JPS642591A - Production of alkaloid - Google Patents

Production of alkaloid

Info

Publication number
JPS642591A
JPS642591A JP63064650A JP6465088A JPS642591A JP S642591 A JPS642591 A JP S642591A JP 63064650 A JP63064650 A JP 63064650A JP 6465088 A JP6465088 A JP 6465088A JP S642591 A JPS642591 A JP S642591A
Authority
JP
Japan
Prior art keywords
alkaloid
hairy roots
roots
plasmid
solanaceous plant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP63064650A
Other languages
Japanese (ja)
Other versions
JPH012591A (en
Inventor
Hiroshi Kamata
Hitoshi Saga
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Lion Corp
Original Assignee
Lion Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lion Corp filed Critical Lion Corp
Publication of JPS642591A publication Critical patent/JPS642591A/en
Publication of JPH012591A publication Critical patent/JPH012591A/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/18—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
    • C12P17/188—Heterocyclic compound containing in the condensed system at least one hetero ring having nitrogen atoms and oxygen atoms as the only ring heteroatoms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/04—Plant cells or tissues
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/18—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
    • C12P17/182—Heterocyclic compounds containing nitrogen atoms as the only ring heteroatoms in the condensed system
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/91—Cell lines ; Processes using cell lines

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Nutrition Science (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

PURPOSE:To efficiently produce an alkaloid, by transforming solanaceous plant cells with an Ri plasmid belonging to the genus Agrobacterium and cultivating the resultant hairy roots. CONSTITUTION:Part (T-DNA of an Ri plasmid of a microorganism Agrobacterium rhizogenes is introduced into stems, roots, leaves, etc., of a solanaceous plant to provide transformed hairy roots. The above-mentioned hairy roots in an amount of about 10mg-1g (fresh weight) are then inoculated into 50ml liquid culture medium containing 0.1muM-1,000mM inorganic component, 1-120g/l carbon source and 0.01-10muM plant growth regulator substance and 0.1-100mg/l each of vitamins and amino acids and continuously cultivated at 10-35 deg.C, especially 23-28 deg.C by a batch or column method, etc., to recover the aimed alkaloid from the obtained culture.
JP63-64650A 1987-03-20 1988-03-17 Method for producing alkaloids Pending JPH012591A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP62-66787 1987-03-20
JP6678787 1987-03-20

Publications (2)

Publication Number Publication Date
JPS642591A true JPS642591A (en) 1989-01-06
JPH012591A JPH012591A (en) 1989-01-06

Family

ID=

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5212528A (en) * 1990-11-07 1993-05-18 Sato Corporation Fixing device and method which uses a heat dissipating roller having fins

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5212528A (en) * 1990-11-07 1993-05-18 Sato Corporation Fixing device and method which uses a heat dissipating roller having fins

Similar Documents

Publication Publication Date Title
Uwajima et al. Isolation and Crystallization of Extracellular 3 β-Hydroxysteroid Oxidase of Brevibacterium sterolicum nov. sp.
JPS56144093A (en) Preparation of l-proline by fermentation
EP0195944A3 (en) Process for the continuous preparation of l-carnitine
JPS5736991A (en) Preparation of 2,5-diketo-d-gluconic acid
JPS5763080A (en) Preparation of bacterial cell having high nitrilase activity
Noparatnaraporn et al. Stimulation of vitamin B12 formation in aerobically-grown Rhodopseudomonas gelatinosa under microaerobic condition
JPS642591A (en) Production of alkaloid
JPS5688799A (en) Preparation of l-lysine
JPS56160996A (en) Preparation of abscisic acid by fermentation method
EP0317512A3 (en) Productive method for the regeneration of cotton from cultivated cells
JPS5675097A (en) Thermophilic aspartase and its preparation
JPS52125686A (en) Process for growing yeast in high yield
JPS56154990A (en) Preparation of l-aminoacid oxidase
Lechat et al. Gambierdiscus toxicus: culture and toxin production
JPS57110192A (en) Preparation of l-lactic acid
JPS5675095A (en) Preparation of heat-resistant acetic acid kinase
JPS6474999A (en) Production of optically active (r)-(-)-beta-hydroxylactic acid
JPS54145280A (en) Production of cells of photosynthetic bacterium
JPS572684A (en) Preparation of amylase inhibitor ai-b
JPS55162994A (en) Preparation of fructose
JPS6430594A (en) Production of anthocyan dyestuff by tissue culture of callus of roselle
JPS5743684A (en) Preparation of maltose dehydrogenase
JPS56124381A (en) Preparation of uricase by fermentation method
JPS56158092A (en) Preparation of heat-resistant glucokinase
JPS5729289A (en) Preparation of l-lysine by fermentation method