適当である。
そして、数時間(例えば2時間)放冷(ステップS5)してからフィルタープレス等の固液分離装置(ろ過装置)を用いてろ過(ステップS6)し、ステビア抽出液をろ別する。抽出後の茎部の粉末は、上記の抽出処理に再使用してもよい。
その後、得られたステビア抽出液の濃縮(ステップS7)を、真空濃縮装置等を用いて45〜60℃で糖度が10以上(15〜25が適当)又は酸化還元電位が−30mV以下(−90〜−30mVが適当)になるまで行う。濃縮(水の留去)に要する時間は、真空度や加熱温度等により適宜に決定される。
次いで、得られた濃縮液を熟成タンク内に投入し、常温で数カ月間(例えば6カ月間)熟成(ステップS8)させる。熟成後は、フィルタープレス等の固液分離装置を用いてろ過(ステップS9)し、ステビア発酵液をろ別するのが望ましい。
このような製造方法によれば、45〜60℃でステビア抽出液の抽出及び濃縮を行うので、ステビアの茎部に含まれているビタミンや酵母等が分解又は死滅するおそれがないという利点がある。また、活性水を用いるので、ステビア発酵液の熟成途中や熟成後における腐敗を防止できると共に、ステビアの茎部に含まれているビタミン、ミネラル成分、糖質、脂質、タンパク質、繊維質等の抽出を効率良く行うことができるという利点がある。
ここで、抽出をステビアの茎部と活性水との混合物に遠赤外線を照射しながら行えば、抽出をより効率良く行うことができるという利点がある。前記混合物に遠赤外線を照射するには、前記混合物が投入される加熱装置の加熱槽の外面等に遠赤外線放射セラミック(アルミナ等)の粉末を含む塗料を塗布等しておけばよい。
抽出を前記混合物に振動を与えながら行う場合も、上記と同様の利点がある。遠赤外線の照射と振動の付与とを併用すれば、より効果的である。前記混合物に振動を与えるには、前記加熱槽等にバイブレータ又は超音波発生器等を装備しておけばよい。
〔第2実施形態〕
第2実施形態に係る家畜用健康飲料は、第1実施形態の方法で製造されたステビア発酵液を含有するものである。
家畜としては、乳牛、搾乳牛、肉牛、豚、馬、山羊、羊、鶏等が挙げられる。家畜用健康飲料は、そのままの状態で家畜に飲ませたり、あるいは家畜の飼料や飲料水その他の飲料等に添加したりすることによって、家畜に給与することができる。
このような家畜用健康飲料によれば、既述のステビア発酵液を含有しているので、ステビア発酵液の甘味により家畜への嗜好性に富むと共に、家畜用***炎治療剤等の家畜用薬剤としての効能が高いという利点がある。これは、ステビア発酵液が家畜の健康増進に有用なビタミンや酵母等を含んでいるからであると考えられる。家畜用健康飲料の効能としては、家畜用***炎治療剤としての効能の他、家畜用の気管支炎治療剤、肺炎治療剤、肝臓機能減退症(肝炎)治療剤、卵巣機能減退症(空胎)治療剤、売買による移動又は環境変化によるストレス症治療剤等としての効能が挙げられる。
〔第3実施形態〕
第3実施形態に係る家畜用***洗浄剤は、第1実施形態の方法で製造されたステビア発酵液を含有するものである。
この家畜用***洗浄剤は、そのままの状態で又は活性水等により適度な濃度に希釈してから、家畜の***に塗布することができる。塗布方法としては、家畜の***(乳頭)に非接触で塗布可能なスプレー塗布等が好適である。
このような家畜用***洗浄剤によれば、既述のステビア発酵液を含有し、***炎の原因となる黄色ブドウ球菌等の細菌やウィルス等に対する抗菌効果に優れているので、家畜が***炎にかかりにくいという利点がある。これは、ステビア発酵液が抗菌効果に優れた物質を含んでいるからであると考えられる。
ここで、家畜用***洗浄剤がステビア発酵液に加えて二酸化チタン粉末も含有していれば、二酸化チタン粉末の光触媒作用により抗菌効果が更に高くなるという利点がある。なお、二酸化チタン粉末の重量率は数wt%でよいが、その平均粒径は100nm以下であることが望ましい。二酸化チタン粉末の平均粒径が100nm以下であれば、光触媒作用に優れるので、これを含有する家畜用***洗浄剤の抗菌効果もより高くなるという利点がある。
また、家畜用***洗浄剤がステビア発酵液に加えて3,6−O−ジメチルキチン(水溶性,水溶液は中性)も含有していれば、家畜の皮膚になじみ易く(家畜の皮膚に対する親和性が高く)、ゲル状(クリーム状)の形態で家畜の***に塗布できるので、抗菌効果の持続時間を長くできると共に、乳口にすり込むように塗布することもできるという利点がある。なお、3,6−O−ジメチルキチンの重量率は数wt%でよい。また、二酸化チタン粉末と3,6−O−ジメチルキチンとを併用すれば、より効果的である。
3,6−O−ジメチルキチンは、日本国・特許第3177613号公報に記載の方法(▲1▼キチンをアルカリ処理して調製したアルカリキチン水溶液にメチル化剤を加えて撹拌、反応させることを特徴とする3,6−O−ジメチルキチンの製造方法。好ましくは、▲2▼前記メチル化剤を加える前に前記アルカリキチン水溶液を50℃以下で熟成させて脱アセチル化率が40〜60%となるように部分脱アセチル化することを特徴とする3,6−O−ジメチルキチンの製造方法。)で製造するのが好適である。上記▲1▼の方法を用いた場合は、3,6−O−ジメチルキチンの製造が容易であると共に、高分子量の3,6−O−ジメチルキチンも得られるという利点がある。また、上記▲2▼の方法を用いた場合は、アルカリキチン水溶液に不溶分が残らず、そのため、ろ過等の手間を省くことができるという利点がある。Hereinafter, embodiments of the present invention will be described.
[First Embodiment]
The manufacturing method of the stevia fermented liquid which concerns on 1st Embodiment extracts after aging at 45-60 degreeC with the active water with respect to the stalk part of stevia, and concentrates the obtained stevia extract at 45-60 degreeC, Then, it matures (Fermented). An example of this manufacturing method is shown in FIG.
Stevia is an Asteraceae plant (scientific name: Stevia rebaudiana Bertoni) native to South America (Paraguay). Examples of such stevia include Awayukikiku (S. ovata), Murasakikistevia (S. purprea), Ibifolia (S. ibifolia), Salicifolia (S. salicifolia) and the like. Stevia stem refers to the portion of the stem excluding Stevia leaves and roots.
In producing the stevia fermentation broth, as shown in FIG. 1, first, the stem portion of stevia is dried at 70 ° C. or lower, preferably 60 ° C. or lower for several tens of hours using a dryer such as a multi-stage hot air dryer ( After step S1), it is desirable to grind (step S2) into a powder having an average particle size of 15 μm or less. If drying is performed at 60 ° C. or less, there is an advantage that vitamins and yeasts contained in the stem portion of stevia are more difficult to decompose or die. If the average particle size of the dry powder is 15 μm or less, extraction can be performed more efficiently.
Next, the obtained dry powder is immersed in active water (step S3), and extraction (step S4) is performed for several tens of minutes to several hours at 45 to 60 ° C. using a heating device such as a vacuum concentrator. Active water (reformed water) refers to activated (reformed) water. Examples of the water activation method include one or more of electrolysis, magnetization treatment, electronic treatment, ultrasonic treatment, ore / mineral treatment, and the like. The ore / mineral treatment refers to a treatment in which water is brought into contact with a massive, granular, or powdered ore to add a mineral component to the water. The ores include tourmaline (electric stone), barley stone (bentonite, quartz, feldspar), Ioishi (natural ore collected in the Tomuro area of Ioyama, Ishikawa Prefecture, aka Tomuro stone, scientific name: quartz diorite. 1) or a mixture of two or more of quartz, emerald, ruby, sapphire, amethyst and the like. The weight ratio of dry powder and active water is not particularly limited,
Active water / dry powder = 5-7 is suitable.
And after standing to cool for several hours (for example, 2 hours) (step S5), it filters (step S6) using solid-liquid separators (filter apparatus), such as a filter press, and separates a stevia extract. You may reuse the powder of the stem part after extraction for said extraction process.
Thereafter, the obtained stevia extract is concentrated (step S7) by using a vacuum concentrator or the like at 45-60 ° C. with a sugar content of 10 or more (15-25 is suitable) or an oxidation-reduction potential of −30 mV or less (−90 ˜-30 mV is suitable). The time required for concentration (water distillation) is appropriately determined depending on the degree of vacuum, heating temperature, and the like.
Next, the obtained concentrated liquid is put into an aging tank and aged for several months (for example, 6 months) at room temperature (step S8). After aging, it is desirable to filter (step S9) using a solid-liquid separator such as a filter press to separate the stevia fermentation broth.
According to such a manufacturing method, the extraction and concentration of the stevia extract is performed at 45 to 60 ° C., and thus there is an advantage that vitamins and yeasts contained in the stem portion of stevia are not decomposed or killed. . In addition, since active water is used, it is possible to prevent rot during and after maturation of Stevia fermented liquid, and extraction of vitamins, mineral components, carbohydrates, lipids, proteins, fibers, etc. contained in Stevia stems There is an advantage that can be performed efficiently.
Here, if extraction is performed while irradiating a mixture of stevia stem and active water with far infrared rays, there is an advantage that extraction can be performed more efficiently. In order to irradiate the mixture with far-infrared rays, a paint containing a far-infrared radiation ceramic (alumina or the like) powder may be applied to the outer surface of a heating tank of a heating apparatus into which the mixture is charged.
There is an advantage similar to the above also when the extraction is performed while applying vibration to the mixture. It is more effective if the irradiation with far infrared rays and the application of vibration are used in combination. In order to give vibration to the mixture, the heating tank or the like may be equipped with a vibrator or an ultrasonic generator.
[Second Embodiment]
The health drink for livestock which concerns on 2nd Embodiment contains the stevia fermented liquid manufactured by the method of 1st Embodiment.
Examples of livestock include dairy cows, milked cows, beef cattle, pigs, horses, goats, sheep, and chickens. Livestock health drinks can be fed to livestock by feeding them as they are, or by adding them to livestock feed, drinking water or other beverages.
According to such a health drink for livestock, since it contains the above-mentioned stevia fermented liquid, the sweetness of the stevia fermented liquid has high palatability for livestock, and a livestock medicine such as a mastitis therapeutic agent for livestock. It has the advantage of high efficacy. This is thought to be because the stevia fermented liquid contains vitamins and yeasts that are useful for improving the health of livestock. In addition to its efficacy as a mastitis treatment agent for livestock, the health drinks for domestic animals include bronchitis treatment agents, pneumonia treatment agents, liver dysfunction (hepatitis) treatment agents, ovarian dysfunction (emptiness) ) Efficacy as a therapeutic agent, an agent for the treatment of stress due to movement by trade or environmental change.
[Third Embodiment]
The livestock breast cleaning agent according to the third embodiment contains a stevia fermented liquid produced by the method of the first embodiment.
The livestock breast cleaning agent can be applied to livestock breasts as they are or after being diluted to an appropriate concentration with active water or the like. As an application method, spray application that can be applied in a non-contact manner to the breast (nipple) of livestock is suitable.
According to such a breast cleaning agent for livestock, since it contains the aforementioned stevia fermented liquid and has an excellent antibacterial effect against bacteria and viruses such as Staphylococcus aureus that cause mastitis, There is an advantage that it is difficult to start. This is thought to be because the stevia fermented liquid contains a substance having an excellent antibacterial effect.
Here, if the breast cleaning agent for livestock contains titanium dioxide powder in addition to the stevia fermented liquid, there is an advantage that the antibacterial effect is further enhanced by the photocatalytic action of the titanium dioxide powder. The weight ratio of the titanium dioxide powder may be several wt%, but the average particle size is desirably 100 nm or less. If the average particle diameter of the titanium dioxide powder is 100 nm or less, the photocatalytic action is excellent, so that there is an advantage that the antibacterial effect of the livestock breast cleaning agent containing the titanium dioxide powder becomes higher.
In addition, if the breast cleaning agent for livestock also contains 3,6-O-dimethylchitin (water-soluble, aqueous solution is neutral) in addition to the stevia fermented liquid, it is easy to adjust to livestock skin (affinity to livestock skin) Since it can be applied to the breasts of livestock in the form of a gel (cream), the antibacterial effect can be prolonged and it can be applied so as to be rubbed into the nipple. The weight percentage of 3,6-O-dimethylchitin may be several wt%. Moreover, it is more effective if titanium dioxide powder and 3,6-O-dimethylchitin are used in combination.
3,6-O-dimethylchitin is a method described in Japanese Patent No. 3177613 (1) A methylating agent is added to an aqueous alkaline chitin solution prepared by alkali treatment of chitin and stirred and reacted. A method for producing 3,6-O-dimethylchitin, which is characterized in that, preferably, (2) the alkali chitin aqueous solution is aged at 50 ° C. or lower before the methylating agent is added, and the deacetylation rate is 40 to 60%. It is preferable to produce it by a method for producing 3,6-O-dimethylchitin, characterized by partial deacetylation so that When the method (1) is used, there is an advantage that 3,6-O-dimethylchitin can be easily produced and high molecular weight 3,6-O-dimethylchitin can be obtained. In addition, when the method (2) is used, there is an advantage that insoluble matter does not remain in the alkaline chitin aqueous solution, so that it is possible to save time and labor for filtration and the like.
次に、本発明の実施例について説明する。
〔ステビア発酵液の製造〕
ステビアとしては、アワユキギク(S.ovata)を用いた。このステビアの茎部を多段式温風乾燥機を用いて70℃で24時間乾燥させた後、平均粒径15μmの粉末に粉砕して乾燥粉末を調製した。
次いで、得られた乾燥粉末を活性水に浸漬し、真空濃縮装置を用いて60℃で2時間抽出を行った。活性水としては、交流電気分解と鉱石・ミネラル処理とを併用するミネラル還元水素水生成器〔有限会社九州電子工学製,型式:D−15,還元方式:浄水器内蔵型連続流水還元方式,鉱石:麦飯石種(石英・長石班石)、水晶鉱石種、エメラルド種、ルビー種、サファイヤ種、アメジスト種を含む28種以上の鉱石を焼石ビーズ化したガラスビーズ〕により調製したミネラル還元水素水を用いた。乾燥粉末と活性水との重量比は、活性水/乾燥粉末=5となるようにした。
そして、2時間放冷してからフィルタープレスを用いてろ過し、ステビア抽出液をろ別した。その後、得られたステビア抽出液の濃縮を、真空濃縮装置を用いて減圧下(500mmHg)かつ45℃で糖度が15になるまで行った。
次いで、得られた濃縮液を熟成タンク内に投入し、常温で6カ月間熟成させた。熟成後、フィルタープレスを用いてろ過し、ステビア発酵液をろ別した。
得られたステビア発酵液の成分分析(食品衛生法に基づく指定検査機関である財団法人広島県環境保健協会・生活科学センターが実施)の結果を表1に示す。なお、このステビア発酵液の糖度は15であり、酸化還元電位は−70mVであった。
上記で得られたステビア発酵液からなる家畜用健康飲料を、日本国・島根県内の牧場で飼育されている搾乳牛(十数頭)の飼料に1頭当たり30〜50mL/日の割合で添加した。添加を開始(開始日:2003年10月10日)してからほぼ1カ月ごとに、各搾乳牛からの搾乳に含まれる体細胞数(1mL当たり)を朝晩1回ずつ測定(検査頭数:13〜17頭)し、搾乳牛1頭当たりの平均体細胞数(1mL当たり,朝晩の平均値)を算出した。その結果を表2に示す。
実施例1と同じ牧場の搾乳牛(1頭)の飼料に家畜用健康飲料を添加しない他は、実施例1と同様の条件でその搾乳牛からの搾乳に含まれる体細胞数(1mL当たり)を朝晩1回ずつ測定し、平均体細胞数(1mL当たり,朝晩の平均値)を算出した。その結果を表2に示す。
〔実施例2(搾乳牛の慢性気管支炎に対する治験例)〕
実施例1と同様の家畜用健康飲料を、日本国・熊本県内の牧場で飼育されている搾乳牛(1頭,ホルスタイン種,雄,子牛(生後45日・体重80kg程度),慢性気管支炎を発症)が飲む人口乳に8mL/日の割合で添加した。添加を開始(開始日:2003年12月5日)してから1週間後、慢性気管支炎は完治していた。
〔実施例3(搾乳牛の肝臓機能減退症に対する治験例)〕
実施例1と同様の家畜用健康飲料を、日本国・熊本県内の牧場で飼育されている搾乳牛(1頭,ホルスタイン種,E50,2001年3月14日生,2産,慢性肝炎を発症)に100mL/日の割合で投与した。投与を開始(開始日:2003年12月8日)した後は日増しに食欲が増進し、元気回復と乳量の増加が見られ、1週間後に慢性肝炎は完治していた。
〔実施例4(肉用繁殖牛の卵巣機能減退症(静止)に対する治験例)〕
実施例1と同様の家畜用健康飲料を、日本国・熊本県内の牧場で飼育されている肉用繁殖牛〔1頭,黒毛和種,ひめふく,1996年12月26日生,2003年9月21日分娩(5産),卵巣機能減退症(静止)を発症〕に50mL/日の割合で投与した。投与を開始(開始日:2003年12月8日)してから5日後に良好な発情が来徴したので、授精した。授精してから約2カ月後、妊娠が確認された。
〔実施例5(肉用繁殖牛のストレス症に対する治験例)〕
実施例1と同様の家畜用健康飲料を、日本国・熊本県内の牧場で飼育されている肉用繁殖牛(1頭,売買による移動又は環境変化によってストレス症を発症〕に50mL/日の割合で投与した。投与を開始してから3日後、ストレス症は完治していた。
〔実施例6(搾乳牛用の***洗浄剤としての治験例)〕
既述と同様の方法で、酸化還元電位が−90mVのステビア発酵液を調製した。このステビア発酵液からなる家畜用***洗浄剤を、実施例1と同じ牧場の搾乳牛の乳頭にスプレー塗布した。塗布してから24時間経過後に乳頭の表面を脱脂綿で拭き取り、その脱脂綿を生理食塩液に浸漬して室温で30分間培養した後、生理食塩液中の一般細菌、大腸菌、及び黄色ブドウ球菌の1mL当たりの数をそれぞれ測定した。その結果を表3に示す。
実施例6と同様の家畜用***洗浄剤に二酸化チタン粉末(アナターゼ型,平均粒径10nm)を1wt%の割合で添加、混合した他は、実施例6と同様の操作を行った。その結果を表3に示す。
〔実施例8(搾乳牛の***洗浄剤としての治験例)〕
実施例6と同様の家畜用***洗浄剤に3,6−O−ジメチルキチン(脱アセチル化率:90%,重量平均分子量:12000)を2wt%の割合で添加、混合してゲル状(クリーム状)とした後、搾乳牛の乳頭に手塗りで塗布した他は、実施例6と同様の操作を行った。その結果を表3に示す。
〔実施例9(搾乳牛用の***洗浄剤としての治験例)〕
実施例8と同様の家畜用***洗浄剤を塗布してからの経過時間を120時間とした他は、実施例8と同様の操作を行った。その結果を表3に示す。
〔比較例2〕
実施例8の家畜用***洗浄剤の代わりにリッピング剤を塗布した他は、実施例8と同様の操作を行った。その結果を表3に示す。
〔比較例3〕
比較例2と同様のリッピング剤を塗布してからの経過時間を120時間とした他は、比較例2と同様の操作を行った。その結果を表3に示す。Next, examples of the present invention will be described.
[Manufacturing Stevia Fermented Liquid]
As stevia, S. ovata was used. The stevia stem was dried at 70 ° C. for 24 hours using a multistage hot air dryer, and then pulverized to a powder having an average particle size of 15 μm to prepare a dry powder.
Next, the obtained dry powder was immersed in active water and extracted at 60 ° C. for 2 hours using a vacuum concentrator. As the active water, mineral reduced hydrogen water generator that combines AC electrolysis and ore / mineral treatment (manufactured by Kyushu Electronics Co., Ltd., model: D-15, reduction method: continuous water reduction system with built-in water purifier, ore : Mineral reduced hydrogen water prepared by 28 or more kinds of ores including calcined stones (quartz and feldspar), quartz ore, emerald, ruby, sapphire, and amethyst Using. The weight ratio of the dry powder to the active water was set such that active water / dry powder = 5.
And after standing to cool for 2 hours, it filtered using the filter press and filtered the stevia extract. Thereafter, the stevia extract thus obtained was concentrated using a vacuum concentrator under reduced pressure (500 mmHg) and at 45 ° C. until the sugar content reached 15.
Next, the obtained concentrated liquid was put into an aging tank and aged at room temperature for 6 months. After aging, it was filtered using a filter press, and the stevia fermentation broth was filtered off.
Table 1 shows the results of component analysis of the stevia fermented liquid obtained (implemented by the Hiroshima Environmental Health Association / Life Science Center, which is a designated inspection organization based on the Food Sanitation Law). In addition, the sugar content of this stevia fermentation liquid was 15, and the oxidation-reduction potential was -70 mV.
The health drink for livestock consisting of the stevia fermented liquid obtained above is added at a rate of 30-50 mL / day to the feed of milking cows (ten heads) bred in a ranch in Shimane Prefecture, Japan did. Approximately every month after the start of addition (start date: October 10, 2003), the number of somatic cells contained in each milking cow (per ml) was measured once a day in the morning and night (number of test heads: 13 ˜17), and the average number of somatic cells per milking cow (average value in the morning and evening per mL) was calculated. The results are shown in Table 2.
The number of somatic cells contained in milking from the milking cow under the same conditions as in Example 1 except that the health drink for livestock is not added to the feed of the milking cow (1 head) in the same ranch as in Example 1. Was measured once in the morning and evening, and the average number of somatic cells (average value in the morning and evening per mL) was calculated. The results are shown in Table 2.
[Example 2 (Case study of chronic bronchitis in milking cow)]
The same health drink for livestock as in Example 1 was milked in a farm in Kumamoto Prefecture, Japan (1 head, Holstein, male, calf (45 days after birth, weight 80 kg)), chronic bronchitis Was added at a rate of 8 mL / day. One week after the start of the addition (start date: December 5, 2003), chronic bronchitis was completely cured.
[Example 3 (Case study for reduced liver function of milking cow)]
Milking cows (1 head, Holstein breed, E50, born on March 14, 2001, with 2 births, developing chronic hepatitis) livestock health drinks similar to those in Example 1 on a farm in Kumamoto Prefecture, Japan Was administered at a rate of 100 mL / day. After the start of administration (start date: December 8, 2003), appetite increased day by day, recovery of energy and increase in milk yield were observed, and chronic hepatitis was completely cured one week later.
[Example 4 (Clinical Trial for Hypoovarian Function (Static) in Breeding Cattle)]
A livestock health drink similar to that in Example 1 was bred on a farm in Kumamoto Prefecture, Japan [1 head, Japanese Black Breed, Himefuku, born December 26, 1996, September 2003. 21 days of delivery (5 births), hypoovarian function (stationary) developed] at a rate of 50 mL / day. Five days after the start of administration (start date: December 8, 2003), good estrus appeared and insemination was performed. About 2 months after insemination, pregnancy was confirmed.
[Example 5 (Case study of stress breeding in beef breeding cattle)]
50 mL / day of the same health drink for livestock as in Example 1 for beef breeding cattle (one animal, developing stress due to movement or environmental change) on a farm in Kumamoto Prefecture, Japan Three days after the start of administration, the stress disorder was completely cured.
[Example 6 (Clinical trial as a breast cleaning agent for milking cows)]
A stevia fermentation broth having a redox potential of −90 mV was prepared in the same manner as described above. The livestock breast cleaning agent comprising this stevia fermented liquid was sprayed onto the nipples of milking cows in the same ranch as in Example 1. 24 hours after application, the surface of the nipple was wiped with absorbent cotton, the absorbent cotton was immersed in physiological saline and incubated at room temperature for 30 minutes, and then 1 mL of general bacteria, Escherichia coli, and Staphylococcus aureus in the physiological saline. Each hit was measured. The results are shown in Table 3.
The same operation as in Example 6 was performed, except that titanium dioxide powder (anatase type, average particle size 10 nm) was added and mixed in the same amount as in Example 6 for a livestock breast cleaning agent. The results are shown in Table 3.
[Example 8 (Clinical Trial as Breast Cleaning Agent for Milking Cows)]
3,6-O-dimethylchitin (deacetylation rate: 90%, weight average molecular weight: 12000) was added to the same livestock breast cleaning agent as in Example 6 at a rate of 2 wt%, and mixed to form a gel (cream) Then, the same operation as in Example 6 was performed, except that it was applied by hand to the nipple of a milking cow. The results are shown in Table 3.
[Example 9 (Clinical trial as a breast cleaning agent for milking cows)]
The same operation as in Example 8 was performed, except that the elapsed time after applying the same livestock breast cleaning agent as in Example 8 was 120 hours. The results are shown in Table 3.
[Comparative Example 2]
The same operation as in Example 8 was performed except that a ripping agent was applied instead of the livestock breast cleaning agent in Example 8. The results are shown in Table 3.
[Comparative Example 3]
The same operation as in Comparative Example 2 was performed, except that the elapsed time after applying the same ripping agent as in Comparative Example 2 was 120 hours. The results are shown in Table 3.
以上のように、本発明に係るステビア発酵液の製造方法は、ステビアの茎部に含まれているビタミンや酵母等が分解又は死滅しないようにする方法として有用である。また、本発明に係る家畜用健康飲料は、家畜用***炎治療剤等の家畜用薬剤としての効能が高い家畜用の健康飲料として有用である。更に、本発明に係る家畜用***洗浄剤は、抗菌効果に優れた家畜用の***洗浄剤として有用である。 As mentioned above, the manufacturing method of the stevia fermented liquid which concerns on this invention is useful as a method which prevents the vitamin, yeast, etc. which are contained in the stem part of stevia from decomposing | disassembling or dying. The health drink for livestock according to the present invention is useful as a health drink for livestock having high efficacy as a livestock medicine such as a therapeutic agent for livestock mastitis. Furthermore, the livestock breast cleaning agent according to the present invention is useful as a livestock breast cleaning agent having an excellent antibacterial effect.
Claims (7)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PCT/JP2004/003596 WO2005089782A1 (en) | 2004-03-17 | 2004-03-17 | Process for producing fermented stevia solution, health drink for livestock and udder wasiing liquor for livestock |
Publications (1)
Publication Number | Publication Date |
---|---|
JPWO2005089782A1 true JPWO2005089782A1 (en) | 2008-01-31 |
Family
ID=34993440
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2006511087A Pending JPWO2005089782A1 (en) | 2004-03-17 | 2004-03-17 | Stevia fermented liquid production method, livestock health drink, and livestock breast cleaning agent |
Country Status (2)
Country | Link |
---|---|
JP (1) | JPWO2005089782A1 (en) |
WO (1) | WO2005089782A1 (en) |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
NZ585720A (en) * | 2007-12-03 | 2012-05-25 | Dsm Ip Assets Bv | Novel nutraceutical compositions containing stevia extract or stevia extract constituents and uses thereof |
DE102008009800A1 (en) * | 2008-02-18 | 2009-08-20 | Andresen, Uwe, Prof. Dr. | Method for the preventive treatment of health problems in calving cows |
US11730683B2 (en) | 2011-10-28 | 2023-08-22 | TaylorBaby, LLC | Flavored wipe and dispensing system |
WO2013063102A1 (en) | 2011-10-28 | 2013-05-02 | TaylorBaby, LLC | Flavored wipe and dispensing system |
CN105166451A (en) * | 2015-08-06 | 2015-12-23 | 钱峰 | Cow feed capable of preventing and treating mammitis |
GR20170100314A (en) * | 2017-07-04 | 2019-04-04 | Ηλιας Θεοδωρου Τσαμπαρδουκας | A cosmetic and therapeutic product produced from stevia's stalk and leaf extract |
CN110638852B (en) * | 2019-09-16 | 2022-05-17 | 上海维丘康敬生物科技有限公司 | Pure natural plant antioxidant derived from natural stevia rebaudiana and preparation method thereof |
Citations (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH02211202A (en) * | 1989-02-10 | 1990-08-22 | Nippon Shakai Iryo Kenkyusho:Kk | Ultrasonic extractor and extraction of crude drug using the same |
JPH05124973A (en) * | 1991-11-06 | 1993-05-21 | Masayuki Otsuki | Extraction process of chinese galenical drug and apparatus therefor |
JPH05320064A (en) * | 1992-05-18 | 1993-12-03 | Takashi Sato | Method for decocting material to be decocted |
JPH0713022B2 (en) * | 1991-09-10 | 1995-02-15 | 文夫 堂園 | Veterinary medicine |
JPH08649A (en) * | 1994-06-16 | 1996-01-09 | Nippon Intec Kk | Tait cup and inflammation-suppressive liquid also used for cleaning nipple |
JPH08112046A (en) * | 1994-10-17 | 1996-05-07 | Nippon Intec Kk | Teat cup also useful for cleaning teat |
JPH10120706A (en) * | 1996-10-17 | 1998-05-12 | Nippon Kireeto Kk | Production of 3,6-o-dimethylchitin and cosmetic material and growth promoter for plant comprising the same |
JPH10330279A (en) * | 1997-03-31 | 1998-12-15 | Naohiko Sato | Substance having antihistamic activity |
JPH1143443A (en) * | 1997-05-27 | 1999-02-16 | Naohiko Sato | Selective bactericide against food-poisoning bacteria |
JP2001275587A (en) * | 2000-03-31 | 2001-10-09 | Shiyaburon:Kk | Method of extracting propolis and food including the same |
JP2002003326A (en) * | 2000-06-23 | 2002-01-09 | Ito En Ltd | Antimicrobial agent containing tea as component |
JP2004067591A (en) * | 2002-08-06 | 2004-03-04 | Hokuto:Kk | Curcuma domestica extract liquid composition |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0713021B2 (en) * | 1991-08-01 | 1995-02-15 | 文夫 堂園 | Oral drug for treatment of digestive system diseases |
US5262161A (en) * | 1991-09-30 | 1993-11-16 | Fumio Dozono | Stevia extract-containing medicine |
-
2004
- 2004-03-17 WO PCT/JP2004/003596 patent/WO2005089782A1/en active Application Filing
- 2004-03-17 JP JP2006511087A patent/JPWO2005089782A1/en active Pending
Patent Citations (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH02211202A (en) * | 1989-02-10 | 1990-08-22 | Nippon Shakai Iryo Kenkyusho:Kk | Ultrasonic extractor and extraction of crude drug using the same |
JPH0713022B2 (en) * | 1991-09-10 | 1995-02-15 | 文夫 堂園 | Veterinary medicine |
JPH05124973A (en) * | 1991-11-06 | 1993-05-21 | Masayuki Otsuki | Extraction process of chinese galenical drug and apparatus therefor |
JPH05320064A (en) * | 1992-05-18 | 1993-12-03 | Takashi Sato | Method for decocting material to be decocted |
JPH08649A (en) * | 1994-06-16 | 1996-01-09 | Nippon Intec Kk | Tait cup and inflammation-suppressive liquid also used for cleaning nipple |
JPH08112046A (en) * | 1994-10-17 | 1996-05-07 | Nippon Intec Kk | Teat cup also useful for cleaning teat |
JPH10120706A (en) * | 1996-10-17 | 1998-05-12 | Nippon Kireeto Kk | Production of 3,6-o-dimethylchitin and cosmetic material and growth promoter for plant comprising the same |
JPH10330279A (en) * | 1997-03-31 | 1998-12-15 | Naohiko Sato | Substance having antihistamic activity |
JPH1143443A (en) * | 1997-05-27 | 1999-02-16 | Naohiko Sato | Selective bactericide against food-poisoning bacteria |
JP2001275587A (en) * | 2000-03-31 | 2001-10-09 | Shiyaburon:Kk | Method of extracting propolis and food including the same |
JP2002003326A (en) * | 2000-06-23 | 2002-01-09 | Ito En Ltd | Antimicrobial agent containing tea as component |
JP2004067591A (en) * | 2002-08-06 | 2004-03-04 | Hokuto:Kk | Curcuma domestica extract liquid composition |
Also Published As
Publication number | Publication date |
---|---|
WO2005089782A1 (en) | 2005-09-29 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JP6462887B2 (en) | A drinking agent and a feed composition having a function of removing malodor from pet urine, comprising a mixture of natural extracts as an active ingredient | |
CN104664161A (en) | Lean-type selenium-enriched pig feed for reducing cholesterol | |
EP0481396A2 (en) | Tannin extract of sweet chestnut wood, a process for the preparation thereof and its use | |
RU2568582C1 (en) | Method for non-specific immunoprophylaxis of bovine respiratory diseases | |
JPWO2005089782A1 (en) | Stevia fermented liquid production method, livestock health drink, and livestock breast cleaning agent | |
RU2473354C1 (en) | Method for increasing non-specific resistance and productivity of young bovine animals | |
US5250300A (en) | Method for treating digestive organ disorders of domestic animals | |
JP2009209057A (en) | Immunoactivator and its manufacturing method, and foodstuff for human being, fodder for animal or domestic animal, immunoactivator for plant, and cosmetic raw material, each comprising the immunoactivator | |
CN104225090B (en) | A kind of preparation for increasing milk production of cow and preparation method thereof | |
CN110692834A (en) | Feed for preventing constipation of sows and promoting lactation of sows and preparation method thereof | |
RU2319391C2 (en) | Feed additive for pigs and method of pig feeding | |
KR20110044516A (en) | A method manufacturing feed and water additives by using groats or a polar solvent extract of illicium verum, agastache rugosa and atractylodes japonica | |
CN101370391A (en) | Morinda citrifolia enhanced products for administration to animal | |
CN108850541A (en) | A kind of feed addictive and preparation method thereof of the alternative antibiotic of disease preventing and treating | |
CN103385368B (en) | Chinese medicine preparation for preventing and treating postpartum hypogalactia of sow and production method thereof | |
CN101332275A (en) | Baby pig fattening decoction | |
RU2370274C1 (en) | Bioactive veterinary drug and method of non-specific resistanse improval for calves using drug | |
CN109845697A (en) | A kind of cultural method of fructus lycii sheep | |
RU2371191C1 (en) | Bioaactive stimulation agent and correction method applying agent for secondary immunodeficiency of calves | |
JP2005124536A (en) | Feed additive for livestock and method for producing the same | |
WO2008002005A1 (en) | A method for preparing pig placental extract to use as a feed additive and use thereof | |
JP3788689B2 (en) | Livestock health promotion methods | |
RU2114625C1 (en) | Method of biostimulating agent preparing | |
CN106387431A (en) | Feed additive with effect of improving sow farrowing capacity | |
CN106071211A (en) | A kind of nursery piglet compound feed containing high-activity lactic acid bacteria and preparation method thereof |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20070319 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20100812 |
|
A02 | Decision of refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A02 Effective date: 20101202 |