JPS6480284A - 5'-nucleotidase and production thereof - Google Patents
5'-nucleotidase and production thereofInfo
- Publication number
- JPS6480284A JPS6480284A JP62236144A JP23614487A JPS6480284A JP S6480284 A JPS6480284 A JP S6480284A JP 62236144 A JP62236144 A JP 62236144A JP 23614487 A JP23614487 A JP 23614487A JP S6480284 A JPS6480284 A JP S6480284A
- Authority
- JP
- Japan
- Prior art keywords
- ferm
- microbacterium
- arthrobacter
- culture medium
- corynebacterium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Enzymes And Modification Thereof (AREA)
Abstract
PURPOSE:To obtain the titled enzyme useful for determining 5'-nucleotide in urine, blood serum, etc., or inosine 5'-monophosphate (IMP) content, etc., in a food and drink, etc., by cultivating a microorganism belonging to the genius Corynebacterium, Arthrobacter or Microbacterium in a culture medium. CONSTITUTION:A microorganism, such as Corynebacterium murisepticum No.7 (FERM-P No.206), Arthrobacter 11 (FERM-P No.207), Microbacterium No.205 (FERM-P No.4923) or Microbacterium No.205-MpCaGu-1 (FERM-P No.7102), in aerobically cultivated in a culture medium containing a carbon source, nitrogen source, inorganic phosphate, vitamins, etc., and 0.001-500mg/l cyclic-3',5'- nucleotide inhibitor added thereto to provide a cultivation filtrate. A 5'- nucleotidase, having the optimum pH 7.9-8.3 and capable of decomposing 5'-deoxyribonulceotide or 5'-ribonucleotide to form nucleosides and phosphoric acid is obtained from the above-mentioned resultant cultivation filtrate.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP62236144A JPS6480284A (en) | 1987-09-22 | 1987-09-22 | 5'-nucleotidase and production thereof |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP62236144A JPS6480284A (en) | 1987-09-22 | 1987-09-22 | 5'-nucleotidase and production thereof |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS6480284A true JPS6480284A (en) | 1989-03-27 |
Family
ID=16996410
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP62236144A Pending JPS6480284A (en) | 1987-09-22 | 1987-09-22 | 5'-nucleotidase and production thereof |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS6480284A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN115433724A (en) * | 2022-05-30 | 2022-12-06 | 河北艾欧路生物科技有限责任公司 | Method for extracting 5-nucleotidase from pork liver |
-
1987
- 1987-09-22 JP JP62236144A patent/JPS6480284A/en active Pending
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN115433724A (en) * | 2022-05-30 | 2022-12-06 | 河北艾欧路生物科技有限责任公司 | Method for extracting 5-nucleotidase from pork liver |
CN115433724B (en) * | 2022-05-30 | 2023-04-28 | 河北艾欧路生物科技有限责任公司 | Method for extracting 5-nucleotidase from pig liver |
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