JPS6317901A - Purification of chitosan - Google Patents
Purification of chitosanInfo
- Publication number
- JPS6317901A JPS6317901A JP15979386A JP15979386A JPS6317901A JP S6317901 A JPS6317901 A JP S6317901A JP 15979386 A JP15979386 A JP 15979386A JP 15979386 A JP15979386 A JP 15979386A JP S6317901 A JPS6317901 A JP S6317901A
- Authority
- JP
- Japan
- Prior art keywords
- chitosan
- precipitate
- acid
- containing liquid
- adjusted
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 229920001661 Chitosan Polymers 0.000 title claims abstract description 57
- 238000000746 purification Methods 0.000 title description 12
- 239000002244 precipitate Substances 0.000 claims abstract description 36
- 238000000034 method Methods 0.000 claims abstract description 16
- 239000002253 acid Substances 0.000 claims abstract description 14
- 239000007788 liquid Substances 0.000 claims abstract description 14
- 238000005406 washing Methods 0.000 claims abstract description 6
- 238000004090 dissolution Methods 0.000 claims abstract description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 abstract description 16
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 abstract description 15
- 239000012535 impurity Substances 0.000 abstract description 8
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 abstract description 4
- 239000003513 alkali Substances 0.000 abstract description 4
- 238000001556 precipitation Methods 0.000 abstract description 3
- 230000006196 deacetylation Effects 0.000 abstract description 2
- 238000003381 deacetylation reaction Methods 0.000 abstract description 2
- 239000006185 dispersion Substances 0.000 abstract description 2
- 239000003814 drug Substances 0.000 abstract description 2
- 230000001376 precipitating effect Effects 0.000 abstract 1
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 15
- MSWZFWKMSRAUBD-IVMDWMLBSA-N 2-amino-2-deoxy-D-glucopyranose Chemical compound N[C@H]1C(O)O[C@H](CO)[C@@H](O)[C@@H]1O MSWZFWKMSRAUBD-IVMDWMLBSA-N 0.000 description 6
- 229920002101 Chitin Polymers 0.000 description 6
- MSWZFWKMSRAUBD-UHFFFAOYSA-N beta-D-galactosamine Natural products NC1C(O)OC(CO)C(O)C1O MSWZFWKMSRAUBD-UHFFFAOYSA-N 0.000 description 6
- 229960002442 glucosamine Drugs 0.000 description 6
- 238000005119 centrifugation Methods 0.000 description 5
- 235000011121 sodium hydroxide Nutrition 0.000 description 5
- 241000238557 Decapoda Species 0.000 description 4
- 239000004744 fabric Substances 0.000 description 4
- 235000019645 odor Nutrition 0.000 description 4
- 239000003086 colorant Substances 0.000 description 3
- 239000002195 soluble material Substances 0.000 description 3
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 239000003205 fragrance Substances 0.000 description 2
- 230000007062 hydrolysis Effects 0.000 description 2
- 238000006460 hydrolysis reaction Methods 0.000 description 2
- 230000003301 hydrolyzing effect Effects 0.000 description 2
- 230000009965 odorless effect Effects 0.000 description 2
- 239000000049 pigment Substances 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 230000001953 sensory effect Effects 0.000 description 2
- 241000131500 Chionoecetes opilio Species 0.000 description 1
- 235000008733 Citrus aurantifolia Nutrition 0.000 description 1
- 241000238424 Crustacea Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- DUKURNFHYQXCJG-UHFFFAOYSA-N Lewis A pentasaccharide Natural products OC1C(O)C(O)C(C)OC1OC1C(OC2C(C(O)C(O)C(CO)O2)O)C(NC(C)=O)C(OC2C(C(OC3C(OC(O)C(O)C3O)CO)OC(CO)C2O)O)OC1CO DUKURNFHYQXCJG-UHFFFAOYSA-N 0.000 description 1
- 241000237852 Mollusca Species 0.000 description 1
- OVRNDRQMDRJTHS-FMDGEEDCSA-N N-acetyl-beta-D-glucosamine Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O OVRNDRQMDRJTHS-FMDGEEDCSA-N 0.000 description 1
- 235000011941 Tilia x europaea Nutrition 0.000 description 1
- 241000251539 Vertebrata <Metazoa> Species 0.000 description 1
- 230000004931 aggregating effect Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 239000012043 crude product Substances 0.000 description 1
- 230000000850 deacetylating effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000002523 gelfiltration Methods 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 239000002198 insoluble material Substances 0.000 description 1
- 239000004571 lime Substances 0.000 description 1
- 150000007522 mineralic acids Chemical class 0.000 description 1
- 229950006780 n-acetylglucosamine Drugs 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 238000010979 pH adjustment Methods 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 239000012264 purified product Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 239000001054 red pigment Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Landscapes
- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
Description
【発明の詳細な説明】 本発明はキトサンの精製法に関するものである。[Detailed description of the invention] The present invention relates to a method for purifying chitosan.
一般に、キトサン（ｃｈｉｔｏｓａｎ）はカニの甲羅な
どの主成分であるキチンを脱アセチルして得られる塩基
性多糖である。キトサンの原料となるキチンは無を椎動
物、特にエビやカニ等の水産の甲殻類をはじめ、陸産の
昆虫類の外殻成分、さらに、軟体動物の外皮成分、菌類
の細胞壁中などに広く存在しており、その年間の生合成
量は、数十億トンとみられているものの、殆ど利用され
てない資源のひとつである。最も利用されている、エビ
、カニの外殻成分は、このキチンとタンパク質及び、炭
酸カルシウムから成る複合素材で、キチンにする為には
、希アルカリでタンパク質を、希酸で石灰分を除去しな
くてはならない。このようにして得られたキチンはＮ−
アセチルグルコサミンが直列にβ−１，４結合した分子
量数十万の高分子物質である。このキチンを４０〜５０
％の濃アルカリ存在下で加熱して、脱アセチル化すると
、キトサンが得られる。しかし、その性状は未だ甲羅の
名残を留め、カニ殻臭がかなり強く感じられ、かつ甲羅
由来の淡赤色の色素も残存しているものである。Generally, chitosan is a basic polysaccharide obtained by deacetylating chitin, which is a main component of crab shells and the like. Chitin, the raw material for chitosan, is widely found in vertebrates, especially marine crustaceans such as shrimp and crabs, the outer shell components of terrestrial insects, the outer shell components of molluscs, and the cell walls of fungi. Although the annual biosynthesis amount is estimated to be several billion tons, it is one of the least utilized resources. The outer shell components of shrimp and crabs, which are most used, are a composite material consisting of chitin, protein, and calcium carbonate.To make chitin, the protein is removed with dilute alkali and the lime content is removed with dilute acid. Must-have. The chitin thus obtained is N-
It is a polymer substance with a molecular weight of several hundred thousand, consisting of acetylglucosamine linked in series with β-1,4 bonds. This chitin is 40-50
Deacetylation by heating in the presence of % concentrated alkali yields chitosan. However, it still retains the remains of its shell, has a very strong crab shell odor, and also retains the pale red pigment derived from its shell.
従来、キトサンは、その強い凝集力を利用して、各種分
散物の凝集剤として広く使用されて来た。Conventionally, chitosan has been widely used as a flocculant for various dispersions due to its strong cohesive force.
また、近年になって、キトサンに薬理効果が期待され、
医薬としての利用が研究されるようになって来た。その
為、不純物の存在、精製度の程度が問題となり、場合に
よっては、その有用性をも左右することができる。In addition, in recent years, chitosan has been expected to have pharmacological effects.
Research has begun on its use as a medicine. Therefore, the presence of impurities and the degree of purification become a problem, and in some cases, their usefulness can be affected.
しかしながら、キトサン自体凝集作用が非常に強く、精
製が困難で、純品を大量に得ることはできなかった。However, chitosan itself has a very strong aggregating effect, making purification difficult and making it impossible to obtain a pure product in large quantities.
本発明者らは、キトサンを工業的に大量に精製する方法
を求めて鋭意研究したところ、キトサン溶液に、各種の
狭雑物があっても、ｐＨ６，０以上へのｐＨ調整だけで
キトサンだけの析出が起ることを知ったのである。The present inventors conducted extensive research in search of a method for industrially purifying chitosan in large quantities, and found that even if there were various impurities in the chitosan solution, chitosan could be purified by simply adjusting the pH to 6.0 or higher. I learned that precipitation occurs.
また、本発明においては、ｐＨ６，０以上のｐＨ調整に
よるキトサン析出物は、分離して酸に良好に溶解するの
で、再びキトサン溶液にして、ｐＨ６，０以上へのｐＨ
調整によりキトサンを析出させれば、著じるしく精製さ
れたキトサンを得ることができるものである。In addition, in the present invention, chitosan precipitates resulting from pH adjustment to pH 6.0 or higher are separated and dissolved well in acid, so they are made into a chitosan solution again and adjusted to pH 6.0 or higher.
If chitosan is precipitated through adjustment, significantly purified chitosan can be obtained.
本発明は、キトサン含有液をｐＨ６，０以上、好ましく
はｐＨ６，５以上に調整し、析出物を生成せしめること
を特徴とするキトサンの精製法である。The present invention is a chitosan purification method characterized by adjusting a chitosan-containing liquid to pH 6.0 or higher, preferably pH 6.5 or higher, and generating a precipitate.
また、本発明は、キトサン含有液をｐＨ６．０以上、好
ましくはｐＨ６．５に調整し、得られた析出物を必要に
応じて洗滌し、該析出物を酸に溶解することを特徴とす
るキトサンの精製法である。Further, the present invention is characterized in that the chitosan-containing liquid is adjusted to pH 6.0 or higher, preferably pH 6.5, the obtained precipitate is washed as necessary, and the precipitate is dissolved in an acid. This is a method for purifying chitosan.
また、本発明は、キトサン含有液をｐＨ６，０以上、好
ましくはｐＨ６，５に調整し、得られた析出物を必要に
応じて水洗し、該析出物を酸に溶解し、ｐＨ６以上、好
ましくはｐＨ６．５以上に調整し、得られた析出物を必
要に応じて洗滌し、再び析出物を酸に溶解し、必要があ
れば上記の析出−洗滌−溶解の操作をくり返すことを特
徴とするキトサンの精製法である。In addition, the present invention adjusts the chitosan-containing liquid to pH 6.0 or higher, preferably pH 6.5, washes the obtained precipitate with water as needed, dissolves the precipitate in acid, and adjusts the chitosan-containing liquid to pH 6.0 or higher, preferably pH 6.5. is characterized by adjusting the pH to 6.5 or higher, washing the obtained precipitate as necessary, dissolving the precipitate in acid again, and repeating the above precipitation-washing-dissolution operation if necessary. This is a method for purifying chitosan.
本発明の精製法に適用されるキトサン含有液としては、
夾雑物の多い脱アセチル化処理した処理液などいずれで
もよい。The chitosan-containing liquid applicable to the purification method of the present invention includes:
Any treatment solution, such as a deacetylated treatment solution containing many impurities, may be used.
キトサン含有液をｐＨ６以上、好ましくは、ｐＨ６，５
以上に調整することによりキトサンが析出を起し、夾雑
物とは分離して析出する。この析出物は遠心分離又は濾
布による濾過によって容易に分離できる。The chitosan-containing solution is adjusted to pH 6 or higher, preferably pH 6.5.
By making the above adjustments, chitosan is precipitated and separated from impurities. This precipitate can be easily separated by centrifugation or filtration with a filter cloth.
従来、キトサンの精製がゲル濾過等によってきわめて低
収率にしか行なわれていなかったことからみれば、ｐＨ
の調整だけでキトサンの析出が完全に起るということは
きわめて意外なことである。Conventionally, chitosan was purified by gel filtration, etc., with extremely low yields.
It is extremely surprising that the precipitation of chitosan can occur completely just by adjusting the .
分離した析出物は夾雑物の一部を混在させているので、
これを水や溶媒で洗滌して酸に溶解する。The separated precipitate contains some impurities, so
This is washed with water or a solvent and dissolved in acid.
酸としては酢酸などの有機酸、塩酸、硫酸などの無機酸
などいずれの酸でもよく、また、濃度としては０．０１
〜３モル程度のものがよい。The acid may be any acid such as an organic acid such as acetic acid or an inorganic acid such as hydrochloric acid or sulfuric acid, and the concentration is 0.01
It is preferable that the amount is about 3 mol.
析出物を酸に溶解した後は、ｐＨ６以上、好ましくはｐ
Ｈ６．５以上の処理のみで容易に析出するようになって
いるので、カセイソーダ等のアルカリを添加し、ｐＨ６
以上、好ましくはｐＨ６，５以上にＰＨ調整し、析出物
を得る。After dissolving the precipitate in acid, the pH should be 6 or more, preferably p
Since it is easy to precipitate only by treatment at pH 6.5 or higher, add an alkali such as caustic soda and raise the pH to 6.5.
As described above, the pH is preferably adjusted to 6.5 or higher to obtain a precipitate.
更に、精製するためには、この析出物を水等で洗滌し、
再び酸に溶解し、ｐＨ６以上、好ましくはｐＨ６，５以
上のｐＨ調整を行い、析出物を得ることができる。Furthermore, in order to purify this precipitate, wash it with water etc.
A precipitate can be obtained by dissolving in an acid again and adjusting the pH to 6 or higher, preferably 6.5 or higher.
この精製処理は何度でも行うことができ、はとんど純粋
なキトサンを得ることが可能となったのである。This purification process can be repeated any number of times, making it possible to obtain extremely pure chitosan.
次に本発明の試験例及び実施例を示す。Next, test examples and examples of the present invention will be shown.
試験例
市販のキトサン２５ｇを０．２５モルの塩酸５Ｑに溶解
し、溶解液を１００ｎ＋ Ｑづつ１１本用意し、第１図
のｐ）ｌ調整を行い、析出物の量を測定した。得られた
結果は図１に示される。Test Example 25 g of commercially available chitosan was dissolved in 0.25 mol of hydrochloric acid 5Q, 11 bottles of 100n+Q of the solution were prepared, p)l adjustment in Figure 1 was carried out, and the amount of precipitate was measured. The results obtained are shown in FIG.
第１図のキトサンの回収率は、溶解したキトサン１００
ｍＱ中のグルコサミン量（４Ｎ塩酸で加水分解後エルラ
ン−モルガン法で測定）に対する析出物の乾燥重量を百
分率で表示した。The recovery rate of chitosan in Figure 1 is 100% of dissolved chitosan.
The dry weight of the precipitate was expressed as a percentage with respect to the amount of glucosamine in mQ (measured by the Erlan-Morgan method after hydrolysis with 4N hydrochloric acid).
その結果は第１図に示されるが、第１図からｐＨ６を超
えるとキトサンの析出がはじまり、ｐＨ６，５以上とな
るとほとんど完全に析出するのがわかる。The results are shown in FIG. 1, and it can be seen from FIG. 1 that chitosan begins to precipitate when the pH exceeds 6, and almost completely precipitates when the pH exceeds 6.5.
実施例１
市販のキトサン２５ｇを０．５モルの塩酸５Ｑに溶解し
、不溶物を遠心分離（２０，０ＯＯＧ） して除いた。Example 1 25 g of commercially available chitosan was dissolved in 0.5 mol of hydrochloric acid 5Q, and insoluble materials were removed by centrifugation (20.0 OOG).
得られた清澄液に４０％カセイソーダを加え、ｐＨを７
．０に調整した。生じた析出物をサラン製の布で濾過し
た後乾燥し、白色粉末状のキトサン２２ｇを得た。精製
前後のキトサン中のグルコサミン量をそれぞれ、４Ｎ塩
酸で加水分解後エルラン−モルガン法で測定し、乾物に
対する含量で示すと、精製前９２％であったのに対し、
精製後は９６％であった。Add 40% caustic soda to the resulting clear liquid to adjust the pH to 7.
．． Adjusted to 0. The resulting precipitate was filtered through Saran cloth and dried to obtain 22 g of white powdered chitosan. The amount of glucosamine in chitosan before and after purification was measured by the Erlan-Morgan method after hydrolysis with 4N hydrochloric acid, and when expressed as a content on dry matter, it was 92% before purification, whereas before purification, it was 92%.
After purification, it was 96%.
実施例２
市販のキトサン１００ｇを０．５モルの酢酸２５Ωに溶
解し、不溶物を遠心分離（２０，０ＯＯＧ） して除い
た。Example 2 100 g of commercially available chitosan was dissolved in 0.5 mol of acetic acid 25Ω, and insoluble matter was removed by centrifugation (20.0 OOG).
得られた清澄液に４０％カセイソーダを加え、ｐＨを８
．５に合せた。静置後生じた析出物をサラン製の布上に
集め、水で十分洗滌し、可溶物を除去した後、乾燥して
白色粉末状のキトサンを得た・精製前後のキトサンの性
状、分析値を表１に示した。Add 40% caustic soda to the resulting clear liquid to adjust the pH to 8.
．． Adjusted to 5. After standing, the precipitate formed was collected on Saran cloth, thoroughly washed with water to remove soluble materials, and dried to obtain chitosan in the form of a white powder.Characteristics and analysis of chitosan before and after purification The values are shown in Table 1.
表中の色は日本重色工業社製の測色色差計で測定し、Ｌ
、ａ、ｂで示した。香はカニ殻臭の残存の程度を官能検
査によって示した。グルコサミンの含有量は精製前後の
キトサンを４Ｎ塩酸で加水分解した後エルラン−モルガ
ン法で測定し、算出し、乾物に対する％で表示した。The colors in the table are measured with a colorimeter made by Nihon Heavy Industries Co., Ltd.
, a, b. The degree of residual crab shell odor was determined by a sensory test. The content of glucosamine was calculated by hydrolyzing chitosan before and after purification with 4N hydrochloric acid and then measured by the Erlan-Morgan method, and expressed as a percentage of the dry matter.
表１
表１より明らかなように色は明度（Ｌ）が大きくなり、
赤色（、）が減少し、黄色（ｂ）も減少し、全体に色素
が除去され、白色になったことがわかる。Table 1 As is clear from Table 1, the lightness (L) of colors increases,
It can be seen that the red color (,) has decreased and the yellow color (b) has also decreased, indicating that the pigment has been completely removed and the color becomes white.
香りについても臭気が除去されている。そして、グルコ
サミン含有量が上がり、不純物をほとんど含まないほぼ
純品に近いキトサンが得られた。Regarding fragrance, odors are also removed. The glucosamine content increased, and almost pure chitosan containing almost no impurities was obtained.
実施例３
良く洗滌し、乾燥したズワイガニの殻３ｋｇを公知の方
法（Ｍａｋｒｏｍｏｌ、 Ｃｈｅｍ、 ｖｏｌ、 １７
７３５８９（１９７６））に準じ処理することにより粗
キトサン乾燥物約８００ｇを得た。Example 3 3 kg of well-washed and dried snow crab shells were prepared using a known method (Makromol, Chem, vol. 17).
73589 (1976)) to obtain about 800 g of crude dried chitosan.
この、粗キトサン５０ｇを０．５Ｎ酢酸１０Ｑに溶解し
、不溶解物を遠心分離して除去した。次に４０％カセイ
ソーダを用いｐＨを７．５に調整した。生じた析出物を
遠心分離して集めた。次いでこの沈澱を水に分散させ、
水に可溶物を除いた。更に、この析出物を０．５モルの
酢酸に溶解し、４０％カセイソーダでｐＨを７．５に合
わせた。生じた析出物をサラン製の布上に集め回収し、
大量の水で洗滌することにより可溶物を除いた。洗滌後
、析出物を乾燥して白色粉末状の無臭のキトサン４２ｇ
を得た。50 g of this crude chitosan was dissolved in 10Q of 0.5N acetic acid, and undissolved matter was removed by centrifugation. Next, the pH was adjusted to 7.5 using 40% caustic soda. The resulting precipitate was collected by centrifugation. This precipitate is then dispersed in water,
Water-soluble materials were removed. Further, this precipitate was dissolved in 0.5 mol of acetic acid, and the pH was adjusted to 7.5 with 40% caustic soda. Collect and collect the resulting precipitate on a saran cloth,
Soluble materials were removed by washing with a large amount of water. After washing, dry the precipitate to obtain 42g of odorless chitosan in the form of white powder.
I got it.
粗精製物及び精製物の性状、分析値は表２に示す。The properties and analytical values of the crude and purified products are shown in Table 2.
表中の色は日本重色工業社製の測色色差計で測定し、Ｌ
、ａ、ｂで表示した。香はカニ殻臭の残存の程度を官能
検査によって示した。グルコサミンの含有量はそれぞれ
のキトサンを４Ｎ塩酸で加水分解した後エルラン−モル
ガン法で測定し、算出し、乾物に対する％で表示した。The colors in the table are measured with a colorimeter made by Nihon Heavy Industries Co., Ltd.
, a, b. The degree of residual crab shell odor was determined by a sensory test. The glucosamine content was calculated by hydrolyzing each chitosan with 4N hydrochloric acid and then measuring by the Erlan-Morgan method, and expressed as % of dry matter.
表より明らかなように色は明度（Ｌ）が大きくなり赤色
（、）が減少し、黄色（ｂ）も減少し、色素が除去され
全体に白色になったことがわかる。香りについても無臭
になった。そしてグルコサミン含有量も上がり、不純物
をほとんど含まないほぼ純品のキトサンが得られた。As is clear from the table, the lightness (L) increases, the red color (,) decreases, and the yellow color (b) decreases, indicating that the pigment was removed and the overall color became white. The fragrance has also become odorless. The glucosamine content also increased, and almost pure chitosan containing almost no impurities was obtained.
第１図は試験例において、各ｐｏにおけるキ１〜サンの
析出物の乾燥重量の百分率を示す図である。FIG. 1 is a diagram showing the percentage of the dry weight of the precipitates of Ki1-San in each po in the test example.
Claims (3)
Ｈ６．５以上に調整し、析出物を生成せしめることを特
徴とするキトサンの精製法。(1) Adjust the chitosan-containing liquid to pH 6.0 or higher, preferably p
A method for purifying chitosan, which comprises adjusting H to 6.5 or higher and producing a precipitate.
Ｈ６．５以上に調整し、得られた析出物を必要に応じて
洗滌し、該析出物を酸に溶解することを特徴とするキト
サンの精製法。(2) Adjust the chitosan-containing liquid to pH 6.0 or higher, preferably p
A method for purifying chitosan, which comprises adjusting H to 6.5 or higher, washing the obtained precipitate as necessary, and dissolving the precipitate in an acid.
Ｈ６．５以上に調整し、得られた析出物を必要に応じて
水洗し、該析出物を酸に溶解し、ｐＨ６．０以上、好ま
しくはｐＨ６．５以上に調整し、得られた析出物を必要
に応じて洗滌し、再び析出物を酸に溶解し、必要があれ
ば上記の析出−洗滌−溶解をくり返すことを特徴とする
キトサンの精製法。(3) Adjust the chitosan-containing liquid to a pH of 6.0 or higher, preferably p
Adjust the pH to 6.5 or higher, wash the resulting precipitate with water as necessary, dissolve the precipitate in acid, adjust the pH to 6.0 or higher, preferably pH 6.5 or higher, and then the resulting precipitate A method for purifying chitosan, which comprises washing the precipitate as necessary, dissolving the precipitate in acid again, and repeating the above precipitation-washing-dissolution process as necessary.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP15979386A JPS6317901A (en) | 1986-07-09 | 1986-07-09 | Purification of chitosan |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP15979386A JPS6317901A (en) | 1986-07-09 | 1986-07-09 | Purification of chitosan |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS6317901A true JPS6317901A (en) | 1988-01-25 |
Family
ID=15701389
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP15979386A Pending JPS6317901A (en) | 1986-07-09 | 1986-07-09 | Purification of chitosan |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6317901A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH02174654A (en) * | 1988-12-26 | 1990-07-06 | Nakano Vinegar Co Ltd | Decoloring of liquid food |
| WO2001004207A1 (en) * | 1999-07-12 | 2001-01-18 | Cognis Deutschland Gmbh | Preparations containing no cross-linking agents |
| WO2001004187A1 (en) * | 1999-07-12 | 2001-01-18 | Cognis Deutschland Gmbh | Preparations that are devoid of cross-linking agents |
| WO2003066682A1 (en) * | 2002-02-07 | 2003-08-14 | Abbott Laboratories De Costa Rica Ltd | Method for deproteinization of chitosan |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5857401A (en) * | 1981-09-30 | 1983-04-05 | Agency Of Ind Science & Technol | Production of particulate porous chitosan |
| JPS61133143A (en) * | 1984-11-30 | 1986-06-20 | Fuji Boseki Kk | Heavy metal adsorbent |
-
1986
- 1986-07-09 JP JP15979386A patent/JPS6317901A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5857401A (en) * | 1981-09-30 | 1983-04-05 | Agency Of Ind Science & Technol | Production of particulate porous chitosan |
| JPS61133143A (en) * | 1984-11-30 | 1986-06-20 | Fuji Boseki Kk | Heavy metal adsorbent |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH02174654A (en) * | 1988-12-26 | 1990-07-06 | Nakano Vinegar Co Ltd | Decoloring of liquid food |
| JP2762087B2 (en) * | 1988-12-26 | 1998-06-04 | 株式会社中埜酢店 | Decolorization method for liquid food |
| WO2001004207A1 (en) * | 1999-07-12 | 2001-01-18 | Cognis Deutschland Gmbh | Preparations containing no cross-linking agents |
| WO2001004187A1 (en) * | 1999-07-12 | 2001-01-18 | Cognis Deutschland Gmbh | Preparations that are devoid of cross-linking agents |
| WO2003066682A1 (en) * | 2002-02-07 | 2003-08-14 | Abbott Laboratories De Costa Rica Ltd | Method for deproteinization of chitosan |
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