JPS6480284A - 5'-nucleotidase and production thereof - Google Patents

5'-nucleotidase and production thereof

Info

Publication number
JPS6480284A
JPS6480284A JP62236144A JP23614487A JPS6480284A JP S6480284 A JPS6480284 A JP S6480284A JP 62236144 A JP62236144 A JP 62236144A JP 23614487 A JP23614487 A JP 23614487A JP S6480284 A JPS6480284 A JP S6480284A
Authority
JP
Japan
Prior art keywords
ferm
microbacterium
arthrobacter
culture medium
corynebacterium
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP62236144A
Other languages
Japanese (ja)
Inventor
Jiro Ishiyama
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kikkoman Corp
Original Assignee
Kikkoman Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kikkoman Corp filed Critical Kikkoman Corp
Priority to JP62236144A priority Critical patent/JPS6480284A/en
Publication of JPS6480284A publication Critical patent/JPS6480284A/en
Pending legal-status Critical Current

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  • Enzymes And Modification Thereof (AREA)

Abstract

PURPOSE:To obtain the titled enzyme useful for determining 5'-nucleotide in urine, blood serum, etc., or inosine 5'-monophosphate (IMP) content, etc., in a food and drink, etc., by cultivating a microorganism belonging to the genius Corynebacterium, Arthrobacter or Microbacterium in a culture medium. CONSTITUTION:A microorganism, such as Corynebacterium murisepticum No.7 (FERM-P No.206), Arthrobacter 11 (FERM-P No.207), Microbacterium No.205 (FERM-P No.4923) or Microbacterium No.205-MpCaGu-1 (FERM-P No.7102), in aerobically cultivated in a culture medium containing a carbon source, nitrogen source, inorganic phosphate, vitamins, etc., and 0.001-500mg/l cyclic-3',5'- nucleotide inhibitor added thereto to provide a cultivation filtrate. A 5'- nucleotidase, having the optimum pH 7.9-8.3 and capable of decomposing 5'-deoxyribonulceotide or 5'-ribonucleotide to form nucleosides and phosphoric acid is obtained from the above-mentioned resultant cultivation filtrate.
JP62236144A 1987-09-22 1987-09-22 5'-nucleotidase and production thereof Pending JPS6480284A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP62236144A JPS6480284A (en) 1987-09-22 1987-09-22 5'-nucleotidase and production thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP62236144A JPS6480284A (en) 1987-09-22 1987-09-22 5'-nucleotidase and production thereof

Publications (1)

Publication Number Publication Date
JPS6480284A true JPS6480284A (en) 1989-03-27

Family

ID=16996410

Family Applications (1)

Application Number Title Priority Date Filing Date
JP62236144A Pending JPS6480284A (en) 1987-09-22 1987-09-22 5'-nucleotidase and production thereof

Country Status (1)

Country Link
JP (1) JPS6480284A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115433724A (en) * 2022-05-30 2022-12-06 河北艾欧路生物科技有限责任公司 Method for extracting 5-nucleotidase from pork liver

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115433724A (en) * 2022-05-30 2022-12-06 河北艾欧路生物科技有限责任公司 Method for extracting 5-nucleotidase from pork liver
CN115433724B (en) * 2022-05-30 2023-04-28 河北艾欧路生物科技有限责任公司 Method for extracting 5-nucleotidase from pig liver

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