JPS63248430A - Emulsifier composition - Google Patents
Emulsifier compositionInfo
- Publication number
- JPS63248430A JPS63248430A JP62083553A JP8355387A JPS63248430A JP S63248430 A JPS63248430 A JP S63248430A JP 62083553 A JP62083553 A JP 62083553A JP 8355387 A JP8355387 A JP 8355387A JP S63248430 A JPS63248430 A JP S63248430A
- Authority
- JP
- Japan
- Prior art keywords
- lecithin
- enzyme
- treated
- monoglyceride
- phospholipid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000003995 emulsifying agent Substances 0.000 title claims abstract description 20
- 239000000203 mixture Substances 0.000 title claims abstract description 19
- 239000000787 lecithin Substances 0.000 claims abstract description 38
- 235000010445 lecithin Nutrition 0.000 claims abstract description 38
- 108090000790 Enzymes Proteins 0.000 claims abstract description 36
- 102000004190 Enzymes Human genes 0.000 claims abstract description 36
- 229940067606 lecithin Drugs 0.000 claims abstract description 36
- IIZPXYDJLKNOIY-JXPKJXOSSA-N 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCC\C=C/C\C=C/C\C=C/C\C=C/CCCCC IIZPXYDJLKNOIY-JXPKJXOSSA-N 0.000 claims abstract description 35
- 150000003904 phospholipids Chemical class 0.000 claims abstract description 26
- LDVVTQMJQSCDMK-UHFFFAOYSA-N 1,3-dihydroxypropan-2-yl formate Chemical compound OCC(CO)OC=O LDVVTQMJQSCDMK-UHFFFAOYSA-N 0.000 claims abstract description 15
- 230000001804 emulsifying effect Effects 0.000 abstract description 8
- 235000013305 food Nutrition 0.000 abstract description 4
- 238000002156 mixing Methods 0.000 abstract description 2
- 238000000746 purification Methods 0.000 abstract description 2
- 150000004671 saturated fatty acids Chemical class 0.000 abstract description 2
- UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 abstract description 2
- 235000011837 pasties Nutrition 0.000 abstract 2
- 229940088598 enzyme Drugs 0.000 description 30
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 24
- 238000006243 chemical reaction Methods 0.000 description 17
- 239000007864 aqueous solution Substances 0.000 description 11
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 10
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 10
- 239000000047 product Substances 0.000 description 10
- 238000003756 stirring Methods 0.000 description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 8
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 6
- 239000003921 oil Substances 0.000 description 6
- 235000019198 oils Nutrition 0.000 description 6
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 5
- 239000002253 acid Substances 0.000 description 5
- 239000001110 calcium chloride Substances 0.000 description 5
- 229910001628 calcium chloride Inorganic materials 0.000 description 5
- 238000010438 heat treatment Methods 0.000 description 5
- JLPULHDHAOZNQI-ZTIMHPMXSA-N 1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCC\C=C/C\C=C/CCCCC JLPULHDHAOZNQI-ZTIMHPMXSA-N 0.000 description 4
- OGBUMNBNEWYMNJ-UHFFFAOYSA-N batilol Chemical class CCCCCCCCCCCCCCCCCCOCC(O)CO OGBUMNBNEWYMNJ-UHFFFAOYSA-N 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 229940083466 soybean lecithin Drugs 0.000 description 4
- 102000004882 Lipase Human genes 0.000 description 3
- 108090001060 Lipase Proteins 0.000 description 3
- 239000004367 Lipase Substances 0.000 description 3
- 238000004945 emulsification Methods 0.000 description 3
- 239000000839 emulsion Substances 0.000 description 3
- 239000003925 fat Substances 0.000 description 3
- 238000005194 fractionation Methods 0.000 description 3
- 235000019421 lipase Nutrition 0.000 description 3
- 150000002978 peroxides Chemical class 0.000 description 3
- 150000003626 triacylglycerols Chemical class 0.000 description 3
- ZIIUUSVHCHPIQD-UHFFFAOYSA-N 2,4,6-trimethyl-N-[3-(trifluoromethyl)phenyl]benzenesulfonamide Chemical compound CC1=CC(C)=CC(C)=C1S(=O)(=O)NC1=CC=CC(C(F)(F)F)=C1 ZIIUUSVHCHPIQD-UHFFFAOYSA-N 0.000 description 2
- 240000002791 Brassica napus Species 0.000 description 2
- 235000004977 Brassica sinapistrum Nutrition 0.000 description 2
- 235000003255 Carthamus tinctorius Nutrition 0.000 description 2
- 244000020518 Carthamus tinctorius Species 0.000 description 2
- 244000068988 Glycine max Species 0.000 description 2
- 235000010469 Glycine max Nutrition 0.000 description 2
- 102000015439 Phospholipases Human genes 0.000 description 2
- 108010064785 Phospholipases Proteins 0.000 description 2
- VBICKXHEKHSIBG-UHFFFAOYSA-N beta-monoglyceryl stearate Natural products CCCCCCCCCCCCCCCCCC(=O)OCC(O)CO VBICKXHEKHSIBG-UHFFFAOYSA-N 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- -1 soybean lecithin Chemical compound 0.000 description 2
- 235000012424 soybean oil Nutrition 0.000 description 2
- 239000003549 soybean oil Substances 0.000 description 2
- 230000002195 synergetic effect Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 235000015112 vegetable and seed oil Nutrition 0.000 description 2
- 239000008158 vegetable oil Substances 0.000 description 2
- JQWAHKMIYCERGA-UHFFFAOYSA-N (2-nonanoyloxy-3-octadeca-9,12-dienoyloxypropoxy)-[2-(trimethylazaniumyl)ethyl]phosphinate Chemical compound CCCCCCCCC(=O)OC(COP([O-])(=O)CC[N+](C)(C)C)COC(=O)CCCCCCCC=CCC=CCCCCC JQWAHKMIYCERGA-UHFFFAOYSA-N 0.000 description 1
- RZRNAYUHWVFMIP-KTKRTIGZSA-N 1-oleoylglycerol Chemical compound CCCCCCCC\C=C/CCCCCCCC(=O)OCC(O)CO RZRNAYUHWVFMIP-KTKRTIGZSA-N 0.000 description 1
- FPIPGXGPPPQFEQ-UHFFFAOYSA-N 13-cis retinol Natural products OCC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-UHFFFAOYSA-N 0.000 description 1
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 1
- 108010019160 Pancreatin Proteins 0.000 description 1
- 235000019485 Safflower oil Nutrition 0.000 description 1
- FPIPGXGPPPQFEQ-BOOMUCAASA-N Vitamin A Natural products OC/C=C(/C)\C=C\C=C(\C)/C=C/C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-BOOMUCAASA-N 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- FPIPGXGPPPQFEQ-OVSJKPMPSA-N all-trans-retinol Chemical compound OC\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-OVSJKPMPSA-N 0.000 description 1
- 229910001424 calcium ion Inorganic materials 0.000 description 1
- 229960001231 choline Drugs 0.000 description 1
- OEYIOHPDSNJKLS-UHFFFAOYSA-N choline Chemical compound C[N+](C)(C)CCO OEYIOHPDSNJKLS-UHFFFAOYSA-N 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 238000005187 foaming Methods 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- RZRNAYUHWVFMIP-UHFFFAOYSA-N monoelaidin Natural products CCCCCCCCC=CCCCCCCCC(=O)OCC(O)CO RZRNAYUHWVFMIP-UHFFFAOYSA-N 0.000 description 1
- 235000014593 oils and fats Nutrition 0.000 description 1
- 229940055695 pancreatin Drugs 0.000 description 1
- 235000015277 pork Nutrition 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 239000012264 purified product Substances 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 238000007670 refining Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 235000005713 safflower oil Nutrition 0.000 description 1
- 239000003813 safflower oil Substances 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 229940005741 sunflower lecithin Drugs 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 1
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000019155 vitamin A Nutrition 0.000 description 1
- 239000011719 vitamin A Substances 0.000 description 1
- 229940045997 vitamin a Drugs 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 230000002747 voluntary effect Effects 0.000 description 1
- 238000009736 wetting Methods 0.000 description 1
Landscapes
- Cosmetics (AREA)
- Emulsifying, Dispersing, Foam-Producing Or Wetting Agents (AREA)
- General Preparation And Processing Of Foods (AREA)
- Medicinal Preparation (AREA)
Abstract
Description
【発明の詳細な説明】
fat産業上の利用分野
本発明は乳化剤組成物に関するものであり、詳しくは酵
素処理したリン脂質およびモノグリセリドを有効成分と
する乳化剤組成物に係る。DETAILED DESCRIPTION OF THE INVENTION Field of Industrial Application of FAT The present invention relates to an emulsifier composition, and more particularly to an emulsifier composition containing enzyme-treated phospholipids and monoglycerides as active ingredients.
(b)従来の技術
レシチンは乳化・起泡の安定化および湿潤化の促進等の
ための乳化剤として広く食品加工分野において使用され
ている。最近、レシチンをより親水化あるいは親油化し
たモディファイド(Modified)レシチンがいく
つか開発され、一層の乳化力の向上がみられている。そ
のうち酵素処理レシチンは特にレシチンの極性が高めら
れ、O/W乳化能が改善されており食品加工分野などに
おいて有用である。(b) Prior Art Lecithin is widely used in the food processing field as an emulsifier for stabilizing emulsification/foaming and promoting wetting. Recently, several modified lecithins made by making lecithin more hydrophilic or lipophilic have been developed, and further improvement in emulsifying power has been observed. Among them, enzyme-treated lecithin has particularly increased lecithin polarity and improved O/W emulsification ability, and is useful in the food processing field.
(C)発明が解決しようとする問題点
しかし乍ら、酵素処理レシチンの乳化能をさらに向上さ
せることができれば、一層すぐれた乳化剤となり、より
価値の高い製品が期待できる。(C) Problems to be Solved by the Invention However, if the emulsifying ability of enzyme-treated lecithin can be further improved, it can be expected that it will become an even better emulsifier and a product of higher value.
本発明の目的は酵素処理レシチンに比べ、さらに乳化能
が高い乳化剤組成物を提供することにある。An object of the present invention is to provide an emulsifier composition that has higher emulsifying ability than enzyme-treated lecithin.
(d1問題点を解決するための手段
本発明者らは、鋭意研究の結果、酵素処理したリン脂質
にモノグリセリドを添加することにより、その乳化能が
飛躍的に向上することを見い出した。(Means for solving the d1 problem) As a result of extensive research, the present inventors discovered that by adding monoglyceride to enzyme-treated phospholipids, the emulsifying ability of the phospholipids was dramatically improved.
即ち、本発明は酵素処理したリン脂質とモノグリセリド
とを含有してなる乳化剤組成物である。That is, the present invention is an emulsifier composition containing an enzyme-treated phospholipid and a monoglyceride.
本発明でいうリン脂質とは、商業的にはレシチンと称さ
れているものである。レシチンには植物油の精製過程で
副生される、大豆レシチン、なたねレシチン、サフラワ
ーレシチン、ひまわりレシチン等の植物性レシチンおよ
び卵黄レシチン等が挙げられる。それらのうちで価格、
供給等の面から大豆レシチンが有利である。The phospholipid referred to in the present invention is commercially referred to as lecithin. Examples of lecithin include vegetable lecithin such as soybean lecithin, rapeseed lecithin, safflower lecithin, and sunflower lecithin, which are by-produced during the refining process of vegetable oil, and egg yolk lecithin. Among them the price,
Soybean lecithin is advantageous from the viewpoint of supply.
レシチンにはその精製の度合により、脱ガム油滓、これ
を脱水したトリグリセリドを含有するペースト状レシチ
ン、それをアセトン分別等によりトリグリセリドを除去
した粉末状レシチン、あるいは溶剤やカラム分画により
フォスファチジルコリン含量を高めた精製高純度レシチ
ンがある。本発明においては、これらのいずれのレシチ
ンを用いてもよい。本発明に用いる酵素としては、リン
脂質のエステル結合を特異的に加水分解するフォスフォ
リバーゼA(以下PL−Aという)を挙げることができ
る。Depending on the degree of purification, lecithin can be made from degummed soapstock, paste lecithin containing triglycerides obtained by dehydrating it, powdered lecithin obtained by removing triglycerides by acetone fractionation, or phosphatidyl lecithin obtained by solvent or column fractionation. There is purified high-purity lecithin with increased choline content. In the present invention, any of these lecithins may be used. Examples of the enzyme used in the present invention include phospholibase A (hereinafter referred to as PL-A), which specifically hydrolyzes ester bonds in phospholipids.
酵素処理したリン脂質は本発明の乳化剤中1%(重量。The enzyme-treated phospholipid is 1% (by weight) in the emulsifier of the present invention.
以下同様)以上含有することが望ましい。The same applies hereafter) or more is desirable.
また、リン脂質は必ずしも全てが酵素処理を受けている
必要はないが、酵素処理リン脂質が全リン脂質の10%
以上含有されていることが好ましい。Furthermore, although not all phospholipids need to be enzyme-treated, enzyme-treated phospholipids make up 10% of all phospholipids.
It is preferable that the content is above.
酵素処理したリン脂質の量が上記に満たないときは、実
質的に効果があられれない。When the amount of enzyme-treated phospholipid is less than the above amount, there is no substantial effect.
本発明に用いる1モノグリセリドとしては、その乳化安
定性の面から飽和脂肪酸を含むモノグリセリドが望まし
く、例えば、ステアリン酸モノグリセリドを用いること
ができる。また、目的に応じ、不飽和脂肪酸のモノグリ
セリドを用いてもよい。The monoglyceride used in the present invention is preferably a monoglyceride containing a saturated fatty acid from the viewpoint of emulsion stability, and for example, stearic acid monoglyceride can be used. Furthermore, monoglycerides of unsaturated fatty acids may be used depending on the purpose.
モノグリセリドは粗モノグリセリドであっても精製品で
あってもまた、ジグリセリドあるいはトリグリセリドを
含むものであっても差し支えない。The monoglyceride may be a crude monoglyceride, a purified product, or one containing diglyceride or triglyceride.
モノグリセリドの量は酵素処理リン脂質量の1%以上好
ましくは10%程度である。1%に満たないときは、モ
ノグリセリドによる相乗効果が実質上みられない。The amount of monoglyceride is 1% or more, preferably about 10% of the amount of enzyme-treated phospholipid. When the amount is less than 1%, there is virtually no synergistic effect due to monoglyceride.
本発明の酵素処理リン脂質は例えば次のようにして製造
される。即ち、リン脂質を水に分散させ乳化液とする。The enzyme-treated phospholipid of the present invention is produced, for example, as follows. That is, phospholipids are dispersed in water to form an emulsion.
水酸化ナトリウム等のアルカリを用いてPL−Aの至適
pHであるアルカリ側、好ましくはpH8〜10に調整
する。さらにPL−Aがカルシウムイオン要求性の酵素
であるため、1mM程度の塩化カルシウムを添加する。Using an alkali such as sodium hydroxide, the optimum pH of PL-A is adjusted to the alkaline side, preferably pH 8 to 10. Furthermore, since PL-A is an enzyme that requires calcium ions, approximately 1 mM calcium chloride is added.
酵素の使用量としてはリン脂質に対して重量比で1/1
00〜1150,000がよ(、さらに好ましくは1/
1.000〜1/10.000である。反応温度は酵素
の至適温度にするのが好ましいが、レシチンの熱による
劣化を防止するために50℃を上限とすることが望まし
い。The amount of enzyme used is 1/1 in weight ratio to phospholipid.
00 to 1,150,000 (more preferably 1/
It is 1.000 to 1/10.000. Although the reaction temperature is preferably set to the optimum temperature for the enzyme, it is desirable to set the upper limit to 50° C. in order to prevent deterioration of lecithin due to heat.
酵素反応はうなり広範囲のpHで進行するが、反応に伴
って、精製する脂肪酸によるpHの添加を防ぐため、適
当量の水酸化ナトリウム等のアルカリ水溶液を加え至適
pHを維持する。反応時間は使用する酵素量、pH1温
度等によって異なるがおおよそ2〜8時間でよい。反応
の停止は適当量の塩酸等の酸を加え、pHを中性にする
ことで達成される。その後乾燥して酵素処理リン脂質を
得る。The enzymatic reaction proceeds over a wide range of pH, but in order to prevent the fatty acid to be purified from adding to the pH during the reaction, an appropriate amount of an alkaline aqueous solution such as sodium hydroxide is added to maintain the optimum pH. The reaction time varies depending on the amount of enzyme used, pH, temperature, etc., but may be approximately 2 to 8 hours. The reaction is stopped by adding an appropriate amount of an acid such as hydrochloric acid to neutralize the pH. Thereafter, it is dried to obtain enzyme-treated phospholipids.
(e)実施例
実施例1
ペースト状レシチン(日清製油■製 レシチンDX)4
0gを温水160gに分散させ、ホモミキサーで乳化す
る。IN水酸化ナトリウム水溶液を適量加え、pHを8
.0に調整し、1M塩化カルシウム水溶液を0.2mj
!添加する。50℃に加温後PL−A (ノボインダス
トリー社製 レシターゼ10 L) 0.02mj2添
加し、攪拌しつつ反応を行う。反応中、適量のNaOH
水溶液を添加しつつ、pHを8.0〜9.0に維持する
。6時間後適当量の塩酸を加えpHを7.0に調整し、
反応を停止する。濃縮・乾燥を行い18gの酵素処理レ
シチンを得る。(e) Examples Example 1 Paste lecithin (Lecithin DX manufactured by Nisshin Oil Co., Ltd.) 4
Disperse 0g in 160g of warm water and emulsify with a homomixer. Add appropriate amount of IN sodium hydroxide solution to adjust pH to 8.
.. 0, and add 0.2 mj of 1M calcium chloride aqueous solution.
! Added. After heating to 50° C., 0.02 mj2 of PL-A (Lecitase 10 L manufactured by Novo Industries) was added, and the reaction was carried out with stirring. During the reaction, an appropriate amount of NaOH
Maintain the pH between 8.0 and 9.0 while adding the aqueous solution. After 6 hours, add an appropriate amount of hydrochloric acid to adjust the pH to 7.0.
Stop the reaction. Concentration and drying are performed to obtain 18 g of enzyme-treated lecithin.
オレイン酸モノグリセリド(理研ビタミン■製エマルジ
ーMO)0.18 gを添加し、充分混合し製品を得る
。Add 0.18 g of oleic acid monoglyceride (Emulgy MO manufactured by Riken Vitamin ■) and mix thoroughly to obtain a product.
このものは酸価は65、過酸化物価01アセトン不溶分
55%である。This product has an acid value of 65, a peroxide value of 0, and an acetone insoluble content of 55%.
実施例2
ペースト状レシチンから?容剤を用いてトリグリセリド
を除去した粉末状大豆レシチン(日清製油■製 PC−
30)20gを温水180gに分散させ、ホモミキサー
で均一に懸濁する。IN水酸化カリウム水溶液を適当量
加えpHを8.0に調整し、1M塩化カルシウム水溶液
を0.2mj!添加する。Example 2 From paste lecithin? Powdered soybean lecithin from which triglycerides have been removed using a container (PC- manufactured by Nisshin Oil Co., Ltd.)
30) Disperse 20g in 180g of warm water and suspend uniformly with a homomixer. Add an appropriate amount of IN potassium hydroxide aqueous solution to adjust the pH to 8.0, and add 0.2 mj! of 1M calcium chloride aqueous solution! Added.
50℃に加温後PL−Aの酵素源として豚パンクレアチ
ン(和光純薬工業■M)を0.2g添加し、攪拌しつつ
反応を行う。反応中、適量のIN水酸化カリウム水溶液
を加えつつpHを8.0〜9.0に維持する。8時間後
に塩酸を加えpHを760にし反応を終了する。After heating to 50° C., 0.2 g of pork pancreatin (Wako Pure Chemical Industries, Ltd. M) was added as an enzyme source for PL-A, and the reaction was carried out with stirring. During the reaction, the pH is maintained at 8.0 to 9.0 while adding an appropriate amount of IN potassium hydroxide aqueous solution. After 8 hours, hydrochloric acid was added to adjust the pH to 760 to complete the reaction.
ステアリン酸モノグリセリド(理研ビタミンa菊製 エ
マルジーMS)0.2gを添加し充分に攪拌混合した後
、濃縮・乾燥して製品を得る。After adding 0.2 g of stearic acid monoglyceride (Emulgy MS manufactured by Riken Vitamin A Kiku) and thoroughly stirring and mixing, the mixture is concentrated and dried to obtain a product.
このものは酸価は66、過酸化物価0、アセトン不溶分
97%である。This product has an acid value of 66, a peroxide value of 0, and an acetone insoluble content of 97%.
実施例3
溶剤およびカラム分画処理により得た精製高純度大豆レ
シチン(日清製油■製 PC−70)20gを温水18
0gに分散させ、ホモミキサーで均一に懸濁する。IN
水酸化ナトリウム水溶液を適当量加えpHを8.0に調
整し、1M塩化カルシウム水溶液を0.2mJ添加する
。50℃に加温後PL−A (シグマ社製 フォスフォ
リパーゼAZ)を50■添加し、攪拌しつつ反応を行う
。反応中通量のIN水酸化ナトリウム水溶液を加えつつ
、pHを8.0〜9.0に維持する。4時間後に塩酸を
加え、pHを7.0にし反応を停止する。Example 3 20 g of purified high-purity soybean lecithin (PC-70 manufactured by Nisshin Oil Co., Ltd.) obtained by solvent and column fractionation treatment was added to 18 g of warm water.
0g and homogeneously suspended using a homomixer. IN
Add an appropriate amount of sodium hydroxide aqueous solution to adjust the pH to 8.0, and add 0.2 mJ of 1M calcium chloride aqueous solution. After heating to 50°C, 50 μ of PL-A (phospholipase AZ, manufactured by Sigma) was added, and the reaction was carried out with stirring. The pH is maintained between 8.0 and 9.0 while adding the same amount of IN sodium hydroxide solution during the reaction. After 4 hours, hydrochloric acid is added to adjust the pH to 7.0 to stop the reaction.
サフラワー油モノグリセリド(太陽化学■製すンソフト
m8090) 0.2gを添加し、充分に攪拌混合し、
濃縮・乾燥後19gの製品を得る。Add 0.2 g of safflower oil monoglyceride (Taiyo Kagaku Seisun Soft M8090) and stir and mix thoroughly.
After concentration and drying, 19 g of product is obtained.
このものは酸価71、過酸化物価0、アセトン不溶分9
8%である。This product has an acid value of 71, a peroxide value of 0, and an acetone insoluble content of 9.
It is 8%.
実験例
実施例1〜3において製造された乳化剤組成物の乳化テ
ストを行ったところ、第1表に示す結果が得られた。Experimental Examples When the emulsifier compositions prepared in Examples 1 to 3 were subjected to emulsification tests, the results shown in Table 1 were obtained.
乳化力のテストは以下のとおりに行った。乳化剤組成物
0.2gを添加した大豆白絞油50gを60℃に加温す
る。60℃に加温した水をホモミキサーで600Orp
mで攪拌しつつ、乳化剤を添加した大豆白絞油を徐々に
滴下し、6000rpmで5分間乳化する。乳化液30
ml1をメスシリンダーにとり、室温で静置する。6時
間後に乳化層の割合を測定する。The emulsifying power test was conducted as follows. 50 g of white soybean oil to which 0.2 g of the emulsifier composition was added is heated to 60°C. Water heated to 60℃ was heated to 600Orp using a homomixer.
While stirring at m, the soybean white squeezed oil containing an emulsifier was gradually added dropwise and emulsified at 6000 rpm for 5 minutes. emulsion 30
Transfer 1 ml to a graduated cylinder and let stand at room temperature. After 6 hours, the proportion of the emulsified layer is measured.
対照として本発明によらない各種の試料について同様に
乳化力を測定した。As a control, the emulsifying power of various samples not according to the present invention was similarly measured.
第1表
試 料 乳化層
の割合
酵素処理レシチンDX十エマルジーMO(実31例1)
981酵素処理PC−30+工マルジーMS (
実施例2) 1001酵素処理PC−70+サ
ンソフト魔8090 (実施例3) 100酵素処
理レシチンDX 54酵
素処理PC−3071
酵素処理PC−7073
レシチンDX+工マルジーM0 62
PC−30+工マルジーM3 6
BPC−70+サンソフト階8090
70レシチンDX
62PC−3066
DC−7867
(f)発明の効果
本発明によれば、酵素処理したリン脂質とモノグリセリ
ドの相乗効果により、乳化力の高い乳化剤組成物を得る
ことができる。この効果は酵素処理したリン脂質のみや
、酵素処理しないリン脂質とモノグリセリドとの組み合
わせからは予測できないものである。Table 1 Sample Emulsified layer ratio Enzyme-treated lecithin DX 10 Emulgy MO (Example 31 1)
981 Enzyme treatment PC-30 + Engineering Margy MS (
Example 2) 1001 Enzyme-treated PC-70 + Sunsoft Ma 8090 (Example 3) 100 Enzyme-treated Lecithin DX 54 Enzyme-treated PC-3071 Enzyme-treated PC-7073 Lecithin DX + Enzyme-treated Lecithin M0 62
PC-30 + Engineering Margy M3 6
BPC-70+Sunsoft floor 8090
70 lecithin DX
62PC-3066 DC-7867 (f) Effects of the Invention According to the present invention, an emulsifier composition with high emulsifying power can be obtained due to the synergistic effect of enzyme-treated phospholipids and monoglycerides. This effect cannot be predicted from enzyme-treated phospholipids alone or from a combination of non-enzyme-treated phospholipids and monoglycerides.
本発明の乳化剤は特に食品用として最適であり、さらに
医薬品、化粧品、その他の用途でのすぐれた効果を特徴
する
特許出願人 日清製油株式会社
手続補正書(自発)
1.事件の表示
昭和62特許許願第83553号
2、発明の名称
乳化剤組成物
3、補正をする者
事件との関係 特許出願人
住 所 東京都中央区新川−丁目23番1号本件に
関する連絡は下記にお願いします。The emulsifier of the present invention is especially suitable for food use, and is characterized by excellent effects in pharmaceuticals, cosmetics, and other uses.Patent Applicant: Nisshin Oil Co., Ltd. Procedural Amendment (Voluntary) 1. Description of the case 1988 Patent Application No. 83553 2 Name of the invention Emulsifier composition 3 Person making the amendment Relationship to the case Patent applicant Address 23-1 Shinkawa-chome, Chuo-ku, Tokyo For correspondence regarding this case, please contact the following: please.
郵便番号 221
住 所 神奈川県横浜市神奈用区千若町1−3名
称 日清製油株式会社 研究所型 話 0
45 (461)01204、補正の対象
明細書の発明の詳細な説明の欄
5、補正の内容
(1)明細書第4頁11行の次に以下の文章を挿入する
。Postal code 221 Address 1-3 Chiwaka-cho, Kanayō-ku, Yokohama-shi, Kanagawa Prefecture
Name Nisshin Oil Co., Ltd. Laboratory type Story 0
45 (461)01204, Column 5 of Detailed Description of the Invention of the Specification Subject to Amendment, Contents of Amendment (1) The following sentence is inserted next to line 11 on page 4 of the specification.
「そして、これらは市販の合成品でもよいし、また、例
えば油脂を加水分解して用いることもできる。後者の場
合を例示すれば、油脂に1710〜1倍量の水を加え懸
濁した後、重量比で1/100〜1/100.000.
好ましくは115.000〜1/10,000量のリパ
ーゼを添加し、50℃で0.5〜2時間反応させ、部分
的にエステル結合を加水分解する。用いる油脂としては
、大豆白絞油、なたね白絞油、サフラワー白絞油等の植
物油が好ましい。」
(2)同第8頁10行の次に以下の文章を挿入する。``These may be commercially available synthetic products, or they may be used, for example, by hydrolyzing fats and oils.In the latter case, for example, after adding 1710 to 1 times the amount of water to fats and oils and suspending them, , weight ratio 1/100 to 1/100.000.
Preferably, 115,000 to 1/10,000 of the amount of lipase is added and reacted at 50° C. for 0.5 to 2 hours to partially hydrolyze the ester bonds. The oils and fats used are preferably vegetable oils such as soybean white squeezed oil, rapeseed white squeezed oil, and safflower white squeezed oil. (2) Insert the following sentence after line 10 on page 8.
「実施例4
実施例2で用いたPC−3020gを温水180gに分
散し、ホモミキサーで均一に懸濁する。"Example 4 20 g of PC-30 used in Example 2 is dispersed in 180 g of warm water and suspended uniformly using a homomixer.
IN水酸化ナトリウム水溶液を適量加え、pHを8.0
に調整し、1M塩化カルシウム水溶液を0.2mg添加
する。Add appropriate amount of IN sodium hydroxide aqueous solution to adjust pH to 8.0.
and add 0.2 mg of 1M calcium chloride aqueous solution.
50℃に加温後、フォスフォリパーゼAt (シグマ
社製)を4mg添加し、撹拌しつつ反応を行う。After heating to 50°C, 4 mg of phospholipase At (manufactured by Sigma) is added, and the reaction is carried out with stirring.
反応中通量の水酸化ナトリウム水溶液を加えつつ、pH
を8.0〜9.0に維持する。4時間後、適量の塩酸を
加え、pHを7.0とし反応を停止する。別に大豆白絞
油2gに水0.2g添加し、ホモジナイザーで充分懸濁
する。50℃に加温後リパーゼ(名糖産業製 リパーゼ
OF)を1mg添加し、攪拌しつつ2時間反応を行う。While adding the same amount of sodium hydroxide aqueous solution during the reaction, adjust the pH.
Maintain between 8.0 and 9.0. After 4 hours, add an appropriate amount of hydrochloric acid to adjust the pH to 7.0 and stop the reaction. Separately, add 0.2 g of water to 2 g of white soybean oil and suspend thoroughly using a homogenizer. After heating to 50°C, 1 mg of lipase (Lipase OF, manufactured by Meito Sangyo) is added, and reaction is carried out for 2 hours while stirring.
反応後前者と混合し、濃縮・乾燥し製品20gを得る。After the reaction, it is mixed with the former, concentrated and dried to obtain 20 g of product.
このものは酸価75、アセトン不溶分50%である。」
(3)同第8頁12行「実施例1〜3」を「実施例1〜
4」と訂正する。This product has an acid value of 75 and an acetone insoluble content of 50%. ”
(3) Change “Examples 1 to 3” to “Examples 1 to 3” on page 8, line 12.
4” is corrected.
(4)同第9頁第1表を次のように訂正する。(4) Table 1 on page 9 is corrected as follows.
「 第1表
手続補正書 4゜
昭和6年10川5日
ζ
2、発明の名称
乳化剤組成物
3、補正をする者
事件との関係 特許出願人
住所 東京都中央区新川−丁目23番1号本件に関す
る連絡は下記にお願いします。" Table 1 Procedural Amendment 4゜ 10 Kawa 5, 1930 ζ 2. Name of the invention Emulsifier composition 3. Relationship to the person making the amendment Patent applicant's address 23-1 Shinkawa-chome, Chuo-ku, Tokyo Please contact us below regarding this matter.
郵便番号 221
住 所 神奈川県横浜市神奈用区千若町1−3補正の
対象
(11明細書の発明の詳細な説明の欄
補正の内容
(11明細書第5頁8行「うなり」を「かなり」と訂正
する。Postal code 221 Address 1-3 Chiwaka-cho, Kanayō-ku, Yokohama-shi, Kanagawa Prefecture Target of amendment (11 Contents of amendment in the column of detailed description of the invention in the specification (11 Change ``beat'' to page 5, line 8 of the specification) Quite a bit,” he corrected.
(2)同第5頁9行「精製」を「生成」と訂正する。(2) On page 5, line 9, ``purification'' is corrected to ``production.''
(3)同第5頁9行「添加」を「低下」と訂正する。(3) On page 5, line 9, "addition" is corrected to "reduction."
Claims (2)
有してなる乳化剤組成物。(1) An emulsifier composition containing an enzyme-treated phospholipid and a monoglyceride.
、粉末状レシチンまたは精製高純度レシチンを用いる特
許請求の範囲第1項記載の乳化剤組成物。(2) The emulsifier composition according to claim 1, which uses degummed soapstock, paste lecithin, powdered lecithin, or purified high-purity lecithin as the phospholipid.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP62083553A JPS63248430A (en) | 1987-04-04 | 1987-04-04 | Emulsifier composition |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP62083553A JPS63248430A (en) | 1987-04-04 | 1987-04-04 | Emulsifier composition |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS63248430A true JPS63248430A (en) | 1988-10-14 |
JPH0512977B2 JPH0512977B2 (en) | 1993-02-19 |
Family
ID=13805701
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP62083553A Granted JPS63248430A (en) | 1987-04-04 | 1987-04-04 | Emulsifier composition |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS63248430A (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1997028270A1 (en) * | 1996-02-02 | 1997-08-07 | Biomolecular Products, Inc. | Methods for making lysophosphatidylcholine |
US5891466A (en) * | 1990-08-13 | 1999-04-06 | Yesair; David W. | Mixed Liped-Bicarbonate colloidal particles for delivering drugs or calories |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS59122423A (en) * | 1982-12-28 | 1984-07-14 | Green Cross Corp:The | Carcinostatic-containing fatty emulsion |
JPS61171407A (en) * | 1985-01-23 | 1986-08-02 | Kanebo Ltd | Emulsion type cosmetic |
JPS61186305A (en) * | 1985-02-14 | 1986-08-20 | Kanebo Ltd | Emulsion type cosmetic |
JPS6265646A (en) * | 1985-09-19 | 1987-03-24 | Kyowa Hakko Kogyo Co Ltd | Food quality improver and improvement of food quality using said improver |
JPS6354926A (en) * | 1986-06-18 | 1988-03-09 | Asahi Denka Kogyo Kk | Surfactant composition |
-
1987
- 1987-04-04 JP JP62083553A patent/JPS63248430A/en active Granted
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS59122423A (en) * | 1982-12-28 | 1984-07-14 | Green Cross Corp:The | Carcinostatic-containing fatty emulsion |
JPS61171407A (en) * | 1985-01-23 | 1986-08-02 | Kanebo Ltd | Emulsion type cosmetic |
JPS61186305A (en) * | 1985-02-14 | 1986-08-20 | Kanebo Ltd | Emulsion type cosmetic |
JPS6265646A (en) * | 1985-09-19 | 1987-03-24 | Kyowa Hakko Kogyo Co Ltd | Food quality improver and improvement of food quality using said improver |
JPS6354926A (en) * | 1986-06-18 | 1988-03-09 | Asahi Denka Kogyo Kk | Surfactant composition |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5891466A (en) * | 1990-08-13 | 1999-04-06 | Yesair; David W. | Mixed Liped-Bicarbonate colloidal particles for delivering drugs or calories |
WO1997028270A1 (en) * | 1996-02-02 | 1997-08-07 | Biomolecular Products, Inc. | Methods for making lysophosphatidylcholine |
Also Published As
Publication number | Publication date |
---|---|
JPH0512977B2 (en) | 1993-02-19 |
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