JPS6474451A - Sell selection device - Google Patents

Sell selection device

Info

Publication number
JPS6474451A
JPS6474451A JP23170887A JP23170887A JPS6474451A JP S6474451 A JPS6474451 A JP S6474451A JP 23170887 A JP23170887 A JP 23170887A JP 23170887 A JP23170887 A JP 23170887A JP S6474451 A JPS6474451 A JP S6474451A
Authority
JP
Japan
Prior art keywords
cells
cell
laser light
light
control section
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP23170887A
Other languages
Japanese (ja)
Inventor
Seiki Ashihara
Ryuichi Tate
Akiko Shinoda
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Resonac Holdings Corp
Original Assignee
Showa Denko KK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Showa Denko KK filed Critical Showa Denko KK
Priority to JP23170887A priority Critical patent/JPS6474451A/en
Publication of JPS6474451A publication Critical patent/JPS6474451A/en
Pending legal-status Critical Current

Links

Abstract

PURPOSE:To safely select the desired cells without receiving the influence of various bacteria in the external air by projecting laser light on a liquid suspension of the cells and discriminating the characteristics of the cells, then killing the cells by the laser light if the cells are not the desired cells. CONSTITUTION:The liquid suspension 2b of the cells and a sheath liquid 3 are injected into an inflow chamber 12a and the cells in the suspension 2b are admitted by each piece in an array shape into an orifice 12b. Fluorescence, forward scattered light and side scattered light are released from the cell when the laser light radiated from a laser oscillator 4 for measurement is projected on the above-mentioned cell. This light is detected by a photomultiplier 5 and the characteristic of the cell is discriminated by a control section 11b. The control section 11b compares the characteristics with preset reference values in accordance with the result of the discrimination and opens a shutter 14a of the laser oscillator 14 to project the laser light on said cell and to kill the same if the cell is not the cell to be selected. Only the desired cell is thus selected and recovered into a capturing device.
JP23170887A 1987-09-16 1987-09-16 Sell selection device Pending JPS6474451A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP23170887A JPS6474451A (en) 1987-09-16 1987-09-16 Sell selection device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP23170887A JPS6474451A (en) 1987-09-16 1987-09-16 Sell selection device

Publications (1)

Publication Number Publication Date
JPS6474451A true JPS6474451A (en) 1989-03-20

Family

ID=16927764

Family Applications (1)

Application Number Title Priority Date Filing Date
JP23170887A Pending JPS6474451A (en) 1987-09-16 1987-09-16 Sell selection device

Country Status (1)

Country Link
JP (1) JPS6474451A (en)

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008104432A (en) * 2006-10-27 2008-05-08 Mitsui Eng & Shipbuild Co Ltd Flow cytometer having cell sorting and treating function and method for sorting and treating live cell
JP2008530985A (en) * 2005-02-01 2008-08-14 アリックス インコーポレイテッド Cell sorting method and apparatus
EP2063988B1 (en) * 2006-09-21 2016-01-13 The University Court of the University of St. Andrews Optical sorting of cells
US9453849B2 (en) 2005-02-01 2016-09-27 Premium Genetics (Uk) Limited Methods for staining cells for identification and sorting
US11187224B2 (en) 2013-07-16 2021-11-30 Abs Global, Inc. Microfluidic chip
US11193879B2 (en) 2010-11-16 2021-12-07 1087 Systems, Inc. Use of vibrational spectroscopy for microfluidic liquid measurement
US11243494B2 (en) 2002-07-31 2022-02-08 Abs Global, Inc. Multiple laminar flow-based particle and cellular separation with laser steering
US11331670B2 (en) 2018-05-23 2022-05-17 Abs Global, Inc. Systems and methods for particle focusing in microchannels
US11415503B2 (en) 2013-10-30 2022-08-16 Abs Global, Inc. Microfluidic system and method with focused energy apparatus
US11628439B2 (en) 2020-01-13 2023-04-18 Abs Global, Inc. Single-sheath microfluidic chip
US11889830B2 (en) 2019-04-18 2024-02-06 Abs Global, Inc. System and process for continuous addition of cryoprotectant

Cited By (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11422504B2 (en) 2002-07-31 2022-08-23 Abs Global, Inc. Multiple laminar flow-based particle and cellular separation with laser steering
US11243494B2 (en) 2002-07-31 2022-02-08 Abs Global, Inc. Multiple laminar flow-based particle and cellular separation with laser steering
US11415936B2 (en) 2002-07-31 2022-08-16 Abs Global, Inc. Multiple laminar flow-based particle and cellular separation with laser steering
JP2008530985A (en) * 2005-02-01 2008-08-14 アリックス インコーポレイテッド Cell sorting method and apparatus
JP2012013714A (en) * 2005-02-01 2012-01-19 Arryx Inc Sensor
US9453849B2 (en) 2005-02-01 2016-09-27 Premium Genetics (Uk) Limited Methods for staining cells for identification and sorting
EP2063988B1 (en) * 2006-09-21 2016-01-13 The University Court of the University of St. Andrews Optical sorting of cells
JP2008104432A (en) * 2006-10-27 2008-05-08 Mitsui Eng & Shipbuild Co Ltd Flow cytometer having cell sorting and treating function and method for sorting and treating live cell
US11965816B2 (en) 2010-11-16 2024-04-23 1087 Systems, Inc. Use of vibrational spectroscopy for microfluidic liquid measurement
US11193879B2 (en) 2010-11-16 2021-12-07 1087 Systems, Inc. Use of vibrational spectroscopy for microfluidic liquid measurement
US11512691B2 (en) 2013-07-16 2022-11-29 Abs Global, Inc. Microfluidic chip
US11187224B2 (en) 2013-07-16 2021-11-30 Abs Global, Inc. Microfluidic chip
US11415503B2 (en) 2013-10-30 2022-08-16 Abs Global, Inc. Microfluidic system and method with focused energy apparatus
US11639888B2 (en) 2013-10-30 2023-05-02 Abs Global, Inc. Microfluidic system and method with focused energy apparatus
US11796449B2 (en) 2013-10-30 2023-10-24 Abs Global, Inc. Microfluidic system and method with focused energy apparatus
US11331670B2 (en) 2018-05-23 2022-05-17 Abs Global, Inc. Systems and methods for particle focusing in microchannels
US11889830B2 (en) 2019-04-18 2024-02-06 Abs Global, Inc. System and process for continuous addition of cryoprotectant
US11628439B2 (en) 2020-01-13 2023-04-18 Abs Global, Inc. Single-sheath microfluidic chip

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