JPS6374479A - Foam for cultivation of microorganism - Google Patents
Foam for cultivation of microorganismInfo
- Publication number
- JPS6374479A JPS6374479A JP61222480A JP22248086A JPS6374479A JP S6374479 A JPS6374479 A JP S6374479A JP 61222480 A JP61222480 A JP 61222480A JP 22248086 A JP22248086 A JP 22248086A JP S6374479 A JPS6374479 A JP S6374479A
- Authority
- JP
- Japan
- Prior art keywords
- foam
- culture
- medium component
- matrix
- microorganisms
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000006260 foam Substances 0.000 title claims abstract description 105
- 244000005700 microbiome Species 0.000 title claims abstract description 32
- 239000012533 medium component Substances 0.000 claims abstract description 36
- 239000011159 matrix material Substances 0.000 claims abstract description 25
- 238000012258 culturing Methods 0.000 claims abstract description 17
- 239000000203 mixture Substances 0.000 claims abstract description 12
- 238000005187 foaming Methods 0.000 claims abstract description 11
- 229920005830 Polyurethane Foam Polymers 0.000 claims abstract description 8
- 239000011496 polyurethane foam Substances 0.000 claims abstract description 8
- -1 polyethylene Polymers 0.000 claims description 7
- 229920000728 polyester Polymers 0.000 claims description 6
- 229920006327 polystyrene foam Polymers 0.000 claims description 4
- 239000004698 Polyethylene Substances 0.000 claims description 3
- BZHJMEDXRYGGRV-UHFFFAOYSA-N Vinyl chloride Chemical compound ClC=C BZHJMEDXRYGGRV-UHFFFAOYSA-N 0.000 claims description 3
- 229920001477 hydrophilic polymer Polymers 0.000 claims description 3
- 229920000573 polyethylene Polymers 0.000 claims description 3
- 239000012948 isocyanate Substances 0.000 abstract description 11
- 239000004721 Polyphenylene oxide Substances 0.000 abstract description 8
- 229920000570 polyether Polymers 0.000 abstract description 8
- 150000002513 isocyanates Chemical class 0.000 abstract description 7
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 abstract description 4
- 239000004088 foaming agent Substances 0.000 abstract description 4
- 239000008103 glucose Substances 0.000 abstract description 4
- 229940041514 candida albicans extract Drugs 0.000 abstract description 2
- 238000010438 heat treatment Methods 0.000 abstract description 2
- 239000012138 yeast extract Substances 0.000 abstract description 2
- 238000011109 contamination Methods 0.000 abstract 1
- 238000012136 culture method Methods 0.000 description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 11
- 239000002609 medium Substances 0.000 description 8
- 239000000047 product Substances 0.000 description 8
- 239000000243 solution Substances 0.000 description 8
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 6
- 229930182555 Penicillin Natural products 0.000 description 6
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 6
- 238000000034 method Methods 0.000 description 6
- 229940049954 penicillin Drugs 0.000 description 6
- 238000006243 chemical reaction Methods 0.000 description 5
- 239000000284 extract Substances 0.000 description 5
- 239000007787 solid Substances 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 4
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 4
- 239000007789 gas Substances 0.000 description 4
- 239000008101 lactose Substances 0.000 description 4
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 4
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 description 4
- 241000233866 Fungi Species 0.000 description 3
- 241000382353 Pupa Species 0.000 description 3
- 240000008042 Zea mays Species 0.000 description 3
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 3
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 3
- 239000007864 aqueous solution Substances 0.000 description 3
- 235000005822 corn Nutrition 0.000 description 3
- 229920001971 elastomer Polymers 0.000 description 3
- IQPQWNKOIGAROB-UHFFFAOYSA-N isocyanate group Chemical group [N-]=C=O IQPQWNKOIGAROB-UHFFFAOYSA-N 0.000 description 3
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 3
- 235000019341 magnesium sulphate Nutrition 0.000 description 3
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 3
- 235000019796 monopotassium phosphate Nutrition 0.000 description 3
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 3
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 2
- 239000004604 Blowing Agent Substances 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- 241000254173 Coleoptera Species 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- 108010010803 Gelatin Proteins 0.000 description 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- IMNFDUFMRHMDMM-UHFFFAOYSA-N N-Heptane Chemical compound CCCCCCC IMNFDUFMRHMDMM-UHFFFAOYSA-N 0.000 description 2
- OFBQJSOFQDEBGM-UHFFFAOYSA-N Pentane Chemical compound CCCCC OFBQJSOFQDEBGM-UHFFFAOYSA-N 0.000 description 2
- 241000254062 Scarabaeidae Species 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 244000309466 calf Species 0.000 description 2
- 239000004202 carbamide Substances 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 239000001569 carbon dioxide Substances 0.000 description 2
- 229910002092 carbon dioxide Inorganic materials 0.000 description 2
- 239000003925 fat Substances 0.000 description 2
- 235000013312 flour Nutrition 0.000 description 2
- 229920000159 gelatin Polymers 0.000 description 2
- 239000008273 gelatin Substances 0.000 description 2
- 235000019322 gelatine Nutrition 0.000 description 2
- 235000011852 gelatine desserts Nutrition 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 230000000749 insecticidal effect Effects 0.000 description 2
- 235000013372 meat Nutrition 0.000 description 2
- 239000012092 media component Substances 0.000 description 2
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 2
- 238000009629 microbiological culture Methods 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 239000003921 oil Substances 0.000 description 2
- 235000019198 oils Nutrition 0.000 description 2
- 229910052697 platinum Inorganic materials 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 239000004317 sodium nitrate Substances 0.000 description 2
- 235000010344 sodium nitrate Nutrition 0.000 description 2
- 230000003068 static effect Effects 0.000 description 2
- FDYWJVHETVDSRA-UHFFFAOYSA-N 1,1-diisocyanatobutane Chemical compound CCCC(N=C=O)N=C=O FDYWJVHETVDSRA-UHFFFAOYSA-N 0.000 description 1
- ZXHZWRZAWJVPIC-UHFFFAOYSA-N 1,2-diisocyanatonaphthalene Chemical compound C1=CC=CC2=C(N=C=O)C(N=C=O)=CC=C21 ZXHZWRZAWJVPIC-UHFFFAOYSA-N 0.000 description 1
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- PAWQVTBBRAZDMG-UHFFFAOYSA-N 2-(3-bromo-2-fluorophenyl)acetic acid Chemical compound OC(=O)CC1=CC=CC(Br)=C1F PAWQVTBBRAZDMG-UHFFFAOYSA-N 0.000 description 1
- UPMLOUAZCHDJJD-UHFFFAOYSA-N 4,4'-Diphenylmethane Diisocyanate Chemical compound C1=CC(N=C=O)=CC=C1CC1=CC=C(N=C=O)C=C1 UPMLOUAZCHDJJD-UHFFFAOYSA-N 0.000 description 1
- 241000251468 Actinopterygii Species 0.000 description 1
- 102000009027 Albumins Human genes 0.000 description 1
- 108010088751 Albumins Proteins 0.000 description 1
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
- 241000223679 Beauveria Species 0.000 description 1
- 241000751139 Beauveria bassiana Species 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 239000004372 Polyvinyl alcohol Substances 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 235000019270 ammonium chloride Nutrition 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 1
- 239000004305 biphenyl Substances 0.000 description 1
- 235000010290 biphenyl Nutrition 0.000 description 1
- 238000007664 blowing Methods 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- 239000000306 component Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- PXBRQCKWGAHEHS-UHFFFAOYSA-N dichlorodifluoromethane Chemical compound FC(F)(Cl)Cl PXBRQCKWGAHEHS-UHFFFAOYSA-N 0.000 description 1
- 235000019404 dichlorodifluoromethane Nutrition 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 229940096118 ella Drugs 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 238000005470 impregnation Methods 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000002917 insecticide Substances 0.000 description 1
- 238000004898 kneading Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 239000013028 medium composition Substances 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 235000013379 molasses Nutrition 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 239000003208 petroleum Substances 0.000 description 1
- ZUOUZKKEUPVFJK-UHFFFAOYSA-N phenylbenzene Natural products C1=CC=CC=C1C1=CC=CC=C1 ZUOUZKKEUPVFJK-UHFFFAOYSA-N 0.000 description 1
- 235000021317 phosphate Nutrition 0.000 description 1
- 150000003013 phosphoric acid derivatives Chemical class 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 230000000379 polymerizing effect Effects 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 229920002451 polyvinyl alcohol Polymers 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 238000000197 pyrolysis Methods 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 235000012424 soybean oil Nutrition 0.000 description 1
- 239000003549 soybean oil Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- DVKJHBMWWAPEIU-UHFFFAOYSA-N toluene 2,4-diisocyanate Chemical compound CC1=CC=C(N=C=O)C=C1N=C=O DVKJHBMWWAPEIU-UHFFFAOYSA-N 0.000 description 1
- OOLLAFOLCSJHRE-ZHAKMVSLSA-N ulipristal acetate Chemical compound C1=CC(N(C)C)=CC=C1[C@@H]1C2=C3CCC(=O)C=C3CC[C@H]2[C@H](CC[C@]2(OC(C)=O)C(C)=O)[C@]2(C)C1 OOLLAFOLCSJHRE-ZHAKMVSLSA-N 0.000 description 1
- 239000008096 xylene Substances 0.000 description 1
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- General Preparation And Processing Of Foods (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
【発明の詳細な説明】
（産業上の利用分野）
本発明は微生物の培養が効果的になされ得る微生物培養
用発泡体に関する。DETAILED DESCRIPTION OF THE INVENTION (Industrial Field of Application) The present invention relates to a foam for culturing microorganisms that can effectively culture microorganisms.
（従来の技術）
微生物の培養には、液内培養法のほかに、米ふすまなど
を用いる固体培養法がある。目的生産物（例えば、菌体
９代謝生産物など）の種類により。(Prior Art) In addition to the submerged culture method, microorganisms can be cultured using a solid culture method using rice bran or the like. Depending on the type of target product (for example, bacterial cell 9 metabolic products, etc.).
液内培養法や固体培養法が単独で、あるいは組み合わせ
て用いられている。しかし、液内培養法では、培養によ
りベレットが形成され、それにより微生物の培養効率が
低下する。固体培養法では微生物により生産された生産
物の分離が困難である。Submerged culture methods and solid state culture methods are used alone or in combination. However, in the submerged culture method, pellets are formed during culture, which reduces the culture efficiency of microorganisms. With solid state culture methods, it is difficult to separate products produced by microorganisms.
このような欠点を解決するために１発泡体に培地を担持
させ、培養を行う方法が提案されている。In order to solve these drawbacks, a method has been proposed in which a single foam is made to carry a medium and culture is carried out.
例えば、特公昭５５−３６３１３号公報には、スポンジ
などの発泡体に培地液を含浸した後、静置培養する方法
が開示されている。しかし、この方法では。For example, Japanese Patent Publication No. 55-36313 discloses a method in which a foam such as a sponge is impregnated with a culture medium and then cultured stationary. But with this method.
発泡体への培地液の含浸が困難である。しかも。It is difficult to impregnate the foam with the medium solution. Moreover.
含浸量には限界があり、市販のポリウレタン発泡体で３
０〜５０％、含浸性の良好な発泡体でも９０％までであ
る。従って、微生物に対し充分な培地が供給され得ず、
微生物の培養が効果的になされない。There is a limit to the amount of impregnation, and commercially available polyurethane foam has a limit of 3.
0 to 50%, and up to 90% even for foams with good impregnability. Therefore, sufficient culture medium cannot be supplied to microorganisms,
Microorganisms are not cultured effectively.
このような発泡体は、保水力に乏しく乾燥しやすいため
、微生物の培養に不適である。液内培養法において、多
数の発泡体片を添加する培養方法（特開昭６０−２１４
８７８号公報に開示）や分子中にベブタイドマトリソク
スが形成された親水性発泡体（特公昭５３−１１３１６
号公報に開示）も知られている。Such foams have poor water retention capacity and tend to dry out, making them unsuitable for culturing microorganisms. In the submerged culture method, a culture method in which a large number of foam pieces are added (Japanese Patent Application Laid-Open No. 60-214
No. 878) and a hydrophilic foam in which bebutide matrix is formed in the molecule (Japanese Patent Publication No. 53-11316)
(disclosed in Japanese Patent Publication No. 1) is also known.
しかし、これらの発泡体では、培地成分中の固形分や油
脂が発泡体に吸着されるため、培地が発泡体の内部にま
で浸透しない。従って、微生物は発泡体の表面でのみ培
養されるため、微生物の培養効率が低い。However, in these foams, the solid content and fats and oils in the medium components are adsorbed by the foam, so the medium does not penetrate into the inside of the foam. Therefore, since microorganisms are cultured only on the surface of the foam, the efficiency of culturing microorganisms is low.
（発明が解決しようとする問題点）
本発明は上記従来の問題点を解決するものであり、その
目的とするところは、微生物の培養が効果的になされ得
る微生物培養用発泡体を提供することにある。本発明の
他の目的は、微生物の培養により得られた生産物の分離
が容易な微生物培養用発泡体を提供することにある。(Problems to be Solved by the Invention) The present invention solves the above conventional problems, and its purpose is to provide a foam for culturing microorganisms that can effectively culture microorganisms. It is in. Another object of the present invention is to provide a foam for culturing microorganisms that allows easy separation of products obtained by culturing microorganisms.
（問題点を解決するための手段）
本発明は２発泡体に培地液を含浸させる従来の方法に代
えて１発泡体組成物に培地成分を混合し発泡させること
により１発泡体マトリックス内に培地成分が組み込まれ
るため２発泡体表面だけでなく内部での培養がなされ得
、それにより、微生物の培養が効果的に行われ得る。と
の発明者の知見にもとづいて完成された。(Means for Solving the Problems) The present invention provides a medium solution in one foam matrix by mixing and foaming medium components in one foam composition, instead of the conventional method of impregnating two foams with a medium solution. Due to the incorporation of components, cultivation can occur not only on the surface of the foam, but also inside the foam, thereby allowing effective cultivation of microorganisms. It was completed based on the inventor's knowledge.
本発明の微生物培養用発泡体は１発泡体組成物を培地成
分とともに発泡させて得られ、そして発泡体マトリック
ス内に培地成分を含有し、そのごとにより上記目的が達
成される。The microorganism culture foam of the present invention is obtained by foaming a foam composition with a medium component and contains the medium component within the foam matrix, thereby achieving the above objectives.
培地成分は１発泡体組成物の発泡の際に、主として発泡
体マトリックス内に物理的に組み込まれる。しかし１例
えば、培地成分がアミノ基、カルボキシル基を有し２発
泡体組成物がイソシアネート基を有する場合には、培地
成分と発泡体マトリックスとが化学的に反応する。それ
により、培地成分が尿素結合や酸アミド結合により１発
泡体マトリックス内に化学結合で担持される。培地成分
の水酸基は、イソシアネート基と反応して炭酸ガスを発
生し１発泡を促進する。The medium components are primarily physically incorporated into the foam matrix during foaming of a foam composition. However, if, for example, the medium component has amino or carboxyl groups and the foam composition has isocyanate groups, the medium component and the foam matrix will chemically react. Thereby, the medium components are chemically supported within the foam matrix by urea bonds and acid amide bonds. The hydroxyl groups of the medium components react with the isocyanate groups to generate carbon dioxide gas and promote foaming.
発泡体マトリックスには２例えば、ポリウレタンフォー
ム、ポリスチレン発泡体、塩化ヒニル発泡体、ポリエチ
レン発泡体、ポリエステル発泡体がある。特にポリウレ
タンフォームが好ましい。Foam matrices include, for example, polyurethane foam, polystyrene foam, vinyl chloride foam, polyethylene foam, and polyester foam. Particularly preferred is polyurethane foam.
ポリウレタンフォームは、ポリエーテルまたはポリエス
テルと９分子内に２個以上のイソシアネート基を有する
イソシアネート化合物と、水や他の発泡剤とを反応させ
発泡させて得られる。イソシアネート化合物としては９
通常の多官能イソシアネートが用いられ２例えば、トリ
レンジイソシアネート ジフェニルメタンジイソシアネ
ート。Polyurethane foam is obtained by reacting polyether or polyester, an isocyanate compound having two or more isocyanate groups in nine molecules, and water or other foaming agent, and then foaming the polyether or polyester. 9 as an isocyanate compound
Common polyfunctional isocyanates are used, such as tolylene diisocyanate, diphenylmethane diisocyanate.
ジフェニルジイソシアネートナフタリンジイソシアネー
トキシレンジイソシアネート、ブタンジイソシアネート
−、トリフェニルメタン−４，４”、４″−トリイソシ
アネートがある。ポリエーテルまたはポリエステルはイ
ソシアネート化合物と反応してプレポリマーとされ、こ
のプレポリマーと水とを反応させることにより、炭酸ガ
スが発生して発泡し、ポリウレタンフォームが形成され
る。ポリスチレン発泡体は、ポリスチレンプレポリマー
に発泡剤（ペンタン、ヘキサン、ヘプタンなど）を加え
、水中乳化重合により形成される。塩化ビニル発泡体は
、熱分解法やガス吹き込み法により得られる。ポリスチ
レン発泡体は、ポリエチレンプレポリマーに石油エーテ
ル、ガスフレオン１２などの発泡剤を加え、混練、加熱
発泡させて得られる。Examples include diphenyl diisocyanate, naphthalene diisocyanate, xylene diisocyanate, butane diisocyanate, and triphenylmethane-4,4'', 4''-triisocyanate. Polyether or polyester is reacted with an isocyanate compound to form a prepolymer, and by reacting this prepolymer with water, carbon dioxide gas is generated and foamed to form polyurethane foam. Polystyrene foam is formed by adding a blowing agent (pentane, hexane, heptane, etc.) to a polystyrene prepolymer and emulsion polymerizing it in water. Vinyl chloride foam can be obtained by a pyrolysis method or a gas blowing method. A polystyrene foam is obtained by adding a foaming agent such as petroleum ether or gas Freon 12 to a polyethylene prepolymer, kneading the mixture, and foaming the mixture by heating.
いずれの発泡体を用いる場合でも１発泡前のプレポリマ
ーに対し発泡剤とともに培地成分を加えて発泡させるこ
とにより、培地成分が発泡体マトリックス内に組み込ま
れる。No matter which foam is used, the medium component is incorporated into the foam matrix by adding the medium component together with a foaming agent to the prepolymer before foaming and foaming.
培地成分には、同化可能な炭素源と同化可能な窒素源に
無機塩類および天然有機物が含有される。Media components include an assimilable carbon source, an assimilable nitrogen source, inorganic salts, and natural organic matter.
炭素源には９例えば、グルコース、サッカロース。Examples of carbon sources include glucose and sucrose.
ラクトース、マルトース、グリセリン、デンプン。Lactose, maltose, glycerin, starch.
糖蜜がある。窒素源には１例えば、硫酸アンモニウム、
塩化アンモニウム、硝酸アンモニウムがある。無機塩類
には１例えば、リン酸二水素カリウムなどのリン酸塩、
硫酸マグネシウム、マグネシウム、カリウム、カルシウ
ムがある。天然有機物には２例えば、肉エキス、魚肉抽
出液、サナギ粉などの動物組織抽出物または粉砕物；コ
ーンスチープリカー、大豆油、麦芽エキス、大豆粉など
の植物組織抽出物または粉砕物；乾燥酵母、酵母エキス
、ポリペプトンなどの微生物菌体またはその抽出物があ
る。There's molasses. Nitrogen sources include 1, for example, ammonium sulfate,
There are ammonium chloride and ammonium nitrate. Inorganic salts include 1. For example, phosphates such as potassium dihydrogen phosphate,
Contains magnesium sulfate, magnesium, potassium, and calcium. Natural organic substances include 2 For example, animal tissue extracts or ground products such as meat extract, fish meat extract, and pupa powder; plant tissue extracts or ground products such as corn steep liquor, soybean oil, malt extract, and soy flour; dried yeast. , yeast extract, polypeptone, and other microbial cells or their extracts.
発泡体マトリックスがポリウレタンフォームであれば、
プレポリマー（ポリエーテルまたはポリエステルとイソ
シアネート化合物との反応物）と水や他の発泡剤に培地
成分が加えられ１反応に供される。水溶性の培地成分は
水溶液にしてプレポリマーと混合される。水不溶性の培
地成分は、プレポリマーの水溶液に分散される。水の量
は、プレポリマー１００重量部に対し、１０〜１００重
量部の範囲が好ましい。１０重量部を下まわると９発泡
反応が遅延し、所望の発泡密度の発泡体が得られない。If the foam matrix is polyurethane foam,
Medium components are added to a prepolymer (a reaction product of polyether or polyester and an isocyanate compound), water and other blowing agents, and the mixture is subjected to one reaction. Water-soluble medium components are mixed with the prepolymer in an aqueous solution. The water-insoluble medium components are dispersed in the aqueous solution of the prepolymer. The amount of water is preferably in the range of 10 to 100 parts by weight based on 100 parts by weight of the prepolymer. If it is less than 10 parts by weight, the foaming reaction will be delayed and a foam with the desired foam density will not be obtained.
プレポリマーと培地成分との反応や発泡体マトリックス
中への培地成分の担持も充分になされない。１００重量
部を上まわると、水とプレポリマーとの反応が優先して
培地成分が発泡体マトリックス内に取り込まれにくい。The reaction between the prepolymer and the medium components and the support of the medium components into the foam matrix are also insufficient. When the amount exceeds 100 parts by weight, the reaction between water and prepolymer takes priority and the medium components are difficult to be incorporated into the foam matrix.
培地成分は、プレポリマー１００重量部に対し、２０〜
５００重量部、好ましくは５０〜２００重量部とされる
。２０重量部を下まわると、培地成分が発泡体マトリッ
クス内に充分に含有されない。５００重量部を上まわる
量の培地成分は発泡体マトリックス内に担持され得ない
。The medium component is 20 to 100 parts by weight of the prepolymer.
The amount is 500 parts by weight, preferably 50 to 200 parts by weight. Below 20 parts by weight, the medium components are not sufficiently contained within the foam matrix. Amounts of media components exceeding 500 parts by weight cannot be supported within the foam matrix.
本発明の発泡体には１発泡体マトリックスに尿素結合や
酸アミド結合を形成させ２強靭なベプタイドマトリソク
スを得るために、可溶性コラーゲン、ゼラチン、アルブ
ミンなどのベプタイドを添加してもよい。発泡体マトリ
ックスには、保水力を上げるべ（、好ましくは親水性ポ
リマーが含有される。親水性ポリマーの含有により１発
泡体マトリックスへの水分の補給がほとんど必要でなく
なる。親水性ポリマーには３例えば、寒天、ポリビニル
アルコール、ポリアクリルアミドがある。Veptides such as soluble collagen, gelatin, albumin, etc. may be added to the foam of the present invention in order to (1) form urea bonds and acid amide bonds in the foam matrix and (2) obtain a strong peptide matrix. The foam matrix preferably contains a hydrophilic polymer to increase its water retention capacity.The inclusion of the hydrophilic polymer reduces the need for water replenishment to the foam matrix. Examples include agar, polyvinyl alcohol, and polyacrylamide.
このように得られた微生物培養用発泡体は、適当量の水
分を含有させた後、オートクレーブ（１２０’Ｃ，１，
２ａｔｍ）などにより滅菌して静置培養に供される。こ
の発泡体を粉砕した後、培地成分および水とともに液体
内培養に供してもよい。また２発泡体に殺虫性微生物を
接種して発泡体内にて培養し、殺虫剤として用いること
も可能である。例えば、カミキリムシ、コガネムシの幼
虫などの殺虫微生物であるＢｅａｕｖｅｒｉａ ｔｅｎ
ｅｌｌａを培養してカミキリムシ、コガネムシの幼虫な
どの害虫駆除に用いられる。この発泡体は、１１０℃、
１時間以上の乾燥により発泡体中の水分を除去すれば、
無菌的に長期間保存し得る。使用時には含水させればよ
い。The thus obtained foam for microbial culture was added with an appropriate amount of water and then placed in an autoclave (120'C, 1,
2 atm), etc., and subjected to static culture. After the foam is crushed, it may be subjected to submerged culture together with medium components and water. It is also possible to inoculate insecticidal microorganisms into two foams, culture them within the foams, and use them as insecticides. For example, Beauveria ten, which is an insecticidal microorganism that kills longhorn beetles, scarab beetle larvae, etc.
ella is cultivated and used to exterminate pests such as longhorn beetles and scarab beetle larvae. This foam is heated at 110°C.
If the moisture in the foam is removed by drying for over 1 hour,
Can be stored aseptically for a long period of time. It may be hydrated when used.
（実施例） 以下に本発明を実施例について述べる。(Example) The present invention will be described below with reference to examples.
尖旌±士 ポリエーテルイソシアネート（ソフランネート。expert Polyether isocyanate (sofuranate).
東洋ゴム社製）１００重量部に対し、培地成分としてグ
ルコース１５重量部、サナギ粉３０重量部および寒天１
１重量部を室温で混合し、さらにゼラチンの５％水溶液
２０重量部を混合して室温で数分間反応させた。反応に
より１発泡体マトリックス内に培地成分を含有する微生
物培養用発泡体が得られた。(manufactured by Toyo Rubber Co., Ltd.), 15 parts by weight of glucose, 30 parts by weight of pupa powder, and 1 part of agar as medium components.
1 part by weight was mixed at room temperature, and further mixed with 20 parts by weight of a 5% gelatin aqueous solution, and reacted at room temperature for several minutes. The reaction yielded a microbial culture foam containing medium components within one foam matrix.
得られた発泡体をオートクレーブ（１２０”Ｃ，１，２
ａｔｍ）で２０分間滅菌した。The obtained foam was autoclaved (120"C, 1,2
ATM) for 20 minutes.
糸状菌（Ｂｅａｕｖｅｒｉａ ｔｅｎｅｌｌａ）を、グ
ルコース２゜ｇおよびサナギ粉４０ｇ／ｌから抽出して
得た培地１００−を用いて、３００−三角フラスコ中に
てロータリーシェーカーで振盪しながら前培養した。A filamentous fungus (Beauveria tenella) was precultured in a 300-Erlenmeyer flask with 100-ml of medium extracted from 2°g of glucose and 40 g/l of pupa flour with shaking on a rotary shaker.
この培養液３−を上記滅菌発泡体（３Ｑｃ＋Ｊ Ｘ １
ｃｍ ）に接種し、静置培養した。２５°Ｃで１週間
培養した後２発泡体を観察したところ、糸状菌の胞子が
発泡体の全面をおおっていた。This culture solution 3- was transferred to the above sterilized foam (3Qc+J
cm) and statically cultured. When the two foams were observed after culturing at 25°C for one week, the entire surface of the foams was covered with spores of filamentous fungi.
培養液を接種する前の発泡体に５−または１０ ｍｌの
水を含浸させて同様の静置培養を行なったところ、いず
れも発泡体の全面に胞子が存在した。When the foam before inoculation with the culture solution was impregnated with 5 or 10 ml of water and the same static culture was carried out, spores were present on the entire surface of the foam.
ル較拠上
培地成分を加えなかったこと以外は、実施例１と同様の
方法により微生物培養用発泡体を得た。A foam for culturing microorganisms was obtained in the same manner as in Example 1, except that no medium components were added.
この発泡体（３０ｃｏｌ Ｘ ｌ ｃｍ ）各３個に対
し、実施例１の液体培地を２．５ｍｌ、 ５ ｍｌ、
ｌＱｍｌ含浸させ、同様に培養液３ ｍｌを接種して
培養した。For each three of these foams (30 col x l cm), 2.5 ml, 5 ml, and 5 ml of the liquid medium of Example 1 were added.
The cells were impregnated with 1Qml, and 3ml of the culture solution was similarly inoculated and cultured.
２５℃で１週間培養した後１発泡体を観察したところ、
含浸量が１０−の発泡体は糸状菌の胞子が発泡体の２／
３以上をおおっていたものの、他の発泡体では、培養液
の少ない部分には菌糸が生育しておらず、培養むらが認
められた。When one foam was observed after culturing at 25°C for one week,
For foams with an impregnated amount of 10, the spores of filamentous fungi are 2/2 of the foam.
However, in other foams, mycelium did not grow in areas with less culture solution, and uneven culture was observed.
去隻開叉 ポリエーテルイソシアネート（ソフランネート。departure ship fork Polyether isocyanate (sofuranate).
東洋ゴム社製）１００重量部に、ラクトース１５０重量
部、硝酸ナトリウム１１．２５重量部、リン酸二水素カ
リウム１８．７５重量部、硫酸マグネシウム９．４０重
量部を加えて室温で混合した。そして、コーンメチ−プ
リカーフ５重量部を、上記イソシアネート混合物に加え
て室温で数分間反応させて培地成分を含有する発泡体を
得た。150 parts by weight of lactose, 11.25 parts by weight of sodium nitrate, 18.75 parts by weight of potassium dihydrogen phosphate, and 9.40 parts by weight of magnesium sulfate were added to 100 parts by weight of Toyo Rubber Co., Ltd.) and mixed at room temperature. Then, 5 parts by weight of corn methyl pre-calf was added to the above isocyanate mixture and reacted for several minutes at room temperature to obtain a foam containing medium components.
得られた発泡体を５ｆｉ角に細断した。この発泡体４．
９〜９．７ｇおよび蒸留水Ｌｏｏｓｇを３００＋＋＋＃
三角フラスコに入れ、 Ｐｅｎ、 ｃｈＨ並ＬｕＬ（
胞子）を１白金耳植菌してロータリーシェーカーにて培
養した。菌糸は発泡体内で生育してペニシリンを生産し
２発泡体の外に放出した。得られたペニシリン量は、特
開昭６０−２１４８７４の発泡体培養法を用いて生産さ
れるペニシリン量と同程度であった。培養７日目の培養
結果を第１表に示した。The obtained foam was shredded into 5fi square pieces. This foam 4.
9-9.7g and distilled water Loosg 300+++#
Pour into an Erlenmeyer flask, add Pen, chH and LuL (
One platinum loop of spores was inoculated and cultured in a rotary shaker. Mycelia grew within the foam and produced penicillin, which was released outside the foam. The amount of penicillin obtained was comparable to the amount of penicillin produced using the foam culture method of JP-A-60-214874. The culture results on the 7th day of culture are shown in Table 1.
実施班主 ポリエーテルイソシアネート（ソフランネート。Implementation team leader Polyether isocyanate (sofuranate).
東洋ゴム社製）１００重量部に、ラクトース０重量部、
硝酸ナトリウム１１．２５重量部、リン酸二水素カリウ
ム１８．７５重量部、硫酸マグネシウム９．４０重量部
を加えて室温で混合した。そして、コーンメチ−プリカ
ーフ５重量部を、上記イソシアネート混合物に加えて室
温で数分間反応させて、培地成分を含有する発泡体を得
た。Toyo Rubber Co., Ltd.) 100 parts by weight, 0 parts by weight of lactose,
11.25 parts by weight of sodium nitrate, 18.75 parts by weight of potassium dihydrogen phosphate, and 9.40 parts by weight of magnesium sulfate were added and mixed at room temperature. Then, 5 parts by weight of corn methyl pre-calf was added to the above isocyanate mixture and reacted for several minutes at room temperature to obtain a foam containing medium components.
得られた発泡体を５鰭角に細断した。この発泡体３〜６
ｇおよび４重量％ラクトース溶液１００ｍｆｆを３００
−三角フラスコに入れ、 Ｐｅｎ、 ｃｈ■赳■匹Ｌ
（胞子）を１白金耳植菌してロータリーシェーカーにて
培養した。菌糸は発泡体内で生育してペニシリンを生産
し２発泡体の外に放出した。得られたペニシリン量は、
特開昭６０−２１４８７８の発泡体培養法を用いて生産
されるペニシリン量と同程度であった。培養７日目の培
養結果を第２表に示した。The resulting foam was shredded into 5 fin angles. This foam 3-6
300 g and 100 mff of 4 wt% lactose solution
- Put in an Erlenmeyer flask, Pen, ch ■赳■ L
One platinum loop of (spores) was inoculated and cultured in a rotary shaker. Mycelia grew within the foam and produced penicillin, which was released outside the foam. The amount of penicillin obtained is
The amount of penicillin produced using the foam culture method of JP-A-60-214878 was comparable. The culture results on the 7th day of culture are shown in Table 2.
北較斑Ｉ
発泡体を用いず番ご通常の培地組成にて展ｃｈｒ ｓｏ
ｅｎｕｍ （胞子）を培養した。培養方法は。Northern Spot I Expanded in normal medium composition without using foam.
enum (spores) were cultured. What is the culture method?
実施例２と同様とし、結果を第３表に示した。The procedure was the same as in Example 2, and the results are shown in Table 3.
（発明の効果） 本発明の微生物培養用発泡体は、このように。(Effect of the invention) The foam for culturing microorganisms of the present invention is thus produced.
発泡体マトリックス内に培地成分が含有されているため
９発泡体の表面だけではなく内部においても微生物の培
養が効果的になされ得る。培地成分は発泡体マトリック
ス内に担持されており、培養により得られた生産物中に
混入することは少ない。Since the medium components are contained within the foam matrix, microorganisms can be effectively cultured not only on the surface of the foam but also inside the foam. The medium components are supported within the foam matrix and are less likely to be mixed into the product obtained by culturing.
そのために、生産物の分離が容易になされる。Therefore, separation of the products is facilitated.
この発泡体を用いて静置培養すれば、培地成分の流出が
少なく、液内培養では、培地中の固形分や油脂が発泡体
に吸着されるおそれがないため。If this foam is used for stationary culture, there will be less outflow of medium components, and in submerged culture, there is no risk of solids or fats and oils in the medium being adsorbed by the foam.
微生物の培養効率が高くなる。その結果２本発明の微生
物培養用発泡体は９種々の微生物の培養に有効に利用さ
れ得る。The culture efficiency of microorganisms increases. As a result, the foam for culturing microorganisms of the present invention can be effectively used for culturing various microorganisms.
以上that's all
Claims (1)
る、発泡体マトリックス内に培地成分を含有する微生物
培養用発泡体。 ２、前記発泡体マトリックスが、ポリウレタンフォーム
、ポリスチレン発泡体、塩化ビニル発泡体、ポリエチレ
ン発泡体およびポリエステル発泡体のうちの少なくとも
一種である微生物培養用発泡体。 ３、前記発泡体マトリックス内に、保水力を上げるべく
親水性ポリマーが含有された微生物培養用発泡体。[Scope of Claims] 1. A foam for culturing microorganisms containing a medium component within a foam matrix, which is obtained by foaming a foam composition together with a medium component. 2. A foam for culturing microorganisms, wherein the foam matrix is at least one of polyurethane foam, polystyrene foam, vinyl chloride foam, polyethylene foam, and polyester foam. 3. A foam for culturing microorganisms, in which a hydrophilic polymer is contained in the foam matrix to increase water retention capacity.
Priority Applications (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61222480A JPS6374479A (en) | 1986-09-19 | 1986-09-19 | Foam for cultivation of microorganism |
| FR8712983A FR2604059B1 (en) | 1986-09-19 | 1987-09-18 | PEST EXTERMINATION ELEMENT AND METHOD USING SUCH AN ELEMENT. |
| AU78661/87A AU597424C (en) | 1986-09-19 | 1987-09-18 | Vermin exterminating element and vermin exterminating method using it |
| GB8722035A GB2196645B (en) | 1986-09-19 | 1987-09-18 | Microbiological pesticidal element and method |
| US07/099,262 US4921703A (en) | 1986-09-19 | 1987-09-21 | Vermin exterminating element and vermin exterminating method using it |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61222480A JPS6374479A (en) | 1986-09-19 | 1986-09-19 | Foam for cultivation of microorganism |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6374479A true JPS6374479A (en) | 1988-04-04 |
| JPH0347835B2 JPH0347835B2 (en) | 1991-07-22 |
Family
ID=16783082
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP61222480A Granted JPS6374479A (en) | 1986-09-19 | 1986-09-19 | Foam for cultivation of microorganism |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6374479A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6423888A (en) * | 1987-07-16 | 1989-01-26 | Etsuko Kakizaki | Culture vessel with micro-cellular wall |
| US5589390A (en) * | 1989-09-11 | 1996-12-31 | Nitto Denko Corporation | Vermin exterminating element and vermin exterminating method |
| KR20190030380A (en) * | 2017-09-14 | 2019-03-22 | 김성민 | Forming Tablet Microorganism Medium and the Process for the preparation thereof |
| WO2020184719A1 (en) | 2019-03-14 | 2020-09-17 | 出光興産株式会社 | Pest control material using entomoparasitic microbe and pest control method using same |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS60214878A (en) * | 1984-04-10 | 1985-10-28 | Rikagaku Kenkyusho | Method for cultivating cell |
-
1986
- 1986-09-19 JP JP61222480A patent/JPS6374479A/en active Granted
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS60214878A (en) * | 1984-04-10 | 1985-10-28 | Rikagaku Kenkyusho | Method for cultivating cell |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6423888A (en) * | 1987-07-16 | 1989-01-26 | Etsuko Kakizaki | Culture vessel with micro-cellular wall |
| US5589390A (en) * | 1989-09-11 | 1996-12-31 | Nitto Denko Corporation | Vermin exterminating element and vermin exterminating method |
| KR20190030380A (en) * | 2017-09-14 | 2019-03-22 | 김성민 | Forming Tablet Microorganism Medium and the Process for the preparation thereof |
| WO2020184719A1 (en) | 2019-03-14 | 2020-09-17 | 出光興産株式会社 | Pest control material using entomoparasitic microbe and pest control method using same |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0347835B2 (en) | 1991-07-22 |
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